International Biodeterioration & Biodegradation最新文献

{"title":"The dissipation of Di (2-ethylhexyl) phthalate in soil with different moisture: A comprehensive analysis of its relationship with microbial community structure","authors":"Fuying Zhang ,&nbsp;Lulu Sun ,&nbsp;Yujiao Song,&nbsp;Guohan Wu,&nbsp;Qizheng Zhu,&nbsp;Zhengyu Zhang,&nbsp;Xuezhu Zhu ,&nbsp;Baitao Lv","doi":"10.1016/j.ibiod.2025.106107","DOIUrl":"10.1016/j.ibiod.2025.106107","url":null,"abstract":"<div><div>Phthalate esters (PAEs) have become an increasing pollutant in agricultural soils, and soil moisture plays a crucial role in influencing the dissipation of PAEs in soil. Di (2-ethylhexyl) phthalate (DEHP) was selected as a representative of PAEs in the soil microcosm experiment to investigate the effects of soil moisture on the dissipation of PAEs. Soil microorganisms were analyzed by phospholipid fatty acids (PLFAs) and DNA-based (bacteria 16S rRNA gene and fungi ITS gene) high-throughput sequencing to reveal the relationships between microbial communities and PAEs dissipation. The results showed that 60 % water-holding capacity (WHC) was an appropriate moisture for DEHP dissipation. According to the PLFAs analysis, DEHP significantly increased the total microorganism biomass and the growth of actinomycetes, and it significantly decreased the <em>H</em> value and the growth of gram (−) bacteria. Significant correlations were found between soil moisture and the biomass of fungi, gram (−) bacteria, gram (+) bacteria, anaerobe, and actinomycetes. The fungal biomass was higher in the soils with 60 % WHC than those in the soils with 100 % WHC. The relative abundance of DEHP-degrading bacteria (<em>Sphingoaurantiacus, Sphingomonas, Lysobacter,</em> and <em>Arthrobacter</em>) and DEHP-degrading fungi (<em>Fusarium</em>) were higher in the soils with 60 % WHC than those in the soils with 100 % WHC, based on DNA-based high-throughput sequencing analysis. Meanwhile, significant positive correlations were observed between the dissipation of DEHP and the abundance of degrading microorganisms. DEHP significantly decreased the bacterial diversity but increased the richness of bacteria and actinomycetes, as well as the relative abundance of DEHP-degrading bacteria (<em>Proteobacteria, Sphingomonas, Gemmatimonas,</em> and <em>Ramlibacter</em>) and fungi (<em>Fusarium</em> and <em>Talaromyces</em>).</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106107"},"PeriodicalIF":4.1,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustained protection of stone cultural relics: Utilizing antiviral oseltamivir to effectively eliminate lichen and diseases on artifacts","authors":"Fengchang Li ,&nbsp;Ning Wang ,&nbsp;Xuan Zhang ,&nbsp;Jiren Hui ,&nbsp;Qinya Ge ,&nbsp;Zhenbin Xie ,&nbsp;Jiangchun Wei ,&nbsp;Rongxiang Fang ,&nbsp;Zhijian Liang ,&nbsp;Xinli Wei ,&nbsp;Jian Ye","doi":"10.1016/j.ibiod.2025.106106","DOIUrl":"10.1016/j.ibiod.2025.106106","url":null,"abstract":"<div><div>Saxicolous lichens, which colonize rock substrates, play a major role in the deterioration of stone cultural relics. Traditional biocides, including antibiotics and antifungal agents, pose risks of resistance development and biofouling, underscoring the need for effective and a long-term treatment against biodeterioration. In this study, we discovered a novel double-stranded RNA (dsRNA) virus, Pyxine petricola partitivirus 1 (PpPV1), in saxicolous lichens collected from the fallen stones of the Niujiaozhai grottoes in Sichuan Province, China. This virus was found in 70.2% of saxicolous lichens from a global sample of 118 lichens, including those from notable sites, including Angkor sandstone monuments in Cambodia and the Great Wall of China. We developed a biocide strategy using the antiviral oseltamivir, which significantly reduced the viral loading and the photosynthetic activity of the lichen-forming algae, impairing lichen functionality. This innovative approach not only offers a novel method for protecting stone cultural relics but also contributes to both biocontrol and heritage conservation by safeguarding against viral pathogens and mitigating the biological damage caused by lichens.