International Journal of Microbiology最新文献

{"title":"Bioprospecting for <i>Isoetes cangae</i> Endophytes with Potential to Promote Plant Growth.","authors":"Danielle Silveira Santos,&nbsp;Paula Veronesi Marinho Pontes,&nbsp;Analy Machado de Oliveira Leite,&nbsp;Aline Lemos Ferreira,&nbsp;Mariana de Souza,&nbsp;Thainá Dos Santos Silva Araujo,&nbsp;Henrique Fragoso Dos Santos,&nbsp;Guilherme Correa de Oliveira,&nbsp;José Augusto Bitencourt,&nbsp;Allysson Buraslan Cavalcanti,&nbsp;Rodrigo Lemes Martins,&nbsp;Francisco De Assis Esteves","doi":"10.1155/2023/5992113","DOIUrl":"https://doi.org/10.1155/2023/5992113","url":null,"abstract":"<p><p><i>Isoetes cangae</i> is a native plant found only in a permanent pond in Serra dos Carajás in the Amazon region. Plant-associated microbial communities are recognized to be responsible for biological processes essential for the health, growth, and even adaptation of plants to environmental stresses. In this sense, the aims of this work were to isolate, identify, and evaluate the properties of endophytic bacteria isolated from <i>I. cangae.</i> The bioprospecting of potentially growth-promoting endophytes required the following steps to be taken: isolation of endophytic colonies, molecular identification by 16S rDNA sequence analysis, and evaluation of the bacterial potential for nitrogen fixation, production of indole acetic acid and siderophores, as well as phosphate solubilization and mineralization. <i>Bacillus</i> sp., <i>Rhizobium</i> sp., <i>Priestia</i> sp., <i>Acinetobacter</i> sp., <i>Rossellomorea</i> sp., <i>Herbaspirillum</i> sp., <i>Heyndrickxia</i> sp., and <i>Metabacillus</i> sp., among other bacterial species, were identified. The isolates showed to be highly promising, evidencing the physiological importance for the plant and having the potential to promote plant growth.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"5992113"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10475375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Molecular Variability of <i>Alternaria solani</i> and <i>Phytophthora infestans</i> Causing Tomato Blights.","authors":"Lydia Mugao","doi":"10.1155/2023/8951351","DOIUrl":"https://doi.org/10.1155/2023/8951351","url":null,"abstract":"<p><p><i>Alternaria solani</i> and <i>Phytophthora infestans</i> cause early and late blight diseases in tomato and potato, respectively. <i>A. solani</i> can survive for more than a decade in the soil, seed, or in plant residues at optimum temperature. The pathogen exhibits high molecular and genetic variation between isolates from potato and tomato plants, in different countries. Morphological studies reveal separate conidia borne singly on simple conidiophores. Spores are elongated, muriform, beaked, septate, and dark coloured. The mycelia are branched and septate. <i>A. solani</i> demonstrated a high genetic variability among isolates originating from the United States, Greece, Cuba, Canada, Russia, Turkey, South Africa, Brazil, and China based on vegetative compatibility groups and molecular markers (random amplified polymorphic DNA markers, random amplified microsatellite markers, and amplified fragment length polymorphisms). Different morphological and molecular variations indicate the presence of variability among the isolates. On the other hand, <i>P. infestans</i> is a diploid, obligate, heterothallic, and biotrophic oomycete, whose asexual lifecycle is characterized by alternating phases of sporangia germination, hyphal growth, and sporulation. The mycelia of <i>P. infestans</i> is coenocytic, multinucleate, and aseptate although the cross walls do not form in old cultures. Sporangia are borne singly on the branch tips of the alternately branched sporangiophore. Sporangium is hyaline and lemon shaped with a papilla at the distal end. Mating types A1 and A2 with different clonal lineages have been discovered in various parts of the world indicating variation in the species.