Minkee Cho , Junbeom Jeon , Jaai Kim , Sugeun Go , Heesuk Jung , Sang-Leen Yun , Hyokwan Bae
{"title":"Biofertilization using microalgae-bacteria consortium for phenolic stress mitigation of sprouted barley: Functional genes profiling of soil bacteria","authors":"Minkee Cho , Junbeom Jeon , Jaai Kim , Sugeun Go , Heesuk Jung , Sang-Leen Yun , Hyokwan Bae","doi":"10.1016/j.ibiod.2025.106070","DOIUrl":"10.1016/j.ibiod.2025.106070","url":null,"abstract":"<div><div>As alternatives to conventional chemical fertilizers, biofertilizers have gained attention, in particular, microalgae-bacteria co-culture biofertilizers (MBCFs) are promising candidates owing to their synergistic interactions. Excessive sludge has been considered a sustainable source of MBCFs due to its proper nutrient composition and biodiversity. This study investigated the feasibility of MBCFs in promoting plant growth. The sprouted barley, selected as a model plant, was exposed to the phenolic stress, and its alleviatory effect by MBCFs was evaluated. The plant's growth characteristics such as germination rate, weight, and length were enhanced after adding MBCFs even under phenol exposure. <em>Desmodesmus</em> and <em>Polaromonas</em> were predominant microalgae and bacteria genus, respectively, and those are attributed to possess the phenol-degrading and plant-promoting properties. The bacterial functional gene prediction revealed several putative genes, including <em>gst</em> (Glutathione R-transferase) and <em>exb</em>B (Iron complex outermembrane recepter protein), which act to promote plant growth. This study suggests that the protective and promotive functions of MBCFs enhance the growth of sprouted barley and alleviate phenolic stress.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106070"},"PeriodicalIF":4.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Evangelina Pareja-Sánchez , Antonio Jesús García-Moreno , Melchor Martínez-García , Lola Pérez-Colodrero , Laura García-Zapata , Roberto García-Ruiz
{"title":"Optimizing alperujo composting strategies for acceleration and quality enhancement","authors":"Evangelina Pareja-Sánchez , Antonio Jesús García-Moreno , Melchor Martínez-García , Lola Pérez-Colodrero , Laura García-Zapata , Roberto García-Ruiz","doi":"10.1016/j.ibiod.2025.106076","DOIUrl":"10.1016/j.ibiod.2025.106076","url":null,"abstract":"<div><div>Alperujo (AL), the main byproduct of olive oil production, holds promise for circular economy applications, particularly as a soil amendment. However, composting AL faces challenges, including small-scale facilities and lengthy processes often exceeding four months, increasing costs and limiting capacity. This study evaluated thirteen treatments to accelerate and enhance composting, using AL at seventy percent wet weight mixed with olive leaf as a bulking agent along with various activators and accelerators, including manures, biochar, ash, olive pomace, wood vinegar, and four commercial products with microorganisms. The composting mixture comprised 30 kg wet weight of materials. Temperature, moisture, pH, total organic matter, total nitrogen, phosphorus, potassium, C/N ratio, phytotoxicity, and germination were monitored. Composting duration ranged from twenty-seven to eighty-four days, with chicken manure and ash mixtures achieving the shortest times, between twenty and twenty-seven days. Initial pH and its daily increase were key factors in optimizing composting efficiency, correlating with shorter durations (r between −0.65 and −0.70). The highest-quality composts were obtained with cow, goat, and chicken manure, all surpassing two percent total nitrogen and exhibiting C/N ratios ranging from 14.6 to 26.1. Mean sunflower and pea biomass production and nitrogen use efficiency were between 1.43 to 1.87 times and 1.96 to 4.12 times higher, respectively, for compost enriched with microorganisms, demonstrating their effectiveness in supporting plant development. Composts, except those with ashes, met regulatory limits for heavy metals and polyphenols, confirming their safety and suitability as organic fertilizers. Optimized AL composting mixtures can produce high-quality, eco-friendly amendments, shorten composting times, and improve plant growth.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106076"},"PeriodicalIF":4.1,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shichong Zhao, Bin Hou, Kunchan Zhang, Yating Jia, Jing Lu
