Immunological communications最新文献_第8页

Comparison of cultures of human lymphocytes obtained following NH4Cl induced red blood cell lysis and Ficoll-Hypaque density gradient centrifugation. NH4Cl诱导红细胞溶解和Ficoll-Hypaque密度梯度离心后人淋巴细胞培养物的比较。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050752

M R Simon, L D Roi, S Desai, D J Salberg, N R Rose

{"title":"Comparison of cultures of human lymphocytes obtained following NH4Cl induced red blood cell lysis and Ficoll-Hypaque density gradient centrifugation.","authors":"M R Simon, L D Roi, S Desai, D J Salberg, N R Rose","doi":"10.3109/08820138309050752","DOIUrl":"https://doi.org/10.3109/08820138309050752","url":null,"abstract":"Lymphocyte thymidine uptake in leucocytes prepared by NH4Cl induced RBC lysis was compared with that of lymphocytes obtained following Ficoll-Hypaque centrifugation using polynomial regression dose response curves which adjusted for inherent subject variability. Eleven male subjects were studied. Heparinized blood was either layered in Ficoll-Hypaque or treated with 0.87% NH4Cl to induce RBC lysis. Phytohemagglutinin-P (PHA) dose response curves were determined for each subject using triplicate 5 day cultures. For each subject, at each Pha concentration ranging from 12.5 to 167micrograms/ml, triplicate mean cpm of Ficoll-Hypaque treated lymphocytes and NH4Cl treated lymphocytes were compared by the 2 tailed Student's T test. Responses of cultures of NH4Cl treated lymphocytes were significantly different (less than 0.05) from the responses of cultures of Ficoll-Hypaque treated lymphocytes for at least 2 PHA doses in 8 of the 11 subjects. The quadratic dose response curves were also significantly different (p less than 0.05) for 9 of the 11 subjects. A repeated measures quadratic regression was used to determine the best fit dose response curves for each of the experimental conditions. For Ficoll-Hypaque treated lymphocytes, mean cmp=44,977+1400.4 (PHA concentration)-5.90 (PHA concentration)2+ subject effect; s.e.=22,256; for NH4Cl treated lymphocytes, mean cpm=58,039+1022.8(Pha concentration)-5.32 (PHA concentration)2+ subject effect; s.e.=37,966. The p values from the F tests for significance of these quadratic regressions are different, F=1.91, d.f.=30,30; p less than 0.001. The lack of significance of the NH4Cl treated lymphocytes' does response quadratic regression and its larger s.e. indicates that the NH4Cl treated lymphocyte culture dose response curve is less predictable than that of Ficoll-Hypaque treated lymphocytes.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 3","pages":"301-14"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309050752","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17660084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Nonenzymatic glycosylation of human IgG: in vitro preparation. 人IgG非酶糖基化的体外制备。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309066868

M A Cohenford, J C Urbanowski, D C Shepard, J A Dain

引用次数: 11

Molecular localization of the full profile of the continuous regions recognized by myoglobin-primed T-cells using synthetic overlapping peptides encompassing the entire molecule. 利用合成的覆盖整个分子的重叠肽，对肌红蛋白引发的t细胞识别的连续区域的完整轮廓进行分子定位。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309025440

G S Bixler, M Z Atassi

{"title":"Molecular localization of the full profile of the continuous regions recognized by myoglobin-primed T-cells using synthetic overlapping peptides encompassing the entire molecule.","authors":"G S Bixler, M Z Atassi","doi":"10.3109/08820138309025440","DOIUrl":"https://doi.org/10.3109/08820138309025440","url":null,"abstract":"The molecular localization of the full antigenic profile for T-cell recognition of a complex multi-determinant protein antigen has not to date been accomplished. Previously, this laboratory has introduced a comprehensive strategy for the systematic localization of all continuous antigenic sites within a protein. This strategy depends on the synthesis of a series of overlapping peptides that together account for the entire structure of a protein. Such a strategy has been applied, in this report, to the delineation of the continuous sites of T-cell recognition of sperm whale myoglobin. Thirteen peptides, accounting for the entire protein chain, were synthesized and subsequently examined in vitro for their ability to stimulate lymph node cells from myoglobin primed DBA/2 (H-2d) mice, a known high responder. This strategy has enabled for the first time the localization of the full profile of the protein regions which contain the sites of T-cell recognition. Three regions gave a high response (one being immunodominant and coinciding with antigenic, i.e. antibody binding, site 4 of myoglobin). At least three regions appear to coincide with previously known antigenic (antibody binding) sites. Noteworthy is the finding of regions that are recognized by T-cells but to which no detectable antibody response is directed.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 6","pages":"593-603"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309025440","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17266275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Stimulation of the Na/K pump in LK sheep erythrocytes by immunoglobulin fragments. 免疫球蛋白片段对LK羊红细胞钠钾泵的刺激作用。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309025438

R L Bratcher, C L Kanik-Ennulat, P J Logue, P B Dunham

引用次数: 3

Comparison between dissociation and inhibition of association of DNA/anti-DNA complexes. 脱氧核糖核酸/抗脱氧核糖核酸复合物解离与抑制结合的比较。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309066867