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"8951351"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9577423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Review on HIV-Associated Dermatologic Manifestations: From Epidemiology to Clinical Management.","authors":"Zeinab Mohseni Afshar,&nbsp;Azadeh Goodarzi,&nbsp;Seyed Naser Emadi,&nbsp;Ronak Miladi,&nbsp;Safoura Shakoei,&nbsp;Alireza Janbakhsh,&nbsp;Zeinab Aryanian,&nbsp;Parvaneh Hatami","doi":"10.1155/2023/6203193","DOIUrl":"https://doi.org/10.1155/2023/6203193","url":null,"abstract":"<p><p>Dermatological disorders are among the most prevalent manifestations of HIV infection/acquired immunodeficiency syndrome (AIDS). In this review, we aimed to characterize the various dermatologic presentations among HIV-infected patients with a detailed categorization of the mucocutaneous signs and symptoms, their etiopathogenic factors, and clinical management. In fact, cutaneous manifestations of HIV are quite various, ranging from AIDS-specific skin eruptions (xerosis, pruritic papular eruptions, eosinophilic folliculitis, and acne), opportunistic infections (herpes simplex, molluscum contagiosum, cutaneous leishmaniasis, bacillary angiomatosis, disseminated histoplasmosis, disseminated cryptococcosis, and zoster) to AIDS-related malignancies (Kaposi's sarcoma, lymphoma, and nonmelanoma skin cancers) and antiretroviral therapy (ART)-associated drug eruptions. We tried to classify HIV-related cutaneous presentations which can help clinicians for a better understanding of the various specific and nonspecific features of AIDS-associated cutaneous manifestations and management of the condition.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"6203193"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10368516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9936573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on <i>Salmonella</i> Isolates from Fresh Milk of Dairy Cows in Selected Districts of Wolaita Zone, Southern Ethiopia.","authors":"Isayas Asefa,&nbsp;Ermias Legabo,&nbsp;Tsegaye Wolde,&nbsp;Haben Fesseha","doi":"10.1155/2023/6837797","DOIUrl":"https://doi.org/10.1155/2023/6837797","url":null,"abstract":"<p><p><i>Salmonella</i> infections are most commonly found in animal-derived foods. From December 2021 to May 2022, the researchers conducted a cross-sectional study to determine the prevalence of <i>Salmonella</i> isolated from raw milk collected in and around Areka town, Boloso Sore Woreda, Wolaita Zone, Southern Ethiopia. A total of 151 direct udder milk samples were collected at random and examined using bacteriological methods. The overall prevalence of <i>Salmonella</i> was 9.3% (14/151). Breed, age, body condition, lactation stage, and parity were statistically significant risk factors (<i>p</i> < 0.05). Salmonellosis was more common and statistically significant in dairy cows with poor body condition and late lactation stage, as well as the Holstein Friesian crossbreed, accounting for 17.6%, 19.1%, and 17.3%, respectively. The farm's husbandry hygiene and management system, on the other hand, had no significant association with salmonellosis (<i>p</i> > 0.05). Salmonellosis was generally considered to be moderately prevalent and was one of the diseases of dairy cows in the study area that could have an impact on dairy production and have serious health and financial repercussions. As a result, improvements in milk quality maintenance and assurance are encouraged, and the need for additional research in the study area was suggested along with other ideas.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"6837797"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10849934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and Chemical Characterization of Biosurfactant Produced from a Novel <i>Pseudomonas guguanensis</i> Strain Iraqi ZG.K.M.","authors":"Zeena Ghazi Faisal,&nbsp;Mayaada Sallal Mahdi,&nbsp;Khalid H Alobaidi","doi":"10.1155/2023/1571991","DOIUrl":"https://doi.org/10.1155/2023/1571991","url":null,"abstract":"<p><p>Microbial surfactants are widely used in medical, pharmaceutical, agricultural, industrial, food, and cosmetics applications. In the present study, 85 indigenous bacteria were isolated from petroleum-contaminated soils of the Al Dourah refinery, electric power station, and electric generators in Baghdad, Iraq. Twenty nine isolates gave positive results in both blood agar and blue agar medium and were secondarily screened. One isolate was selected as a potent biosurfactant producer and molecularly identified and recorded in the NCBI GenBank nucleotide sequence database as <i>Pseudomonas guguanensis</i> strain Iraqi ZG.K.M. In optimized conditions, this strain can produce about 3.01 g/l of biosurfactant. The product could reduce the surface tension from 72 to 38 ± 0.33 mN/m and have E₂₄% of 52 ± 0.33%. This biosurfactant was preliminarily specified to be a glycolipid and characterized as a rhamnolipid with anionic nature, usually to be a monorhamnolipid as evident from TLC, FTIR, and GC-MS analyses.