Christine C. Gaylarde , Beata Gutarowska , Iwona B. Beech , Justyna Szulc , Amit Acharjee , Tomasz Grzyb , Alex da Silva de Freitas , José Antônio Baptista Neto
{"title":"Metagenomic and morphological analysis of biodeteriogenic biofilms on historic stone buildings in the tropical north-east of Brazil","authors":"Christine C. Gaylarde , Beata Gutarowska , Iwona B. Beech , Justyna Szulc , Amit Acharjee , Tomasz Grzyb , Alex da Silva de Freitas , José Antônio Baptista Neto","doi":"10.1016/j.ibiod.2025.106143","DOIUrl":"10.1016/j.ibiod.2025.106143","url":null,"abstract":"<div><div>Samples of dark-colored biofilms collected from the surfaces of three 16th and 18th century buildings in equatorial Brazil, two limestone churches in Salvador, Bahia, and a sandstone fort in Belém, Para, were characterized using metagenomics, light microscopy and field emission scanning electron microscopy (FESEM) with energy-dispersive X-ray spectroscopy (EDS). Cyanobacteria were dominant colonisers, <em>Chroococcidiopsis</em> and <em>Trichotorquatus</em> on Salvador churches, and <em>Aerosakkonema</em> on the Belém fort. Actinomycetes and extremophiles were more common in hotter and more humid Belém than in Salvador. Major fungal sequences represented <em>Sordariomycetes</em> and <em>Dothideomycetes</em>, the latter being more frequent in Belém. The presence of several bacterial species and of the diatom <em>Humidophila contenta</em> are reported for the first time on historic stone buildings. Apart from microbially induced discoloration, biogenic deposits of goethite and possibly pyrite were found within the stone, contributing to its biodeterioration.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106143"},"PeriodicalIF":4.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sulfate reducing activity and corrosion potential of diverse heterotrophic facultative bacteria isolated from crude oil pipeline residues","authors":"C.A. Shefeena , Jesmi Yousuf , C.B. Sudheer , Mohamed Hatha A.A , Anand K.P","doi":"10.1016/j.ibiod.2025.106138","DOIUrl":"10.1016/j.ibiod.2025.106138","url":null,"abstract":"<div><div>Sulfate-reducing bacteria are vital in biogeochemical cycles and can significantly affect both environmental and industrial processes, including biocorrosion. This study investigates the sulfate-reducing potential of various heterotrophic facultative bacteria isolated from crude oil samples collected at various points in a petroleum refinery, after a crude oil pipeline pigging operation. The samples were analysed to identify the microbial communities and their metabolic capabilities. Bacterial strains of <em>Enterobacter cloacae, Enterobacter ludwigii, Enterobacter hormaechei, Citrobacter braakii, Citrobacter freundii</em> and <em>Pantoea hericii</em> were isolated from the crude oil samples using selective enrichment. Their sulfate reducing potential was assessed by measuring sulfide production and growth under various environmental conditions. Significant variability in sulfate-reducing activity among the isolated strains, with some exhibiting notably high reduction rates. Molecular identification via 16S rRNA sequencing was employed to characterize the strains and elucidate their phylogenetic relationships. Furthermore, corrosion capacity of the most promising bacterial strain among those identified, <em>E</em><em>.</em> <em>cloacae</em> on API 5L X65 pipeline material was analysed using the potentiodynamic polarization method. Under abiotic conditions, the corrosion rate was measured at 0.09 mm per year, with a corrosion current density of 1.69 × 10<sup>−5</sup> A cm<sup>−2</sup>. However, in the presence of facultative bacterium <em>E</em><em>.</em> <em>cloacae,</em> the corrosion rate increased significantly to 0.23 mm per year, accompanied by corresponding I<sub>corr</sub> value of 3.29 × 10<sup>−5</sup> A cm<sup>−2</sup>. This study contributes to a better understanding of the ecological roles of facultative sulfate reducing bacteria in industrial environments, especially within crude oil processing.