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106106"},"PeriodicalIF":4.1,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of coating defects, cathodic protection and Pseudomonas aeruginosa on the corrosion behavior of EH40 steel","authors":"Xue Zhang,&nbsp;Yanjun Liu,&nbsp;Shiqiang Chen,&nbsp;Xin Cheng,&nbsp;Guangzhou Liu","doi":"10.1016/j.ibiod.2025.106108","DOIUrl":"10.1016/j.ibiod.2025.106108","url":null,"abstract":"<div><div>The combination of coatings and cathodic protection (CP) is commonly used for corrosion protection of marine metal materials. However, coating failure is sometimes unavoidable in practical application. Previous works have found that effect of CP will be reduced when defects occur, and the presence of microorganisms will significantly affect the corrosion process of substrate metal. However, it remains unknown whether the size of the defect influences the efficacy of CP in <em>Pseudomonas aeruginosa</em> medium (seawater modified Luria-Bertani medium at 37 °C under aerobic condition). In this work, coating defects with diameters of 0.2 mm, 1.0 mm and 2.0 mm were manufactured on surface of EH40 steel using a carbon dioxide laser. The electrochemical and surface analysis results show that CP of −0.85 V_CSE can not protect EH40 steel with different coating defects in <em>P. aeruginosa</em> seawater. Especially for coating defects of 2.0 mm, EH40 steel is polarized anodically. However, CP of −0.95 V_CSE can protect EH40 steel with the different defects. The results of fluorescence microscope and the oxidative stress indicate that the growth and adhesion of <em>P. aeruginosa</em> are inhibited on surface of EH40 steel, which is due to that CP of −0.95 V_CSE exerts stronger oxidative stress on <em>P. aeruginosa</em>. The findings of this study provide valuable insights into the effects of coating defects, CP and <em>P. aeruginosa</em> on the corrosion of EH40 steel in seawater, and guidance for dealing with coating failure in engineering and selecting suitable protection measures for marine equipment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106108"},"PeriodicalIF":4.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Trichoderma atroviride G79/11 growth intensity and metabolic activity under different additives using Biolog system","authors":"Mohsin Ali,&nbsp;Justyna Cybulska,&nbsp;Artur Zdunek,&nbsp;Magdalena Frąc","doi":"10.1016/j.ibiod.2025.106104","DOIUrl":"10.1016/j.ibiod.2025.106104","url":null,"abstract":"<div><div><em>Trichoderma</em> species have the ability to act as a safe and sustainable environmental way to enhance crop productivity and deal with pathogenic diversity. It is a crucial task to evaluate and boost their growth intensity and metabolic activity through an efficient and accurate method with novel nutritional sources. In the current study, Biolog MT2 microplates were used to evaluate the <em>T</em>. <em>atroviride</em> G79/11 growth intensity and metabolic activity in the presence of polysaccharide additives such as pectin fractions (water-soluble pectin-WSP, diluted alkali-soluble pectin-DASP, and oxalate soluble pectin-OSP) and bacterial cellulose (BC), fortified with inorganic salts. Pectin, as a significant component of fruit and vegetable industry waste, and bacterial cellulose, which has an exceptional water retention capacity, were used for the first time to stimulate the growth of <em>T. atroviride</em> G79/11. The highest metabolic efficiency of <em>T. artoviride</em> G79/11 was observed in WSP and OSP fractions supplemented with Ca, Zn, Mg and Fe. Glucose was recorded as a dominant constituent of BC composition, whereas galacturonic acid was detected with the highest amount of 84.53 %, 7.56 %, and 71.70 % of OSP, WSP, and DASP fractions, respectively. Applied polysaccharide solutions significantly differed in viscosity, which was also influenced by the sterilization process. The strain showed significantly high growth intensity and metabolic activity in potato dextrose broth amended with WSP, BC, ZnCl₂, saccharose, glucose, starch, and a combination of OSP with CaCl₂ additive as compared to its respective groups and combinations as well as FF-IF. This study emphasizes that natural polysaccharides and their possible composites can boost the growth intensity and metabolic activities of the fungal strain.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106104"},"PeriodicalIF":4.