International Biodeterioration & Biodegradation最新文献_第7页

Immobilized consortium of heterotrophic nitrifying-aerobic denitrifying bacteria on various matrices for nitrogen removal from synthetic wastewater 在各种基质上固定化异养硝化-好氧反硝化菌群，以去除合成废水中的氮

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-20 DOI: 10.1016/j.ibiod.2024.105888

Noushan Hatami, Zahra Etemadifar, Rasoul Shafiei

{"title":"Immobilized consortium of heterotrophic nitrifying-aerobic denitrifying bacteria on various matrices for nitrogen removal from synthetic wastewater","authors":"Noushan Hatami, Zahra Etemadifar, Rasoul Shafiei","doi":"10.1016/j.ibiod.2024.105888","DOIUrl":"10.1016/j.ibiod.2024.105888","url":null,"abstract":"<div>Environmental contamination by nitrogen compounds such as ammonium and nitrate has increased extensively in the recent past, which necessitates the development of eco-friendly remediation technologies. In this study, three matrix types including pumice, aquarium ceramic filter, and calcium alginate beads were used to facilitate nitrogen removal with an immobilized heterotrophic nitrifying-aerobic denitrifying (HNAD) bacterial consortium. The HNAD bacterial consortium was made of Pseudomonas monteilii Nht, Pseudomonas mendocina AquaN, Rhodococcus erythropolis R1, and Acinetobacter calcoaceticus SCC2. The quality parameters for immobilization, such as the number of immobilized cells and their viability, were assessed. The highest number of bacterial cells (3.4 × 10 9) was immobilized on the aquarium ceramic filter, with 53% cell viability at 30°Ⅽ for two months. Pumice, aquarium ceramic filter, and calcium alginate achieved NH4+-N removal efficiencies of 85.3 ± 1.7%, 87.3 ± 2.2%, and 77.5 ± 3.99% within 24 h, respectively, and removed NO3−-N by 88.23 ± 0.36%, 93.95 ± 0.00%, and 71.29 ± 6.49% over 60 h. Additionally, immobilized cells on pumice and ceramic filter retained up to 84% of NH4+-N removal efficiency after 14 reuse cycles. These findings indicate that the immobilized HNAD bacterial consortium on the aquarium ceramic filter can be used as a suitable biofilter for treatment of high nitrogen wastewater.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"195 ","pages":"Article 105888"},"PeriodicalIF":4.1,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142011608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-based plastics, biodegradable plastics, and compostable plastics: biodegradation mechanism, biodegradability standards and environmental stratagem 生物基塑料、生物降解塑料和可堆肥塑料：生物降解机理、生物降解性标准和环境策略

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-20 DOI: 10.1016/j.ibiod.2024.105887

Sabzoi Nizamuddin , Abdul Jabbar Baloch , Chengrong Chen , Muhammad Arif , Nabisab Mujawar Mubarak

{"title":"Bio-based plastics, biodegradable plastics, and compostable plastics: biodegradation mechanism, biodegradability standards and environmental stratagem","authors":"Sabzoi Nizamuddin , Abdul Jabbar Baloch , Chengrong Chen , Muhammad Arif , Nabisab Mujawar Mubarak","doi":"10.1016/j.ibiod.2024.105887","DOIUrl":"10.1016/j.ibiod.2024.105887","url":null,"abstract":"<div>Conventional polymers are environmentally damaging materials; therefore, global efforts are being made to gradually replace these conventional polymers with bio-based, biodegradable, and compostable plastics due to claims of being more sustainable than petroleum-based plastics. However, such claims may not be based on reality, and unregulated bio plastics may cause environmental anarchy similar to conventional plastics. The degradation of bioplastics has received significant attention because it is the parameter used to evaluate their end-of-life disposal and to assess their environmental shortcomings - where the bioplastics which degrade completely in different environments, thus, considered as an environmental-friendly polymers. Upon disposal, the bioplastics decompose in a bio-active medium by microorganisms such as algae, bacteria, and fungi or to humus, water, and CO2 by marine water. Different standardization and certification bodies have set the standards for bioplastics, compostable, and biodegradable plastics to evaluate the environmental constraints of bioplastics. These standards support various industries in creating bioplastics. Thus, it is important to harness the regulatory power to bring all the standardization and certification bodies (both at the national and international levels) together in setting standards with a high threshold to classify bio-based plastics, biodegradable plastics, and compostable plastics.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"195 ","pages":"Article 105887"},"PeriodicalIF":4.1,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0964830524001586/pdfft?md5=092b3da2f0958ec4d985b1b37e57a73b&pid=1-s2.0-S0964830524001586-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142011606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the deterioration of mortar by enteromorpha-diatoms during green tide 绿潮期间肠藻硅藻对灰泥的劣化研究

