Immunopathologia Persa最新文献

{"title":"IL-17A rs2275913 gene polymorphism in patients with diabetic nephropathy","authors":"Akbar Nazarian, S. M. Hejazian, E. Ahmadian, S. Zununi Vahed, M. Haghi, M. Mobasseri, M. Ardalan","doi":"10.34172/ipp.2022.29320","DOIUrl":"https://doi.org/10.34172/ipp.2022.29320","url":null,"abstract":"Introduction: Diabetic nephropathy (DN) is a major microvascular complication of diabetes, leading to end-stage renal disease (ESRD). Objectives: The aim of this study was to investigate the association between the rs2275913 polymorphism of interleukin-17 (IL-17) gene and the incidence of kidney disease in patients with type 2 diabetes (T2D). Patients and Methods: Blood samples were collected from 113 T2D patients including 56 patients with nephropathy and 57 patients without nephropathy. In addition, 150 healthy individuals were included in this study to compare the results. Gene study was conducted by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) method. Results: The frequency of A allele of IL-17A (rs2275913) gene polymorphism was significantly higher in patients with DN compared to healthy controls (P=0.043). In addition, serum creatinine levels were significantly higher in DN patients regardless of their genotypes (P<0.001). Conclusion: Our results showed that diabetic patients who carry at least one A allele of IL-17A (rs2275913) gene polymorphism may develop DN.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46357629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the analgesic and sedative effects of intravenous ketamine versus morphine administration on relieving long/short bone-fracture pain in the upper/ lower limbs; a phase II clinical trial","authors":"H. Motamed, Meisam Moezi, Azam Khilghi, M. Fahimi","doi":"10.34172/ipp.2022.30334","DOIUrl":"https://doi.org/10.34172/ipp.2022.30334","url":null,"abstract":"Introduction: The importance of pain control in patients with limb trauma admitted to emergency departments as well as its complications is among the main concerns in post-emergency care, which contributes to the accelerated improvement of patients’ conditions in a significant manner. Objectives: The present study was to evaluate the analgesic and sedative effects of intravenous (IV) ketamine versus morphine administration on relieving long/short bone-fracture pain in the upper/lower limbs. Patients and Methods: The present study is as a double-blind randomized clinical trial. For this purpose, the effect of ketamine and morphine were initially examined using IV ketamine and morphine administration, respectively, at the doses of 0.4 and 0.1 mg/kg/IV/10 min in patients, aged 18-65 years with limb trauma, and admitted to hospital emergency departments. Afterward, the duration of the analgesic effect, the amount of pain relief, according to the visual analog scale (VAS) outcomes, and the complications for each drug, including apnea, bradycardia, tachycardia, altered level of consciousness, nausea, vomiting, hypertension/hypotension, seizures and disturbed sleep were compared, and then the preferred method was introduced. Results: In this study, 120 patients in total, including 60 cases receiving ketamine and 60 individuals receiving morphine, were recruited. The participants’ age range was between 19 -70 years. The patients’ mean age was 47.04±12.57 years of whom 89 patients (74.2%) were male. The study results indicated that the potency of the low-dose ketamine infusion in relieving pain in patients was comparable to that of morphine. Conclusion: It was concluded that ketamine could be administered as an alternative to IV morphine to reduce long/short bone-fracture pain in the upper/lower limbs. Trial Registration: The trial protocol was authorized by the Iranian Registry of Clinical Trials, (identifier: IRCT20170716035105N3; https://en.irct.ir/trial/26628, ethical code: IR.AJUMS.REC.1396.248).","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42968778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The STAT4 SNP (rs7574865) and systemic lupus erythematosus","authors":"G. Bayat, S. M. Hejazian, E. Ahmadian, Seyed Sina Hejazian, A. Khabbazi, S. Zununi Vahed, M. Ardalan","doi":"10.34172/ipp.2022.29321","DOIUrl":"https://doi.org/10.34172/ipp.2022.29321","url":null,"abstract":"Introduction: Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease affecting several systems and organs in the body. The association of STAT4 transcription factor with SLE risk remains unclear. Objectives: The aim of this study was to investigate the association of STAT4 gene polymorphism (rs7574865) with the incidence of SLE. Patients and Methods: One hundred and sixty participants (80 patients with SLE and 80 healthy individuals) were