{"title":"Human-derived glutathione S-transferase mu 1 mediated hydrolysis of nitrocellulose: reaction mechanisms and thermal decomposition behavior","authors":"Huihui Xue , Qiaoyu Chen , Juan Huang , Alei Zhang , Yajun Ding , Zhongliang Xiao","doi":"10.1016/j.ibiod.2025.106139","DOIUrl":"10.1016/j.ibiod.2025.106139","url":null,"abstract":"<div><div>This study proposes the innovative application of human-derived glutathione S-transferase mu 1 (GSTM1) for biocatalytic denitration of nitrocellulose (NC), designed to achieve nitrate ester hydrolysis under mild conditions while effectively reducing NC's hazardous properties. The experimental results showed that GSTM1 protein was expressed heterologously in engineered <em>Escherichia coli</em> (<em>E. coli</em>). After 48 h of treatment with purified enzyme GSTM1, the nitrogen content of NC significantly decreased from 12.97 % to 8.4 %. Fourier transform infrared spectroscopic analysis combined with cellulase enzymatic hydrolysis experiments of substances insoluble in ethyl acetate demonstrated GSTM1-mediated conversion of nitrate ester groups to hydroxyl groups in NC, accompanied by hydrogen bond formation and subsequent regeneration of cellulose. Gel permeation chromatography revealed that GSTM1 treatment significantly reduced the relative molecular weight of NC while broadening its molecular weight distribution. Moreover, scanning electron microscopy images revealed increased crack density and surface roughness on NC fiber. The TG-DSC and DSC experiments confirmed decreased combustion heat and reduced activation energy (E<sub>a</sub>) from 195.92 kJ·mol<sup>−1</sup> to 168.47 kJ·mol<sup>−1</sup> post-treatment of NC. The thermal decomposition process of NC before and after the reaction was thoroughly analyzed using the model-free Friedman method. Through systematic optimization, the ideal enzymatic reaction parameters were established as 3.2 g·L<sup>−1</sup> NC substrate concentration with 1.0 g·L<sup>−1</sup> GSTM1 at 37 °C for 8 h. This novel technique safely and environmentally friendly treats waste military compounds while offering new insights for developing multi-enzyme synergistic systems for NC-based waste treatment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106139"},"PeriodicalIF":4.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zizhuo Li , Jinya Wei , Jitao Li , Yaling Yang , Dezhi Yang , Yuzhu Song
{"title":"Ciprofloxacin-based zinc-doped carbon dots with peroxidase-like activity against Escherichia coli and biofilm eradication","authors":"Zizhuo Li , Jinya Wei , Jitao Li , Yaling Yang , Dezhi Yang , Yuzhu Song","doi":"10.1016/j.ibiod.2025.106134","DOIUrl":"10.1016/j.ibiod.2025.106134","url":null,"abstract":"<div><div>Herein, we rationally designed a novel and forceful ciprofloxacin (CIP) grafted zinc-doped-carbon dots (CIP@Zn/CDs) as an efficient artificial nanozyme against <em>Escherichia coli</em> (<em>E. coli</em>), multi-drug-resistant <em>E. coli</em> (AREC), and <em>E. coli</em> biofilms through the peroxidase (POD)-like catalytic activity and the synergistic antibacterial activity of CIP and Zn<sup>2+</sup>. On the one hand, extracellular DNA (eDNA) cleave by high levels of hydroxyl radicals (•OH) catalyzed by the POD-like catalytic activity of CIP@Zn/CDs. On the other hand, CIP@Zn/CDs with positive surface potential, elimination abilities against <em>E. coli</em> via electrostatic interaction, changing the cell membrane permeability and eventually leading to <em>E. coli</em> death within <em>E. coli</em> biofilms. Furthermore, the ultrasmall size of CIP@Zn/CDs possesses good biocompatibility favoring clinical translation. This work provides potential to develop CD-based techniques for applications in the field of selective antibacterial agents towards Gram-negative bacteria.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106134"},"PeriodicalIF":4.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ching Yi Hong , Khalida Muda , Mohamad Ali Fulazzaky