{"title":"Biogenic palladium nanoparticles reclaimed by a novel strain Citrobacter braakii Z1 accelerating RDX biodegradation in the munition effluent","authors":"Shichong Zhao, Bin Hou, Kunchan Zhang, Yating Jia, Jing Lu","doi":"10.1016/j.ibiod.2025.106075","DOIUrl":"10.1016/j.ibiod.2025.106075","url":null,"abstract":"<div><div>The microbial degradation of high-energy explosives has emerged as a cost-effective environmental remediation strategy, but is hindered by low degradation efficiency due to the slow electron transfer. Biogenic Pd<sup>0</sup> nanoparticles (bio-Pd<sup>0</sup>) demonstrate dual functionality, maintaining catalytic activity for N-NO<sub>2</sub> bond reduction while substantially enhancing electron transfer efficiency. This study introduces an innovative single-cell system that synergistically combines chemical catalysis with biological enhancement through in situ synthesis of nanoparticles. Comprehensive characterization through electron microscopy, spectroscopic analysis, and electrochemical measurements revealed that bio-Pd<sup>0</sup> synthesized by <em>Citrobacter braakii</em> Z1 (<em>C. braakii</em> Z1) were preferentially localized in the periplasmic space and extracellular matrix, forming a stable hybrid named bio-Pd<sup>0</sup>@<em>C. braakii</em> Z1. This process relied on the bio-reduction mediated by biohydrogen and extracellular electron transfer processes via c-type cytochromes (c-Cyts) and flavins. Notably, RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) degradation efficiency increased from 11 % with microbes alone to nearly 100 % with bio-Pd<sup>0</sup>. Although the observed RDX degradation in inactivated bio-Pd<sup>0</sup>@<em>C. braakii</em> Z1 and the detected hydrogenolysis intermediates indicated the participation of chemical catalysis, the negative correlation between RDX degradation rate with the Pd:biomass ratios suggested the biology process dominated RDX degradation. Meanwhile, the higher output current in bio-Pd<sup>0</sup>@<em>C. braakii</em> Z1 and the extracellular electron consumption by RDX indicated the bio-Pd<sup>0</sup>-mediated extracellular biodegradation was the primary driver for improved RDX removal efficiency. Toxicity evaluation confirmed that the in-situ combination of Pd-catalyzed chemical destruction and biological enhancement in a single cell is a feasible and environmentally friendly strategy for RDX removal with a reduced ecological risk.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106075"},"PeriodicalIF":4.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Danika Nicoletti , Bei Yin , Jordan Schmidt , Kenneth Wunch , Lisa Gieg , Gary Jenneman
{"title":"Metabolic stress induced by nitrite enhances biocide kill of sulfate reducing bacteria in oilfield enrichments","authors":"Danika Nicoletti , Bei Yin , Jordan Schmidt , Kenneth Wunch , Lisa Gieg , Gary Jenneman","doi":"10.1016/j.ibiod.2025.106067","DOIUrl":"10.1016/j.ibiod.2025.106067","url":null,"abstract":"<div><div>Biocides are commonly employed in oilfield settings to mitigate the activity of sulfate reducing bacteria (SRB) that cause biofouling, reservoir souring and microbiologically influenced corrosion. Key challenges of applying biocides in the oilfield include their environmental toxicity and the need to apply high concentrations to overcome their deactivation and degradation by chemical and physical conditions. One strategy to reduce biocide dosages and ecotoxicity is to apply chemicals that enhance their activity. Nitrite, a known metabolic inhibitor of SRB, was previously found to be synergistic in inhibiting sulfate reduction when used in combination with glutaraldehyde. In the present study, pre-treatment of an SRB enrichment with 2.0 mM nitrite to lower cellular ATP was found to enhance the biocidal activity of glutaraldehyde. These results revealed a linear relationship exists between the decrease in adenylate energy charge of an SRB enrichment and a 10,000-fold increase in planktonic SRB kill, supporting a mechanism that metabolic stress imposed by nitrite enhances the biocidal activity of glutaraldehyde. Furthermore, the results showed increasing nitrite pre-treatment times support lower doses of glutaraldehyde needed to achieve enhanced SRB kill while a nitrite concentration of less than 0.04 mM was effective suggesting nitrite is a very efficient biocide enhancer.