International Biodeterioration & Biodegradation最新文献

{"title":"Optimizing biodegradation of polychlorinated biphenyls (PCBs) using bacteria isolated from common effluent treatment plant (CETP) sludge: Integration of machine learning, kinetic studies, and metabolomic analysis","authors":"Pooja Thathola ,&nbsp;Soumya Haldar","doi":"10.1016/j.ibiod.2024.105992","DOIUrl":"10.1016/j.ibiod.2024.105992","url":null,"abstract":"<div><div>The present study demonstrates the effective biodegradation of six polychlorinated biphenyls (PCBs) using microbial consortia consisting of <em>Bacillus</em> sp. and <em>Stutzerimonas</em> sp. Isolated from common effluent treatment plant (CETP) sludge for sustainable environmental remediation. The process during the degradation was optimized using machine learning Artificial Neural Networks (ANN) and Response Surface Methodology (RSM) with Box-Behnken Design (BBD), leading to high degradation efficiencies: 100% for PCB28 and PCB52, 98.89% for PCB101, 98.85% for PCB138, 95.89% for PCB153, and 88.56% for PCB180. The metabolites generated during the study are less toxic and nontoxic. The consortium followed two degradation pathways; acetyl CoA and Pyruvic acid. Further, the degradation kinetics studied during the process reveals that PCB138,153and 180 followed first-order kinetics, while PCB 28, 52, and 101 followed second-order kinetics. Moreover, the bacterial growth kinetics were studied using the Gompertz fitting curve with an R² value of 0.9405. The findings underscore the importance of fine-tuning biodegradation processes to enhance their efficiency, ultimately contributing to greener, more sustainable methods for PCB remediation. By integrating machine learning and statistical optimization, this study paves the way for future advancements in environmental biotechnology aimed at mitigating the adverse impacts of PCBs on ecosystems and human health.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105992"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of glutaraldehyde and formaldehyde for enhancing decay resistance and reducing caffeine leachability in Scots pine wood","authors":"Yeray Manuel López-Gómez ,&nbsp;Ville H. Nissinen ,&nbsp;Aitor Barbero-López ,&nbsp;Martti Venäläinen ,&nbsp;Antti Haapala","doi":"10.1016/j.ibiod.2025.106014","DOIUrl":"10.1016/j.ibiod.2025.106014","url":null,"abstract":"<div><div>Caffeine-treated wood is valued for its decay resistance, but caffeine high leachability under outdoor conditions significantly reduces its long-term effectiveness. This study aimed to mitigate the leachability of caffeine-impregnated Scots pine wood by incorporating either formaldehyde or glutaraldehyde. The treatments were evaluated using standard leachability analysis (EN 84), decay resistance testing (EN 113), mass spectrometry, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, and mechanical testing. Leaching tests revealed that caffeine-glutaraldehyde-impregnated wood exhibited the lowest mass loss after leaching (3.30 ± 0.15%), outperforming caffeine-treated (6.18 ± 0.15%) and caffeine-formaldehyde-treated (5.94 ± 0.13%) specimens, indicating superior caffeine fixation. ATR-FTIR spectroscopy showed that characteristic caffeine bands diminished following leaching, confirming caffeine leachability. However, caffeine-glutaraldehyde-treated samples exhibited less pronounced spectral changes compared to caffeine-formaldehyde-treated samples. Mass spectrometry further corroborated these findings, detecting higher caffeine content in glutaraldehyde-treated wood than in caffeine-only specimens after leaching test. Decay resistance tests demonstrated that caffeine-glutaraldehyde-treated wood retained high resistance to fungal decay both before and after leaching. Mechanical tests revealed that the modulus of rupture (MOR) was preserved post-leaching only in caffeine-glutaraldehyde-treated samples. These findings highlight the effectiveness of low concentrations of glutaraldehyde in reducing caffeine leachability, thereby enhancing the decay resistance and durability of treated wood, making it a promising approach for outdoor applications.