International Biodeterioration & Biodegradation最新文献

{"title":"Exploring integrated bioremediation approaches on detoxification of Hg(II) in aqueous medium through a coupled bioreduction process using Bacillus stercoris (MRM1)","authors":"Bhuvaneshwari Parthasarathy ,&nbsp;Aarthi Baskaran ,&nbsp;Rajakumar Sundaram ,&nbsp;Radhakrishnan Manikkam ,&nbsp;Ayyasamy Pudukkadu Munusamy","doi":"10.1016/j.ibiod.2026.106295","DOIUrl":"10.1016/j.ibiod.2026.106295","url":null,"abstract":"<div><div>Bioremediation offers an effective solution for divalent mercury [Hg(II)] detoxification, one of the most toxic environmental pollutants affecting humans and aquatic ecosystems even at trace levels. In this study, the bacterial strain <em>Bacillus stercoris</em> MRM1 isolated from magnesite-contaminated soil tolerated up to 1 g/L of Hg(II) and achieved a 98.5% removal rate, outperforming other strains. Foggy dark spots on X-ray film confirmed Hg(II) volatilization, indicating its biotransformation to elemental mercury Hg0. Optimal parameters: pH 7, temperature 30–40 °C and 0.5% w/v starch were identified for scalability. Excessive carbon suppressed Hg(II) removal, suggesting reduced biotransformation under nutrient rich conditions. The optimized conditions were validated in lab-scale fixed-bed soil columns, where mercury removal occurred by day 3 (treat 2) and day 5 (treat 1). A pilot-scale 10 L bioreactor further demonstrated reproducibility, with treat 2 achieving faster detoxification than the non-optimized setup. AAS analysis confirmed Hg(II) levels reduced to 2 μg/L (column) and 1.5 μg/L (bioreactor) by day 10. FTIR, EDX, and SEM analyses supported the effective reduction and morphological changes in bacterial cells during remediation. Non-toxicity of treated water was verified using <em>Vigna radiate</em>, <em>Zea mays</em> and <em>Allium cepa</em> assays, showing healthy germination and mitosis comparable to control. Therefore, this study presents <em>Bacillus stercoris</em> MRM1 strain as a promising agent for scalable Hg(II) bioremediation this was highlighted by pilot-scale studies where bioremediation was executed in soil-bed column and in bioreactor studies, producing non-toxic effluents suitable for environmental reuse. This could further benefit our environment by involving in Hg(II) bioremediation in massive polluted sites.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"209 ","pages":"Article 106295"},"PeriodicalIF":4.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of biofilm fouling in filtration system based on immersed boundary method","authors":"Yumeng Fu ,&nbsp;Jiankun Wang ,&nbsp;Xiaoling Wang","doi":"10.1016/j.ibiod.2026.106289","DOIUrl":"10.1016/j.ibiod.2026.106289","url":null,"abstract":"<div><div>Biofilms are structured microbial communities formed by microorganisms (including bacteria, fungi, and other microbes) and their secreted extracellular polymeric substances (EPS), adhering to biotic or abiotic surfaces. In filtration system, biofilm formation can lead to filtration membrane clogging, water quality deterioration, equipment corrosion, and secondary pollution, among other issues. To investigate the formation and influencing factors of biofilm fouling in filtration system, we employed the immersed boundary method to simulate three typical clogging patterns: cake clogging, pore clogging, and streamer clogging. A systematic parametric analysis was conducted to elucidate the influence of key parameters, including fluid velocity, elastic modulus, channel spacing, and cell adhesion/detachment threshold, on streamer fouling formation. Our findings demonstrate that under conditions of higher fluid velocity, lower biofilm elastic modulus, and wider channel spacing, the length of streamer fouling is longer, the fluid flux is larger, and the capture efficiency of filter membrane is lower. Furthermore, we observed that the smaller the cell adhesion or detachment threshold, the more significant decline in capture efficiency, while the larger the detachment threshold, the more the flux decreases.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"209 ","pages":"Article 106289"},"PeriodicalIF":4.