International Biodeterioration & Biodegradation最新文献

{"title":"Effects of galU on bacterial physiology and responses to surfactants and antibiotics in Pseudomonas nitroreducens TX1","authors":"Po-Chun Tsai ,&nbsp;Thang Ngoc Tran ,&nbsp;Ting-Huan Shih ,&nbsp;Kyoung Lee ,&nbsp;Chen-Yen Wu ,&nbsp;Shir-Ly Huang","doi":"10.1016/j.ibiod.2025.106072","DOIUrl":"10.1016/j.ibiod.2025.106072","url":null,"abstract":"<div><div><em>Pseudomonas nitroreducens</em> TX1, a Gram-negative bacterium isolated from surfactant-contaminated environments, can utilize octylphenol polyethoxylates (OPEOn) as sole carbon source to grow, making it a promising candidate for bioremediation. One of the transposon insertion mutants exhibited impaired growth on OPEOn was identified as the <em>galU</em> mutant. The <em>galU</em> gene encodes UDP-glucose pyrophosphorylase, which are essential for lipopolysaccharide (LPS) and exopolysaccharide (EPS) biosynthesis. This study investigates the effects of <em>galU</em> in bacterial physiology. The <em>galU</em> deletion mutant exhibited impaired growth on OPEOn, and exhibited higher viability in succinate medium. The mutation also affected cell surface properties, which includes the changes of morphology and cell surface hydrophobicity. Morphological analyses by scanning electron microscopy revealed shortened cells, and altered cell surface roughness of <em>galU</em> mutant. The analyses of cell surface hydrophobicity, revealing the mutant exhibited lower cell surface hydrophobicity during the log phase. The stress resistance of <em>galU</em> mutant were also conducted. Biofilm formation was found to be increased in <em>galU</em> mutant, and antibiotic susceptibility tests showed that this mutant had increased resistance to multiple antibiotics, highlighting the critical role of LPS in maintaining membrane permeability and susceptibility in <em>Pseudomonas</em> species. Complementation of the <em>galU</em> mutation restored the phenotypes in terms of growth, morphology, and cell surface properties. These findings enhanced our understanding of <em>galU</em> by using its in-frame deletion mutant. The effects on bacterial physiology shown the potential for improving biodegradation of nonionic surfactants controlling biofilms and applying on antibiotics development.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106072"},"PeriodicalIF":4.1,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143714882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation of highly toxic reed hydrolysate using Candida glycerinogenes to produce platform compound glycerol","authors":"Yiwen Cheng ,&nbsp;Liya Zhang ,&nbsp;Dongqi Jiang ,&nbsp;Mengying Wang ,&nbsp;Xueqing Du ,&nbsp;Hong Zong ,&nbsp;Xinyao Lu ,&nbsp;Bin Zhuge","doi":"10.1016/j.ibiod.2025.106069","DOIUrl":"10.1016/j.ibiod.2025.106069","url":null,"abstract":"<div><div>Reed, wild plants all over the world, remains underutilized as a fermentation feedstock due to the high inhibitory of its hydrolysate. Most studies on lignocellulose fermentation have focused on ethanol production; however, glycerol, as a platform compound, holds broader application prospects due to its versatility in producing various metabolites. This study aims to enhancing the resistance of <em>Candida glycerinogenes</em> through genetic modifications to improve its resistance to inhibitors. Our results demonstrate that the gene <em>CgLYRM6</em> demonstrated effective detoxification against a range of aldehyde inhibitors, including furfural, vanillin, benzaldehyde, 2,5-dimethylbenzaldehyde, and 3,4-dimethylbenzaldehyde.The final engineered strain Cg1 carrying <em>LYRM6</em>, <em>TAL1</em> and <em>UGA2</em> achieved a glycerol titer of 31 g/L in reed hydrolysate, a 24 % increase compared to the wild type. And glycerol titer reached 40 g/L in the 5 L bioreactor, showing an 60 %increase in production. The strain had shorter fermentation cycle and stronger detoxification ability compared to the control strain. This route enabled simultaneous fermentation and detoxification, providing intermediate metabolites for further conversion and offering a new strategy to utilize undetoxified, alkali-pretreated reed hydrolysate for the production of various metabolites.