International Biodeterioration & Biodegradation最新文献

Bioactivity and antibiofilm activity of metal and non-metal nanoparticles in DES against resistant microorganisms DES中金属和非金属纳米颗粒对耐药微生物的生物活性和抗生物膜活性

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-27 DOI: 10.1016/j.ibiod.2025.106121

Olga Długosz , Anna Żebracka , Wioletta Wnuk , Agata Drabik , Marta Sochocka , Dominika Franz , Anna Chmielowiec-Korzeniowska , Marcin Banach

{"title":"Bioactivity and antibiofilm activity of metal and non-metal nanoparticles in DES against resistant microorganisms","authors":"Olga Długosz , Anna Żebracka , Wioletta Wnuk , Agata Drabik , Marta Sochocka , Dominika Franz , Anna Chmielowiec-Korzeniowska , Marcin Banach","doi":"10.1016/j.ibiod.2025.106121","DOIUrl":"10.1016/j.ibiod.2025.106121","url":null,"abstract":"<div><div>The increasing resistance of microorganisms to antibiotics necessitates the search for new therapeutic strategies, including the use of nanoparticles with bioactive properties. The aim of this study was to investigate the antibiofilm and antimicrobial properties of metal and non-metal (Ag, Cu, Se) nanoparticles, obtained in deep eutectic solvent (DES), against resistant bacterial strains. The study investigated the determination of the minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of selected Me-DES systems. The results showed that the MBCs of <em>S. aureus</em> ATCC 700699 and <em>E. coli</em> ATCC BAA -2523 for Ag-DES were 18.2 and 15.6 μg/mL, respectively, while those for Cu-DES and Se-DES were 125 μg/mL. Serial passages of bacterial cells were performed to assess the ability of microorganisms to develop resistance to the test systems, indicating their limited adaptability against Ag-DES and Cu-DES (MIC maintenance for 24 passages). The antibiofilm activity of the nanoparticles was confirmed by their ability to remove 12–37 % of <em>S.Aureus</em> bacterial biofilm and 23–51 % of <em>E.coli</em> biofilm after 24 h of exposure. In addition, the MTT cytotoxicity test showed that Me-DES systems exhibited low cytotoxicity against cell lines (A549). Analysis of the mobility of microorganisms after exposure to nanoparticles indicated a reduction in mobility capacity in the range of 14–68 % already at a concentration of 31.3 μg/mL, depending on the nanoparticles used. The study demonstrates that metal and non-metal nanoparticles in DES represent a promising system to combat resistant microorganisms, while reducing their ability to develop resistance and destroying the bacterial biofilm.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106121"},"PeriodicalIF":4.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential oils in hydrogel for microalgal biofilm removal: Application strategies for stone heritage preservation 去除微藻生物膜的水凝胶精油：用于石材遗产保护的应用策略

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-24 DOI: 10.1016/j.ibiod.2025.106128

Roberta Ranaldi , Francesco Gabriele , Lorenza Rugnini , Patrick Di Martino , Rémy Agniel , Francesco Scuderi , Roberto Braglia , Antonella Canini , Nicoletta Spreti

{"title":"Essential oils in hydrogel for microalgal biofilm removal: Application strategies for stone heritage preservation","authors":"Roberta Ranaldi , Francesco Gabriele , Lorenza Rugnini , Patrick Di Martino , Rémy Agniel , Francesco Scuderi , Roberto Braglia , Antonella Canini , Nicoletta Spreti","doi":"10.1016/j.ibiod.2025.106128","DOIUrl":"10.1016/j.ibiod.2025.106128","url":null,"abstract":"<div><div>This study faces the critical need to develop new eco-friendly biocides and effective application strategies to mitigate phototrophic biodeteriogens in cultural heritage sites. To address this challenge, a microalgal strain previously collected from the hypogeum of the Colosseum (Rome, Italy) was used to induce biofilm formation on Lecce stone specimens. The samples were treated with 0.5% and 1% of essential oils (EOs) from <em>Thymus vulgaris</em> L., <em>Origanum vulgare</em> L. and <em>Cinnamomum verum</em> Presl. The EOs were encapsulated in an alginate hydrogel support matrix (HG) and applied to the biofilms with different application times (24 h and 48 h). A mini-PAM portable fluorometer was used to determine the phototrophic activity up to 2 months after treatment. Scanning electron microscopy was used to investigate the effects of EOs on microalgal cell morphology within biofilms, while Fourier transform infrared spectroscopy analyzed changes in biomolecular distribution after treatment. The findings revealed that all EOs were effective when encapsulated in HG. However, by the end of the monitoring period, only cinnamon EO maintained photosynthetic inhibition, especially when applied at 0.5% for 48 h. Moreover, biofilm treated with cinnamon EO showed the most significant effects, particularly in disrupting cell membranes and reducing lipids signals, ultimately leading to cell lysis. This approach effectively inhibited the vitality of biofilm-forming phototrophs on stone surfaces, using low concentrations of EO for defined periods of time.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106128"},"PeriodicalIF":4.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative strategy for dechlorination of halogenated organic Pollutants: Microbial-Derived formate production and Immobilized-PdNP biosynthesis 卤化有机污染物脱氯的创新策略：微生物衍生甲酸生产和固定化pdnp生物合成

