International Biodeterioration & Biodegradation最新文献

{"title":"Screening of organophosphorus pesticide-degrading bacteria and a ratio-optimized consortium for efficient phoxim and malathion removal with pathway inference","authors":"Xinran Tang,&nbsp;Mazna Waheed,&nbsp;Qi Xu,&nbsp;Xingrong Liu,&nbsp;Rongrong Xie,&nbsp;Alei Geng,&nbsp;Daochen Zhu","doi":"10.1016/j.ibiod.2026.106419","DOIUrl":"10.1016/j.ibiod.2026.106419","url":null,"abstract":"<div><div>Organophosphorus (OP) pesticides remain as ecotoxic residues in soil and water bodies, and microbial consortia represent a viable bioremediation strategy. Eight OP-degrading bacterial candidates were identified from agricultural soil and irrigation water in Jiangsu, China. Strain PA1, closely related to <em>Pseudomonas aeruginosa</em> JCM 5962 (99.6% 16S rRNA gene similarity), attained 61.0% abiotic-corrected phoxim removal under the initial monoculture screening condition (100 mg L⁻¹, 72 h). Response surface methodology provided a validated operational range of 37°C, pH 7.4, and 4% inoculum, resulting in an 84.4% removal rate. A consortium of PA1 and <em>Stenotrophomonas pavanii</em> SP1 (3:1, 3% of total inoculum) achieved an 87.4% removal of phoxim in single-substrate assays and retained 87.6% phoxim and 85.8% malathion removal in mixed-substrate tests. Liquid chromatography-mass spectrometry detected phoxim-oxon and O, O-diethyl thiophosphate, indicating concurrent hydrolytic and oxidative pathways at the Enzyme Commission class level. Considering the close relationship between PA1 and <em>P. aeruginosa</em>, practical application should consider immobilized cells, cell-free systems, or transfer of catabolic modules to non-pathogenic hosts. This established consortium and its tested framework offer a replicable, evidence-conservative platform for OP reduction.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"214 ","pages":"Article 106419"},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148635430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Genomic and functional characterization of an Antarctic strain of Rhodosporidiobolus odoratus and its hydrocarbon-degradation potential” [Int. Biodeterior. Biodegrad. 214 (2026) 106379]","authors":"G. Cavallini,&nbsp;M.A. Guerreiro,&nbsp;E. Stukenbrock,&nbsp;F. Canini,&nbsp;P. Buzzini,&nbsp;S. Tosi,&nbsp;B. Turchetti,&nbsp;M. Groenewald,&nbsp;S. Garzoli,&nbsp;L. Zucconi","doi":"10.1016/j.ibiod.2026.106416","DOIUrl":"10.1016/j.ibiod.2026.106416","url":null,"abstract":"","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"214 ","pages":"Article 106416"},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148634575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Petroleum microbiology using tiny water droplets","authors":"Ji-Dong Gu","doi":"10.1016/j.ibiod.2026.106418","DOIUrl":"10.1016/j.ibiod.2026.106418","url":null,"abstract":"","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"214 ","pages":"Article 106418"},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148636154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New development on cultural heritage microbiology in China","authors":"Xiaobo Liu,&nbsp;Fasi Wu,&nbsp;Youzhi Feng,&nbsp;Ji-Dong Gu","doi":"10.1016/j.ibiod.2026.106374","DOIUrl":"10.1016/j.ibiod.2026.106374","url":null,"abstract":"<div><div>Culture heritage microbiology is an emerging inter-discipline of cultural heritage and microbiology, which is increasingly developing to meet the necessity of sustainable conservation of world cultural heritage, especially in China. Here, we report a scientific session featuring a special topic of Microorganisms and Cultural Heritage Conservation, which was convened and established for the first at the 14th Annual Geomicrobiology Conference of China in Lanzhou of China on April 17-19, 2026. During this session, 26 presentations on the latest research advances and findings of culture heritage microbiology were delivered by scholars from across the country, followed by active academic discussions and exchanges. The successful convening of this session attracted enthusiastic participation and activated multidiscipline thought exchanges, including microbiology, cultural heritage conservation, environmental science, materials science, engineering, and geology, which will largely promote the innovative development of advanced technologies in the interdisciplinary field of geomicrobiology and cultural heritage conservation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"214 ","pages":"Article 