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106069"},"PeriodicalIF":4.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation and conversion of organics in a continuous HNAD system treating incineration leachate","authors":"Mengying Zhu ,&nbsp;Facai An ,&nbsp;Dezhi Sun ,&nbsp;Xinying Liu","doi":"10.1016/j.ibiod.2025.106073","DOIUrl":"10.1016/j.ibiod.2025.106073","url":null,"abstract":"<div><div>The anaerobically treated incineration leachate contains high-strength ammonium and refractory organics, which is advisable to be biologically treated via heterotrophic nitrification-aerobic denitrification (HNAD) process. However, it is still unknown how the organics in leachate wastewater are degraded and utilized during HNAD process. In this study, a continuous HNAD based moving bed biofilm reactor was operated with actual anaerobically treated incineration leachate as the influent. During stable operation, besides superior nitrogen removal efficiency (88.73 %), the organics were effectively removed with a high average COD removal efficiency (85.05 %). Organic fluorescent spectra showed humic substances were significantly removed, with the intensities of the humic peak and fulvic peak decreasing by 96.59 % and 91.93 %, respectively. Furthermore, most of the dissolved organic matter within varying ranges of molecular weight could be degraded during the HNAD process, during which the benzene-based substances were removed and the medium-long chain alkanes (C &gt; 12) were generated. Through carbon balance analysis, assimilation metabolism accounted for 44.75 % of the conversed organic carbon. According to the decrease in respiration inhibition rate from 52.21 % to 25.36 %, the toxicity of leachate wastewater was strongly reduced after treatment by HNAD process. <em>Halomonas</em> (43.72 %) was the dominant HNAD bacteria, and relative abundances of genes associated with refractory organics degradation were increased during the treatment of incineration leachate. These results provide new insights into carbon metabolism during HNAD process for treating wastewater with complex organics.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106073"},"PeriodicalIF":4.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct responses of aerobic and anaerobic soil microbial community structure and function to anthracene stress at environmental contamination levels","authors":"Liman Wei ,&nbsp;Meng Gao ,&nbsp;Shanxing Wu,&nbsp;Chunguang Liu,&nbsp;Yu Wang,&nbsp;Hongwen Sun","doi":"10.1016/j.ibiod.2025.106074","DOIUrl":"10.1016/j.ibiod.2025.106074","url":null,"abstract":"<div><div>Anthracene (ANT) is widespread in terrestrial ecosystems, yet its impact on soil microbial community structure and function under varying oxygen conditions remains inadequately characterized. This study evaluated the responses of bacteria to ANT stress at environmental concentration (1.0 mg kg⁻¹) under aerobic and anaerobic soils. ANT contamination enriched potential polycyclic aromatic hydrocarbons (PAHs) degrading bacteria, such as Actinobacteriota, Prolixibacteraceae, and Nitrosomonadaceae, under both aerobic and anaerobic conditions. It also resulted in the proliferation of oligotrophic microorganisms under anaerobic condition. Additionally, ANT promoted the functions related to soil carbon, nitrogen and sulfur element cycling functions (Wilcoxon rank sum test, <em>P</em> &lt; 0.05) and altered microbial carbon source utilization preferences in aerobic environment. However, under anaerobic conditions, ANT inhibited soil microbial element cycling, resulting in adverse effects. Furthermore, label-free proteomics quantitative analysis was conducted for aerobic treatment groups, revealing that the ANT contamination in soil altered the growth, metabolism and reproduction of bacteria. Up-regulated proteins were mainly concentrated in the Kyoto Encyclopedia of Genes and Genomes pathways related to amino acid and energy metabolism, which generated energy to satisfy physiological demanding under ANT stress. These results indicate that ANT at environmental contamination levels exhibit varying microbial effects under different oxygen conditions. Therefore, it is crucial to consider both its concentration and soil redox conditions when assessing the risk of PAH compounds.