International Biodeterioration & Biodegradation最新文献

{"title":"Comparative studies on the effectiveness of proteases and enzymes from dietary supplements in the degradation of polylactide (PLA)","authors":"Katarzyna Janczak ,&nbsp;Alicja Mazuryk ,&nbsp;Daria Lisewska ,&nbsp;Natalia Puszczykowska ,&nbsp;Kacper Fiedurek","doi":"10.1016/j.ibiod.2024.105950","DOIUrl":"10.1016/j.ibiod.2024.105950","url":null,"abstract":"<div><div>Polylactide (PLA) has become one of the most widely used biodegradable plastics in recent years. The research focuses on solving the problem of its low-rate biodegradability under mesophilic conditions using cost-effective proteases of alternative origin. The aim of the study was to verify whether enzymes contained in food dietary supplements with the suggested high activity of protease enzymes could potentially be used for the degradation of PLA. The studies compare the activity of enzymes from dietary supplements with hydrolytic enzymes with scientifically proven effectiveness in degrading PLA. Products containing nattokinase, bromelain, papain and an enzyme mixture were tested. Their effectiveness against proteinase K, subtilisin and lipase was compared. From this group, proteinase K caused the greatest PLA mass loss and increase in O/C ratio after 28 days of incubation. The results indicated that nattokinase and papain caused a mass loss of PLA films similar to that of proteinase K, as well as a decrease in the carbonyl index value by more than 20%. Additionally, studies have shown that dietary supplements have a beneficial effect on the compost microflora. Causing an approximately 20-fold increase in the number of microorganisms within 48 h. In accordance with the assumptions, enzymatic products and their concentrations were selected that effectively accelerate PLA degradation in mesophilic conditions at a level comparable to or better than proteinase K, but have a much better effect on the substrate microflora, which makes them an interesting object for the development of products for accelerating the degradation of polymeric materials. In order to confirm the possibility of practical use of the results, an analysis of the phytotoxicity of the enzymes was carried out, which confirmed the possibility of using the enzymes in natural conditions.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105950"},"PeriodicalIF":4.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremediation approaches for chromium detoxification and transformation: Advanced strategies and future Perspectives","authors":"Arukula Deepa ,&nbsp;Anthati Mastan ,&nbsp;Viswanath Buddolla ,&nbsp;Yedluri Anil Kumar ,&nbsp;Buddolla Anantha Lakshmi ,&nbsp;Young-Joon Kim","doi":"10.1016/j.ibiod.2024.105951","DOIUrl":"10.1016/j.ibiod.2024.105951","url":null,"abstract":"<div><div>Hexavalent chromium [Cr(VI)] contamination poses a significant environmental and public health risk due to its widespread industrial use, toxic nature, and carcinogenic properties. Conventional Cr(VI) remediation methods, including electrochemical processes, ion exchange, reverse osmosis, and adsorption, face challenges such as high energy consumption and incomplete detoxification. This review provides a comprehensive evaluation of cutting-edge bioremediation techniques for Cr(VI) removal, with an emphasis on microbial mechanisms involving bacteria and fungi. Bioremediation approaches, particularly those utilizing Cr(VI)-resistant microorganisms, present promising, eco-friendly, and cost-effective solutions for chromium pollution mitigation. The mechanisms of biosorption, bioaccumulation, and bio-reduction employed by these microorganisms are thoroughly examined. Furthermore, the review addresses the scalability and practical application of these biotechnologies in wastewater treatment facilities. This analysis highlights the critical need for innovative, sustainable chromium bioremediation strategies and outlines future research directions to advance this vital field.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105951"},"PeriodicalIF":4.