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106138"},"PeriodicalIF":4.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Formulations of chitosan/TiO2/ZnO ternary nanocomposites-based alkyd nanopaint for marine anti-biofouling application","authors":"Lakshminarayanan Sivakumar , Santhosh Kumar Arunagiri , Dhandapani Perumal , Manikandan Thiyagarajan , Prasanth Bhatt N , Suraj R , Suriyaprakash Rajadesingu , Murugesan Perumal , Rajaram Rajamohan , Parthipan Punniyakotti","doi":"10.1016/j.ibiod.2025.106136","DOIUrl":"10.1016/j.ibiod.2025.106136","url":null,"abstract":"<div><div>Marine biofouling is a significant challenge for the performance and durability of marine vessels and structures. This study focuses on developing a chitosan/TiO<sub>2</sub>/ZnO nanocomposite-based alkyd nanocoating to mitigate biofouling. Chitosan was effectively extracted from shrimp shell, pristine ZnO and TiO<sub>2</sub> nanoparticles were synthesized to form binary and ternary nanocomposites. The resultant materials were characterized using various analytical techniques. UV–Visible spectroscopy confirmed that the ternary nanocomposites exhibited a shift in the absorption edge at the interface between pristine TiO<sub>2</sub> and ZnO. X-ray diffraction and High-Resolution Transmission Electron Microscope confirmed wurtzite ZnO as hexagonal nano rods and anatase-phase TiO<sub>2</sub> with a spherical morphology. Raman and X-ray photoelectron spectroscopy confirmed the presence of pristine TiO<sub>2</sub> and ZnO nanoparticles in the composites. The chitosan/TiO<sub>2</sub>/ZnO composite materials were evaluated at a concentration of 50 μg/mL and demonstrated enhanced antibacterial and antibiofilm activities. In addition, alkyd resin-based nanopaints were formulated by incorporating these nanocomposites and coated onto mild steel coupons. The residue weight of TiO<sub>2</sub> and ZnO (32.49 % in 760 °C) in the formulation was confirmed by thermogravimetric analysis. The water contact angle measurement indicated a hydrophobicity of 90.17° for the ternary composite. Mesocosm experiments and field exposure trials showed a notable reduction in microfouling and macrofouling on surfaces coated with chitosan/TiO<sub>2</sub>/ZnO paint. Notably, the barnacle attachment count on coupons submerged in seawater for 45 days showed 10 % adherence on the chitosan/TiO<sub>2</sub>/ZnO paint. The results of the field immersion study indicate that this nanopaint exhibits significant potential as an effective material for marine antifouling applications.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106136"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Susceptibility of fungi to commercially available traditional chemical products and to potentially alternative products for use in cultural heritage preservation","authors":"Johann Leplat , Alexandre François , Faisl Bousta","doi":"10.1016/j.ibiod.2025.106137","DOIUrl":"10.1016/j.ibiod.2025.106137","url":null,"abstract":"<div><div>Traditional chemical biocide products are still widely used to control fungal development in the field of cultural heritage preservation, despite increasing criticism related to environmental and human health concerns. Moreover, the efficacy of biocidal products varies depending on the type of micro-organism targeted. It is therefore crucial to assess the susceptibility of fungal species to biocide compounds prior to their use. This study tests the susceptibility of 193 different fungal species to eight commercially available biocide products. Five of these products were classical biocides that target all microorganism deposits, and were composed of either quaternary ammonium salts or a combination of quaternary ammonium salts and isothiazolinone. One biocide was composed of econazole nitrate and specifically targeted fungi. The two remaining biocides were greener products designed solely for green deposits, and were assessed as potential alternatives to classical biocides. They were composed of lemongrass essential oil and pelargonic acid, respectively. The susceptibility to