International Biodeterioration & Biodegradation最新文献

{"title":"Assessing the compost-ability of oil refinery sludge in the presence or absence of earthworms: An eco-friendly approach of biodegradation","authors":"Snigdha Nath ,&nbsp;Ujjal Dutta ,&nbsp;Sumpam Tangjang ,&nbsp;Subhasish Das","doi":"10.1016/j.ibiod.2024.105987","DOIUrl":"10.1016/j.ibiod.2024.105987","url":null,"abstract":"<div><div>Composting offers a low-cost and sustainable approach in waste management, however, knowledge about its feasibility in oil refinery sludge (ORS) bioconversion is unknown. We investigated the efficiency of three composting systems, viz., <em>Eisenia</em>-system (with <em>Eisenia fetida</em>), <em>Eudrilus</em>-system (with <em>Eudrilus eugeniae</em>), and non-earthworm system for ORS bioconversion. The earthworm-based systems recorded greater increment in available nutrients and reduction of heavy metals than non-earthworm system. The <em>Eudrilus</em>-system recorded an increment in total bacterial count by 10%, while P-solubilizing bacteria increased five-folds in <em>Eisenia</em>-system. Additionally, higher economic benefit was observed in terms of net present value (US $ 203.08), benefit-to-cost ratio (0.63) and pay-back time (1 year and 6 months) in the <em>Eudrilus-</em>processed ORS composting approach. The earthworm-based systems also demonstrated higher activity of urease, alkaline phosphatase and dehydrogenase. The clean index (&gt;4) and fertility index (&gt;3.1) of earthworm-sourced composts reflected their high manurial potential. The compost quality index (CQI &gt;7) indicated that presence of earthworms greatly facilitated the bioconversion of ORS due to higher humification and microbial diversity. Principal component analysis identified water holding capacity, total C, NPK, and earthworm growth as the major contributors in CQI. Moreover, the strong influence of enzymes in CQI (R² = 0.64) was corroborated by the linear regression analysis. Our findings underscore the potential of earthworm-based bio-systems in ORS-remediation, offering a viable way in its management while producing fortified organic manure.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105987"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethiprole biodegradation by Pseudomonas sp. NC1: Insights into the mechanisms and pathways","authors":"Wenjie Wei,&nbsp;Yingying Wu,&nbsp;Zekun Sha,&nbsp;Zhiqiang Lu,&nbsp;Minghua Wang","doi":"10.1016/j.ibiod.2024.105985","DOIUrl":"10.1016/j.ibiod.2024.105985","url":null,"abstract":"<div><div>Ethiprole is a widely used phenylpyrazole pesticide; however, microorganism-mediated degradation of ethiprole has not been reported. In this study, we isolated and identified a new and efficient strain, NC1, of <em>Pseudomonas putida</em>, using morphological, physiological, biochemical, and molecular methods. The strain, identified as <em>Pseudomonas putida</em>, utilizes ethiprole as a carbon source. We optimized the conditions for ethiprole degradation mediated by strain NC1 using the response surface method. Under optimal conditions (25 °C, pH 9, and 0.5% inoculation) we achieved a degradation rate of 79.7% within 24 h for a 50 mg/L ethiprole solution. A new ethiprole degradation pathway is proposed based on the main degradation products. The key oxidoreductase from the glucose–methanol–choline family, GmcF, involved in ethiprole degradation was identified through molecular cloning, and the degradation of other phenylimidazole insecticides by the new strain was verified using molecular docking. Additionally, we elucidated the mechanism underlying NC1 strain-mediated ethiprole degradation. We also examined the potential of the NC1 strain for the bioremediation of ethiprole-contaminated lettuce plants and soil. By optimizing this method, strain NC1 degraded 12.1% of 30 mg/L ethiprole in soil over 7 days. The half-life of ethiprole in treated lettuce plants with strain NC1 was reduced by 37.7% compared to the control group, indicating a significant effect of exogenous microorganisms on the elimination of ethiprole in lettuce plants. This study offers significant insights into the degradation pathways and mechanisms of ethiprole biodegradation and provided the basis for bioremediation of ethiprole.