Minghuang Ling , Ping Zheng , Xiaomei Huang , Gaili Fan , Huafeng Zhang , Zhijie Xu , Peiwen Zhuang , Changyou Wang , Hans-Peter Grossart , Kai Zhang , Zhuhua Luo
{"title":"Improving bioavailability of lignocellulosic biomass by pretreatment with the marine fungus Chaetomium sp. CS1","authors":"Minghuang Ling , Ping Zheng , Xiaomei Huang , Gaili Fan , Huafeng Zhang , Zhijie Xu , Peiwen Zhuang , Changyou Wang , Hans-Peter Grossart , Kai Zhang , Zhuhua Luo","doi":"10.1016/j.ibiod.2025.106031","DOIUrl":"10.1016/j.ibiod.2025.106031","url":null,"abstract":"<div><div>Crop straw and waste wood are abundant renewable biomass resources, but their complex lignocellulose composition limits industrial use. This study introduces an eco-friendly solution by bio-converting lignocellulosic biomass into biofuels and biochemicals using <em>Chaetomium</em> sp. CS1, a deep-sea fungus that degrades 55% of alkali lignin in 10 days. Gas chromatography-mass spectrometry (GC-MS) analysis indicated guaiacol as the primary pyrolysis product from G-type lignin, which could be further converted into alkanes and other intermediates. Transcriptomic analysis identified numerous genes encoding ligninolytic enzymes, including highly induced AA3 family enzymes with oxidoreductase and monooxygenase activities, during lignin degradation. A copper-containing nitrite reductase gene was significantly induced, enhancing denitrification and reducing nitrite to aid detoxification. Fungal pretreatment significantly improved the digestibility of sawdust by black soldier fly larvae, enhancing the bioavailability of lignocellulosic materials. These knowledge into fungal lignin degradation provide a basis for efficient lignocellulose pretreatment, enabling high-value biofuel and bioproduct production.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"199 ","pages":"Article 106031"},"PeriodicalIF":4.1,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dikra Hamadouche , Adama Zan Diarra , Fabien Fohrer , Jean-Michel Bérenger , Ahmed Benakhla , Lionel Almeras , Philippe Parola
{"title":"Assessment of MALDI-TOF MS for the identification of cultural heritage insect pests","authors":"Dikra Hamadouche , Adama Zan Diarra , Fabien Fohrer , Jean-Michel Bérenger , Ahmed Benakhla , Lionel Almeras , Philippe Parola","doi":"10.1016/j.ibiod.2025.106033","DOIUrl":"10.1016/j.ibiod.2025.106033","url":null,"abstract":"<div><div>When heritage properties and contents are threatened by various harmful insects, especially coleopterans, rapid and reliable pest identification is required for effective management. While traditional methods have limitations, MALDI-TOF MS has recently emerged as a groundbreaking identification tool. In this study, our aim was to assess the effectiveness of MALDI-TOF MS in identifying cultural heritage insect pests belonging to the Coleoptera order. To do so, adult, larva, and exuviae from eight species of Coleoptera (<em>Lasioderma serricorne</em> (<em>n</em> = 92), <em>Attagenus smirnovi</em> (<em>n</em> = 87), <em>Trogoderma versicolor</em> (<em>n</em> = 97), <em>Reesa vespulae</em> (<em>n</em> = 74), <em>Anthrenus verbasci</em> (<em>n</em> = 68), <em>Anthrenus pimpinellae</em> (<em>n</em> = 34), <em>Anthrenus flavipes</em> (<em>n</em> = 30), and <em>Thylodrias contractus</em> (<em>n</em> = 19), were subjected to MALDI-TOF MS after optimisation of the sample preparation parameters. This optimisation was based on three key parameters influencing spectrum quality: the choice of body part, the buffer volume for protein extraction, and the method and duration of sample homogenisation. Reproducible intra-species MS spectra for each developmental stage were obtained. The species specificity of MS spectra for each developmental stage supported the use of MALDI- TOF MS for specimen classification. Spectra from 68 samples, including four specimens per species and stage, were added to our homemade reference arthropod MS database (DB). Among spectra classified as being consistent, 97.10% (335/345) of the samples were correctly classified with a relevant identification score. The morphological identification of all adult specimens in the reference MS spectra DB was confirmed by molecular biology, and the sequences were deposited in GenBank. This study is the first demonstration of the effective application of MALDI-TOF MS as a reliable tool for identifying various developmental stages of Coleoptera pests which pose a threat to the cultural heritage of France.