International Biodeterioration & Biodegradation最新文献

{"title":"Evaluation of Trichoderma atroviride G79/11 growth intensity and metabolic activity under different additives using Biolog system","authors":"Mohsin Ali,&nbsp;Justyna Cybulska,&nbsp;Artur Zdunek,&nbsp;Magdalena Frąc","doi":"10.1016/j.ibiod.2025.106104","DOIUrl":"10.1016/j.ibiod.2025.106104","url":null,"abstract":"<div><div><em>Trichoderma</em> species have the ability to act as a safe and sustainable environmental way to enhance crop productivity and deal with pathogenic diversity. It is a crucial task to evaluate and boost their growth intensity and metabolic activity through an efficient and accurate method with novel nutritional sources. In the current study, Biolog MT2 microplates were used to evaluate the <em>T</em>. <em>atroviride</em> G79/11 growth intensity and metabolic activity in the presence of polysaccharide additives such as pectin fractions (water-soluble pectin-WSP, diluted alkali-soluble pectin-DASP, and oxalate soluble pectin-OSP) and bacterial cellulose (BC), fortified with inorganic salts. Pectin, as a significant component of fruit and vegetable industry waste, and bacterial cellulose, which has an exceptional water retention capacity, were used for the first time to stimulate the growth of <em>T. atroviride</em> G79/11. The highest metabolic efficiency of <em>T. artoviride</em> G79/11 was observed in WSP and OSP fractions supplemented with Ca, Zn, Mg and Fe. Glucose was recorded as a dominant constituent of BC composition, whereas galacturonic acid was detected with the highest amount of 84.53 %, 7.56 %, and 71.70 % of OSP, WSP, and DASP fractions, respectively. Applied polysaccharide solutions significantly differed in viscosity, which was also influenced by the sterilization process. The strain showed significantly high growth intensity and metabolic activity in potato dextrose broth amended with WSP, BC, ZnCl₂, saccharose, glucose, starch, and a combination of OSP with CaCl₂ additive as compared to its respective groups and combinations as well as FF-IF. This study emphasizes that natural polysaccharides and their possible composites can boost the growth intensity and metabolic activities of the fungal strain.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106104"},"PeriodicalIF":4.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The degradation of nicotinic acid is controlled by MarR-family transcriptional regulator NicR in Alcaligenes faecalis","authors":"Siqiong Xu ,&nbsp;Cuiwei Chu ,&nbsp;Xinyang Li ,&nbsp;Yongli Wang ,&nbsp;Hongfei Zhang ,&nbsp;Lili Li ,&nbsp;Lixia Zhu ,&nbsp;Yongchuang Liu ,&nbsp;Shuhua Fan ,&nbsp;Yuehui Tang ,&nbsp;Keshi Ma","doi":"10.1016/j.ibiod.2025.106103","DOIUrl":"10.1016/j.ibiod.2025.106103","url":null,"abstract":"<div><div>Nicotinic acid (NA), or vitamin B3, is a natural pyridine carboxylic derivative, a precursor for numerous essential biological molecules. Previous studies have demonstrated that microorganisms are essential in NA degradation, exhibiting high genetic and metabolic diversity. This study investigated the biodegradation and regulatory properties of NA by the <em>Alcaligenes faecalis</em> JQ135 and the diversity and interrelationships of microbial metabolism of NA. NicR functions as a repressor in the <em>nic</em> cluster. An electrophoretic mobility shift assay (EMSA) demonstrated that NicR can bind to the promoter regions of R, C, B, A, and P operons in strain JQ135, with these multiple binding sites exhibiting the consensus sequence 5′-GTNNAC-3'. Bioinformatics analysis revealed 24 distinct <em>nic</em> cluster order types. The <em>nic</em> gene cluster in strain JQ135 comprises 15 genes arranged in two distantly located fragments and transcribed as five operons. These configurations are conserved within the genus <em>Alcaligenes</em> and are unique compared to the other 23 order types. The functional correlation among NicRs with differing degrees of similarity was examined, revealing that amino acid residues Q64 and V103 are crucial for NicR regulation. This study offers new molecular insights into the various regulatory mechanisms of bacterial degradation of NA in nature.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106103"},"PeriodicalIF":4.