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105985"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular docking simulation of anaerobic biodegradation pathways of petroleum hydrocarbons in oil reservoir","authors":"Shiqi Wang ,&nbsp;Qichao Xie ,&nbsp;Haitong Yang ,&nbsp;Xiaodong Chen ,&nbsp;Wen Liu ,&nbsp;Congcong Li ,&nbsp;Shuoliang Wang","doi":"10.1016/j.ibiod.2024.105972","DOIUrl":"10.1016/j.ibiod.2024.105972","url":null,"abstract":"<div><div>In-depth study of the anaerobic biodegradation mechanism of petroleum hydrocarbons is of great significance for microbial oil recovery and bioremediation. This study retrieved microorganisms with genes for anaerobic hydrocarbon degradation from the PATRIC and NCBI databases, and compiled taxonomic information of core microorganisms containing multiple functional genes. Molecular docking simulations were conducted on 11 enzymes involved in the initial degradation pathways, such as fumarate addition reaction, anaerobic hydroxylation, and anaerobic carboxylation. The simulations revealed the petroleum hydrocarbon binding energy, binding affinity, and binding site within the enzyme cavity. The results indicated that the core microbial bacterial lineages with anaerobic degradation functional genes mainly included Bacillota and Proteobacteria. Enzymes in the same initial degradation pathway continuously catalysed the anaerobic degradation of petroleum hydrocarbons through synergistic action. The interaction force between catalytic enzymes and petroleum hydrocarbons was mainly hydrophobic interaction, with some π-π stacking interaction. This study conducted the first molecular simulation of anaerobic degradation of petroleum hydrocarbons, illustrating the interaction mode between petroleum hydrocarbons and enzymes, as well as the anaerobic degradation mechanism of petroleum hydrocarbons.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105972"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of benzene, toluene, ethylbenzene, xylene(s) biodegradation under anoxic conditions using response surface methodology","authors":"Gurpreet Kaur ,&nbsp;Satyam Verma ,&nbsp;Magdalena Krol ,&nbsp;Satinder Kaur Brar","doi":"10.1016/j.ibiod.2024.105973","DOIUrl":"10.1016/j.ibiod.2024.105973","url":null,"abstract":"<div><div>The biodegradation potential and metabolism of bacteria depend on the terminal electron acceptors present at contaminated sites. Due to the quick consumption of oxygen, microorganisms tend to use substitute electron acceptors such as nitrate, sulfate, manganese, and iron for biodegradation. The present study aims to investigate the effect of electron acceptors (nitrate, sulfate, and ferric ions) on BTEX biodegradation using <em>Bacillus infantis (B. infantis)</em> and <em>Microbacterium esteraromaticum (M. esteraromaticum)</em>. The experiment was designed with response surface methodology using the Box-Behnken method. All four compounds of BTEX biodegraded with removal efficiencies ranging from 46% to 57% in Bacillus-treated samples, while 88–98% biodegradation in Microbacterium-treated cultures. The optimal growth of <em>B. infantis</em> was observed at 250 mg/L of nitrate and iron, while no effect of sulfate was observed. For <em>M. esteraromaticum</em>, 250 mg/L of nitrate and sulfate showed the maximum growth of more than 1 optical density (OD), however, no change in growth was noticed with iron treatment. The investigation showed a maximum BTEX biodegradation of 57% by <em>B. infantis</em> under sulfate reduction and overall, 98% by <em>M. esteraromaticum</em> in combined nitrate and sulfate reduction. The present work provides new insights into soil microbial community responses to electron acceptors under anoxic conditions, signifying that intrinsic microorganisms could be successfully stimulated for ISB with electron acceptors as a supplement.