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106074"},"PeriodicalIF":4.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofertilization using microalgae-bacteria consortium for phenolic stress mitigation of sprouted barley: Functional genes profiling of soil bacteria","authors":"Minkee Cho ,&nbsp;Junbeom Jeon ,&nbsp;Jaai Kim ,&nbsp;Sugeun Go ,&nbsp;Heesuk Jung ,&nbsp;Sang-Leen Yun ,&nbsp;Hyokwan Bae","doi":"10.1016/j.ibiod.2025.106070","DOIUrl":"10.1016/j.ibiod.2025.106070","url":null,"abstract":"<div><div>As alternatives to conventional chemical fertilizers, biofertilizers have gained attention, in particular, microalgae-bacteria co-culture biofertilizers (MBCFs) are promising candidates owing to their synergistic interactions. Excessive sludge has been considered a sustainable source of MBCFs due to its proper nutrient composition and biodiversity. This study investigated the feasibility of MBCFs in promoting plant growth. The sprouted barley, selected as a model plant, was exposed to the phenolic stress, and its alleviatory effect by MBCFs was evaluated. The plant's growth characteristics such as germination rate, weight, and length were enhanced after adding MBCFs even under phenol exposure. <em>Desmodesmus</em> and <em>Polaromonas</em> were predominant microalgae and bacteria genus, respectively, and those are attributed to possess the phenol-degrading and plant-promoting properties. The bacterial functional gene prediction revealed several putative genes, including <em>gst</em> (Glutathione R-transferase) and <em>exb</em>B (Iron complex outermembrane recepter protein), which act to promote plant growth. This study suggests that the protective and promotive functions of MBCFs enhance the growth of sprouted barley and alleviate phenolic stress.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106070"},"PeriodicalIF":4.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing alperujo composting strategies for acceleration and quality enhancement","authors":"Evangelina Pareja-Sánchez ,&nbsp;Antonio Jesús García-Moreno ,&nbsp;Melchor Martínez-García ,&nbsp;Lola Pérez-Colodrero ,&nbsp;Laura García-Zapata ,&nbsp;Roberto García-Ruiz","doi":"10.1016/j.ibiod.2025.106076","DOIUrl":"10.1016/j.ibiod.2025.106076","url":null,"abstract":"<div><div>Alperujo (AL), the main byproduct of olive oil production, holds promise for circular economy applications, particularly as a soil amendment. However, composting AL faces challenges, including small-scale facilities and lengthy processes often exceeding four months, increasing costs and limiting capacity. This study evaluated thirteen treatments to accelerate and enhance composting, using AL at seventy percent wet weight mixed with olive leaf as a bulking agent along with various activators and accelerators, including manures, biochar, ash, olive pomace, wood vinegar, and four commercial products with microorganisms. The composting mixture comprised 30 kg wet weight of materials. Temperature, moisture, pH, total organic matter, total nitrogen, phosphorus, potassium, C/N ratio, phytotoxicity, and germination were monitored. Composting duration ranged from twenty-seven to eighty-four days, with chicken manure and ash mixtures achieving the shortest times, between twenty and twenty-seven days. Initial pH and its daily increase were key factors in optimizing composting efficiency, correlating with shorter durations (r between −0.65 and −0.70). The highest-quality composts were obtained with cow, goat, and chicken manure, all surpassing two percent total nitrogen and exhibiting C/N ratios ranging from 14.6 to 26.1. Mean sunflower and pea biomass production and nitrogen use efficiency were between 1.43 to 1.87 times and 1.96 to 4.12 times higher, respectively, for compost enriched with microorganisms, demonstrating their effectiveness in supporting plant development. Composts, except those with ashes, met regulatory limits for heavy metals and polyphenols, confirming their safety and suitability as organic fertilizers. Optimized AL composting mixtures can produce high-quality, eco-friendly amendments, shorten composting times, and improve plant growth.