Immunopathologia Persa最新文献

{"title":"The enhancement of therapeutic effects of low-intensity ultrasound with spiky and spherical gold nanoparticles on CT26 cell line; an in vitro study","authors":"Molood Gooniband Shooshtari, M. Shiran, Sakine Shirvalilou","doi":"10.34172/ipp.2024.40596","DOIUrl":"https://doi.org/10.34172/ipp.2024.40596","url":null,"abstract":"Introduction: The utilization of combination therapy has gained attention in the medical field as it can enhance treatment efficacy while minimizing side effects. Gold nanoparticles (AuNPs) have been identified as a promising candidate for enhancing the bioeffects of ultrasound (US) waves. Objectives: The primary objective of this study was to examine the impact of US irradiation when combined with spiky and spherical AuNPs on CT26 cells. Materials and Methods: Following a tailored composition, we devised and produced AuNPs with spiky and spherical morphologies. The properties of these nanoparticles were assessed using dynamic light scattering (DLS), voltammetry, and transmission electron microscopy (TEM). The CT26 cells were treated with the two types of nanoparticles and subsequently exposed to US radiation (1 W/cm2 , 1 MHz, and 10 minutes). The cytotoxic effects of various therapeutic interventions were assessed using MTT and flow cytometry assays. Results: This study’s findings indicate that nanoparticles’ morphology played a significant role in influencing the antitumor effects of US. Specifically, it was observed that the presence of spiky nanoparticles, when subjected to US, resulted in a significantly higher rate of cell death compared to spherical nanoparticles (P<0.001). Additionally, it was observed that the temperature changes induced by the spiky nanoparticles were comparatively lower. This observation implies that nanoparticles with spiky morphology enhance the non-thermal effects of US waves. Conclusion: The outcomes demonstrate a noteworthy augmentation in the biological impacts, specifically the non-thermal effects of US waves when employed in conjunction with spiky nanoparticles.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140485738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic importance of P16 in the differential diagnosis of well-differentiated liposarcoma and lipomas","authors":"P. Hedayat, M. Derakhshan, Laya Golshani, Arash Hedayat","doi":"10.34172/ipp.2023.40558","DOIUrl":"https://doi.org/10.34172/ipp.2023.40558","url":null,"abstract":"Introduction: Because of pathological overlaps, distinguishing well-differentiated liposarcoma (WDL) from lipoma is difficult. Objectives: The purpose of this study was to evaluate P16 as a differentiating marker for WDL and lipomas Materials and Methods: This cross-sectional study was performed on 38 pathology samples of lipoma and WDL in the department of pathology of Al-Zahra hospital, affiliated with Isfahan university of medical sciences, from 2011 to 2018. The studied samples were examined by immunohistochemistry (IHC) to determine the presence of P16. Results: Evaluation of IHC data showed that all of the lipoma samples (100%) had a score of 0 for IHC staining. In comparison, 84.2% of liposarcoma samples had a score of 3, and this difference was statistically significant (P<0.001). Evaluating the sensitivity and specificity of P16 gene expression showed that this marker has 84% sensitivity and 100% specificity for diagnosing liposarcoma (95% CI: 0.6042-0.9661). Conclusion: The use of p16 to differentiate WDL from lipoma has a sensitivity of 84% and a specificity of 100%, which could be very helpful in pathological practice.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140488742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare case of hydatid cyst in the chordae tendineae of papillary muscle of the right ventricle","authors":"M. Hekmat, H. Ghaderi, Zahra Ansari Aval, S. Mirjafari, R. Tirdad","doi":"10.34172/ipp.2023.40598","DOIUrl":"https://doi.org/10.34172/ipp.2023.40598","url":null,"abstract":"Hydatid cyst inside the cardiac chambers, especially the right ventricle, is a rare manifestation of the Echinococcus granulosus infection. We present a rare case of a mobile hydatid cyst on the chordae tendineae of the right ventricular papillary muscle. An 11-year-old male patient presented with multiple cysts in the body (numerous ones in the lung and liver) who had undergone left lung lobectomy due to a hydatid cyst prone to rupture. In the subsequent echocardiography, a fully mobile cyst measuring 2×1.5 cm was noticed in the right ventricle. Although the child had no cardiac presentation, due to the risk posed by its location and the high probability of sudden death or embolism, he underwent a successful cardiac and pulmonary bypass operation, and the cyst was completely excised. It was located on the chordae tendineae of the papillary muscle. In agricultural and rural countries and regions, cardiac hydatid cysts should be considered at any age. Due to the high risk of complications and mortality in cardiac hydatid cysts, regardless of the symptoms, inpatients with a positive history, cardiac examination with echocardiography must be included in diagnostic protocols. These cysts should be surgically removed as soon as possible on diagnosis, regardless of the symptoms.