Eco-Friendly Synthesis and Characterization of Senna italica-Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial Activity.

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2025-05-08 eCollection Date: 2025-01-01 DOI:10.1155/ijm/2072594
Emad Abada, Fatimah Habib, Abdullah Mashraqi, Yosra Modafer, Wail Alsolami, Khatib Ismail, Abdullah Ali Alamri, Abadi M Mashlawi, Abdel-Rahman M Shater
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引用次数: 0

Abstract

The eco-friendly and cost-effective biological synthesis of nanomaterials is rapidly gaining attention. This study synthesized silver nanoparticles (AgNPs) using an aqueous extract of Senna italica leaves and silver nitrate (AgNO3). The synthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray diffraction (XRD). UV-Vis spectroscopy confirmed the formation of AgNPs, displaying a characteristic surface plasmon resonance peak at 445 nm. TEM and SEM analyses revealed spherical nanoparticles with sizes ranging from 12.7 to 24 nm. FTIR spectra identified bands at 1636 and 3496 cm-1, corresponding to C=O and O-H groups, indicating their role in stabilizing the nanoparticles. XRD analysis revealed diffraction planes at 111, 200, 220, and 311, consistent with the face-centered cubic structure of silver. The AgNPs demonstrated significant antimicrobial activity against fungi and Gram-negative and Gram-positive bacteria, with Escherichia coli showing the highest sensitivity (MIC = 0.014  μg/mL). SEM analysis of E. coli showed that untreated cells retained their normal morphology, whereas AgNP-treated cells appeared shriveled and deformed. These results underscore the potential of Senna italica-derived AgNPs as effective antimicrobial agents. Future studies will be aimed at investigating the detailed mechanisms underlying the effects of AgNPs on bacterial cell structure and growth.

具有广谱抗菌活性的山泻草衍生纳米银的生态友好合成与表征。
生态友好、经济高效的纳米材料生物合成技术正迅速受到人们的关注。本研究利用山泻叶和硝酸银(AgNO3)的水萃取物合成了纳米银。采用紫外可见光谱(UV-Vis)、傅里叶变换红外光谱(FTIR)、透射电子显微镜(TEM)、扫描电子显微镜(SEM)和x射线衍射(XRD)对合成的AgNPs进行了表征。紫外可见光谱证实了AgNPs的形成,在445 nm处显示出一个特征性的表面等离子体共振峰。TEM和SEM分析显示球形纳米颗粒的尺寸范围为12.7 ~ 24 nm。FTIR光谱在1636和3496 cm-1处分别识别出C=O和O- h基团,表明它们具有稳定纳米颗粒的作用。XRD分析显示,在111、200、220和311处的衍射面符合银的面心立方结构。AgNPs对真菌、革兰氏阴性菌和革兰氏阳性菌均有显著的抑菌活性,其中对大肠杆菌的敏感性最高(MIC = 0.014 μg/mL)。对大肠杆菌的扫描电镜分析显示,未经处理的细胞保持其正常形态,而agnp处理的细胞出现萎缩和变形。这些结果强调了番泻草衍生的AgNPs作为有效抗菌药物的潜力。未来的研究将旨在探讨AgNPs对细菌细胞结构和生长影响的详细机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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