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic Neopestalotiopsis formicarum from a Korean native host biodegrades bisphenol A into non-cytotoxic by-products","authors":"Kumar Vishven Naveen ,&nbsp;Yang Yun Kyong ,&nbsp;Patrick Brice Defo Deeh ,&nbsp;Myeong-Hyeon Wang ,&nbsp;Anbazhagan Sathiyaseelan ,&nbsp;Hye-Yong Kim","doi":"10.1016/j.ibiod.2026.106290","DOIUrl":"10.1016/j.ibiod.2026.106290","url":null,"abstract":"<div><div>Continuous efforts are being made to remove or reduce the endocrine disruptor bisphenol A (BPA) from the environment, with mycoremediation recognized as a promising approach. This study aimed to isolate and identify endophytic and endolichenic fungi from Korean native plants and lichens capable of degrading BPA under liquid fermentation conditions. Among 15 fungal isolates, most exhibited higher biomass yield and total protein content in BPA-containing medium (CZB) compared to the control (CZY). High-performance liquid chromatography (HPLC) analysis revealed that <em>Neopestalotiopsis formicarum</em> KNUEP11 completely degraded 50 mg/L of BPA within 336 h. The fermentation filtrate (CZB) of KNUEP11 showed notable free radical scavenging activities (DPPH: 15 %, ABTS: 27 %) and minimal cytotoxicity (&lt;20 %) toward NIH3T3 cells. Furthermore, treatment of KNUEP11 fermented CZB indicated a trivial reactive oxygen species (ROS) level compared to CZB (alone) treated NIH3T3 cells. These findings suggest that <em>N. formicarum</em> KNUEP11 can efficiently degrade BPA without generating cytotoxic by-products, highlighting its potential for eco-friendly mycoremediation. However, further studies are needed to identify the metabolic pathways and degradation products involved.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"209 ","pages":"Article 106290"},"PeriodicalIF":4.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moisture dynamics during Coniophora puteana brown rot degradation of Scots pine sapwood","authors":"Tiina Belt ,&nbsp;Andreas Treu ,&nbsp;Veikko Möttönen ,&nbsp;Michael Altgen","doi":"10.1016/j.ibiod.2026.106296","DOIUrl":"10.1016/j.ibiod.2026.106296","url":null,"abstract":"<div><div>Wood has many attractive material qualities, but it is susceptible to biological degradation by wood-decaying fungi. Moisture is one of the critical requirements for wood decay, but much remains unknown about moisture dynamics in decaying wood. To fill this knowledge gap, this study investigated moisture in Scots pine sapwood during decay caused by the brown rot fungus <em>Coniophora puteana</em>. Samples were exposed to decay in two time-series experiments; mass loss and moisture content were recorded over the course of decay, and the bound and free water populations in the samples were analysed using low-field nuclear magnetic resonance (LFNMR) relaxometry in both the decaying state and at full water saturation. Selected samples were also used for water vapour sorption measurements. The time-series decay tests showed that moisture content initially increased due to fungal activity but decreased over time when corrected for mass loss, contrary to the general belief that moisture content increases with decay. LFNMR revealed that bound water content increased on a decayed-mass basis in the decaying state and at saturation, but no increase was seen after correction for mass loss. Free water content followed gravimetric moisture content in the decaying state, but the saturated state measurements revealed an initial increase and subsequent decrease with mass loss. Degradation caused changes in hygroscopicity, but our data show that overall moisture content is regulated by fungal activity rather than by material properties. These findings highlight the complexity of water interactions during fungal degradation, offering valuable new insights into wood degradation mechanisms.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"209 ","pages":"Article 106296"},"PeriodicalIF":4.