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106014"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MIL-88A(Fe) modified carriers induced iron autotrophic denitrification in intermittently-aerated MBBR for low C/N wastewater treatment","authors":"Zhonghong Guo,&nbsp;Jiaqi Yang,&nbsp;Ya-nan Luan,&nbsp;Yue Yin,&nbsp;Feng Zhang,&nbsp;Changqing Liu","doi":"10.1016/j.ibiod.2025.106009","DOIUrl":"10.1016/j.ibiod.2025.106009","url":null,"abstract":"<div><div>Iron-assisted wastewater treatment has garnered significant attentions due to its promising potential for nitrogen removal under low carbon/nitrogen (C/N) ratios. However, the continuous addition of iron would result in unacceptably high operational cost. Herein, the iron mental-organic frame (MIL-88A(Fe)) modified sponge (MS) was developed as a source of continuous reactive iron sites and integrated in an intermittently-aerated moving bed biofilm reactor (MBBR) to enhance nitrogen removal efficiency under low (C/N) ratios. The results demonstrated that the MS-assisted system achieved stable total nitrogen removal of 76.2 ± 6.0% without adding additional iron and carbon sources when the C/N was 3.5 ± 0.3. After MS addition, the electron transport system activity increased from 8.2 ± 0.9 μgO₂/g/h to 12.7 ± 2.6 μgO₂/g/h, and the scanning electron microscope images revealed that the MS were covered with biofilm. Meanwhile, iron autotrophic bacteria (<em>Thermomonas</em>, <em>Dechloromonas</em>) and heterotrophic denitrifying bacteria (<em>Candidatus_Competibacter</em>) were enriched in the MS-assisted system, confirming the successful inducing of iron autotrophic denitrification process inside the reactor. This study revealed the effect of MS addition on microbial community succession and their pollutants removal characteristics, offering a feasible strategy for low C/N wastewater treatment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106009"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro-Phyto-assisted reduction of Hexachlorocyclohexane (HCH) contamination in soil: A sustainable solution to mitigate HCH pollution","authors":"Aditi Roy ,&nbsp;Priya Dubey ,&nbsp;Sandhya Mishra ,&nbsp;Poornima Vajpayee ,&nbsp;Suchi Srivastava ,&nbsp;Pankaj Kumar Srivastava","doi":"10.1016/j.ibiod.2024.105974","DOIUrl":"10.1016/j.ibiod.2024.105974","url":null,"abstract":"<div><div>This study pioneers a sustainable solution for Hexachlorocyclohexane (HCH) remediation through a unique microbe-assisted phytoremediation strategy, utilizing <em>Ricinus communis</em> inoculated with a novel yeast-bacteria rhizo-inoculum, <em>Priestia megaterium</em> and <em>Meyerozyma caribbica</em>. This combination achieved unprecedented reductions of its various isomeric residues <em>viz</em>. α-HCH (85.09%), β-HCH (91.80%), γ-HCH (95.24%), and δ-HCH (89.78%) over a 90-day pot experiment. <em>R. communis</em> demonstrated significant bioaccumulation of ΣHCH, primarily in its roots, followed by shoots and leaves. The HCH-exposed plants treated with mixed culture treatment exhibited a marked decrease in antioxidative defense enzyme activities: SOD (79%), APX (56%), GPX (68%), and CAT (55%) compared to control plants. Additionally, reduction of HCH residues in rhizospheric soil was significantly enhanced in the presence of microbial inoculum. Multivariate Principal Component Analysis (PCA) indicated significant differences in soil physico-chemical properties and plant enzymatic activities between treatments, closely linked to variations in HCH concentrations. PCA components accounted for 49.07% (PC1) and 15.02% (PC2) of the total variance in soil properties, and 65.66% (PC1) and 19.04% (PC2) in root enzymatic activities, with leaf enzymatic activities showing 69.70% (PC1) and 15.20% (PC2). These results suggest that inoculating <em>R. communis</em> with this novel yeast-bacteria mixed culture offers a highly effective and promising approach for the remediation of HCH-contaminated sites.