International dental journal最新文献

{"title":"Stakeholders’ Perspectives on Quality Measurement of Oral Health Care in the Netherlands: A Qualitative Study","authors":"Amy Righolt ,&nbsp;Denise Duijster ,&nbsp;Kirsten Smits ,&nbsp;Anke Oerlemans ,&nbsp;Philip van der Wees ,&nbsp;Stefan Listl","doi":"10.1016/j.identj.2025.03.004","DOIUrl":"10.1016/j.identj.2025.03.004","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to identify which barriers and facilitators exist and can be expected when measuring quality of oral health care according to different stakeholders in the Netherlands.</div></div><div><h3>Methods</h3><div>A total of 36 semistructured interviews were conducted with dentists, patients, universities and knowledge institutes, health insurance companies, professional dental associations, and governmental health organisations. Using qualitative content analysis, barriers and facilitators were classified according to the frameworks of Grol and Cabana.</div></div><div><h3>Results</h3><div>In total 70 barrier and 53 facilitating factors were identified in the 5 domains of the frameworks. Various stakeholders found quality measurement challenging because the quality of oral health care is difficult to define with a lack of consensus on what constitutes quality of oral health care. Patients mentioned that, for them, quality of oral health care is difficult to assess. Dentists experienced a fear of being monitored and were apprehensive of the administrative burden of quality measurement. On an organisational level, the isolation of dentistry from the medical field was mentioned as a barrier. Facilitating factors were discussing quality in a trusted environment, and developing more clinical practice guidelines, which include meaningful quality measures.</div></div><div><h3>Discussion</h3><div>This study identified barriers and facilitators for measuring quality of oral health care in the Netherlands. Findings signal the importance of achieving consensus on the definition of quality of oral health care. Further strategy discussions about how quality of oral health care can be made insightful in a way acceptable to all stakeholders are needed to make progressions in quality improvement.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1722-1731"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virtual Articulator Software: Accuracy, Usability, and Clinical Applicability: A Systematic Review","authors":"Ravinder S. Saini PhD ,&nbsp;Hatim Hashim Alshoail ,&nbsp;Masroor Ahmed Kanji ,&nbsp;Sunil Kumar Vaddamanu ,&nbsp;Seyed Ali Mosaddad ,&nbsp;Artak Heboyan","doi":"10.1016/j.identj.2025.03.005","DOIUrl":"10.1016/j.identj.2025.03.005","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Virtual articulator software platforms have gained popularity in dentistry for simulating dental articulations, but a comprehensive comparison of their accuracy, user-friendliness, and clinical relevance is lacking. This study aimed to evaluate various virtual articulator software platforms, focusing on accuracy, user-friendliness, and clinical applicability.</div></div><div><h3>Methods</h3><div>A systematic search was conducted across PubMed, Google Scholar, ScienceDirect, the Cochrane Library, and Scopus, following the PICO/PEO strategy for inclusion and exclusion criteria. Studies comparing virtual articulator software platforms up to 2023 were included. Eligible studies were selected, and relevant data were extracted. The quality of the research was assessed using the QUIN tool for in vitro studies and the Mixed Methods Appraisal Tool (MMAT) tool for non-in vitro studies. Likewise, for the certainty of evidence, the Grading of Recommendation, Assessment, Development, and Evaluation (GRADE) framework was utilized.</div></div><div><h3>Results</h3><div>Out of 3,091 initially identified research articles, 17 met the inclusion criteria. The analysis revealed a diverse range of virtual articulator systems, including Panadent, Ortho CAD™, CT, CAD/CAM, PAN 300, and MATLAB program, serving as alternatives to traditional articulators. The virtual articulator software demonstrated efficiency and user-friendliness, highlighting their potential in dental applications.</div></div><div><h3>Conclusion</h3><div>The diversity of software, platforms, and systems for virtual articulators prevents the formulation of definitive and succinct conclusions. Nevertheless, in a general assessment, virtual articulators demonstrated comparable accuracy and precision when juxtaposed with their traditional counterparts.