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling fungal degradation pathways for polyurethane and polyethylene through enrichment cultures and metabolic analysis","authors":"Eyalira Jacob Okal ,&nbsp;Jie Zhou ,&nbsp;Yanfei Wu ,&nbsp;Tingfang Zhong ,&nbsp;Yue Tang ,&nbsp;Zhengyu Sun ,&nbsp;Ruifang Xu ,&nbsp;Yuwei Hu ,&nbsp;Na Hu ,&nbsp;Jingxian Li ,&nbsp;Samantha C. Karunarathna ,&nbsp;Peter E. Mortimer ,&nbsp;Shahid Iqbal ,&nbsp;Dongmei Yu ,&nbsp;Jianchu Xu ,&nbsp;Heng Gui","doi":"10.1016/j.ibiod.2025.106097","DOIUrl":"10.1016/j.ibiod.2025.106097","url":null,"abstract":"<div><div>The increasing accumulation of plastics in the environment poses significant threats to marine and terrestrial ecosystems. However, recent research highlights the potential of <em>Lasiodiplodia iranensis</em>, a tropical ascomycete fungus, to degrade synthetic plastics. The intrinsic molecular mechanisms and metabolic responses during the interaction and hydrolysis of plastics, particularly for polyurethane (PU) and polyethylene (PE), remain largely unexplored. This study was conducted to investigate its degradation activity and metabolic responses to PU and PE and showed <em>L. iranensis</em> could colonise and significantly degrade PU (11.05 % weight loss), showcasing its impressive capabilities but having minimal effect on commercial PE (0.53 %) in 60 days. Metabolomic analysis identified 51 and 63 differentially expressed metabolites in response to PU and PE, respectively, with 30 common metabolites. Pathways for enzyme production, metal ion chelation, nutrient uptake, and Krebs cycle intermediates were activated in the fungus exposed to PU, likely contributing to its enhanced hydrolysis of PU. In contrast, pathways for stress response, antioxidant activity, signal transduction, and membrane integrity were predominant for PE, likely due to its limited degradability. Increased metabolism of compounds like 2-oxoarginine, proline, L-valine and 1-methyl histidine, which serve as carbon and nitrogen sources, osmoprotectants, and derivatives for fungal enzymes were observed in both treatments, thus supporting nutrient and enzyme synthesis. Hydrolytic and oxidative enzymes, mainly esterase, lipase, cutinase, laccase, and peroxidase, were implicated in PU and PE biodegradation, with PU showing more robust degradation potential. This study provides useful insights into the metabolic pathways that facilitate plastic degradation in <em>L. iranensis</em>, identifying potential fungal metabolites and enzymes that could be harnessed for bioremediation efforts, thereby advancing the development of fungal-based solutions for plastic waste reduction.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106097"},"PeriodicalIF":4.1,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the genomic and metabolic mechanisms of pyrene and phenanthrene degradation by Mycolicibacterium sp. SCSIO 43805: A high-Efficiency bacterium isolated from seagrass sediment","authors":"Manzoor Ahmad ,&nbsp;Tongyin Liang ,&nbsp;Yuhang Zhang ,&nbsp;Youshao Wang ,&nbsp;Jidong Gu ,&nbsp;Hao Cheng ,&nbsp;Khaled Masmoudi ,&nbsp;Weiguo Zhou ,&nbsp;Qingsong Yang ,&nbsp;Xiaofang Huang ,&nbsp;Junde Dong ,&nbsp;Juan Ling","doi":"10.1016/j.ibiod.2025.106101","DOIUrl":"10.1016/j.ibiod.2025.106101","url":null,"abstract":"<div><div>The ability of microorganisms to break down persistent organic pollutants in the environment has sparked significant interest in bioremediation, as it provides a firm foundation for implementing an effective, robust, and eco-friendly approach. In this study, we isolated a bacterium, identified as <em>Mycolicibacterium</em> sp. SCSIO 43805, from seagrass sediments and studied its potential for degrading pyrene and phenanthrene. The stain effectively degraded pyrene and phenanthrene, both provided at a concentration of 50 mg/L, within 12 days of incubation, as evidenced by the absence of any noticeable peaks related to pyrene and phenanthrene in the GC-MS profile of the 12-day extract. Moreover, the whole genome sequence analysis unveiled a comprehensive array of genes crucial for the complete degradation of polycyclic aromatic hydrocarbons. Multiple copies of dioxygenases such as <em>nidA</em>, <em>nidB</em> and <em>nidD</em> and monooxygenase such as cytochrome P450, which are essential for initial cleavage of aromatic rings, were detected in the genome of the <em>Mycolicibacterium</em> sp. SCSIO 43805. Furthermore, based on the gene contents and metabolic profiling of the extracts, we speculated that <em>Mycolicibacterium</em> sp. SCSIO 43805 could degrade pyrene, phenanthrene