International Biodeterioration & Biodegradation最新文献

Biotransformation and bioaccumulation of selenium by arbuscular mycorrhizal fungi associated with maize roots in natural seleniferous soils

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-18 DOI: 10.1016/j.ibiod.2025.106068

Tanveer Kaur , N. Tejo Prakash , M. Sudhakara Reddy

{"title":"Biotransformation and bioaccumulation of selenium by arbuscular mycorrhizal fungi associated with maize roots in natural seleniferous soils","authors":"Tanveer Kaur , N. Tejo Prakash , M. Sudhakara Reddy","doi":"10.1016/j.ibiod.2025.106068","DOIUrl":"10.1016/j.ibiod.2025.106068","url":null,"abstract":"<div><div>This study evaluated the role of arbuscular mycorrhizal fungi (AMF) isolated from naturally seleniferous soils in promoting plant growth, selenium (Se) uptake, and biotransformation in naturally selenium-contaminated fields. In AMF-inoculated plants, root biomass, shoot biomass, and maize cob biomass increased by 2.35-fold, 2.32-fold, and 1.93-fold, respectively, compared to control plants. Notably, AMF roots accumulated 7.80 % more selenium than control roots. However, selenium accumulation in the shoots and grains of AMF-inoculated plants was reduced by 71.83 % and 49.92 %, respectively. X-ray absorption near-edge spectroscopy revealed that toxic inorganic selenium species present in control plant roots were replaced by reduced elemental selenium in AMF-inoculated plants. Furthermore, AMF-inoculated plants exhibited higher levels of volatile organic selenium compounds, such as dimethyl selenide and dimethyldiselenide, which escaped from plant tissues, acting as a detoxification mechanism to mitigate selenium toxicity. To the best of our knowledge, this study is the first to report the role of indigenous AMF isolated from selenium-contaminated soils in facilitating selenium bioaccumulation and biotransformation to less toxic forms in plants under field conditions. The findings highlight that AMF not only enhance plant growth under selenium-stressed conditions by limiting selenium translocation to above-ground tissues but also protect plants by transforming selenium into methylated volatile derivatives. Thus, AMF protect the plants from Se toxicity by biotransformation to lesser toxic forms as well as enhancing plant growth activities. This approach can lead to successful utilization of seleniferous soils, which otherwise are considered not suitable for agricultural use.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106068"},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodeterioration of cultural heritage monuments: A review of their deterioration mechanisms and conservation

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-17 DOI: 10.1016/j.ibiod.2025.106066

Sangeeta Yadav , Diane Purchase

{"title":"Biodeterioration of cultural heritage monuments: A review of their deterioration mechanisms and conservation","authors":"Sangeeta Yadav , Diane Purchase","doi":"10.1016/j.ibiod.2025.106066","DOIUrl":"10.1016/j.ibiod.2025.106066","url":null,"abstract":"<div><div>Geochemical cycles result in the chemical, physical, and mineralogical modification of rocks, ultimately leading to the formation of soil. However, when stones and rocks form part of historic buildings and monuments, the effects are deleterious. In addition, microorganisms colonize these monuments over time, resulting in the formation of biofilms, while microbial metabolites cause physical weakening and discoloration of the stone. This process, known as biodeterioration, results in a significant loss of cultural heritage. Purification and 16S rDNA sequencing of bacteria growing on heritage monuments revealed that the most prominent taxa were closely related to <em>Bacillus</em> spp.<em>, Arthrobacter</em> spp., <em>Staphylococcus</em> spp. and <em>Paenibacillus</em> spp. In addition to bacteria, some fungal strains of the genera <em>Penicillium</em>, <em>Aspergillus</em>, <em>Fusarium</em> and <em>Alternaria</em> were also reported. To formulate effective conservation strategies to prevent biodeterioration and restore monuments, it is important to identify the microorganisms colonizing the substrate and the energy sources they utilize for sustenance. With this perspective, this review focuses on studies that explored the process of biodeterioration, the mechanisms by which microbes colonize and impact monuments, the techniques used to assess biodeterioration and conservation strategies designed to preserve the monuments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106066"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new control strategy of sulfate-reducing microorganisms and corrosion rate from a high-temperature oil reservoir by nitrate/nitrite coupling with nitrate-reducing bacteria

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-15 DOI: 10.1016/j.ibiod.2025.106065