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-20 DOI: 10.1016/j.ibiod.2024.105885

Xiaojie Chen , Hui Rong , Dee Liu , Yang Feng , Zhihua Liu , Yong Yang

{"title":"Study on the deterioration of mortar by enteromorpha-diatoms during green tide","authors":"Xiaojie Chen , Hui Rong , Dee Liu , Yang Feng , Zhihua Liu , Yong Yang","doi":"10.1016/j.ibiod.2024.105885","DOIUrl":"10.1016/j.ibiod.2024.105885","url":null,"abstract":"<div>Similar to the deterioration of construction materials by airborne algae, hydrophytic algae can deteriorate cementitious materials and change the performance of structures, thereby reducing the safety and economics of marine facilities. Frequent outbreaks of green tides have occurred in recent years, and algae have been shown to deteriorate calcium substances in cementitious materials. Therefore, the pattern of deterioration of cement by algae as a result of green tide outbreaks is important for extending the durability of marine installations. For this reason, the dominant species of the green tide outbreak, Enteromorpha, and the marine primary producer, diatoms, were selected to simulate the green tide outbreak in this paper. Then, in the paper, the colonization law of Enteromorpha and diatom and the calcium loss law in mortar are discussed. Results showed four stages of Enteromorpha-diatoms colonization on the mortar surface (diatoms colonization within 15 days, release of spores from Enteromorpha, germination of spores from Enteromorpha within 60 days, and transformation of the relationship between the colonization of Enteromorpha and diatoms). Three channels for the loss of calcium on the surface of the mortar include the uptake and transformation, the complexation of biofilm, and the dissolution and deposition. Additionally, Enteromorpha-diatoms can deteriorate the mortar surface, mineral composition, and microstructure.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"195 ","pages":"Article 105885"},"PeriodicalIF":4.1,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142011607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel lactobacillus consortium for effective zearalenone adsorption and biodegradation 有效吸附和生物降解玉米赤霉烯酮的新型乳酸菌群

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-19 DOI: 10.1016/j.ibiod.2024.105889

Bilal Murtaza , Lili Wang , Xiaoyu Li , Ashiq Ali , Shahbaz Ul Haq , Li Ji-bin , Yongping Xu

{"title":"Novel lactobacillus consortium for effective zearalenone adsorption and biodegradation","authors":"Bilal Murtaza , Lili Wang , Xiaoyu Li , Ashiq Ali , Shahbaz Ul Haq , Li Ji-bin , Yongping Xu","doi":"10.1016/j.ibiod.2024.105889","DOIUrl":"10.1016/j.ibiod.2024.105889","url":null,"abstract":"<div>The Lactobacillus consortium LBC-4 was evaluated for its ability to mitigate zearalenone (ZEN) contamination, addressing a critical food and health security issue. This study highlights the potential of LBC-4 in mycotoxin detoxification through comprehensive morphological and genotypic analyses. The LBC-4 consortium, confirmed by PCR to encompass various Lactobacillus strains, efficiently adsorbed 88.7% and 89% of ZEN within 1 and 24 h at 37 °C, respectively, demonstrating a dynamic relationship between bacterial growth and mycotoxin sequestration. Optimal ZEN removal was achieved at 37 °C and pH 7.0 to 8.0. The mechanisms of ZEN removal were elucidated, revealing the roles of cell wall, viable cells, and culture supernatants. Heat-treated LBC-4 showed enhanced ZEN adsorption, supported by Scanning Electron Microscopy and Fourier Transform Infrared Analysis, which indicated significant structural changes. Additionally, LBC-4 degraded ZEN into less toxic derivatives, including 6, 8, 10-trihydroxy zearalenol-14-sulfate, 6, 8, 10-trihydroxy zearalenol, 6, 8-dihydroxy zearalenol, and 6, 8, 10-trihydroxy zearalenol, underscoring the consortium's multifaceted mechanisms and its potential for effective microbial ZEN removal.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"194 ","pages":"Article 105889"},"PeriodicalIF":4.1,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142006708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electric field effect of microbial fuel cells on biological reactions: A review 微生物燃料电池对生物反应的电场效应：综述

