International Journal of Microbiology最新文献

{"title":"<i>Methylobacterium</i> spp. Isolated From Semiarid Soils Promote Growth and Drought Tolerance in Maize in Kenya.","authors":"Emmanuel Ehinmitan, Beenzu Siamalube, Turoop Losenge, Edward Mamati, Patrick Juma, Victoria Ngumi","doi":"10.1155/ijm/7442350","DOIUrl":"10.1155/ijm/7442350","url":null,"abstract":"<p><p>Drought significantly impacts crop growth and yield. Advances in agricultural biotechnology have enabled the use of drought-tolerant bacterial strains to mitigate the detrimental effects of drought on crops. This study, conducted during the 2024 dry season in Kiambu County, Kenya, evaluated 100 methylotrophic bacteria isolated from the rhizosphere of maize variety WE-4141 cultivated under severe drought conditions. Isolation was performed via serial dilution using ammonium mineral salt (AMS) medium supplemented with methanol as the sole carbon source, with identification confirmed through 16S rRNA gene sequencing. The isolates were assessed for plant growth-promoting and drought-tolerance properties under varying polyethylene glycol (PEG) 6000-induced osmotic stresses (-0.13, -0.50, -0.75, and -1.3 MPa). Seed priming was used to introduce bacterial inoculants at 10⁸ CFU/mL concentration. Under PEG-induced stress, strains K19 and K2 produced the highest quantities of gibberellic acid (GA) and indole acetic acid (IAA), with K2 also showing superior exopolysaccharide (EPS) production at the highest osmotic level. Enhanced biofilm formation was observed under drought conditions compared to controls. Antioxidant enzyme activity correlated positively with increasing PEG concentrations, with K19 exhibiting the highest catalase activity. In planta, drought stress significantly reduced plant growth, with uninoculated controls showing notable decreases in plant height, root, and shoot dry weights. Inoculation with K2 and K19 enhanced maize performance under drought, with their combination yielding the greatest improvements: shoot dry weight increased by 42.0%, root dry weight by 46.2%, and plant height by 65.8% compared to controls. These findings suggest that strains K2 and K19 hold potential as biostimulants for improving maize resilience and productivity under drought conditions and warrant further evaluation through field trials for potential bioformulation development.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"7442350"},"PeriodicalIF":3.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug-Resistant Virulent <i>Escherichia coli</i> Strains in Cattle: Implications on Food Safety and Public Health.","authors":"Ajay Kumar, Emmanuel W Bumunang, Peter Kotsoana Montso, Smriti Singh, Collins Njie Ateba","doi":"10.1155/ijm/9956724","DOIUrl":"10.1155/ijm/9956724","url":null,"abstract":"<p><p><i>Escherichia coli</i> inhabit the gastrointestinal tract of mammals, including cattle, where they occur as commensals. However, some strains have evolved as highly virulent pathogens that also harbor a variety of multidrug-resistant determinants. In the present study, from cattle fecal samples, a total of 636 confirmed <i>E. coli</i> strains were obtained based on the presence of the <i>uidA</i> housekeeping gene. Of the seven antibiotics tested, 120 isolates displayed multiple antibiotic-resistant (MAR) traits, with two strains (ERO138 and EKL68) showing resistance to six antibiotics. The <i>hlyA</i> (62.5%) was the most prevalent among the MAR isolates. In addition, 11 (9.1%) isolates harbored all four screened virulence genes (<i>eaeA</i>, <i>stx1</i>, <i>stx2</i>, and <i>hlyA</i>). Seven of the 120 MAR isolates displayed moderate biofilm-producing properties, and two of these isolates (ERO157 and EKL127) harbored all four virulence genes tested. Pulsed field gel electrophoresis (PFGE) analysis revealed that all 120 MAR isolates clustered into eight groups, displaying high genetic variability. These findings are important for screening and monitoring of diverse <i>E. coli</i> isolates from cattle in the Northwest region of South that harbors virulence and multiple antibiotic resistance traits.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"9956724"},"PeriodicalIF":3.