International Biodeterioration & Biodegradation最新文献

{"title":"Linking microbial community dynamics to electrochemical corrosion behaviour of copper-nickel and titanium in simulated marine flow conditions","authors":"Kiran Kumar Vadde,&nbsp;Luis Perdomo-Hurtado,&nbsp;Jorge Escribano,&nbsp;Alejandro Morales Betancourt,&nbsp;Oladis Troconis de Rincón,&nbsp;Muhammad Awais Javed,&nbsp;Scott Wade,&nbsp;Jason S. Lee,&nbsp;Brendy Rincón Troconis,&nbsp;Vikram Kapoor","doi":"10.1016/j.ibiod.2026.106459","DOIUrl":"10.1016/j.ibiod.2026.106459","url":null,"abstract":"<div><div>This study investigated microfouling community succession and diversity and their effect on corrosion of copper-nickel 90/10 (CuNi) and Grade 2 titanium (Ti) under simulated marine low-flow conditions over 16 weeks. Total and active microbial communities were characterized using 16S/18S rRNA gene sequencing from genomic and complementary DNA, while qPCR quantified functional genes for copper resistance and biofilm formation. Sequencing yielded 1980 cDNA and 6596 gDNA amplicon sequence variants, spanning 32 phyla on CuNi and 30 phyla on Ti, with Proteobacteria and Bacteroidetes comprising ∼85% of the CuNi community. CuNi biofilms hosted <em>Marinobacter</em> and Cytophagales members as early colonizers, while Ti supported a more diverse early community including predatory Bdellovibrionaceae. Sulfate-reducing, sulfur-oxidizing, and acid-producing bacteria on CuNi indicated elevated MIC; sulfur-reducing bacteria with MIC potential were also detected on Ti. Raman spectroscopy revealed less protective CuO/Cu(OH)₂ on CuNi, while Ti maintained TiO₂ film with transient Ti₂O₃. Localized pitting occurred on CuNi (maximum depth 5.27 μm; maximum pit area 677.91 μm²) but not Ti. Electrochemical characterization demonstrated spatial heterogeneity in corrosion resistance linked to flow velocity: CuNi and Ti coupons at the inlet (&gt;1.5 m/s) showed higher polarization and charge-transfer resistance (e.g., CuNi inlet Rct increased ∼10-fold from week 2 to 16), while outlet coupons (&lt;0.6 m/s) exhibited declining corrosion resistance over time (CuNi outlet Rp peaked at 35,094 Ω cm² at week 4 before declining by week 16). Ti inlet coupons demonstrated repassivation by week 16. Overall, this study offers novel insights into the MIC of examined metals under low-flow conditions by integrating DNA/RNA-based biofilm analyses with comprehensive surface and electrochemical characterization.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"215 ","pages":"Article 106459"},"PeriodicalIF":4.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148959287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Bacillus clausii-based biosensor for heavy metal toxicity: Effects of organic substrates on sensitivity and application to electroplating wastewater","authors":"Hoang-Duy P. Nguyen,&nbsp;Phuong-Thao T. Le,&nbsp;Duc-Thang Tran,&nbsp;Anh Minh-Nhat Lai,&nbsp;Thanh-Linh H. Duong,&nbsp;Tien-Cuong Hoang,&nbsp;Tang Ba Quang,&nbsp;Nguyen Phuc Thanh Duy,&nbsp;Ta Dang Khoa,&nbsp;Thuy-Phuong T. Pham","doi":"10.1016/j.ibiod.2026.106461","DOIUrl":"10.1016/j.ibiod.2026.106461","url":null,"abstract":"&lt;div&gt;&lt;ul&gt;&lt;li&gt;&lt;span&gt;•&lt;/span&gt;&lt;span&gt;&lt;div&gt;A respiration-based &lt;em&gt;B. clausii&lt;/em&gt; biosensor was developed for metal toxicity.&lt;/div&gt;&lt;/span&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;•&lt;/span&gt;&lt;span&gt;&lt;div&gt;50% BHI enhanced Cr(VI) sensitivity while minimizing organic interference.&lt;/div&gt;&lt;/span&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;•&lt;/span&gt;&lt;span&gt;&lt;div&gt;Zn(II) showed the strongest inhibition among tested metals.&lt;/div&gt;&lt;/span&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;•&lt;/span&gt;&lt;span&gt;&lt;div&gt;Toxicity detected in electroplating effluent below discharge limits.