International dental journal最新文献

{"title":"A Novel KDF1 Variant is Associated With Multiple Natal Teeth, Tooth Agenesis, and Root Maldevelopment","authors":"John M. Graham Jr. ,&nbsp;Pedro A. Sanchez-Lara ,&nbsp;Atsushi Ohazama ,&nbsp;Katsushige Kawasaki ,&nbsp;Stefan T. Arold ,&nbsp;Piranit Nik Kantaputra","doi":"10.1016/j.identj.2025.100860","DOIUrl":"10.1016/j.identj.2025.100860","url":null,"abstract":"<div><h3>Objective</h3><div>Natal teeth are teeth that are present at birth. Multiple natal teeth are extremely rare. The objective of this study was to find the molecular aetiology of a unique dental phenotype including natal teeth, tooth agenesis, and root maldevelopment in a 5-generation family.</div></div><div><h3>Methods</h3><div>Oral and radiographic examination, linkage analysis, whole genome sequencing, and an immunohistochemical study of Kdf1 during tooth development in the mouse embryo were performed. A protein model was generated.</div></div><div><h3>Results</h3><div>We report a 5-generation family in which multiple natal teeth, oligodontia, and root maldevelopment manifested with autosomal dominant inheritance. Linkage analysis and whole genome sequencing revealed a novel pathogenic variant c.845T&gt;G; p.Ile282Ser, which cosegregated in 9 affected and 10 unaffected family members. This amino acid Ile282 is highly conserved and is important for the stabilization of a small helical fragment. This stabilization is lost in the Ile282Ser mutant, resulting in disruption of the interaction of KDF1 with its partner proteins, including IKKA, which are important for epidermal proliferation and differentiation and subsequent tooth development.</div></div><div><h3>Conclusions</h3><div>Our study demonstrates for the first time that natal teeth, tooth agenesis, and root maldevelopment are caused by a <em>KDF1</em> variant. Our study highlights the important role of <em>KDF1</em> in tooth formation and eruption.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100860"},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOX11 and GLIS2: Novel Biomarkers for Understanding the Progression of Oral Leukoplakia","authors":"Yi Li ,&nbsp;Zhencun Tang ,&nbsp;Chenliang Xiao ,&nbsp;Jun Su ,&nbsp;Lijia He ,&nbsp;Wenyun Zhang","doi":"10.1016/j.identj.2025.100872","DOIUrl":"10.1016/j.identj.2025.100872","url":null,"abstract":"<div><h3>Background</h3><div>Oral leukoplakia (LP) is a common precancerous lesion that can progress to oral squamous cell carcinoma (OSCC). Although various risk factors have been identified, the molecular mechanisms and biomarkers of LP remain insufficiently understood.</div></div><div><h3>Methods</h3><div>This study integrated single-cell RNA sequencing with bulk RNA sequencing data from leukoplakia tissues to investigate the transcriptional regulatory mechanisms within LPtissues. Our aim is to identify key molecular markers and their associated pathways. Furthermore, by analyzing survival data from OSCC in the The Cancer Genome Atlas(TCGA) database, we assessed the prognostic significance of these molecular markers in tumors. Finally, we validated the potential roles of relevant molecular markers in the progression of leukoplakia using patient LP tissue samples.</div></div><div><h3>Results</h3><div>The analysis revealed that single-cell RNA sequencing data indicated that fibroblasts in LP tissues possess a unique gene regulatory network, exhibiting significantly different transcriptional characteristics compared to normal tissues. We identified SOX11 and GLIS2 as LP-specific molecular markers, with their expression significantly elevated in LP tissues compared to normal tissues. Transcriptomic profiling of LP reveals that SOX11 and GLIS2 positively correlate with the activation of epithelial-mesenchymal transition (EMT) and angiogenesis. Additionally, analysis of OSCC samples demonstrated that high expression levels of SOX11 and GLIS2 were associated with poor prognosis. Immunohistochemical staining further showed a gradual increase in the expression of SOX11 and GLIS2 during the progression of leukoplakia, supporting their potential application as biomarkers.