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106076"},"PeriodicalIF":4.1,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic palladium nanoparticles reclaimed by a novel strain Citrobacter braakii Z1 accelerating RDX biodegradation in the munition effluent","authors":"Shichong Zhao,&nbsp;Bin Hou,&nbsp;Kunchan Zhang,&nbsp;Yating Jia,&nbsp;Jing Lu","doi":"10.1016/j.ibiod.2025.106075","DOIUrl":"10.1016/j.ibiod.2025.106075","url":null,"abstract":"<div><div>The microbial degradation of high-energy explosives has emerged as a cost-effective environmental remediation strategy, but is hindered by low degradation efficiency due to the slow electron transfer. Biogenic Pd⁰ nanoparticles (bio-Pd⁰) demonstrate dual functionality, maintaining catalytic activity for N-NO₂ bond reduction while substantially enhancing electron transfer efficiency. This study introduces an innovative single-cell system that synergistically combines chemical catalysis with biological enhancement through in situ synthesis of nanoparticles. Comprehensive characterization through electron microscopy, spectroscopic analysis, and electrochemical measurements revealed that bio-Pd⁰ synthesized by <em>Citrobacter braakii</em> Z1 (<em>C. braakii</em> Z1) were preferentially localized in the periplasmic space and extracellular matrix, forming a stable hybrid named bio-Pd⁰@<em>C. braakii</em> Z1. This process relied on the bio-reduction mediated by biohydrogen and extracellular electron transfer processes via c-type cytochromes (c-Cyts) and flavins. Notably, RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) degradation efficiency increased from 11 % with microbes alone to nearly 100 % with bio-Pd⁰. Although the observed RDX degradation in inactivated bio-Pd⁰@<em>C. braakii</em> Z1 and the detected hydrogenolysis intermediates indicated the participation of chemical catalysis, the negative correlation between RDX degradation rate with the Pd:biomass ratios suggested the biology process dominated RDX degradation. Meanwhile, the higher output current in bio-Pd⁰@<em>C. braakii</em> Z1 and the extracellular electron consumption by RDX indicated the bio-Pd⁰-mediated extracellular biodegradation was the primary driver for improved RDX removal efficiency. Toxicity evaluation confirmed that the in-situ combination of Pd-catalyzed chemical destruction and biological enhancement in a single cell is a feasible and environmentally friendly strategy for RDX removal with a reduced ecological risk.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106075"},"PeriodicalIF":4.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic stress induced by nitrite enhances biocide kill of sulfate reducing bacteria in oilfield enrichments","authors":"Danika Nicoletti ,&nbsp;Bei Yin ,&nbsp;Jordan Schmidt ,&nbsp;Kenneth Wunch ,&nbsp;Lisa Gieg ,&nbsp;Gary Jenneman","doi":"10.1016/j.ibiod.2025.106067","DOIUrl":"10.1016/j.ibiod.2025.106067","url":null,"abstract":"<div><div>Biocides are commonly employed in oilfield settings to mitigate the activity of sulfate reducing bacteria (SRB) that cause biofouling, reservoir souring and microbiologically influenced corrosion. Key challenges of applying biocides in the oilfield include their environmental toxicity and the need to apply high concentrations to overcome their deactivation and degradation by chemical and physical conditions. One strategy to reduce biocide dosages and ecotoxicity is to apply chemicals that enhance their activity. Nitrite, a known metabolic inhibitor of SRB, was previously found to be synergistic in inhibiting sulfate reduction when used in combination with glutaraldehyde. In the present study, pre-treatment of an SRB enrichment with 2.0 mM nitrite to lower cellular ATP was found to enhance the biocidal activity of glutaraldehyde. These results revealed a linear relationship exists between the decrease in adenylate energy charge of an SRB enrichment and a 10,000-fold increase in planktonic SRB kill, supporting a mechanism that metabolic stress imposed by nitrite enhances the biocidal activity of glutaraldehyde. Furthermore, the results showed increasing nitrite pre-treatment times support lower doses of glutaraldehyde needed to achieve enhanced SRB kill while a nitrite concentration of less than 0.04 mM was effective suggesting nitrite is a very efficient biocide enhancer.