{"title":"Mechanisms and mass transfer kinetics of anammox during sludge enrichment","authors":"Ching Yi Hong , Khalida Muda , Mohamad Ali Fulazzaky","doi":"10.1016/j.ibiod.2025.106140","DOIUrl":"10.1016/j.ibiod.2025.106140","url":null,"abstract":"<div><div>Mass transfer kinetics for enrichment of anammox granular sludge (AnGS) is important for optimizing the removal of ammonium (NH<sub>4</sub><sup>+</sup>) from wastewater. AnGS enrichment was monitored over 147 days in a continuous upflow sludge bed anammox reactor (CUSBAR) experiment, which was simulated using Generalized Fulazzaky equations to predict the underlying mechanisms and mass transfer kinetics. The removal efficiency of NH<sub>4</sub><sup>+</sup> followed the bacterial growth progressed through four distinct phases over 63 days, starting with an initial NH<sub>4</sub><sup>+</sup> concentration of 30.9 mg/L. The CUSBAR process achieved a performance of 84.97 % on the 147th day of the experiment, with the initial NH<sub>4</sub><sup>+</sup> concentration increased from 30.9 to 75.0 mg/L. The internal mass transfer (IMT) and global mass transfer rates were nearly identical and significantly higher than the external mass transfer (EMT) rate, indicating that EMT was the dominant resistance to mass transfer. The positive correlation between NH<sub>4</sub><sup>+</sup> removal efficiency and IMT rate provides valuable insights into the dynamic response of AnGS development during the 147-day enrichment period. This study's findings enhance the availability of AnGS as inocula for future CUSBAR process applications, expanding the knowledge base and references in anammox-based nitrogen removal technology.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106140"},"PeriodicalIF":4.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Loryn L. Bowen , Molly E. Chapleau , Richard R. Chapleau , Oscar N. Ruiz , Thusitha S. Gunasekera
{"title":"Novel bio-recognition elements (BREs) for biosensing and differentiation of Gram-positive bacteria in hydrocarbon environments","authors":"Loryn L. Bowen , Molly E. Chapleau , Richard R. Chapleau , Oscar N. Ruiz , Thusitha S. Gunasekera","doi":"10.1016/j.ibiod.2025.106130","DOIUrl":"10.1016/j.ibiod.2025.106130","url":null,"abstract":"<div><div>Excessive microbial growth in fuel and fuel systems can lead to biofouling and biocorrosion, which makes early detection crucial. A wide range of phylogenetically and metabolically diverse microorganisms have been isolated from environments exposed to hydrocarbons with Gram-positive and Gram-negative bacteria being ubiquitous. The aim of this work was to identify specific biomarker-based bio-recognition elements (BREs) for sensing hydrocarbon-degrading Gram-positive bacteria. We used two bacterial surface targets that are conserved and specific for Gram-positive bacteria, Lipoteichoic acid (LTA) and a short surface-epitope region from the cell wall-associated secreted protein Fasciclin 1 (FASC1), from <em>Gordonia sihwensis</em>. Several peptide-based ligands to detect numerous Gram-positive bacteria in fuel were discovered by using phage display technology and the binding of these peptides to Gram-positive bacteria in the presence of hydrocarbons was validated using Microscale Thermophoresis (MST) and Western blot analysis. The binding peptides were conjugated to quantum dots and their specificity to Gram-positive bacteria was characterized by fluorescence imaging and fluorometric analysis in the presence of target and non-target microorganisms. It was shown that jet fuel did not interfere with binding of the peptides to the target biomarker in the bacteria, indicating that these BREs are suitable for functionalization of biosensors for detection of Gram-positive bacteria in hydrocarbon rich environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"204 ","pages":"Article 106130"},"PeriodicalIF":4.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Sánchez-Arroyo , Laura Plaza-Vinuesa , Blanca de las Rivas , José Miguel Mancheño , Rosario Muñoz
{"title":"Unravelling OTA-detoxification by the dioxin-mineralizing bacterium Rhizorhabdus wittichii RW1T","authors":"Ana Sánchez-Arroyo , Laura Plaza-Vinuesa , Blanca de las Rivas , José Miguel Mancheño , Rosario Muñoz","doi":"10.1016/j.ibiod.2025.106131","DOIUrl":"10.1016/j.ibiod.2025.106131","url":null,"abstract":"<div><div>Environmental pollution remains a critical concern for public health, necessitating innovative remediation strategies. In this regard, microorganisms play a pivotal role by naturally degrading and eliminating various pollutants. The <em>Rhizorhabdus wittichii</em> RW1<sup>T</sup> strain is recognized for its ability to