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Machine Learning Models in the Detection of MB2 Canal Orifice in CBCT Images
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-25 DOI: 10.1016/j.identj.2025.02.025
Shishir Shetty , Meliz Yuvali , Ilker Ozsahin , Saad Al-Bayatti , Sangeetha Narasimhan , Mohammed Alsaegh , Hiba Al-Daghestani , Raghavendra Shetty , Renita Castelino , Leena R David , Dilber Uzun Ozsahin
{"title":"Machine Learning Models in the Detection of MB2 Canal Orifice in CBCT Images","authors":"Shishir Shetty ,&nbsp;Meliz Yuvali ,&nbsp;Ilker Ozsahin ,&nbsp;Saad Al-Bayatti ,&nbsp;Sangeetha Narasimhan ,&nbsp;Mohammed Alsaegh ,&nbsp;Hiba Al-Daghestani ,&nbsp;Raghavendra Shetty ,&nbsp;Renita Castelino ,&nbsp;Leena R David ,&nbsp;Dilber Uzun Ozsahin","doi":"10.1016/j.identj.2025.02.025","DOIUrl":"10.1016/j.identj.2025.02.025","url":null,"abstract":"<div><h3>Objectives</h3><div>The objective of the present study was to determine the accuracy of machine learning (ML) models in the detection of mesiobuccal (MB2) canals in axial cone-beam computed tomography (CBCT) sections.</div></div><div><h3>Methods</h3><div>A total of 2500 CBCT scans from the oral radiology department of University Dental Hospital, Sharjah were screened to obtain 277 high-resolution, small field-of-view CBCT scans with maxillary molars. Among the 277 scans, 160 of them showed the presence of MB2 orifice and the rest (117) did not. Two-dimensional axial images of these scans were then cropped. The images were classified and labelled as N (absence of MB2) and M (presence of MB2) by 2 examiners. The images were embedded using Google's Inception V3 and transferred to the ML classification model. Six different ML models (logistic regression [LR], naïve Bayes [NB], support vector machine [SVM], K-nearest neighbours [Knn], random forest [RF], neural network [NN]) were then tested on their ability to classify the images into M and N. The classification metrics (area under curve [AUC], accuracy, F1-score, precision) of the models were assessed in 3 steps.</div></div><div><h3>Results</h3><div>NN (0.896), LR (0.893), and SVM (0.886) showed the highest values of AUC with specified target variables (steps 2 and 3). The highest accuracy was exhibited by LR (0.849) and NN (0.848) with specified target variables. The highest precision (86.8%) and recall (92.5%) was observed with the SVM model.</div></div><div><h3>Conclusion</h3><div>The success rates (AUC, precision, recall) of ML algorithms in the detection of MB2 were remarkable in our study. It was also observed that when the target variable was specified, significant success rates such as 86.8% in precision and 92.5% in recall were achieved. The present study showed promising results in the ML-based detection of MB2 canal using axial CBCT slices.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1640-1648"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy of Ultrasonic Cleaning Products With Various Disinfection Chemistries on Dental Instruments Contaminated With Bioburden
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-25 DOI: 10.1016/j.identj.2025.02.009
Chaminda Jayampath Seneviratne, Sadaf Aiman Khan, Jessica Zachar, Zhihe Yang, Ramya Kiran, Laurence J. Walsh
{"title":"Efficacy of Ultrasonic Cleaning Products With Various Disinfection Chemistries on Dental Instruments Contaminated With Bioburden","authors":"Chaminda Jayampath Seneviratne,&nbsp;Sadaf Aiman Khan,&nbsp;Jessica Zachar,&nbsp;Zhihe Yang,&nbsp;Ramya Kiran,&nbsp;Laurence J. Walsh","doi":"10.1016/j.identj.2025.02.009","DOIUrl":"10.1016/j.identj.2025.02.009","url":null,"abstract":"<div><h3>Objectives</h3><div>The effective cleaning of reusable dental instruments that removes organic bioburden is a crucial process in infection prevention and control in dental clinics. Despite widespread use, the parameters affecting the efficacy of ultrasonic cleaning products with different chemistries remain underexplored. In the present study, we comprehensively evaluated the cleaning efficacy of commonly available cleaning detergent products against organic bioburden on dental instruments.</div></div><div><h3>Methods</h3><div>Thirteen commercially available cleaning detergent products were assessed using Browne STF Load Check Indicators under both static and ultrasonic cleaning conditions at room temperature (25°C) and warm temperature (40°C). Experiments evaluated the effect of product concentration and contact time (1, 5, 10, and 30 minutes), and the economic impact of cleaning detergent dilution. Cleaning efficacy was also tested against artificially soiled dental instruments using ProReveal fluorescence technology.