International Biodeterioration & Biodegradation最新文献

{"title":"Integrated transcriptomic and proteomic analysis of nicotine metabolism in Paenarthrobacter nicotinovorans ATCC 49919","authors":"Amada El-Sabeh,&nbsp;Andreea-Mihaela Mlesnita,&nbsp;Marius Mihasan","doi":"10.1016/j.ibiod.2025.106017","DOIUrl":"10.1016/j.ibiod.2025.106017","url":null,"abstract":"<div><div>The global tobacco industry produces significant amounts of nicotine-containing wastes, being regarded as a major environmental threat. The appropriate treatment of nicotine-contaminated waste is required for the removal of the toxic alkaloid before safe disposal. In this context, nicotine-degrading microorganisms and their enzymatic apparatus are of major interest for decontaminating and repurposing nicotine waste. Here, by combining long-read direct RNA sequencing data and nanoLC-MS/MS based proteomics data, we focused on the multiomic characterisation of the bacterial nicotine catabolic process from <em>Paenarthrobacter nicotinovorans</em> ATCC 49919. The nicotine-related expression of 25 annotated <em>nic</em>-genes and proteins was confirmed, eight of these genes (<em>JMY29_20530</em>, <em>JMY29_20550</em>, <em>perm</em>, <em>coxF</em>, <em>coxE</em>, <em>JMY29_20640</em>, <em>JMY29_20655</em>, <em>modB</em>) being reported here first as having nicotine-related expression. Insights regarding the active mechanisms involved in integrating the nicotine catabolic pathway with the general metabolism of the bacterial cell and the defence systems employed against the oxidative stress generated during nicotine degradation are also presented. This study provides the first multiomic investigation of <em>P. nicotinovorans</em> ATCC 49919 and, moreover, the first multiomic assessment of a bacterial nicotine catabolic pathway.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"199 ","pages":"Article 106017"},"PeriodicalIF":4.1,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing cadmium tolerance and photosynthesis in Cosmos bipinnatus Cav. By hormone methyl jasmonate","authors":"Chunyu Fan ,&nbsp;Xiaofang Yu ,&nbsp;Liu Yang ,&nbsp;Linjie Yue ,&nbsp;Xiaoxuan Zeng ,&nbsp;Yujia Liu ,&nbsp;Xue Xiao ,&nbsp;Lijuan Yang ,&nbsp;Fuwen Luo","doi":"10.1016/j.ibiod.2024.105976","DOIUrl":"10.1016/j.ibiod.2024.105976","url":null,"abstract":"<div><div><em>Cosmos bipinnatus</em> Cav. (<em>C. bipinnatus</em>), a highly cadmium (Cd)-tolerant landscape plant, shows promise for Cd pollution remediation. This study investigates the role of methyl jasmonate (MeJA) in enhancing Cd tolerance under high Cd stress (40, 120 μM) by regulating endogenous hormones. Cd stress severely impacts <em>C. bipinnatus</em>, reducing fresh/dry weight by 44.49%–83.81%, alongside declines in chlorophyll content and photosynthetic efficiency (F_v/F_m). However, low-concentration MeJA (0.1 μM) improves Cd tolerance by reducing water content by 3.09%–22.43%, increasing biomass by 12.82%–52.73%, decreasing Cd uptake by 4.56%–5.64%, and restoring chlorophyll synthesis and F_v/F_m. MeJA also modulates non-protein thiols (NPTs), especially decreasing NPTs in leaves under Cd stress and enhances glutathione conversion to phytochelatins, critical for detoxification. Endogenously, MeJA triggers rapid accumulation of jasmonic acid, abscisic acid (ABA), and indole-3-acetic acid while reducing gibberellin, which can be harmful to photosynthesis under Cd stress. MeJA also prevents prolonged ABA accumulation, mitigating its levels, thus protecting the photosynthetic system. These findings highlight MeJA's regulatory effects on hormone balance and suggest new approaches for effective soil Cd pollution remediation through enhanced plant tolerance mechanisms.