Lei Zhou , Jie Gao , Yu-Xuan Li , Jun Wu , Biao Wang , Yi-Fan Liu , Shi-Zhong Yang , Ji-Dong Gu , Bo-Zhong Mu

{"title":"A new control strategy of sulfate-reducing microorganisms and corrosion rate from a high-temperature oil reservoir by nitrate/nitrite coupling with nitrate-reducing bacteria","authors":"Lei Zhou , Jie Gao , Yu-Xuan Li , Jun Wu , Biao Wang , Yi-Fan Liu , Shi-Zhong Yang , Ji-Dong Gu , Bo-Zhong Mu","doi":"10.1016/j.ibiod.2025.106065","DOIUrl":"10.1016/j.ibiod.2025.106065","url":null,"abstract":"<div><div>Different water-flooded oil reservoirs shape diverse compositions of sulfate-reducing microorganisms (SRM), and the activities of key SRM may involve different metabolic, corrosion and inhibition mechanisms. However, the current bioinhibition methods for SRM in various petroleum reservoirs are complex and variable, and universal and systematic model methods are lacking for field application. In the present study, the effective inhibition of SRM from the production water of the Jiangsu Oilfield was investigated using inhibitors and nitrate-reducing bacteria (NRB). The experiments, involving different concentrations of selective inorganic inhibitors, showed that nitrate and nitrite exhibited prominent inhibitory effects on the SRM community (JS) with sulfide reduction by 49.0%–62.5%, followed by molybdate and tungstate. With the target of the key SRM (<em>Archaeoglobus</em>) in the production water of the Jiangsu Oilfield, nitrate/nitrite and three NRB strains were evaluated for their inhibition performance. The nitrate-containing treatments were more related to the NRB species, whereas the nitrite-containing treatments were more related to nitrite concentrations. Comparatively, the sulfide inhibition of the nitrite-containing treatments was more significant, with an obvious influence on enzyme contents (Apr and Dsr). Two preferred inhibition combinations were obtained: nitrate (10 mmol/L) and ZW3; nitrite (5 mmol/L) and ZW3. They have been applied in the SRM community (JS) for effectiveness evaluation, with excellent performance in the control of sulfide production and corrosion rate. A nitrate-mediated model method for the bioinhibition of SRM in oil reservoirs is proposed. These results provide insights and strategies for microbial inhibition of SRM and corrosion control in oilfields.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106065"},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the dynamic changes of physicochemical properties, nitrogen functional genes, bacterial communities and their interactions during the composting of Chinese medicinal herbal residues

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-13 DOI: 10.1016/j.ibiod.2025.106063

Wanting Zheng , Lisheng Wang , Qiannuo Zeng, Jiayi Fu, Minxin Rao, Ying Zhang

{"title":"Study on the dynamic changes of physicochemical properties, nitrogen functional genes, bacterial communities and their interactions during the composting of Chinese medicinal herbal residues","authors":"Wanting Zheng , Lisheng Wang , Qiannuo Zeng, Jiayi Fu, Minxin Rao, Ying Zhang","doi":"10.1016/j.ibiod.2025.106063","DOIUrl":"10.1016/j.ibiod.2025.106063","url":null,"abstract":"<div><div>Aerobic composting of Chinese medicinal herbal residues (CMHRs) constitutes an efficacious waste management strategy, yet optimizing nitrogen content remains challenging. The variations of physicochemical properties, nitrogen functional genes, and bacterial communities throughout the composting process, along with their interrelationships were explored in this study. The final compost product achieved maturity, with a C/N ratio of 10.96 and a nitrification index of 0.19. Quantitative PCR analysis indicated suboptimal nitrification efficiency due to insufficient activity of nitrifying microorganisms. 52 biomarkers at four stages of composting were identified by linear discriminant analysis effect size. Functional annotation of prokaryotic taxa exposed chemoheterotrophy (17.05%–25.52%) and aerobic chemoheterotrophy (11.84%–22.65%) as dominant bacterial functions. Canonical correspondence analysis discovered total nitrogen exerted the most significant impact on bacterial genus distribution. Bacteria affectting nitrification genes and nitration products, especially <em>Anseongella</em> and <em>Weissella</em>, were spotted through network analysis. The results revealed the important microbial groups facilitating the nitrogen conversion of compost of CMHRs, which provided a basis for optimizing nitrogen retention and improving the quality of compost.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106063"},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of chicken feathers under oxygen-limited conditions: An alternative approach for nutrient recovery from feather waste 限氧条件下鸡毛的生物降解：从羽毛废物中回收营养物质的另一种方法