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105973"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Niche differentiation of denitrifying anaerobic methane oxidation bacteria and archaea in the permafrost peatlands","authors":"Lingyu Fu ,&nbsp;Xiangwen Wu ,&nbsp;Dalong Ma ,&nbsp;Weiping Yin ,&nbsp;Anwen Liu ,&nbsp;Xu Wang","doi":"10.1016/j.ibiod.2024.105990","DOIUrl":"10.1016/j.ibiod.2024.105990","url":null,"abstract":"<div><div>The anaerobic oxidation of methane (AOM) coupled to either nitrite or nitrate reduction (nitrite or nitrate-DAMO) is a process connecting global nitrogen and carbon cycles. Permafrost peatlands are important natural sources of methane, and climate warming is accelerating permafrost thaw, resulting in changes in water table and vegetation communities that are dramatically reshaping microbial-mediated methane oxidation processes, potentially creating strong positive peatland-climate feedbacks, while the ecology of DAMO bacteria and archaea in peatland soils is poorly understood. Herein, the diversity, abundance, phylogeny, and potential activity of DAMO bacteria and archaea were explored using molecular techniques and stable isotope tracing in three typical peatlands of the Greater Khingan Mountains permafrost regions. The results revealed the co-existence of DAMO bacteria and archaea, with notable variations in community structures across different peatlands, while the vertical distribution within soil profiles remained relatively stable. These variations were mainly affected by factors such as water content, total organic carbon, nitrite, and nitrate in soil. The potential activity and abundance suggested that DAMO bacteria were predominantly found in the middle soil layers, whereas DAMO archaea were more abundant in the bottom layers. Furthermore, the diversity, potential activity, and abundance of DAMO bacteria generally declined along the forest-peatland ecotone, whereas DAMO archaea exhibited an increasing trend. Partial least squares path modeling (PLS-PM) and correlation analyses revealed strong associations between DAMO activities and the abundances of the <em>pmoA</em> and <em>mcrA</em> genes, in addition to substrate availability. The relative contribution of nitrite-DAMO to the total CH₄ oxidation was 16.77%, slightly higher than that of nitrate-DAMO at 13.23%, with both contributing more significantly than AOM coupled to iron oxide reduction (Fe-AOM) at 8.65%, demonstrating that AOM are important processes for mitigating CH₄ emissions in peatlands. This research contributes to a better understanding of the biogeochemical cycling of CH₄ in permafrost peatlands and broaden our insight into the environmental significance of DAMO microorganisms.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105990"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into mechanism of 1-hydroxy-2-naphthoic acid accumulation during phenanthrene degradation by Stutzerimonas frequens SL-6","authors":"Liwei Guo ,&nbsp;Xinyu Zhang ,&nbsp;Qiang Hou ,&nbsp;Hailei Wei ,&nbsp;Yilin Gu ,&nbsp;Baisuo Zhao ,&nbsp;Haisheng Wang","doi":"10.1016/j.ibiod.2024.105975","DOIUrl":"10.1016/j.ibiod.2024.105975","url":null,"abstract":"<div><div>Oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) represent intermediate metabolites accumulating during the degradation of polycyclic aromatic hydrocarbons (PAHs), posing challenges to effective remediation of PAH contamination. Notably, 1-hydroxy-2-naphthoic acid (1H2N), distinguished by its heightened toxicity, emerges as a pivotal model compound for investigating oxy-PAHs. This research endeavors to unravel the mechanism behind the accumulation of 1H2N by <em>Stutzerimonas frequens</em> SL-6, an efficient phenanthrene-degrading bacterium. Genomic analysis of <em>S. frequens</em> SL-6 implicated <em>nahA, salA,</em> and <em>nahR</em> in the accumulation process of 1H2N. This hypothesis was validated through <em>in vitro</em> assessments of enzyme catalytic activity, reinforced by <em>in vivo</em> gene knockout and complementation studies. Our findings pinpoint SalA as a critical factor in the accumulation of 1H2N. Subsequently, the overexpression of SalA in <em>S. frequens</em> SL-6 led to a 70% reduction in the accumulation of 1H2N. These insights deepen the global understanding of microbial PAHs degradation processes and provide a theoretical foundation for the development of microbial strains capable of efficiently and thoroughly degrading PAHs.