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The degradation of nicotinic acid is controlled by MarR-family transcriptional regulator NicR in Alcaligenes faecalis","authors":"Siqiong Xu ,&nbsp;Cuiwei Chu ,&nbsp;Xinyang Li ,&nbsp;Yongli Wang ,&nbsp;Hongfei Zhang ,&nbsp;Lili Li ,&nbsp;Lixia Zhu ,&nbsp;Yongchuang Liu ,&nbsp;Shuhua Fan ,&nbsp;Yuehui Tang ,&nbsp;Keshi Ma","doi":"10.1016/j.ibiod.2025.106103","DOIUrl":"10.1016/j.ibiod.2025.106103","url":null,"abstract":"<div><div>Nicotinic acid (NA), or vitamin B3, is a natural pyridine carboxylic derivative, a precursor for numerous essential biological molecules. Previous studies have demonstrated that microorganisms are essential in NA degradation, exhibiting high genetic and metabolic diversity. This study investigated the biodegradation and regulatory properties of NA by the <em>Alcaligenes faecalis</em> JQ135 and the diversity and interrelationships of microbial metabolism of NA. NicR functions as a repressor in the <em>nic</em> cluster. An electrophoretic mobility shift assay (EMSA) demonstrated that NicR can bind to the promoter regions of R, C, B, A, and P operons in strain JQ135, with these multiple binding sites exhibiting the consensus sequence 5′-GTNNAC-3'. Bioinformatics analysis revealed 24 distinct <em>nic</em> cluster order types. The <em>nic</em> gene cluster in strain JQ135 comprises 15 genes arranged in two distantly located fragments and transcribed as five operons. These configurations are conserved within the genus <em>Alcaligenes</em> and are unique compared to the other 23 order types. The functional correlation among NicRs with differing degrees of similarity was examined, revealing that amino acid residues Q64 and V103 are crucial for NicR regulation. This study offers new molecular insights into the various regulatory mechanisms of bacterial degradation of NA in nature.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106103"},"PeriodicalIF":4.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling fungal degradation pathways for polyurethane and polyethylene through enrichment cultures and metabolic analysis","authors":"Eyalira Jacob Okal ,&nbsp;Jie Zhou ,&nbsp;Yanfei Wu ,&nbsp;Tingfang Zhong ,&nbsp;Yue Tang ,&nbsp;Zhengyu Sun ,&nbsp;Ruifang Xu ,&nbsp;Yuwei Hu ,&nbsp;Na Hu ,&nbsp;Jingxian Li ,&nbsp;Samantha C. Karunarathna ,&nbsp;Peter E. Mortimer ,&nbsp;Shahid Iqbal ,&nbsp;Dongmei Yu ,&nbsp;Jianchu Xu ,&nbsp;Heng Gui","doi":"10.1016/j.ibiod.2025.106097","DOIUrl":"10.1016/j.ibiod.2025.106097","url":null,"abstract":"<div><div>The increasing accumulation of plastics in the environment poses significant threats to marine and terrestrial ecosystems. However, recent research highlights the potential of <em>Lasiodiplodia iranensis</em>, a tropical ascomycete fungus, to degrade synthetic plastics. The intrinsic molecular mechanisms and metabolic responses during the interaction and hydrolysis of plastics, particularly for polyurethane (PU) and polyethylene (PE), remain largely unexplored. This study was conducted to investigate its degradation activity and metabolic responses to PU and PE and showed <em>L. iranensis</em> could colonise and significantly degrade PU (11.05 % weight loss), showcasing its impressive capabilities but having minimal effect on commercial PE (0.53 %) in 60 days. Metabolomic analysis identified 51 and 63 differentially expressed metabolites in response to PU and PE, respectively, with 30 common metabolites. Pathways for enzyme production, metal ion chelation, nutrient uptake, and Krebs cycle intermediates were activated in the fungus exposed to PU, likely contributing to its enhanced hydrolysis of PU. In contrast, pathways for stress response, antioxidant activity, signal transduction, and membrane integrity were predominant for PE, likely due to its limited degradability. Increased metabolism of compounds like 2-oxoarginine, proline, L-valine and 1-methyl histidine, which serve as carbon and nitrogen sources, osmoprotectants, and derivatives for fungal enzymes were observed in both treatments, thus supporting nutrient and enzyme synthesis. Hydrolytic and oxidative enzymes, mainly esterase, lipase, cutinase, laccase, and peroxidase, were implicated in PU and PE biodegradation, with PU showing more robust degradation potential. This study provides useful insights into the metabolic pathways that facilitate plastic degradation in <em>L. iranensis</em>, identifying potential fungal metabolites and enzymes that could be harnessed for bioremediation efforts, thereby advancing the development of fungal-based solutions for plastic waste reduction.