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140486588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sacral extradural spinal meningioma with recurrence; a case report","authors":"M. Farrokhi, Abbas Khosravifarsani, Mavlonov Jaloliddin Begijonovich","doi":"10.34172/ipp.2023.40603","DOIUrl":"https://doi.org/10.34172/ipp.2023.40603","url":null,"abstract":"Extradural spinal meningiomas are very uncommon, and just a scattering of clinical reports on this condition have been published. Despite being benign, epidural meningiomas exhibit a higher recurrence rate compared to typical meningiomas. In this case report, we described a case of sacral extradural spinal meningioma with several episodes of recurrence and discussed the clinical characteristics, radiological features, and management, along with follow-up details. A 19-year-old woman with back discomfort and lower extremity numbness. Magnetic resonance imaging (MRI) revealed a tumor on the left posterolateral S1–S2 spinal cord. L5–S3 hemilaminectomy and foraminotomy were performed. After three years, the patient had bilateral L4–S1 laminectomy and radiotherapy for tumor recurrence. Two years later, the patient presented with pain and paresthesia in the lower limbs and was diagnosed with a tumor recurrence once more. Due to sacral area adhesion from earlier surgeries and radiation, the patient received CyberKnife radiation instead of tumor resection. No complaints or signs of recurrence were detected during follow-up examinations. Extradural spinal meningiomas are uncommon and can occasionally be misidentified as malignant metastatic tumors both before and during surgery. This misclassification could potentially lead to alterations in the recommended surgical approach. Although epidural spinal meningiomas are often benign, their long-term prognosis is uncertain, and the recurrence rate is higher than ordinary meningiomas. Hence, lengthy monitoring is necessary to identify any recurrence risk (like our case).","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139530881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and comparison of the results of the Alvarado scoring system with acute inflammatory response score in the diagnosis of acute appendicitis based on the pathological evidence","authors":"M. Safaee, Reza Eshraghi Samani, Hamid Talebzadeh, Mohammad Soheil Moeini Sam","doi":"10.34172/ipp.2024.40606","DOIUrl":"https://doi.org/10.34172/ipp.2024.40606","url":null,"abstract":"Introduction: Although appendectomy is the most common reason for abdominal surgery, acute appendicitis (AA) diagnosis remained a challenging issue using various scoring systems. Objectives: The current study aims to investigate the diagnostic value of the Alvarado scoring system versus the acute inflammatory response score (AIRS) in the diagnosis of AA. Patients and Methods: The current cross-sectional study was conducted on 120 patients who underwent appendectomy between 2019 and 2020. The on-admission Alvarado and AIRS scores were evaluated for the patients. Besides, the histopathological study of the resected tissues was considered the gold standard. The receiver operating characteristics (ROC) curve was depicted for the scoring systems, and sensitivity, specificity, negative predictive value (NPV) and positive predictive value (PPV), and area under the curve (AUC) were calculated. Results: The sensitivity, specificity, NPV, and PPV of Alvarado criteria for scores >4 equaled 89.3%, 23.5%, 35.2%, and 89.3%, respectively. These amounts were calculated as 96.1%, 82.3%, 77.7%, and 97% for the AIRS, respectively. Moreover, at cut-points >8, the sensitivity of 32.1%, specificity of 100%, PPV of 100%, and NPV of 15.7% have been measured for AIRS compared with 41.7%, 88.2%, 97.1%, and 29.4% for Alvarado, respectively. The measured AUC for AIRS and Alvarado criteria accounted for 0.81 and 0.72, respectively (P value <0.05). Besides, 17 (14.16%) ones had a negative appendectomy. Conclusion: Based on the current study’s findings, both AIRS and Alvarado scoring systems were reliable means to diagnose appendicitis; however, AIRS was relatively superior considering its higher specificity and PPV in scores >8 and higher sensitivity and NPV in scores >4.