</div></div><div><h3>Clinical Relevance</h3><div>Virtual articulator software accurately simulates occlusal relationships and mandibular movements, enhancing dental treatment planning and patient outcomes. These tools integrate easily into clinical workflows, improving communication and personalized care. While further research is needed to standardize assessment methods, virtual articulators show comparable precision to traditional methods, supporting their use in diverse clinical scenarios.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1691-1704"},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Adverse Events Associated With Dental Local Anaesthetics Using Food and Drug Administration Adverse Event Reporting System Data","authors":"Ferit Bayram ,&nbsp;Ahmet Akici ,&nbsp;Asli Melda Apari ,&nbsp;Volkan Aydin","doi":"10.1016/j.identj.2025.03.002","DOIUrl":"10.1016/j.identj.2025.03.002","url":null,"abstract":"<div><h3>Objective</h3><div>Local anaesthetics (LAs), almost indispensable aspect of dental practice for pain-free procedures, occasionally leads to worrisome adverse events (AEs). We aimed to compare serious and nonserious AEs of LAs for dental reasons in the US Food and Drug Administration AE Reporting System.</div></div><div><h3>Methods</h3><div>We retrospectively analysed dental AE reports associated with LAs in the Food and Drug Administration AE Reporting System database from its inception to April 2024. We described AEs by patients’ demographic and clinical characteristics and compared ester and amide LA-associated AEs by their severity.</div></div><div><h3>Results</h3><div>We identified 1956 dental cases with a significant increase of AE reports after 2017. Lidocaine and articaine were the most commonly reported LAs (40.4% and 39.2%, respectively). Serious AEs constituted 46.2%, more commonly in females than males (56.6% vs 43.4%, <em>P</em> &lt; .001) and in those on concomitant medications (70.2% vs 42.2, <em>P</em> &lt; .001). These were also significantly higher in epinephrine-containing LAs (70.1%) than that without (34.2%, <em>P</em> &lt; .01). Ester LAs had increased risk of serious AEs (odds ratios [OR]: 3.86; 95% confidence intervals [CI]: 2.77-5.39), particularly as hospitalization, life-threatening event, or death. The odds of serious AEs were lower with lidocaine (OR: 0.59; 95% CI: 0.48-0.71) and articaine (OR: 0.63; 95% CI: 0.52-0.76).</div></div><div><h3>Conclusion</h3><div>Our study showed higher rate of serious AEs with ester LAs and those with vasoconstrictor-added practices. Besides, serious outcomes appear as more likely in females and those where additional drug use was reported.</div></div><div><h3>Clinical Relevance</h3><div>The integration of clinical evidence with pharmacovigilance data has the potential to influence clinicians’ LA preferences in dental practice, enabling more informed and evidence-based decision-making.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1705-1712"},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Infrared Thermography Predictors in Dental Implant Osteotomies: An Ex Vivo Study","authors":"Gabriel Barriga-Yauri ,&nbsp;Daniel Alvitez-Temoche ,&nbsp;Franco Mauricio ,&nbsp;Fran Espinoza-Carhuancho ,&nbsp;Ivan Calderon ,&nbsp;Julia Medina ,&nbsp;Frank Mayta-Tovalino","doi":"10.1016/j.identj.2025.03.009","DOIUrl":"10.1016/j.identj.2025.03.009","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluated the contribution of irrigation, drill type, motor, and dental implant system on infrared thermographic variations in osteotomies.</div></div><div><h3>Methods</h3><div>Osteotomy sites for 240 implants were prepared with three implant systems (Arcys, NeoBiotech and Osstem), with 80 samples analyzed in each group. Each group was further subdivided according to the drilling conditions (with and without irrigation) and motor type (Coxo, W&amp;H, Dentflex, Driller). The drill sequence included baseline, pilot, second and third drills at 1200 rpm and 40 Ncm. Infrared thermographic measurements were performed using a Fluke TiS55+ camera, with a resolution of 220 × 165 pixels and a temperature range of −20 °C to 450 °C. Statistical analysis consisted of ANOVA with Bonferroni post hoc test and linear regression model, with a view to evaluate the influence of the variables on final temperature changes.</div></div><div><h3>Results</h3><div>Irrigation caused a much lower temperature across all implant systems (<em>P</em> &lt; .001). When not irrigated, Arcys showed the highest temperatures, while in both NeoBiotech and Osstem the heat dissipated better. The Bonferroni post hoc test revealed no significant temperature difference existed among implant systems without irrigation. With irrigation, however, Arcys had a higher temperature than NeoBiotech and Osstem (<em>P</em> &lt; .001). The type of motor had no statistically significant influence on the temperature of the final drilling (<em>P</em> &gt; .05). Based on the regression analysis, the baseline, pilot, and second drill temperatures were the strongest predictors of the final drill temperature, with irrigation influencing the effects of the drill temperature.