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"199 ","pages":"Article 106033"},"PeriodicalIF":4.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Baseline characteristics of the microbial community structure and composition of the world cultural heritage sites in Macau","authors":"Shanshan Meng , Youfen Qian , Pengfei Hu , Guang Huang , Ji-Dong Gu","doi":"10.1016/j.ibiod.2025.106032","DOIUrl":"10.1016/j.ibiod.2025.106032","url":null,"abstract":"<div><div>Microorganisms contribute to the deterioration of materials. In this study, samples were collected from the surfaces of different cultural heritage sites in Macau to reveal the structure and composition of microbial communities and to understand the deterioration mechanisms, which will be helpful in developing strategies to manage and protect these sites. Structure and composition of microbial communities on the surfaces of different types of cultural heritage in Macau, including ceramic-tile walls, architectures in park, and historic buildings, were analyzed by conducting 16S rRNA gene sequencing. Cyanobacteria, Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, and Bacteroidetes were found to be most abundant microbial phyla on the surfaces of these cultural heritages of Macau under subtropical conditions. Among them, Cyanobacteria was observed as the dominant microbial phylum, indicating its potential role as a key microbial group responsible for the deterioration of cultural heritages. Significant variations were also observed in the distribution of microbial groups among different surface samples. Shannon index values revealed that microbial communities on the walls of historic buildings were more diverse than those on stones and ceramic tiles. Additionally, the distribution of microorganisms within Macau's cultural heritage sites was intricately linked to temperature, humidity, and climate. The results emphasize the importance of identifying the composition of surface microbial communities for a better understanding of biodeterioration process to protect and manage the cultural heritage sites.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"199 ","pages":"Article 106032"},"PeriodicalIF":4.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Viral communities on the sandstone monuments of Angkor Wat, Bayon and Preah Vihear in Cambodia","authors":"Pengfei Hu , Xinghua Ding , Yoko Katayama , Ji-Dong Gu","doi":"10.1016/j.ibiod.2025.106024","DOIUrl":"10.1016/j.ibiod.2025.106024","url":null,"abstract":"<div><div>Viruses are ubiquitous and ecologically important, but fewer information is available for heritage. In this report, we analyzed the taxonomy and metabolic potentials of viruses on three globally renowned monuments, namely Angkor Wat (AW), Bayon Temple (Bayon Temple Entrance, BTE), Bayon temple Gallery No. 25 (BayonG25), and Preah Vihear (PV) in Cambodia by metagenomic sequencing and analysis. Virus species characterization results show that vast majority of them on these stone cultural heritage are unclassified, with a tiny small fraction affiliated with <em>Siphoviridea</em>. Results of auxiliary metabolic genes (AMGs) analyses revealed a wide range of metabolic potentials of these viruses, with cofactor and vitamin metabolism being detected at all sampling sites, suggesting that viruses may play an important role in the microbial biofilm formation, development and energy metabolism.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"199 ","pages":"Article 106024"},"PeriodicalIF":4.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yumeng Fu , Shuaishuai Hu , Jiankun Wang , Xiaoling Wang