&lt;/div&gt;&lt;/span&gt;&lt;/li&gt;&lt;/ul&gt;&lt;/div&gt;&lt;div&gt;&lt;div&gt;Electroplating wastewater (EW) contains complex mixtures of heavy metals that pose significant ecological risks while conventional chemical analyses provide limited insight into their combined biological toxicity. In this study, a respiration-based biosensor using &lt;em&gt;Bacillus clausii&lt;/em&gt; was developed for detection of heavy metal toxicity in aqueous systems. Sensitivity optimization showed that using brain heart infusion (BHI) medium at 50% strength as both the growth medium and respiration substrate significantly enhanced the sensitivity of &lt;em&gt;B. clausii&lt;/em&gt; to Cr(VI) while preventing interference from organic matter representative of wastewater matrices in CO&lt;sub&gt;2&lt;/sub&gt;-based respiration measurements. In synthetic wastewater, the biosensor exhibited reproducible and concentration-dependent responses to Cr(VI), Ni(II), and Zn(II) with their inhibitory effect following the order Zn(II) &gt; Ni(II) &gt; Cr(VI). Although increasing organic load partially reduced metal toxicity, the relatively low biochemical oxygen demand (BOD) of EW maintained favorable conditions for high biosensor sensitivity. Application to real EW samples collected from multiple sites confirmed the practical applicability of the biosensor, showing excellent repeatability and reproducibility across independent cultures. Statistically significant toxicity was detected at total metal concentrations as low as 0.168 mg L&lt;sup&gt;−1&lt;/sup&gt; which is below the lowest permissible discharge limits according to QCVN 40:2025/BTNMT. Interestingly, treated EW which meet discharge standard specified in Column B of QCVN 40:2025/BTNMT still exhibited toxicity, suggesting the limitations of common assessments that rely only on physico-chemical indicators. Additionally, the &lt;em&gt;B. clausii&lt;/em&gt;-based biosensor demonstrated higher sensitivity than Microtox while exhibiting a faster detection time and simpler operation than &lt;em&gt;Daphnia&lt;/em&gt; tests, supporting its potential for early-warning purposes.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;Electroplating wastewater (EW) contains complex mixtures of heavy metals that pose significant ecological risks while conventional chemical analyses provide limited insight into their combined biological toxicity. In this study, a respiration-based biosensor using &lt;em&gt;Bacillus clausii&lt;/em&gt; was developed for detection of heavy metal toxicity in aqueous systems. Sensitivity optimization showed that using brain heart infusion (BHI) medium at 50% strength as both the growth medium and respiration su","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"215 ","pages":"Article 106461"},"PeriodicalIF":4.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148959284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flagellum proteins found role in biofilm and larval metamorphosis","authors":"Ji-Dong Gu","doi":"10.1016/j.ibiod.2026.106420","DOIUrl":"10.1016/j.ibiod.2026.106420","url":null,"abstract":"","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"215 ","pages":"Article 106420"},"PeriodicalIF":4.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148959286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Better evidence required on biodegradability of PFAS","authors":"Ji-Dong Gu","doi":"10.1016/j.ibiod.2026.106458","DOIUrl":"10.1016/j.ibiod.2026.106458","url":null,"abstract":"<div><div>Convincing scientific results are still seriously missing on the biodegradability of per- and poly-fluoroalkyl substances (PFAS). Further research advancement on this topic shall be focused on the isolation of the capable/responsible microorganism, the biochemical transformation reaction and pathway, and stoichiometry logically to establish a good ground and basis for the biodegradation of them. With these, further in-depth physiology and biochemistry of the organism and more importantly the enzymes can be elucidated in a fundamental manner. Best methods and an integrated approaches shall also be considered to best serve the delineation and substantiation of the science on this selected topic for a better and comprehensive knowledge.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"215 ","pages":"Article 106458"},"PeriodicalIF":4.