</div></div><div><h3>Conclusion</h3><div>Irrigation decreased the thermal stress during osteotomy, while NeoBiotech and Osstem showed better heat dispersing abilities. The motor type does not have a significant influence on the temperature differences. These findings establish the basic need for effective irrigation protocols to avoid thermal osteodisruption and promote osseointegration in implantology.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1683-1690"},"PeriodicalIF":3.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of an Osteoradionecrosis Model and its Mechanism Via Single Ionizing Radiation Exposure","authors":"Yuetong Wang ,&nbsp;Xian Wang ,&nbsp;Zhiqing Liu ,&nbsp;Yuetao Li ,&nbsp;Haoyu Lu ,&nbsp;Dongqin Mo ,&nbsp;Daiyou Wang","doi":"10.1016/j.identj.2025.03.006","DOIUrl":"10.1016/j.identj.2025.03.006","url":null,"abstract":"<div><h3>Introduction and Aims</h3><div>The aim of this study was to establish a reliable model of osteoradionecrosis of the mandible in New Zealand white rabbits and systematically examine the impacts of different radiation doses on mandibular tissue, as well as to appraise the inducing role of tooth extraction in the pathogenesis of the disease.</div></div><div><h3>Method</h3><div>In this research, 16 New Zealand white rabbits were randomly allocated to the control group, the 16Gy group, the 18Gy group, and the 20Gy group with a single high-dose radiation. Two weeks after radiotherapy, the teeth were extracted, and the animals were sacrificed four weeks later. Micro CT, scanning electron microscopy, HE staining, Masson staining, TRAP staining, TUNEL staining, and immunohistochemical staining were employed to verify the modelling status and damage mechanism.</div></div><div><h3>Results</h3><div>Research findings show that, compared to the control group, rabbits exposed to 18Gy and 20Gy radiation doses exhibited significant bone necrosis after tooth extraction. Key observations included extensive bone tissue necrosis, increased osteoclasts (<em>P</em> &lt; .05), reduced vascularization (<em>P</em> &lt; .001), exacerbated fibrosis (<em>P</em> &lt; .001), decreased bone density, disrupted trabecular structure, and damaged bone surface microstructure. In contrast, the 16Gy group showed some bone damage but did not meet bone necrosis criteria.</div></div><div><h3>Conclusion</h3><div>This study established an animal model appropriate for inducing ORNJ with a single large dose of radiation.</div></div><div><h3>Clinical Relevance</h3><div>This work provides a solid experimental basis and a theoretical framework for further studies of ORNJ pathogenesis in particular to explore effective preventive and clinical treatment strategies.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1672-1682"},"PeriodicalIF":3.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Probiotic Effects of Lactobacillus brevis CD2 on Caries Related Variables of Dental Plaque Biofilm","authors":"Guglielmo Campus ,&nbsp;Maria Grazia Cagetti ,&nbsp;Anna Lehrkinder ,&nbsp;Ali Alshabeeb ,&nbsp;Nicole Caimoni ,&nbsp;Peter Lingström","doi":"10.1016/j.identj.2025.02.018","DOIUrl":"10.1016/j.identj.2025.02.018","url":null,"abstract":"<div><h3>Objectives</h3><div>This study was based on the research question: “Does <em>L. brevis CD2</em> have an effect on the acidogenicity of sugar-exposed bacteria? To solve this question, a multistep study was planned: first, an in vitro investigation aimed to assess the acid production of monoculture bacterial solutions; and second, an ex vivo experiment to evaluate the production or inhibition of acids from plaque samples.</div></div><div><h3>Methods</h3><div><em>L.</em> brevis CD2 and several control strains (<em>Lactobacillus brevis CD2, Lactobacillus reuteri DSM 17938, Lactobacillus rhamnosus LB21, Lactobacillus plantarum 931, Streptococcus mutans Ingbritt</em>) were tested with various sugars; pH changes were recorded at specific time points using a micro-pH electrode. Additionally, for the ex vivo phase, the same sugars were added to equal amounts of pooled plaque from 9 healthy subjects with bacterial suspensions, as well as a control solution, and pH was monitored for up to 90 minutes. For the ex vivo phase, 9 adults were randomised in a crossover design for 28 days. For the in vivo phase, 26 healthy subjects used 1/2 lozenges 3 times daily containing either <em>L. brevis</em> CD2 (active) or no probiotic bacteria (placebo). Plaque acidogenicity was assessed using the microtouch method after a 10 ml mouth rinse containing 10% sucrose for 1 minute (on day 0 and day 28).