International Biodeterioration & Biodegradation最新文献

{"title":"Dynamic characteristics of attachment genes and their role in aerobic granular sludge development","authors":"Jia Liu ,&nbsp;Xuan Hu ,&nbsp;Yi Yun Gao ,&nbsp;Jianrong Zhu","doi":"10.1016/j.ibiod.2025.106160","DOIUrl":"10.1016/j.ibiod.2025.106160","url":null,"abstract":"<div><div>Aerobic granular sludge (AGS) is a microbial aggregate with a biofilm structure. Understanding microbial attachment at the genetic level is crucial for elucidating the potential mechanisms underlying AGS biofilm formation. This study applied RT‒qPCR to assess the correlation between key attachment genes (<em>rmlA</em>, <em>rpfF</em>, and <em>fliD</em>) and granulation. Comparative analysis of different sludge structures revealed that the abundances of attachment genes were 1–2 orders of magnitude greater in mature AGS than in floccular sludge. This indicated that the degree of microbial aggregation increased in parallel with the abundance of these attachment genes. Furthermore, the presence and dynamic characteristics of three key attachment genes showed significant positive correlations with granulation, particularly granule size (<em>rmlA</em>: r = 0.91, p &lt; 0.001; <em>rpfF</em>: r = 0.88, p &lt; 0.01, <em>fliD</em>: r = 0.97, p &lt; 0.001). Dynamic quantification of attachment gene expression during AGS cultivation revealed stage-specific dominance: <em>rpfF</em> facilitated early-stage aggregation via quorum sensing, whereas <em>fliD</em> exhibited a steep increase (from 1.6 × 10³ to 2.56 × 10⁵ copies·(g·SS)⁻¹) during AGS stabilization, surpassing <em>rmlA</em> (1.47 × 10⁴ copies·(g·SS)⁻¹ and <em>rpfF</em> (1.12 × 10⁵ copies·(g·SS)⁻¹) during maturation. Bioaugmentation with <em>Stenotrophomonas AGS-1</em> increased attachment gene amplification by 2–3 log units while competitively excluding low-abundance microbial groups and selectively enriching functionally dominant consortia. High-attachment bacteria may mediate AGS formation via attachment gene expression. This study revealed aerobic granulation mechanisms as a gene-driven bacterial aggregation process for the first time, highlighting the role of attachment genes in AGS development and providing guidance on biofilm regulation in AGS technology.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106160"},"PeriodicalIF":4.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substrate dynamics on anammox inhibition during domestic wastewater treatment under controlled and ambient temperature","authors":"Divyesh Parde,&nbsp;Ankit Singh,&nbsp;Manaswini Behera,&nbsp;Rajesh Roshan Dash","doi":"10.1016/j.ibiod.2025.106158","DOIUrl":"10.1016/j.ibiod.2025.106158","url":null,"abstract":"<div><div>This study investigates the kinetic behavior and operational performance of an Anammox-based Moving Bed Biofilm Reactor (MBBR) treating synthetic and real domestic wastewater under varying COD/N ratios and hydraulic retention times (HRTs). The objective was to evaluate substrate removal kinetics for total nitrogen (TN) and chemical oxygen demand (COD), assess model applicability, assess and quantify the rates of reactions and identify constraints affecting anammox activity during real domestic wastewater treatment. Three kinetic models: first-order, Grau's second-order, and modified Stover–Kincannon were applied to quantify COD and TN removal. Results showed that while the first-order model provided limited accuracy (R² = 0.36 for COD), both the Grau's model (R² &gt; 0.98) and Stover–Kincannon model (R² &gt; 0.98) demonstrated strong predictive performance for both COD and TN removal. The reactor achieved up to 96 % TN removal and COD removal as high as 92 %, at 24 h HRT. However, higher COD/N ratios (&gt;8) substantially suppressed Specific Anammox Activity (SAA), with up to 92 % inhibition at COD/N = 15, especially under ambient conditions. Comparisons of performance with synthetic and real domestic wastewater further revealed the impact of environmental fluctuations and complex organics on system stability, with TN removal declining from 89 % to 82 % from controlled to ambient scenarios. The study underscores the importance of COD/N ratio management, operational flexibility, and model-guided reactor design in sustaining anammox performance. These findings offer new insights into optimizing Anammox-MBBR reactor for synthetic and real domestic wastewater treatment in decentralized wastewater treatment applications.