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12422852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Antimicrobial Potential of Marine Sponges (<i>Carteriospongia foliascens</i>, <i>Callyspongia fallax</i>, and <i>Paratetilla arcifera</i>) from Kenyan Marine Waters.","authors":"Teresia Nyambura Wacira, Huxley Mae Makonde, Joseph Nyingi Kamau, Cromwell Mwiti Kibiti","doi":"10.1155/ijm/4208163","DOIUrl":"10.1155/ijm/4208163","url":null,"abstract":"<p><p>Emerging and re-emerging infectious diseases and pathogens present a significant global public health threat that has led researchers to focus on discovering new antimicrobial agents in order to address the challenge. Sponges are a promising source of marine natural products, which can be used as lead molecules for drug discovery. This study was aimed at identifying marine sponges through morphological and molecular techniques and evaluate the bioactivity potential of their organic crude extracts against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Candida albicans.</i> Deoxyribonucleic acid (DNA) barcoding of the cytochrome c oxidase subunit I (COI) gene identified three genera of sponges (<i>Carteriospongia</i>, <i>Callyspongia</i>, and <i>Paratetilla</i>). Disk diffusion assay was used to determine the inhibition zone diameter (IZD) of the sponges' extracts. Minimum inhibitory concentrations (MICs) and the minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of the most active sponge extracts were determined. The bioactive compounds were analyzed using gas chromatography-mass spectrometry (GC-MS). The dichloromethane extracts of <i>Carteriospongia foliascens</i> demonstrated the highest antifungal activity against <i>C. albicans</i> (31.33 ± 1.2 mg mL^-1), surpassing the standard drug fluconazole (29.33 ± 1.5 mg mL^-1). The MIC values for the sponge extracts ranged from 3.86 to 5.89 mg mL^-1, and the ethyl acetate extract of <i>Callyspongia fallax</i> had an MBC of 4.03 mg mL^-1 against <i>S. aureus</i>. GC-MS chromatogram identified 98 compounds across 41 classes in three sponge extracts. Notably, 9.2% of these compounds have been reported to exhibit antimicrobial activity against human pathogens. This study confirms that sponges are a source of useful biochemicals, which have potential for drug discovery. To the best of our knowledge, this is the first comprehensive study to report on the characterization of marine sponges from the Kenyan waters. Further research work to structurally elucidate and identify the most active bioactive compounds from the extracts of <i>C. foliascens</i> and <i>C. fallax</i> is recommended.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"4208163"},"PeriodicalIF":3.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Antimicrobial Resistance Patterns, Type VI Secretion System, and Virulence Factors in Clinical Isolates of <i>Klebsiella pneumoniae</i>: A Cross-Sectional Study.","authors":"Mohammad Hossein Haddadi, Elahe Taki, Abbas Maleki, Jamshid Mashhadi, Fatemeh Shahi, Maryam Koupaei, Nourkhoda Sadeghifard, Reza Faraji, Melika Moradi, Hassan Valadbeigi, Saeed Khoshnood","doi":"10.1155/ijm/9634257","DOIUrl":"10.1155/ijm/9634257","url":null,"abstract":"<p><p><b>Background:</b> <i>Klebsiella pneumoniae</i> is an opportunistic pathogen linked to a range of severe infections. It is classified into classic <i>K. pneumoniae</i> (cKp) and hypervirulent <i>K. pneumoniae</i> (hvKp) forms, with hvKp showing enhanced virulence. This study examines Type VI secretion system (T6SS) presence and virulence factors in cKp and hvKp strains while assessing antimicrobial resistance. <b>Methods:</b> Eighty-three <i>K. pneumoniae</i> isolates were collected from hospitalized patients in Ilam, Iran, between June and December 2023. Antimicrobial susceptibility testing was conducted using the disk diffusion method. Hypervirulent <i>K. pneumoniae</i> isolates were identified by string test, tellurite resistance, and presence of <i>peg-344</i>, <i>iucA</i>, and <i>iutA</i> using polymerase chain reaction (PCR). Furthermore, PCR detected virulence, <i>β</i>-lactamases, and T6SS genes. <b>Results:</b> Of 83 isolates, 16.87% and 83.13% were hvKp and cKp, respectively. All isolates were multidrug-resistant (MDR), with 18.07% exhibiting carbapenem resistance. The pattern of antibiotic resistance in hvKp and cKp is not significantly different, except for ciprofloxacin, tetracycline, and azithromycin. Also, there was no significant difference between T6SS-positive and T6SS-negative isolates except for ciprofloxacin. T6SS presence did not significantly correlate with virulence or resistance genes, except for ciprofloxacin resistance (<i>p</i> = 0.0219). <b>Conclusion:</b> Surveillance, infection control, and targeted therapies are essential to combat hypervirulent and drug-resistant <i>K. pneumoniae</i>.