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-19 DOI: 10.1016/j.ibiod.2024.105886

Shan Huang , Jingran Zhang , Huimin Zhang , Chuqiao Wang , Chenglong Zou , Yiran Zhang , Guangcan Zhu

引用次数: 0

Conservation of stone heritage buildings: Exploring the algicidal properties of biologically-synthesized nano-silver 保护石质文物建筑：探索生物合成纳米银的杀藻特性

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-17 DOI: 10.1016/j.ibiod.2024.105878

Mingzhong Long , Zhiyi Xu , Yingqiu Chen , Kehua Wu , Shengyu Yang

{"title":"Conservation of stone heritage buildings: Exploring the algicidal properties of biologically-synthesized nano-silver","authors":"Mingzhong Long , Zhiyi Xu , Yingqiu Chen , Kehua Wu , Shengyu Yang","doi":"10.1016/j.ibiod.2024.105878","DOIUrl":"10.1016/j.ibiod.2024.105878","url":null,"abstract":"<div>The degradation of stone heritage buildings by pioneer plants such as algae is a major challenge in heritage conservation research worldwide. At present, there is a lack of effective algaecides to inhibit the growth of algae on stone structures, prompting an urgent need to explore effective green methods to remove algae colonizing on surfaces of stone heritage buildings. Therefore, we explored the use of aqueous and alcoholic extracts of four plant leaves (Cinnamomum cassia leaf, Syzygium aromaticum leaf, Thymus mongolicus leaf and Pogostemon cablin leaf) to prepare silver nanoparticles. By optimizing the synthetic parameters, we optimized the yield of the nanoparticles and examined their algicidal activities through in-vitro and in-situ experiments. The experimental results showed that the chlorophyll-a concentrations of algae treated with nano-silver synthesized from the alcoholic extract of Cinnamomum cassia leaf decreased approximately threefold, and the algae removal rate reached 71.34%. Meanwhile, nano-silver treatment made the color of the stone chips colonized with algae close to the color of the uncolonized algae, which demonstrated that the nano-silver can effectively remove the algae colonized on the stone chips. This study confirms that nano-silver synthesized from plant leaves offer a viable strategy as a ‘green’ algaecide for stone heritage buildings.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"194 ","pages":"Article 105878"},"PeriodicalIF":4.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141998017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Versatile applications and mechanisms of genus Exiguobacterium in bioremediating heavy metals and organic pollutants: A review Exiguobacterium 属在重金属和有机污染物生物修复中的广泛应用和机制：综述

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-17 DOI: 10.1016/j.ibiod.2024.105884