106374"},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148636153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing biological pretreatment and bioleaching processes in sewage sludge: Heavy metal removal mechanisms and EPS-dependent dewaterability enhancement","authors":"Xiaochun Wang ,&nbsp;Jun Li ,&nbsp;Zemiao Shi ,&nbsp;Xiangtong Zhou ,&nbsp;Shanwei Li ,&nbsp;Xiaolei Zhang","doi":"10.1016/j.ibiod.2026.106324","DOIUrl":"10.1016/j.ibiod.2026.106324","url":null,"abstract":"<div><div>To overcome the inhibitory effect of organic matter on sludge bioleaching, this study systematically evaluated the impacts of two pretreatment methods (aeration and anaerobic digestion) on subsequent bioleaching performance. Results demonstrated that aeration pretreatment significantly accelerated the acidification process, enhanced overall heavy metals (HMs) removal efficiency, and effectively minimized phosphorus loss. Following the selection of aeration pretreatment, three bioleaching processes (direct, aerated, and anaerobic bioleaching) were compared. Aerated bioleaching achieved the fastest acidification (pH 2.08 in 6 days) and optimal removal of Cu²⁺, Zn²⁺, and Pb²⁺. Microbial community analysis revealed that the bioleaching process greatly changed community abundance and diversity, while particularly enriching key bioleaching genera such as <em>Acinethiobacillus</em> and <em>Alicyclobacillus</em>. This evolution was closely associated with the selectivity of HMs removal. All bioleaching processes achieved effective sludge reduction (MLSS decreased by 26.99% - 63.21%) and improved dewaterability. Aerated bioleaching yielded the optimal dewatering performance (specific resistance to filtration, SRF: 1.24 × 10¹³ m/kg), attributed to its induced reorganization of extracellular polymeric substances (EPS)—characterized by protein enrichment in tightly bound EPS and polysaccharide stripping from loosely bound EPS. This study provides critical theoretical insights for optimizing sludge bioleaching parameters and pretreatment-process combinations to maximize HM removal while minimizing nutrient loss.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"211 ","pages":"Article 106324"},"PeriodicalIF":4.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial colonization on biocide-treated and untreated façades: The impact of surface orientation","authors":"Michał Ciok ,&nbsp;Daniela Weisenberger ,&nbsp;Moritz Nichterlein ,&nbsp;Stefan Kalkhof ,&nbsp;Matthias Noll","doi":"10.1016/j.ibiod.2026.106311","DOIUrl":"10.1016/j.ibiod.2026.106311","url":null,"abstract":"<div><div>Biocides such as Octylisothiazolinone (OIT) and Terbutryn (TER) are commonly used in building materials to prevent microbial infestation. Their release and antimicrobial efficiency are influenced by wind-driven rain (WDR), which is directly related to façade orientation. This study investigates the bacterial and fungal community composition on biocide-free (BFF) and biocide-containing (BCF) façades with N/W (WDR) or S/E orientation, representing contrasting microclimatic conditions in terms of solar radiation, drying rates, and moisture availability. Sampling was carreid out after one year of outdoor exposure using a cultivation-independent approach. Bacterial and fungal community composition significantly differed between BCF and BFF. Furthermore, the fungal and bacterial community composition was significantly affected by façade orientation. Façades with BCFs S/E showed significantly higher bacterial richness than other façades. Moreover, members of the genera <em>Nesterenkonia, Paracoccus, Microbacteriaceae, Pseudomonas,</em> and <em>Dioszegia</em> dominated BFFs N/W and BFFs S/E, whereas <em>Klenkia</em>, <em>Massilia</em>, <em>Capnodiales</em>, and <em>Filobasidium</em>, were mostly present on BCFs N/W. Only the fungal genus <em>Vishniacozyma</em> was present in every treatment. Bacterial and fungal genera affiliated to biodeterioration, discoloration, and biodegradation, such as <em>Nocardioides</em>, <em>Sphingomonas,</em> Kineosporiaceae, and <em>Arthrobacter</em> were mainly found on BCFs N/W, <em>Arthrobacter</em> was additionally observed on BFFs N/W, and fungal genera <em>Cladosporium</em> and <em>Alternaria</em> were frequently present on BFFs S/E and BFFs N/W. These results demonstrate selective pressure on the microbial colonization patterns by both the presence of biocides and façade orientation. Therefore, these findings provide a broader perspective on the façade microbiome and suggest the need to adjust biocide usage to redefine methods, including considerations of weathering side and biocide application.