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148959285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of subaerial algae on building materials by a new standardised imaging Pulse-Amplitude-Modulation Fluorometry (PAM-F) method","authors":"Leonie Stohl,&nbsp;Jake Cook,&nbsp;Julia von Werder,&nbsp;Anna A. Gorbushina,&nbsp;Chiara Tonon","doi":"10.1016/j.ibiod.2026.106455","DOIUrl":"10.1016/j.ibiod.2026.106455","url":null,"abstract":"<div><div>Imaging Pulse-Amplitude-Modulation Fluorometry (PAM-F) is a quantitative, sensitive, and non-destructive tool for detecting early biofilm establishment, yet its broader use has been limited by the lack of standardised protocols and by uncertainties arising from material-specific effects. In this study, the first comprehensive protocol is presented considering substrate properties and achieving reproducible dark fluorescence yield (F₀) measurements even for low algal cell densities. Our workflow combines standardised sample preparation with an algorithm-driven evaluation to generate calibration curves, detection limits, and optimal device settings. By applying two defined inoculation ranges of the subaerial green alga <em>Jaagichlorella</em> sp. AB13.021D5 to marble and concrete pellets, and polycarbonate membranes, we show that F₀ intensity reflects not only algal abundance and device settings but also physical and mineralogical substrate properties. Particularly the high reflectance of marble increased noise and oversaturation effects, demonstrating the necessity of substrate-specific calibration. This transferable protocol establishes <em>Jaagichlorella</em> sp. AB13.021D5 as a test organism for comparative and application-oriented bioreceptivity studies (such as evaluating material susceptibility, comparing protective treatments, and monitoring early colonisation). By delivering validated measurement settings as well as substrate-specific calibration curves and detection limits for Imaging PAM-F studies, this work closes a critical methodological gap and supports the development of evidence-based biodeterioration management strategies.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"215 ","pages":"Article 106455"},"PeriodicalIF":4.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148959280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indole biodegradation by Burkholderia contaminans DM32: Process characteristics and the IifR-mediated molecular mechanism","authors":"Ying-Si Wang,&nbsp;Jie Wang,&nbsp;Hong-Bing Tao,&nbsp;Hui-Zhong Liu,&nbsp;Bei-Bei Wang,&nbsp;Zhen-Lin Liao,&nbsp;Hong Qi,&nbsp;Qing-Shan Shi,&nbsp;Gang Zhou,&nbsp;Xiao-Bao Xie","doi":"10.1016/j.ibiod.2026.106460","DOIUrl":"10.1016/j.ibiod.2026.106460","url":null,"abstract":"<div><div>Indole is a recalcitrant <em>N</em>-heterocyclic pollutant released from livestock and industrial waste, posing serious ecological and toxicological hazards. Despite numerous reported indole-degrading bacteria, the molecular regulatory circuits of indole catabolism within <em>Burkholderia</em> remain poorly resolved. In this study, a novel indole-degrading bacterium, <em>Burkholderia contaminans</em> DM32, was isolated from contaminated production water. Strain DM32 exhibited robust indole-removal capacity across broad environmental ranges with optimal degradation performance at 30 °C and pH 5.0-7.0, accompanied by indigo formation as a metabolic by-product. Notably, DM32 achieved complete indole removal from livestock manure wastewater within 24 h, demonstrating its feasibility for pollutant remediation in complex real-world matrices. Genomic and functional assays revealed that indole-induced flavoproteins IifCD are indispensable for both indole degradation and indigo biosynthesis. Transcriptomic profiling implies the existence of auxiliary oxidative pathways that may functionally compensate for loss of the canonical <em>iif</em>-driven catabolism. Furthermore, we identified the AraC/XylS-family transcriptional regulator IifR as a key positive regulator of the <em>iif</em> gene cluster. Mechanistically, indole acts as an effector molecule to trigger <em>iifR</em> expression, and the resulting IifR protein specifically recognizes and binds the palindromic CGGATA motif (−236 to −258) within the <em>iif</em> promoter via its C-terminal domain, thereby activating transcription of the downstream <em>iif</em> gene cluster, while the N-terminal domain is indispensable for transcriptional activation rather than DNA recognition. Collectively, this study reveals the catalytic-regulatory framework of indole catabolism in <em>B</em>. <em>contaminans</em> DM32, offering a promising strain resource for indole-polluted wastewater bioremediation and bio-indigo production.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"215 ","pages":"Article 106460"},"PeriodicalIF":4.