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105974"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the compost-ability of oil refinery sludge in the presence or absence of earthworms: An eco-friendly approach of biodegradation","authors":"Snigdha Nath ,&nbsp;Ujjal Dutta ,&nbsp;Sumpam Tangjang ,&nbsp;Subhasish Das","doi":"10.1016/j.ibiod.2024.105987","DOIUrl":"10.1016/j.ibiod.2024.105987","url":null,"abstract":"<div><div>Composting offers a low-cost and sustainable approach in waste management, however, knowledge about its feasibility in oil refinery sludge (ORS) bioconversion is unknown. We investigated the efficiency of three composting systems, viz., <em>Eisenia</em>-system (with <em>Eisenia fetida</em>), <em>Eudrilus</em>-system (with <em>Eudrilus eugeniae</em>), and non-earthworm system for ORS bioconversion. The earthworm-based systems recorded greater increment in available nutrients and reduction of heavy metals than non-earthworm system. The <em>Eudrilus</em>-system recorded an increment in total bacterial count by 10%, while P-solubilizing bacteria increased five-folds in <em>Eisenia</em>-system. Additionally, higher economic benefit was observed in terms of net present value (US $ 203.08), benefit-to-cost ratio (0.63) and pay-back time (1 year and 6 months) in the <em>Eudrilus-</em>processed ORS composting approach. The earthworm-based systems also demonstrated higher activity of urease, alkaline phosphatase and dehydrogenase. The clean index (&gt;4) and fertility index (&gt;3.1) of earthworm-sourced composts reflected their high manurial potential. The compost quality index (CQI &gt;7) indicated that presence of earthworms greatly facilitated the bioconversion of ORS due to higher humification and microbial diversity. Principal component analysis identified water holding capacity, total C, NPK, and earthworm growth as the major contributors in CQI. Moreover, the strong influence of enzymes in CQI (R² = 0.64) was corroborated by the linear regression analysis. Our findings underscore the potential of earthworm-based bio-systems in ORS-remediation, offering a viable way in its management while producing fortified organic manure.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105987"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethiprole biodegradation by Pseudomonas sp. NC1: Insights into the mechanisms and pathways","authors":"Wenjie Wei,&nbsp;Yingying Wu,&nbsp;Zekun Sha,&nbsp;Zhiqiang Lu,&nbsp;Minghua Wang","doi":"10.1016/j.ibiod.2024.105985","DOIUrl":"10.1016/j.ibiod.2024.105985","url":null,"abstract":"<div><div>Ethiprole is a widely used phenylpyrazole pesticide; however, microorganism-mediated degradation of ethiprole has not been reported. In this study, we isolated and identified a new and efficient strain, NC1, of <em>Pseudomonas putida</em>, using morphological, physiological, biochemical, and molecular methods. The strain, identified as <em>Pseudomonas putida</em>, utilizes ethiprole as a carbon source. We optimized the conditions for ethiprole degradation mediated by strain NC1 using the response surface method. Under optimal conditions (25 °C, pH 9, and 0.5% inoculation) we achieved a degradation rate of 79.7% within 24 h for a 50 mg/L ethiprole solution. A new ethiprole degradation pathway is proposed based on the main degradation products. The key oxidoreductase from the glucose–methanol–choline family, GmcF, involved in ethiprole degradation was identified through molecular cloning, and the degradation of other phenylimidazole insecticides by the new strain was verified using molecular docking. Additionally, we elucidated the mechanism underlying NC1 strain-mediated ethiprole degradation. We also examined the potential of the NC1 strain for the bioremediation of ethiprole-contaminated lettuce plants and soil. By optimizing this method, strain NC1 degraded 12.1% of 30 mg/L ethiprole in soil over 7 days. The half-life of ethiprole in treated lettuce plants with strain NC1 was reduced by 37.7% compared to the control group, indicating a significant effect of exogenous microorganisms on the elimination of ethiprole in lettuce plants. This study offers significant insights into the degradation pathways and mechanisms of ethiprole biodegradation and provided the basis for bioremediation of ethiprole.