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"1571991"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9908352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9274805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Usage, Susceptibility Profiles, and Resistance Genes in <i>Campylobacter</i> Isolated from Cattle, Chicken, and Water Samples in Kajiado County, Kenya.","authors":"Daniel W Wanja,&nbsp;Paul G Mbuthia,&nbsp;Lilly C Bebora,&nbsp;Gabriel O Aboge,&nbsp;Brian Ogoti","doi":"10.1155/2023/8394605","DOIUrl":"https://doi.org/10.1155/2023/8394605","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;i&gt;Campylobacter&lt;/i&gt; organisms are the major cause of bacterial gastroenteritis and diarrhoeal illness in man and livestock. &lt;i&gt;Campylobacter&lt;/i&gt; is growingly becoming resistant to critically crucial antibiotics; thereby presenting public health challenge. This study aimed at establishing antimicrobial use, susceptibility profiles, and resistance genes in &lt;i&gt;Campylobacter&lt;/i&gt; isolates recovered from chicken, cattle, and cattle-trough water samples. The study was conducted between October 2020 and May 2022 and involved the revival of cryopreserved &lt;i&gt;Campylobacter&lt;/i&gt; isolates confirmed by PCR from a previous prevalence study in Kajiado County, Kenya. Data on antimicrobial use and animal health-seeking behaviour among livestock owners (from the same farms where sampling was done for the prevalence study) were collected through interview using a pretested semistructured questionnaire. One hundred and three isolates (29 &lt;i&gt;C. coli&lt;/i&gt; (16 cattle isolates, 9 chicken isolates, and 4 water isolates) and 74 &lt;i&gt;C. jejuni&lt;/i&gt; (38 cattle isolates, 30 chicken isolates, and 6 water isolates)) were assayed for phenotypic antibiotic susceptibility profile using the Kirby-Bauer disk diffusion method for ampicillin (AX), tetracycline (TE), gentamicin (GEN), erythromycin (E), ciprofloxacin (CIP), and nalidixic acid (NA). Furthermore, detection of genes conferring resistance to tetracyclines (&lt;i&gt;tet&lt;/i&gt; (O), &lt;i&gt;β&lt;/i&gt;-lactams (&lt;i&gt;bla&lt;/i&gt; &lt;sub&gt;&lt;i&gt;OXA&lt;/i&gt;-61&lt;/sub&gt;), aminoglycosides (&lt;i&gt;aph&lt;/i&gt;-3-1), (fluoro)quinolones (&lt;i&gt;gyrA&lt;/i&gt;), and multidrug efflux pump (&lt;i&gt;cmeB&lt;/i&gt;) encoding resistance to multiple antibiotics was detected by mPCR and confirmed by DNA sequencing. The correlation between antibiotic use and resistance phenotypes was determined using the Pearson's correlation coefficient (&lt;i&gt;r&lt;/i&gt;) method. Tetracyclines, aminoglycosides, and &lt;i&gt;β&lt;/i&gt;-lactam-based antibiotics were the most commonly used antimicrobials; with most farms generally reported using antimicrobials in chicken production systems than in cattle. The highest resistance amongst isolates was recorded in ampicillin (100%), followed by tetracycline (97.1%), erythromycin (75.7%), and ciprofloxacin (63.1%). Multidrug resistance (MDR) profile was observed in 99 of 103 (96.1%) isolates; with all the &lt;i&gt;Campylobacter coli&lt;/i&gt; isolates displaying MDR. All chicken isolates (39/39, 100%) exhibited multidrug resistance. The AX-TE-E-CIP was the most common MDR pattern at 29.1%. The antibiotic resistance genes were detected as follows: &lt;i&gt;tet&lt;/i&gt; (O), &lt;i&gt;gyrA&lt;/i&gt;, &lt;i&gt;cmeB&lt;/i&gt;, &lt;i&gt;bla&lt;/i&gt; &lt;sub&gt;&lt;i&gt;OXA-61&lt;/i&gt;&lt;/sub&gt; , and &lt;i&gt;aph&lt;/i&gt;-3-1 genes were detected at 93.2%, 61.2%, 54.4%, 36.9%, and 22.3% of all &lt;i&gt;Campylobacter&lt;/i&gt; isolates, respectively. The highest correlations were found between &lt;i&gt;tet&lt;/i&gt; (O) and tetracycline-resistant phenotypes for &lt;i&gt;C. coli&lt;/i&gt; (96.4%) and &lt;i&gt;C. jejuni&lt;/i&gt; (95.8%). A moderate level of concordance was observed between the Kirby-Bauer disk diffusion method (phenotypic