International Biodeterioration & Biodegradation最新文献

{"title":"Metatranscriptomic profiling reveals microbial succession and adaptation strategies during long-term lignocellulose degradation","authors":"Lucas Amoroso Lopes de Carvalho ,&nbsp;Anna Carolina de Oliveira Souza ,&nbsp;Camila Cesário Fernandes ,&nbsp;Cleiton Dias do Prado ,&nbsp;Eliana Gertrudes de Macedo Lemos ,&nbsp;Lúcia Maria Carareto Alves ,&nbsp;Daniel Guariz Pinheiro","doi":"10.1016/j.ibiod.2025.106110","DOIUrl":"10.1016/j.ibiod.2025.106110","url":null,"abstract":"<div><div>Lignocellulosic biomass is an abundant and renewable feedstock, promising for biofuel production and other industrial applications; however, it presents numerous challenges due to its complex structure. Alternatively, microbial systems naturally possess robust mechanisms capable of efficiently degrading this material. In this study, we investigated the functional and compositional changes of a microbial community in the early and late stages of adaptation to a lignocellulosic substrate using a metatranscriptomic approach. The results revealed a transition in the contribution of major phyla associated with degradation, with Bacteroidota decreasing from approximately 18 % to less than 1 % of the relative abundance of expressed transcripts, while Bacillota increased from approximately 3 % to more than 27 % in the late stage. In parallel, a proportional increase in CAZymes related to cellulose and hemicellulose breakdown was revealed in the late stage, suggesting greater cellulolytic and hemicellulolytic activity at later time points. Notably, a substantial fraction of transcripts identified as key lignocellulases (&gt;30 %) were affiliated with the genus <em>Paenibacillus</em> (phylum Bacillota), highlighting its significant role in biomass degradation throughout the adaptation period. These findings highlight how prolonged exposure to lignocellulosic substrates selectively increases microbial taxa and enzymatic pathways critical for efficient biomass conversion. Such results are relevant for the development of biotechnological solutions such as enzymatic bioprospecting and industrial optimization of microbial lignocellulose processing, as well as for the development of strategies for the enrichment of high-performance degraders through targeted cultivation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106110"},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano zero-valent iron mediated optimization of microalgae-based systems for efficient nutrient and antibiotics removal from aquaculture wastewater","authors":"Chunzhi Zhao ,&nbsp;Bei Lu ,&nbsp;Zhengfang Wang ,&nbsp;Jing Wei ,&nbsp;Yongjun Zhao ,&nbsp;Shoubing Wang","doi":"10.1016/j.ibiod.2025.106114","DOIUrl":"10.1016/j.ibiod.2025.106114","url":null,"abstract":"<div><div>This study was dedicated to investigating the influence of nano zero-valent iron (NZVI) on microalgae-based systems in eliminating nutrients and antibiotics from aquaculture wastewater. The aquaculture industry's fast expansion has caused severe pollution issues, as its wastewater is rich in nutrients and antibiotics. Microalgae-based treatment technologies hold promise, yet the effects of NZVI on their performance remain unclear. Four systems were established with different NZVI concentrations (0 mg L⁻¹, 5 mg L⁻¹, 10 mg L⁻¹,20 mg L⁻¹). Results showed that 10 mg L⁻¹ of NZVI best promoted microalgae growth, and Treatment 2 (<em>Chlorella vulgaris</em> + endophytic bacteria + <em>Clonostachys rosea</em>) performed best. Its removal efficiencies of COD, TN, TP, and tetracycline reached 87.29 ± 8.04 %, 87.76 ± 8.32 %, 88.12 ± 8.45 %, and 99.17 ± 0.52 % respectively. This research identified the optimal technology and NZVI concentration, providing crucial theoretical support for the application of microalgae in aquaculture wastewater treatment. However, it should be noted that real-scale validation is still required to fully confirm its effectiveness.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106114"},"PeriodicalIF":4.