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105976"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar's multifaceted role in bioremediation of emerging contaminants and heavy metals in complex rhizospheric ecosystem","authors":"Shiv Vendra Singh ,&nbsp;Shivangi Raghuvanshi ,&nbsp;Yogeshwar Singh ,&nbsp;Krishna Kumar Yadav ,&nbsp;Amel Gacem ,&nbsp;Tony Manoj K. Nandipamu ,&nbsp;Mohammad Khalid ,&nbsp;Rashida Hameed ,&nbsp;Rashmi Sharma ,&nbsp;Debarati Datta ,&nbsp;Saurabh Ghosh ,&nbsp;Arpna Kumari ,&nbsp;Ajay Kumar Singh ,&nbsp;Biswajit Pramanick ,&nbsp;Xiuxiu Zhang ,&nbsp;Chongqing Wang ,&nbsp;Maha A. Alreshidi","doi":"10.1016/j.ibiod.2025.106005","DOIUrl":"10.1016/j.ibiod.2025.106005","url":null,"abstract":"<div><div>Rising prevalence of emerging contaminants (ECs) and priority heavy metals (PHMs) poses grave threats to the health of the environment and humankind, majorly resulting from human activity such as mining, disposal of industrial wastes, and use of chemicals. These pollutants drastically reduce soil biodiversity, fertility, and crop yield, rendering agricultural goods hazardous. Biochar has recently received attention as a sustainable bioremediation solution for ECs and PHMs through diverse physical, chemical, and biological processes. Biochar has demonstrated significant bioremediation efficiency for PAHs, antibiotics, microplastics, and pesticides varied from 50 to 95% and 60–90% for PHMs in a wide range of ecosystems. The interactive mechanisms of complexation, precipitation, ion exchange, surface sorption, and electrostatic interaction, hydrophobic interaction electron donor and acceptor interaction altogether enhance contaminant immobilization and biodegradation. Furthermore, biochar has been shown to aid in the breakdown of contaminants while lowering the transportation and accessibility of heavy metals. Besides remediation, biochar improves the rhizospheric environment by enhancing plant growth, nutrient uptake, and soil vitality. Its ability to remove both heavy metals and organic pollutants from wastewater and soil matrices, and its influence on their bioavailability and transport, show the dual nature of biochar in restoring environments. This manuscript attempts to provide in-depth insight into the challenges that ECs and PHMs pose, the role of biochar in their removal, and delicate soil-plant-biochar interactions. The work here discusses these interacting effects, thus giving insight into the potential of biochar in the immobilization of ECs and PHMs through many interspecific reactions, and also the soil-plant-biochar interactions and possibilities for successful remediation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106005"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient removal performance and microbial community analysis in an integrated wastewater treatment system with macroalgae (U. meridionalis) and Bacillus","authors":"Junxian Li ,&nbsp;Zhongming Zheng ,&nbsp;Yangcai Wang ,&nbsp;Betina Lukwambe ,&nbsp;Zhao Yang","doi":"10.1016/j.ibiod.2025.106006","DOIUrl":"10.1016/j.ibiod.2025.106006","url":null,"abstract":"<div><div>The integrated aquaculture wastewater treatment system has been widely applied and typically consists of multiple units, among which macroalgae are critical for nutrients removal. This study investigated the impact of the addition of <em>Bacillus</em> to the macroalgae unit on the nutrient removal performance of the system and its mechanisms by analyzing the bacterial community that was identified through high-throughput sequencing of 16S rRNA. The results showed that <em>Bacillus</em> significantly influenced the structural composition of the bacterial communities both in the water and on the algal surface within the macroalgae unit. The enrichment of bacteria promoted nitrogen cycling and algal growth, such as <em>Algoriphagus</em> and <em>Marivita</em>. Futhermore,a higher average variation degree was observed, suggesting forming a more stable bacterial community. The removal rates of nitrogenous nutrients, such as TAN, NO₃⁻-N, and NO₂⁻-N, improved, reaching 99.92%, 99.62%, and 99.70%, respectively. Additionally, the biomass of the algae (<em>U. meridionalis</em>) significantly improved, reaching 157%. The <em>Bacillus</em> also increased the contribution of stochastic processes (drift) in the assembly of the bacterial communities in both the water and on the algal surface. This study provides foundational support for optimizing integrated wastewater treatment systems.