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106311"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autotrophic denitrification filter prepared using gasification coarse slag and pyrite: Physicochemical characteristics and performance removal of nitrogen","authors":"Shuhan Yang ,&nbsp;Defu Xu ,&nbsp;Jing Su ,&nbsp;Zhengxuan Li ,&nbsp;Alan Howard","doi":"10.1016/j.ibiod.2026.106312","DOIUrl":"10.1016/j.ibiod.2026.106312","url":null,"abstract":"<div><div>Conventional heterotrophic denitrification processes face challenges in achieving the requirements of sustainable development goals due to their dependence on external organic carbon sources and the risk of secondary pollution. In this study, the nitrogen removal performance of a sulfur autotrophic denitrification filter prepared from gasification coarse slag (GCS) and pyrite was evaluated, and its underlying mechanisms were elucidated using microbial community, EDS-SEM, BET, and FT-IR analyses. Pyrite and GCS were combined at a 4:6 mass ratio, followed by the addition of Silica fume (7%), NaOH (4%), and Portland cement (10%) all expressed as percentages relative to the total mass of GCS and pyrite mass). The resulting mixture was granulated to form 10-mm GCS-PRFM particles and steam-cured at 80 °C. The GCS-PRFM exhibited a compressive strength of 3.0 MPa, bulk density of 1272 kg/m³, water absorption rate of 13.5%, specific surface area of 17.00 m²/g, and total pore volume of 0.048 cm³/g. Its specific surface area and total pore volume were 10.3-fold and 16-fold greater, respectively, than those of the pyrite filter (PRFM). The nitrate removal efficiency of GCS-PRFM (81.23%) was approximately double that of PRFM (40.92%). GCS-PRFM supported a higher abundance of <em>Thiobacillus</em>, <em>Rhodanobacter</em>, <em>Ferruginibacter</em>, and <em>Sulfurimonas</em> than PRFM. Following wastewater treatment, sulfur content decreased by 77.19% in GCS-PRFM compared with only 6.23% in PRFM, indicating greater sulfur utilization by autotrophic microorganisms. These findings demonstrate that the higher surface area, pore volume, and sulfur availability of GCS-PRFM promote microbial growth and enhance nitrate removal efficiency. The study provides a novel approach for preparing sulfur autotrophic denitrification filters using pyrite and industrial solid waste.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106312"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome and microbial diversity analyses reveal mechanism of GY8 for enhancing cadmium removal rate of duckweed","authors":"Mi Zhang ,&nbsp;Yu-Ting Liu ,&nbsp;Xiang-Qin Li ,&nbsp;Zhi-Qun Chen ,&nbsp;Yan Lan ,&nbsp;Ai-Juan Tan ,&nbsp;Gui-Li Yang","doi":"10.1016/j.ibiod.2026.106313","DOIUrl":"10.1016/j.ibiod.2026.106313","url":null,"abstract":"<div><div>The enhancement of heavy metal remediation efficiency by endophytes in hyperaccumulators has been widely demonstrated. However, research on the influence of endophytes on cadmium (Cd) remediation efficiency of hyperaccumulating duckweed remains limited. In this study, a Cd-resistant endophyte, <em>Agrobacterium fabrum</em> GY8 (GY8), was identified, which increased Cd removal rate of duckweed to 93%. And, colonization of GY8 enhanced duckweed growth rates (up to 117.78%) and alleviated Cd-induced oxidative damage by activating key antioxidant enzymatic pathways. Further investigation revealed that GY8 facilitated phytoremediation through reducing Cd accumulation within duckweed organelles and transforming bioavailable NaCl-extractable Cd into stable HCl-extractable complexes. Whole-genome sequencing identified genes of GY8 associated with Cd resistance and plant growth promotion, including those involved in glutathione metabolism, sulfur cycling, ABC transporter systems, and phenylpropanoid biosynthesis pathways. Microbial diversity analyses confirmed the successful colonization and predominance of GY8 within duckweed. The results provide innovative insights into the mechanisms of endophytes enhancing the Cd removal rate of duckweed, offering theoretical basis for the development of plant-microbe remediation strategies for heavy metal contamination.