included in this study. Gene analysis was conducted by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) in peripheral blood samples. Results: Fifty-seven percent (n=45) of patients with SLE had SLE disease activity index (SLEDAI) above six and had active disease. In the SLE group, the frequency of G and T alleles were 81% and 19%, respectively. Moreover, 72.50% (n=58) of patients carried the GG genotype, 17.5% (n=14) had the GT genotype and 10.1% (n=8) carried the TT genotype. There was no significant difference between allele frequency and genotypic distribution for rs7574865 polymorphism (P>0.05) between SLE and control groups. Significant differences were observed between the distribution of genotypes and clinical manifestations including leukopenia (P=0.04), pulmonary (P=0.01) and ophthalmic (P=0.049) problems. The T allele with an odd ratio of 1.47 and confidence interval of 0.80 to 2.6 could increase the risk of SLE; however, it was not statistically significant (P=0.20). Conclusion: The T allele and TT genotype of the STAT4 rs7574865 polymorphism could increase the risk of lupus; however, these observations were not statistically significant.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48058055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation the expression of insulin and insulin receptor-beta (IR-β) in sperm of infertile male with failed intracytoplasmic sperm injection (ICSI)","authors":"Fateme Alizadeh, M. Hemadi, G. Saki, Akram Gavahi, F. Moramezi, A. Valizadeh","doi":"10.34172/ipp.2022.7162","DOIUrl":"https://doi.org/10.34172/ipp.2022.7162","url":null,"abstract":"Introduction: Most infertility treatment centers are currently using semen analysis to differentiate between fertile and infertile individuals. Recent studies have evaluated the expression and secretion of independent insulin and related beta receptor (IR-β) in mammals’ ejaculated spermatozoa such as humans and pigs, and its effect on motility parameters capacitation and acrosomal reaction. Objectives: This study aimed to investigate the expression of insulin, IR-β and the parameters of sperm and DNA fragmentation index (DFI) in men with intracytoplasmic sperm injections (ICSI) failed. Patients and Methods: This study was conducted on 15 men with unknown infertility with a history of unsuccessful ICSI and 10 fertile men. After collecting samples, sperm parameters (concentration and motility), sperm DNA fragmentation and expression of insulin and IR-β genes were investigated. To assess DFI from SDFA kit and to review the expression, the genes were analyzed via RT-PCR method. Data were analyzed statistically by t-test and covariance tests. Results: Based on the findings of this research, the concentration and percentage of motility in infertile men with a history of unsuccessful ICSI was significantly lower than fertile individuals, moreover, the amount of DNA fragmentation significantly increased in infertile men with a history of unsuccessful ICSI compared to fertile men (P < 0.05). Insulin expression in the sperm of infertile men with a failed ICSI history was significantly lower than fertile men (P < 0.05); however, the IR-β expression rate was not significantly different between the two groups. Conclusion: Evaluation of insulin gene expression can be helpful as a good marker for identifying infertility with an unknown cause.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42230113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cost-effectiveness analysis of infliximab versus CinnoRA in the treatment of moderate to severe ulcerative colitis in Iranian patients","authors":"Meysam Olfatifar, H. Asadzadeh Aghdaei, Ayda Hasanpour Dehkordi, S. Shahrokh, M. Pourhoseingholi, H. Balaii, Mohsen Rajabnia, M. Ivanchuk, P. Ivanchuk, Saeed Hashemi Nazari, S. Sabour, P. Rohani, G. Mehralian, S. Khodakarim, B. Hatami, Habib Malekpour, Ghazal Sherkat, M. Zali, Sajjad Rahimi Pordanjani","doi":"10.34172/ipp.2022.29293","DOIUrl":"https://doi.org/10.34172/ipp.2022.29293","url":null,"abstract":"Introduction: As two biological agents, infliximab (IFX) and biosimilar adalimumab (CinnoRA®) are routinely used in the clinical management of ulcerative colitis (UC) in Iran. Objectives: This study was done to evaluate the cost-effectiveness of IFX versus CinnoRA for the treatment of moderate-to-severe UC patients. Patients and Methods: To accomplish this, we developed a hybrid decision-tree/microsimulation (MS) approach for modeling UC’s natural history. We populated our model with available data on probabilities, costs, utilities / disutilities, and emergent adverse effects. Costs were reported in Iranian Rial (IRR) and in April 2021 US dollars ($). One-way and multiple sensitivity analyses were used