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106006"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streamlining automation in a rapid wood durability test against the marine wood boring crustacean, Limnoria quadripunctata.","authors":"L.S. Martin ,&nbsp;S.M. Cragg ,&nbsp;K.W. Kot ,&nbsp;J.R. Shipway","doi":"10.1016/j.ibiod.2024.105993","DOIUrl":"10.1016/j.ibiod.2024.105993","url":null,"abstract":"<div><div>Gribble significantly impact the biodegradation of wood in marine environments, causing costly damages, but can be used to easily assess wood consumption rates. Faecal pellet production indicates feeding rate and is a good proxy for degradation. However, counting large numbers of pellets by eye may be impractical with multiple replicates. Optimising a rapid, cost-effective, and user-friendly protocol to evaluate wood durability against gribble can be used in development of sustainable wood protection methods. Automated counting methods ImageJ and FlowCAM, were evaluated against manual counts for efficiency and accuracy. ImageJ was more suitable for large sample volumes, offering a balance of cost, time, and accuracy. Both methods showed greater degrees of error as faecal pellet counts increased. ImageJ tended to undercount, whereas FlowCAM often overcounted significantly. The FlowCAM rapidly processed individual samples, however loading/unloading samples increased time-consumption as replicates increased. ImageJ was able to process multiple samples at once so total time was not influenced by sample size. This research underlines the importance of optimising counting methods to accurately assess the impact of gribble on wood degradation, offering a streamlined approach for both specialist and non-specialist laboratories to evaluate wood durability and enhance the development of non-toxic, sustainable wood protection solutions.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105993"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of organic loading rate on the cultivation and stability of aerobic granular sludge in continuous flow reactor","authors":"Yingjian Zhang ,&nbsp;Fanchao Meng ,&nbsp;Zehan Huang ,&nbsp;Minghui Liu ,&nbsp;Xuegang Mu ,&nbsp;Xuelong Zhang ,&nbsp;Guilherme Lelis Giglio ,&nbsp;Zhaoxu Peng","doi":"10.1016/j.ibiod.2025.106016","DOIUrl":"10.1016/j.ibiod.2025.106016","url":null,"abstract":"<div><div>To investigate how organic loading rate (OLR) affects the formation of aerobic granular sludge (AGS) in continuous flow system, the effect of OLR adjusted by hydraulic retention time (HRT) and influent chemical oxygen demand (COD) on pollutant removal, granulation efficiency and microbial community were comprehensively analyzed. The results showed that increasing OLR by decreasing HRT led to significant filamentous sludge bulking and biomass loss. Conversely, gradually increasing the OLR to 0.19 kg·(kg·d)⁻¹ by adjusting influent COD improved the granulation efficiency from 3.00% to 28.40% with the average particle size of 193.60 μm at the 60th day. However, excessive OLR would promote filamentous bacteria to use the residual COD in the oxic tank. Organic matter utilization path analysis showed that the COD utilization enhanced by phosphate accumulating organisms (PAOs), glycogen accumulating organisms (GAOs) and adsorption (44.85 ± 2.50%) during anaerobic phase when influent COD was 350 mg L⁻¹. And the relative abundance of PAOs and GAOs increased to 4.14% and 3.18%, respectively. This was the main cause of granulation. Furthermore, strategies to enhance granulation in continuous flow systems were proposed: Increase the volume of anaerobic tank or introduce intermittent mixing to keep more influent organics is used by bigger biomass before entering the oxic tank.