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-fermentation of dark septate endophytes and Trichoderma viride can effectively degrade cellulose in different Chinese medicinal herbal residues","authors":"Wanyun Li ,&nbsp;Menghui Wang ,&nbsp;Xuemin Zhang ,&nbsp;Yali Xie ,&nbsp;Wenjing Chen ,&nbsp;Li Yao ,&nbsp;Chao He ,&nbsp;Xueli He","doi":"10.1016/j.ibiod.2024.105949","DOIUrl":"10.1016/j.ibiod.2024.105949","url":null,"abstract":"<div><div>Chinese medicinal herbal residues (CMHRs) are currently a widely overlooked renewable biomass resource, and developing controllable microbial fermentation technology for CMHRs will help achieve the resource utilization of CMHRs. In this study, the activity of various cellulases, reducing sugar content and cellulose degradation rate were combined to evaluate the solid state fermentation (SSF) potential of dark septate endophytes (DSE) and <em>Trichoderma viride</em> in separate fermentation and co-fermentation of different CMHRs (single <em>Astragalus mongolicus</em> residue and compound residues of <em>Panax notoginseng</em> and <em>Salvia miltiorrhiza</em>). The results showed that the cellulase activity of some DSE strains was higher than that of <em>T. viride</em>. <em>T. viride</em> fermentation alone could increase the content of substrate reducing sugars, while DSE fermentation could reduce the content of substrate reducing sugars. DSE strains such as <em>Macrophomina pseudophaseoline</em>, <em>Paraboeremia selaginellae</em>, <em>Paraphoma chlamydocopiosa</em> and <em>Paraphoma radicina</em> had the potential to co-ferment with <em>T. viride</em>. Optimization of the fermentation process by inoculation of <em>T</em>. <em>viride</em> after 3 d or 6 d of DSE effectively increased the cellulase activity of co-fermented CMHRs. After optimization, the activities of filter paper cellulase, carboxymethyl cellulase, and β-glucosidase in different fermentation combinations reached 2.51–5.61, 2.86–4.72 and 12.85–32.35 U/g, respectively. Further, the co-fermentation of DSE and <em>T. viride</em> effectively degraded cellulose in different CMHRs with a degradation rate of 54.43%–67.38%. Scanning electron microscope confirmed that the co-fermentation of DSE and <em>T. viride</em> could increase the external surface area and porosity of CMHRs, and destroy the structure of CMHRs. The results of this study both provided a basis for the microbial degradation of single and compound CMHR, which is of great significance for solving the treatment of waste such as Chinese herbal residue and further resource utilization.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105949"},"PeriodicalIF":4.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Basalt rock weathering by Peribacillus simplex from Wudalianchi volcanos in NE China and implications for Fe and Si biogeochemical cycling","authors":"Shuang Zhang ,&nbsp;Gaofei Ying ,&nbsp;Tao Liu ,&nbsp;Jiani Yang ,&nbsp;Enci Zhu ,&nbsp;Xindi Sun ,&nbsp;Ji-Dong Gu ,&nbsp;Lei Yan","doi":"10.1016/j.ibiod.2024.105948","DOIUrl":"10.1016/j.ibiod.2024.105948","url":null,"abstract":"<div><div>Bacteria have an important role in weathering the different rocks, but little is known about the mechanism of microbial weathering in basalt rocks. In the present study, <em>Peribacillus simplex</em> WS-L19 isolated from Wudalianchi volcanos exhibits the highest Fe and Si rock dissolution, which revealed unique weathering ability. The optimal weathering conditions of LB medium diluted at 30.9%, the rotation speed of 127.2 rpm, and temperature at 36.6 °C, resulted in the Fe and Si releases of 1.70 ± 0.09 μg/mL and 20.11 ± 1.13 μg/mL, respectively. Simultaneously, complex ligands such as organic acids (tartaric acid, formic acid, lactic acid and succinic acid), siderophores, and biofilm were also found to be involved in weathering processes by <em>P. simplex</em> WS-L19. Kinetics study of Fe and Si release indicated that the weathering processes of volcanic rocks mediated by <em>P. simplex</em> WS-19 fits a Hyperbl model. Mineralogical analysis showed a decrease in Fe releasing ratio by 44.65% and an increase in Si releasing ratio by 28.45%, showing that Fe is more accessible than Si. <em>P. simplex</em> WS-L19 had a significant weathering effect on biotite, and Si-O bonding vibration might contribute to weathering. The results suggest that indigenous <em>P. simplex</em> WS-L19 plays a role in weathering Wudalianchi volcanos and provides new insights into the Fe and Si biogeochemical cycling of basalt rocks.