Inhalation Toxicology最新文献_第7页

Analysis of abnormal expression of signaling pathways in PQ-induced acute lung injury in SD rats based on RNA-seq technology. 基于 RNA-seq 技术分析 PQ 诱导 SD 大鼠急性肺损伤信号通路的异常表达。

IF 2 4区医学

Inhalation Toxicology Pub Date : 2024-01-01 Epub Date: 2024-01-04 DOI: 10.1080/08958378.2023.2300373

Nan Li, Yue Huang, Yang Yi, Jin Qian, Qi Li, Shuang-Qin Xu, Hang-Fei Wang, Xin-Xin Wu, Ji-Chao Peng, Li-Hua Li, Jin-Jian Yao, Xiao-Ran Liu

{"title":"Analysis of abnormal expression of signaling pathways in PQ-induced acute lung injury in SD rats based on RNA-seq technology.","authors":"Nan Li, Yue Huang, Yang Yi, Jin Qian, Qi Li, Shuang-Qin Xu, Hang-Fei Wang, Xin-Xin Wu, Ji-Chao Peng, Li-Hua Li, Jin-Jian Yao, Xiao-Ran Liu","doi":"10.1080/08958378.2023.2300373","DOIUrl":"10.1080/08958378.2023.2300373","url":null,"abstract":"Background: Paraquat (PQ) plays an important role in agricultural production due to its highly effective herbicidal effect. However, it has led to multiple organ failure in those who have been poisoned, with damage most notable in the lungs and ultimately leading to death. Because of little research has been performed at the genetic level, and therefore, the specific genetic changes caused by PQ exposure are unclear.Methods: Paraquat poisoning model was constructed in Sprague Dawley (SD) rats, and SD rats were randomly divided into Control group, paraquat (PQ) poisoning group and Anthrahydroquinone-2,6-disulfonate (AH2QDS) treatment group. Then, the data was screened and quality controlled, compared with reference genes, optimized gene structure, enriched at the gene expression level, and finally, signal pathways with significantly different gene enrichment were screened.Results: This review reports on lung tissues from paraquat-intoxicated Sprague Dawley (SD) rats that were subjected to RNA-seq, the differentially expressed genes were mainly enriched in PI3K-AKT, cGMP-PKG, MAPK, Focal adhesion and other signaling pathways.Conclusion: The signaling pathways enriched with these differentially expressed genes are summarized, and the important mechanisms mediated through these pathways in acute lung injury during paraquat poisoning are outlined to identify important targets for AH2QDS treatment of acute lung injury due to paraquat exposure, information that will be used to support a subsequent in-depth study on the mechanism of PQ action.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139086714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjuvant effect of inhaled particulate matter containing free radicals following house-dust mite induction of asthma in mice 屋尘螨诱发小鼠哮喘后，吸入含有自由基的微粒物质的辅助作用

IF 2.1 4区医学

Inhalation Toxicology Pub Date : 2023-12-07 DOI: 10.1080/08958378.2023.2289024

Alexandra Noël, Ashlyn C. Harmon, Balamurugan Subramanian, Zakia Perveen, Ankit Aryal, Kelsey Legendre, Hasan Zaman, Daniel B. Paulsen, Kurt J. Varner, Tammy R. Dugas, Arthur L. Penn

引用次数: 0

Toxic chemical exposures among civilians in armed conflicts: the need for research equity, justice, and accountability 武装冲突中平民的有毒化学品接触：研究公平、正义和问责的必要性

IF 2.1 4区医学

Inhalation Toxicology Pub Date : 2023-12-07 DOI: 10.1080/08958378.2023.2286325

Sammy Almashat, Melissa McDiarmid

引用次数: 0

Vaping product exposure system (VaPES): a novel in vitro aerosol deposition system. Vaping 产品暴露系统 (VaPES)：一种新型体外气溶胶沉积系统。

IF 2 4区医学

Inhalation Toxicology Pub Date : 2023-12-01 Epub Date: 2023-12-13 DOI: 10.1080/08958378.2023.2289021

Kevin D Schichlein, Charlotte A Love, Maxwell P Conolly, John L Kurz, Elise D Hickman, Phillip W Clapp, Ilona Jaspers