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced biodegradation of sulfamethazine in swine manure composting: Pathway elucidation, mechanism insights, and optimization of a novel Bacillus sp. strain","authors":"Xiaojun Lin ,&nbsp;Jingtong Li ,&nbsp;Chunyan Chen ,&nbsp;Zifeng Luo ,&nbsp;Hongxing Tu ,&nbsp;Yuwan Pang ,&nbsp;Xiujuan Wang ,&nbsp;Qianyi Cai ,&nbsp;Zebin Wei ,&nbsp;Jianfeng Huang ,&nbsp;Jingwen Zeng ,&nbsp;Jinrong Qiu","doi":"10.1016/j.ibiod.2025.106112","DOIUrl":"10.1016/j.ibiod.2025.106112","url":null,"abstract":"<div><div>Sulfamethazine (SMZ), a widely used sulfonamide antibiotic frequently detected in livestock waste streams, requires enhanced microbial degradation strategies to prevent its accumulation and associated environmental hazards. While several microbial strains with SMZ degradation capability have been isolated, comprehensive elucidation of metabolic pathways remains incomplete. This study identified <em>Bacillus</em> sp. strain DLY-11, exhibiting exceptional SMZ degradation performance, from thermally treated swine manure compost substrates. Under optimized parameters (5 % (v/v) bacterial inoculum, 59.7 °C operational temperature, pH 8.08 maintained with buffer system, and 0.4 g/L MgSO₄ supplementation), the strain achieved 97.1 % elimination of 20 mg/L SMZ within 48-h incubation. Metabolic pathway analysis through detected intermediates revealed two distinct biodegradation mechanisms involving sulfonamide bond cleavage, oxidative hydroxylation, sulfur dioxide elimination, ring oxidation, amino group substitution, and glucuronic acid conjugation. These findings enhance understanding of sulfonamide antibiotic catabolism while offering a promising microbial resource for controlling veterinary antibiotic contamination in agro-industrial ecosystems.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106112"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vertical distribution and environmental controls of methane cycling processes and associated microbial communities in Lake Chaohu sediments","authors":"Siyan Li ,&nbsp;Zhipeng Wei ,&nbsp;Yaofei Xu ,&nbsp;Aidong Ruan","doi":"10.1016/j.ibiod.2025.106105","DOIUrl":"10.1016/j.ibiod.2025.106105","url":null,"abstract":"<div><div>Methane cycling in eutrophic freshwater lake sediments plays a crucial role in global greenhouse gas emissions. However, the vertical distribution patterns of methane cycling-related microbial processes and their controlling factors remain poorly understood in these ecosystems. Here, we quantified the activity potentials of methane cycling processes in sediments through culture experiments with different treatments, integrating molecular microbiology (metagenomics and qPCR) and sediment characteristics. Results revealed that activity potentials of methane cycling-related processes decreased with sediment depth, while the abundances of genes including <em>mcrA</em>, <em>pmoA</em>, <em>NC10 pmoA</em>, and <em>ANME-2d mcrA</em> showed significantly different patterns in their vertical distribution. A shift from acetotrophic to hydrogenotrophic methanogenesis was observed with increasing depth, occurring at 15–20 cm depth. Moreover, nitrate served as the predominant environmental driver of methane cycling in Lake Chaohu sediments. Significant differences were observed in both community composition and dominant genera of methane cycling-related microorganisms between the 0–20 cm and 20–45 cm sediment layers, with the upper layer dominated by aerobic methane oxidation and the lower layer characterized by nitrate-dependent anaerobic methane oxidation and methanogenesis. This study provides the first comprehensive investigation of methane cycling activity potentials and describes the distribution and environmental controls of methane cycling-related microbes across different sediment depths in a eutrophic freshwater lake. These findings advance our understanding of methane cycling processes in eutrophic lake sediments, providing crucial data and a theoretical foundation for understanding the natural sources and sinks of greenhouse gases, as well as potential strategies for mitigating methane emissions from eutrophic lakes.