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring calcium carbonate biomineralization by yeasts isolated from cement-associated environments","authors":"Giovana Manzini ,&nbsp;Giuseppe Ciaramella Moita ,&nbsp;Romildo Dias Toledo Filho ,&nbsp;Eliana Flávia Camporese Sérvulo ,&nbsp;Vitor Liduino","doi":"10.1016/j.ibiod.2025.106275","DOIUrl":"10.1016/j.ibiod.2025.106275","url":null,"abstract":"<div><div>This study advances microbial-induced calcium carbonate precipitation (MICP), a technology with potential to mitigate carbon emissions. A composite mixture of natural and recycled stone aggregates was tested for the isolation of carbonate-forming fungi, seeking to explore alternative eukaryotic microorganisms capable of MICP. Three yeast and seven mold cultures were isolated. The yeast isolates, identified through ITS sequencing as <em>Candida orthopsilosis</em>, <em>Naganishia</em> sp., and <em>Rhodotorula mucilaginosa</em>, were evaluated for their ability to produce CaCO₃ in an acetate-based medium over a 7-day cultivation period. All three yeasts generated mineral residues, showing different overall yields (ranging from 0.6 to 1.6 g) but with similar carbonate contents (∼20 % as CaCO₃). Fourier-transform infrared spectroscopy (FTIR) confirmed the presence of carbonate groups in all mineralized residues. At the same time, thermogravimetric (TG-DTG) analyses revealed that among the isolates, <em>R. mucilaginosa</em> achieved the highest conversion rate of calcium acetate to CaCO₃ (48.8 ± 3.1 %), corresponding to a precipitation yield of 0.29 g CaCO₃, more than twice that obtained for the other isolates. X-ray diffraction (XRD) analyses revealed that the only CaCO₃ polymorph formed was vaterite. These findings provide new insights into the biomineralization capacity of non-ureolytic yeasts and highlight their potential applicability as alternative biocatalysts in MICP-based technologies.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"209 ","pages":"Article 106275"},"PeriodicalIF":4.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145872364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of free and immobilised potent lipase-producing bacteria for dual fat-oil-grease (FOG) degradation and sesame butter wastewater treatment","authors":"Cathbert Nomwesigwa ,&nbsp;Mahmoud Nasr ,&nbsp;Sameh Awad ,&nbsp;Ahmed Abdel-Mawgood","doi":"10.1016/j.ibiod.2026.106278","DOIUrl":"10.1016/j.ibiod.2026.106278","url":null,"abstract":"<div><div>This study investigated the efficiency of a novel polyvinyl alcohol-sodium alginate (PVA/SA) bead system for treating sesame butter wastewater (SBW) and concurrently producing bio-alkanes. The best two isolates were identified through 16S rRNA analysis and investigated as freely suspended individuals and in consortium treatments. The effect of bacterial immobilization on SBW detoxification was then investigated. Encapsulating <em>Pseudomonas aeruginosa</em> and <em>Bacillus paramycoides</em> within a sodium alginate (SA) matrix facilitated superior mass transfer of lipid globules (oleosomes), while ensuring high cell retention and protection against a harsh environment in the degradation system. Applying the PVA-SA composite yielded excellent results in successive degradation runs, i.e., up to 4 cycles. Analytical results from gas chromatography-mass spectrometry (GC-MS) justified the activation of the fatty aldehyde decarboxylase (FadD), fatty acyl-CoA reductase (Acr), and fatty aldehyde decarbonylase (CER1) enzymatic cascade. This activity resulted in the successful transformation of acyl groups into value-added hydrocarbons, specifically hexadecane and pentane. Detecting an m/z 115 peak (sodiated glycerol) served as a distinctive molecular marker for glycerol production. This SBW bacterial degradation achieved significant reductions in fat-oil-grease (FOG) = 95.18 ± 3.81 %, chemical oxygen demand = 89.96 ± 2.70 %, and total suspended solids = 86.15 ± 3.45 %. The current results demonstrate the significance of coupling industrial wastewater remediation with the sustainable synthesis of bio-based chemicals (e.g., hexadecane, dodecane, and pentane). Additional studies should validate the <em>Pseudomonas aeruginosa</em> and <em>Bacillus paramycoides</em> performance for FOG degradation under industrial-scale applications, further encouraging the private sectors to invest in the simultaneous remediation of high-strength lipid-rich effluents and the recovery of high-value chemical precursors.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"209 ","pages":"Article 106278"},"PeriodicalIF":4.