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-23 DOI: 10.1016/j.ibiod.2025.106129

Yang-Yang Fan, Wen-Wen Liu, Yu-Han Chai, Jing Huang, Min Wang, Xin Wang, Xin-Lu Cai, Li Zhang, Xiang Xiao

{"title":"Innovative strategy for dechlorination of halogenated organic Pollutants: Microbial-Derived formate production and Immobilized-PdNP biosynthesis","authors":"Yang-Yang Fan, Wen-Wen Liu, Yu-Han Chai, Jing Huang, Min Wang, Xin Wang, Xin-Lu Cai, Li Zhang, Xiang Xiao","doi":"10.1016/j.ibiod.2025.106129","DOIUrl":"10.1016/j.ibiod.2025.106129","url":null,"abstract":"<div><div>Halogenated organic pollutants (HOPs) are accumulating in the environment, posing significant ecological risks. Microbial-driven palladium nanoparticles (PdNPs) facilitate efficient reductive hydrodehalogenation, offering a promising approach for HOPs remediation. To address the limitations associated with hydrogen donor addition and catalyst detachment, this study proposed a proof-of-concept strategy for developing a novel two-stage dehalogenation reactor. We initially constructed genetically engineered <em>Shewanella oneidensis</em> MR-1 to enhance the production and accumulation of formate as the hydrogen donor. Subsequently, encapsulated PdNPs was biosynthesized within alginate beads for efficient immobilization. Finally, a two-stage reactor was constructed for dechlorination of 2,4,6-trichlorophenol (2,4,6-TCP). In the first-stage reactor, the immobilized engineered strain accumulated 2.04 mM formate, providing sufficient hydrogen donors. In the second-stage reactor, 40 μM 2,4,6-TCP was completely dechlorinated within 10 h. Reactor performance was optimized through pH, lactate concentration, and initial pollutant load. Degradation product analysis revealed that dechlorination of 2,4,6-TCP was occurred via a one-electron transfer reaction, with the chlorinated intermediates being progressively hydrodechlorinated until the final product phenol. Furthermore, this constructed reactor exhibited high dechlorination efficiency for 2,4,6-TCP in real water samples and showed remediation potential for other HOPs. This study provides a promising strategy for the effective management of halogenated wastewater pollution.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106129"},"PeriodicalIF":4.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of polyethylene glycol and various carboxylic acids on the biological durability of beech wood (Fagus sylvatica) and Scots pine sapwood (Pinus sylvestris) 聚乙二醇和各种羧酸对山毛榉木和苏格兰松材生物耐久性的影响

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-23 DOI: 10.1016/j.ibiod.2025.106127

Melissa Christ , Nicole Flaig , Christian Brischke , Holger Militz , Marcus Müller

