表型方法与BD Phoenix M50自动微生物系统检测伊朗伊斯法罕血液中非发酵革兰氏阴性菌属的比较研究

IF 1.1 Q4 IMMUNOLOGY
Shahnaz Eskandari, E. Amini, A. Baradaran, S. Mobasherizadeh, S. Rostami, M. Derakhshan
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引用次数: 0

摘要

前言:采用表型法和Phoenix法对血液培养样品中分离的非发酵革兰阴性杆菌属和种进行了比较研究。目的:本研究旨在将Phoenix系统与目前可用的表型过程进行比较。材料和方法:这项描述性分析横断面研究评估了2019年至2020年从AL Zahra和Kashani医院需要血液培养的患者身上采集的30份样本。将标本注射到BACTEC中™. 通过表型法鉴定为非发酵革兰氏阴性菌的阳性培养物被纳入研究,然后使用Phoenix法进行评估,以确定非发酵革兰氏阳性菌的属和种。然后进行了比较。结果:本研究鉴定出30株非发酵革兰氏阴性菌。Phoenix方法显示78.5%的诊断为不动杆菌,而表型方法确定86.7%为不动菌。结论:本研究表明Phoenix和表型方法在鉴定细菌类型方面存在显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The comparative survey of phenotypic methods and the BD Phoenix M50 automated microbiology system for detecting the genus of non-fermenting gram-negative bacteria isolated from blood samples in Isfahan, Iran
Introduction: A comparative study was conducted to identify the genus and species of non-fermenting gram-negative bacilli isolated from blood culture samples using phenotypic methods and the Phoenix method. Objectives: This study aimed to evaluate the Phoenix system compared to the currently available phenotypic process. Materials and Methods: This descriptive-analytical cross-sectional study evaluated 30 samples collected from 2019 to 2020 from patients at AL Zahra and Kashani hospitals who required blood cultures. The specimens were injected into BACTEC™. Positive cultures identified as non-fermenting gram-negative bacteria by the phenotypic method were included in the study and then evaluated using the Phoenix method to determine the genus and species of non-fermenting gram-negative bacteria. A comparison was then conducted. Results: The study identified 30 non-fermenting gram-negative bacteria. The Phoenix method revealed that 78.5% of the diagnoses were Acinetobacter, while the phenotypic approach identified 86.7% as Acinetobacter spp. Conclusion: The present study demonstrated a significant difference between the Phoenix and phenotypic methods in identifying the type of bacteria.
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来源期刊
CiteScore
1.70
自引率
0.00%
发文量
65
审稿时长
3 weeks
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