to determine the uncertainty of the model’s parameters. Results: For five, 10, and lifetime horizon times, patients on IFX received slightly more quality-adjusted life-year (QALY) per year in remission and experienced about 3 to 5 times less surgery than CinnoRA patients. With willingness-to-pay (WTP) thresholds of 1800 ($7826.08), 820($3565.21), and 520 ($2260.86) million IRR for these horizon times, IFX was cost-effective with 100% certainty. Our findings were highly sensitive to the number of adverse effects. Conclusion: Our results demonstrated that IFX is more effective and more costly than CinnoRA, and if we ignore the predicted surgeries, CinnoRA is nearly as effective as IFX. However, these findings should be cautiously interpreted without a robust clinical trial of CinnoRA in UC patients. Since the impact of CinnoRA may have been over/underestimated.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44528963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological diversity of the corneal small fibers in patients with type 1 diabetes mellitus","authors":"Anna Bregovskaya, M. Lukashenko, E. Kamaeva, N. Gavrilova, L. Soprun, N. Grigoryeva, I. Pchelin, L. Churilov","doi":"10.34172/ipp.2022.31336","DOIUrl":"https://doi.org/10.34172/ipp.2022.31336","url":null,"abstract":"Introduction: Diabetic neuropathy (DN) affects up to 50% of patients with type 1 diabetes mellitus (T1DM). Clinical manifestations include painful polyneuropathy with autonomic and orthostatic symptoms. Corneal confocal microscopy (CCM) is one of the promising non-invasive methods for DN evaluation and tracking the effectiveness of treatment. Objectives: To perform the early diagnosis of DN in T1DM patients and describe the morphological changes in nerve fibers during the treatment process. Patients and Methods: This prospective study included 77 T1DM patients and 42 healthy controls. All patients underwent CCM and electroneuromyography (ENMG). Twenty-five patients with T1DM and HbA1c 7-10% underwent strict glycemic control with the correction of insulin therapy and the subsequent CCM and ENMG investigations for the second time. Results: In T1DM patients a decrease in the main nerve trunks with age was revealed, as well as an increase in corneal nerve tortuosity (P<0.05). The number of branches of nerve fibers did not change with the increasing age (P=0.07). After reaching T1DM compensation, patients underwent a course of alpha-lipoic acid therapy. Confocal microscopy of the cornea parameters did not undergo significant changes during therapy since significant positive dynamics were noted only in the tortuosity of nerve fibers (P<0.05), which decreased by 11.8%. Conclusion: In patients with T1DM, strict glycemic control, correction of insulin therapy, and courses of alpha-lipoic acid prescription help to reduce the morphological changes of small fiber neuropathy, which can be evaluated by the confocal microscopy of the cornea.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45918481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoimmune manifestations of the post-COVID-19 condition","authors":"V. Ryabkova, Anna Bregovskaya, L. Soprun, N. Gavrilova, L. Churilov","doi":"10.34172/ipp.2022.31339","DOIUrl":"https://doi.org/10.34172/ipp.2022.31339","url":null,"abstract":"In 2020, the world suffered an epidemic of a new coronavirus infection, after which it became obvious that the consequences of this disease persist for a long time after the recovery, perhaps, for several months. This complication was called post-COVID-19 syndrome and was recognized by the World Health Organization, which was reflected in the Delphi Consensus on October 6, 2021. The clinical characteristics of this disease include a significant number of immunological manifestations, including fever, arthralgia, myalgia, flu-like symptoms, as well as autoimmune diseases manifestation. These symptoms may allude the autoimmune genesis of the COVID-19 complications. This mini-review summarizes the current understanding of the systemic manifestations of COVID-19 and the possible role of impaired immune function in the pathogenesis of post-COVID-19 syndrome.