Min Xiao, Zhong-er Long, Xueqin Fu, Long Zou

{"title":"Versatile applications and mechanisms of genus Exiguobacterium in bioremediating heavy metals and organic pollutants: A review","authors":"Min Xiao, Zhong-er Long, Xueqin Fu, Long Zou","doi":"10.1016/j.ibiod.2024.105884","DOIUrl":"10.1016/j.ibiod.2024.105884","url":null,"abstract":"<div>Microbial remediation presents a promising and sustainable approach for combating environmental pollutants. The genus Exiguobacterium thrives in diverse habitats, including extreme environments, and effectively mitigates a wide array of pollutants through its versatile detoxification mechanisms. Notably, these bacteria are adept at removing heavy metals such as chromium, arsenic, cadmium, mercury, lead, nickel, and vanadium from both soil and water, thereby reducing their toxicity and bioavailability. Additionally, Exiguobacterium demonstrates significant capabilities in biodegrading various organic pollutants, including synthetic dyes, nitroaromatic compounds, petroleum hydrocarbons, and plastic polymers. Despite its ecological and environmental importance, article dedicated to this genus remains relatively sparse. This review aims to comprehensively summarize the application and mechanism of genus Exiguobacterium in remediating toxic heavy metals and organic pollutants. It begins with a brief description of the genus's taxonomic characteristics and ecological diversity, followed by a detailed examination of its detoxification and biodegradation mechanisms in response to pollutant stress. Furthermore, it proposes avenues for future research, including the discovery of novel functional strains, elucidation of detoxification and degradation pathways, and development of genetic editing tools to enhance practical applications in environmental remediation.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"194 ","pages":"Article 105884"},"PeriodicalIF":4.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141998007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Chinese herbal plants on greenhouse gas emissions from pig manure composting and fermentation 中草药植物对猪粪堆肥发酵过程中温室气体排放的影响

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-17 DOI: 10.1016/j.ibiod.2024.105882

Ya-jun Duan , Hong-sheng Wu , Guang-yao Chen , Feng-yi Hu , Yan-hui Li , Zhen-wei Li , Jin-he Chi , Dong-ming Lu , Xiao-ming Gu , Ming-chun Di

{"title":"Influence of Chinese herbal plants on greenhouse gas emissions from pig manure composting and fermentation","authors":"Ya-jun Duan , Hong-sheng Wu , Guang-yao Chen , Feng-yi Hu , Yan-hui Li , Zhen-wei Li , Jin-he Chi , Dong-ming Lu , Xiao-ming Gu , Ming-chun Di","doi":"10.1016/j.ibiod.2024.105882","DOIUrl":"10.1016/j.ibiod.2024.105882","url":null,"abstract":"<div>To reduce greenhouse gas emissions during the composting of animal manure and promote the resource utilization of Chinese herbal plants, experiments on pig manure composting were conducted in a plastic greenhouse. A mixture of herbal powders (Radix isatidis and Radix Polygoni Multiflori, mixed in a 1:1 ratio) was added to the pig manure at concentrations of 0.05%, 0.1%, and 0.5% (w/w), respectively. The results showed that the emission peaks of greenhouse gases occurred in the early stages of composting. The addition of 0.5% of a mixture of Chinese herbal plant powders resulted in a significant reduction in cumulative emissions of CO2 (45.0%) and N2O (60.0%) compared to the control. This treatment also resulted in the lowest global warming potential, measured at 91.3 g kg−1, and significantly increased the germination index. The study concluded that adding 0.5% Chinese herbal plant powder to compost effectively mitigated greenhouse gas emissions during the composting process.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"194 ","pages":"Article 105882"},"PeriodicalIF":4.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141998006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solidago canadensis enhances its invasion by modulating prokaryotic communities in the bulk soil 加拿大实心草通过调节大块土壤中的原核生物群落增强入侵能力

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-13 DOI: 10.1016/j.ibiod.2024.105881

Wen-Tao Qiao , Yong-Feng Wang , Xue-Yan Hou , Dao-Lin Du , Ze-Yu Li , Xin-Yu Wang