and other PAHs via phthalate and β-Ketoadipate pathway. The comparative genomics with other member of the genus <em>Mycolicibacterium</em> showed that <em>Mycolicibacterium</em> sp. SCSIO 43805 possessed a maximum number of genes involved in polycyclic aromatic hydrocarbon degradation. Hence, based on results of genomic, comparative genomics and metabolic profiling, <em>Mycolicibacterium</em> sp. SCSIO 43805 demonstrated high ability in degrading persistent organic pollutants and is an excellent biological agent for bioremediation of contaminated environment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106101"},"PeriodicalIF":4.1,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the impact of agrifood byproduct-based bioplastics on soil microbial communities and functioning","authors":"Marija Prodana ,&nbsp;Catarina Malheiro ,&nbsp;Joana Lopes ,&nbsp;Sara Peixoto ,&nbsp;Rui G. Morgado ,&nbsp;Idalina Gonçalves ,&nbsp;Paula Ferreira ,&nbsp;Manuel A. Coimbra ,&nbsp;Artur Alves ,&nbsp;Micael F.M. Gonçalves ,&nbsp;Sandra Hilário ,&nbsp;Susana Loureiro","doi":"10.1016/j.ibiod.2025.106083","DOIUrl":"10.1016/j.ibiod.2025.106083","url":null,"abstract":"<div><div>The environmental burden of plastic pollution has driven the search for sustainable alternatives, such as biodegradable bioplastics derived from agrifood byproducts, which hold the potential for addressing global waste management challenges. Given the close relationship between the biodegradability of biopolymers and soil microbial activities, it is vital to understand how the presence of novel bioplastics affects their function and structure. This study assessed microbial responses to different plastics: potato starch-based bioplastic, locust bean-based bioplastic, and non-biodegradable polyamide-polyethylene plastic in natural sandy-loam soil (LUFA 2.2). Soil without any (bio)plastics was used as a control. Despite initial pronounced differences in defragmentation rates between the two types of bioplastics, only 10 and 20 % w/w of the fragments were recovered after 17 weeks. The type of bioplastic influenced the fungal colonization pattern, with potato starch-based bioplastic resulting in more isolated fungal species than locust bean-based bioplastic. The soil dehydrogenase response was inconsistent, while β-glucosidase activity showed an initial increase in both bioplastic treatments, with a sustained stimulation only in potato starch-based bioplastic. β-glucosidase activity coincided with higher carbon substrate consumption maintained by the end of the 17 weeks of exposure, indicating more dynamic changes in microbial functions in potato starch-based bioplastic. Decreased carbon substrate consumption was observed in non-biodegradable polyamide-polyethylene plastic. The current study represents a first screening approach for the impact of applying agrifood byproducts as novel biopolymers directly in the soil, demonstrating their distinct effects without any pronounced adverse effects on soil microbiota compared to conventional polyamide-polyethylene plastic.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106083"},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reaction zone evolution and remediation efficacy evaluation of in-situ biogeochemical transformation of emulsified vegetable oil-FeSO4 enhanced tetrachloroethylene-contaminated aquifers","authors":"Chen Sun ,&nbsp;Jun Dong","doi":"10.1016/j.ibiod.2025.106100","DOIUrl":"10.1016/j.ibiod.2025.106100","url":null,"abstract":"<div><div>Enhanced in-situ biogeochemical transformation (ISBGT) has been proven effective in promoting the abiotic β-elimination of chlorinated solvents. However, the mechanisms underlying reaction zone evolution, remediation efficiency, and long-term permeability changes during the remediation process remain poorly understood. This study employed emulsified vegetable oil (EVO) and FeSO₄ as amendments to establish an in-situ reaction zone in a simulated column system. The reaction zone evolution was systematically analyzed, and the remediation efficiency and permeability variations in a PCE-contaminated aquifer were assessed. The results showed that after a single injection of EVO-FeSO₄, the reaction zone evolved through three distinct stages, including emulsified oil decomposition, microbial reduction, and β-elimination. The formation and aging mechanisms of the sulfur-iron mineral biogeobattery were also clarified. During the 300-day experimental period, the system achieved a PCE removal efficiency of 93.3%, with abiotic degradation processes contributing 96.36% of the total removal. This study provides important insights for the further development and practical application of ISBGT technology.