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106105"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete mineralization of bisphenol F by a newly isolated Microbacterium sp. F2 eliminates its toxicity to sensitive organisms","authors":"Changchang Wang,&nbsp;Qian Li,&nbsp;Kaihua Pan,&nbsp;Qian Zhu,&nbsp;Hongfei Liu,&nbsp;Yanni Huang,&nbsp;Weihao Zhu,&nbsp;Mingliang Zhang,&nbsp;Xin Yan,&nbsp;Qing Hong","doi":"10.1016/j.ibiod.2025.106109","DOIUrl":"10.1016/j.ibiod.2025.106109","url":null,"abstract":"<div><div>Bisphenol F (BPF) is a component of synthetic epoxy resin and polycarbonate plastic. Its residue is frequently detected in water and sediments, posing a threat to aquatic organisms due to the endocrine disrupting effect of BPF. In this study, strain <em>Microbacterium</em> sp. F2, using BPF as the sole carbon source for growth and degrading 98.67 % of 0.10 mM BPF within 24 h, was isolated. Based on the identification of metabolites, strain F2 degraded BPF by the hydroxylation of the bridging carbon atom, and subsequently via oxidation and Baeyer-Villiger reaction to <em>p</em>-hydroxybenzoic acid (PHBA) and 1,4-hydroquinone (HQ), and both of them were further metabolized to provide strain F2 with the carbon source for growth. The half maximal inhibitory concentration (IC50) of BPF, 4,4′-dihydroxybenzophenone (DHBP), 4-hydroxyphenyl 4-hydroxybenzoate (HPHB), HQ and PHBA against <em>Chlorella ellipsoidea</em> at 96 h were 64, 142.5, 205, 325 and 660 μM, respectively. The toxicity of BPF and its metabolites to <em>C. ellipsoidea</em> showed a gradually decreasing trend. The median lethal concentration (LC₅₀) of BPF, DHBP, HPHB, HQ, and PHBA against zebrafish at 96 h were 40.50, 70.31, 71.28, 72.44, and 74.94 μM, respectively. There was no obvious difference in the toxicity of metabolites to zebrafish, but their toxicity was significantly lower than that of BPF. However, the complete mineralization of BPF by strain F2 achieved the detoxification of BPF. This study provides a potential strain for the bioremediation of BPF-polluted environment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106109"},"PeriodicalIF":4.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel multi-drug-resistant yeast efficiently removed ammonia nitrogen from antibiotic-contaminated aquaculture water","authors":"Jie Hu ,&nbsp;Tianhong Cai ,&nbsp;Uli Klümper ,&nbsp;Ling Luo ,&nbsp;Ying Zhu ,&nbsp;Yan He","doi":"10.1016/j.ibiod.2025.106111","DOIUrl":"10.1016/j.ibiod.2025.106111","url":null,"abstract":"<div><div>Aquaculture waters often contain antibiotics, which inhibit the removal of ammonia nitrogen (NH₄⁺-N) by traditional microbial technologies. Thus, it is necessary to find potential microorganisms that can simultaneously tolerate to antibiotics and remove NH₄⁺-N. This study applied a novel isolated multi-drug-resistant yeast strain named <em>Naganishia diffluens</em> Mo to remove NH₄⁺-N in antibiotic-contaminated water. It was found the optimal conditions (glucose as carbon source, pH 7.0, 25 °C, 2 % inoculation, C/N ratio of 10) based on single-factor experiments for <em>Naganishia diffluens</em> Mo removing NH₄⁺-N could achieve 91.6 %. Response surface methodology further opitimized this condition as C/N ratio of 15, 24.9 °C, and pH 6.9, achieving 94.8 % of NH₄⁺-N removal, demonstrating great potentail to remove NH₄⁺-N. Whole-genome analysis indicated that <em>Naganishia diffluens</em> Mo likely removes NH₄⁺-N through ammonia assimilation, utilizing the GDH pathway and the GS-GOGAT metabolic pathway to incorporate NH₄⁺-N into biomass without converting it to nitrate (NO₃⁻) and nitrite (NO₂⁻). Besides, <em>Naganishia diffluens</em> Mo contains multiple antibiotic-resistance genes and genes reponsible for NO₃⁻ and NO₂⁻ removal, suggesting its great potential for N removal in antibiotic-contaminated wastewater.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106111"},"PeriodicalIF":4.