</div></div><div><h3>Results</h3><div>Significant variability in cleaning efficacy among test products was observed. Optizyme Ultra (6 mL/L), Asepti Multizyme (8 mL/L), and Getinge Enzymatic Plus (20 mL/L) demonstrated superior cleaning performance, particularly when used in ultrasonic cleaners at 40°C. In general, enzymatic products consistently outperformed nonenzymatic products for the removal of organic bioburden. Products performed better with ultrasonic agitation than under static conditions, and optimal results were obtained after 10 minutes exposure time at 40°C.</div></div><div><h3>Conclusion</h3><div>The present study for the first time provides a comprehensive insight into the role of product selection, optimal concentration, temperature, and cleaning duration in maximising soil removal from dental instruments.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1632-1639"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Progress in Basic Research and Clinical Strategies for Cementum Regeneration
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-24 DOI: 10.1016/j.identj.2025.02.017
Xiaoxue Zhu , Dandan Xiang , Yiding Huo , Xiaotao He, Faming Chen, Beimin Tian, Xuan Li
{"title":"Progress in Basic Research and Clinical Strategies for Cementum Regeneration","authors":"Xiaoxue Zhu ,&nbsp;Dandan Xiang ,&nbsp;Yiding Huo ,&nbsp;Xiaotao He,&nbsp;Faming Chen,&nbsp;Beimin Tian,&nbsp;Xuan Li","doi":"10.1016/j.identj.2025.02.017","DOIUrl":"10.1016/j.identj.2025.02.017","url":null,"abstract":"<div><div>Periodontitis is a chronic inflammatory disease that inflicts damage to periodontal tissues, leading to loss of teeth, and affects systemic health. Traditional treatments can delay inflammation, whereas regeneration of the periodontal complex (periodontal ligament, cementum, and alveolar bone) can better restore periodontal tissue function. In recent years, the regeneration of alveolar bone and the periodontal ligament has been widely reviewed although cementum has received less attention. As an avascular mineralised structure around the tooth, cementum can anchor periodontal ligament fibres to the root surface, thereby connecting teeth to alveolar bone. The supporting and stabilizing effects of cementum make its regeneration vital for restoring the functionality of the periodontal tissues. In this review, we discuss advancements in basic and clinical research appertaining to cementum regeneration. We describe the molecular mechanisms that contribute to cementum regeneration thereby providing a foundation for further basic research. Finally we summarise the clinical strategies employed for cementum regeneration, including regenerative surgery and utilisation of growth factors and stem cells.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1566-1584"},"PeriodicalIF":3.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive Analysis of Orthodontic Treatment Effects on the Oral Microbiome, Metabolome, and Associated Health Indicators
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-24 DOI: 10.1016/j.identj.2025.02.014
Qin Xie , Duo Li , Chengyan Ren , Hao Liang , Ge Shi , Weihui Chen
{"title":"Comprehensive Analysis of Orthodontic Treatment Effects on the Oral Microbiome, Metabolome, and Associated Health Indicators","authors":"Qin Xie ,&nbsp;Duo Li ,&nbsp;Chengyan Ren ,&nbsp;Hao Liang ,&nbsp;Ge Shi ,&nbsp;Weihui Chen","doi":"10.1016/j.identj.2025.02.014","DOIUrl":"10.1016/j.identj.2025.02.014","url":null,"abstract":"<div><h3>Introduction and Aims</h3><div>Effects of orthodontic treatments on oral health, particularly on the microbiome and metabolome, are not well understood, and this study aims to clarify these influences using multi-omics approaches.</div></div><div><h3>Methods</h3><div>We used 16SrRNA sequencing to analyze oral microbiota and untargeted metabolomics for metabolic profiling, comparing clear aligners (CAs) and fixed appliances (FAs) in healthy and unhealthy oral environments.