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-13 DOI: 10.1016/j.ibiod.2025.106064

Hai Nguyen , Hoang Le , Le Nguyen , Anh Do , Oanh Le , Hang Dinh

{"title":"Biodegradation of chicken feathers under oxygen-limited conditions: An alternative approach for nutrient recovery from feather waste","authors":"Hai Nguyen , Hoang Le , Le Nguyen , Anh Do , Oanh Le , Hang Dinh","doi":"10.1016/j.ibiod.2025.106064","DOIUrl":"10.1016/j.ibiod.2025.106064","url":null,"abstract":"<div><div>Poultry feathers are a challenging-to-degrade solid waste that requires proper management and treatment technologies. Conventionally, feather waste is treated by composting, which requires oxygen and takes a long time. Here, we present a method for degrading feather waste under oxygen-limited conditions, shortening the treatment time to 8 weeks while achieving a high decomposition efficiency of 81 %. The emission of sulfide odor was controlled as the degradation process was performed in closed chambers without aeration. The process produced feather lysate at a high yield of 0.9 L kg<sup>−1</sup>, retaining most of the nutrients from the feather waste. No PAHs, heavy metals, or pathogens were detected in the feather lysate, indicating a low risk for agricultural use. Feather lysate with a high keratinase content of 1330.13 U mL<sup>−1</sup> can be a source for obtaining this enzyme for various applications. The bacterial genera <em>Keratinibaculum</em> and <em>Bacillus</em> were identified as key drivers of the feather decomposition process.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106064"},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The biodegradation pathway of HBCDs in the enriched microbial community

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-12 DOI: 10.1016/j.ibiod.2025.106061

Bing Zhang , Wenqi Luo , Yongjin Liu , Haonan Chen , Tiantian Pan , Baole Wang , Keyan Li , Huaqing Jia , Shirou Zhou , Shanshan Meng , Xueying Ye , Tao Peng , Hui Wang , Ji-Dong Gu , Fei Yu , Zhong Hu

{"title":"The biodegradation pathway of HBCDs in the enriched microbial community","authors":"Bing Zhang , Wenqi Luo , Yongjin Liu , Haonan Chen , Tiantian Pan , Baole Wang , Keyan Li , Huaqing Jia , Shirou Zhou , Shanshan Meng , Xueying Ye , Tao Peng , Hui Wang , Ji-Dong Gu , Fei Yu , Zhong Hu","doi":"10.1016/j.ibiod.2025.106061","DOIUrl":"10.1016/j.ibiod.2025.106061","url":null,"abstract":"<div><div>1,2,5,6,9,10-Hexabromocyclododecanes (HBCDs) have been widely used in industrial applications, leading to serious environmental contamination. Previously, we reported that the biotransformation of HBCDs in the enriched microbial communities might involve the interactions between degraders and other taxa. In the present study, we further investigate this phenomenon in these communities. We found that all microbial communities acquired HBCDs degradation capacity at the 24th transfer, with degradation rates ranging from 12.8% to 55.9%. The degradation of HBCDs was coupled with the growth of culturable bacteria in the communities. <em>Alcanivorax</em> (81.14%, relative abundance) maintained its dominance in the microbial community SZH2-24. Moreover, metatranscriptomic analysis revealed that <em>dadAH</em> and <em>dadBH</em> were the only dehalogenase genes induced by HBCDs. Functional genes of both <em>Alcanivorax</em> and other taxa were potentially involved in the downstream biodegradation pathway. Debrominated products were likely oxidized, hydrolyzed, and ring-cleaved by phenol 2-monooxygenase (<em>Alcanivorax</em>), toluene methyl-monooxygenase electron transfer component (<em>Hyphomicrobium</em>), quercetin 2,3-dioxygenase (<em>Hyphomicrobium</em> and <em>Alcanivorax</em>), and 4,5-DOPA dioxygenase extradiol (<em>Alcanivorax</em>). Subsequently, the ring-cleavage products were probably degraded via Fatty acid metabolism (Hyphomicrobiales, <em>Alcanivorax</em>, and <em>Hyphomicrobium</em>) rather than TCA cycle. <em>Alcanivorax</em> harbored a complete degradation pathway, which might have provided a competitive advantage over other taxa. In contrast, minor taxa might degrade downstream products.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106061"},"PeriodicalIF":4.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaerobic reductive dechlorination of chlorobenzenes by an enrichment culture containing Dehalobacter species