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106097"},"PeriodicalIF":4.1,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the genomic and metabolic mechanisms of pyrene and phenanthrene degradation by Mycolicibacterium sp. SCSIO 43805: A high-Efficiency bacterium isolated from seagrass sediment","authors":"Manzoor Ahmad ,&nbsp;Tongyin Liang ,&nbsp;Yuhang Zhang ,&nbsp;Youshao Wang ,&nbsp;Jidong Gu ,&nbsp;Hao Cheng ,&nbsp;Khaled Masmoudi ,&nbsp;Weiguo Zhou ,&nbsp;Qingsong Yang ,&nbsp;Xiaofang Huang ,&nbsp;Junde Dong ,&nbsp;Juan Ling","doi":"10.1016/j.ibiod.2025.106101","DOIUrl":"10.1016/j.ibiod.2025.106101","url":null,"abstract":"<div><div>The ability of microorganisms to break down persistent organic pollutants in the environment has sparked significant interest in bioremediation, as it provides a firm foundation for implementing an effective, robust, and eco-friendly approach. In this study, we isolated a bacterium, identified as <em>Mycolicibacterium</em> sp. SCSIO 43805, from seagrass sediments and studied its potential for degrading pyrene and phenanthrene. The stain effectively degraded pyrene and phenanthrene, both provided at a concentration of 50 mg/L, within 12 days of incubation, as evidenced by the absence of any noticeable peaks related to pyrene and phenanthrene in the GC-MS profile of the 12-day extract. Moreover, the whole genome sequence analysis unveiled a comprehensive array of genes crucial for the complete degradation of polycyclic aromatic hydrocarbons. Multiple copies of dioxygenases such as <em>nidA</em>, <em>nidB</em> and <em>nidD</em> and monooxygenase such as cytochrome P450, which are essential for initial cleavage of aromatic rings, were detected in the genome of the <em>Mycolicibacterium</em> sp. SCSIO 43805. Furthermore, based on the gene contents and metabolic profiling of the extracts, we speculated that <em>Mycolicibacterium</em> sp. SCSIO 43805 could degrade pyrene, phenanthrene and other PAHs via phthalate and β-Ketoadipate pathway. The comparative genomics with other member of the genus <em>Mycolicibacterium</em> showed that <em>Mycolicibacterium</em> sp. SCSIO 43805 possessed a maximum number of genes involved in polycyclic aromatic hydrocarbon degradation. Hence, based on results of genomic, comparative genomics and metabolic profiling, <em>Mycolicibacterium</em> sp. SCSIO 43805 demonstrated high ability in degrading persistent organic pollutants and is an excellent biological agent for bioremediation of contaminated environment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106101"},"PeriodicalIF":4.1,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the impact of agrifood byproduct-based bioplastics on soil microbial communities and functioning","authors":"Marija Prodana ,&nbsp;Catarina Malheiro ,&nbsp;Joana Lopes ,&nbsp;Sara Peixoto ,&nbsp;Rui G. Morgado ,&nbsp;Idalina Gonçalves ,&nbsp;Paula Ferreira ,&nbsp;Manuel A. Coimbra ,&nbsp;Artur Alves ,&nbsp;Micael F.M. Gonçalves ,&nbsp;Sandra Hilário ,&nbsp;Susana Loureiro","doi":"10.1016/j.ibiod.2025.106083","DOIUrl":"10.1016/j.ibiod.2025.106083","url":null,"abstract":"<div><div>The environmental burden of plastic pollution has driven the search for sustainable alternatives, such as biodegradable bioplastics derived from agrifood byproducts, which hold the potential for addressing global waste management challenges. Given the close relationship between the biodegradability of biopolymers and soil microbial activities, it is vital to understand how the presence of novel bioplastics affects their function and structure. This study assessed microbial responses to different plastics: potato starch-based bioplastic, locust bean-based bioplastic, and non-biodegradable polyamide-polyethylene plastic in natural sandy-loam soil (LUFA 2.2). Soil without any (bio)plastics was used as a control. Despite initial pronounced differences in defragmentation rates between the two types of bioplastics, only 10 and 20 % w/w of the fragments were recovered after 17 weeks. The type of bioplastic influenced the fungal colonization pattern, with potato starch-based bioplastic resulting in more isolated fungal species than locust bean-based bioplastic. The soil dehydrogenase response was inconsistent, while β-glucosidase activity showed an initial increase in both bioplastic treatments, with a sustained stimulation only in potato starch-based bioplastic. β-glucosidase activity coincided with higher carbon substrate consumption maintained by the end of the 17 weeks of exposure, indicating more dynamic changes in microbial functions in potato starch-based bioplastic. Decreased carbon substrate consumption was observed in non-biodegradable polyamide-polyethylene plastic. The current study represents a first screening approach for the impact of applying agrifood byproducts as novel biopolymers directly in the soil, demonstrating their distinct effects without any pronounced adverse effects on soil microbiota compared to conventional polyamide-polyethylene plastic.