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45547969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of ultrasound findings and fine needle aspiration results between anti-thyroid peroxidase positive and negative patients with nodular goiters","authors":"M. Malekian, Nasser Aghamohammadzadeh, F. Najafipour, Reza Javad-Rashid, J. Jalili, M. Halimi, F. Bozorgi, Jalil Houshyar","doi":"10.34172/ipp.2022.24257","DOIUrl":"https://doi.org/10.34172/ipp.2022.24257","url":null,"abstract":"Introduction: Ultrasonography is an optimal approach for thyroid gland evaluation, nodule detection and cancer risk assessment in patients with thyroid nodules. Almost all patients with Hashimoto’s thyroiditis have high levels anti-thyroid peroxidase (anti-TPO) antibodies. Objectives: This study aimed to evaluate the relationship between ultrasonography and fine needle aspiration (FNA) results in anti-TPO antibodies positive and negative patients with nodular goiters. Patients and Methods: This cross-sectional study included 128 patients with nodular goiters, referred to endocrinology clinic of imam Reza hospital, Tabriz, Iran. Anti-TPO levels above and below 16 IU/mL were considered as positive and negative, respectively. All patients underwent thyroid ultrasonography, and eligible nodules were subjected to FNA. Results: Of 128 patients, 33.6% and 66.4% were anti-TPO positive and negative, respectively. FNA was conducted on 196 nodules. A significant relationship was observed between sonographic and FNA results in low and intermediate-suspicion nodules. Chronic lymphocytic thyroiditis (CLT) was more frequently reported in low-suspicion nodules of the anti-TPO positive group (P≤0.0001). In addition, in intermediate-suspicion nodules, CLT was reported in 33.3% of patients in the anti-TPO positive group (P=0.026). No significant difference was observed between other nodules. Conclusion: Based on our findings, when a nodule is classified in low or intermediate-suspicion categories, the possibility of CLT following FNA is significantly higher in the anti-TPO positive group, compared to the anti-TPO negative group.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44297570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The confocal microscopy of the cornea in post-COVID syndrome; the clinical observation","authors":"M. Lukashenko, L. Soprun, Anna Bregovskaya, N. Gavrilova, V. I. Utekhin","doi":"10.34172/ipp.2022.31338","DOIUrl":"https://doi.org/10.34172/ipp.2022.31338","url":null,"abstract":"One of the most important complications of the post-COVID-19 syndrome may be a small fiber neuropathy, which cannot be evaluated by a routine electroneuromyography. Confocal microscopy of the cornea (CCM) may be a promising method for early neuropathy verification. To study the possibility of the small fiber neuropathy evaluation using the CCM in a patient with the post-COVID-19 condition. The patient is a female of 61 years old, which suffered from a COVID-19 infection in October 2020. After a month, she noted the presence of myalgia and polyneuropathy. A year after the onset of the disease, in addition to myalgia, headaches, neuropathy pain, the patient also experienced bowel disorders and stomach aches, which cannot be explained with an alternative diagnosis. The patient underwent CCM to assess the structure of nerve fibers to prove the presence of the small fiber neuropathy as a complication of the COVID-19 infection. The presence of the small fiber neuropathy was evaluated in the patient as well as enlarged Langerhans cells in the cornea. Considering the pivotal role of these cells in the immunological processes, the \"activated\" dendritic cells in post-COVID-19 patients may serve as another evidence of the autoimmune nature of this complication and possibly, a method for the monitoring treatment effectiveness of this disease. In this clinical case of a 61-year-old patient with the post-COVID-19 syndrome, the possibility of application of the confocal microscopy of the cornea for the diagnosis of neuropathy of small fibers and monitoring of the patient›s condition is shown.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48052548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Could inadvertent intravenous injection of COVID-19 vaccines cause severe adverse events?","authors":"Z. Tajabadi, S. A. A. Safavi-Naini, B. Kwatra, A. Safavi-Naini","doi":"10.34172/ipp.2022.31392","DOIUrl":"https://doi.org/10.34172/ipp.2022.31392","url":null,"abstract":"The emergence and rapid spread of COVID-19 led to the development of novel vaccines to fight against the disease. Although COVID-19 vaccines are the major key to controlling the disease and have a good safety profile, they may be associated with some adverse events. Recently, a few studies noted that inadvertent incorrect injection of COVID-19 vaccines may lead to the development of serious adverse events. Herein, we report a case of inadvertent intravenous COVID-19 vaccine injection who developed severe anaphylactic shock following receiving the COVID-19 vaccine. Proper vaccine injection technique training may help to reduce the risk of developing adverse events and improve vaccine safety and efficacy.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43689457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}