International Biodeterioration & Biodegradation最新文献

{"title":"Optimizing biological pretreatment and bioleaching processes in sewage sludge: Heavy metal removal mechanisms and EPS-dependent dewaterability enhancement","authors":"Xiaochun Wang ,&nbsp;Jun Li ,&nbsp;Zemiao Shi ,&nbsp;Xiangtong Zhou ,&nbsp;Shanwei Li ,&nbsp;Xiaolei Zhang","doi":"10.1016/j.ibiod.2026.106324","DOIUrl":"10.1016/j.ibiod.2026.106324","url":null,"abstract":"<div><div>To overcome the inhibitory effect of organic matter on sludge bioleaching, this study systematically evaluated the impacts of two pretreatment methods (aeration and anaerobic digestion) on subsequent bioleaching performance. Results demonstrated that aeration pretreatment significantly accelerated the acidification process, enhanced overall heavy metals (HMs) removal efficiency, and effectively minimized phosphorus loss. Following the selection of aeration pretreatment, three bioleaching processes (direct, aerated, and anaerobic bioleaching) were compared. Aerated bioleaching achieved the fastest acidification (pH 2.08 in 6 days) and optimal removal of Cu²⁺, Zn²⁺, and Pb²⁺. Microbial community analysis revealed that the bioleaching process greatly changed community abundance and diversity, while particularly enriching key bioleaching genera such as <em>Acinethiobacillus</em> and <em>Alicyclobacillus</em>. This evolution was closely associated with the selectivity of HMs removal. All bioleaching processes achieved effective sludge reduction (MLSS decreased by 26.99% - 63.21%) and improved dewaterability. Aerated bioleaching yielded the optimal dewatering performance (specific resistance to filtration, SRF: 1.24 × 10¹³ m/kg), attributed to its induced reorganization of extracellular polymeric substances (EPS)—characterized by protein enrichment in tightly bound EPS and polysaccharide stripping from loosely bound EPS. This study provides critical theoretical insights for optimizing sludge bioleaching parameters and pretreatment-process combinations to maximize HM removal while minimizing nutrient loss.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"211 ","pages":"Article 106324"},"PeriodicalIF":4.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial colonization on biocide-treated and untreated façades: The impact of surface orientation","authors":"Michał Ciok ,&nbsp;Daniela Weisenberger ,&nbsp;Moritz Nichterlein ,&nbsp;Stefan Kalkhof ,&nbsp;Matthias Noll","doi":"10.1016/j.ibiod.2026.106311","DOIUrl":"10.1016/j.ibiod.2026.106311","url":null,"abstract":"<div><div>Biocides such as Octylisothiazolinone (OIT) and Terbutryn (TER) are commonly used in building materials to prevent microbial infestation. Their release and antimicrobial efficiency are influenced by wind-driven rain (WDR), which is directly related to façade orientation. This study investigates the bacterial and fungal community composition on biocide-free (BFF) and biocide-containing (BCF) façades with N/W (WDR) or S/E orientation, representing contrasting microclimatic conditions in terms of solar radiation, drying rates, and moisture availability. Sampling was carreid out after one year of outdoor exposure using a cultivation-independent approach. Bacterial and fungal community composition significantly differed between BCF and BFF. Furthermore, the fungal and bacterial community composition was significantly affected by façade orientation. Façades with BCFs S/E showed significantly higher bacterial richness than other façades. Moreover, members of the genera <em>Nesterenkonia, Paracoccus, Microbacteriaceae, Pseudomonas,</em> and <em>Dioszegia</em> dominated BFFs N/W and BFFs S/E, whereas <em>Klenkia</em>, <em>Massilia</em>, <em>Capnodiales</em>, and <em>Filobasidium</em>, were mostly present on BCFs N/W. Only the fungal genus <em>Vishniacozyma</em> was present in every treatment. Bacterial and fungal genera affiliated to biodeterioration, discoloration, and biodegradation, such as <em>Nocardioides</em>, <em>Sphingomonas,</em> Kineosporiaceae, and <em>Arthrobacter</em> were mainly found on BCFs N/W, <em>Arthrobacter</em> was additionally observed on BFFs N/W, and fungal genera <em>Cladosporium</em> and <em>Alternaria</em> were frequently present on BFFs S/E and BFFs N/W. These results demonstrate selective pressure on the microbial colonization patterns by both the presence of biocides and façade orientation. Therefore, these findings provide a broader perspective on the façade microbiome and suggest the need to adjust biocide usage to redefine methods, including considerations of weathering side and biocide application.