assay)","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"8394605"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9248433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of New Delhi Metallo-<i>β</i>-Lactamase (NDM) Genes Detected from Clinical Strains of <i>Escherichia coli</i> Isolated in Ouagadougou, Burkina Faso.","authors":"Boukaré Kaboré,&nbsp;Henri S Ouédraogo,&nbsp;Oumarou Zongo,&nbsp;Ganamé Abasse Ouédraogo,&nbsp;François Tapsoba,&nbsp;Sanogo Bougma,&nbsp;Koudbi Jacob Zongo,&nbsp;Boukaré Zeba,&nbsp;Yves Traoré,&nbsp;Idrissa Sanou,&nbsp;Aly Savadogo","doi":"10.1155/2023/4813225","DOIUrl":"https://doi.org/10.1155/2023/4813225","url":null,"abstract":"<p><p>The emergence and spread of carbapenem resistance in Gram-negative bacilli such as <i>Klebsiella pneumoniae</i>, <i>Escherichia coli</i>, <i>Acinetobacter baumannii</i>, and <i>Pseudomonas aeruginosa</i> through the production of carbapenemases is a global phenomenon. It threatens patient care and leads to therapeutic impasses. This study aims to genotypically determine the prevalence of the most frequent carbapenemase genes among multidrug-resistant <i>E. coli</i> strains isolated from patients at a biomedical analysis laboratory. A total of fifty-three unduplicated <i>E. coli</i> strains isolated from patient samples with a multidrug-resistant (MDR) profile were subjected to polymerase chain reaction (PCR) testing for carbapenem resistance genes. This study allowed us to identify fifteen strains carrying resistance genes among the fifty-three <i>E. coli</i> strains. All fifteen strains produced the metallo-<i>β</i>-lactamase enzymes; this represents a rate of 28.30% of study strains. Among these strains, ten carried the NDM resistance gene, NDM and VIM genes were detected in three strains and VIM was identified in two strains of <i>E. coli</i>. However, carbapenemases A (KPC and IMI), D (OXA-48), and IMP were not detected in the strains studied. Thus, NDM and VIM are the main carbapenemases detected in the strains in our study.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"4813225"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9611219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying Bacteria with Public Health Significance from Farmed Nile Tilapia (<i>Oreochromis niloticus</i>), Zambia.","authors":"Bertha Chitambo,&nbsp;Musso Munyeme,&nbsp;Bernard Hang'ombe","doi":"10.1155/2023/6650378","DOIUrl":"https://doi.org/10.1155/2023/6650378","url":null,"abstract":"<p><p>Zambia has seen rapid development in aquaculture, and in recent years, the industry has experienced disease outbreaks where fish have increasingly become a potential contributor to emerging bacterial zoonotic diseases. The aim of this study was to identify bacterial pathogens with zoonotic potential in apparently healthy fish and water from their habitat. A total of sixty-three fish were sampled, and fifty-nine water samples were collected from the habitats of these fish. Bacteria were cultured from the internal organs of fish and water, and these were identified through standard bacteriological methods comprising morphological characterization, Gram-staining, and a panel of biochemical tests. The following bacterial pathogens with zoonotic potential were identified at a farm prevalence of <i>Aeromonas</i> (13.2%), <i>Bacillus</i> (2.1%), <i>Clostridium</i> (2.1%), <i>Escherichia coli</i> (0.7%), <i>Klebsiella</i> (6.9%), <i>Lactococcus</i> (2.1%), <i>Listeria</i> (0.7%), <i>Staphylococcus</i> (18.1%), and <i>Streptococcus</i> (0.7). Other bacteria with varying significance as fish pathogens identified included <i>Acinetobacter</i> (2.1%), <i>Aequorivita</i> (1.4%), <i>Aerococcus</i> (1.4%), <i>Bordetella</i> (2.1%), <i>Carnobacterium</i> (10.4%), <i>Citrobacter</i> (3.5%), <i>Corynebacterium</i> (1.4%), <i>Dermatophilus</i> (1.4%), <i>Enterococcus</i> (2.1%), <i>Flavobacterium</i> (4.2%), <i>Micrococcus</i> (6.9%), <i>Planococcus</i> (1.4%), <i>Proteus</i> (1.4%), <i>Pseudomonas</i> (6.3%), <i>Rhodococcus</i> (1.4%), <i>Shewanella</i> (1.4%), <i>Streptococcus</i> (0.7%), and <i>Vagococcus</i> (0.7%). The current study provides baseline information for future reference and the implementation of public health guidelines with regard to potential zoonotic diseases in fish.