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"9634257"},"PeriodicalIF":3.2,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144953353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotyping of <i>Acanthamoeba</i> From Different Wards of Gonabad Bohlool Hospital, Northeastern Iran.","authors":"Mitra Salehi, Adel Spotin, Mina Moradi, Morteza Rostamian, Hassan Reza Rokni","doi":"10.1155/ijm/8672955","DOIUrl":"10.1155/ijm/8672955","url":null,"abstract":"<p><p><b>Background:</b> <i>Acanthamoeba</i> is a free-living amoeba that is widely found in nature in different environments such as soil, water, and dust. This parasite is the cause of amoebic keratitis and granulomatous amoebic encephalitis. This study is aimed at investigating the prevalence and genotypes of <i>Acanthamoeba</i> in different wards of Gonabad Bohlool Hospital, northeastern Iran. <b>Methods:</b> One hundred and eighty-three samples were collected from equipment in various wards of Gonabad hospital in 2023; swabs were cultured in nonnutrient agar (NNA) medium with the addition of killed <i>Escherichia coli</i>. In positive samples containing stellate cysts, PCR molecular testing was performed using JDP1 and JDP2 primers. To confirm <i>Acanthamoeba</i>, genotyping and sequencing were also done. <b>Results:</b> <i>Acanthamoeba</i> sp. was observed in 114 out of 183 samples (62.30%). The highest percentage of <i>Acanthamoeba</i> contamination was in the emergency ward with 81.82%, and the lowest percentage was in the operating and imaging room with 50%. Moreover, the highest percentage of <i>Acanthamoeba</i> contamination was in staff areas and equipment with 66.67%, and the lowest percentage was 56.14% on medical equipment. Also, in this research, the Genotypes T4 (<i>n</i> = 10, 43.5%), T3 (<i>n</i> = 4, 17.4%), T5 (<i>n</i> = 4, 17.4%), T11 (<i>n</i> = 3, 13%), and T2 (<i>n</i> = 2, 8.7%) were determined. <b>Conclusions:</b> The findings of this study showed that <i>Acanthamoeba</i> is more common in the emergency ward and on the surfaces and equipment of the staff areas. Considering the dangerous complications caused by this amoeba, health education to increase awareness in the field of transmission as well as health measures to prevent contamination, including disinfection, is recommended.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"8672955"},"PeriodicalIF":3.2,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144953308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cross-Sectional Study of Extended-Spectrum <i>β</i>-Lactamase-Producing <i>Escherichia coli</i> Isolated From Clinical Samples: Single-Center Investigation in Indonesia.","authors":"Fitrotin Azizah, Dita Artanti, Yety Eka Sispita Sari, Anindita Riesti Retno Arimurti, Arya Iswara, Afifah Khairunnisa, Muhammad Evy Prastiyanto, Daniel Geleta","doi":"10.1155/ijm/3743202","DOIUrl":"10.1155/ijm/3743202","url":null,"abstract":"<p><p>The emergence of extended-spectrum beta-lactamase-producing <i>Escherichia coli</i> (ESBL-producing <i>E. coli</i>) is a significant public health concern, particularly in developing countries like Indonesia, where reports on the prevalence and characteristics of these resistant strains are scarce. This lack of data hampers effective infection control and antibiotic stewardship efforts. This study is aimed at investigating the prevalence and assessing the antimicrobial susceptibility profiles of ESBL-producing <i>E. coli</i> isolated from clinical samples of Indonesian patients, thereby contributing to an understanding of antibiotic resistance patterns in this region. A cross-sectional study was conducted at RSUD dr. Adhyatma Hospital, Semarang, Indonesia, over 3 years (from January 2022 to December 2024). Clinical specimens were obtained from patients diagnosed with <i>E. coli</i> infections, and isolates were identified and assessed for antibiotic susceptibility utilizing the VITEK2 Compact system. Data were examined via the Fisher's exact test. Out of 449 <i>E. coli</i> isolates, 199 (44.3%) were identified as ESBL, with the highest prevalence in pus (35.6%) and urine (27.2%). ESBL-producing <i>E. coli</i> isolates demonstrated high sensitivity (above 90%) to amoxicillin-clavulanic acid, ceftazidime, ertapenem, meropenem, and tigecycline. Our study also underlined the higher prevalence of multidrug resistance (MDR) in ESBL compared to non-ESBL. The results highlight the urgent need for enhanced surveillance and infection control measures in healthcare settings to combat the spread of ESBL-producing <i>E. coli</i>. Healthcare professionals, including nurses and clinicians, must be aware of this resistance pattern to guide empirical treatment choices and improve patient outcomes in managing infections caused by these resistant strains.