</div></div><div><h3>Conclusion</h3><div>As key transcription factors in LP, SOX11 and GLIS2 may influence the occurrence and development of lesions by regulating EMT and angiogenesis biological processes. They have the potential to serve as biomarkers and therapeutic targets for LP and OSCC, providing new insights into understanding the mechanisms of LP and developing treatment strategies.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100872"},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do 3D-Printed and Milled Denture Bases Differ in Microbial Activity and Adhesion? A Systematic Review and Meta-Analysis","authors":"Sarah Arzani ,&nbsp;Erfan Khorasani ,&nbsp;Aida Mokhlesi ,&nbsp;Shima Azadian ,&nbsp;Safoura Ghodsi ,&nbsp;Seyed Ali Mosaddad","doi":"10.1016/j.identj.2025.100857","DOIUrl":"10.1016/j.identj.2025.100857","url":null,"abstract":"<div><div>The increasing adoption of digital manufacturing in prosthodontics raises concerns about microbial metabolic activity and adhesion on denture bases fabricated via additive and subtractive methods. This systematic review aimed to compare microbial metabolic activity and microorganism adhesion between 3D-printed and milled complete denture bases. A systematic search of PubMed, Scopus, Web of Science, Embase, and the Cochrane Library was conducted up to December 2024. Studies were screened according to predefined eligibility criteria, and data were independently extracted by two reviewers. The standardized mean difference (SMD) was used to compare microbial metabolic activity and cell adhesion outcomes between groups. A random-effects model was applied for meta-analysis, and heterogeneity was assessed using Cochran's Q, I², and τ² indices. Publication bias was evaluated using funnel plots, Egger's test, and the Trim and Fill method. The Quality Assessment Tool for In Vitro Studies (QUIN) was used to assess the risk of bias. Twelve studies met the inclusion criteria. The meta-analysis revealed that microbial activity was significantly higher in 3D-printed compared to milled denture bases (SMD = 1.285, 95% CI: 0.810-1.760, <em>P</em> &lt; .001). Similarly, the microbial cell count was significantly higher in the 3D-printed group (SMD = 1.050, 95% CI: 0.124-1.976, <em>P =</em> .026). Surface roughness was tested as a covariate in meta-regression and was not a significant predictor of microbial activity. 3D-printed denture bases exhibited higher microbial metabolic activity and adhesion than milled counterparts. However, the heterogeneity of studies and variations in fabrication parameters necessitate further research to optimize material selection and processing protocols.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100857"},"PeriodicalIF":3.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Life Course Adiposity on Bell’s Palsy Mediated by Glucose Levels","authors":"Xin Chen ,&nbsp;Kai Zhang ,&nbsp;Zheng Cheng ,&nbsp;Qianyi Wang ,&nbsp;Zhibai Zhao ,&nbsp;Qianglin Jiang","doi":"10.1016/j.identj.2025.100867","DOIUrl":"10.1016/j.identj.2025.100867","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Previous observational studies have reported an association between obesity and Bell’s palsy (BP), but the causality and the underlying mediating pathways remain unclear. We aimed to investigate the causal effects of adiposity across the life course on BP and to identify potential mediators in this relationship.</div></div><div><h3>Methods</h3><div>Utilizing summary statistics from genome-wide association studies predominantly of European ancestry, we conducted univariable and multivariable Mendelian randomization (MR) to estimate overall and independent effects of 5 adiposity-related traits (birth weight, childhood body mass index [BMI], adult BMI, adult body fat percentage, and adult waist circumference) on BP. Two-step MR was employed to assess the mediating role of metabolic and inflammatory traits. Sensitivity analyses were performed to evaluate robustness.