{"title":"Influence of polyethylene glycol and various carboxylic acids on the biological durability of beech wood (Fagus sylvatica) and Scots pine sapwood (Pinus sylvestris)","authors":"Melissa Christ , Nicole Flaig , Christian Brischke , Holger Militz , Marcus Müller","doi":"10.1016/j.ibiod.2025.106127","DOIUrl":"10.1016/j.ibiod.2025.106127","url":null,"abstract":"<div><div>The goal of this study was to investigate the impact of wood modification with polyethylene glycol (PEG) 400 and various carboxylic acids (citric acid (CA), malic acid (MA) and 1,2,3,4-butanetetracarboxylic acid (BTCA)) on the biological durability of beech wood (<em>Fagus sylvatica</em>) and Scots pine sapwood (<em>Pinus sylvestris</em>). For the brown, white and soft rot experiments, mass losses (ML) were calculated according to the respective standards. Weight percent gain, bulking and anti-swelling efficiency were determined to determine the quality of the modifications. Modifying agents, fixed inside the wood, demonstrated anti-swelling efficiency after leaching cycles (beech wood: 9–46 %). The decay resistance against brown and white rot fungi was considerably improved, with PEG/CA and PEG/BTCA being more effective than PEG/MA. Beech wood modified with PEG/CA achieved durability class (DC) 1–2, PEG/BTCA reached DC 1 and PEG/MA fell into DC 2–4. For Scots pine sapwood, PEG/CA and PEG/BTCA reached DC 1, while PEG/MA only achieved DC 4. The biological durability against soft rot fungi was less affected, and curing alone did not enhance the resistance to soft rot fungi. PEG/BTCA achieved DC 1, while PEG/MA and PEG/CA ranged between DC 1 and DC 3. The variants with different CA to PEG ratios as well as CA without PEG showed no major differences in terms of ML due to soft rot fungi. In conclusion, the modifications resulted in significantly lower mass losses.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106127"},"PeriodicalIF":4.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon steel Q235 corrosion protection by metabolism of Bacillus cereus biofilms in cooling water environment 蜡样芽孢杆菌生物膜在冷却水环境中的代谢对碳钢Q235的防腐作用

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-22 DOI: 10.1016/j.ibiod.2025.106124

Yanglin Hu , Benxiang Guo , Yifei Wang , Fangwei Li , Xudong He , Qingyun Niu , Xiangyu Cui , Qingquan Wei , Xiaobang Hou , Lefeng Li

{"title":"Carbon steel Q235 corrosion protection by metabolism of Bacillus cereus biofilms in cooling water environment","authors":"Yanglin Hu , Benxiang Guo , Yifei Wang , Fangwei Li , Xudong He , Qingyun Niu , Xiangyu Cui , Qingquan Wei , Xiaobang Hou , Lefeng Li","doi":"10.1016/j.ibiod.2025.106124","DOIUrl":"10.1016/j.ibiod.2025.106124","url":null,"abstract":"<div><div>Multiple types of microorganisms have been reported to provide a corrosion protective effect on metals, but few cases under cooling water environments. This study addresses this research gap by employing <em>Bacillus cereus</em> (<em>B. cereus</em>) biofilms for carbon steel (CS) Q235 corrosion protection. The results demonstrated that the addition of <em>B. cereus</em> agent reduced the weight loss of CS Q235 by 92.38 %, increased the electrochemical impedance (<em>R</em><sub><em>ct</em></sub> 21616.77 Ω/cm<sup>2</sup>) and lowered the corrosion current density (<em>I</em><sub><em>corr</em></sub> 16.8 μA cm<sup>−2</sup>). Surface analysis indicated that <em>B. cereus</em> cells secreted polysaccharides and proteins to form a biofilm. Such biofilm, combined with biomineralized corrosion products, formed a composite layer that prevented direct contact between CS Q235 and corrosive media. Moreover, metabolites of <em>B. cereus</em> biofilm associated with corrosion protective capacity were profiled and screen by untargeted metabolism analysis. A total of 376 metabolites were detected and genetic materials including uridine monophosphate (UMP) and guanosine monophosphate (GMP) were identified as the most critical metabolic principle for corrosion protection. These metabolites, which enriched in nucleotide metabolic pathways, was considered as metabolic mechanisms of corrosion protection. The <em>B. cereus</em> cells forming extracellular polymeric substance (EPS)-biofilm to alter the physicochemical properties of CS Q235 surface and repelled the attachment of corrosive microorganisms, thus hindered their multiplication and protected CS Q235 from corrosion in cooling water environment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106124"},"PeriodicalIF":4.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and transcriptomic insights into benzoate and 4-hydroxybenzoate degradation in Arthrobacter sp. PAMC25564 isolated from cryoconite 从低温孢子中分离出来的PAMC25564节杆菌对苯甲酸盐和4-羟基苯甲酸盐降解的基因组学和转录组学研究

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-19 DOI: 10.1016/j.ibiod.2025.106125