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148959283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of organophosphorus pesticide-degrading bacteria and a ratio-optimized consortium for efficient phoxim and malathion removal with pathway inference","authors":"Xinran Tang,&nbsp;Mazna Waheed,&nbsp;Qi Xu,&nbsp;Xingrong Liu,&nbsp;Rongrong Xie,&nbsp;Alei Geng,&nbsp;Daochen Zhu","doi":"10.1016/j.ibiod.2026.106419","DOIUrl":"10.1016/j.ibiod.2026.106419","url":null,"abstract":"<div><div>Organophosphorus (OP) pesticides remain as ecotoxic residues in soil and water bodies, and microbial consortia represent a viable bioremediation strategy. Eight OP-degrading bacterial candidates were identified from agricultural soil and irrigation water in Jiangsu, China. Strain PA1, closely related to <em>Pseudomonas aeruginosa</em> JCM 5962 (99.6% 16S rRNA gene similarity), attained 61.0% abiotic-corrected phoxim removal under the initial monoculture screening condition (100 mg L⁻¹, 72 h). Response surface methodology provided a validated operational range of 37°C, pH 7.4, and 4% inoculum, resulting in an 84.4% removal rate. A consortium of PA1 and <em>Stenotrophomonas pavanii</em> SP1 (3:1, 3% of total inoculum) achieved an 87.4% removal of phoxim in single-substrate assays and retained 87.6% phoxim and 85.8% malathion removal in mixed-substrate tests. Liquid chromatography-mass spectrometry detected phoxim-oxon and O, O-diethyl thiophosphate, indicating concurrent hydrolytic and oxidative pathways at the Enzyme Commission class level. Considering the close relationship between PA1 and <em>P. aeruginosa</em>, practical application should consider immobilized cells, cell-free systems, or transfer of catabolic modules to non-pathogenic hosts. This established consortium and its tested framework offer a replicable, evidence-conservative platform for OP reduction.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"214 ","pages":"Article 106419"},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148635430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Genomic and functional characterization of an Antarctic strain of Rhodosporidiobolus odoratus and its hydrocarbon-degradation potential” [Int. Biodeterior. Biodegrad. 214 (2026) 106379]","authors":"G. Cavallini,&nbsp;M.A. Guerreiro,&nbsp;E. Stukenbrock,&nbsp;F. Canini,&nbsp;P. Buzzini,&nbsp;S. Tosi,&nbsp;B. Turchetti,&nbsp;M. Groenewald,&nbsp;S. Garzoli,&nbsp;L. Zucconi","doi":"10.1016/j.ibiod.2026.106416","DOIUrl":"10.1016/j.ibiod.2026.106416","url":null,"abstract":"","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"214 ","pages":"Article 106416"},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148634575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Petroleum microbiology using tiny water droplets","authors":"Ji-Dong Gu","doi":"10.1016/j.ibiod.2026.106418","DOIUrl":"10.1016/j.ibiod.2026.106418","url":null,"abstract":"","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"214 ","pages":"Article 106418"},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148636154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New development on cultural heritage microbiology in China","authors":"Xiaobo Liu,&nbsp;Fasi Wu,&nbsp;Youzhi Feng,&nbsp;Ji-Dong Gu","doi":"10.1016/j.ibiod.2026.106374","DOIUrl":"10.1016/j.ibiod.2026.106374","url":null,"abstract":"<div><div>Culture heritage microbiology is an emerging inter-discipline of cultural heritage and microbiology, which is increasingly developing to meet the necessity of sustainable conservation of world cultural heritage, especially in China. Here, we report a scientific session featuring a special topic of Microorganisms and Cultural Heritage Conservation, which was convened and established for the first at the 14th Annual Geomicrobiology Conference of China in Lanzhou of China on April 17-19, 2026. During this session, 26 presentations on the latest research advances and findings of culture heritage microbiology were delivered by scholars from across the country, followed by active academic discussions and exchanges. The successful convening of this session attracted enthusiastic participation and activated multidiscipline thought exchanges, including microbiology, cultural heritage conservation, environmental science, materials science, engineering, and geology, which will largely promote the innovative development of advanced technologies in the interdisciplinary field of geomicrobiology and cultural heritage conservation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"214 ","pages":"Article 106374"},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148636153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}