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-11 DOI: 10.1016/j.ibiod.2025.106062

Shaohua Cao , Dexuan Tang , Yang Tian , Lianghua Lu , Wenjing Qiao , Tao Long , Jiandong Jiang

{"title":"Anaerobic reductive dechlorination of chlorobenzenes by an enrichment culture containing Dehalobacter species","authors":"Shaohua Cao , Dexuan Tang , Yang Tian , Lianghua Lu , Wenjing Qiao , Tao Long , Jiandong Jiang","doi":"10.1016/j.ibiod.2025.106062","DOIUrl":"10.1016/j.ibiod.2025.106062","url":null,"abstract":"<div><div>Chlorinated benzenes are pervasive contaminants in soil and groundwater at industrial sites globally. We previously obtained an anaerobic 1,2,4-trichlorobenzene-dechlorinating enrichment culture originated from a chemical plant. In this study, we evaluated the dehalogenation capability of this consortium to utilize all chlorobenzene congeners as the ultimate electron acceptors. Over a period of approximately 250 days’ incubation, 11 out of 12 chlorobenzene congeners were successfully dechlorinated to monochlorobenzene, suggesting that the consortium has no preferential dehalogenation pattern for flanked chlorines on the benzene ring. Distinct dechlorination pathways strongly suggested that novel dechlorinator and reductive dehalogenases prevailed in this consortium. The 16S rRNA gene amplicon sequencing revealed that the microbial communities were predominantly composed of fermenters such as <em>Sporomusa</em>, <em>Desulfitobacterium</em> and <em>Longilinea</em>; methanogenic <em>Methanobacterium</em>; and the organohalide-respiring bacterium <em>Dehalobacter</em>. The bottles actively engaged in chlorobenzene dechlorination harbored higher abundances of <em>Dehalobacter</em> species. Quantitative PCR analysis further demonstrated that <em>Dehalobacter</em> conserved energy from chlorobenzene dechlorination, with growth yields ranging from 1.2 × 10<sup>5</sup> to 2.27 × 10<sup>7</sup> copies per μmol of Cl<sup>−</sup> released. The versatile chlorobenzene dechlorination capacities of the consortium underscores its significant potential for remediating chlorobenzene-contaminated field sites.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106062"},"PeriodicalIF":4.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low aeration rate during the cooling phase facilitated the removal of antibiotic resistance genes in the sludge and straw compost

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-08 DOI: 10.1016/j.ibiod.2025.106047

Bing Wang , Xu Bao , Jiawei Li , Weizhen Ma , Guomin Li , Jian Zhang , Hongxin Lv

{"title":"Low aeration rate during the cooling phase facilitated the removal of antibiotic resistance genes in the sludge and straw compost","authors":"Bing Wang , Xu Bao , Jiawei Li , Weizhen Ma , Guomin Li , Jian Zhang , Hongxin Lv","doi":"10.1016/j.ibiod.2025.106047","DOIUrl":"10.1016/j.ibiod.2025.106047","url":null,"abstract":"<div><div>To evaluate the effect of aeration rate on microorganisms and antibiotic resistance genes (ARGs) in an aerobic composting system, co-composting experiments with varying aeration rates were conducted during the high-temperature and cooling phases of composting. The results showed that the content of ARGs in all treatment groups decreased by 1.2–4.2 logs at the end of composting. Furthermore, the low aeration rate treatment during the cooling phase (CAno group) was highly effective in reducing the risk of ARG dissemination. Compared with the control group, ten ARGs decreased by 0.04–1.13 logs, except for <em>sul2</em>, which increased by 0.02 logs. In the CAno group, denitrification slightly increased. Several ARGs associated with bacterial genera were evaluated as potential hosts for ARGs, such as <em>Unspecified_Pseudomonadaceae</em> and <em>Acholeplasma</em>. The CAno group exhibited the lowest abundance of these bacteria, contributing further to the reduction of ARGs in compost.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106047"},"PeriodicalIF":4.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced biodegradation of phenol using immobilized Pseudomonas alloputida BF04 in sequencing batch reactor

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-07 DOI: 10.1016/j.ibiod.2025.106030