metabolize a broad spectrum of toxic and persistent environmental contaminants. Given the relevance of ochratoxin A (OTA) as a harmful mycotoxin affecting agricultural settings, this study investigates the capability of <em>R. wittichii</em> RW1<sup>T</sup> (DSM 6014<sup>T</sup>) in neutralizing OTA. Experimental results demonstrate that this strain effectively breaks down OTA by hydrolyzing its amide bond, leading to the production of ochratoxin α and L-<em>β</em>-phenylalanine, both of which are non-toxic. Computational genome analysis of <em>R. wittichii</em> RW1<sup>T</sup> revealed genes encoding proteins homologous to known OTA-degrading amidohydrolases. This investigation identified a 438-amino acid protein (ABQ67280.1), whose recombinant form (<em>Rw</em>OTA) efficiently hydrolyzes OTA and ochratoxin B through a catalytic mechanism involving a binuclear metal center. This center facilitates the polarization of the carbonyl group of the scissile amide bond of OTA and activates a water molecule for hydrolysis. Screening against a synthetic peptidase substrate library indicated a highly specific hydrolytic activity for <em>Rw</em>OTA. Structural modeling and molecular docking simulations provided insights into the interaction of ochratoxins with <em>Rw</em>OTA, shedding light on its substrate specificity. The gene encoding <em>Rw</em>OTA presents a promising addition to the extensive repertoire of biodegradation-associated genes within <em>R. wittichii</em> RW1<sup>T</sup>, reinforcing its potential in environmental pollutant detoxification.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106131"},"PeriodicalIF":4.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olga Długosz , Anna Żebracka , Wioletta Wnuk , Agata Drabik , Marta Sochocka , Dominika Franz , Anna Chmielowiec-Korzeniowska , Marcin Banach
{"title":"Bioactivity and antibiofilm activity of metal and non-metal nanoparticles in DES against resistant microorganisms","authors":"Olga Długosz , Anna Żebracka , Wioletta Wnuk , Agata Drabik , Marta Sochocka , Dominika Franz , Anna Chmielowiec-Korzeniowska , Marcin Banach","doi":"10.1016/j.ibiod.2025.106121","DOIUrl":"10.1016/j.ibiod.2025.106121","url":null,"abstract":"<div><div>The increasing resistance of microorganisms to antibiotics necessitates the search for new therapeutic strategies, including the use of nanoparticles with bioactive properties. The aim of this study was to investigate the antibiofilm and antimicrobial properties of metal and non-metal (Ag, Cu, Se) nanoparticles, obtained in deep eutectic solvent (DES), against resistant bacterial strains. The study investigated the determination of the minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of selected Me-DES systems. The results showed that the MBCs of <em>S. aureus</em> ATCC 700699 and <em>E. coli</em> ATCC BAA -2523 for Ag-DES were 18.2 and 15.6 μg/mL, respectively, while those for Cu-DES and Se-DES were 125 μg/mL. Serial passages of bacterial cells were performed to assess the ability of microorganisms to develop resistance to the test systems, indicating their limited adaptability against Ag-DES and Cu-DES (MIC maintenance for 24 passages). The antibiofilm activity of the nanoparticles was confirmed by their ability to remove 12–37 % of <em>S.Aureus</em> bacterial biofilm and 23–51 % of <em>E.coli</em> biofilm after 24 h of exposure. In addition, the MTT cytotoxicity test showed that Me-DES systems exhibited low cytotoxicity against cell lines (A549). Analysis of the mobility of microorganisms after exposure to nanoparticles indicated a reduction in mobility capacity in the range of 14–68 % already at a concentration of 31.3 μg/mL, depending on the nanoparticles used. The study demonstrates that metal and non-metal nanoparticles in DES represent a promising system to combat resistant microorganisms, while reducing their ability to develop resistance and destroying the bacterial biofilm.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106121"},"PeriodicalIF":4.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Roberta Ranaldi , Francesco Gabriele , Lorenza Rugnini , Patrick Di Martino , Rémy Agniel , Francesco Scuderi , Roberto Braglia , Antonella Canini , Nicoletta Spreti