{"title":"Solidago canadensis enhances its invasion by modulating prokaryotic communities in the bulk soil","authors":"Wen-Tao Qiao , Yong-Feng Wang , Xue-Yan Hou , Dao-Lin Du , Ze-Yu Li , Xin-Yu Wang","doi":"10.1016/j.ibiod.2024.105881","DOIUrl":"10.1016/j.ibiod.2024.105881","url":null,"abstract":"<div>Several explanations have been proposed for the rapid spread of Solidago canadensis in various environments. Yet, the specific role of soil prokaryotes in this process remains unclear. To understand the prokaryotic role, we conducted a field study in eastern China, where S. canadensis invaded the native plant Humulus scandens. Prokaryotic communities in the soil were studied across three levels of invasion intensity: low, medium, and high (S. canadensis less than 10, about 50, and above 90%, respectively). We found that the S. canadensis invasion decreased the total prokaryotic abundance in the bulk soil (1.61 ± 0.57 × 108 to 5.78 ± 3.65 × 107 copies g−1 DW), but increased the total prokaryotic abundance in S. canadensis (7.72 ± 5.11 × 107 to 1.27 ± 0.71 × 108 copies g−1 DW) and H. scandens rhizosphere (1.11 ± 0.28 × 108 to 1.79 ± 0.68 × 108 copies g−1 DW). S. canadensis invasion enhanced nutrient-releasing microorganisms (Actinobacteria) (p < 0.05) and disease-resistant microorganisms (Nocardioides) (p < 0.05), while decreasing N-cycling microorganisms (Thaumarchaeota and Nitrospirae) (p < 0.05). This study suggests that S. canadensis may enhance its invasion by modulating the species and relative abundance of functional microorganisms in the bulk soil.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"194 ","pages":"Article 105881"},"PeriodicalIF":4.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The degradation of marine abundant compatible solute dimethylsulfoniopropionate was controlled by TetR-family transcriptional regulator DdaR in Alcaligenes faecalis TetR家族转录调节因子DdaR控制粪钙铝酸盐降解海洋丰富的相容性溶质二甲基磺酰基丙酸盐

IF 4.1 2区环境科学与生态学

International Biodeterioration & Biodegradation Pub Date : 2024-08-08 DOI: 10.1016/j.ibiod.2024.105879

Siqiong Xu , Yongchuang Liu , Yujie Ouyang , Jialiang Li , Gongyi Song , Xiaohui Wang , Pan Yang , Yuehui Tang , Lili Li , Jian He , Jiguo Qiu , Cuiwei Chu , Keshi Ma

{"title":"The degradation of marine abundant compatible solute dimethylsulfoniopropionate was controlled by TetR-family transcriptional regulator DdaR in Alcaligenes faecalis","authors":"Siqiong Xu , Yongchuang Liu , Yujie Ouyang , Jialiang Li , Gongyi Song , Xiaohui Wang , Pan Yang , Yuehui Tang , Lili Li , Jian He , Jiguo Qiu , Cuiwei Chu , Keshi Ma","doi":"10.1016/j.ibiod.2024.105879","DOIUrl":"10.1016/j.ibiod.2024.105879","url":null,"abstract":"<div>The copious compatible solute dimethylsulfoniopropionate (DMSP) plays significant roles in marine ecosystems. The DMSP degradation pathways in strain Alcaligenes faecalis M3A have been comprehensively studied, in which DMSP was cleaved into dimethyl sulphide (DMS) and acrylate. However, the transcriptional regulatory mechanism of DMSP degradation is not fully elucidated. In this study, the TetR/AcrR family transcriptional regulator DdaR repressing acuI operon in strain M3A was investigated. The transcription start sites and promoters of the acuI and ddaR operons was identified. DdaR bound to both the acuI and ddaR promoter regions in EMSA experiment. Two binding sites of DdaR shared conserved motif 5′-CNNCGTNACGNNG-3′ which was essential for the DdaR binding. DdaR was inhibited from binding to the acuI promoter region by acrylate, which acted as a ligand of DdaR. Site-directed mutagenesis was used to investigate the impact of four key amino acid residues (Y61, K67, E135, and I169) in DdaR, revealing their essential roles in the functioning of DdaR. The findings of this study unveil a negative transcriptional regulation mechanism of DMSP degradation in A. faecalis M3A by DdaR and provide a new understanding of the TetR/AcrR-type transcriptional regulators.</div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"194 ","pages":"Article 105879"},"PeriodicalIF":4.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0