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106083"},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reaction zone evolution and remediation efficacy evaluation of in-situ biogeochemical transformation of emulsified vegetable oil-FeSO4 enhanced tetrachloroethylene-contaminated aquifers","authors":"Chen Sun ,&nbsp;Jun Dong","doi":"10.1016/j.ibiod.2025.106100","DOIUrl":"10.1016/j.ibiod.2025.106100","url":null,"abstract":"<div><div>Enhanced in-situ biogeochemical transformation (ISBGT) has been proven effective in promoting the abiotic β-elimination of chlorinated solvents. However, the mechanisms underlying reaction zone evolution, remediation efficiency, and long-term permeability changes during the remediation process remain poorly understood. This study employed emulsified vegetable oil (EVO) and FeSO₄ as amendments to establish an in-situ reaction zone in a simulated column system. The reaction zone evolution was systematically analyzed, and the remediation efficiency and permeability variations in a PCE-contaminated aquifer were assessed. The results showed that after a single injection of EVO-FeSO₄, the reaction zone evolved through three distinct stages, including emulsified oil decomposition, microbial reduction, and β-elimination. The formation and aging mechanisms of the sulfur-iron mineral biogeobattery were also clarified. During the 300-day experimental period, the system achieved a PCE removal efficiency of 93.3%, with abiotic degradation processes contributing 96.36% of the total removal. This study provides important insights for the further development and practical application of ISBGT technology.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106100"},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation pathways of phthalate esters by Mycolicibacterium parafortuitum J101 and its ability to enhance bioremediation","authors":"Chatsuda Sakdapetsiri ,&nbsp;Chavisa Jeerasantikul ,&nbsp;Ritu Ningthoujam ,&nbsp;Adisan Rungsihiranrut ,&nbsp;Onruthai Pinyakong","doi":"10.1016/j.ibiod.2025.106085","DOIUrl":"10.1016/j.ibiod.2025.106085","url":null,"abstract":"<div><div>Phthalate esters (PAEs) are recalcitrant pollutants commonly used as plasticizers, and their degradation most effectively occurs by microorganisms. However, specific aspects of the degradation mechanism, particularly enzyme specificity and catalytic processes in the upper degradation pathway, remain unclear. Additionally, genes encoding esterases responsible for initial PAE hydrolysis have not been conclusively identified in many bacterial genera. In this study, <em>Mycolicibacterium parafortuitum</em> strain J101 was found to be capable of efficiently degrading and mineralizing three PAEs at an initial concentration of 100 mg/L in 7 days: short-chain dimethyl phthalate (88.57 %), diethyl phthalate (96.83 %), and dibutyl phthalate (99.37 %). Environmental conditions such as a pH less than 6 and greater than 5 % salinity affect the PAE degradation efficiency of strain J101. Genome mining revealed genes potentially involved in the complete degradation of PAEs by J101, which corresponds with J101's observed metabolic activity and gene expression. The <em>cut001</em>9 gene encodes a cutinase enzyme that is capable of hydrolyzing PAEs for initial degradation in the upper pathway. PAEs are transformed to phthalic acid, subsequently converted to protocatechuate and ultimately transformed to CO₂ and H₂O. A microcosm study revealed that the introduction of strain J101 significantly enhanced the efficiency of the degradation of mixed PAEs, and the strain synergized with indigenous microorganisms. Furthermore, the addition of strain J101 in landfill soil increased the diversity and complexity of interactions within the landfill soil bacterial community. Consequently, strain J101 demonstrates significant potential for application in the bioremediation of PAE-polluted environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"202 ","pages":"Article 106085"},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}