{"title":"Essential oils in hydrogel for microalgal biofilm removal: Application strategies for stone heritage preservation","authors":"Roberta Ranaldi , Francesco Gabriele , Lorenza Rugnini , Patrick Di Martino , Rémy Agniel , Francesco Scuderi , Roberto Braglia , Antonella Canini , Nicoletta Spreti","doi":"10.1016/j.ibiod.2025.106128","DOIUrl":"10.1016/j.ibiod.2025.106128","url":null,"abstract":"<div><div>This study faces the critical need to develop new eco-friendly biocides and effective application strategies to mitigate phototrophic biodeteriogens in cultural heritage sites. To address this challenge, a microalgal strain previously collected from the hypogeum of the Colosseum (Rome, Italy) was used to induce biofilm formation on Lecce stone specimens. The samples were treated with 0.5% and 1% of essential oils (EOs) from <em>Thymus vulgaris</em> L., <em>Origanum vulgare</em> L. and <em>Cinnamomum verum</em> Presl. The EOs were encapsulated in an alginate hydrogel support matrix (HG) and applied to the biofilms with different application times (24 h and 48 h). A mini-PAM portable fluorometer was used to determine the phototrophic activity up to 2 months after treatment. Scanning electron microscopy was used to investigate the effects of EOs on microalgal cell morphology within biofilms, while Fourier transform infrared spectroscopy analyzed changes in biomolecular distribution after treatment. The findings revealed that all EOs were effective when encapsulated in HG. However, by the end of the monitoring period, only cinnamon EO maintained photosynthetic inhibition, especially when applied at 0.5% for 48 h. Moreover, biofilm treated with cinnamon EO showed the most significant effects, particularly in disrupting cell membranes and reducing lipids signals, ultimately leading to cell lysis. This approach effectively inhibited the vitality of biofilm-forming phototrophs on stone surfaces, using low concentrations of EO for defined periods of time.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106128"},"PeriodicalIF":4.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang-Yang Fan, Wen-Wen Liu, Yu-Han Chai, Jing Huang, Min Wang, Xin Wang, Xin-Lu Cai, Li Zhang, Xiang Xiao
{"title":"Innovative strategy for dechlorination of halogenated organic Pollutants: Microbial-Derived formate production and Immobilized-PdNP biosynthesis","authors":"Yang-Yang Fan, Wen-Wen Liu, Yu-Han Chai, Jing Huang, Min Wang, Xin Wang, Xin-Lu Cai, Li Zhang, Xiang Xiao","doi":"10.1016/j.ibiod.2025.106129","DOIUrl":"10.1016/j.ibiod.2025.106129","url":null,"abstract":"<div><div>Halogenated organic pollutants (HOPs) are accumulating in the environment, posing significant ecological risks. Microbial-driven palladium nanoparticles (PdNPs) facilitate efficient reductive hydrodehalogenation, offering a promising approach for HOPs remediation. To address the limitations associated with hydrogen donor addition and catalyst detachment, this study proposed a proof-of-concept strategy for developing a novel two-stage dehalogenation reactor. We initially constructed genetically engineered <em>Shewanella oneidensis</em> MR-1 to enhance the production and accumulation of formate as the hydrogen donor. Subsequently, encapsulated PdNPs was biosynthesized within alginate beads for efficient immobilization. Finally, a two-stage reactor was constructed for dechlorination of 2,4,6-trichlorophenol (2,4,6-TCP). In the first-stage reactor, the immobilized engineered strain accumulated 2.04 mM formate, providing sufficient hydrogen donors. In the second-stage reactor, 40 μM 2,4,6-TCP was completely dechlorinated within 10 h. Reactor performance was optimized through pH, lactate concentration, and initial pollutant load. Degradation product analysis revealed that dechlorination of 2,4,6-TCP was occurred via a one-electron transfer reaction, with the chlorinated intermediates being progressively hydrodechlorinated until the final product phenol. Furthermore, this constructed reactor exhibited high dechlorination efficiency for 2,4,6-TCP in real water samples and showed remediation potential for other HOPs. This study provides a promising strategy for the effective management of halogenated wastewater pollution.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106129"},"PeriodicalIF":4.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Melissa Christ , Nicole Flaig , Christian Brischke , Holger Militz , Marcus Müller