{"title":"Competitive growth of Bacillus subtilis biofilms","authors":"Yumeng Fu , Shuaishuai Hu , Jiankun Wang , Xiaoling Wang","doi":"10.1016/j.ibiod.2025.106027","DOIUrl":"10.1016/j.ibiod.2025.106027","url":null,"abstract":"<div><div>Competition for existence exists everywhere in nature. Competition includes competition between different species and competition between the same species. In this paper, <em>Bacillus subtilis</em> which is cultured on 1.5 wt% agar gel containing minimal salts glutamate glycerol (MSgg) medium is taken as the research object to explore the competitive behavior of the same <em>Bacillus subtilis</em> in the process of biofilm formation. Wide-field fluorescence microscopy is used to observe the competition of biofilms, which helps us obtain the evolvement of the edge features of the biofilm in the competition region and the distribution of different phenotypes. We find that the premature formation of the chain structure of the matrix producing cells in the competitive region restrict the outward expansion of the motile cells and thus result in the unique competition between the biofilms of <em>Bacillus subtilis</em>. Based on the microscopic experimental analysis of cell population collective motion during the biofilm growth, a cellular automata model of biofilm growth and evolvement is constructed. We simulate different behaviors of biofilm competition by adjusting the inoculation distance and parameters characterizing environmental pressure. The results show that the competition phenomenon of biofilm is due to the fact that matrix producing cells appear in the competition area in advance and form a barrier structure to prevent the movement and expansion of motile cells to the competition area.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"199 ","pages":"Article 106027"},"PeriodicalIF":4.1,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amada El-Sabeh, Andreea-Mihaela Mlesnita, Marius Mihasan
{"title":"Integrated transcriptomic and proteomic analysis of nicotine metabolism in Paenarthrobacter nicotinovorans ATCC 49919","authors":"Amada El-Sabeh, Andreea-Mihaela Mlesnita, Marius Mihasan","doi":"10.1016/j.ibiod.2025.106017","DOIUrl":"10.1016/j.ibiod.2025.106017","url":null,"abstract":"<div><div>The global tobacco industry produces significant amounts of nicotine-containing wastes, being regarded as a major environmental threat. The appropriate treatment of nicotine-contaminated waste is required for the removal of the toxic alkaloid before safe disposal. In this context, nicotine-degrading microorganisms and their enzymatic apparatus are of major interest for decontaminating and repurposing nicotine waste. Here, by combining long-read direct RNA sequencing data and nanoLC-MS/MS based proteomics data, we focused on the multiomic characterisation of the bacterial nicotine catabolic process from <em>Paenarthrobacter nicotinovorans</em> ATCC 49919. The nicotine-related expression of 25 annotated <em>nic</em>-genes and proteins was confirmed, eight of these genes (<em>JMY29_20530</em>, <em>JMY29_20550</em>, <em>perm</em>, <em>coxF</em>, <em>coxE</em>, <em>JMY29_20640</em>, <em>JMY29_20655</em>, <em>modB</em>) being reported here first as having nicotine-related expression. Insights regarding the active mechanisms involved in integrating the nicotine catabolic pathway with the general metabolism of the bacterial cell and the defence systems employed against the oxidative stress generated during nicotine degradation are also presented. This study provides the first multiomic investigation of <em>P. nicotinovorans</em> ATCC 49919 and, moreover, the first multiomic assessment of a bacterial nicotine catabolic pathway.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"199 ","pages":"Article 106017"},"PeriodicalIF":4.1,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chunyu Fan , Xiaofang Yu , Liu Yang , Linjie Yue , Xiaoxuan Zeng , Yujia Liu , Xue Xiao , Lijuan Yang , Fuwen Luo
