不育男性胞浆内单精子注射(ICSI)失败后精子中胰岛素及胰岛素受体β (IR-β)的表达

IF 1.1 Q4 IMMUNOLOGY
Fateme Alizadeh, M. Hemadi, G. Saki, Akram Gavahi, F. Moramezi, A. Valizadeh
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引用次数: 0

摘要

引言:目前大多数不孕不育治疗中心都在使用精液分析来区分可生育和不育的个体。最近的研究评估了独立胰岛素和相关β受体(IR-β)在哺乳动物(如人和猪)射精精子中的表达和分泌,及其对运动参数获能和顶体反应的影响。目的:本研究旨在探讨卵浆内单精子注射(ICSI)失败男性的胰岛素、IR-β的表达以及精子和DNA断裂指数(DFI)的参数。患者和方法:本研究对15名有ICSI失败史的不明不育男性和10名有生育能力的男性进行。采集样本后,对精子参数(浓度和活力)、精子DNA片段以及胰岛素和IR-β基因的表达进行了研究。为了从SDFA试剂盒中评估DFI并回顾其表达,通过RT-PCR方法分析基因。通过t检验和协方差检验对数据进行统计学分析。结果:根据本研究的结果,有ICSI失败史的不孕男性的运动浓度和百分比显著低于有生育史的男性。此外,有ICSI失败史的不育男性的DNA断裂量显著高于有生育史的男性(P<0.05)。有ICSI成功史的不育女性精子中胰岛素表达显著低于有生育史男性(P<0.05;但两组间IR-β表达率无显著差异。结论:胰岛素基因表达的评估可作为鉴别不明原因不孕的良好标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation the expression of insulin and insulin receptor-beta (IR-β) in sperm of infertile male with failed intracytoplasmic sperm injection (ICSI)
Introduction: Most infertility treatment centers are currently using semen analysis to differentiate between fertile and infertile individuals. Recent studies have evaluated the expression and secretion of independent insulin and related beta receptor (IR-β) in mammals’ ejaculated spermatozoa such as humans and pigs, and its effect on motility parameters capacitation and acrosomal reaction. Objectives: This study aimed to investigate the expression of insulin, IR-β and the parameters of sperm and DNA fragmentation index (DFI) in men with intracytoplasmic sperm injections (ICSI) failed. Patients and Methods: This study was conducted on 15 men with unknown infertility with a history of unsuccessful ICSI and 10 fertile men. After collecting samples, sperm parameters (concentration and motility), sperm DNA fragmentation and expression of insulin and IR-β genes were investigated. To assess DFI from SDFA kit and to review the expression, the genes were analyzed via RT-PCR method. Data were analyzed statistically by t-test and covariance tests. Results: Based on the findings of this research, the concentration and percentage of motility in infertile men with a history of unsuccessful ICSI was significantly lower than fertile individuals, moreover, the amount of DNA fragmentation significantly increased in infertile men with a history of unsuccessful ICSI compared to fertile men (P < 0.05). Insulin expression in the sperm of infertile men with a failed ICSI history was significantly lower than fertile men (P < 0.05); however, the IR-β expression rate was not significantly different between the two groups. Conclusion: Evaluation of insulin gene expression can be helpful as a good marker for identifying infertility with an unknown cause.
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来源期刊
CiteScore
1.70
自引率
0.00%
发文量
65
审稿时长
3 weeks
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