</div></div><div><h3>Results</h3><div>Univariable MR revealed that genetically predicted adult BMI (OR: 1.29, 95% CI: 1.11-1.49, <em>P</em> = .001), body fat percentage (OR: 1.33, 95% CI: 1.08-1.65, <em>P</em> = .008), and waist circumference (OR: 1.29, 95% CI: 1.08-1.55, <em>P</em> = .005) were significantly associated with increased risk of BP. Multivariable MR confirmed an independent causal effect of adult BMI after adjusting for birth weight and childhood BMI. Two-step MR further identified glucose levels as a partial mediator, accounting for 23.8% (95% CI: 4.9%-42.6%) of the total effect. Findings were consistent across sensitivity analyses.</div></div><div><h3>Conclusion</h3><div>Higher genetically predicted adult BMI could increase the risk of BP, partly mediated by impaired glucose regulation.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100867"},"PeriodicalIF":3.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding Cellular Landscapes: Unravelling Periodontitis and Periapical Periodontitis at the Single-Cell Frontier","authors":"Yuan Li ,&nbsp;Jun Wang ,&nbsp;Yu Zhang ,&nbsp;Dengke Li ,&nbsp;Wuyang Zhang ,&nbsp;Xuebing Li ,&nbsp;Bolun Zhang ,&nbsp;Kaijin Hu ,&nbsp;Jianfei Yan ,&nbsp;Yang Xue","doi":"10.1016/j.identj.2025.100851","DOIUrl":"10.1016/j.identj.2025.100851","url":null,"abstract":"<div><h3>Objective</h3><div>Elucidate differences in key gene expression and cellular heterogeneity in alveolar bone between periodontitis (PD) and periapical periodontitis (PP) by analysing single-cell RNA sequencing data.</div></div><div><h3>Methods</h3><div>Single-cell RNA sequencing datasets for PD (GSE171213) and PP (GSE181688) were obtained from the Gene Expression Omnibus and analysed. Differentially expressed genes were identified in comparisons of PD versus healthy controls (HC), PP versus HC, and PD versus PP. Functional enrichment analyses were performed to explore heterogeneity. Key genes related to angiogenesis (ARGs), inflammation (IRGs), and phagocytosis (PRGs) were identified and combined to form PD-specific and PP-specific key gene sets. The expression of these key genes was validated by quantitative real-time polymerase chain reaction. Cell types were annotated using SingleR, and key cell types were further analysed for their proportions, intercellular communication, and gene expression patterns. Hallmark pathway analysis and cell cycle/differentiation assessments were conducted to understand functional heterogeneity.</div></div><div><h3>Results</h3><div>A total of 251 Differentially expressed genes associated with humoral immunity, B cell regulation, and leukocyte/lymphocyte activation were identified. Four PD-specific and 16 PP-specific key genes were found, and fibroblasts and macrophages were identified as the two key cell types. Fibroblast interactions were prominent, and cells in the G1 phase predominated. The key genes were highly expressed during early T cell and macrophage differentiation and during late fibroblast and plasma cell differentiation. Expression levels of these genes were highest in PP, intermediate in PD, and lowest in HC.</div></div><div><h3>Conclusion</h3><div>This study identified four PD-specific and 16 PP-specific key genes, as well as two pivotal cell types in alveolar bone inflammatory diseases. Investigating the functional diversity of these cell types could enhance insights into the pathogenesis of these conditions.</div></div><div><h3>Clinical Relevance</h3><div>The findings may guide clinical treatment strategies for alveolar bone involvement in PD and PP, and lay a theoretical foundation for exploring the underlying inflammatory mechanisms.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100851"},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Dynamin-Related Protein 1-Dependent Mitochondrial Fission Ameliorates Apical Periodontitis by Attenuating NLRP3 Inflammasome-Mediated M1 Macrophage Polarisation","authors":"Yao Yang ,&nbsp;Min Li ,&nbsp;Lifei Pan ,&nbsp;Yifei Tang ,&nbsp;Yuting Wang ,&nbsp;Xiaoyue Guan ,&nbsp;Tiezhou Hou ,&nbsp;Siyu Hou","doi":"10.1016/j.identj.2025.100853","DOIUrl":"10.1016/j.identj.2025.100853","url":null,"abstract":"<div><h3>Introduction and Aims</h3><div>The aim of this study was to determine if Dynamin-related protein 1‌ (Drp1) -dependent mitochondrial fission is involved in the pathological process of apical periodontitis and elucidate the underlying mechanisms.