International Biodeterioration & Biodegradation最新文献

{"title":"Biogenic palladium nanoparticles reclaimed by a novel strain Citrobacter braakii Z1 accelerating RDX biodegradation in the munition effluent","authors":"Shichong Zhao,&nbsp;Bin Hou,&nbsp;Kunchan Zhang,&nbsp;Yating Jia,&nbsp;Jing Lu","doi":"10.1016/j.ibiod.2025.106075","DOIUrl":"10.1016/j.ibiod.2025.106075","url":null,"abstract":"<div><div>The microbial degradation of high-energy explosives has emerged as a cost-effective environmental remediation strategy, but is hindered by low degradation efficiency due to the slow electron transfer. Biogenic Pd⁰ nanoparticles (bio-Pd⁰) demonstrate dual functionality, maintaining catalytic activity for N-NO₂ bond reduction while substantially enhancing electron transfer efficiency. This study introduces an innovative single-cell system that synergistically combines chemical catalysis with biological enhancement through in situ synthesis of nanoparticles. Comprehensive characterization through electron microscopy, spectroscopic analysis, and electrochemical measurements revealed that bio-Pd⁰ synthesized by <em>Citrobacter braakii</em> Z1 (<em>C. braakii</em> Z1) were preferentially localized in the periplasmic space and extracellular matrix, forming a stable hybrid named bio-Pd⁰@<em>C. braakii</em> Z1. This process relied on the bio-reduction mediated by biohydrogen and extracellular electron transfer processes via c-type cytochromes (c-Cyts) and flavins. Notably, RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) degradation efficiency increased from 11 % with microbes alone to nearly 100 % with bio-Pd⁰. Although the observed RDX degradation in inactivated bio-Pd⁰@<em>C. braakii</em> Z1 and the detected hydrogenolysis intermediates indicated the participation of chemical catalysis, the negative correlation between RDX degradation rate with the Pd:biomass ratios suggested the biology process dominated RDX degradation. Meanwhile, the higher output current in bio-Pd⁰@<em>C. braakii</em> Z1 and the extracellular electron consumption by RDX indicated the bio-Pd⁰-mediated extracellular biodegradation was the primary driver for improved RDX removal efficiency. Toxicity evaluation confirmed that the in-situ combination of Pd-catalyzed chemical destruction and biological enhancement in a single cell is a feasible and environmentally friendly strategy for RDX removal with a reduced ecological risk.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106075"},"PeriodicalIF":4.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic stress induced by nitrite enhances biocide kill of sulfate reducing bacteria in oilfield enrichments","authors":"Danika Nicoletti ,&nbsp;Bei Yin ,&nbsp;Jordan Schmidt ,&nbsp;Kenneth Wunch ,&nbsp;Lisa Gieg ,&nbsp;Gary Jenneman","doi":"10.1016/j.ibiod.2025.106067","DOIUrl":"10.1016/j.ibiod.2025.106067","url":null,"abstract":"<div><div>Biocides are commonly employed in oilfield settings to mitigate the activity of sulfate reducing bacteria (SRB) that cause biofouling, reservoir souring and microbiologically influenced corrosion. Key challenges of applying biocides in the oilfield include their environmental toxicity and the need to apply high concentrations to overcome their deactivation and degradation by chemical and physical conditions. One strategy to reduce biocide dosages and ecotoxicity is to apply chemicals that enhance their activity. Nitrite, a known metabolic inhibitor of SRB, was previously found to be synergistic in inhibiting sulfate reduction when used in combination with glutaraldehyde. In the present study, pre-treatment of an SRB enrichment with 2.0 mM nitrite to lower cellular ATP was found to enhance the biocidal activity of glutaraldehyde. These results revealed a linear relationship exists between the decrease in adenylate energy charge of an SRB enrichment and a 10,000-fold increase in planktonic SRB kill, supporting a mechanism that metabolic stress imposed by nitrite enhances the biocidal activity of glutaraldehyde. Furthermore, the results showed increasing nitrite pre-treatment times support lower doses of glutaraldehyde needed to achieve enhanced SRB kill while a nitrite concentration of less than 0.04 mM was effective suggesting nitrite is a very efficient biocide enhancer.