Nisha Ghimire , Byeollee Kim , So-Ra Han , Jun Hyuck Lee , Tae-Jin Oh

{"title":"Genomic and transcriptomic insights into benzoate and 4-hydroxybenzoate degradation in Arthrobacter sp. PAMC25564 isolated from cryoconite","authors":"Nisha Ghimire , Byeollee Kim , So-Ra Han , Jun Hyuck Lee , Tae-Jin Oh","doi":"10.1016/j.ibiod.2025.106125","DOIUrl":"10.1016/j.ibiod.2025.106125","url":null,"abstract":"<div><div><em>Arthrobacter</em> sp. has long been recognized for its metabolic versatility, particularly in the degradation of xenobiotic compounds. They have been isolated from diverse environments, including extreme environments. In this study, we conducted biotransformation, genomics and transcriptomics of <em>Arthrobacter</em> sp. PAMC25564 isolated from the cryoconite of Wurmkogel, Ötztaler Alps, Austria, one of the extreme environments to unveil its aromatic compound-degrading potential. The strain possesses several genes associated with aromatic compound catabolism including at least one complete pathway to utilize two peripheral intermediates, 4-hydroxybenzoate (4-HB) and benzoate, which are mostly determined as wastewater contaminants. <em>Arthrobacter</em> sp. PAMC25564 tolerated and degraded benzoate and 4-HB up to 25 mM. Further, RNA-seq transcriptomics was done to investigate the benzoate and 4-HB degradation pathway in PAMC25564. The results indicate global gene regulation and pathway redundancy. Furthermore, growth of the strain in these aromatic compounds upregulates multiple genes associated with amino acid metabolism and phenylacetate degradation, which are associated with stress response. These findings contribute to a better understanding of aromatic compound degradation with a special focus on wastewater contaminants, benzoate and 4-HB degradation in <em>Arthrobacter</em> sp. PAMC25564.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106125"},"PeriodicalIF":4.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrocatalytic oxidation of bisphenol A using optimized Fe/Cu cathodic catalysts: Biotoxicity and microbial community analysis in bioelectro-Fenton systems 优化的Fe/Cu阴极催化剂电催化氧化双酚A：生物毒性和生物电fenton系统的微生物群落分析

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-17 DOI: 10.1016/j.ibiod.2025.106126

Shu-Hui Liu , Chang-Ting Hsieh , Chi-Wen Lin , Yen San Chan

{"title":"Electrocatalytic oxidation of bisphenol A using optimized Fe/Cu cathodic catalysts: Biotoxicity and microbial community analysis in bioelectro-Fenton systems","authors":"Shu-Hui Liu , Chang-Ting Hsieh , Chi-Wen Lin , Yen San Chan","doi":"10.1016/j.ibiod.2025.106126","DOIUrl":"10.1016/j.ibiod.2025.106126","url":null,"abstract":"<div><div>Bioelectro-Fenton (BEF) systems have attracted attention for sustainable pollutant treatment, but the application of Fe/Cu-based cathodic catalysts remains limited by instability, metal leaching, and potential toxic by-products. The treatment of bisphenol A (BPA), a persistent and toxic endocrine disruptor, further challenges wastewater remediation. This study uses the response surface method to optimize the preparation conditions of Fe/Cu cathode catalysts for enhancing the performance of BEF systems by increasing H<sub>2</sub>O<sub>2</sub> generation, removing BPA, and decreasing the internal resistance of the cathode. The optimized Fe/Cu bimetallic catalyst applied to the BEF system exhibits 99.76 % removal of 10 mg/L BPA in 11 h, significantly outperforming the BEF with the monometallic catalysts (73.43 % and 58.51 % for Fe and Cu, respectively). The charge transfer resistance of the Fe/Cu catalyst is reduced by 61.79 %–63.09 % compared with that of the Fe and Cu catalysts. The optimized Fe/Cu catalyst is reused up to the eighth cycle with 73.34 % BPA removal efficiency, demonstrating its reuse potential. Microbial community analysis at the anode showed a marked shift, with <em>Pseudomonas</em> abundance increasing from 3.46 % to 51.76 %, suggesting that the optimized Fe/Cu catalyst promoted electroactive microbial enrichment and enhanced system performance.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106126"},"PeriodicalIF":4.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility assessment of magnetite for enhancing the clean utilization of lignite through anaerobic digestion 磁铁矿厌氧消化提高褐煤清洁利用的可行性评价

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-16 DOI: 10.1016/j.ibiod.2025.106122

Kai Zhang , Hongyu Guo , Norbert Klitzsch , Daping Xia , Zhazha Hu , Xiao Liu , Bin Zhang , Hao Chen