Lei Zou , Nihong Yao , Jinyan Li , Shuyi Yang , Wenli Zhou , Jieke Sheng , Zhijie Li , Qiaoyun Huang , Wenli Chen

{"title":"Enhanced biodegradation of phenol using immobilized Pseudomonas alloputida BF04 in sequencing batch reactor","authors":"Lei Zou , Nihong Yao , Jinyan Li , Shuyi Yang , Wenli Zhou , Jieke Sheng , Zhijie Li , Qiaoyun Huang , Wenli Chen","doi":"10.1016/j.ibiod.2025.106030","DOIUrl":"10.1016/j.ibiod.2025.106030","url":null,"abstract":"<div><div>Phenol, a prevalent and persistent organic contaminant in industrial effluents, is widely recognized for its susceptibility to biodegradation as an effective remediation strategy. In this study, the phenol-degrading bacterium <em>Pseudomonas alloputida</em> BF04 was isolated from petrochemical effluent and exhibited the capacity to degrade 500 mg/L of phenol within 30 h. Immobilizing strain BF04 in polyvinyl alcohol-sodium alginate (PVA-SA) beads markedly improved its tolerance to phenol and degradation efficiency. Batch reactor experiments employing immobilized beads achieved complete phenol removal under simulated wastewater conditions, sustaining a phenol degradation efficiency of 100%. Furthermore, the reactor exhibited significant removal efficiencies for Chemical Oxygen Demand (COD, 77%), Total Nitrogen (TN, 84%), and Ammonium (NH<sub>4</sub><sup>+</sup>, 82%) when the hydraulic retention time (HRT) was maintained above 24 h. Functional gene sequencing identified the <em>dmp, Cat</em>, and <em>pca</em> gene clusters in strain BF04, suggesting that phenol degradation occurs via the meta-cleavage pathway of aromatic ring opening. The results demonstrated that the immobilized <em>Pseudomonas alloputida</em> BF04 strain exhibited excellent degradation ability and stability, effectively addressing challenges in practical wastewater treatment. This study provides a valuable reference for advancing bioremediation technology.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106030"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous glucose as the potential energy fuel stimulates the microcystin-LR degradation of Sphingosinicella microcystinivorans Y2

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2025-03-06 DOI: 10.1016/j.ibiod.2025.106051

Yuzhu Peng , Jiahui Liu , Xinger Xie , Qibai Tian , Shuijiao Chen , Xian Zhang

{"title":"Exogenous glucose as the potential energy fuel stimulates the microcystin-LR degradation of Sphingosinicella microcystinivorans Y2","authors":"Yuzhu Peng , Jiahui Liu , Xinger Xie , Qibai Tian , Shuijiao Chen , Xian Zhang","doi":"10.1016/j.ibiod.2025.106051","DOIUrl":"10.1016/j.ibiod.2025.106051","url":null,"abstract":"<div><div>External carbon sources have been recognized to affect the microbial-mediated remediation of microcystin (MC) in both natural and industrial polluted water bodies; however, the underlying molecular mechanisms remain unclear but would be worthy of further study. In this case, the MC-degrading bacterium, <em>Sphingosinicella microcystinivorans</em> Y2, was used to investigate the effect of exogenous glucose on MC biodegradation and its potential regulatory mechanisms. Our phenotypic results indicated that external glucose, within specific concentration ranges (125, 500, and 1000 mg/L), substantially accelerated the efficiency of MC degradation. Additionally, morphological observations suggested that glucose may positively impact the bacterial cells. A genome-oriented analysis of <em>S</em>. <em>microcystinivorans</em> Y2 was conducted to identify several Mlr-dependent MC-degrading genes, which were involved in the complete degradation of MC. Enrichment analysis of differentially expressed genes further indicated that glucose-promoted MC biodegradation may be associated with various biological processes. Furthermore, the gene-gene interaction network revealed the co-expression of genes <em>mlrA</em> and <em>nadD</em>, suggesting that increased levels of NAD<sup>+</sup> may positively stimulate various biochemical reactions to fuel the MC degradation of strain Y2. In short, our findings present that exogenous glucose might accelerate the bacterial-mediated MC biodegradation, and further reveal its underlying molecular mechanisms, which provides the theoretical basis for the application of external carbon in the microbial treatment of toxic MC.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106051"},"PeriodicalIF":4.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0