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139623658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the level of agreement between bone mineral density and trabecular bone score regarding gender, age and body mass index","authors":"Alireza Rajaei, Ideh Kamkar, F. Farsad","doi":"10.34172/ipp.2022.29296","DOIUrl":"https://doi.org/10.34172/ipp.2022.29296","url":null,"abstract":"Introduction: Increasing and maintaining bone density can play a role in preventing osteoporosis, as changes in the trabecular bone score (TBS) and bone mineral density (BMD) affect bone density, especially in the spine. Objectives: The present study aimed to determine the level of agreement between TBS and BMD in patients with osteoporosis and also to investigate the relationship between these two indices with body mass index (BMI). Patients and Methods: Data were collected from 843 patients, referred to the densitometry department of Resalat hospital. BMD and TBS were measured in the subjects to determine the risk of osteoporosis. The results of BMD were measured based on T-score level. The patients’ individual and clinical characteristics were also recorded and factors influencing the prognosis of density changes were evaluated. Moreover, the effect of BMI was investigated in this study. Results: The mean age of patients was 55.5 years. The kappa coefficient and Spearman’s correlation coefficient of BMD and TBS were 0.004 and -0.015, respectively. There was a significant correlation between BMI and BMD in men. The kappa coefficient gradually increased from normal bone density to osteoporosis. There was a significant negative correlation between BMI and BMD, while a significant positive correlation between height and BMD in women was existed. On the other hand, a significant negative correlation between weight and BMD was detected accordingly. Conclusion: According to the results of our study, there is no agreement between BMD and TBS.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138600630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of gabapentin in treating overactive bladder: a quasi-experimental study","authors":"Malik Ayyad, Omar Ayaad, Hassan Alkhatatbeh, F. Sawaqed, Samer Al-Rawashdeh, Bayan Qaddumi","doi":"10.34172/ipp.2023.40574","DOIUrl":"https://doi.org/10.34172/ipp.2023.40574","url":null,"abstract":"Introduction: Overactive bladder (OAB) is a common condition in urology that affects individuals of various ages and genders, significantly impacting their quality of life. Objectives: The study aimed to examine the effectiveness of gabapentin in treating OAB in Jordan. Patients and Methods: This study was conducted at a private clinic in Jordan between 2020 and 2022. It utilized a one-group pre- and post-test design involving 50 patients. The intervention involved administering gabapentin at a daily dose of 400 mg. The effectiveness of gabapentin was evaluated using the overactive bladder symptom score (OABSS) questionnaire, administered before and after six weeks of gabapentin administration. Results: The study sample consisted of 50 patients aged between 45 and 85 years, with a mean age of 65.2 years. After the treatment, all individual variables, such as urgency, frequency, nocturia, and urgency incontinence, showed significant reductions compared to the pre-intervention values. The mean total OABSS significantly decreased from 14.6 before to 6.8 after the intervention. The paired t test yielded a result of 9.84 with a P value of less than 0.001, indicating a statistically significant improvement. Conclusion: The noteworthy improvement in OABSS scores, urgency, nocturia, frequency of micturition, and urge incontinence observed in this study suggests that gabapentin could have a role as a treatment option for OAB patients.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138602788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of viral load and autophagy-related genes polymorphisms with hepatitis B virus pre-core/core mutations in chronic hepatitis B virus Iraqi patients","authors":"Abdulhussain Kadhim Jwaziri, Maryam Esghaei, Mohammad Hadi Karbalaie Niya, Mohsen Mehrjoo, Hadi Abd Zaid Sayah, Hossein Keyvani","doi":"10.34172/ipp.2023.40575","DOIUrl":"https://doi.org/10.34172/ipp.2023.40575","url":null,"abstract":"Introduction: Chronic hepatitis B (CHB) is a global concern due to its association with cirrhosis and hepatocellular carcinoma (HCC) development. The interplay between viral load, the immune system, and host factors is critical in tumorigenesis. Autophagy is a significant contributor to immune system function, since vitamin D plays an important role in this context. Objectives: The objective of this study was to assess the association between ATG5 (rs506027 and rs510432) and ATG16L1 (rs2241880 and ATG16 rs2241879) polymorphisms, viral load, and vitamin D with HBV pre-C/C mutations in Iraqi patients with CHB. Patients and Methods: In this cross-sectional study, a total of 134 CHB patients were evaluated for ATG polymorphisms, viral load, and vitamin D levels. Blood samples were collected after obtaining ethical consent, and the mutations were analyzed using polymerase chain reaction (PCR) followed by Sanger sequencing. Serum samples from CHB patients were used for viral load and vitamin D assessment. Results: The evaluation of patients revealed that 67 (44.6%) were male and 83 (55.4%) were female, with a mean age of 36±12.7 years and a mean duration of infection of 5.2±4.8 years. Mutations in pre-C/C were observed in 20% (27/134) of the patients. There was a significant association between all evaluated ATG polymorphisms and pre-C/C mutants (P<0.05). Furthermore, there was an association between viral load and mutations in pre-C/C (P=0.03), while no statistically significant difference was found between vitamin D levels and pre-core/core mutants or viral load. Conclusion: Our study demonstrates a higher frequency of ATG5 (rs506027 and rs510432) and ATG16L1 (rs2241880 and ATG16 rs2241879) polymorphisms, as well as a higher viral load in Iraqi CHB patients with HBV pre-C/C mutations.