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106311"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autotrophic denitrification filter prepared using gasification coarse slag and pyrite: Physicochemical characteristics and performance removal of nitrogen","authors":"Shuhan Yang ,&nbsp;Defu Xu ,&nbsp;Jing Su ,&nbsp;Zhengxuan Li ,&nbsp;Alan Howard","doi":"10.1016/j.ibiod.2026.106312","DOIUrl":"10.1016/j.ibiod.2026.106312","url":null,"abstract":"<div><div>Conventional heterotrophic denitrification processes face challenges in achieving the requirements of sustainable development goals due to their dependence on external organic carbon sources and the risk of secondary pollution. In this study, the nitrogen removal performance of a sulfur autotrophic denitrification filter prepared from gasification coarse slag (GCS) and pyrite was evaluated, and its underlying mechanisms were elucidated using microbial community, EDS-SEM, BET, and FT-IR analyses. Pyrite and GCS were combined at a 4:6 mass ratio, followed by the addition of Silica fume (7%), NaOH (4%), and Portland cement (10%) all expressed as percentages relative to the total mass of GCS and pyrite mass). The resulting mixture was granulated to form 10-mm GCS-PRFM particles and steam-cured at 80 °C. The GCS-PRFM exhibited a compressive strength of 3.0 MPa, bulk density of 1272 kg/m³, water absorption rate of 13.5%, specific surface area of 17.00 m²/g, and total pore volume of 0.048 cm³/g. Its specific surface area and total pore volume were 10.3-fold and 16-fold greater, respectively, than those of the pyrite filter (PRFM). The nitrate removal efficiency of GCS-PRFM (81.23%) was approximately double that of PRFM (40.92%). GCS-PRFM supported a higher abundance of <em>Thiobacillus</em>, <em>Rhodanobacter</em>, <em>Ferruginibacter</em>, and <em>Sulfurimonas</em> than PRFM. Following wastewater treatment, sulfur content decreased by 77.19% in GCS-PRFM compared with only 6.23% in PRFM, indicating greater sulfur utilization by autotrophic microorganisms. These findings demonstrate that the higher surface area, pore volume, and sulfur availability of GCS-PRFM promote microbial growth and enhance nitrate removal efficiency. The study provides a novel approach for preparing sulfur autotrophic denitrification filters using pyrite and industrial solid waste.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106312"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome and microbial diversity analyses reveal mechanism of GY8 for enhancing cadmium removal rate of duckweed","authors":"Mi Zhang ,&nbsp;Yu-Ting Liu ,&nbsp;Xiang-Qin Li ,&nbsp;Zhi-Qun Chen ,&nbsp;Yan Lan ,&nbsp;Ai-Juan Tan ,&nbsp;Gui-Li Yang","doi":"10.1016/j.ibiod.2026.106313","DOIUrl":"10.1016/j.ibiod.2026.106313","url":null,"abstract":"<div><div>The enhancement of heavy metal remediation efficiency by endophytes in hyperaccumulators has been widely demonstrated. However, research on the influence of endophytes on cadmium (Cd) remediation efficiency of hyperaccumulating duckweed remains limited. In this study, a Cd-resistant endophyte, <em>Agrobacterium fabrum</em> GY8 (GY8), was identified, which increased Cd removal rate of duckweed to 93%. And, colonization of GY8 enhanced duckweed growth rates (up to 117.78%) and alleviated Cd-induced oxidative damage by activating key antioxidant enzymatic pathways. Further investigation revealed that GY8 facilitated phytoremediation through reducing Cd accumulation within duckweed organelles and transforming bioavailable NaCl-extractable Cd into stable HCl-extractable complexes. Whole-genome sequencing identified genes of GY8 associated with Cd resistance and plant growth promotion, including those involved in glutathione metabolism, sulfur cycling, ABC transporter systems, and phenylpropanoid biosynthesis pathways. Microbial diversity analyses confirmed the successful colonization and predominance of GY8 within duckweed. The results provide innovative insights into the mechanisms of endophytes enhancing the Cd removal rate of duckweed, offering theoretical basis for the development of plant-microbe remediation strategies for heavy metal contamination.