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106313"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative proteomics insights into the degradation of ethoxylated surfactants by Pseudomonas nitroreducens TX1","authors":"Tuan Ngoc Nguyen ,&nbsp;Fang-Feng Chiu ,&nbsp;Shir-Ly Huang","doi":"10.1016/j.ibiod.2026.106292","DOIUrl":"10.1016/j.ibiod.2026.106292","url":null,"abstract":"<div><div>The bacterium <em>Pseudomonas nitroreducens</em> TX1 (ATCC PTA-6168) is especially interested due to its capability to efficiently remove octylphenol polyethoxylates (OPEO_n), which belong to non-ionic surfactants and commonly used in industrial, agricultural and domestic applications. Although the biodegradation pathways of OPEO_n were studied, the variations at the level of expression of the key enzymes during catabolism are still not quantitatively understood. In this study, we used comparative proteomics analysis approach to determine differential expression and regulation of key enzymes in strain TX1 during octylphenol polyethoxylates utilization as sole carbon source. 43 protein spots (2D gel) and 25 protein bands (SDS-PAGE followed by activity-guided sub-proteomes) significantly up-regulated in OPEO_n-grown cells were identified, whereas 20 protein spots and 11 protein bands were significantly down-regulated. Based on the proteomic results, OPEO_n and its intermediates were proposed in pathways to take up through membrane transport systems, mainly ABC transporters and outer membrane proteins. Key oxidoreductases such as dihydrolipoamide dehydrogenase and NADPH:quinone reductase may drive oxidative degradation of OPEO_n. Elevated levels of FAD/FMN-containing dehydrogenases and NAD-dependent aldehyde dehydrogenases indicate that oxidative reactions were involved in ethoxylate side chains and transferring electrons to the respiratory chain. Our proteomic data also revealed increased expression of glycolate dehydrogenase, isocitrate lyase, and malate synthase, supporting our previous finding of the integration of OPEO_n-derived intermediates into the glyoxylate cycle. Thus, this study provides the first quantitative proteomic insight into OPEOn catabolism in <em>P. nitroreducens</em> TX1.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106292"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative biocodicology: a novel approach combining DNA analysis with FTIR and MicroHot table analysis for the characterisation of modern and ancient parchments","authors":"Ylenia Vassallo ,&nbsp;Elias Lehner ,&nbsp;Monika Waldherr ,&nbsp;Alexandra Graf ,&nbsp;Iulia Maria Caniola ,&nbsp;Salvatore Caterino ,&nbsp;Federica Cappa ,&nbsp;Andreas Hartl ,&nbsp;Marzia Beccaccioli ,&nbsp;Massimo Reverberi ,&nbsp;Katja Sterflinger ,&nbsp;Guadalupe Piñar","doi":"10.1016/j.ibiod.2026.106310","DOIUrl":"10.1016/j.ibiod.2026.106310","url":null,"abstract":"<div><div>This study shows for the first time the potential of combining the nanopore sequencing technology (MinION, ONT) with Whole Genome Amplification (WGA) to identify, simultaneously, endogenous DNA (of animal origin) and environmental DNA (from microbiome) in parchment. This DNA-based approach was integrated with Micro Hot Table (MHT) and Fourier Transform Infrared (FTIR) analysis to assess the deterioration of the parchment.</div><div>The novel strategy was tested on parchments from different ages: three modern parchments of known animal origin, a 19th-century fragment, and nine 14th-15th-century parchment drawings from the Graphic Collection of the Academy of Fine Arts in Vienna. The study compared micro-invasive (small piece of material) and non-invasive (PVC eraser rubbings) sampling. WGA with Nanopore sequencing successfully identified animal species and microbiome composition from both sampling approaches for the first time. The microbiome was dominated by bacteria, primarily human-associated genera (e.g., <em>Staphylococcus, Streptococcus</em>), but also environmental taxa (<em>Acinetobacter, Chryseobacterium, Kaistella, Clostridium,</em> and <em>Pseudomonas)</em>, all well-known for their degradative capabilities, which is consistent with collagen degradation detected by MHT and FTIR. However, it also included viruses (<em>Gemykrogvirus</em>) and protozoa (<em>Babesia</em>). The animal origin was correctly determined for all modern parchment samples used as positive control, and it could also be inferred for the ancient parchment samples using both sampling approaches. The 19th-century parchment was found to be of caprine origin (<em>Capra hircus</em>), while all the ancient drawings were of bovine origin (<em>Bos taurus</em>). This approach merges molecular analysis and degradation study for comprehensive archival research.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"210 ","pages":"Article 106310"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}