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106158"},"PeriodicalIF":4.1,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulphate reducing bacteria influenced corrosion of a low carbon steel in marine environment: the role of dissolved organic carbon and temperature","authors":"Michael Olatunde ,&nbsp;Alfred R. Akisanya ,&nbsp;Karen P. Scott ,&nbsp;Jennifer C. Martin ,&nbsp;M. Amir Siddiq ,&nbsp;Srinivas Sriramula ,&nbsp;Alethea Madgett","doi":"10.1016/j.ibiod.2025.106156","DOIUrl":"10.1016/j.ibiod.2025.106156","url":null,"abstract":"<div><div>Sulphate Reducing Bacteria (SRB) are significant contributors to corrosion in marine environments. The corrosion induced by SRB can be influenced by the concentration of Dissolved Organic Carbon (DOC) in the media. In this study, we experimentally investigated the synergistic effects of DOC, temperature, and exposure time on the corrosion rate and morphology of a low-carbon steel in seawater containing SRB. The results showed that the corrosion rate was inversely proportional to the DOC concentration up to 25 days, but directly proportional for the subsequent 100 days of exposure, up to 125 days. There was no significant effect of temperatures between 15 and 25 °C on the corrosion rate. Localised corrosion activity of the SRB was found to be influenced by the heterogeneity of porous biofilm-corrosion product matrix on the steel surface.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106156"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A laboratory approach to mitigating ammonia in broiler litter material with chemical additives","authors":"Busra Yayli,&nbsp;Ilker Kilic","doi":"10.1016/j.ibiod.2025.106157","DOIUrl":"10.1016/j.ibiod.2025.106157","url":null,"abstract":"<div><div>Broiler litter is a major source of ammonia (NH₃) emissions in poultry production systems, negatively impacting both animal welfare and worker health. This study aimed to evaluate the effectiveness of chemical additives in reducing NH₃ volatilization and to identify the optimal application dosage for practical use. The research was conducted in two phases. In Phase I, four additives — aluminum sulfate (Al₂(SO₄)₃), clinoptilolite zeolite, sodium bisulfate (NaHSO₄), and magnesium sulfate (MgSO₄) — were applied to spent broiler litter. Among them, NaHSO₄ demonstrated the highest NH₃ reduction efficiency (76.7 %). In Phase II, varying dosages of sodium bisulfate (2.5 %, 5 %, 7.5 %, and 10 % w/w) were tested, with the 7.5 % application achieving the highest mitigation performance (95.0 %). Additionally, sodium bisulfate treatment improved the ammonium nitrogen content of the litter, enhancing its agronomic value as a potential organic fertilizer. The findings highlight sodium bisulfate as a promising strategy for reducing NH₃ emissions, offering both environmental and economic benefits. This approach may enhance air quality in poultry houses. It can also increase the nutrient value of litter, contributing to sustainable crop production. The laboratory-scale results offer a strong basis for future field studies. These studies are needed to validate the method under commercial production conditions.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106157"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal and pH stress regulate selenite reduction in anaerobic membrane bioreactor: Mechanistic insights into microbial interactions","authors":"Yuanyuan Zhang ,&nbsp;Shuang Liu ,&nbsp;Xiu Shi ,&nbsp;Gaorong Zhang ,&nbsp;Qiaoyan Wei ,&nbsp;Chancui Wu ,&nbsp;Xuehong Zhang ,&nbsp;Junjian Zheng","doi":"10.1016/j.ibiod.2025.106155","DOIUrl":"10.1016/j.ibiod.2025.106155","url":null,"abstract":"<div><div>The anaerobic membrane bioreactor (AnMBR) has emerged as a promising technology for the microbial reduction of selenium (Se)-laden wastewaters. Nonetheless, it remains understudied how the removal performance and mechanism of the AnMBR for Se oxyanions respond to the joint impacts of coexisting pollutants and influent pH. In this study, a