</div></div><div><h3>Results</h3><div>We found CAs significantly improve oral health markers—including reduced plaque accumulation, enamel demineralization, microbiome alpha diversity, and microbial heterogeneity, especially in unhealthy oral environments. Orthodontic treatment type and overall oral health status significantly altered the oral microbiota structure and metabolite composition. Notably, the effect of orthodontic methods was more pronounced on metabolome than on microbiome. There's a strong link between changes in oral microbiome, health status, and hygiene habits. For example, <em>Prevotella</em> and <em>Treponema</em> were linked to poor oral health indicators, whereas <em>Rothia, Granulicatella</em>, and <em>Streptococcus</em> were associated with good oral hygiene indicators. Machine learning analysis identified 13 key metabolites, including cholylarginine, alpha-CEHC glucuronide, 2-hydroxypentanoic acid, Cer (d17:1/6 keto-PGF1alpha), and LysoPE (15:0/0:0), which were associated with inflammatory responses and served as predictive markers for poor oral health. These metabolites were closely correlated with specific microbial species enriched in oral environment, including <em>Rothia, Prevotella</em>, and <em>Anaeroglobus</em>, suggesting their potential as biomarkers for oral health monitoring. KEGG enrichment revealed differential metabolites were significantly enriched in alkaloid biosynthesis pathways, particularly map01064, which is crucial for polyamine synthesis related to bacterial activities.</div></div><div><h3>Conclusion</h3><div>CAs significantly improve oral health markers, particularly affecting the metabolome more than the microbiome, with key metabolites and microbial species serving as potential biomarkers for oral health monitoring.</div></div><div><h3>Clinical Relevance</h3><div>This study provides comprehensive insights into interactions among orthodontic treatments, oral health status, microbial, and metabolic dynamics, offering foundation for developing personalized strategies in oral health management and orthodontic care.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1585-1598"},"PeriodicalIF":3.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ornidazole Regulates Inflammatory Response and Odontogenic Differentiation of Human Dental Pulp Cells
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-22 DOI: 10.1016/j.identj.2025.02.008
Jing Yang , Zikai Li , Chengcheng Zhang , Jiaying Xiong , Xirui Yang , Dandan Zheng , Siming Xie , Haishan Shi
{"title":"Ornidazole Regulates Inflammatory Response and Odontogenic Differentiation of Human Dental Pulp Cells","authors":"Jing Yang ,&nbsp;Zikai Li ,&nbsp;Chengcheng Zhang ,&nbsp;Jiaying Xiong ,&nbsp;Xirui Yang ,&nbsp;Dandan Zheng ,&nbsp;Siming Xie ,&nbsp;Haishan Shi","doi":"10.1016/j.identj.2025.02.008","DOIUrl":"10.1016/j.identj.2025.02.008","url":null,"abstract":"<div><h3>Aim</h3><div>This study aimed to explore the potential of ornidazole as an alternative treatment for pulpitis, focusing on its effects on human dental pulp cells (hDPCs) and macrophages. We assessed the cytotoxicity of various concentrations of ornidazole, its safety and efficacy in treating inflamed hDPCs, and its regulatory impact on inflammatory markers during inflammation.</div></div><div><h3>Materials and Methods</h3><div>Inflammation in hDPCs was induced using lipopolysaccharides (LPS), and varying doses of ornidazole were introduced. Cell proliferation, migration, inflammation regulation, and dentinogenesis under inflammatory conditions were evaluated. Additionally, macrophages were cultured with different doses of ornidazole to analyse the expression of inflammatory genes. If statistically significant differences were observed between the control and treatment groups, this was considered evidence of ornidazole's effects on hDPCs. Statistical analysis was performed using SPSS 26.0, with one-way analysis of variance and Tukey's test for comparisons. A <em>P-</em>value of &lt; 0.05 was considered statistically significant.</div></div><div><h3>Results</h3><div>Ornidazole influenced cell proliferation, inflammation regulation, and dentinogenesis. Concentrations below 10 µg/mL did not exhibit significant cytotoxic effects on hDPCs over a 7-day period, and the cytotoxicity of ornidazole was both concentration- and time-dependent. Ornidazole decreased the expression of proinflammatory markers (IL-6 and TNF-α) while enhancing the expression of anti-inflammatory markers (IL-1Ra and IL-8). It also suppressed alkaline phosphatase (ALP) activity but increased the expression of odontogenic differentiation markers at both mRNA and protein levels in the presence of inflammatory stimuli. Furthermore, ornidazole demonstrated immunomodulatory effects on macrophages.</div></div><div><h3>Conclusions</h3><div>Low concentrations of ornidazole were found to be safe for hDPCs. Ornidazole modulated the expression of inflammatory markers (IL-6, TNF-α, IL-8, IL-1Ra) in inflamed hDPCs and regulated odontogenesis-related markers. Furthermore, low concentrations of ornidazole enhanced the immune regulation in macrophages, highlighting its potential as a therapeutic agent for pulpitis.