biocides was genus dependent for several fungal genera, while it was species dependent in other cases. The classical biocide product Biotin T™ was the most efficient in inhibiting fungal growth overall. Only weak fungal growth inhibition was obtained with the two products that were tested as alternative solutions. The fungi-specific product was very efficient in some cases but inefficient in others, thus underlining the importance of testing the products before use. These results showed that however desirable alternative solutions to traditional biocide products may be, there are actually very few commercially available alternative solutions.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106137"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xinyue Zhang , Xuan Qiu , Weiqi Wang , Min Li , Jingwen Lei , Olli H. Tuovinen , Hongmei Wang
{"title":"Microbial reductive dissolution of schwertmannite leading to antimony release","authors":"Xinyue Zhang , Xuan Qiu , Weiqi Wang , Min Li , Jingwen Lei , Olli H. Tuovinen , Hongmei Wang","doi":"10.1016/j.ibiod.2025.106133","DOIUrl":"10.1016/j.ibiod.2025.106133","url":null,"abstract":"<div><div>Schwertmannite, an iron hydroxysulfate mineral, significantly influences the mobility of antimony. While schwertmannite can effectively sequester antimony through adsorption or coprecipitation, the subsequent release of antimony from schwertmannite via microbial activities remains poorly understood. This study revealed that up to 40% of the Sb(V) coprecipitated by schwertmannite can be released by the iron-reducing bacterium <em>Shewanella piezotolerans</em> WP3 through dissimilatory iron reduction, Over 50% of crystalline Fe(III) in schwertmannite was reduced by WP3, facilitating the dissolution of schwertmannite and the release of Sb(V), accompanied by partial Sb(V) reduction to mobile Sb(III) (3.10–3.34% of total Sb). Vivianite emerged as the predominant secondary mineral in the presence of WP3 as confirmed by XRD spectra. In contrast, under the pH around 6.8 (mimicking the circumneutral condition during antimony remediation) in abiotic control, Sb(V)-loaded schwertmannite remanded relatively stable with 2.13–3.04% Sb(V) released. These findings underscore the critical role of microbial Fe(III) reduction in mobilizing antimony from Sb-bearing minerals, thereby highlighting potential contamination risks associated with microbial activity.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106133"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junbin Ji , Jing Pei , Fanghui Ding , Jie Zhou , Weiliang Dong , Zhongli Cui , Xin Yan
{"title":"Biodegradation of polyurethane plastic monomer 4,4′-methylenedianiline by Rhodococcus sp. YXP8","authors":"Junbin Ji , Jing Pei , Fanghui Ding , Jie Zhou , Weiliang Dong , Zhongli Cui , Xin Yan","doi":"10.1016/j.ibiod.2025.106135","DOIUrl":"10.1016/j.ibiod.2025.106135","url":null,"abstract":"<div><div>The 4,4′-methylenedianiline (MDA) is an important precursor in the production of polyurethane plastic. The environmental release of MDA poses a threat to plants, animals, and humans. Microorganisms exert an important role in degrading xenobiotic compounds. However, only several MDA-degrading microbes are reported, and the molecular mechanism of MDA degradation remains unclear. In this work, a Gram-positive MDA-degrading bacterium <em>Rhodococcus</em> sp. YXP8 was first obtained from the surface of polyurethane waste. This strain could degrade 20 mg·L<sup>−1</sup> MDA at 30 °C and pH 7.0 within 4 days and showed good degradation ability (>70 %) within a wide pH range from 5.0 to 10.0. The results of liquid chromatograph-tandem mass spectrometer analysis indicate that strain YXP8 transformed MDA to two final products of 4-aminophenylacetic acid and (Z)-3-amino-2-hydroxypenta-2,4-dienoic acid. The catabolic pathway of MDA in bacteria was demonstrated for the first time. Strain YXP8 could efficiently remediate MDA-contaminated natural water, eliminating its risk to aquatic organisms. Taken together, this work presents a bacterium with potential for biological treatment of MDA pollutants and for mining genetic determinants of MDA degradation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106135"},"PeriodicalIF":4.