Maria Heloísa de Souza Borges-Grisi, Arella Cristina Muniz Brito, Isis Morais Bezerra, Loyse Martorano-Fernandes, Yuri Wanderley Cavalcanti, Leopoldina de Fátima Dantas de Almeida
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引用次数: 0
Abstract
Objectives: The objective was to assess the antimicrobial effect of cinnamaldehyde and α-terpineol on mono-species and dual-species biofilms involved in endodontic infection. Materials and Methods: The phytoconstituents were used at a concentration of 10 mg/mL. The biofilms of Candida albicans (ATCC 90028) and Enterococcus faecalis (ATCC 29212) were developed for 7 days and evaluated by metabolic capacity analysis using MTT, cell viability analysis by CFU/mL, and phospholipase activity. The RPMI 1640 medium was used as the negative control and sodium hypochlorite 2.5% and chlorhexidine 2% were used as positive controls. Data were analyzed by a Kruskal-Wallis test and stepwise with adjusted Bonferroni for nonnormal data and an ANOVA one-way test followed by Tukey's post hoc test for normal data (α = 5%). Results: The cellular metabolism of the C. albicans and E. faecalis mono-species biofilms was reduced by cinnamaldehyde and α-terpineol (p < 0.05). For dual-species biofilm, only α-terpineol showed differences compared to the negative control (p < 0.05). The phytoconstituents showed an inhibitory effect on cell viability (CFU/mL) and phospholipase activity of biofilms, having an activity similar to sodium hypochlorite (p > 0.05). Conclusions: The phytoconstituents cinnamaldehyde and α-terpineol, at a concentration of 10 mg/mL, had an inhibitory effect on mono-species and dual-species biofilms of E. faecalis and C. albicans.
期刊介绍:
International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.