</div></div><div><h3>Clinical Relevance</h3><div>This study aimed to understand the interactions of ornidazole with hDPCs, its anti-inflammatory properties, and its regulatory effects on odontogenic processes. By examining the impact of different concentrations of ornidazole on cells associated with pulp inflammation, this study provides valuable insights into its therapeutic potential for pulpitis and tends to support its clinical application.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1522-1531"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reassessing the Performance of Large Language Models in Oral Health Questionnaires: Interpretative Considerations
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-22 DOI: 10.1016/j.identj.2025.02.027
Carlos M. Ardila, Pradeep Kumar Yadalam
{"title":"Reassessing the Performance of Large Language Models in Oral Health Questionnaires: Interpretative Considerations","authors":"Carlos M. Ardila,&nbsp;Pradeep Kumar Yadalam","doi":"10.1016/j.identj.2025.02.027","DOIUrl":"10.1016/j.identj.2025.02.027","url":null,"abstract":"","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1564-1565"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Large Language Models Do Not Resolve Controversies Regarding Infective Endocarditis Prophylaxis
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-22 DOI: 10.1016/j.identj.2025.03.001
Pedro Diz Dios, Hoda Tayebi Hillali, Marcio Diniz Freitas, Javier Fernández Feijoo
{"title":"Large Language Models Do Not Resolve Controversies Regarding Infective Endocarditis Prophylaxis","authors":"Pedro Diz Dios,&nbsp;Hoda Tayebi Hillali,&nbsp;Marcio Diniz Freitas,&nbsp;Javier Fernández Feijoo","doi":"10.1016/j.identj.2025.03.001","DOIUrl":"10.1016/j.identj.2025.03.001","url":null,"abstract":"","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1508-1509"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association Between Helicobacter Pylori Infection and Periodontal and Gastric Diseases: A Meta-Analysis
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-22 DOI: 10.1016/j.identj.2024.12.027
Qinghua Liu , Ying Zhang , Fengxiang Qi
{"title":"Association Between Helicobacter Pylori Infection and Periodontal and Gastric Diseases: A Meta-Analysis","authors":"Qinghua Liu ,&nbsp;Ying Zhang ,&nbsp;Fengxiang Qi","doi":"10.1016/j.identj.2024.12.027","DOIUrl":"10.1016/j.identj.2024.12.027","url":null,"abstract":"<div><h3>Background</h3><div><em>Helicobacter pylori</em> (Hp) infection has been implicated in both gastric and extra-gastric diseases, including periodontitis.</div></div><div><h3>Methods</h3><div>This meta-analysis investigated the association between <em>Helicobacter pylori</em> (Hp) infection and periodontitis by systematically reviewing eight studies. The analysis was conducted using the R package “meta,” employing both fixed effects and random effects models. The level of heterogeneity among the studies was assessed using the I-squared statistic and tau values. Publication bias was evaluated through funnel plots, Begg's rank correlation, and Egger's regression tests to ensure the robustness of the findings.</div></div><div><h3>Results</h3><div>The meta-analysis included a total of 6061 observations, with 1404 cases of periodontitis. The fixed effects model produced an odds ratio (OR) of 2.3392 (95% confidence interval [CI]: 2.0207-2.7078), and the random effects model yielded a similar OR of 2.3220 (95% CI: 2.0050-2.6892). The I-squared statistic indicated low to moderate heterogeneity among the studies. Subgroup analyses demonstrated consistent associations across different Hp diagnostic methods.</div></div><div><h3>Conclusion</h3><div>Hp infection is significantly associated with an increased risk of periodontitis, highlighting its role in both gastric and oral health.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1510-1521"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Patent Analysis of Dental CBCT Machines
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-22 DOI: 10.1016/j.identj.2025.02.023
Andy Wai Kan Yeung , Andrew Nalley , Kuo Feng Hung , Anne Caroline Oenning , Ray Tanaka