{"title":"Enhancing cadmium tolerance and photosynthesis in Cosmos bipinnatus Cav. By hormone methyl jasmonate","authors":"Chunyu Fan , Xiaofang Yu , Liu Yang , Linjie Yue , Xiaoxuan Zeng , Yujia Liu , Xue Xiao , Lijuan Yang , Fuwen Luo","doi":"10.1016/j.ibiod.2024.105976","DOIUrl":"10.1016/j.ibiod.2024.105976","url":null,"abstract":"<div><div><em>Cosmos bipinnatus</em> Cav. (<em>C. bipinnatus</em>), a highly cadmium (Cd)-tolerant landscape plant, shows promise for Cd pollution remediation. This study investigates the role of methyl jasmonate (MeJA) in enhancing Cd tolerance under high Cd stress (40, 120 μM) by regulating endogenous hormones. Cd stress severely impacts <em>C. bipinnatus</em>, reducing fresh/dry weight by 44.49%–83.81%, alongside declines in chlorophyll content and photosynthetic efficiency (F<sub>v</sub>/F<sub>m</sub>). However, low-concentration MeJA (0.1 μM) improves Cd tolerance by reducing water content by 3.09%–22.43%, increasing biomass by 12.82%–52.73%, decreasing Cd uptake by 4.56%–5.64%, and restoring chlorophyll synthesis and F<sub>v</sub>/F<sub>m</sub>. MeJA also modulates non-protein thiols (NPTs), especially decreasing NPTs in leaves under Cd stress and enhances glutathione conversion to phytochelatins, critical for detoxification. Endogenously, MeJA triggers rapid accumulation of jasmonic acid, abscisic acid (ABA), and indole-3-acetic acid while reducing gibberellin, which can be harmful to photosynthesis under Cd stress. MeJA also prevents prolonged ABA accumulation, mitigating its levels, thus protecting the photosynthetic system. These findings highlight MeJA's regulatory effects on hormone balance and suggest new approaches for effective soil Cd pollution remediation through enhanced plant tolerance mechanisms.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105976"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shiv Vendra Singh , Shivangi Raghuvanshi , Yogeshwar Singh , Krishna Kumar Yadav , Amel Gacem , Tony Manoj K. Nandipamu , Mohammad Khalid , Rashida Hameed , Rashmi Sharma , Debarati Datta , Saurabh Ghosh , Arpna Kumari , Ajay Kumar Singh , Biswajit Pramanick , Xiuxiu Zhang , Chongqing Wang , Maha A. Alreshidi
{"title":"Biochar's multifaceted role in bioremediation of emerging contaminants and heavy metals in complex rhizospheric ecosystem","authors":"Shiv Vendra Singh , Shivangi Raghuvanshi , Yogeshwar Singh , Krishna Kumar Yadav , Amel Gacem , Tony Manoj K. Nandipamu , Mohammad Khalid , Rashida Hameed , Rashmi Sharma , Debarati Datta , Saurabh Ghosh , Arpna Kumari , Ajay Kumar Singh , Biswajit Pramanick , Xiuxiu Zhang , Chongqing Wang , Maha A. Alreshidi","doi":"10.1016/j.ibiod.2025.106005","DOIUrl":"10.1016/j.ibiod.2025.106005","url":null,"abstract":"<div><div>Rising prevalence of emerging contaminants (ECs) and priority heavy metals (PHMs) poses grave threats to the health of the environment and humankind, majorly resulting from human activity such as mining, disposal of industrial wastes, and use of chemicals. These pollutants drastically reduce soil biodiversity, fertility, and crop yield, rendering agricultural goods hazardous. Biochar has recently received attention as a sustainable bioremediation solution for ECs and PHMs through diverse physical, chemical, and biological processes. Biochar has demonstrated significant bioremediation efficiency for PAHs, antibiotics, microplastics, and pesticides varied from 50 to 95% and 60–90% for PHMs in a wide range of ecosystems. The interactive mechanisms of complexation, precipitation, ion exchange, surface sorption, and electrostatic interaction, hydrophobic interaction electron donor and acceptor interaction altogether enhance contaminant immobilization and biodegradation. Furthermore, biochar has been shown to aid in the breakdown of contaminants while lowering the transportation and accessibility of heavy metals. Besides remediation, biochar improves the rhizospheric environment by enhancing plant growth, nutrient uptake, and soil vitality. Its ability to remove both heavy metals and organic pollutants from wastewater and soil matrices, and its influence on their bioavailability and transport, show the dual nature of biochar in restoring environments. This manuscript attempts to provide in-depth insight into the challenges that ECs and PHMs pose, the role of biochar in their removal, and delicate soil-plant-biochar interactions. The work here discusses these interacting effects, thus giving insight into the potential of biochar in the immobilization of ECs and PHMs through many interspecific reactions, and also the soil-plant-biochar interactions and possibilities for successful remediation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106005"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junxian Li , Zhongming Zheng , Yangcai Wang , Betina Lukwambe , Zhao Yang