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105948"},"PeriodicalIF":4.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative deterioration of polypropylene by redox mediators and yeast expressing a fungal recombinant laccase","authors":"G. Sabellico ,&nbsp;A. Baggetta ,&nbsp;E. Sandrucci ,&nbsp;G. Zanellato ,&nbsp;A. Martinelli ,&nbsp;A. Montanari ,&nbsp;M.M. Bianchi","doi":"10.1016/j.ibiod.2024.105947","DOIUrl":"10.1016/j.ibiod.2024.105947","url":null,"abstract":"<div><div>Biodegradation of polyolefins is a multistep process that might integrate physical, chemical, enzymatic and/or microbial actions. In this work, we report the use of the laccase redox mediators ABTS and DMP (2,2’ azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and 2,6-dimethoxyphenol, respectively), recycled by laccase activity in aerobic conditions, to induce oxidation of polypropylene film (PP): the laccase was produced in the reaction medium by a recombinant <em>Kluyveromyces lactis</em> yeast strain, expressing a fungal heterologous laccase gene. As a result, we evidenced the formation of oxidize groups on PP surface which increased its water wettablity. We also showed a clear modification of the polymer surface with the appearance of humped and exfoliated regions which favored the break of PP film.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105947"},"PeriodicalIF":4.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABTS mediator enhances biotransformation of fluoranthene by Laccases from Trichoderma lixii FLU1 and Talaromyces pinophilus FLU12","authors":"Samson O. Egbewale ,&nbsp;Ajit Kumar ,&nbsp;Tosin A. Olasehinde ,&nbsp;Mduduzi P. Mokoena ,&nbsp;Ademola O. Olaniran","doi":"10.1016/j.ibiod.2024.105946","DOIUrl":"10.1016/j.ibiod.2024.105946","url":null,"abstract":"<div><div>Fluoranthene poses a significant environmental threat due to its persistence and toxicity. The Laccases from <em>Trichoderma lixii</em> FLU1 (<em>Tl</em>FLU1L) and <em>Talaromyces pinophilus</em> FLU12 (<em>Tp</em>FLU12L) are shown to act as biocatalysts for fluoranthene degradation. 3 U/mL of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L reduced residual fluoranthene concentration to 49.5 ± 8.68 and 61.0 ± 5.66 %, while 10U/mL to 19.2 ± 5.95 and 28.7 ± 1.25 %, respectively, in 96 h. Mixing 200 μM ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with 3 U/mL enzyme in the reaction abolished 100 % residual fluoranthene within 48 h. <em>Tl</em>FLU1L and <em>Tp</em>FLU12L exhibited almost similar <em>v</em>_max (1.35 ± 0.02 and 1.29 ± 0.15 mg/L/h, respectively), but <em>Tl</em>FLU1L showed a lower <em>K</em>_m as compared to <em>Tp</em>FLU12L (119.2 ± 0.02 and 170.8 ± 0.15 mg/L, respectively). ABTS significantly increased <em>v</em>_max to 7.73 ± 0.23 and 7.97 ± 0.28 mg/L/h, and decreased <em>K</em>_m to 54.8 ± 0.27 and 26.6 ± 0.21 mg/L for <em>Tl</em>FLU1L and <em>Tp</em>FLU12L, respectively. GC-MS analysis revealed that <em>Tl</em>FLU1L generated metabolites 9-oxo-fluorene-1-carboxylic acid, 9H-fluoren-9-one, and phthalic acid while <em>Tp</em>FLU12L produced 9,10-phenanthrenedione and benzene-1,2,3-tricarboxylic acid. Ecotoxicity and cytotoxicity analysis of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L degradation products in the presence of the mediator (ABTS) are found to be non-toxic towards marine bacteria (<em>Vibrio parahaemolyticus</em>) and HT22 cells. Thus, the study underscores the promising potential of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L, particularly in conjunction with mediator (ABTS), for environment friendly and efficient bioremediation of fluoranthene-contaminated environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105946"},"PeriodicalIF":4.1,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142555051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of sheep myeloid antimicrobial peptide (SMAP-18) and siderophore Pyochelin for mitigation of aerospace fuel-degrading microbes","authors":"Amanda L. Barry Schroeder ,&nbsp;Osman Radwan ,&nbsp;Oscar