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Human-derived glutathione S-transferase mu 1 mediated hydrolysis of nitrocellulose: reaction mechanisms and thermal decomposition behavior","authors":"Huihui Xue , Qiaoyu Chen , Juan Huang , Alei Zhang , Yajun Ding , Zhongliang Xiao","doi":"10.1016/j.ibiod.2025.106139","DOIUrl":"10.1016/j.ibiod.2025.106139","url":null,"abstract":"<div><div>This study proposes the innovative application of human-derived glutathione S-transferase mu 1 (GSTM1) for biocatalytic denitration of nitrocellulose (NC), designed to achieve nitrate ester hydrolysis under mild conditions while effectively reducing NC's hazardous properties. The experimental results showed that GSTM1 protein was expressed heterologously in engineered <em>Escherichia coli</em> (<em>E. coli</em>). After 48 h of treatment with purified enzyme GSTM1, the nitrogen content of NC significantly decreased from 12.97 % to 8.4 %. Fourier transform infrared spectroscopic analysis combined with cellulase enzymatic hydrolysis experiments of substances insoluble in ethyl acetate demonstrated GSTM1-mediated conversion of nitrate ester groups to hydroxyl groups in NC, accompanied by hydrogen bond formation and subsequent regeneration of cellulose. Gel permeation chromatography revealed that GSTM1 treatment significantly reduced the relative molecular weight of NC while broadening its molecular weight distribution. Moreover, scanning electron microscopy images revealed increased crack density and surface roughness on NC fiber. The TG-DSC and DSC experiments confirmed decreased combustion heat and reduced activation energy (E<sub>a</sub>) from 195.92 kJ·mol<sup>−1</sup> to 168.47 kJ·mol<sup>−1</sup> post-treatment of NC. The thermal decomposition process of NC before and after the reaction was thoroughly analyzed using the model-free Friedman method. Through systematic optimization, the ideal enzymatic reaction parameters were established as 3.2 g·L<sup>−1</sup> NC substrate concentration with 1.0 g·L<sup>−1</sup> GSTM1 at 37 °C for 8 h. This novel technique safely and environmentally friendly treats waste military compounds while offering new insights for developing multi-enzyme synergistic systems for NC-based waste treatment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106139"},"PeriodicalIF":4.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zizhuo Li , Jinya Wei , Jitao Li , Yaling Yang , Dezhi Yang , Yuzhu Song
{"title":"Ciprofloxacin-based zinc-doped carbon dots with peroxidase-like activity against Escherichia coli and biofilm eradication","authors":"Zizhuo Li , Jinya Wei , Jitao Li , Yaling Yang , Dezhi Yang , Yuzhu Song","doi":"10.1016/j.ibiod.2025.106134","DOIUrl":"10.1016/j.ibiod.2025.106134","url":null,"abstract":"<div><div>Herein, we rationally designed a novel and forceful ciprofloxacin (CIP) grafted zinc-doped-carbon dots (CIP@Zn/CDs) as an efficient artificial nanozyme against <em>Escherichia coli</em> (<em>E. coli</em>), multi-drug-resistant <em>E. coli</em> (AREC), and <em>E. coli</em> biofilms through the peroxidase (POD)-like catalytic activity and the synergistic antibacterial activity of CIP and Zn<sup>2+</sup>. On the one hand, extracellular DNA (eDNA) cleave by high levels of hydroxyl radicals (•OH) catalyzed by the POD-like catalytic activity of CIP@Zn/CDs. On the other hand, CIP@Zn/CDs with positive surface potential, elimination abilities against <em>E. coli</em> via electrostatic interaction, changing the cell membrane permeability and eventually leading to <em>E. coli</em> death within <em>E. coli</em> biofilms. Furthermore, the ultrasmall size of CIP@Zn/CDs possesses good biocompatibility favoring clinical translation. This work provides potential to develop CD-based techniques for applications in the field of selective antibacterial agents towards Gram-negative bacteria.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106134"},"PeriodicalIF":4.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ching Yi Hong , Khalida Muda , Mohamad Ali Fulazzaky