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106016"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periphyton biofilms formulation and application for the removal of trace pollutants from water","authors":"Khurram Shahzad ,&nbsp;Shahid Mahmood ,&nbsp;Azeem Khalid ,&nbsp;Rai Muhammad Amir ,&nbsp;Rab Nawaz ,&nbsp;Marlia Mohd Hanfiah ,&nbsp;Zaini Bin Sakawi ,&nbsp;Muzammil Anjum","doi":"10.1016/j.ibiod.2025.106003","DOIUrl":"10.1016/j.ibiod.2025.106003","url":null,"abstract":"<div><div>Freshwater resource contamination with trace metals (TMs) poses a major risk to public health and the ecosystem. In the current study, three distinct types of periphyton biofilms (PPBFs)—epiphyton, epilithon, and metaphyton—were cultured and employed to remove TMs from the simulated river water. The PPBFs were isolated from freshwater and then cultured in Bio-carrier of Organic Natural Aquatic Mate (BONAM). Based SEM analysis, the periphyton community appears porous and filamentous, with microscopic pores, unique individual cells, and extracellular matrix. Even while each PPBF has a unique structure and function, they all share rich active surface functional groups, particularly carboxylic groups. The epiphyton was found to be more effective for the removal of TMs when tested at 20 mg/L of the TMs as evidenced by the 79.50% elimination of arsenic (As) following 144 h of treatment. The removal performance of epiphyton decreased to just 57.40 and 51.60% at TMs concentration of 40 and 60 mg/L, respectively. Temperature had a significant impact on the biosorption of TMs utilising epiphyton, as evidenced by the removal efficiency of 75.05% at 15 °C, which decreased to 69.50 and 61.00% at 25 °C and 35 °C, respectively. The findings pertaining to the removal of TMs most accurately represented pseudo-first-order kinetics, indicating the bio-absorption of the TMs into the PPBFs. The elimination of almost 92% of TMs under optimal conditions of 90 h of incubation at neutral pH, 25 °C, 1.0 g L⁻¹ of biomass, and 20 mg/L of TMs concentration demonstrated that epiphyton was the most effective biomaterial.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106003"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfadiazine degradation by Acinetobacter sp. strain H-3 and its applicability in soil at low temperatures","authors":"Shuang Zhao ,&nbsp;Yi Zhang ,&nbsp;Rongjiu Shi ,&nbsp;Xiaolong Liang ,&nbsp;Ping Li ,&nbsp;Xue Bai ,&nbsp;Siqin Han ,&nbsp;Ying Zhang","doi":"10.1016/j.ibiod.2025.105998","DOIUrl":"10.1016/j.ibiod.2025.105998","url":null,"abstract":"<div><div>The prolonged and widespread use of veterinary antibiotics resulted in a significant accumulation of antibiotic residues in the soil surrounding poultry farms, thereby promoting the proliferation and dissemination of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB). In the northern regions of China, low temperatures hinder the microbial degradation of antibiotics. This study reports that <em>Acinetobacter</em> sp. H-3, isolated from a poultry farm in Liaoning Province, Northeast China, is capable of effectively degrading SDZ at a minimum temperature of 5°C, with optimal degradation efficiency observed at 15°C. The strain H-3 removed 82% of SDZ from the soil over a 14-day period at 15°C, with 53% of this degradation directly attributed to the activity of strain H-3. High-throughput sequencing and RT-qPCR analyses revealed that exposure to SDZ significantly altered the soil bacterial community structure, inhibited soil microbial functions and increased the abundance of <em>sul1</em>, <em>sul2</em> and <em>intI1</em> genes. Following the application of, certain bacterial genera in the contaminated soil exhibited indications of recovery. However, the overall soil microbial function did not show a recovery trend. Additionally, the presence of strain H-3, which carries the <em>sul1</em>, <em>sul2</em> and <em>intI1</em> genes, resulted in an increase in <em>sul1</em>, followed by a subsequent decrease, while the levels of <em>sul2</em> and <em>intI1</em> continued to rise. This study is the first to report that <em>Acinetobacter</em> sp. strain H-3 can effectively remove SDZ at low temperatures. Furthermore, it provides a preliminary evaluation of the ecological risks linked to the dissemination of ARGs during the application of this strain, contributing valuable resources and theoretical insights for the bioremediation of antibiotic-polluted soil in cold areas.