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106067"},"PeriodicalIF":4.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid removal of 2,4-DCP by activated carbon loaded nano-zero valence iron immobilized microorganisms","authors":"Kangning Dong ,&nbsp;Xiuxia Zhang ,&nbsp;De Li ,&nbsp;Kang Xiong ,&nbsp;Lihan Ren ,&nbsp;Mingbo Sun ,&nbsp;Zhengyong Lv","doi":"10.1016/j.ibiod.2025.106071","DOIUrl":"10.1016/j.ibiod.2025.106071","url":null,"abstract":"<div><div>PEG-nZVI/GAC@<em>B</em> was used to repair 2,4-DCP contamination which was difficult to solve by traditional physical, chemical and biological methods. Characterization techniques confirm the successful incorporation of nZVI particles and <em>Bacillus marisflavi</em> into the GAC's surface folds and pores. The polyethylene glycol 4000 (PEG-4000) coating prevents the formation of Fe³⁺ on nZVI surfaces. Compared to standard GAC, PEG-nZVI/GAC@<em>B</em> demonstrates higher electron transfer efficiency. Key parameters including nZVI content, PEG/nZVI mass ratio, and immobilization time were optimized for maximum efficiency. PEG-nZVI/GAC@<em>B</em> also shows effectiveness in removing other chlorophenol compounds. The removal process primarily involves GAC adsorption, followed by <em>Bacillus marisflavi</em> degradation and nZVI-induced reductive dechlorination. Analyses using IC and GC-MS reveal rapid dechlorination of 2,4-DCP adsorbed on immobilized microorganism by nZVI, with <em>Bacillus marisflavi</em> facilitating further mineralization of the system by degrading 2,4-DCP and its dechlorination products. Most intermediate degradation products exhibit lower ecological toxicity than 2,4-DCP, highlighting immobilized microorganism as a promising solution for 2,4-DCP pollution.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106071"},"PeriodicalIF":4.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotransformation and bioaccumulation of selenium by arbuscular mycorrhizal fungi associated with maize roots in natural seleniferous soils","authors":"Tanveer Kaur ,&nbsp;N. Tejo Prakash ,&nbsp;M. Sudhakara Reddy","doi":"10.1016/j.ibiod.2025.106068","DOIUrl":"10.1016/j.ibiod.2025.106068","url":null,"abstract":"<div><div>This study evaluated the role of arbuscular mycorrhizal fungi (AMF) isolated from naturally seleniferous soils in promoting plant growth, selenium (Se) uptake, and biotransformation in naturally selenium-contaminated fields. In AMF-inoculated plants, root biomass, shoot biomass, and maize cob biomass increased by 2.35-fold, 2.32-fold, and 1.93-fold, respectively, compared to control plants. Notably, AMF roots accumulated 7.80 % more selenium than control roots. However, selenium accumulation in the shoots and grains of AMF-inoculated plants was reduced by 71.83 % and 49.92 %, respectively. X-ray absorption near-edge spectroscopy revealed that toxic inorganic selenium species present in control plant roots were replaced by reduced elemental selenium in AMF-inoculated plants. Furthermore, AMF-inoculated plants exhibited higher levels of volatile organic selenium compounds, such as dimethyl selenide and dimethyldiselenide, which escaped from plant tissues, acting as a detoxification mechanism to mitigate selenium toxicity. To the best of our knowledge, this study is the first to report the role of indigenous AMF isolated from selenium-contaminated soils in facilitating selenium bioaccumulation and biotransformation to less toxic forms in plants under field conditions. The findings highlight that AMF not only enhance plant growth under selenium-stressed conditions by limiting selenium translocation to above-ground tissues but also protect plants by transforming selenium into methylated volatile derivatives. Thus, AMF protect the plants from Se toxicity by biotransformation to lesser toxic forms as well as enhancing plant growth activities. This approach can lead to successful utilization of seleniferous soils, which otherwise are considered not suitable for agricultural use.