{"title":"Vaping product exposure system (VaPES): a novel in vitro aerosol deposition system.","authors":"Kevin D Schichlein, Charlotte A Love, Maxwell P Conolly, John L Kurz, Elise D Hickman, Phillip W Clapp, Ilona Jaspers","doi":"10.1080/08958378.2023.2289021","DOIUrl":"10.1080/08958378.2023.2289021","url":null,"abstract":"Objective: Due to recent increases in the use of vaping devices, there is a high demand for research addressing the respiratory health effects of vaping products. Given the constantly changing nature of the vaping market with new devices, flavors, metals, and other chemicals rapidly emerging, there is a need for inexpensive and highly adaptable vaping device exposure systems. Here, we describe the design and validation of a novel in vitro aerosol exposure system for toxicity testing of vaping devices.Materials and methods: We developed an inexpensive, open-source in vitro vaping device exposure system that produces even deposition, can be adapted for different vaping devices, and allows for experiments to be performed under physiological conditions. The system was then validated with deposition testing and a representative exposure with human bronchial epithelial cells (hBECs).Results: The Vaping Product Exposure System (VaPES) produced sufficient and uniform deposition for dose-response studies and was precise enough to observe biological responses to vaping exposures. VaPES was adapted to work with both pod and cartridge-based vaping devices.Conclusion: We have designed and validated a novel vaping device exposure system that will eliminate the need to use high-cost commercial exposure systems, lowering the barrier to entry of physiologically relevant vaping studies.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":" ","pages":"324-332"},"PeriodicalIF":2.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10788097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138487390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Airway contraction and cytokine release in isolated rat lungs induced by wear particles from the road and tire interface and road vehicle brakes. 路面和轮胎界面以及公路车辆制动器磨损颗粒诱导离体大鼠肺部气道收缩和细胞因子释放。

IF 2 4区医学

Inhalation Toxicology Pub Date : 2023-12-01 Epub Date: 2023-12-13 DOI: 10.1080/08958378.2023.2289018

Ali Reza Nosratabadi, Mats Gustafsson, Karin Lovén, Stefan A Ljunggren, Ulf Olofsson, Saeed Abbasi, Göran Blomqvist, Helen Karlsson, Anders G Ljungman, Flemming R Cassee, Miriam E Gerlofs-Nijland, Anders Gudmundsson

{"title":"Airway contraction and cytokine release in isolated rat lungs induced by wear particles from the road and tire interface and road vehicle brakes.","authors":"Ali Reza Nosratabadi, Mats Gustafsson, Karin Lovén, Stefan A Ljunggren, Ulf Olofsson, Saeed Abbasi, Göran Blomqvist, Helen Karlsson, Anders G Ljungman, Flemming R Cassee, Miriam E Gerlofs-Nijland, Anders Gudmundsson","doi":"10.1080/08958378.2023.2289018","DOIUrl":"10.1080/08958378.2023.2289018","url":null,"abstract":"The dominant road traffic particle sources are wear particles from the road and tire interface, and from vehicle brake pads. The aim of this work was to investigate the effect of road and brake wear particles on pulmonary function and biomarkers in isolated perfused rat lungs. Particles were sampled from the studded tire wear of three road pavements containing different rock materials in a road simulator; and from the wear of two brake pad materials using a pin-on-disk machine. Isolated rat lungs inhaled the coarse and fine fractions of the sampled particles resulting in an estimated total particle lung dose of 50 μg. The tidal volume (TV) was measured during the particle exposure and the following 50 min. Perfusate and BALF were analyzed for the cytokines TNF, CXCL1 and CCL3. The TV of lungs exposed to rock materials was significantly reduced after 25 min of exposure compared to the controls, for quartzite already after 4 min. The particles of the heavy-duty brake pads had no effect on the TV. Brake particles resulted in a significant elevation of CXCL1 in the perfusate. Brake particles showed significant elevations of all three measured cytokines, and quartzite showed a significant elevation of TNF in BALF. The study shows that the toxic effect on lungs exposed to airborne particles can be investigated using measurements of tidal volume. Furthermore, the study shows that the choice of rock material in road pavements has the potential to affect the toxicity of road wear PM10.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":" ","pages":"309-323"},"PeriodicalIF":2.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138487389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time to treat the climate and nature crisis as one indivisible global health emergency. 是时候将气候和自然危机视为一个不可分割的全球卫生紧急事件了。

IF 2.1 4区医学

Inhalation Toxicology Pub Date : 2023-10-01 Epub Date: 2023-12-07 DOI: 10.1080/08958378.2023.2276512

Kamran Abbasi, Parveen Ali, Virginia Barbour, Thomas Benfield, Kirsten Bibbins-Domingo, Gregory E Erhabor, Stephen Hancocks, Richard Horton, Laurie Laybourn-Langton, Robert Mash, Peush Sahni, Wadeia Mohammad Sharief, Paul Yonga, Chris Zielinski