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106067"},"PeriodicalIF":4.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kangning Dong , Xiuxia Zhang , De Li , Kang Xiong , Lihan Ren , Mingbo Sun , Zhengyong Lv
{"title":"Rapid removal of 2,4-DCP by activated carbon loaded nano-zero valence iron immobilized microorganisms","authors":"Kangning Dong , Xiuxia Zhang , De Li , Kang Xiong , Lihan Ren , Mingbo Sun , Zhengyong Lv","doi":"10.1016/j.ibiod.2025.106071","DOIUrl":"10.1016/j.ibiod.2025.106071","url":null,"abstract":"<div><div>PEG-nZVI/GAC@<em>B</em> was used to repair 2,4-DCP contamination which was difficult to solve by traditional physical, chemical and biological methods. Characterization techniques confirm the successful incorporation of nZVI particles and <em>Bacillus marisflavi</em> into the GAC's surface folds and pores. The polyethylene glycol 4000 (PEG-4000) coating prevents the formation of Fe<sup>3+</sup> on nZVI surfaces. Compared to standard GAC, PEG-nZVI/GAC@<em>B</em> demonstrates higher electron transfer efficiency. Key parameters including nZVI content, PEG/nZVI mass ratio, and immobilization time were optimized for maximum efficiency. PEG-nZVI/GAC@<em>B</em> also shows effectiveness in removing other chlorophenol compounds. The removal process primarily involves GAC adsorption, followed by <em>Bacillus marisflavi</em> degradation and nZVI-induced reductive dechlorination. Analyses using IC and GC-MS reveal rapid dechlorination of 2,4-DCP adsorbed on immobilized microorganism by nZVI, with <em>Bacillus marisflavi</em> facilitating further mineralization of the system by degrading 2,4-DCP and its dechlorination products. Most intermediate degradation products exhibit lower ecological toxicity than 2,4-DCP, highlighting immobilized microorganism as a promising solution for 2,4-DCP pollution.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106071"},"PeriodicalIF":4.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tanveer Kaur , N. Tejo Prakash , M. Sudhakara Reddy
{"title":"Biotransformation and bioaccumulation of selenium by arbuscular mycorrhizal fungi associated with maize roots in natural seleniferous soils","authors":"Tanveer Kaur , N. Tejo Prakash , M. Sudhakara Reddy","doi":"10.1016/j.ibiod.2025.106068","DOIUrl":"10.1016/j.ibiod.2025.106068","url":null,"abstract":"<div><div>This study evaluated the role of arbuscular mycorrhizal fungi (AMF) isolated from naturally seleniferous soils in promoting plant growth, selenium (Se) uptake, and biotransformation in naturally selenium-contaminated fields. In AMF-inoculated plants, root biomass, shoot biomass, and maize cob biomass increased by 2.35-fold, 2.32-fold, and 1.93-fold, respectively, compared to control plants. Notably, AMF roots accumulated 7.80 % more selenium than control roots. However, selenium accumulation in the shoots and grains of AMF-inoculated plants was reduced by 71.83 % and 49.92 %, respectively. X-ray absorption near-edge spectroscopy revealed that toxic inorganic selenium species present in control plant roots were replaced by reduced elemental selenium in AMF-inoculated plants. Furthermore, AMF-inoculated plants exhibited higher levels of volatile organic selenium compounds, such as dimethyl selenide and dimethyldiselenide, which escaped from plant tissues, acting as a detoxification mechanism to mitigate selenium toxicity. To the best of our knowledge, this study is the first to report the role of indigenous AMF isolated from selenium-contaminated soils in facilitating selenium bioaccumulation and biotransformation to less toxic forms in plants under field conditions. The findings highlight that AMF not only enhance plant growth under selenium-stressed conditions by limiting selenium translocation to above-ground tissues but also protect plants by transforming selenium into methylated volatile derivatives. Thus, AMF protect the plants from Se toxicity by biotransformation to lesser toxic forms as well as enhancing plant growth activities. This approach can lead to successful utilization of seleniferous soils, which otherwise are considered not suitable for agricultural use.