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106100"},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation pathways of phthalate esters by Mycolicibacterium parafortuitum J101 and its ability to enhance bioremediation","authors":"Chatsuda Sakdapetsiri ,&nbsp;Chavisa Jeerasantikul ,&nbsp;Ritu Ningthoujam ,&nbsp;Adisan Rungsihiranrut ,&nbsp;Onruthai Pinyakong","doi":"10.1016/j.ibiod.2025.106085","DOIUrl":"10.1016/j.ibiod.2025.106085","url":null,"abstract":"<div><div>Phthalate esters (PAEs) are recalcitrant pollutants commonly used as plasticizers, and their degradation most effectively occurs by microorganisms. However, specific aspects of the degradation mechanism, particularly enzyme specificity and catalytic processes in the upper degradation pathway, remain unclear. Additionally, genes encoding esterases responsible for initial PAE hydrolysis have not been conclusively identified in many bacterial genera. In this study, <em>Mycolicibacterium parafortuitum</em> strain J101 was found to be capable of efficiently degrading and mineralizing three PAEs at an initial concentration of 100 mg/L in 7 days: short-chain dimethyl phthalate (88.57 %), diethyl phthalate (96.83 %), and dibutyl phthalate (99.37 %). Environmental conditions such as a pH less than 6 and greater than 5 % salinity affect the PAE degradation efficiency of strain J101. Genome mining revealed genes potentially involved in the complete degradation of PAEs by J101, which corresponds with J101's observed metabolic activity and gene expression. The <em>cut001</em>9 gene encodes a cutinase enzyme that is capable of hydrolyzing PAEs for initial degradation in the upper pathway. PAEs are transformed to phthalic acid, subsequently converted to protocatechuate and ultimately transformed to CO₂ and H₂O. A microcosm study revealed that the introduction of strain J101 significantly enhanced the efficiency of the degradation of mixed PAEs, and the strain synergized with indigenous microorganisms. Furthermore, the addition of strain J101 in landfill soil increased the diversity and complexity of interactions within the landfill soil bacterial community. Consequently, strain J101 demonstrates significant potential for application in the bioremediation of PAE-polluted environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106085"},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of crustacean shell waste by Halococcus salsus GUAg20; a chitinolytic and proteolytic halophilic archaeon","authors":"Gandisha Masso Pawar ,&nbsp;Sainil Vinod Naik ,&nbsp;Tejas Jagannath Naik ,&nbsp;Mrunal Karande ,&nbsp;Avinash Sharma ,&nbsp;Bhakti Balkrishna Salgaonkar","doi":"10.1016/j.ibiod.2025.106102","DOIUrl":"10.1016/j.ibiod.2025.106102","url":null,"abstract":"<div><div>Seafood plays a crucial role in meeting the global food, nutrition and employment demands; however, the rapid growth of fisheries and aquaculture has posed significant environmental challenges, especially in managing seafood waste. The traditional use of chemicals for seafood waste degradation causes pollution and ecological imbalance. Therefore, our study addresses this by exploring a halophilic archaeon, <em>Halococcus salsus,</em> isolated from salt crystals of the Goan saltpan and identified through 16S rRNA sequencing. The halophilic archaeal isolate GUAg20, was applied in the bioprocessing of shrimp shell waste (SSW) and resulted in its degradation with a high demineralization efficiency of 93.08 % and a weight loss of 65.7 %. In the fermentation medium, the chitinolytic activity of the isolate GUAg20 was confirmed by observing an exponential release of N-acetylglucosamine (NAG) corresponding to 1.64 <span><math><mrow><mo>×</mo></mrow></math></span> 10³, 2.18 <span><math><mrow><mo>×</mo></mrow></math></span> 10³, 2.88 <span><math><mrow><mo>×</mo></mrow></math></span> 10³ and 4.35 <span><math><mrow><mo>×</mo></mrow></math></span> 10³ mg/L after 5, 10, 15 and 20 days, respectively. The degradation of SSW was further confirmed using field emission scanning electron microscopy (FE-SEM) and fourier transform infrared spectroscopy (FTIR). This study reports the first use of <em>Halococcus salsus</em> GUAg20, a chitinolytic, proteolytic and gelatinolytic halophilic archaeon, for marine crustacean waste biodegradation, while concurrently focusing on seafood waste management.