引用次数: 0

Development of a non-target strategy for evaluation of potential biological effects of inhalable aerosols generated during purposeful room conditioning using an in vitro inhalation model. 开发一种非目标策略，使用体外吸入模型评估有目的的房间调节过程中产生的可吸入气溶胶的潜在生物效应。

IF 2 4区医学

Inhalation Toxicology Pub Date : 2023-10-01 Epub Date: 2023-12-07 DOI: 10.1080/08958378.2023.2267618

Detlef Ritter, Jan Knebel, Tanja Hansen, Anne Zifle, Anne Fuchs, Rolf Fautz, Katharina Schwarz

{"title":"Development of a non-target strategy for evaluation of potential biological effects of inhalable aerosols generated during purposeful room conditioning using an in vitro inhalation model.","authors":"Detlef Ritter, Jan Knebel, Tanja Hansen, Anne Zifle, Anne Fuchs, Rolf Fautz, Katharina Schwarz","doi":"10.1080/08958378.2023.2267618","DOIUrl":"10.1080/08958378.2023.2267618","url":null,"abstract":"Objectives: An integrated in vitro inhalation approach was outlined to estimate potential adverse acute inhalation effects of aerosols from commercial nebulizer applications used for purposeful room conditioning such as disinfection, scenting or others. Aerosol characterization, exposure estimation and evaluation of acute biological effects by in vitro inhalation were included to generate dose-response data, allowing for determination of in vitro lowest observable adverse effect levels (LOAELs). Correlation of these to estimates of human lung deposition was included for quantitative in vitro to in vivo extrapolation approach (QIVIVE) for acute effects during human exposure.Methods: To test the proposed approach, a case study was undertaken using two realistic test materials. An acute in vitro inhalation setup with air-liquid interface A549-cells in an optimized exposure situation (P.R.I.T.® ExpoCube®) was used to expose cells and analysis of relevant biological effects (viability, mitochondrial membrane potential, stress, IL-8 release) was carried out.Results: The observed dose-responsive effects in a sub-toxic dose-range could be attributed to the main component of one test material and its presence in the aerosol phase of the nebulized material. QIVIVE resulted in a factor of at least 256 between the in vitro LOAEL and the estimated acute human lung exposure for this test material.Conclusions: The case-study shows the value of the non-target in vitro inhalation testing approach especially in case of a lack of knowledge on complex product composition. It is expected that approaches like this will be of high value for product safety and environmental health in the future.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":" ","pages":"271-284"},"PeriodicalIF":2.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49677125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of exposure to methylene diphenyl-4,4'-diisocyanate (MDI) aerosol using computational fluid dynamics, physiologically based toxicokinetics and statistical modeling. 利用计算流体动力学、基于生理的毒物动力学和统计模型重建暴露于亚甲基二苯基-4,4'-二异氰酸酯(MDI)气溶胶的过程。

IF 2 4区医学

Inhalation Toxicology Pub Date : 2023-10-01 Epub Date: 2023-12-07 DOI: 10.1080/08958378.2023.2285772

Sajjad Mozaffari, Majid Bayatian, Nan-Hung Hsieh, Monireh Khadem, Amir Abbasi Garmaroudi, Khosro Ashrafi, Seyed Jamaleddin Shahtaheri

{"title":"Reconstruction of exposure to methylene diphenyl-4,4'-diisocyanate (MDI) aerosol using computational fluid dynamics, physiologically based toxicokinetics and statistical modeling.","authors":"Sajjad Mozaffari, Majid Bayatian, Nan-Hung Hsieh, Monireh Khadem, Amir Abbasi Garmaroudi, Khosro Ashrafi, Seyed Jamaleddin Shahtaheri","doi":"10.1080/08958378.2023.2285772","DOIUrl":"10.1080/08958378.2023.2285772","url":null,"abstract":"Objectives: This study employed computational fluid dynamics (CFD), physiologically based toxicokinetics (PBTK), and statistical modeling to reconstruct exposure to methylene diphenyl-4,4'-diisocyanate (MDI) aerosol. By utilizing a validated CFD model, human respiratory deposition of MDI aerosol in different workload conditions was investigated, while a PBTK model was calibrated using experimental rat data. Biomonitoring data and Markov Chain Monte Carlo (MCMC) simulation were utilized for exposure assessment.Results: Deposition fraction of MDI in the respiratory tract at the light, moderate, and heavy activity were 0.038, 0.079, and 0.153, respectively. Converged MCMC results as the posterior means and prior values were obtained for several PBTK model parameters. In our study, we calibrated a rat model to investigate the transport, absorption, and elimination of 4,4'-MDI via inhalation exposure. The calibration process successfully captured experimental data in the lungs, liver, blood, and kidneys, allowing for a reasonable representation of MDI distribution within the rat model. Our calibrated model also represents MDI dynamics in the bloodstream, facilitating the assessment of bioavailability. For human exposure, we validated the model for recent and long-term MDI exposure using data from relevant studies.Conclusion: Our computational models provide reasonable insights into MDI exposure, contributing to informed risk assessment and the development of effective exposure reduction strategies.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":" ","pages":"285-299"},"PeriodicalIF":2.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138459747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung asbestos fiber burden analysis: effects of the counting rules for legal medicine evaluations. 肺石棉纤维负荷分析:法医学评价计数规则的效果。