</div></div><div><h3>Results</h3><div><em>L. brevis</em> CD2 exhibited the highest ability to inhibit the fermentation of fructose, lactose, and sucrose compared to the control strains (<em>P</em> &lt; .05). A significant reduction in plaque acidogenicity was observed in vivo from day 0 to day 28 in the test group (<em>P</em> &lt; .05).</div></div><div><h3>Conclusions</h3><div>This study indicates that <em>L. brevis</em> CD2 mitgates the acidogenic attributes of plaque biofilm organisma in vitro<em>,</em> in vivo and ex vivo, suggesting its potential benefit as a caries preventive probiotic agent.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1662-1671"},"PeriodicalIF":3.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid Dysfunction and Oral Lichen Planus: Evidence From Two-Sample Mendelian Randomization Analysis","authors":"Pengxian Xie,&nbsp;Wei Peng","doi":"10.1016/j.identj.2025.02.028","DOIUrl":"10.1016/j.identj.2025.02.028","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Epidemiological studies have shown that the association between thyroid dysfunction (TD) and oral lichen planus (OLP) is controversial, and the causal relationship remains ill-defined. This study aims to investigate their probable mutual causality using bidirectional Mendelian randomization (MR) analyses.</div></div><div><h3>Methods</h3><div>We extracted genetic instruments for OLP and 10 phenotypes of TD from the genome-wide association studies database. The inverse variance weighted method was primarily used to evaluate the bidirectional causal relationship between TD and OLP. The results’ robustness was verified by sensitivity analysis (Cochran's <em>Q</em> test, MR-Egger intercept, and leave-one-out test). Bonferroni correction threshold (0.05/10) was applied to determine significant differences.</div></div><div><h3>Results</h3><div>Forward MR analysis indicated that Hashimoto's thyroiditis (HT) was suggestively linked to a higher likelihood of developing OLP (<em>P</em> = .0077), while hypothyroidism significantly increased the risk of OLP occurrence (<em>P</em> = .0002). The reverse MR study found that OLP was suggestively related to the occurrence of hyperthyroidism (<em>P</em> = .0126) and thyroid cancer (<em>P</em> = .0244). Furthermore, OLP was found to significantly increase the risk of HT (<em>P</em> = 3.47 × 10^–⁸), Graves’ disease (<em>P</em> = 1.03 × 10^–⁵), hypothyroidism (<em>P</em> = 1.08 × 10^–⁸), and elevated thyroid-stimulating hormone levels (<em>P</em> = 1.99 × 10^–⁶). No major pleiotropy or heterogeneity was detected (<em>P</em> &gt; .05).</div></div><div><h3>Conclusion</h3><div>These findings suggest that hypothyroidism significantly increases the risk of OLP, while OLP may contribute to the development of autoimmune thyroid disorders, particularly HT, Graves’ disease, hypothyroidism, and thyroid-stimulating hormone. These findings highlight the complex interaction between endocrine and immune systems, emphasizing the need for further research into shared molecular pathways and potential clinical implications.</div></div><div><h3>Clinical Relevance</h3><div>This study provides a genetic foundation for understanding the relationship between TD and OLP, which aids early screening and diagnosis and informs therapy development.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1621-1631"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Status and Future Perspectives of Robot-Assisted Dental Implant Surgery","authors":"Shuang Huang,&nbsp;Zhongli Wang,&nbsp;Miaomiao Li,&nbsp;Yingli Song ,&nbsp;Yuezhi Li","doi":"10.1016/j.identj.2025.02.020","DOIUrl":"10.1016/j.identj.2025.02.020","url":null,"abstract":"<div><div>Dental implant surgery is now a well versed approach for tooth replacement, addressing various limitations of fixed bridges and removable dentures, thereby reinstating both the form and function of missing teeth. However, it is technically sensitive and highly dependent on the clinical experience and the expertise of the dentist. With breakthrough progress in robot-assisted surgery for a variety of systemic diseases, robot-assisted dental implant surgery has emerged as a new way to potentially enhance the efficacy of dental implant procedures. Widely researched by dental researchers, it is progressively revealing advantages in the treatment of dentition defects or edentulism. This article summarizes the current research status of robot-assisted dental implant surgery and provides a perspective grounded in the ongoing research landscape.