lab-scale AnMBR was employed to treat synthetic SeO₃²⁻ wastewater amended with competing electron acceptors (<em>i.e</em>., SO₄²⁻) and coexisting metal ions (<em>i.e</em>., Cd²⁺ and Ni²⁺) at different influent pH. Nearly complete SeO₃²⁻ removal could be attained at moderate pH (5.0−7.0), benefiting from the stable metabolic activity of functional microbial communities. In contrast, a low pH of 3.0 markedly decreased removal efficiencies for all contaminants, although it mitigated membrane fouling to a certain extent. The microbial reduction kinetic rates of SeO₃²⁻ and SO₄²⁻ oxyanions decreased by one order of magnitude as pH declined from 7.0 to 3.0, indicating the strong pH sensitivity of microbial respiration. At moderate pH, metal ions were detoxified primarily through precipitation as metal hydroxides, selenides and sulfides, and partially via complexation by functional groups of microbial products (including proteins, polysaccharides and humic acid). Seven representative functional genera were identified by metagenomic analysis based on known functional roles, including selenium-reducing bacteria (SeRB) and sulfate-reducing bacteria (SRB) with relative abundances greater than 1 %. The dominant and minor SeRB drove the reduction of SeO₃²⁻ toward Se⁰ and Se(−II), respectively, while SRB contributed through enzymatic transformation and abiotic reaction involving biogenic S(−II). At pH 3.0, SRB became nearly undetectable, whereas SeRB persisted with reduced activity, suggesting differential acid sensitivity between the two groups. The findings provided insights into the microbial interaction mechanism of AnMBR for removing multiple oxyanions under metal and proton stress.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106155"},"PeriodicalIF":4.1,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CO2 metabolism and energy acquisition mechanisms of Rhodococcus ruber lz1 under autotrophic conditions","authors":"Qiqi Zhao,&nbsp;Guobao Zhang,&nbsp;Huijie Liu,&nbsp;Xinyu Gao,&nbsp;Lingyi Hou,&nbsp;Qiang Li","doi":"10.1016/j.ibiod.2025.106151","DOIUrl":"10.1016/j.ibiod.2025.106151","url":null,"abstract":"<div><div>In this study, a strain capable of autotrophic growth, <em>Rhodococcus ruber</em> lz1, was isolated from the activated sludge of propylene oxide saponification wastewater. Genomic and transcriptomic analyses revealed the absence of canonical carbon fixation pathways, but identified a complete [NiFe]-hydrogenase maturation system. Notably, hydrogenase subunits (<em>hyaA</em> and <em>hyaB</em>) were significantly upregulated under autotrophic conditions, suggesting the use of trace atmospheric H₂ as an electron donor. The strain is proposed to fix CO₂ via a non-canonical C₁ assimilation pathway proceeding through CO₂ → formate → formaldehyde → CH₂-THF → serine → pyruvate. Genes involved in gluconeogenesis, the TCA cycle, and pyruvate/propionate metabolism were also upregulated, indicating a coordinated metabolic network supporting carbon flux and energy homeostasis. These findings offer new insights into alternative CO₂ fixation strategies in non-classical autotrophic bacteria.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106151"},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Black stains of Lascaux Cave involve different melanin synthesis pathways as shown by Alkaline/H2O2 hydrolysis and UHPLC-ESI/QTOF analysis of pigmented fungal isolates and stains from cave walls","authors":"Pierre Vergne,&nbsp;Malik Bahsoun,&nbsp;Marie-Geneviève Dijoux-Franca,&nbsp;Gilles Comte,&nbsp;Jeanne Doré,&nbsp;Maximilien Gonçalves-Martins,&nbsp;Lise Alonso,&nbsp;Isabelle Kerzaon,&nbsp;Patricia Luis,&nbsp;Yvan Moënne-Loccoz,&nbsp;Serge Michalet","doi":"10.1016/j.ibiod.2025.106142","DOIUrl":"10.1016/j.ibiod.2025.106142","url":null,"abstract":"<div><div>Lascaux cave in southwestern France is a UNESCO landmark famous for its Paleolithic engravings and paintings, but it suffered from microbial outgrowth and black strain formation following unbalanced touristic operations and chemical treatments. Pigments synthesized by black fungi isolated from these stains were previously identified as melanin, but a limited range of black fungi was assessed and black stains themselves have been