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106313"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative proteomics insights into the degradation of ethoxylated surfactants by Pseudomonas nitroreducens TX1","authors":"Tuan Ngoc Nguyen ,&nbsp;Fang-Feng Chiu ,&nbsp;Shir-Ly Huang","doi":"10.1016/j.ibiod.2026.106292","DOIUrl":"10.1016/j.ibiod.2026.106292","url":null,"abstract":"<div><div>The bacterium <em>Pseudomonas nitroreducens</em> TX1 (ATCC PTA-6168) is especially interested due to its capability to efficiently remove octylphenol polyethoxylates (OPEO_n), which belong to non-ionic surfactants and commonly used in industrial, agricultural and domestic applications. Although the biodegradation pathways of OPEO_n were studied, the variations at the level of expression of the key enzymes during catabolism are still not quantitatively understood. In this study, we used comparative proteomics analysis approach to determine differential expression and regulation of key enzymes in strain TX1 during octylphenol polyethoxylates utilization as sole carbon source. 43 protein spots (2D gel) and 25 protein bands (SDS-PAGE followed by activity-guided sub-proteomes) significantly up-regulated in OPEO_n-grown cells were identified, whereas 20 protein spots and 11 protein bands were significantly down-regulated. Based on the proteomic results, OPEO_n and its intermediates were proposed in pathways to take up through membrane transport systems, mainly ABC transporters and outer membrane proteins. Key oxidoreductases such as dihydrolipoamide dehydrogenase and NADPH:quinone reductase may drive oxidative degradation of OPEO_n. Elevated levels of FAD/FMN-containing dehydrogenases and NAD-dependent aldehyde dehydrogenases indicate that oxidative reactions were involved in ethoxylate side chains and transferring electrons to the respiratory chain. Our proteomic data also revealed increased expression of glycolate dehydrogenase, isocitrate lyase, and malate synthase, supporting our previous finding of the integration of OPEO_n-derived intermediates into the glyoxylate cycle. Thus, this study provides the first quantitative proteomic insight into OPEOn catabolism in <em>P. nitroreducens</em> TX1.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106292"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative biocodicology: a novel approach combining DNA analysis with FTIR and MicroHot table analysis for the characterisation of modern and ancient parchments","authors":"Ylenia Vassallo ,&nbsp;Elias Lehner ,&nbsp;Monika Waldherr ,&nbsp;Alexandra Graf ,&nbsp;Iulia Maria Caniola ,&nbsp;Salvatore Caterino ,&nbsp;Federica Cappa ,&nbsp;Andreas Hartl ,&nbsp;Marzia Beccaccioli ,&nbsp;Massimo Reverberi ,&nbsp;Katja Sterflinger ,&nbsp;Guadalupe Piñar","doi":"10.1016/j.ibiod.2026.106310","DOIUrl":"10.1016/j.ibiod.2026.106310","url":null,"abstract":"<div><div>This study shows for the first time the potential of combining the nanopore sequencing technology (MinION, ONT) with Whole Genome Amplification (WGA) to identify, simultaneously, endogenous DNA (of animal origin) and environmental DNA (from microbiome) in parchment. This DNA-based approach was integrated with Micro Hot Table (MHT) and Fourier Transform Infrared (FTIR) analysis to assess the deterioration of the parchment.</div><div>The novel strategy was tested on parchments from different ages: three modern parchments of known animal origin, a 19th-century fragment, and nine 14th-15th-century parchment drawings from the Graphic Collection of the Academy of Fine Arts in Vienna. The study compared micro-invasive (small piece of material) and non-invasive (PVC eraser rubbings) sampling. WGA with Nanopore sequencing successfully identified animal species and microbiome composition from both sampling approaches for the first time. The microbiome was dominated by bacteria, primarily human-associated genera (e.g., <em>Staphylococcus, Streptococcus</em>), but also environmental taxa (<em>Acinetobacter, Chryseobacterium, Kaistella, Clostridium,</em> and <em>Pseudomonas)</em>, all well-known for their degradative capabilities, which is consistent with collagen degradation detected by MHT and FTIR. However, it also included viruses (<em>Gemykrogvirus</em>) and protozoa (<em>Babesia</em>). The animal origin was correctly determined for all modern parchment samples used as positive control, and it could also be inferred for the ancient parchment samples using both sampling approaches. The 19th-century parchment was found to be of caprine origin (<em>Capra hircus</em>), while all the ancient drawings were of bovine origin (<em>Bos taurus</em>). This approach merges molecular analysis and degradation study for comprehensive archival research.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106310"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the deteriogenic vascular flora of castles and towers in Campania, Italy","authors":"Alessia Cozzolino ,&nbsp;Giuliano Bonanomi ,&nbsp;Ivana Vitasović-Kosić ,&nbsp;Giandomenico Amoroso ,&nbsp;Riccardo Motti","doi":"10.1016/j.ibiod.2026.106298","DOIUrl":"10.1016/j.ibiod.2026.106298","url":null,"abstract":"<div><div>Biodeterioration is defined as the alteration of stone monuments, wall paintings, wood, paper, vegetal/animal fibers, and parchment artworks, caused by the combined action of physical and chemical factors produced by living organisms colonizing these substrates. The present study examines the role of vascular plants in the biodeterioration of castles and towers in Campania (southern Italy), analysing their relationships with building materials (substrates), exposure, distance from the sea, and elevation. The impact of plant colonization was assessed using the Hazard Index (HI), revealing substrate as the most influential factor both for species diversity and biodeterioration risk. Phanerophytes, known as the most aggressive biodeteriogens, decreased with increasing elevation, despite the greater presence of surrounding wooded areas. This pattern is likely related to the higher occurrence of limestone structures at inland and higher-altitude sites, which are less susceptible to colonization. Finally, distance from the sea and elevation showed no consistent effects on HI, although intermediate ranges exhibited lower HI values, possibly reflecting better maintenance practices at these sites. These findings underscore the need for site-specific conservation strategies that consider substrate vulnerability, such as the high bioreceptivity of tuff and the selective colonization of compact materials like piperno by <em>Capparis spinosa</em>, as well as the role of maintenance practices. The lower Hazard Index values observed in coastal and low-elevation sites suggest that regular interventions are effective in limiting plant-induced deterioration.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106298"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial metabolic remodeling under the electric field: A CUE-centered mechanism for enhanced petroleum bioremediation","authors":"Ruijuan Fan ,&nbsp;Ke Li ,&nbsp;Yang Lin ,&nbsp;Bingyan Yin ,&nbsp;Xingfu Yan","doi":"10.1016/j.ibiod.2026.106307","DOIUrl":"10.1016/j.ibiod.2026.106307","url":null,"abstract":"<div><div>This study explores how bio-electrokinetic remediation (BIO-EK) enhances pollutant degradation by modulating carbon use efficiency (CUE) and microbial functionality. After 100 d, the BIO-EK group achieved a 55.8 ± 1.8% (n = 3) total petroleum hydrocarbon (TPH) degradation rate, significantly higher than bioremediation (BIO, 39.3 ± 2.0%, n = 3) and electrokinetics (EK, 38.7 ± 1.4%, n = 3), with total carbon (TC) content decreasing from 5.0 ± 0.03% (n = 3) to 3.5 ± 0.03% (n = 3), demonstrating its superior pollutant removal effectiveness. Correspondingly, its microbial CUE increased by 1.2-2.4 times, demonstrating that electrokinetic treatment shifted carbon allocation from respiration to biomass synthesis, which was supported by substantially higher microbial biomass and growth rates. Functionally, electrokinetic treatment enriched the abundance of genes for degrading alkanes, fatty acids, and PAHs, activating core metabolic routes such as β-oxidation and the TCA cycle. This