</div></div><div><h3>Methods</h3><div>Immunohistochemistry, immunofluorescence, and western blotting assessed CD86 expression, NLRP3/Caspase1/IL-1β activation, and mitochondrial dynamics-related proteins in human periapical macrophages. In vitro apical periodontitis (AP) models used macrophages stimulated with <em>P. gingivalis</em> LPS (Pg-LPS), pretreated with/without Drp1 inhibitor Mdivi-1 or NLRP3 inhibitor MCC950. CD86, NLRP3/Caspase1/IL-1β, mitochondrial dynamics proteins, TNF, and IL-6 were quantified via western blot, RT-qPCR, or immunofluorescence. ROS (DCFH-DA), ATP (commercial kit), mitochondrial membrane potential (JC-1), and morphology (TEM, MitoTracker/immunofluorescence) were analysed. Multimodal approaches explored Drp1-mediated mitochondrial fission and NLRP3 inflammasome-dependent M1 polarisation. Mdivi-1-treated AP models were established to dissect Drp1’s role in pathogenesis.</div></div><div><h3>Results</h3><div>Our findings indicate that the Drp1-mediated excessive mitochondrial fission is present in human periapical lesions. Moreover, there was a positive correlation between the p-Drp1^(Ser616) and the elevation of NLRP3, Cleaved-Caspase1, and CD86. In vitro experiments demonstrated that Mdivi-1 effectively inhibited the Pg-LPS induced abnormal mitochondrial fragmentation dependent on p-Drp1^(Ser616), rescued mitochondrial dysfunction, and further suppressed the activation of NLRP3/Caspase1/IL1β and the expression of CD86. In vivo experiments showed that Mdivi-1 treatment could significantly alleviate the inflammatory bone erosion at the apex in a murine AP model by inhibiting macrophage polarisation and the NLRP3 inflammatory pathway.</div></div><div><h3>Conclusion</h3><div>Drp1-mediated excessive mitochondrial fission plays a crucial role in the development of AP by promoting NLRP3 signaling pathway-dependent macrophage M1 polarisation. Targeting Drp1 may serve as a potential therapeutic strategy to prevent AP development, highlighting its clinical translational value.</div></div><div><h3>Clinical Relevance</h3><div>Mdivi-1 can restore mitochondrial dynamics homeostasis in periapical macrophages, inhibit NLRP3/M1 polarisation, and reduce bone resorption. This suggests that Mdivi-1 might be a promising agent for management of AP in future.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100853"},"PeriodicalIF":3.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Artificial Intelligence Solution for Automated Dental Inspection and Charting","authors":"M.A. Moufti ,&nbsp;M. Abu Talib ,&nbsp;S. Al Mokdad ,&nbsp;A. Alhouria ,&nbsp;M. Alsaffarini ,&nbsp;N. Almahmeed ,&nbsp;S. Eldessouky ,&nbsp;W. Kharoufah ,&nbsp;Q. Nasir","doi":"10.1016/j.identj.2025.100849","DOIUrl":"10.1016/j.identj.2025.100849","url":null,"abstract":"<div><h3>Background</h3><div>Visual inspection and documentation (charting) of findings are critical components in dental practice. However, manual charting is tedious, time consuming, and prone to errors. Attempts to automate charting using AI have focused on computer vision of radiographs, which does not replace visual inspection and has limitations in community screening settings. We aim to develop a deep-learning-based real-time charting using intra-oral videos. This study encompasses tooth identification, the initial phase of our development process.</div></div><div><h3>Methods</h3><div>100 intra-oral videos were recorded, annotated using CVAT software, and split into training and testing datasets (80:20 ratio) to develop an object detection YOLOv7x model on a Convolutional Neural Network (CNN). The model was evaluated by its ability to accurately detect each tooth's ID (class) and position using the mean average precision (AP), which encases a confusion matrix, Precision and Recall, based on a minimum overlap of 50% between manual and automated annotation (Intersection over Union IoU = 0.5).