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106102"},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced tolerance of Saccharomyces cerevisiae to industrial inhibitors through multi-transcription factor engineering for environmental and biotechnological applications","authors":"Zhengyue Zhang ,&nbsp;Difan Xiao ,&nbsp;Hanyu Wang ,&nbsp;Qian Li ,&nbsp;Sardar Ali ,&nbsp;Yulei Chen ,&nbsp;Jiaye Tang ,&nbsp;Linjia Jiang ,&nbsp;Jiwei Shen ,&nbsp;Wenli Xin ,&nbsp;Lingling Feng ,&nbsp;Menggen Ma","doi":"10.1016/j.ibiod.2025.106099","DOIUrl":"10.1016/j.ibiod.2025.106099","url":null,"abstract":"<div><div>This study presents a novel approach to enhance the tolerance of Saccharomyces cerevisiae to industrial inhibitors, including furfural, 5-hydroxymethylfurfural (HMF), acetic acid, formic acid, anhydrous ethanol, and phenol. By co-overexpressing the transcription factors PDR1, YAP1, and RPN4, engineered yeast strains exhibited significantly improved stress resistance, with shorter lag phases and higher growth rates compared to parental strains. RNA sequencing revealed upregulation of key genes involved in NADPH regeneration, redox balance, and cell membrane stabilization, while downregulation of protein modification genes suggested an energy-efficient oxidative stress management strategy. These findings demonstrate the potential of multi-transcription factor engineering to improve yeast strain performance under toxic conditions, with important implications for industrial bioethanol production and environmental pollutant degradation. Future research should focus on optimizing these strains for broader industrial applications, including scaling up fermentation processes to enhance bioethanol yield and environmental remediation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106099"},"PeriodicalIF":4.1,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efflux pumps of Pseudomonas aeruginosa and their regulatory mechanisms underlying multidrug resistance","authors":"Wen-Ru Li ,&nbsp;Zhi-Qing Zhang ,&nbsp;Kang Liao ,&nbsp;Qing-Shan Shi ,&nbsp;Xu-Bin Huang ,&nbsp;Xiao-Bao Xie","doi":"10.1016/j.ibiod.2025.106096","DOIUrl":"10.1016/j.ibiod.2025.106096","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em>, an opportunistic gram-negative bacterial pathogen, is a significant threat in hospital intensive care units due to its ability to cause various human infections. Its large genome enables remarkable adaptability to environmental changes, resulting in the development of antibiotic and multidrug resistance (MDR). The chromosomes of <em>P. aeruginosa</em> strains harbor numerous resistance genes, the majority of which are related to efflux pumps (EPs). These genes are primarily responsible for antibiotic and MDR. Efflux systems are pivotal in <em>P. aeruginosa</em> MDR, comprising six major EP protein families: ATP-binding cassette, major facilitator, multidrug and toxin extrusion, small MDR, proteobacterial antimicrobial compound efflux, and resistance nodulation cell division (RND) superfamilies. Among these, RND EPs are the most critical, displaying the broadest substrate spectrum and the strongest correlation with MDR. The <em>P. aeruginosa</em> genome encodes twelve RND EPs, which exhibit overlapping but distinct substrate ranges. Notably, MexAB, MexXY, MexCD, and MexEF EPs contribute significantly to MDR. EP systems in <em>P. aeruginosa</em> are unique, with gene sequences distinct from those of EP systems in other gram-negative and gram-positive bacteria, making interspecific gene transfer of EP resistance genes uncommon. EP inhibitors (EPIs) possess the potential to promote the clinical efficacy of antibiotics against <em>P. aeruginosa</em> infections. However, no EPIs have been applied in clinical anti-infective treatment to date. Consequently, there is an urgent need for in-depth exploration of the molecular structures, functions, and mechanisms of <em>P. aeruginosa</em> EP systems. Developing low-toxicity, high-efficacy, and broad-spectrum EPIs is crucial to drive and accelerate their clinical application.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106096"},"PeriodicalIF":4.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}