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"6650378"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9716809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic Susceptibility Patterns for Carbapenem-Resistant Enterobacteriaceae.","authors":"Shahnaz Armin,&nbsp;Fatemeh Fallah,&nbsp;Abdollah Karimi,&nbsp;Fereshteh Karbasiyan,&nbsp;Masoud Alebouyeh,&nbsp;Sedigheh Rafiei Tabatabaei,&nbsp;Maryam Rajabnejad,&nbsp;Roxana Mansour Ghanaie,&nbsp;Seyed Alireza Fahimzad,&nbsp;Nafiseh Abdollahi,&nbsp;Hannan Khodaei,&nbsp;Leila Azimi","doi":"10.1155/2023/8920977","DOIUrl":"https://doi.org/10.1155/2023/8920977","url":null,"abstract":"<p><p>Carbapenem is a broad-spectrum beta-lactam antibiotic considered the last choice for the treatment of antibiotic-resistant Gram-negative bacteria. Thus, the increasing rate of carbapenem resistance (CR) in Enterobacteriaceae is an urgent public health threat. This study aimed to evaluate the antibiotic susceptibility pattern of carbapenem-resistant <i>Enterobacteriaceae</i> (CRE) to new and old antibiotics. In this study, <i>Klebsiella pneumoniae</i>, <i>E. coli</i>, and <i>Enterobacter</i> spp. were collected from 10 hospitals in Iran for one year. CRE is recognized by resistance to meropenem and/or imipenem disk after identification of the collected bacteria. Antibiotic susceptibility of CRE against fosfomycin, rifampin, metronidazole, tigecycline, and aztreonam was detected by disk diffusion method and colistin by MIC. In this study, 1222 <i>E. coli</i>, 696 <i>K. pneumoniae</i>, and 621 <i>Enterobacter</i> spp. were collected from 10 hospitals in Iran in one year. Fifty-four <i>E. coli</i> (4.4%), 84 <i>K. pneumoniae</i> (12%), and 51 <i>Enterobacter</i> spp. (8.2%) were CRE. All CRE strains were resistant to metronidazole and rifampicin. Tigecycline has the highest sensitivity on CRE and levofloxacin for <i>Enterobacter</i> spp. Tigecycline showed an acceptable effectiveness rate of sensitivity on the CRE strain. Therefore, we suggest that clinicians consider this valuable antibiotic to treat CRE.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"8920977"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9970715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10820311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bambara Nut Root-Nodules Bacteria from a Semi-Arid Region of South Africa and Their Plant Growth-Promoting Traits.","authors":"Ayansina Segun Ayangbenro,&nbsp;Mohomud Rashid Adem,&nbsp;Olubukola Oluranti Babalola","doi":"10.1155/2023/8218721","DOIUrl":"https://doi.org/10.1155/2023/8218721","url":null,"abstract":"<p><p>Rhizobial nitrogen-fixing bacteria are the main inhabitants of the root nodules of legume plants. Studying the bacterial community of legume nodules is important in understanding plant growth and nutrient requirements. Culture-based technique was used to examine the bacterial community of these underground organs from <i>Vigna subterranea</i> L. Verdc (Bambara nut), an underutilized legume in Africa, for plant growth-promoting traits. In this study, Bambara nuts were planted to trap root-nodule bacteria, and the bacteria were morphologically, biochemically, and molecularly characterized. Five selected isolates were screened <i>in vitro</i> for their plant growth-promoting traits and exhibited differences in their phenotypic traits. The polymerase chain reaction (PCR) products were subjected to partial 16S rRNA gene sequencing for phylogenetic analysis. Based on 16S rRNA gene sequence, the isolates were identified as BA1 (<i>Stenotrophomonas maltophilia</i>), BA2 (<i>Chryseobacterium</i> sp.), BA3 (<i>Pseudomonas alcaligenes</i>), BA4 (<i>Pseudomonas plecoglossicida</i>), and BA5 (<i>Pseudomonas hibiscicola</i>). Results showed that four of the five isolates could produce IAA. The capability to solubilize phosphate in Pikovskaya's agar plates was positively shown by four isolates (BA2, BA3, BA4, and BA5). Three isolates could produce hydrogen cyanide while isolates BA1, BA3, BA4, and BA5 were found to have ammonia-production traits. The results suggest that these plant growth-promoting isolates can be used as inoculants for plant growth and productivity.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2023 ","pages":"8218721"},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9811988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}