investigated by surface-enhanced Raman spectroscopy, only. This study aimed at identifying the origins and chemical features of the melanin polymers produced by 10 black fungal strains isolated from Lascaux black stains. Fungi were identified by ITS2 sequencing. Mycelial pigment extracts were analyzed by UHPLC/ESI-QTOF after alkaline H₂O₂ hydrolysis, which allowed to detect simultaneously monomers of different melanin types. Analytical markers from several biosynthetic pathways of melanin were evidenced in all fungal isolates, and some strains showed the ability to produce melanin from at least five different pathways, which to our knowledge constitutes the first report of such a variety of means to achieve black pigmentation within a single fungal strain. As proof of principle, this method was applied to black stains directly collected from the cave and revealed three to four melanin types in samples from the Apse, the Nave and the Passage. Some of these melanin types corresponded to those of the fungal isolates analyzed, namely <em>Alternaria alternata</em>, <em>Acremonium nepalense</em> or <em>Ochroconis lascauxensis</em>. This is the first characterization of the types of melanin synthesized on Lascaux surfaces. Results emphasize the interest of this method to understand different pigmentation patterns in caves and to analyze melanin pigments from diverse biological matrices.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106142"},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremediation of petroleum refinery wastewater using constructed wetlands: A focus on microbial diversity and pollutant degradation","authors":"E.K. Akhiladas,&nbsp;Saswati Chakraborty","doi":"10.1016/j.ibiod.2025.106152","DOIUrl":"10.1016/j.ibiod.2025.106152","url":null,"abstract":"<div><div>Horizontal flow constructed wetlands were evaluated for the treatment of synthetic petroleum refinery wastewater (PRW), with and without <em>Typha latifolia</em>. The synthetic PRW was formulated with a chemical oxygen demand (COD) of 1528.43 ± 125.8 mg/L, and contained diesel, phenol, 49.94 ± 1.48 mg/L of NH₄⁺-N, 142.86 ± 4.26 mg/L of S²⁻, and 265.75 ± 4.28 mg/L of SO₄²⁻. The constructed wetlands (CW) were acclimated for 50 days under continuous flow, followed by three operational phases with varied hydraulic retention times. The synthetic PRW simulated with chemical oxygen demand (COD) of 1528.43 ± 125.8 mg/L, containing diesel and phenol, 49.94 ± 1.48 mg/L of NH₄⁺-N, 142.86 ± 4.26 mg/L of S²⁻, and 265.75 ± 4.28 mg/L of SO₄²⁻. The CW was acclimated for 50 days with continuous flow, followed by three different operational phases with varied hydraulic retention times. Effluent COD in unplanted and planted CW systems were 74.44 ± 16.05 mg/L and 56.67 ± 0.14 mg/L, respectively, with NH₄⁺-N removal rates of 75 % and 92 % over 7 days. S²⁻ removal resulted in solid sulphur deposition and increased the SO₄²⁻ concentration. Microbial dynamics revealed that <em>Proteobacteria</em>, <em>Bacteroidetes</em>, and <em>Firmicutes</em> were the dominant phyla, with genera such as <em>Pseudomonas</em>, <em>Acinetobacter</em>, and <em>Bacillus</em> identified as the key hydrocarbon-degrading bacteria. Gas chromatography-mass spectrometry (GC-MS) analysis confirmed the removal of hydrocarbons, with a reduction in the number of hydrocarbon compounds and generation of intermediate compounds in the effluent. This study highlights the potential application of HFCWs for the treatment of PRW by leveraging the synergistic effects of microbial activity and plant-mediated processes to enhance pollutant removal efficiency.