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106068"},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodeterioration of cultural heritage monuments: A review of their deterioration mechanisms and conservation","authors":"Sangeeta Yadav ,&nbsp;Diane Purchase","doi":"10.1016/j.ibiod.2025.106066","DOIUrl":"10.1016/j.ibiod.2025.106066","url":null,"abstract":"<div><div>Geochemical cycles result in the chemical, physical, and mineralogical modification of rocks, ultimately leading to the formation of soil. However, when stones and rocks form part of historic buildings and monuments, the effects are deleterious. In addition, microorganisms colonize these monuments over time, resulting in the formation of biofilms, while microbial metabolites cause physical weakening and discoloration of the stone. This process, known as biodeterioration, results in a significant loss of cultural heritage. Purification and 16S rDNA sequencing of bacteria growing on heritage monuments revealed that the most prominent taxa were closely related to <em>Bacillus</em> spp.<em>, Arthrobacter</em> spp., <em>Staphylococcus</em> spp. and <em>Paenibacillus</em> spp. In addition to bacteria, some fungal strains of the genera <em>Penicillium</em>, <em>Aspergillus</em>, <em>Fusarium</em> and <em>Alternaria</em> were also reported. To formulate effective conservation strategies to prevent biodeterioration and restore monuments, it is important to identify the microorganisms colonizing the substrate and the energy sources they utilize for sustenance. With this perspective, this review focuses on studies that explored the process of biodeterioration, the mechanisms by which microbes colonize and impact monuments, the techniques used to assess biodeterioration and conservation strategies designed to preserve the monuments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106066"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new control strategy of sulfate-reducing microorganisms and corrosion rate from a high-temperature oil reservoir by nitrate/nitrite coupling with nitrate-reducing bacteria","authors":"Lei Zhou ,&nbsp;Jie Gao ,&nbsp;Yu-Xuan Li ,&nbsp;Jun Wu ,&nbsp;Biao Wang ,&nbsp;Yi-Fan Liu ,&nbsp;Shi-Zhong Yang ,&nbsp;Ji-Dong Gu ,&nbsp;Bo-Zhong Mu","doi":"10.1016/j.ibiod.2025.106065","DOIUrl":"10.1016/j.ibiod.2025.106065","url":null,"abstract":"<div><div>Different water-flooded oil reservoirs shape diverse compositions of sulfate-reducing microorganisms (SRM), and the activities of key SRM may involve different metabolic, corrosion and inhibition mechanisms. However, the current bioinhibition methods for SRM in various petroleum reservoirs are complex and variable, and universal and systematic model methods are lacking for field application. In the present study, the effective inhibition of SRM from the production water of the Jiangsu Oilfield was investigated using inhibitors and nitrate-reducing bacteria (NRB). The experiments, involving different concentrations of selective inorganic inhibitors, showed that nitrate and nitrite exhibited prominent inhibitory effects on the SRM community (JS) with sulfide reduction by 49.0%–62.5%, followed by molybdate and tungstate. With the target of the key SRM (<em>Archaeoglobus</em>) in the production water of the Jiangsu Oilfield, nitrate/nitrite and three NRB strains were evaluated for their inhibition performance. The nitrate-containing treatments were more related to the NRB species, whereas the nitrite-containing treatments were more related to nitrite concentrations. Comparatively, the sulfide inhibition of the nitrite-containing treatments was more significant, with an obvious influence on enzyme contents (Apr and Dsr). Two preferred inhibition combinations were obtained: nitrate (10 mmol/L) and ZW3; nitrite (5 mmol/L) and ZW3. They have been applied in the SRM community (JS) for effectiveness evaluation, with excellent performance in the control of sulfide production and corrosion rate. A nitrate-mediated model method for the bioinhibition of SRM in oil reservoirs is proposed. These results provide insights and strategies for microbial inhibition of SRM and corrosion control in oilfields.