N. Ruiz ,&nbsp;Thusitha S. Gunasekera ,&nbsp;Andrea Hoffmann","doi":"10.1016/j.ibiod.2024.105943","DOIUrl":"10.1016/j.ibiod.2024.105943","url":null,"abstract":"<div><div>Sustainment of aviation fuel infrastructure is expensive and requires frequent testing for hydrocarbon-degrading microbes to assure safeguarding of fuel quality and engineering systems. Microbial contaminants in Jet fuel, including Gram-positive and Gram-negative bacteria, yeast, and filamentous fungi, necessitate early action to prevent biofouling and biocorrosion. Recent studies have identified two novel antimicrobial agents, the sheep myeloid antimicrobial peptide SMAP-18 and the iron chelating siderophore, Pyochelin with potential suitable antimicrobial properties for jet fuel sustainment. This study evaluates the antimicrobial activity of SMAP-18 and Pyochelin in specialized antimicrobial assays including liquid and fuel-culture minimum inhibitory concentration (MIC) testing, small-scale (25 mL) and large-scale (1 L) Jet A fuel microbial consortium cultures. The results show that repetitive dosing of combined SMAP-18 and Pyochelin is bactericidal and able to control bio-contaminant progression of Gram-positive <em>Gordonia</em> sp., and Gram-negative <em>Pseudomonas putida</em> bacteria in jet fuel. Moreover, the synergy observed between SMAP-18 and Pyochelin highlights their complementary mechanisms of action against microbial targets resulting in complete elimination of bacterial growth with a −7.9 log fold reduction through day 24.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105943"},"PeriodicalIF":4.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Fenton combined with membrane bioreactor in the treatment of printing and dyeing wastewater","authors":"Haibo Xu ,&nbsp;Han Zhang ,&nbsp;Jincan Huang ,&nbsp;Lu Zhang ,&nbsp;Feng Wang ,&nbsp;Guangbing Liu ,&nbsp;Xuemin Yu ,&nbsp;Weijing Liu ,&nbsp;Chunkai Huang","doi":"10.1016/j.ibiod.2024.105945","DOIUrl":"10.1016/j.ibiod.2024.105945","url":null,"abstract":"<div><div>Effects of influent adsorbable organic halogens (AOX) and ammonia concentrations, hydraulic retention time (HRT) on the microbial community composition, functional genes abundance of anoxic/aerobic-membrane bioreactor (A/O–MBR) and its ability to treat raw and Fenton oxidized printing and dyeing wastewater (PDW) were evaluated. Fenton combined with A/O–MBR exhibited better treatment performance on chemical oxygen demand (COD) and AOX removal for PDW remediation. After six months’ optimization, at phase VII (50 mg/L AOX, 50 mg/L NH₄⁺–N, and HRT = 72h), 81.8 % of the COD and 94.6% of the AOX were removed in raw PDW A/O–MBR, and 92.0% of the COD and 100% of the AOX were removed in Fenton oxidized PDW A/O–MBR. An increase in ammonia level is conducive to the COD and AOX removal, whereas shortening the HRT in the A/O–MBR systems can drastically lower the COD and AOX removal efficiency. The metagenomics analysis indicates that an increase in AOX loading facilitates the accumulation of 2,4,6–TCP and 4–CP metabolism relevant genes, while significantly reduces the transcript per million (TPM) abundance of nitrogen removal functional genes. However, higher ammonia loading and shorter HRT promote the accumulation of AOX and nitrogen removal functional genes.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105945"},"PeriodicalIF":4.1,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new enrichment strategy of dissimilatory iron-reducing bacteria for remediation of organic-contaminated river sediments: Process, performance, and mechanism","authors":"Qian Chen ,&nbsp;Bin Wang ,&nbsp;Weihang Liang ,&nbsp;Suyun Chang ,&nbsp;Jianjun Huang ,&nbsp;Jingmei Sun","doi":"10.1016/j.ibiod.2024.105944","DOIUrl":"10.1016/j.ibiod.2024.105944","url":null,"abstract":"<div><div>Dissimilatory iron-reducing bacteria (DIRB) have been widely applied to organic matter metabolism in sediments due to their specific biological properties, which are significantly affected by environmental factors. Until now, there has been no systematic study on applying these effects of environmental factors to DIRB enrichment. In this study, we identified the critical environmental factors through RDA, PLS-PM, and