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"3743202"},"PeriodicalIF":3.2,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144953361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HWP1 Gene Sequence Diversity and Antifungal Susceptibility Patterns in Colombian Vulvovaginal Isolates of the <i>Candida albicans</i> Species Complex.","authors":"Soraya Morales-López, Yeneiris Villero Wolf, Yulibeth Torres, Deyner Lechuga, Luis Caicedo, Guillermo García-Effron","doi":"10.1155/ijm/8507361","DOIUrl":"10.1155/ijm/8507361","url":null,"abstract":"<p><p><b>Background:</b> <i>Candida albicans</i> complex species are the main cause of candidiasis. <b>Objectives:</b> The purpose of this study was to report the prevalence and genetic diversity of <i>C. albicans</i> complex using hyphal wall protein 1 (HWP1) gene size polymorphism, as well as the susceptibility patterns to fluconazole and voriconazole. <b>Methods:</b> A total of 170 yeast isolates were obtained from vulvovaginal samples, and phenotypic and proteomic identification was performed. <b>Results:</b> Most clinical isolates were <i>C. albicans</i> complex (<i>n</i> = 153) followed by <i>C. glabrata</i> (<i>n</i> = 13), <i>C. parapsilosis</i> complex (<i>n</i> = 2), and <i>Pichia kudriavzevii</i> (<i>n</i> = 2). Among <i>C. albicans</i> complexes, all isolates were <i>C. albicans sensu stricto</i> and 2.61% and 4.58% were resistant to fluconazole and voriconazole, respectively. <b>Conclusions:</b> The presence of different alleles was confirmed, heterozygosity was more common than homozygosity (71.03% vs. 28.97%), and some isolates showed a homozygosis pattern not previously described. Despite these genetic diversities, no specific genotype was linked to azole resistance.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"8507361"},"PeriodicalIF":3.2,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144953375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Bioinformatic Insights Into <i>Enterococcus faecalis</i> From Retail Meats in Nigeria.","authors":"Charles Ayodeji Osunla, Ayorinde Akinbobola, Arif Elshafea, Esther Eyram Asare Yeboah, Olayemi Stephen Bakare, Aderonke Fayanju, Dorcas Oladayo Fatoba, Bright Boamah, Daniel Gyamfi Amoako","doi":"10.1155/ijm/7325430","DOIUrl":"10.1155/ijm/7325430","url":null,"abstract":"<p><p><i>Enterococcus faecalis</i> is a commensal and opportunistic pathogen increasingly recognized for its antimicrobial resistance (AMR) and zoonotic potential. This study employs whole-genome sequencing (WGS) to characterize <i>E. faecalis</i> isolates from retail meat samples, focusing on antimicrobial resistance genes (ARGs), virulence determinants, mobile genetic elements, and phylogenomic relationships. Fifty raw meat samples, including chicken (<i>n</i> = 18), beef (<i>n</i> = 17), and turkey (<i>n</i> = 15), were collected from retail markets in Akungba-Akoko, Nigeria. Confirmed isolates underwent antimicrobial susceptibility testing and WGS-based genomic analysis. Ten <i>E. faecalis</i> isolates were recovered, predominantly from chicken. All exhibited resistance to clindamycin, erythromycin, and tetracycline. Dominant AMR genes included <i>aac(6</i>⁣'<i>)-aph(2</i>⁣^″), <i>ant(6)-Ia</i>, <i>lsa(A)</i>, <i>erm(B)</i>, <i>tet(M)</i>, and <i>tet(L)</i>. Plasmid replicons <i>rep9c</i> and <i>repUS43</i> were associated with sequence types ST477 and ST16, respectively. MGEs such as IS3, IS6, IS256, and IS1380 colocalized with resistance and virulence genes. Phylogenomic analysis revealed two major lineages (ST477 and ST16) and indicated geographic clustering across African isolates. The co-occurrence of multidrug resistance, virulence factors, and MGEs in foodborne <i>E. faecalis</i> poses a public health concern due to the risk of horizontal gene transfer and zoonotic spread. These findings support the need for strengthened genomic surveillance and AMR control strategies in food systems, particularly within low- and middle-income countries.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"7325430"},"PeriodicalIF":3.2,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144953344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benchmarking 16S rRNA Gene-Based Approaches to Bacterial Taxonomy Assignment Based on Amplicon Sequencing With Illumina and Oxford Nanopore.","authors":"Carmen Hoffbeck, Danielle M R L Middleton, Nicola J Nelson, Michael W Taylor","doi":"10.1155/ijm/7563096","DOIUrl":"10.1155/ijm/7563096","url":null,"abstract":"<p><p>Research investigating the microbial community of an ecosystem or animal can involve a range of methodologies, including sequencing technology, bioinformatic software and taxonomy