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106068"},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biodeterioration of cultural heritage monuments: A review of their deterioration mechanisms and conservation","authors":"Sangeeta Yadav , Diane Purchase","doi":"10.1016/j.ibiod.2025.106066","DOIUrl":"10.1016/j.ibiod.2025.106066","url":null,"abstract":"<div><div>Geochemical cycles result in the chemical, physical, and mineralogical modification of rocks, ultimately leading to the formation of soil. However, when stones and rocks form part of historic buildings and monuments, the effects are deleterious. In addition, microorganisms colonize these monuments over time, resulting in the formation of biofilms, while microbial metabolites cause physical weakening and discoloration of the stone. This process, known as biodeterioration, results in a significant loss of cultural heritage. Purification and 16S rDNA sequencing of bacteria growing on heritage monuments revealed that the most prominent taxa were closely related to <em>Bacillus</em> spp.<em>, Arthrobacter</em> spp., <em>Staphylococcus</em> spp. and <em>Paenibacillus</em> spp. In addition to bacteria, some fungal strains of the genera <em>Penicillium</em>, <em>Aspergillus</em>, <em>Fusarium</em> and <em>Alternaria</em> were also reported. To formulate effective conservation strategies to prevent biodeterioration and restore monuments, it is important to identify the microorganisms colonizing the substrate and the energy sources they utilize for sustenance. With this perspective, this review focuses on studies that explored the process of biodeterioration, the mechanisms by which microbes colonize and impact monuments, the techniques used to assess biodeterioration and conservation strategies designed to preserve the monuments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106066"},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lei Zhou , Jie Gao , Yu-Xuan Li , Jun Wu , Biao Wang , Yi-Fan Liu , Shi-Zhong Yang , Ji-Dong Gu , Bo-Zhong Mu
{"title":"A new control strategy of sulfate-reducing microorganisms and corrosion rate from a high-temperature oil reservoir by nitrate/nitrite coupling with nitrate-reducing bacteria","authors":"Lei Zhou , Jie Gao , Yu-Xuan Li , Jun Wu , Biao Wang , Yi-Fan Liu , Shi-Zhong Yang , Ji-Dong Gu , Bo-Zhong Mu","doi":"10.1016/j.ibiod.2025.106065","DOIUrl":"10.1016/j.ibiod.2025.106065","url":null,"abstract":"<div><div>Different water-flooded oil reservoirs shape diverse compositions of sulfate-reducing microorganisms (SRM), and the activities of key SRM may involve different metabolic, corrosion and inhibition mechanisms. However, the current bioinhibition methods for SRM in various petroleum reservoirs are complex and variable, and universal and systematic model methods are lacking for field application. In the present study, the effective inhibition of SRM from the production water of the Jiangsu Oilfield was investigated using inhibitors and nitrate-reducing bacteria (NRB). The experiments, involving different concentrations of selective inorganic inhibitors, showed that nitrate and nitrite exhibited prominent inhibitory effects on the SRM community (JS) with sulfide reduction by 49.0%–62.5%, followed by molybdate and tungstate. With the target of the key SRM (<em>Archaeoglobus</em>) in the production water of the Jiangsu Oilfield, nitrate/nitrite and three NRB strains were evaluated for their inhibition performance. The nitrate-containing treatments were more related to the NRB species, whereas the nitrite-containing treatments were more related to nitrite concentrations. Comparatively, the sulfide inhibition of the nitrite-containing treatments was more significant, with an obvious influence on enzyme contents (Apr and Dsr). Two preferred inhibition combinations were obtained: nitrate (10 mmol/L) and ZW3; nitrite (5 mmol/L) and ZW3. They have been applied in the SRM community (JS) for effectiveness evaluation, with excellent performance in the control of sulfide production and corrosion rate. A nitrate-mediated model method for the bioinhibition of SRM in oil reservoirs is proposed. These results provide insights and strategies for microbial inhibition of SRM and corrosion control in oilfields.