{"title":"Feasibility assessment of magnetite for enhancing the clean utilization of lignite through anaerobic digestion","authors":"Kai Zhang , Hongyu Guo , Norbert Klitzsch , Daping Xia , Zhazha Hu , Xiao Liu , Bin Zhang , Hao Chen","doi":"10.1016/j.ibiod.2025.106122","DOIUrl":"10.1016/j.ibiod.2025.106122","url":null,"abstract":"<div><div>This study pioneers the application of magnetite in anaerobic digestion of lignite, achieving dual enhancement of biomethane production and coal-derived waste valorization. At an optimal dosage of 2 g, magnetite increased cumulative methane yield by 55.4 % compared to the control, driven by selective enrichment of electroactive bacteria such as <em>norank_f_Synergistaceae</em> and <em>Proteiniclasticum</em>, alongside DIET-driven methanogens dominated by <em>Methanosaeta</em> at 86.29 % abundance. Concurrently, magnetite induced structural modification of lignite through degradation of recalcitrant aliphatic hydrocarbons and a 53.5 % increase in specific surface area from 7.772 to 11.924 m<sup>2</sup>/g, collectively improving the combustion efficiency of residual coal. These findings establish magnetite as a bifunctional catalyst that unlocks the bioenergy potential of low-rank coals while converting residual waste into cleaner solid fuels. The strategy offers coal-intensive regions a sustainable pathway to integrate biogas production with circular coal waste management.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106122"},"PeriodicalIF":4.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of 1,2-dichloroethane by Ancylobacter sp. BL1: Degradation characteristics, catabolic genes, and bioaugmentation in contaminated soil 双歧杆菌BL1对1,2-二氯乙烷的生物降解：在污染土壤中的降解特性、分解代谢基因和生物强化

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-12 DOI: 10.1016/j.ibiod.2025.106123

Zhiguo Wu , Lang Wang , Tian Qin , Shuxiu Sun , Xueyang Zhang , Zongzheng Yang

{"title":"Biodegradation of 1,2-dichloroethane by Ancylobacter sp. BL1: Degradation characteristics, catabolic genes, and bioaugmentation in contaminated soil","authors":"Zhiguo Wu , Lang Wang , Tian Qin , Shuxiu Sun , Xueyang Zhang , Zongzheng Yang","doi":"10.1016/j.ibiod.2025.106123","DOIUrl":"10.1016/j.ibiod.2025.106123","url":null,"abstract":"<div><div>1,2-dichloroethane (1,2-DCA) contamination has emerged as a notable threat to human health and ecological stability. However, studies on 1,2-DCA-contaminated soils are limited. Further, the remediation potential of bacterial genera capable of the aerobic degradation of 1,2-DCA and their use in the remediation of actual 1,2-DCA-contaminated soils have not yet been comprehensively investigated. To address these research gaps, in the present study, we isolated a novel 1,2-DCA-degrading bacterial strain from 1,2-DCA contaminated soil, identified as <em>Ancylobacter</em> sp. BL1 using 16 S rRNA gene analysis and physiological and biochemical tests. The optimal conditions for degradation by strain BL1 were predicted using response surface methodology, and the degradation kinetics was studied. The metabolic pathway of 1,2-DCA in strain BL1 follows a hydrolytic dehalogenation mechanism. The key genes (<em>dhlA</em>, <em>dhlB</em>, <em>max</em>, and <em>ald</em>) involved in 1,2-DCA degradation exhibited significant homology with previously reported genes except for <em>dhlB</em>, which was located on a large plasmid together with <em>dhlA</em>. To the best of our knowledge, for the first time, an efficient 1,2-DCA-degrading strain, BL1, was utilized for remediating actual 1,2-DCA-contaminated soil. Our findings demonstrated that strain BL1 effectively degraded approximately 86 % of 235 mg/kg of soil 1,2-DCA within 5 days. Bioaugmentation with the isolated strain did not considerably disturb the original microbial communities of the soil, increasing the abundances of microbial flora beneficial to organic carbon degradation and restoring soil enzyme activity. Our study will provide a foundation for the bioremediation of 1,2-DCA-polluted soil.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106123"},"PeriodicalIF":4.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metatranscriptomic profiling reveals microbial succession and adaptation strategies during long-term lignocellulose degradation 超转录组学分析揭示了长期木质纤维素降解过程中的微生物演替和适应策略

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-05-07 DOI: 10.1016/j.ibiod.2025.106110

Lucas Amoroso Lopes de Carvalho , Anna Carolina de Oliveira Souza , Camila Cesário Fernandes , Cleiton Dias do Prado , Eliana Gertrudes de Macedo Lemos , Lúcia Maria Carareto Alves , Daniel Guariz Pinheiro

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