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic degradation of polycyclic aromatic hydrocarbons by Fusarium and Priestia in saline environments: From kinetic characterization to multi-omics dissection","authors":"Fenglei Han ,&nbsp;Zhoutong Liu ,&nbsp;Xiuxia Zhang ,&nbsp;Fan Yang ,&nbsp;Biao Wang ,&nbsp;Feng Yao ,&nbsp;De Li ,&nbsp;Kang Xiong ,&nbsp;Lihan Ren","doi":"10.1016/j.ibiod.2026.106279","DOIUrl":"10.1016/j.ibiod.2026.106279","url":null,"abstract":"<div><div>This study addresses the challenges of low bioavailability and restricted degradation efficiency of polycyclic aromatic hydrocarbons in saline environments by isolating the fungus <em>Fusarium</em> FG and the bacterium <em>Priestia</em> BT1 from saline contaminated soil in Dagang Oilfield, Tianjin. A fungi-bacteria FG-BT1 (FB) consortium was constructed and evaluated for its synergistic degradation performance using pyrene as a model pollutant. Under 3 % salinity and 50 mg/L pyrene, the FB consortium achieved a degradation rate of 41.98 % within 10 days, significantly higher than individual strains. The consortium also exhibited broad-spectrum effects on phenanthrene and naphthalene. Adsorption-degradation kinetics showed that the consortium followed the pseudo-second-order adsorption model and the Monod degradation model. Genomic analysis revealed complementary metabolic pathways in BT1 and FG, forming a cross-metabolic network. Untargeted metabolomics identified key products and intermediates, including 1-hydroxypyrene, 1,2-naphthoquinone, phthalic acid, and protocatechuic acid, and emphasized the role of DGTS-type biosurfactants and 12-O-D-Glucuronoside-13-Hydroxyoctadec-9Z-Enoate in enhancing pollutant solubility and transmembrane transport. Future research will focus on soil simulation remediation experiments to assess the consortium's performance in real-world conditions and explore its potential integration with existing chemical treatment technologies for more efficient and sustainable remediation strategies.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"209 ","pages":"Article 106279"},"PeriodicalIF":4.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment and cultivation of thermophilic Bathyarchaeia from oil reservoirs via systematic cultivation profiling","authors":"Qing-Ping He ,&nbsp;Yi-Fan Liu ,&nbsp;Bo-Wen Wang ,&nbsp;Lei Zhou ,&nbsp;Shi-Zhong Yang ,&nbsp;Ji-Dong Gu ,&nbsp;Bo-Zhong Mu","doi":"10.1016/j.ibiod.2025.106270","DOIUrl":"10.1016/j.ibiod.2025.106270","url":null,"abstract":"<div><div>Bathyarchaeia, among the most ubiquitous and abundant microorganisms in nature, play pivotal roles in global carbon cycling. While efforts on their enrichment, classification, genomic, and transcriptomic profiles have been extensively made, pure culture or co-culture with highly enriched <em>Bathyarchaeia</em> remains challenging, particularly in subsurface ecosystems like oil reservoirs. Here, we present a systematic workflow that integrated the Hungate roll-tube technique with 16S rRNA gene amplicon sequencing of total biomass to evaluate cultivation outcomes and identify optimal conditions for <em>Bathyarchaeia</em> by analyzing substrates and temperatures. Under cultivation conditions with various methoxylated aromatic substrates (lignin, syringaldehyde, vanillin, and 3,4,5-trimethoxybenzoate) and three temperatures, this approach led to the successful acquisition of 45 co-cultures containing <em>Bathyarchaeia</em>. Phylogenetic analysis revealed that all recovered <em>Bathyarchaeia</em> operational taxonomic units belonged to subgroup Bathy-6. Co-occurrence network analysis and cultivation results suggested mutualistic interactions between Bathy-6, specific bacterial partners (genus <em>Coprothermobacter</em>, genus <em>Desulfocurvibacter</em>, genus <em>Thermotoga</em>, genus <em>Biomaibacter</em> and genus <em>Thermodesulfovibrio</em>), and methanogens (genus <em>Methanothermobacter</em>, genus <em>Methanothrix</em> and genus <em>Ca.</em> Methanomethylicus) during the degradation of methoxylated aromatics. The optimal cultivation condition for Bathy-6 was determined to be 46 °C with syringaldehyde as the substrate. Furthermore, a novel and efficient DNA extraction protocol from gellan gum-embedded biomass was developed using sodium citrate acidification. Our study provides a effective cultivation strategy for accessing the cryptic <em>Bathyarchaeia</em> and yields a valuable repository of co-cultures. These findings significantly advance our comprehension of the metabolic capabilities and mutualistic lifestyle of <em>Bathyarchaeia</em> in the anaerobic degradation of aromatic compounds within oil reservoir ecosystems.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"208 ","pages":"Article 106270"},"PeriodicalIF":4.