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135959225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of analgesics on the risk of ovarian cancer; a systematic review and meta-analysis of cohort and case-control studies","authors":"Javad Sadeghi, Saba Bazzazi, Farinaz Fattahi, Moloud Alsadat Mousavi, Ardeshir Tajbakhsh, Sadaf Rassouli, Siavash Sangi, Mahshad Ghezelbash, Anna Ghorbani","doi":"10.34172/ipp.2023.40572","DOIUrl":"https://doi.org/10.34172/ipp.2023.40572","url":null,"abstract":"Introduction: Ovarian cancer is the sixth leading cancer-related mortality cause in women worldwide. Analgesics may hinder the occurrence of ovarian cancer through inflammation relief. The present study seeks to examine the relationship between analgesic consumption and ovarian cancer risk through a systematic review and meta-analysis. Materials and Methods: PubMed, Scopus, Web of Science, Cochrane databases, and Google Scholar search engines were searched for works published by May 2023 using standard keywords to collect the required data sources. Acquired data were then analyzed in STATA version14, considering a significance level of P < 0.05 in statistical tests. Results: The present meta-analysis comprised 21 studies (14 case-control and 7 cohort studies) performed on 53,755 subjects. Results indicated that taking aspirin reduced the risk of ovarian cancer by 8% (OR: 0.92; 95% CI: 0.87, 0.98), whereas other non-aspirin NSAIDs (non-steroidal anti-inflammatory drugs) did not significantly affect the ovarian cancer risk (OR: 0.91; 95% CI: 0.81, 1.02). Similar results were observed in the case of ibuprofen and acetaminophen, with non-significant relationships between the risk of ovarian cancer and with the consumption of both acetaminophen (OR: 0.95; 95% CI: 0.84, 1.08) and ibuprofen (OR: 0.76; 95% CI: 0.50, 1.14). Furthermore, the duration of analgesic consumption was not significantly linked to the risk of ovarian cancer in the case of all studied drugs. Conclusion: Among aspirin, ibuprofen, acetaminophen, and non-aspirin NSAIDs, only aspirin was found to reduce the risk of ovarian cancer in women while the other studied drugs did not influence the studied risk. However, further research is recommended to confirm the results. Registration: This study has been compiled based on the PRISMA checklist, and its protocol was registered on the PROSPERO (CRD42023434730) and Research Registry (UIN: reviewregistry1668) websites.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44794728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of COVID-19 on renal transplant recipients","authors":"Ganesan Geethanjali, E. Ramprasad, Sekar Manikantan, M. Jayakumar","doi":"10.34172/ipp.2023.39489","DOIUrl":"https://doi.org/10.34172/ipp.2023.39489","url":null,"abstract":"Introduction: It was essential to explore immunosuppressant management strategies and potential clinical variables associated with COVID-19 related mortality in order to provide insight for clinicians attempting to manage kidney transplant recipients during the ongoing severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) pandemic. Objectives: The aim of the study was to assess the impact of COVID-19 on post-transplant renal function and outcome of immunosuppressant management on COVID-19. Patients and Methods: This is a cross sectional observational study conducted from March 2020 to January 2022 in a tertiary care hospital in South India. Baseline characteristics, comorbidities, history of graft dysfunction, symptoms and immunosuppressant modification was noted. Outcomes of COVID-19 such as acute kidney injury (AKI), need for dialysis and post COVID-19 complications were noted. The statistics were expressed as percentage for categorical variables and mean ± SD for continuous variables. Results: Out of 400 renal transplant patients on regular follow up, 28 patients developed COVID-19. The incidence of AKI was 64.2%. Immunosuppressant dose modification was done in majority of patients [mycophenolate mofetil (28.5%), steroids (53.5%) and tacrolimus (39.2%)]. Outcomes included recovery from AKI in 61.1%, recovery from oxygen dependence in 100% patients with an overall mortality rate of 7.1% patients. About 17.8% patients developed post-COVID-19 complications. Conclusion: Immunosuppressant dose modification during COVID-19 could play a role in development of AKI; infection being an independent risk factor. Patients should be monitored for development of post-COVID-19 complications.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42763119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}