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dinitrogen production modes of diverse anammox bacteria and the contribution in nitrogen cycle: A review","authors":"Yiqing Liang ,&nbsp;Peike Gao ,&nbsp;Yuhao Jiao ,&nbsp;Zizhuang Zhang ,&nbsp;Yufeng Xin ,&nbsp;Wenjie Xia ,&nbsp;Ji-Dong Gu","doi":"10.1016/j.ibiod.2025.106113","DOIUrl":"10.1016/j.ibiod.2025.106113","url":null,"abstract":"<div><div>Anaerobic ammonia oxidation (anammox) bacteria are lithoautotrophs coupling the oxidation of ammonium with the reduction of nitrite to produce dinitrogen (N₂). Anammox reaction is one of the important ones in inorganic nitrogen removal. Here, the reported anammox bacteria, nitrogen metabolism, dinitrogen production modes coupled with other microorganisms, occurrence and contribution in diverse ecosystems were comprehensively summarized. A total of 36 species affiliated to 7 genera of anammox bacteria have been reported. Anammox bacteria use nitrite and nitric oxide as electron acceptors and ammonium as electron donor to carry out anammox reaction. Some also have functional genes required for Dissimilatory nitrate reduction to ammonium (DNRA) process. Anammox bacteria can conduct nitrogen removal via coupling with multiple microorganisms, and realized nitrogen removal via coupling with iron reduction, sulfate reduction, heterotrophic denitrification, sulfur autotrophic denitrification, partial nitrification, denitrifying anaerobic methane oxidation. However, the mechanisms and construction of efficient symbiotic systems consisting of anammox bacteria and other microorganisms is still unclear. Anammox is widespread in ecosystems, and contributes considerable nitrogen yield. Quantification of the nitrogen loss contributed by anammox bacteria in typical ecosystems is an issue in future. Collectively, bacteria have diverse carbon and nitrogen metabolic pathways, and can remove reactive nitrogen species via coupling with other microorganisms, showing a complex and critical robust roles in nitrogen transformation in diverse ecosystems.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106113"},"PeriodicalIF":4.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dissipation of Di (2-ethylhexyl) phthalate in soil with different moisture: A comprehensive analysis of its relationship with microbial community structure","authors":"Fuying Zhang ,&nbsp;Lulu Sun ,&nbsp;Yujiao Song,&nbsp;Guohan Wu,&nbsp;Qizheng Zhu,&nbsp;Zhengyu Zhang,&nbsp;Xuezhu Zhu ,&nbsp;Baitao Lv","doi":"10.1016/j.ibiod.2025.106107","DOIUrl":"10.1016/j.ibiod.2025.106107","url":null,"abstract":"<div><div>Phthalate esters (PAEs) have become an increasing pollutant in agricultural soils, and soil moisture plays a crucial role in influencing the dissipation of PAEs in soil. Di (2-ethylhexyl) phthalate (DEHP) was selected as a representative of PAEs in the soil microcosm experiment to investigate the effects of soil moisture on the dissipation of PAEs. Soil microorganisms were analyzed by phospholipid fatty acids (PLFAs) and DNA-based (bacteria 16S rRNA gene and fungi ITS gene) high-throughput sequencing to reveal the relationships between microbial communities and PAEs dissipation. The results showed that 60 % water-holding capacity (WHC) was an appropriate moisture for DEHP dissipation. According to the PLFAs analysis, DEHP significantly increased the total microorganism biomass and the growth of actinomycetes, and it significantly decreased the <em>H</em> value and the growth of gram (−) bacteria. Significant correlations were found between soil moisture and the biomass of fungi, gram (−) bacteria, gram (+) bacteria, anaerobe, and actinomycetes. The fungal biomass was higher in the soils with 60 % WHC than those in the soils with 100 % WHC. The relative abundance of DEHP-degrading bacteria (<em>Sphingoaurantiacus, Sphingomonas, Lysobacter,</em> and <em>Arthrobacter</em>) and DEHP-degrading fungi (<em>Fusarium</em>) were higher in the soils with 60 % WHC than those in the soils with 100 % WHC, based on DNA-based high-throughput sequencing analysis. Meanwhile, significant positive correlations were observed between the dissipation of DEHP and the abundance of degrading microorganisms. DEHP significantly decreased the bacterial diversity but increased the richness of bacteria and actinomycetes, as well as the relative abundance of DEHP-degrading bacteria (<em>Proteobacteria, Sphingomonas, Gemmatimonas,</em> and <em>Ramlibacter</em>) and fungi (<em>Fusarium</em> and <em>Talaromyces</em>).