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and screening of acid-secreting bacterial strains isolated from limestone of the Longmen Grottoes monuments","authors":"Lu Wang ,&nbsp;Chaolong Ma ,&nbsp;Fasi Wu ,&nbsp;Xiaobo Liu","doi":"10.1016/j.ibiod.2025.106255","DOIUrl":"10.1016/j.ibiod.2025.106255","url":null,"abstract":"<div><div>Stone cultural heritage exposed to outdoor conditions often suffers from microbially derived biodeterioration processes. Although modern culture-independent approaches have expanded our understanding of the community and function of cultural heritage microbiomes, studies on the isolation and identification of microbial biodeteriogens and their biodeterioration processes affecting stone heritage are still necessary. Here, we isolated 56 bacterial strains from the deteriorated limestone monuments of the Longmen Grottoes and identified 21 potential bacterial strains with significant deteriorating capabilities through limestone dissolution laboratory tests. These bacterial strains showed their biodeteriogenic action by lowering the pH of the surrounding environment by secreting organic acids and/or sulfuric acid, which then dissolved calcium and/or magnesium carbonates of the limestone. Based on the abundance distribution in the biodeteriorated areas sampled and their biodeterioration ability in laboratory tests, three types of biodeteriogenic bacterial strains, including acidophilic (e.g., <em>Buttiauxella gaviniae</em>), methyl-oxidizing (e.g., <em>Methylorubrum populi</em>), and sulfur-oxidizing (<em>Paracoccus sulfuroxidans</em>) were identified and considered as potential representative biodeteriogens. Their biodeterioration mechanisms primarily include CaCO₃ solubilization caused by biogenic organic and inorganic acids on the limestone monuments of the Longmen Grottoes. The findings will improve our understanding of mechanisms underpinning microbial biodeterioration processes of stone monuments and expand the microbial biodeteriogen database for stone heritage conservation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"208 ","pages":"Article 106255"},"PeriodicalIF":4.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the dual effects of DOM from composted pig manure on Aliinostoc (Cyanobacteria): Convergence of positive and negative impacts","authors":"Qiong Yan,&nbsp;Congcong Wang,&nbsp;Yu Shu,&nbsp;Yuexi Jiang,&nbsp;Anwei Chen,&nbsp;Liang Peng,&nbsp;Si Luo,&nbsp;Jihai Shao","doi":"10.1016/j.ibiod.2025.106267","DOIUrl":"10.1016/j.ibiod.2025.106267","url":null,"abstract":"<div><div>The complex composition of dissolved organic matter (DOM) underlies its diverse biological effects on microorganisms. This study investigated the dual positive and negative effects of DOM derived from composted pig manure (CPM-DOM) on the diazotrophic cyanobacterium <em>Aliinostoc</em> sp. YYLX235. Physiological responses, including growth, photosynthesis, N₂-fixation, and antioxidative activity, were evaluated under CPM-DOM exposure (40–240 mg L⁻¹). After 2 days of exposure to CPM-DOM at concentrations ranging from 40 to 240 mg L⁻¹, the growth of <em>Aliinostoc</em> increased by 74.8 %–167.2 %, while its nitrogen-fixation activity was inhibited by 67.0 % and 87.4 % at CPM-DOM concentrations of 160 mg L⁻¹ and 240 mg L⁻¹, respectively. The temporal dynamics of phycobiliprotein content under CPM-DOM exposure exhibited an opposite trend to the electron transport efficiency of photosystem II (PS II). The combined effects of nutrient supplementation and allelopathy likely accounted for the complex physiological responses of <em>Aliinostoc</em> to CPM-DOM exposure. Certain toxic fractions in CPM-DOM, such as cysteinylglycine-containing substances, may bind to nitrogenase and PS II D1 protein, thereby interfering with their functions. CPM-DOM also reduced reactive oxygen species (ROS) and lipid peroxidation, indicating an absence of oxidative stress. These results reveal concentration-dependent trade-offs between growth stimulation and metabolic inhibition, with significant implications for optimizing the co-application of composted pig manure fertilizers and diazotrophic cyanobacteria-based biofertilizers in rice cultivation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"208 ","pages":"Article 106267"},"PeriodicalIF":4.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145748552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}