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106067"},"PeriodicalIF":4.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid removal of 2,4-DCP by activated carbon loaded nano-zero valence iron immobilized microorganisms","authors":"Kangning Dong ,&nbsp;Xiuxia Zhang ,&nbsp;De Li ,&nbsp;Kang Xiong ,&nbsp;Lihan Ren ,&nbsp;Mingbo Sun ,&nbsp;Zhengyong Lv","doi":"10.1016/j.ibiod.2025.106071","DOIUrl":"10.1016/j.ibiod.2025.106071","url":null,"abstract":"<div><div>PEG-nZVI/GAC@<em>B</em> was used to repair 2,4-DCP contamination which was difficult to solve by traditional physical, chemical and biological methods. Characterization techniques confirm the successful incorporation of nZVI particles and <em>Bacillus marisflavi</em> into the GAC's surface folds and pores. The polyethylene glycol 4000 (PEG-4000) coating prevents the formation of Fe³⁺ on nZVI surfaces. Compared to standard GAC, PEG-nZVI/GAC@<em>B</em> demonstrates higher electron transfer efficiency. Key parameters including nZVI content, PEG/nZVI mass ratio, and immobilization time were optimized for maximum efficiency. PEG-nZVI/GAC@<em>B</em> also shows effectiveness in removing other chlorophenol compounds. The removal process primarily involves GAC adsorption, followed by <em>Bacillus marisflavi</em> degradation and nZVI-induced reductive dechlorination. Analyses using IC and GC-MS reveal rapid dechlorination of 2,4-DCP adsorbed on immobilized microorganism by nZVI, with <em>Bacillus marisflavi</em> facilitating further mineralization of the system by degrading 2,4-DCP and its dechlorination products. Most intermediate degradation products exhibit lower ecological toxicity than 2,4-DCP, highlighting immobilized microorganism as a promising solution for 2,4-DCP pollution.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106071"},"PeriodicalIF":4.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotransformation and bioaccumulation of selenium by arbuscular mycorrhizal fungi associated with maize roots in natural seleniferous soils","authors":"Tanveer Kaur ,&nbsp;N. Tejo Prakash ,&nbsp;M. Sudhakara Reddy","doi":"10.1016/j.ibiod.2025.106068","DOIUrl":"10.1016/j.ibiod.2025.106068","url":null,"abstract":"<div><div>This study evaluated the role of arbuscular mycorrhizal fungi (AMF) isolated from naturally seleniferous soils in promoting plant growth, selenium (Se) uptake, and biotransformation in naturally selenium-contaminated fields. In AMF-inoculated plants, root biomass, shoot biomass, and maize cob biomass increased by 2.35-fold, 2.32-fold, and 1.93-fold, respectively, compared to control plants. Notably, AMF roots accumulated 7.80 % more selenium than control roots. However, selenium accumulation in the shoots and grains of AMF-inoculated plants was reduced by 71.83 % and 49.92 %, respectively. X-ray absorption near-edge spectroscopy revealed that toxic inorganic selenium species present in control plant roots were replaced by reduced elemental selenium in AMF-inoculated plants. Furthermore, AMF-inoculated plants exhibited higher levels of volatile organic selenium compounds, such as dimethyl selenide and dimethyldiselenide, which escaped from plant tissues, acting as a detoxification mechanism to mitigate selenium toxicity. To the best of our knowledge, this study is the first to report the role of indigenous AMF isolated from selenium-contaminated soils in facilitating selenium bioaccumulation and biotransformation to less toxic forms in plants under field conditions. The findings highlight that AMF not only enhance plant growth under selenium-stressed conditions by limiting selenium translocation to above-ground tissues but also protect plants by transforming selenium into methylated volatile derivatives. Thus, AMF protect the plants from Se toxicity by biotransformation to lesser toxic forms as well as enhancing plant growth activities. This approach can lead to successful utilization of seleniferous soils, which otherwise are considered not suitable for agricultural use.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"201 ","pages":"Article 106068"},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}