</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106107"},"PeriodicalIF":4.1,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustained protection of stone cultural relics: Utilizing antiviral oseltamivir to effectively eliminate lichen and diseases on artifacts","authors":"Fengchang Li ,&nbsp;Ning Wang ,&nbsp;Xuan Zhang ,&nbsp;Jiren Hui ,&nbsp;Qinya Ge ,&nbsp;Zhenbin Xie ,&nbsp;Jiangchun Wei ,&nbsp;Rongxiang Fang ,&nbsp;Zhijian Liang ,&nbsp;Xinli Wei ,&nbsp;Jian Ye","doi":"10.1016/j.ibiod.2025.106106","DOIUrl":"10.1016/j.ibiod.2025.106106","url":null,"abstract":"<div><div>Saxicolous lichens, which colonize rock substrates, play a major role in the deterioration of stone cultural relics. Traditional biocides, including antibiotics and antifungal agents, pose risks of resistance development and biofouling, underscoring the need for effective and a long-term treatment against biodeterioration. In this study, we discovered a novel double-stranded RNA (dsRNA) virus, Pyxine petricola partitivirus 1 (PpPV1), in saxicolous lichens collected from the fallen stones of the Niujiaozhai grottoes in Sichuan Province, China. This virus was found in 70.2% of saxicolous lichens from a global sample of 118 lichens, including those from notable sites, including Angkor sandstone monuments in Cambodia and the Great Wall of China. We developed a biocide strategy using the antiviral oseltamivir, which significantly reduced the viral loading and the photosynthetic activity of the lichen-forming algae, impairing lichen functionality. This innovative approach not only offers a novel method for protecting stone cultural relics but also contributes to both biocontrol and heritage conservation by safeguarding against viral pathogens and mitigating the biological damage caused by lichens.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106106"},"PeriodicalIF":4.1,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of coating defects, cathodic protection and Pseudomonas aeruginosa on the corrosion behavior of EH40 steel","authors":"Xue Zhang,&nbsp;Yanjun Liu,&nbsp;Shiqiang Chen,&nbsp;Xin Cheng,&nbsp;Guangzhou Liu","doi":"10.1016/j.ibiod.2025.106108","DOIUrl":"10.1016/j.ibiod.2025.106108","url":null,"abstract":"<div><div>The combination of coatings and cathodic protection (CP) is commonly used for corrosion protection of marine metal materials. However, coating failure is sometimes unavoidable in practical application. Previous works have found that effect of CP will be reduced when defects occur, and the presence of microorganisms will significantly affect the corrosion process of substrate metal. However, it remains unknown whether the size of the defect influences the efficacy of CP in <em>Pseudomonas aeruginosa</em> medium (seawater modified Luria-Bertani medium at 37 °C under aerobic condition). In this work, coating defects with diameters of 0.2 mm, 1.0 mm and 2.0 mm were manufactured on surface of EH40 steel using a carbon dioxide laser. The electrochemical and surface analysis results show that CP of −0.85 V_CSE can not protect EH40 steel with different coating defects in <em>P. aeruginosa</em> seawater. Especially for coating defects of 2.0 mm, EH40 steel is polarized anodically. However, CP of −0.95 V_CSE can protect EH40 steel with the different defects. The results of fluorescence microscope and the oxidative stress indicate that the growth and adhesion of <em>P. aeruginosa</em> are inhibited on surface of EH40 steel, which is due to that CP of −0.95 V_CSE exerts stronger oxidative stress on <em>P. aeruginosa</em>. The findings of this study provide valuable insights into the effects of coating defects, CP and <em>P. aeruginosa</em> on the corrosion of EH40 steel in seawater, and guidance for dealing with coating failure in engineering and selecting suitable protection measures for marine equipment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106108"},"PeriodicalIF":4.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}