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1608-1620"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acceptance and Willingness-to-Pay for Teledentistry: A Contingent Valuation Study","authors":"Sharon Hui Xuan Tan ,&nbsp;Ishreen Kaur Dhillon ,&nbsp;Mei Na Tan ,&nbsp;Rahul Nair ,&nbsp;Raymond Chung Wen Wong ,&nbsp;Shijia Hu ,&nbsp;Yu Fan Sim ,&nbsp;Catherine Hsu Ling Hong","doi":"10.1016/j.identj.2025.02.004","DOIUrl":"10.1016/j.identj.2025.02.004","url":null,"abstract":"<div><h3>Objectives</h3><div>The study sought to assess patients’ acceptance of teledentistry and their willingness-to-pay (WTP) for teledentistry.</div></div><div><h3>Methods</h3><div>Patients attending a dental appointment at a public hospital in Singapore were recruited to participate in a cross-sectional study between August 2021 and March 2023. Patients’ acceptance of video consults and reviews were compared using McNemar tests. A bidding game contingent valuation method with random starting points was adopted to elicit participants’ WTP for teledentistry. Logistic regression was carried out to assess participants’ WTP equivalent in-person costs by sociodemographic factors.</div></div><div><h3>Results</h3><div>Out of 597 respondents, the proportion willing to have an initial consult via a video call was 54.4% and 70.9% for a video review. Teledentistry acceptance was lower among older age groups and higher among those with higher education levels (adjusted odds ratio [adj. OR] 3.63, 95% CI = 2.16-6.15 for video reviews for university graduates). The proportion of participants willing to pay at least the quantum of in-person care for video consults and reviews was 15.2% and 79.6%, respectively. Compared to those aged 21 to 30 years, the WTP for video reviews was lower among those aged 41 years and above (OR: 0.39, 95% CI = 0.20-0.72).</div></div><div><h3>Conclusions</h3><div>Majority of patients were willing to undergo and pay at least in-person costs for video reviews compared to video consults. Younger age and higher education levels were associated with a higher willingness to accept video consults and reviews, and a higher WTP at least the equivalent in-person costs for video reviews.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1599-1607"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in Pulpitis","authors":"Xiaolan Guo ,&nbsp;Xinyan Ma ,&nbsp;Peng Liu ,&nbsp;Xiaoxin Chen ,&nbsp;Sitong Liu ,&nbsp;Longrui Dang ,&nbsp;Buling Wu ,&nbsp;Zhao Chen","doi":"10.1016/j.identj.2025.02.016","DOIUrl":"10.1016/j.identj.2025.02.016","url":null,"abstract":"<div><h3>Background</h3><div>Pulpitis is a significant dental concern characterized by inflammation of the dental pulp, often causing severe pain and potential tooth loss. Immune infiltration and necroptosis, both implicated in various inflammatory conditions, have been noted in pulpitis. However, the roles of necroptosis and immune infiltration in pulpitis pathogenesis remain poorly understood.</div></div><div><h3>Methods</h3><div>Hematoxylin and eosin (HE) staining assessed inflammatory infiltration in dental pulp tissues. Western blotting (WB) and immunohistochemistry (IHC) quantified pMLKL expression. Transcriptome datasets GSE92681 and GSE77459 from GEO identified necroptosis-related differentially expressed genes (NRDEGs) via bioinformatics analysis. Functional enrichment identified 6 hub NRDEGs based on diagnostic capability through machine learning and immune infiltration. Transwell assays evaluated neutrophil infiltration post dental pulp cells (DPCs) necroptosis. The STRING database analyzed CXCL8-activated neutrophil receptors, and Transwell and co-immunoprecipitation (Co-IP) validated ligand-receptor interactions.</div></div><div><h3>Results</h3><div>Increased pMLKL expression in inflamed dental pulp indicates activation of necroptosis pathways. Functional enrichment analysis revealed that NRDEGs were mainly involved in necroptosis. Both datasets showed significant inflammatory infiltration. Bioinformatics suggested a link between immune infiltration and necroptosis in DPCs, with correlation analyses showing NRDEGs were closely associated with neutrophil infiltration. Necroptotic DPCs produce CXCL8, which recruits neutrophils via the C3AR1 receptor. The CXCL8/C3AR1 axis, validated by Transwell and co-immunoprecipitation assays, is crucial for communication between necroptosis and neutrophil chemotaxis in DPCs.</div></div><div><h3>Conclusion</h3><div>Necroptosis occurs in pulpitis and correlates with immune cell infiltration. DPCs undergoing necroptosis can recruit neutrophils via the CXCL8/C3AR1 axis, exacerbating inflammation. This study offers new insights into the pathogenesis of pulpitis and potential therapeutic strategies.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 3","pages":"Pages 1649-1661"},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}