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105985"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular docking simulation of anaerobic biodegradation pathways of petroleum hydrocarbons in oil reservoir","authors":"Shiqi Wang ,&nbsp;Qichao Xie ,&nbsp;Haitong Yang ,&nbsp;Xiaodong Chen ,&nbsp;Wen Liu ,&nbsp;Congcong Li ,&nbsp;Shuoliang Wang","doi":"10.1016/j.ibiod.2024.105972","DOIUrl":"10.1016/j.ibiod.2024.105972","url":null,"abstract":"<div><div>In-depth study of the anaerobic biodegradation mechanism of petroleum hydrocarbons is of great significance for microbial oil recovery and bioremediation. This study retrieved microorganisms with genes for anaerobic hydrocarbon degradation from the PATRIC and NCBI databases, and compiled taxonomic information of core microorganisms containing multiple functional genes. Molecular docking simulations were conducted on 11 enzymes involved in the initial degradation pathways, such as fumarate addition reaction, anaerobic hydroxylation, and anaerobic carboxylation. The simulations revealed the petroleum hydrocarbon binding energy, binding affinity, and binding site within the enzyme cavity. The results indicated that the core microbial bacterial lineages with anaerobic degradation functional genes mainly included Bacillota and Proteobacteria. Enzymes in the same initial degradation pathway continuously catalysed the anaerobic degradation of petroleum hydrocarbons through synergistic action. The interaction force between catalytic enzymes and petroleum hydrocarbons was mainly hydrophobic interaction, with some π-π stacking interaction. This study conducted the first molecular simulation of anaerobic degradation of petroleum hydrocarbons, illustrating the interaction mode between petroleum hydrocarbons and enzymes, as well as the anaerobic degradation mechanism of petroleum hydrocarbons.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105972"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of benzene, toluene, ethylbenzene, xylene(s) biodegradation under anoxic conditions using response surface methodology","authors":"Gurpreet Kaur ,&nbsp;Satyam Verma ,&nbsp;Magdalena Krol ,&nbsp;Satinder Kaur Brar","doi":"10.1016/j.ibiod.2024.105973","DOIUrl":"10.1016/j.ibiod.2024.105973","url":null,"abstract":"<div><div>The biodegradation potential and metabolism of bacteria depend on the terminal electron acceptors present at contaminated sites. Due to the quick consumption of oxygen, microorganisms tend to use substitute electron acceptors such as nitrate, sulfate, manganese, and iron for biodegradation. The present study aims to investigate the effect of electron acceptors (nitrate, sulfate, and ferric ions) on BTEX biodegradation using <em>Bacillus infantis (B. infantis)</em> and <em>Microbacterium esteraromaticum (M. esteraromaticum)</em>. The experiment was designed with response surface methodology using the Box-Behnken method. All four compounds of BTEX biodegraded with removal efficiencies ranging from 46% to 57% in Bacillus-treated samples, while 88–98% biodegradation in Microbacterium-treated cultures. The optimal growth of <em>B. infantis</em> was observed at 250 mg/L of nitrate and iron, while no effect of sulfate was observed. For <em>M. esteraromaticum</em>, 250 mg/L of nitrate and sulfate showed the maximum growth of more than 1 optical density (OD), however, no change in growth was noticed with iron treatment. The investigation showed a maximum BTEX biodegradation of 57% by <em>B. infantis</em> under sulfate reduction and overall, 98% by <em>M. esteraromaticum</em> in combined nitrate and sulfate reduction. The present work provides new insights into soil microbial community responses to electron acceptors under anoxic conditions, signifying that intrinsic microorganisms could be successfully stimulated for ISB with electron acceptors as a supplement.