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105998"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of an efficient chloroxylenol-degrading bacterial consortium","authors":"Lu Xu ,&nbsp;Yinhu Jiang ,&nbsp;Jiale Ma ,&nbsp;Kexin Wang ,&nbsp;Ying Zhou ,&nbsp;Qimiao Xu ,&nbsp;Jiguo Qiu ,&nbsp;Jiandong Jiang ,&nbsp;Jian He ,&nbsp;Zhuang Ke","doi":"10.1016/j.ibiod.2025.105999","DOIUrl":"10.1016/j.ibiod.2025.105999","url":null,"abstract":"<div><div>Chloroxylenol (synonym, para-chloro-meta-xylenol [PCMX]), a halogenated phenolic disinfectant, is widely used for disinfection or in personal care products. Due to the global COVID-19 pandemic, the widespread use of PCMX has undoubtedly led to its prevalence in various environments, resulting in a high detection frequency. However, there have been few reports on the aerobic microbial community and pure culture degradation of PCMX. In this study, a PC2 consortium with an efficient PCMX decomposition was successfully obtained. <em>Rhodococcus</em> was significantly enriched in consortium PC2 after the acclimation. Subsequently, a PCMX-degrading pure culture strain, <em>Rhodococcus</em> sp. JH-7, was isolated, and the degradation characteristics were investigated. Meanwhile, a key metabolic intermediate (4-chloro-3,5-dimethylcatechol) was identified by LC-TOF-MS and a new biodegradation pathway of PCMX was proposed. Additionally, <em>Rhodococcus</em> sp. JH-7 could protect <em>Escherichia coli</em>, <em>Pichia pastoris</em>, <em>Chlorella ellipsoidea</em> and <em>Chlamydomonas reinhardtii</em> from the toxicity of PCMX, indicating that the biodegradation of PCMX was a detoxification process. This study reveals a catabolic pathway of PCMX and provides new insights for the bioremediation of PCMX-contaminated environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105999"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment of methanotrophic consortium and its aerobic cometabolism of mixed vinyl chloride pollutants using methane gas and micro- and nano-bubbles","authors":"Chun-Chin Wang ,&nbsp;Chih-Ming Liang ,&nbsp;Ting-I Lin ,&nbsp;Chu-Fang Yang","doi":"10.1016/j.ibiod.2025.106011","DOIUrl":"10.1016/j.ibiod.2025.106011","url":null,"abstract":"<div><div>Chlorinated ethylenes (CEs) are widely used in various industries, making them common groundwater pollutants. The reductive dechlorination applied to deal with CEs has the drawback of lower-chlorinated CEs accumulation. Aerobic co-metabolism relying on methanotrophs is an attractive strategy to deal with CEs because of its fast CE degrading rate and no lower-chlorinated CEs accumulation. In this study, one methanotrophic consortium was acclimated with methane and TCE concentrations for five phases. The bacterial community and methane monooxygenase (MMO) functional genes were analyzed during enrichment. The methane gas and micro-nano bubbles (MNBs) were applied to perform batch experiments using the acclimated methanotrophic consortium as the inoculum. The results indicated that a methanotrophic consortium M-5%-0.2 was successfully acclimated. Genus <em>Methylocystis</em> was dominant in the acclimated consortium M-5%-0.2, and the particulate methane monooxygenase gene (<em>pmoA</em>) could be detected. With different CEs combinations and 5% methane gas, 0.2 and 0.3 mg/L cis-1,2-DCE and VC could be entirely removed. TCE removal efficiencies were 38.8% and 40.2%, as the TCE concentrations were 0.2 and 0.3 mg/L, respectively. CEs removal pattern was similar in the presence of various methane MNBs ratios. The priority order of CEs removal was VC &gt; cis-1,2-DCE &gt; TCE. The degradation efficiencies of 0.2 mg/L TCE, cis-1,2-DCE, and VC were 17.4%, 78.4%, and 100% when adding 24% methane MNBs water as the carbon source. The methanotrophic consortium M-5%-0.2 could utilize the methane MNBs having a diameter larger than 235 nm. In conclusion, the aerobic co-metabolism strategy using methane MNBs has the potential to be applied for CEs bioremediation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106011"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}