IF 2 4区医学

Inhalation Toxicology Pub Date : 2023-10-01 Epub Date: 2023-12-07 DOI: 10.1080/08958378.2023.2285789

Anna Benedetta Somigliana, Pietro Gino Barbieri, Alessandro Cavallo, Roberto Colombo, Dario Consonni, Dario Mirabelli

{"title":"Lung asbestos fiber burden analysis: effects of the counting rules for legal medicine evaluations.","authors":"Anna Benedetta Somigliana, Pietro Gino Barbieri, Alessandro Cavallo, Roberto Colombo, Dario Consonni, Dario Mirabelli","doi":"10.1080/08958378.2023.2285789","DOIUrl":"10.1080/08958378.2023.2285789","url":null,"abstract":"Objectives: The work shows the effect of counting rules, such as analysis magnification and asbestos fiber dimension to be count (with length ≥5 µm or also asbestos fibers with length <5 µm) in the lung asbestos fiber burden analysis for legal medicine evaluations.Methods: On the same lung tissue samples, two different analyses were carried out to count any asbestos fibers with length ≥1 µm and with length ≥5 µm. Results of the amphibole burden of the two analyses were compared by linear regression analysis on log10-transformed values.Results: The analysis should be carried out at an appropriate magnification and on samples prepared in such a way as they allow the counting of very fine fibers. If the analysis is limited to the asbestos fibers with length ≥5 µm, there is a high risk of not detecting possible residual chrysotile fiber burden and thinner crocidolite asbestos fibers.Conclusions: On average we estimated that 1 amphibole fiber with length ≥5 µm corresponds to ∼8 amphibole fibers with length ≥1 µm in the lung. The values of the Helsinki criteria should be updated taking this into account.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":" ","pages":"300-307"},"PeriodicalIF":2.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138295151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of aerosols from hemp-derived pre-roll joints. 来自大麻衍生的预滚接头的气溶胶特性。

IF 2.1 4区医学

Inhalation Toxicology Pub Date : 2023-05-01 DOI: 10.1080/08958378.2023.2206448

Hammad Irshad, Justyna Kulpa, Philip J Kuehl, Tim Lefever, Jacob D McDonald

{"title":"Characterization of aerosols from hemp-derived pre-roll joints.","authors":"Hammad Irshad, Justyna Kulpa, Philip J Kuehl, Tim Lefever, Jacob D McDonald","doi":"10.1080/08958378.2023.2206448","DOIUrl":"https://doi.org/10.1080/08958378.2023.2206448","url":null,"abstract":"Objective: Availability and consumer use of hemp products is rapidly increasing, but little work has been done to assess aerosol emissions of hemp pre-rolls. The objective of this research was to characterize the aerosol of pre-rolled joints from hemp material enriched for production of cannabigerol (CBG) that were smoked on a test system mimicking human use patterns.Materials and methods: Aerosol emissions were collected and analyzed using glass microfiber filters and charcoal cartridges. The aerosol was screened for nine phytocannabinoids and 19 terpenes.Results: Three phytocannabinoids (CBG, cannabichromene (CBC), and delta-9-tetrahydrocannabinol (THC)) were detected and quantified at a mean (SD) concentration of 19.4 (4.7), 0.48 (0.01), and 0.40 (0.04) mg per pre-roll, respectively. Five terpenes ((-)-α-bisabolol, (-)-guaiol, β-caryophyllene, nerolidol, and α-humulene) were detected and quantified at an average concentration of 352.7 (112.0), 194.3 (66.4), 106.0 (50.4), 28.3 (9.3), and 27.7 (11.2) µg per pre-roll, respectively. Particle size distribution testing via aerodynamic particle sizer and inertial impactor showed that average size of emitted aerosols was 0.77 (0.0) and 0.54 (0.1) µm, respectively.Conclusions: This study describes methodology for characterization of cannabinoid and terpene dose in emitted aerosols and aerosolization efficiency from hemp pre-rolls. It also presents these data for one of the marketed products.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":"35 5-6","pages":"169-174"},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9506967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0