</div></div><div><h3>Results</h3><div>Of the 186,332 captured image frames; 8747 were annotated, with data augmentation yielding 17,494 images. AP varied among tooth types; mAP at 0.5 IoU reached 78.8%, with Precision and Recall at 72.5% and 77.9% respectively.</div></div><div><h3>Conclusion</h3><div>To the best of our knowledge, this is the first study to utilize real-time videos for this purpose. This research presents a novel approach to automate dental inspection and charting, offering the potential to reduce clinical time and costs, improve patient experience and outcomes, and enhance patient access to dental care.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100849"},"PeriodicalIF":3.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value Analysis of Preoperative Peripheral Blood Markers in the Identification of Benign From Malignant Parotid Gland Tumors","authors":"Zheng Ye,&nbsp;Hui Dong,&nbsp;Fuyong Sui","doi":"10.1016/j.identj.2025.100854","DOIUrl":"10.1016/j.identj.2025.100854","url":null,"abstract":"<div><h3>Objectives</h3><div>To assess the value of preoperative peripheral blood inflammatory and nutritional parameters in distinguishing between benign and malignant parotid gland tumors.</div></div><div><h3>Methods</h3><div>A retrospective analysis was conducted on 79 patients with malignant parotid gland tumors and 79 patients with benign parotid gland tumors who were treated at the Second Hospital of Dalian Medical University from January 2015 to December 2024. Preoperative levels of total protein (TP), prealbumin (PA), albumin (Alb), albumin-to-globulin ratio (AGR), apolipoprotein A1-to-apolipoprotein B ratio (ApoA1/ApoB), and platelet-to-lymphocyte ratio (PLR) were compared between the malignant and benign tumor groups. The diagnostic value of these parameters in differentiating between benign and malignant parotid tumors was evaluated.</div></div><div><h3>Results</h3><div>The ApoA1/ApoB ratio and PLR were significantly higher in the malignant tumor group compared to the benign tumor group (<em>P &lt; .</em>05). Conversely, TP, Alb, PA, and AGR were significantly lower in the malignant tumor group than in the benign tumor group (<em>P &lt; .</em>05). Binary logistic regression analysis identified TP, PA, AGR, and ApoA1/ApoB as independent risk factors for parotid malignancy (<em>P &lt; .</em>05). The optimal cut-off values for diagnosing parotid malignancy were 65.75 for TP, 248.46 for PA, 1.55 for AGR, and 1.45 for ApoA1/ApoB.</div></div><div><h3>Conclusions</h3><div>Preoperative peripheral blood inflammatory and nutritional markers may serve as valuable indicators for differentiating between benign and malignant parotid gland tumors, providing a potential adjunctive diagnostic tool. However, further large-scale studies are required to validate these findings.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100854"},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Preclinical Postgraduate Training in Endodontic Education: A Transnational Exploratory Survey","authors":"Venkateshbabu Nagendrababu ,&nbsp;Vellore Kannan Gopinath ,&nbsp;Mohannad Nassar ,&nbsp;Naresh Shetty ,&nbsp;Anil Kishen ,&nbsp;Paul V. Abbott ,&nbsp;Henry F. Duncan","doi":"10.1016/j.identj.2025.100861","DOIUrl":"10.1016/j.identj.2025.100861","url":null,"abstract":"<div><h3>Introduction and aims</h3><div>Endodontic postgraduate programmes should offer students a wide range of experiences and training in all facets of the specialisation. This study aimed to assess the current postgraduate education practices in endodontics across dental schools worldwide.</div></div><div><h3>Methods</h3><div>The current survey consisted of an online questionnaire that had been validated and piloted. It comprised 68 questions in three sections: general information about the programme, preclinical education, and clinical education. The survey included faculty members who teach postgraduate endodontics from one dental school in each participating country. The data was presented using simple descriptive statistics.