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106152"},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted construction of artificial multicellular SND microbial communities and high ammonia-nitrogen wastewater treatment efficiency analysis","authors":"Xia Ke ,&nbsp;Zu-Lian Liao ,&nbsp;Huan Yu ,&nbsp;Yi-Cheng Zhang ,&nbsp;Shi-Peng Zhou ,&nbsp;Ya-Ping Xue ,&nbsp;Yu-Guo Zheng","doi":"10.1016/j.ibiod.2025.106153","DOIUrl":"10.1016/j.ibiod.2025.106153","url":null,"abstract":"<div><div>Simultaneous nitrification-denitrification (SND) offers a cost-effective approach to nitrogen removal, yet its efficiency is often limited by the instability of natural microbial consortia under fluctuating conditions. This study constructed three artificial multicellular SND microbial communities (consortium A, consortium B, consortium C) by continuously enriching and acclimating using samples from different process sections as the carrier. It elucidated their nitrogen removal mechanisms and engineering application potential. Results demonstrated that consortium B achieved NH₄⁺-N and total nitrogen (TN) removal efficiencies of 95.46 % and 75.84 % within 48 h in a mixed nitrogen source (NH₄⁺-N 350 mg/L, NO₃⁻-N 120 mg/L), with its TN removal rate being 1.10 and 1.16 times higher than those of consortium A and consortium C, respectively. Nitrogen balance and enzyme activity analyses revealed that consortium B exhibited specific activities of 0.6874 U/mg for the key enzyme ammonia monooxygenase (AMO) and 0.4530 U/mg for membrane-bound nitrate reductase (NAR), which were significantly higher than other bacterial flora. The metagenomic analysis revealed that the stable consortium centered on <em>Acinetobacter</em> (62.97 %) was systematically recombined. Among the up-regulated nitrogen metabolism genes, ammonia assimilation, nitrification, and denitrification accounted for 36.85 %, 22.27 %, and 25.03 %, respectively. The nitrogen removal efficiency of consortium B on high-nitrogen wastewater (TN 924.77 mg/L) was 74 % higher than that of the control group in a laboratory-scale bioreactor. This study provides theoretical support and a technological paradigm for optimizing wastewater nitrogen removal using engineered microbial communities.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106153"},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial bioconsolidation of deteriorated limestone exposed to tropical conditions","authors":"Benjamín Otto Ortega-Morales ,&nbsp;Juan Carlos Camacho-Chab ,&nbsp;Manuel Eduardo Ortega-Cano ,&nbsp;Pedro Alberto Camacho-Chab ,&nbsp;Juan Enrique Pereañez-Sacarías ,&nbsp;Joanna María Ortiz-Alcántara ,&nbsp;María Leticia Arena-Ortíz ,&nbsp;Jorge Montero-Muñoz ,&nbsp;María Manuela Reyes-Estebanez ,&nbsp;Carlos Juan Alvarado-López ,&nbsp;Pascual Bartolo-Pérez","doi":"10.1016/j.ibiod.2025.106141","DOIUrl":"10.1016/j.ibiod.2025.106141","url":null,"abstract":"<div><div>Biologically-based treatments are increasingly attracting the interest of the restoration community in Mexico. A microbially induced precipitation (MICP) strategy was implemented as a proof-of-concept based on locally isolated epilithic bacteria that were screened for biocarbonatogenic activity <em>in vitro.</em> Six percent (12) of the 203 tested isolates displayed urease activity and produced crystals with two different media. Isolates TM1B-407, TM1B-475, TM1B-494 and TM1B-508 were the most active isolates on both tests. The most efficient isolate was identified by 16S rRNA gene sequencing as a close relative of <em>Rothia halotoleran</em>s strain YIM 90716. Scanning Electron Microscopy (SEM) showed that <em>Rothia halotolerans</em> TM1B-475 produced crystals, which by μ-X-ray fluorescence (XRF) analysis indicated a calcium carbonate composition; X-ray Photoelectron Spectroscopy (XPS) analysis confirmed that aragonite was the dominant polymorph. Efficiency of the consolidation treatments by <em>Rothia halotolerans</em> TM1B-475, M-3P medium (designed to stimulate the endogenous carbonatogenic community), and Ca(OH)₂ was measured by cohesion (Peeling test) and surface hardness (Equotip) on damaged limestone exposed to tropical conditions and statistically analyzed after 15, 30, 45, 60, and 120 days. There was a clear trend of estimated mean detachment (95 % confidence intervals) for all treatments. On the other hand, there was not a clear trend regarding surface hardness among treatments, but a better performance of the bacterial treatment at the end of the experiment, with an increased hardness of the limestone surface was observed. This study provides the first evidence that the MICP can be used for consolidation purposes in the Yucatan Peninsula on degraded limestone under tropical conditions.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106141"},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}