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105973"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Niche differentiation of denitrifying anaerobic methane oxidation bacteria and archaea in the permafrost peatlands","authors":"Lingyu Fu ,&nbsp;Xiangwen Wu ,&nbsp;Dalong Ma ,&nbsp;Weiping Yin ,&nbsp;Anwen Liu ,&nbsp;Xu Wang","doi":"10.1016/j.ibiod.2024.105990","DOIUrl":"10.1016/j.ibiod.2024.105990","url":null,"abstract":"<div><div>The anaerobic oxidation of methane (AOM) coupled to either nitrite or nitrate reduction (nitrite or nitrate-DAMO) is a process connecting global nitrogen and carbon cycles. Permafrost peatlands are important natural sources of methane, and climate warming is accelerating permafrost thaw, resulting in changes in water table and vegetation communities that are dramatically reshaping microbial-mediated methane oxidation processes, potentially creating strong positive peatland-climate feedbacks, while the ecology of DAMO bacteria and archaea in peatland soils is poorly understood. Herein, the diversity, abundance, phylogeny, and potential activity of DAMO bacteria and archaea were explored using molecular techniques and stable isotope tracing in three typical peatlands of the Greater Khingan Mountains permafrost regions. The results revealed the co-existence of DAMO bacteria and archaea, with notable variations in community structures across different peatlands, while the vertical distribution within soil profiles remained relatively stable. These variations were mainly affected by factors such as water content, total organic carbon, nitrite, and nitrate in soil. The potential activity and abundance suggested that DAMO bacteria were predominantly found in the middle soil layers, whereas DAMO archaea were more abundant in the bottom layers. Furthermore, the diversity, potential activity, and abundance of DAMO bacteria generally declined along the forest-peatland ecotone, whereas DAMO archaea exhibited an increasing trend. Partial least squares path modeling (PLS-PM) and correlation analyses revealed strong associations between DAMO activities and the abundances of the <em>pmoA</em> and <em>mcrA</em> genes, in addition to substrate availability. The relative contribution of nitrite-DAMO to the total CH₄ oxidation was 16.77%, slightly higher than that of nitrate-DAMO at 13.23%, with both contributing more significantly than AOM coupled to iron oxide reduction (Fe-AOM) at 8.65%, demonstrating that AOM are important processes for mitigating CH₄ emissions in peatlands. This research contributes to a better understanding of the biogeochemical cycling of CH₄ in permafrost peatlands and broaden our insight into the environmental significance of DAMO microorganisms.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105990"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into mechanism of 1-hydroxy-2-naphthoic acid accumulation during phenanthrene degradation by Stutzerimonas frequens SL-6","authors":"Liwei Guo ,&nbsp;Xinyu Zhang ,&nbsp;Qiang Hou ,&nbsp;Hailei Wei ,&nbsp;Yilin Gu ,&nbsp;Baisuo Zhao ,&nbsp;Haisheng Wang","doi":"10.1016/j.ibiod.2024.105975","DOIUrl":"10.1016/j.ibiod.2024.105975","url":null,"abstract":"<div><div>Oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) represent intermediate metabolites accumulating during the degradation of polycyclic aromatic hydrocarbons (PAHs), posing challenges to effective remediation of PAH contamination. Notably, 1-hydroxy-2-naphthoic acid (1H2N), distinguished by its heightened toxicity, emerges as a pivotal model compound for investigating oxy-PAHs. This research endeavors to unravel the mechanism behind the accumulation of 1H2N by <em>Stutzerimonas frequens</em> SL-6, an efficient phenanthrene-degrading bacterium. Genomic analysis of <em>S. frequens</em> SL-6 implicated <em>nahA, salA,</em> and <em>nahR</em> in the accumulation process of 1H2N. This hypothesis was validated through <em>in vitro</em> assessments of enzyme catalytic activity, reinforced by <em>in vivo</em> gene knockout and complementation studies. Our findings pinpoint SalA as a critical factor in the accumulation of 1H2N. Subsequently, the overexpression of SalA in <em>S. frequens</em> SL-6 led to a 70% reduction in the accumulation of 1H2N. These insights deepen the global understanding of microbial PAHs degradation processes and provide a theoretical foundation for the development of microbial strains capable of efficiently and thoroughly degrading PAHs.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105975"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}