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105975"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome and transcriptome analysis of the lignite-degrading Trichoderma cf. simile WF8 strain highlights potential degradation mechanisms","authors":"Jinghua Yao ,&nbsp;Yajuan Chen ,&nbsp;Deyu Zhuo ,&nbsp;Siqiao Chen ,&nbsp;Baichao Xu ,&nbsp;Congwei Yan ,&nbsp;Wanrong Li ,&nbsp;Hui Feng ,&nbsp;Sheng Deng ,&nbsp;Feng M. Cai ,&nbsp;Andrei S. Steindorff ,&nbsp;Irina S. Druzhinina ,&nbsp;Lei Xiao ,&nbsp;Lihui Wei ,&nbsp;Paul Daly","doi":"10.1016/j.ibiod.2025.105997","DOIUrl":"10.1016/j.ibiod.2025.105997","url":null,"abstract":"<div><div>The biodegradation of lignite (brown coal) by microorganisms has the potential for bioremediation of contaminated mining sites and to generate alternative ways to valorize lignite, such as by producing humic acids or building block chemicals. Previously, a lignite-degrading strain of <em>Trichoderma</em> was isolated, but the genomic and transcriptomic basis of its lignite-degrading ability remained unknown. Here we report that the sequenced genome of the <em>T.</em> cf. <em>simile</em> WF8 strain encoded for enzymes with roles in the degradation of lignite, and potentially tolerance to lignite-breakdown products. There was only a small number of annotated unique genes in the <em>T.</em> cf. <em>simile</em> WF8 genome compared to other fungi, and likely the expression of gene families shared with other fungi is a key factor in lignite biosolubilization by <em>T.</em> cf. <em>simile</em>. The transcriptomes were analyzed of <em>T.</em> cf. <em>simile</em> cultured at two time-points with the lignite-breakdown model compounds 4-phenoxybenzoic acid (which was growth inhibitory), and phenetole and 9-10-dibutoxyanthracene (neither of which inhibited growth), and showed ∼20% of genes up-regulated by one or more of these compounds. The analysis highlights candidates for characterization and engineering enzyme over-expressing <em>T.</em> cf. <em>simile</em> strains with potentially improved degradation capacity, <em>e.g</em>., laccases and peroxidases, or tolerance and catabolism of breakdown products, <em>e.g.</em>, cytochrome P450s, and ring cleavage dioxygenases.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105997"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignite degraded by Trichoderma citrinoviride: Products, processes and mechanisms","authors":"Xingyu Mu ,&nbsp;Meitong Li ,&nbsp;Jianhua Tian ,&nbsp;Wenjiao Yuan ,&nbsp;Hui Shen ,&nbsp;Ruijie Zhang ,&nbsp;Shuwei Li ,&nbsp;Xin Feng ,&nbsp;Xuening Fei ,&nbsp;Yuhong Xie","doi":"10.1016/j.ibiod.2024.105957","DOIUrl":"10.1016/j.ibiod.2024.105957","url":null,"abstract":"<div><div>The biodegradation of lignite facilitates the efficient utilization of low-rank coal, and its degradation products, processes, and mechanisms are important areas of research. In this study, the biodegradation process of the lignite was analyzed using scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Firstly, the structural changes of lignite caused by <em>Trichoderma citrinoviride</em> were analyzed. Secondly, the main product detected by GC-MS from the degradation of lignite by <em>Trichoderma citrinoviride</em> was identified as erucylamide, with a conversion rate of 12.23%. The main product that was volatilized at 280 °C during the degradation of lignite by <em>Trichoderma citrinoviride</em> was first identified by GC-MS. Finally, the oxidase, LiP, MnP, Lac, and PPO secreted by <em>Trichoderma citrinoviride</em> were the main factors contributing to the degradation of lignite, and the degradation processes and mechanisms were inferred based on enzyme activities. This study provides fundamental experiments and data support for research on lignite in the field of the biochemical industry.