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106065"},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the dynamic changes of physicochemical properties, nitrogen functional genes, bacterial communities and their interactions during the composting of Chinese medicinal herbal residues","authors":"Wanting Zheng ,&nbsp;Lisheng Wang ,&nbsp;Qiannuo Zeng,&nbsp;Jiayi Fu,&nbsp;Minxin Rao,&nbsp;Ying Zhang","doi":"10.1016/j.ibiod.2025.106063","DOIUrl":"10.1016/j.ibiod.2025.106063","url":null,"abstract":"<div><div>Aerobic composting of Chinese medicinal herbal residues (CMHRs) constitutes an efficacious waste management strategy, yet optimizing nitrogen content remains challenging. The variations of physicochemical properties, nitrogen functional genes, and bacterial communities throughout the composting process, along with their interrelationships were explored in this study. The final compost product achieved maturity, with a C/N ratio of 10.96 and a nitrification index of 0.19. Quantitative PCR analysis indicated suboptimal nitrification efficiency due to insufficient activity of nitrifying microorganisms. 52 biomarkers at four stages of composting were identified by linear discriminant analysis effect size. Functional annotation of prokaryotic taxa exposed chemoheterotrophy (17.05%–25.52%) and aerobic chemoheterotrophy (11.84%–22.65%) as dominant bacterial functions. Canonical correspondence analysis discovered total nitrogen exerted the most significant impact on bacterial genus distribution. Bacteria affectting nitrification genes and nitration products, especially <em>Anseongella</em> and <em>Weissella</em>, were spotted through network analysis. The results revealed the important microbial groups facilitating the nitrogen conversion of compost of CMHRs, which provided a basis for optimizing nitrogen retention and improving the quality of compost.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106063"},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of chicken feathers under oxygen-limited conditions: An alternative approach for nutrient recovery from feather waste","authors":"Hai Nguyen ,&nbsp;Hoang Le ,&nbsp;Le Nguyen ,&nbsp;Anh Do ,&nbsp;Oanh Le ,&nbsp;Hang Dinh","doi":"10.1016/j.ibiod.2025.106064","DOIUrl":"10.1016/j.ibiod.2025.106064","url":null,"abstract":"<div><div>Poultry feathers are a challenging-to-degrade solid waste that requires proper management and treatment technologies. Conventionally, feather waste is treated by composting, which requires oxygen and takes a long time. Here, we present a method for degrading feather waste under oxygen-limited conditions, shortening the treatment time to 8 weeks while achieving a high decomposition efficiency of 81 %. The emission of sulfide odor was controlled as the degradation process was performed in closed chambers without aeration. The process produced feather lysate at a high yield of 0.9 L kg⁻¹, retaining most of the nutrients from the feather waste. No PAHs, heavy metals, or pathogens were detected in the feather lysate, indicating a low risk for agricultural use. Feather lysate with a high keratinase content of 1330.13 U mL⁻¹ can be a source for obtaining this enzyme for various applications. The bacterial genera <em>Keratinibaculum</em> and <em>Bacillus</em> were identified as key drivers of the feather decomposition process.