{"title":"Mechanisms and mass transfer kinetics of anammox during sludge enrichment","authors":"Ching Yi Hong , Khalida Muda , Mohamad Ali Fulazzaky","doi":"10.1016/j.ibiod.2025.106140","DOIUrl":"10.1016/j.ibiod.2025.106140","url":null,"abstract":"<div><div>Mass transfer kinetics for enrichment of anammox granular sludge (AnGS) is important for optimizing the removal of ammonium (NH<sub>4</sub><sup>+</sup>) from wastewater. AnGS enrichment was monitored over 147 days in a continuous upflow sludge bed anammox reactor (CUSBAR) experiment, which was simulated using Generalized Fulazzaky equations to predict the underlying mechanisms and mass transfer kinetics. The removal efficiency of NH<sub>4</sub><sup>+</sup> followed the bacterial growth progressed through four distinct phases over 63 days, starting with an initial NH<sub>4</sub><sup>+</sup> concentration of 30.9 mg/L. The CUSBAR process achieved a performance of 84.97 % on the 147th day of the experiment, with the initial NH<sub>4</sub><sup>+</sup> concentration increased from 30.9 to 75.0 mg/L. The internal mass transfer (IMT) and global mass transfer rates were nearly identical and significantly higher than the external mass transfer (EMT) rate, indicating that EMT was the dominant resistance to mass transfer. The positive correlation between NH<sub>4</sub><sup>+</sup> removal efficiency and IMT rate provides valuable insights into the dynamic response of AnGS development during the 147-day enrichment period. This study's findings enhance the availability of AnGS as inocula for future CUSBAR process applications, expanding the knowledge base and references in anammox-based nitrogen removal technology.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106140"},"PeriodicalIF":4.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Loryn L. Bowen , Molly E. Chapleau , Richard R. Chapleau , Oscar N. Ruiz , Thusitha S. Gunasekera
{"title":"Novel bio-recognition elements (BREs) for biosensing and differentiation of Gram-positive bacteria in hydrocarbon environments","authors":"Loryn L. Bowen , Molly E. Chapleau , Richard R. Chapleau , Oscar N. Ruiz , Thusitha S. Gunasekera","doi":"10.1016/j.ibiod.2025.106130","DOIUrl":"10.1016/j.ibiod.2025.106130","url":null,"abstract":"<div><div>Excessive microbial growth in fuel and fuel systems can lead to biofouling and biocorrosion, which makes early detection crucial. A wide range of phylogenetically and metabolically diverse microorganisms have been isolated from environments exposed to hydrocarbons with Gram-positive and Gram-negative bacteria being ubiquitous. The aim of this work was to identify specific biomarker-based bio-recognition elements (BREs) for sensing hydrocarbon-degrading Gram-positive bacteria. We used two bacterial surface targets that are conserved and specific for Gram-positive bacteria, Lipoteichoic acid (LTA) and a short surface-epitope region from the cell wall-associated secreted protein Fasciclin 1 (FASC1), from <em>Gordonia sihwensis</em>. Several peptide-based ligands to detect numerous Gram-positive bacteria in fuel were discovered by using phage display technology and the binding of these peptides to Gram-positive bacteria in the presence of hydrocarbons was validated using Microscale Thermophoresis (MST) and Western blot analysis. The binding peptides were conjugated to quantum dots and their specificity to Gram-positive bacteria was characterized by fluorescence imaging and fluorometric analysis in the presence of target and non-target microorganisms. It was shown that jet fuel did not interfere with binding of the peptides to the target biomarker in the bacteria, indicating that these BREs are suitable for functionalization of biosensors for detection of Gram-positive bacteria in hydrocarbon rich environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106130"},"PeriodicalIF":4.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}