</div></div><div><h3>Results</h3><div>In total, 33 faculty members, with a response rate of 85%, from different countries completed the survey. The majority of dental schools that participated in the survey offer a 3-year postgraduate programme. The postgraduate students develop abilities in various instrumentation techniques, irrigation devices, sealers/cements, root canal filling techniques, surgery, and microscope use. Most of the schools conduct a final-year exit examination.</div></div><div><h3>Conclusions</h3><div>Postgraduate education differs globally in its preclinical and clinical training. The survey offers initial insights into postgraduate endodontic education and can help establish mutual educational standards and key focus areas.</div></div><div><h3>Clinical relevance</h3><div>The curricula for preclinical and clinical education in postgraduate endodontics programmes vary globally.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100861"},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements and Trends in Electrochemical Biosensors for Saliva-Based Diagnosis of Oral Diseases: A Bibliometric Analysis (2000–2023)","authors":"Qian Tang ,&nbsp;Fushen Zhang ,&nbsp;Weiye Xu ,&nbsp;Zejun Deng ,&nbsp;Zhangui Tang ,&nbsp;Jufang Huang","doi":"10.1016/j.identj.2025.100840","DOIUrl":"10.1016/j.identj.2025.100840","url":null,"abstract":"<div><h3>Background</h3><div>Oral diseases, such as dental caries, periodontal diseases, and oral cancers, represent significant global public health challenges. With the COVID-19 pandemic, traditional diagnostic methods have limitations, thus highlighting the need for innovative, early, and non-invasive diagnostic approaches. Analyse and summarise publications globally through a comprehensive bibliometric study to help researchers suggest possible directions for future research.</div></div><div><h3>Objective</h3><div>The purpose of the study was to evaluate global academic productivity, impact, and collaboration of electrochemical biosensors for oral diseases utilising bibliometrics based on annual number of publications, countries and regions, institution, authors, journals, citations and co-occurrences of author keywords over the last 20 years.</div></div><div><h3>Methods</h3><div>This study employs bibliometric analysis to assess the research of electrochemical biosensors for oral diseases in the Web of Science from 2000 to 2023. Utilising VOSviewer, CiteSpace software, and Microsoft Excel, we conducted a bibliometric and visualised analysis of electrochemical biosensors for oral diseases.</div></div><div><h3>Results</h3><div>The quantity of pertinent publications in this research domain displays a fluctuating but overall upward trend. In aggregate, there are 341 articles and 82 reviews, encompassing research contributions from 55 countries, 649 institutions, and involving 2068 authors. Among these publications, China, the USA, and India emerged as the predominant contributing nations. Predominantly, articles found their publication venue in “Biosensors &amp; Bioelectronics.” Notably, the author with the highest number of publications and most influence is Wang, Joseph S. The top 3 keywords include “biosensor,”“sensor,”“saliva.”</div></div><div><h3>Conclusion</h3><div>In this investigation, statistical analysis and network visualisation were conducted to reveal the research progress, trends, and trending topics on electrochemical biosensors for oral diseases via a thorough bibliometric analysis. We found that Despite these challenges, electrochemical biosensors hold significant promise for transforming oral disease diagnostics. Overcoming current technical barriers will improve both oral and systemic health outcomes.</div></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"75 4","pages":"Article 100840"},"PeriodicalIF":3.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}