correlation analysis and then adjusted the proportion of these factors (C/Fe, C/N, and Fe/S) through single-factor and response surface experiments to enrich DIRB. The performance of the enriched DIRB in carbon metabolism of organic-rich sediments was analyzed by remediation experiment, and the metabolic mechanism was revealed through iron-reducing performance, community structure, and functional genes. The results showed that the carbon, nitrogen, phosphorus, and sulfur were crucial factors influencing the activity of DIRB in river sediments, and the enriched mud-bacteria mixture containing a large amount of DIRB (referred to as “DMB”) obtained by domestication under C/Fe = 0.492 and C/N = 13.659 showed the highest iron reduction rate of 55.51% ± 2.53%. The DMB exhibited better-sustained removal of total organic matter (TOM), which was higher than the control (MB) by 8.81%. Moreover, we discovered that the enriched DIRB: (1) had a better reduction effect on different Fe(III) forms, especially in carbon-limited and iron-limited conditions. (2) increased in abundance and established a beneficial symbiotic relationship with hydrolytic acidifying bacteria. (3) showed an increased abundance of functional pathways associated with organismal systems and signal processing (such as ABC transporters and Translation). These findings revealed the reasons for the improved efficiency of organic matter metabolism.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105944"},"PeriodicalIF":4.1,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A field-scale assessment of the efficacy of bioremediation of petroleum hydrocarbon-contaminated soils using two biostimulants","authors":"Wen-Hsien Tsai ,&nbsp;Tzu-Wen Chen ,&nbsp;Yuan-Hung Liu ,&nbsp;Shih-Mao Shen ,&nbsp;Colin S. Chen ,&nbsp;Chien-Jung Tien","doi":"10.1016/j.ibiod.2024.105942","DOIUrl":"10.1016/j.ibiod.2024.105942","url":null,"abstract":"<div><div>Nutrients are known limiting factors for the successful bioremediation of soil contaminated with petroleum hydrocarbons (PHCs). Thus, two biostimulants, soil bioremediation nutrients (SBNs) and nutrient solution (NS), were tested to assess their effects on the degradation of PHCs and changes in bacterial community compositions, functions and functional genes using third generation sequencing, Tax4Fun2 and qPCR at the field scale. The addition of SBNs or NS significantly increased and accelerated the degradation of total petroleum hydrocarbons (TPH). The highest TPH degradation rate (96%) and k (0.134/day) were shown for biopiles with SBNs addition (particularly for 0.5% SBNs), indicating that SBNs were a good biostimulant for bioremediation of PHC-contaminated soil in the field. After different operations, rapid reconstruction of bacterial communities and functions for 5 biopiles was found with relatively high metabolic function and a change in dominant PHC degraders from <em>Rugosibacter aromaticivorans</em> and <em>Novosphingobium aromaticivorans</em> to <em>Immundisolibacter cernigliae</em> and <em>Dyella ginsengisoli</em>. The results of Tax4Fun2 prediction revealed that the addition of SBNs and water with soil turning enhanced and accelerated bacterial metabolic functions and related functional genes for degrading aromatic compounds. The qPCR results indicated that most samples contained a relatively high abundance of <em>alkB</em>, <em>alkB1</em>, <em>XylE</em> and <em>PHE</em>, and high expression of <em>XylE</em> and <em>PHE</em>, while the addition of SBNs and NS enhanced the expression of <em>XylE</em> and <em>PHE</em> for degrading catechol and phenol. Concentrations of TPH and nutrients were major environmental factors influencing the degradation of TPH, alkane and aromatic compounds and changes in bacterial communities, functions and functional genes (e.g., <em>alkB</em>, <em>alkB1</em>, <em>XylE</em>, <em>NAH, PHE</em> and <em>BPH4</em>). Biostimulation by the addition of SBNs proved an effective strategy for increasing the degradation of PHCs and facilitating the growth and metabolic activity of PHC degraders.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105942"},"PeriodicalIF":4.1,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}