{"title":"Nutrient removal performance and microbial community analysis in an integrated wastewater treatment system with macroalgae (U. meridionalis) and Bacillus","authors":"Junxian Li , Zhongming Zheng , Yangcai Wang , Betina Lukwambe , Zhao Yang","doi":"10.1016/j.ibiod.2025.106006","DOIUrl":"10.1016/j.ibiod.2025.106006","url":null,"abstract":"<div><div>The integrated aquaculture wastewater treatment system has been widely applied and typically consists of multiple units, among which macroalgae are critical for nutrients removal. This study investigated the impact of the addition of <em>Bacillus</em> to the macroalgae unit on the nutrient removal performance of the system and its mechanisms by analyzing the bacterial community that was identified through high-throughput sequencing of 16S rRNA. The results showed that <em>Bacillus</em> significantly influenced the structural composition of the bacterial communities both in the water and on the algal surface within the macroalgae unit. The enrichment of bacteria promoted nitrogen cycling and algal growth, such as <em>Algoriphagus</em> and <em>Marivita</em>. Futhermore,a higher average variation degree was observed, suggesting forming a more stable bacterial community. The removal rates of nitrogenous nutrients, such as TAN, NO<sub>3</sub><sup>−</sup>-N, and NO<sub>2</sub><sup>−</sup>-N, improved, reaching 99.92%, 99.62%, and 99.70%, respectively. Additionally, the biomass of the algae (<em>U. meridionalis</em>) significantly improved, reaching 157%. The <em>Bacillus</em> also increased the contribution of stochastic processes (drift) in the assembly of the bacterial communities in both the water and on the algal surface. This study provides foundational support for optimizing integrated wastewater treatment systems.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106006"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L.S. Martin , S.M. Cragg , K.W. Kot , J.R. Shipway
{"title":"Streamlining automation in a rapid wood durability test against the marine wood boring crustacean, Limnoria quadripunctata.","authors":"L.S. Martin , S.M. Cragg , K.W. Kot , J.R. Shipway","doi":"10.1016/j.ibiod.2024.105993","DOIUrl":"10.1016/j.ibiod.2024.105993","url":null,"abstract":"<div><div>Gribble significantly impact the biodegradation of wood in marine environments, causing costly damages, but can be used to easily assess wood consumption rates. Faecal pellet production indicates feeding rate and is a good proxy for degradation. However, counting large numbers of pellets by eye may be impractical with multiple replicates. Optimising a rapid, cost-effective, and user-friendly protocol to evaluate wood durability against gribble can be used in development of sustainable wood protection methods. Automated counting methods ImageJ and FlowCAM, were evaluated against manual counts for efficiency and accuracy. ImageJ was more suitable for large sample volumes, offering a balance of cost, time, and accuracy. Both methods showed greater degrees of error as faecal pellet counts increased. ImageJ tended to undercount, whereas FlowCAM often overcounted significantly. The FlowCAM rapidly processed individual samples, however loading/unloading samples increased time-consumption as replicates increased. ImageJ was able to process multiple samples at once so total time was not influenced by sample size. This research underlines the importance of optimising counting methods to accurately assess the impact of gribble on wood degradation, offering a streamlined approach for both specialist and non-specialist laboratories to evaluate wood durability and enhance the development of non-toxic, sustainable wood protection solutions.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105993"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}