database. Researchers may utilise short-read sequencing on Illumina MiSeq or long-read sequencing on platforms like Oxford Nanopore to obtain different research outcomes, for example, enhanced identification of microbes at species or strain level with Nanopore. However, replicability across these techniques is not well studied, while the technique used to process reads into microbial taxa may also result in different taxonomy assignments. In this study, we analyse an existing, real-world dataset which had low genus-level identification with Illumina sequencing and analysis with the SILVA database and compare sequencing with Nanopore on the same samples. We pair this with multiple bioinformatic approaches and taxonomy databases for each sequencing technique to compare phylum- and genus-level assignments and use mock communities to identify which combination of sequencing technique, bioinformatic approach and taxonomy database provides the most accurate taxonomy. We found that Nanopore reads processed with either utilised bioinformatic approach or taxonomy database provided higher accuracy in the assignment of a mock community than any technique combination with Illumina. We also found that the Top 10 genera assigned to a real-world database were substantially different across technique combinations and varied more by taxonomy database than either bioinformatic approach or sequencing technology.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"7563096"},"PeriodicalIF":3.2,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144953363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological Potential of Lactic Acid Bacteria Isolated From Ethiopian Honey Wine, <i>Tej</i>.","authors":"Minyilal Sisay, Asnake Desalegn, Fitsum Tigu, Mogessie Ashenafi, Feng-Yan Bai, Dagim Jirata Birri","doi":"10.1155/ijm/4014096","DOIUrl":"10.1155/ijm/4014096","url":null,"abstract":"<p><p>Some members of the lactic acid bacteria (LAB) have been used as probiotics. Ethiopian honey wine, <i>Tej</i>, may be a useful source of potential probiotic bacteria. However, LABs from this source have not yet been evaluated for their probiotic properties. This study was conducted to evaluate the <i>in vitro</i> probiotic properties of LAB isolated from Ethiopian honey wine, <i>Tej</i>. For this purpose, 30 samples were collected from Southwest Ethiopia. LAB isolates were first tested for their antimicrobial activity and those with this property were evaluated for probiotic properties, such as tolerance to acid, salt, and bile, adherence properties, anticholesterol activity, antioxidant activity, and antibiotic susceptibility. The antibacterial activity of the LAB isolates against test organisms was assessed by the agar well diffusion method. Acid, salt, and bile tolerance were evaluated by the plate count method. Adhesion properties were assessed by the determination of bacterial hydrophobicity to the nonpolar solvent p-xylene. Anticholesterol was determined by measuring the remaining cholesterol in the spent broth. The antioxidant capacity was assessed by the 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging capacity, and the antibiotic susceptibility of isolates was tested by the disk diffusion method. A total of 143 LABs were isolated from <i>Tej</i> samples. The LAB count was ranged between 7.4 and 6.5 log cfu/mL. Of the 143 LAB isolates, 37 exhibited different levels of antimicrobial activity. Eight of these were identified to species level by 16S ribosomal genes sequence analysis. The greatest inhibition was against <i>Shigella boydii</i> ATCC 25931 with 19 ± 4.2 mm, and the least inhibitory activity was against <i>Escherichia coli</i> ATTC 25922 and <i>Staphylococcus aureus</i> ATTC 25913 with 8.0 ± 1.4. All except two isolates survived at pH 2 and pH 3 (18.4%-89.6%). Then, 37 isolates survived in more than 50% bile and 54%-67% adhesion capacity. The cholesterol-lowering and 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging capacities ranged from 8% to 54% and 13% to 33%, respectively. Most isolates were susceptible to antibiotics, except for one isolate that resisted all tested antibiotics. This study shows that many LABs isolated from Ethiopian honey wine, <i>Tej</i> have probiotic properties and they can be considered as probiotic candidates. We recommend evaluation of <i>in vivo</i> probiotic properties of the LAB isolates to provide strong supporting evidence.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2025 ","pages":"4014096"},"PeriodicalIF":3.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12360873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}