{"title":"Influence of polyethylene glycol and various carboxylic acids on the biological durability of beech wood (Fagus sylvatica) and Scots pine sapwood (Pinus sylvestris)","authors":"Melissa Christ , Nicole Flaig , Christian Brischke , Holger Militz , Marcus Müller","doi":"10.1016/j.ibiod.2025.106127","DOIUrl":"10.1016/j.ibiod.2025.106127","url":null,"abstract":"<div><div>The goal of this study was to investigate the impact of wood modification with polyethylene glycol (PEG) 400 and various carboxylic acids (citric acid (CA), malic acid (MA) and 1,2,3,4-butanetetracarboxylic acid (BTCA)) on the biological durability of beech wood (<em>Fagus sylvatica</em>) and Scots pine sapwood (<em>Pinus sylvestris</em>). For the brown, white and soft rot experiments, mass losses (ML) were calculated according to the respective standards. Weight percent gain, bulking and anti-swelling efficiency were determined to determine the quality of the modifications. Modifying agents, fixed inside the wood, demonstrated anti-swelling efficiency after leaching cycles (beech wood: 9–46 %). The decay resistance against brown and white rot fungi was considerably improved, with PEG/CA and PEG/BTCA being more effective than PEG/MA. Beech wood modified with PEG/CA achieved durability class (DC) 1–2, PEG/BTCA reached DC 1 and PEG/MA fell into DC 2–4. For Scots pine sapwood, PEG/CA and PEG/BTCA reached DC 1, while PEG/MA only achieved DC 4. The biological durability against soft rot fungi was less affected, and curing alone did not enhance the resistance to soft rot fungi. PEG/BTCA achieved DC 1, while PEG/MA and PEG/CA ranged between DC 1 and DC 3. The variants with different CA to PEG ratios as well as CA without PEG showed no major differences in terms of ML due to soft rot fungi. In conclusion, the modifications resulted in significantly lower mass losses.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106127"},"PeriodicalIF":4.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanglin Hu , Benxiang Guo , Yifei Wang , Fangwei Li , Xudong He , Qingyun Niu , Xiangyu Cui , Qingquan Wei , Xiaobang Hou , Lefeng Li
{"title":"Carbon steel Q235 corrosion protection by metabolism of Bacillus cereus biofilms in cooling water environment","authors":"Yanglin Hu , Benxiang Guo , Yifei Wang , Fangwei Li , Xudong He , Qingyun Niu , Xiangyu Cui , Qingquan Wei , Xiaobang Hou , Lefeng Li","doi":"10.1016/j.ibiod.2025.106124","DOIUrl":"10.1016/j.ibiod.2025.106124","url":null,"abstract":"<div><div>Multiple types of microorganisms have been reported to provide a corrosion protective effect on metals, but few cases under cooling water environments. This study addresses this research gap by employing <em>Bacillus cereus</em> (<em>B. cereus</em>) biofilms for carbon steel (CS) Q235 corrosion protection. The results demonstrated that the addition of <em>B. cereus</em> agent reduced the weight loss of CS Q235 by 92.38 %, increased the electrochemical impedance (<em>R</em><sub><em>ct</em></sub> 21616.77 Ω/cm<sup>2</sup>) and lowered the corrosion current density (<em>I</em><sub><em>corr</em></sub> 16.8 μA cm<sup>−2</sup>). Surface analysis indicated that <em>B. cereus</em> cells secreted polysaccharides and proteins to form a biofilm. Such biofilm, combined with biomineralized corrosion products, formed a composite layer that prevented direct contact between CS Q235 and corrosive media. Moreover, metabolites of <em>B. cereus</em> biofilm associated with corrosion protective capacity were profiled and screen by untargeted metabolism analysis. A total of 376 metabolites were detected and genetic materials including uridine monophosphate (UMP) and guanosine monophosphate (GMP) were identified as the most critical metabolic principle for corrosion protection. These metabolites, which enriched in nucleotide metabolic pathways, was considered as metabolic mechanisms of corrosion protection. The <em>B. cereus</em> cells forming extracellular polymeric substance (EPS)-biofilm to alter the physicochemical properties of CS Q235 surface and repelled the attachment of corrosive microorganisms, thus hindered their multiplication and protected CS Q235 from corrosion in cooling water environment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"203 ","pages":"Article 106124"},"PeriodicalIF":4.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}