{"title":"Patent Analysis of Dental CBCT Machines","authors":"Andy Wai Kan Yeung ,&nbsp;Andrew Nalley ,&nbsp;Kuo Feng Hung ,&nbsp;Anne Caroline Oenning ,&nbsp;Ray Tanaka","doi":"10.1016/j.identj.2025.02.023","DOIUrl":"10.1016/j.identj.2025.02.023","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Cone Beam Computed Tomography (CBCT) has become a crucial imaging tool in modern dentistry. At present, a review does not exist to provide comprehensive understanding of the technological advancements and the entities driving the innovations of CBCT. This study aimed to analyse the patent records associated with CBCT technology, to gain valuable insights into the trends and breakthroughs, and identify key areas of focus from manufacturers.</div></div><div><h3>Methods</h3><div>The online patent database called The Lens was accessed on 3 January 2025 to identify relevant patent records.</div></div><div><h3>Results</h3><div>A total of 706 patent records were identified and analysed. The majority of over 700 CBCT patents was contributed by CBCT manufacturers. The United States was the jurisdiction with most patent records, followed by Europe and China. Some manufacturers hold patent records for common features of CBCT systems, such as motion artifact correction, metal artifact reduction, reconstruction of panoramic image based on 3D data, and incorporation of artificial intelligence.</div></div><div><h3>Conclusion</h3><div>Patent analysis can offer valuable insights into the development and advancement of CBCT technology, and foster collaboration between manufacturers, researchers, and clinicians.</div></div><div><h3>Clinical Relevance</h3><div>The advancements in CBCT technology, as reflected by patent trends, enhance diagnostic accuracy and treatment planning. Understanding these technological innovations can aid clinicians in selecting the most effective imaging tools for patient care.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1493-1498"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-Intensity Focused Ultrasound Versus Airflow® in Debriding Ti-Attached S. mutans Biofilms
IF 3.2 3区 医学
International dental journal Pub Date : 2025-03-22 DOI: 10.1016/j.identj.2024.12.037
Minh Dien Tran, Sheetal Maria Rajan, Hien Ngo, Amr Fawzy
{"title":"High-Intensity Focused Ultrasound Versus Airflow® in Debriding Ti-Attached S. mutans Biofilms","authors":"Minh Dien Tran,&nbsp;Sheetal Maria Rajan,&nbsp;Hien Ngo,&nbsp;Amr Fawzy","doi":"10.1016/j.identj.2024.12.037","DOIUrl":"10.1016/j.identj.2024.12.037","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Effective management of biofilm-associated peri-implantitis requires the eradication of microbial biofilms to control disease progression and preserve implant osseointegration. However, achieving complete biofilm removal remains a challenge. High-intensity focused ultrasound (HIFU) is an emerging technology offering non-ionizing radiation, no extraneous materials, and minimal residuals or aerosols. This in vitro study compared the effectiveness of HIFU and a commercial air-pressured abrasive method (Airflow®) in removing <em>Streptococcus mutans</em> biofilms attached to Titanium (Ti) discs.</div></div><div><h3>Methods</h3><div>Bacterial biofilms were grown on 36 pairs of machined (M) and roughened (R) Ti discs for 10 days. Biofilms in the test group (12 pairs) were treated with the optimized HIFU for 2 min in a water medium and the residual biofilms were examined using two imaging methods, confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), and three quantitative methods including crystal violet (CV), 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and flow cytometry (FCM) assays for biomass, bacterial viability, and live/dead bacterial counts respectively. The data from the test and the control samples (untreated and Airflow® treated) were subjected to ANOVA followed by post-hoc Tukey's test to determine the statistical differences between the groups.</div></div><div><h3>Results</h3><div>Except the MTT data from M discs, the findings showed that both HIFU and Airflow® methods achieved similar levels of bacterial debridement, removing over 99% of bacteria in FCM assays (99.8 ± 0.16% versus 99.3 ± 0.49% for M discs, 99.96 ± 0.02% versus 99.5 ± 0.37% in for R discs). Complete biofilm removal was noted in HIFU-treated samples compared to the Airflow® in SEM images.</div></div><div><h3>Conclusion and clinical relevance</h3><div>Our findings indicate that both novel HIFU and traditional Airflow® methods were equally effective in removing the <em>S. mutans</em> biofilms from titanium disc surfaces. Further research is needed to explore the clinical application of HIFU in managing peri-implantitis.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1532-1543"},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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