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106065"},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study on the dynamic changes of physicochemical properties, nitrogen functional genes, bacterial communities and their interactions during the composting of Chinese medicinal herbal residues","authors":"Wanting Zheng , Lisheng Wang , Qiannuo Zeng, Jiayi Fu, Minxin Rao, Ying Zhang","doi":"10.1016/j.ibiod.2025.106063","DOIUrl":"10.1016/j.ibiod.2025.106063","url":null,"abstract":"<div><div>Aerobic composting of Chinese medicinal herbal residues (CMHRs) constitutes an efficacious waste management strategy, yet optimizing nitrogen content remains challenging. The variations of physicochemical properties, nitrogen functional genes, and bacterial communities throughout the composting process, along with their interrelationships were explored in this study. The final compost product achieved maturity, with a C/N ratio of 10.96 and a nitrification index of 0.19. Quantitative PCR analysis indicated suboptimal nitrification efficiency due to insufficient activity of nitrifying microorganisms. 52 biomarkers at four stages of composting were identified by linear discriminant analysis effect size. Functional annotation of prokaryotic taxa exposed chemoheterotrophy (17.05%–25.52%) and aerobic chemoheterotrophy (11.84%–22.65%) as dominant bacterial functions. Canonical correspondence analysis discovered total nitrogen exerted the most significant impact on bacterial genus distribution. Bacteria affectting nitrification genes and nitration products, especially <em>Anseongella</em> and <em>Weissella</em>, were spotted through network analysis. The results revealed the important microbial groups facilitating the nitrogen conversion of compost of CMHRs, which provided a basis for optimizing nitrogen retention and improving the quality of compost.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106063"},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hai Nguyen , Hoang Le , Le Nguyen , Anh Do , Oanh Le , Hang Dinh
{"title":"Biodegradation of chicken feathers under oxygen-limited conditions: An alternative approach for nutrient recovery from feather waste","authors":"Hai Nguyen , Hoang Le , Le Nguyen , Anh Do , Oanh Le , Hang Dinh","doi":"10.1016/j.ibiod.2025.106064","DOIUrl":"10.1016/j.ibiod.2025.106064","url":null,"abstract":"<div><div>Poultry feathers are a challenging-to-degrade solid waste that requires proper management and treatment technologies. Conventionally, feather waste is treated by composting, which requires oxygen and takes a long time. Here, we present a method for degrading feather waste under oxygen-limited conditions, shortening the treatment time to 8 weeks while achieving a high decomposition efficiency of 81 %. The emission of sulfide odor was controlled as the degradation process was performed in closed chambers without aeration. The process produced feather lysate at a high yield of 0.9 L kg<sup>−1</sup>, retaining most of the nutrients from the feather waste. No PAHs, heavy metals, or pathogens were detected in the feather lysate, indicating a low risk for agricultural use. Feather lysate with a high keratinase content of 1330.13 U mL<sup>−1</sup> can be a source for obtaining this enzyme for various applications. The bacterial genera <em>Keratinibaculum</em> and <em>Bacillus</em> were identified as key drivers of the feather decomposition process.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106064"},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}