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106064"},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biodegradation pathway of HBCDs in the enriched microbial community","authors":"Bing Zhang ,&nbsp;Wenqi Luo ,&nbsp;Yongjin Liu ,&nbsp;Haonan Chen ,&nbsp;Tiantian Pan ,&nbsp;Baole Wang ,&nbsp;Keyan Li ,&nbsp;Huaqing Jia ,&nbsp;Shirou Zhou ,&nbsp;Shanshan Meng ,&nbsp;Xueying Ye ,&nbsp;Tao Peng ,&nbsp;Hui Wang ,&nbsp;Ji-Dong Gu ,&nbsp;Fei Yu ,&nbsp;Zhong Hu","doi":"10.1016/j.ibiod.2025.106061","DOIUrl":"10.1016/j.ibiod.2025.106061","url":null,"abstract":"<div><div>1,2,5,6,9,10-Hexabromocyclododecanes (HBCDs) have been widely used in industrial applications, leading to serious environmental contamination. Previously, we reported that the biotransformation of HBCDs in the enriched microbial communities might involve the interactions between degraders and other taxa. In the present study, we further investigate this phenomenon in these communities. We found that all microbial communities acquired HBCDs degradation capacity at the 24th transfer, with degradation rates ranging from 12.8% to 55.9%. The degradation of HBCDs was coupled with the growth of culturable bacteria in the communities. <em>Alcanivorax</em> (81.14%, relative abundance) maintained its dominance in the microbial community SZH2-24. Moreover, metatranscriptomic analysis revealed that <em>dadAH</em> and <em>dadBH</em> were the only dehalogenase genes induced by HBCDs. Functional genes of both <em>Alcanivorax</em> and other taxa were potentially involved in the downstream biodegradation pathway. Debrominated products were likely oxidized, hydrolyzed, and ring-cleaved by phenol 2-monooxygenase (<em>Alcanivorax</em>), toluene methyl-monooxygenase electron transfer component (<em>Hyphomicrobium</em>), quercetin 2,3-dioxygenase (<em>Hyphomicrobium</em> and <em>Alcanivorax</em>), and 4,5-DOPA dioxygenase extradiol (<em>Alcanivorax</em>). Subsequently, the ring-cleavage products were probably degraded via Fatty acid metabolism (Hyphomicrobiales, <em>Alcanivorax</em>, and <em>Hyphomicrobium</em>) rather than TCA cycle. <em>Alcanivorax</em> harbored a complete degradation pathway, which might have provided a competitive advantage over other taxa. In contrast, minor taxa might degrade downstream products.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106061"},"PeriodicalIF":4.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaerobic reductive dechlorination of chlorobenzenes by an enrichment culture containing Dehalobacter species","authors":"Shaohua Cao ,&nbsp;Dexuan Tang ,&nbsp;Yang Tian ,&nbsp;Lianghua Lu ,&nbsp;Wenjing Qiao ,&nbsp;Tao Long ,&nbsp;Jiandong Jiang","doi":"10.1016/j.ibiod.2025.106062","DOIUrl":"10.1016/j.ibiod.2025.106062","url":null,"abstract":"<div><div>Chlorinated benzenes are pervasive contaminants in soil and groundwater at industrial sites globally. We previously obtained an anaerobic 1,2,4-trichlorobenzene-dechlorinating enrichment culture originated from a chemical plant. In this study, we evaluated the dehalogenation capability of this consortium to utilize all chlorobenzene congeners as the ultimate electron acceptors. Over a period of approximately 250 days’ incubation, 11 out of 12 chlorobenzene congeners were successfully dechlorinated to monochlorobenzene, suggesting that the consortium has no preferential dehalogenation pattern for flanked chlorines on the benzene ring. Distinct dechlorination pathways strongly suggested that novel dechlorinator and reductive dehalogenases prevailed in this consortium. The 16S rRNA gene amplicon sequencing revealed that the microbial communities were predominantly composed of fermenters such as <em>Sporomusa</em>, <em>Desulfitobacterium</em> and <em>Longilinea</em>; methanogenic <em>Methanobacterium</em>; and the organohalide-respiring bacterium <em>Dehalobacter</em>. The bottles actively engaged in chlorobenzene dechlorination harbored higher abundances of <em>Dehalobacter</em> species. Quantitative PCR analysis further demonstrated that <em>Dehalobacter</em> conserved energy from chlorobenzene dechlorination, with growth yields ranging from 1.2 × 10⁵ to 2.27 × 10⁷ copies per μmol of Cl⁻ released. The versatile chlorobenzene dechlorination capacities of the consortium underscores its significant potential for remediating chlorobenzene-contaminated field sites.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106062"},"PeriodicalIF":4.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}