R J Smeenk, L A Aarden, C J van Oss

引用次数: 27

The effectiveness of 3H-uridine and 3H-leucine for labeling of T and B cells of rats and mice. 3h -尿苷和3h -亮氨酸对大鼠和小鼠T细胞和B细胞的标记效果。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050754

N van Rooijen, L Hofland, C D Dijkstra

引用次数: 0

Perfusion of canine serum over Staphylococcus aureus Cowan 1: Evidence for release of protein A and changes in specific antibody activity. 犬血清在金黄色葡萄球菌上灌注:蛋白A释放和特异性抗体活性变化的证据。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309025439

J Balint, F R Jones

{"title":"Perfusion of canine serum over Staphylococcus aureus Cowan 1: Evidence for release of protein A and changes in specific antibody activity.","authors":"J Balint, F R Jones","doi":"10.3109/08820138309025439","DOIUrl":"https://doi.org/10.3109/08820138309025439","url":null,"abstract":"Sera from three normal dogs were assessed for levels of Clq binding IgG and complement consumption after perfusion over Staphylococcus aureus Cowan I (SAC). Increased levels of Clq binding IgG were detected after perfusion of sera over SAC and were associated with complement consumption. Canine antiserum to human erythrocytes were also perfused over SAC and assessed for Clq binding IgG and hemolytic activity. Increased levels of Clq binding IgG in post-perfusion samples were detected which were associated with a decrease in hemolytic activity. IgG was determined to be present in molecular weight fractions greater than 200,000 M.W. in post-perfusion chromatographically fractionated sera. Moreover, 5% polyethylene glycol (PEG) precipitated IgG from post-perfusion sera was functional in antibody dependent cellular cytotoxicity assays. Putative staphylococcal protein A isolated from post-perfusion sera produced a precipitin band in double diffusion agarose gel studies when reacted with normal human and canine sera. A polypeptide co-migrating with purified protein A could be detected by polyacrylamide gel electrophoresis (PAGE) analysis of the post-perfusion isolated protein A. Addition of purified protein A to canine antiserum resulted in decreased hemolytic activity of the serum which was associated with increased levels of Clq binding IgG.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 6","pages":"573-91"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309025439","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17664395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Measurement of myelin basic protein and of anti-basic protein antibodies by ELISA utilizing biotinylated antibodies. 利用生物素化抗体，ELISA法测定髓鞘碱性蛋白和抗碱性蛋白抗体。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309060855

L Spatz, L Whitman, M J Messito, G Nilaver, S Ginsberg, N Latov

引用次数: 12

Inhibition of blastogenesis by native and polyethylene glycol-modified asparaginases from Escherichia coli and Vibrio succinogenes. 大肠杆菌和琥珀酸弧菌天然和聚乙二醇修饰的天冬酰胺酶对胚胎发生的抑制作用。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050749

A Bendich, D Kafkewitz, A Abuchowski, F F Davis

引用次数: 2

Monoclonal antibody analysis of T-lymphocyte subsets in young and aged adults. 青年人和老年人t淋巴细胞亚群的单克隆抗体分析。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309066871

J E Nagel, F J Chrest, R S Pyle, W H Adler

{"title":"Monoclonal antibody analysis of T-lymphocyte subsets in young and aged adults.","authors":"J E Nagel, F J Chrest, R S Pyle, W H Adler","doi":"10.3109/08820138309066871","DOIUrl":"https://doi.org/10.3109/08820138309066871","url":null,"abstract":"Eleven monoclonal antibodies identifying surface antigens present on human T lymphocytes were utilized to investigate the effects of advanced age on peripheral blood lymphocyte subsets. Both the proportion and number of lymphocytes recognized by five antibodies reactive with 'pan' T cell antigens (OKT3, OKT11, Leu4, T101 and Lyt3) decreased with age. The percentage of helper/inducer (OKT4+, Leu3a+) cells remained constant; however the proportion of Leu2a+, suppressor/cytotoxic cells declined. There was no change with age in the percent representation of OKT9+ or OKT10+ cells, nor in the ratio of helper/inducer to suppressor/cytotoxic cells (OKT4+/OKT8+ or Leu3a+/Leu2a+). Absolute numbers of helper/inducer (OKT4+, Leu3a+), suppressor/cytotoxic (OKT8+, Leu2a+), OKT9+ and OKT10+ cells were lower in elderly individuals as the result of lymphocytes constituting a lower percentage of the peripheral blood white cell population in this age group. While only small differences existed between the lymphocyte populations of young and aged men; aged women, compared to young women, had more dramatic shifts in their T cell populations. Comparison of antibodies putatively identifying similar (the same) functional groups of T cells demonstrated excellent correlation between the percentage of cells enumerated with the antibodies OKT3+: Leu4+ (r = .951), OKT4+: Leu3a+ (r = .914), OKT8+: Leu2a+ (r = .896), and in the ratio of helper/inducer to cytotoxic/suppressor OKT4+/OKT8+: Leu3a+/Leu2a+ (r = .926) cells.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 2","pages":"223-237"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309066871","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17660011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 51