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"197 ","pages":"Article 105957"},"PeriodicalIF":4.1,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifouling activity exhibited by pyrrolo compound isolated from marine sponge associated bacterium Halobacillus kuroshimensis SNSAB01 against barnacles","authors":"S. Nalini ,&nbsp;S. Venkatnarayanan ,&nbsp;D. Inbakandan ,&nbsp;Clarita Clements ,&nbsp;S.U. Mohammed Riyaz ,&nbsp;T. Stalin Dhas ,&nbsp;P. Sriyutha Murthy ,&nbsp;G. Dharani","doi":"10.1016/j.ibiod.2024.105960","DOIUrl":"10.1016/j.ibiod.2024.105960","url":null,"abstract":"<div><div>The increasing use of natural products from marine organisms and their synthetic analogs as antifouling agents is replacing chemical biocides due to their ecological compatibility. This study evaluated the antifouling potential of pyrrolo compound (PC) from the sponge-associated bacterium <em>Halobacillus kuroshimensis</em> against the barnacle <em>Amphibalanus reticulatus</em> larval survival, metamorphosis and settlement in the laboratory. The successful concentrations were also subjected to field based static immersion tests to study the efficacy of the PC extract on macrofouling settlement. Results showed decreased naupliar survival and affected larval metamorphosis in a dose-dependent manner, with varying sensitivity among developmental stages. Higher PC concentrations significantly reduced cyprid yield and settlement, with over 50% of cyprid larvae failing to settle at concentrations of 1300 μg/ml and above. Field experiments confirmed a steady decline in barnacle settlement on PC-treated coupons, with less than 50% settlement observed at concentrations of 500–2000 μg/ml over 50 days. The LC₅₀/EC₅₀ ratio for nauplius N-II was 1.11, with higher ratios observed in later stages and for the cypris (1.66), suggesting moderate toxicity and a narrow margin of safety. These ratios suggest barnacle settlement inhibition occurring through a toxic mechanism. The results demonstrate the compound's ability to disrupt barnacle settlement, but also raise concerns about its environmental impact (long-term usage), particularly the potential risks to non-target species before using the compound in real-world antifouling applications. Despite higher concentrations used compared to previous reports, PC shows potential as an effective natural antifouling compound.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"197 ","pages":"Article 105960"},"PeriodicalIF":4.1,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and chemical markers associated with biodeterioration agents isolated from archive audio-visual materials","authors":"Tereza Branysova ,&nbsp;Michał Komar ,&nbsp;Justyna Szulc ,&nbsp;Marcin Sypka ,&nbsp;Joanna Nizioł ,&nbsp;Aneta Płaza-Altamer ,&nbsp;Teresa Stryszewska ,&nbsp;Konrad Olejnik ,&nbsp;Tomasz Ruman ,&nbsp;Beata Gutarowska","doi":"10.1016/j.ibiod.2024.105959","DOIUrl":"10.1016/j.ibiod.2024.105959","url":null,"abstract":"<div><div>Biodeterioration of cultural heritage objects, including audio-visual materials, is driven by complex microbial metabolic processes that remain incompletely understood. This research addresses this gap through a model study using bacterial (<em>Bacillus</em> spp.) and fungal (<em>Alternaria</em>, <em>Aspergillus</em>, and <em>Penicillium</em> spp.) isolates on archival photographs with different binders—albumen, collodion, and gelatine. We examined the enzymatic activities, metabolomic profiles, and structural impacts of microbial colonisation over a two-month period. Isolates demonstrated specific degradation capabilities, with proteolytic and cellulolytic activities prominently observed. Metabolomic analysis revealed a significant production of carboxylic acids and organooxygen compounds across all isolates and binders, indicating common biodegradation pathways involving the breakdown of complex molecules such as cellulose. Gelatine emerged as the most conducive substrate for microbial growth and metabolic activity, exhibiting distinct metabolic profiles compared to collodion and albumen. SEM analysis uncovered preferential colonisation patterns, notably the preference of the <em>Alternaria</em> genus for collodion. The microbial activity resulted in measurable changes in pH, colour, and mechanical properties of the materials, with albumen elongation drastically reduced to 0.76%. Our findings elucidate the microbial metabolic processes driving the biodeterioration of photographic materials, providing crucial insights for developing targeted preservation strategies to safeguard audio-visual cultural heritage.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"197 ","pages":"Article 105959"},"PeriodicalIF":4.1,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}