was specifically manifested as a marked increase in the abundance of genes encoding pivotal enzymes and their inferred metabolic potential, particularly those related to medium-chain alkane β-oxidation and aromatic hydrocarbon ring cleavage. Structural equation modeling (SEM) revealed that electrokinetic treatment reversed the metabolic trade-off between CUE and degradation in the BIO group, enabling microbes to allocate carbon to both growth and degradation simultaneously. It also showed that electrokinetics enhanced the statistical associations between soil properties, functional genes, and TPH removal, consistent with the proposed causal model. Thus, BIO-EK establishes a highly efficient degradation pathway through the synergistic regulation of environment, genetics, and microbial metabolism.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106307"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BDE-28 degradation using Perinereis gut bacterium: optimization, pathways, and kinetic study","authors":"Pooja Thathola ,&nbsp;Moumita Bhowmik ,&nbsp;Soumya Haldar","doi":"10.1016/j.ibiod.2026.106316","DOIUrl":"10.1016/j.ibiod.2026.106316","url":null,"abstract":"<div><div>Brominated diphenyl ethers (BDEs) are persistent organic pollutants (POPs) of significant environmental concern due to their toxicity and resistance to degradation. The present study demonstrates the efficient biodegradation of 2,4,4′-tribromodiphenyl ether (BDE-28) through <em>Perinereis</em> sp. associated gut bacterium (PGC-2). Under optimized conditions (pH 7.0, 35 °C, 5% inoculum, 50 mg L⁻¹ BDE-28), complete degradation was achieved within 144 h, following a reductive debromination pathway confirmed by GC-MS analysis. Kinetic modeling revealed concentration-dependent growth behavior best described by Richards and Logistic models at high and low BDE-28 levels, respectively. These findings establish polychaete gut microbiota as a previously unexplored and effective biological resource for the biodegradation and detoxification of brominated flame retardants, advancing sustainable bioremediation strategies.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106316"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic strategies of sulfate-reducing microorganisms under energy-limited conditions in oil reservoirs","authors":"Lu Wang ,&nbsp;Panqing Qi ,&nbsp;Aiping Zheng ,&nbsp;Nan Ji ,&nbsp;Minghui Zhou ,&nbsp;Xinmin Song ,&nbsp;Dong Song ,&nbsp;Siqi Li ,&nbsp;Yong Nie ,&nbsp;Weifeng Lv ,&nbsp;Xiao-Lei Wu","doi":"10.1016/j.ibiod.2026.106314","DOIUrl":"10.1016/j.ibiod.2026.106314","url":null,"abstract":"<div><div>Sulfate-reducing microorganisms (SRMs, including both bacteria and archaea taxa) drive bio-corrosion and bio-souring in oil reservoirs. However, the adaptation strategies of SRMs to energy-limited conditions, induced by nutrient competition and metabolic inhibition, challenge the prolonged effectiveness of traditional control strategies. This study provides a comprehensive genomic synthesis of the metabolic strategies employed by SRMs under such constraints to sustain energy metabolism and intracellular redox balance. A total of 752 metagenome-assembled genomes (MAGs) from eight oil reservoir blocks were reconstructed and 60 SRM genomes were identified. Phylogenetic and functional analyses revealed pronounced metabolic heterogeneity between oxidative and reductive DsrAB lineages. Beyond canonical sulfate reduction, SRMs encode a diverse array of sulfur–sulfur bond–cleaving enzymes and hydrogenases, which contribute to redox balancing and energy conservation under energy-limited conditions. Furthermore, the widespread presence of conductive structures including pili and outer-membrane multiheme cytochromes encoded within uncultured SRMs suggests a significant potential for direct or flavin-mediated interspecies electron transfer. Collectively, these findings propose a mechanistic framework for understanding SRM resilience under the energy-limited conditions. These genomic insights also advance the fundamental basis for developing targeted strategies for bio-corrosion and bio-souring control.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106314"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}