Lung asbestos fiber burden analysis: effects of the counting rules for legal medicine evaluations.

IF 2 4区医学 Q4 TOXICOLOGY

Inhalation Toxicology Pub Date : 2023-10-01 Epub Date: 2023-12-07 DOI:10.1080/08958378.2023.2285789

Anna Benedetta Somigliana, Pietro Gino Barbieri, Alessandro Cavallo, Roberto Colombo, Dario Consonni, Dario Mirabelli

{"title":"Lung asbestos fiber burden analysis: effects of the counting rules for legal medicine evaluations.","authors":"Anna Benedetta Somigliana, Pietro Gino Barbieri, Alessandro Cavallo, Roberto Colombo, Dario Consonni, Dario Mirabelli","doi":"10.1080/08958378.2023.2285789","DOIUrl":null,"url":null,"abstract":"Objectives: The work shows the effect of counting rules, such as analysis magnification and asbestos fiber dimension to be count (with length ≥5 µm or also asbestos fibers with length <5 µm) in the lung asbestos fiber burden analysis for legal medicine evaluations.Methods: On the same lung tissue samples, two different analyses were carried out to count any asbestos fibers with length ≥1 µm and with length ≥5 µm. Results of the amphibole burden of the two analyses were compared by linear regression analysis on log10-transformed values.Results: The analysis should be carried out at an appropriate magnification and on samples prepared in such a way as they allow the counting of very fine fibers. If the analysis is limited to the asbestos fibers with length ≥5 µm, there is a high risk of not detecting possible residual chrysotile fiber burden and thinner crocidolite asbestos fibers.Conclusions: On average we estimated that 1 amphibole fiber with length ≥5 µm corresponds to ∼8 amphibole fibers with length ≥1 µm in the lung. The values of the Helsinki criteria should be updated taking this into account.","PeriodicalId":13561,"journal":{"name":"Inhalation Toxicology","volume":" ","pages":"300-307"},"PeriodicalIF":2.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Inhalation Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/08958378.2023.2285789","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/7 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: The work shows the effect of counting rules, such as analysis magnification and asbestos fiber dimension to be count (with length ≥5 µm or also asbestos fibers with length <5 µm) in the lung asbestos fiber burden analysis for legal medicine evaluations.

Methods: On the same lung tissue samples, two different analyses were carried out to count any asbestos fibers with length ≥1 µm and with length ≥5 µm. Results of the amphibole burden of the two analyses were compared by linear regression analysis on log10-transformed values.

Results: The analysis should be carried out at an appropriate magnification and on samples prepared in such a way as they allow the counting of very fine fibers. If the analysis is limited to the asbestos fibers with length ≥5 µm, there is a high risk of not detecting possible residual chrysotile fiber burden and thinner crocidolite asbestos fibers.

Conclusions: On average we estimated that 1 amphibole fiber with length ≥5 µm corresponds to ∼8 amphibole fibers with length ≥1 µm in the lung. The values of the Helsinki criteria should be updated taking this into account.

查看原文本刊更多论文

肺石棉纤维负荷分析:法医学评价计数规则的效果。

目的:本工作显示了计数规则的影响，如分析放大倍数和要计数的石棉纤维尺寸(长度≥5µm或石棉纤维长度)。方法:对同一肺组织样本，进行两种不同的分析，以计数长度≥1µm和长度≥5µm的石棉纤维。对两种分析的角闪孔负荷进行log10变换值的线性回归分析比较。结果:分析应在适当的放大倍率下进行，并对样品进行制备，使其能够计数非常细的纤维。如果分析仅限于长度≥5µm的石棉纤维，则有很高的风险无法检测到可能残留的温石棉纤维负荷和较薄的青石棉纤维。结论:我们估计平均1个长度≥5µm的角闪孔纤维对应于肺中长度≥1µm的~ 8个角闪孔纤维。应考虑到这一点，更新赫尔辛基标准的值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Inhalation Toxicology 医学-毒理学

CiteScore

4.10

自引率

4.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Inhalation Toxicology is a peer-reviewed publication providing a key forum for the latest accomplishments and advancements in concepts, approaches, and procedures presently being used to evaluate the health risk associated with airborne chemicals. The journal publishes original research, reviews, symposia, and workshop topics involving the respiratory system’s functions in health and disease, the pathogenesis and mechanism of injury, the extrapolation of animal data to humans, the effects of inhaled substances on extra-pulmonary systems, as well as reliable and innovative models for predicting human disease.