Immunological communications最新文献_第10页

Monoclonal antibodies and a heterobifunctional reagent: a novel approach to the vectorial labeling of selected membrane proteins. 单克隆抗体和异功能试剂:一种新的膜蛋白载体标记方法。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050735

Y H Jou, B S Schepart, R B Bankert

{"title":"Monoclonal antibodies and a heterobifunctional reagent: a novel approach to the vectorial labeling of selected membrane proteins.","authors":"Y H Jou, B S Schepart, R B Bankert","doi":"10.3109/08820138209050735","DOIUrl":"https://doi.org/10.3109/08820138209050735","url":null,"abstract":"Many applications exist and others are envisioned for the chemical coupling of macromolecules to membrane proteins on the surface of mammalian cells. The ability to use antibody as a means to label and subsequently to follow the distribution of cell surface proteins is reported here. A new procedure is outlined for covalently coupling monoclonal antibodies to thiol-containing membrane proteins. The key reagent in the coupling reaction is the commercially available heterobifunctional reagent N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP). The coupling proceeds in a simple two-step reaction in aqueous medium under very mild conditions. This results in a very efficient and stable attachment of anti-hapten antibodies to a selected set of cell surface proteins without any loss in cell viability and without denaturing the antibody molecule. The hapten-binding activity of the antibody is exploited to monitor the re-distribution of the antibody-labeled cell surface proteins periodically after the coupling reaction. The hapten binding activity can also be utilized to isolate membrane macromolecules via affinity chromatography.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"11 5","pages":"357-75"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138209050735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18168091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Macrophage and lymphocyte contributions in resistance of Candida albicans infections. 巨噬细胞和淋巴细胞在抵抗白色念珠菌感染中的作用。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209094131

T L Hadfield, S Marcus

引用次数: 10

Affinity diffusion. I. Method for measuring dissociation constants of precipitating antibodies. 亲和力扩散。1 .测定沉淀抗体解离常数的方法。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209057749

C J van Oss, P M Bronson, D R Absolom

引用次数: 6

Absence of circulating IgG immune Complexes in C57Bl/6 mice with age-associated renal IgG deposits. 年龄相关性肾IgG沉积的C57Bl/6小鼠循环IgG免疫复合物缺失

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209057745

P K Poskitt, T R Poskitt

引用次数: 1

The effect of Pseudomonas aeruginosa on the immunogenicity of enterobacterial common antigen. 铜绿假单胞菌对肠杆菌共同抗原免疫原性的影响。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050745

S Ramia, D Amsterdam, H Mayer, E Neter

引用次数: 3

Inhibition of Con A mitogenesis by serum from procainamide-treated patients and patients with systemic lupus erythematosus. 普鲁卡因胺治疗患者和系统性红斑狼疮患者血清对Con A有丝分裂发生的抑制作用。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050722

R H Tannen, S Cunningham-Rundles

引用次数: 6

Application of cultured human myeloid cells (K562) for detection of immune complexes in human sera. 培养的人髓细胞(K562)在检测人血清免疫复合物中的应用。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050738

R K Gupta, J F Huth, S H Golub

{"title":"Application of cultured human myeloid cells (K562) for detection of immune complexes in human sera.","authors":"R K Gupta, J F Huth, S H Golub","doi":"10.3109/08820138209050738","DOIUrl":"https://doi.org/10.3109/08820138209050738","url":null,"abstract":"Cultured human myeloid cells (K562) are known to bear Fc receptors that bind with aggregated human IgG (AHG). These cells were used to develop a radiometric assay for detection and quantitation of immune complexes (IC) in human sera. The binding of AHG or in vitro-formed IC between keyhole lympet hemocyanin (KLH) and human anti-KLH to the K562 cells did not require complement. When the K562 radiometric assay was compared to the complement-consumption assay, the K562 radiometric assay could detect IC over a wider range of antibody:antigen ratios. The incidence and mean IC values detected by the K562 radiometric assay in sera from cancer patients and patients with connective tissue diseases (498 +/- 445 and 436 +/- 209 micrograms AHG equ/ml, respectively) were significantly higher than in sera from healthy volunteers (107 +/- 62 micrograms AHG equ/ml). The IC level among sera from cancer patients ranged from 1-3200 micrograms AHG equ/ml. Studies with a limited number of sera from melanoma and sarcoma patients revealed that the mean IC values were significantly higher in patients who had clinically detectable disease than in those with no evidence of disease. Since the K562 cells do not require complement to interact with AHG, the K562 radiometric assay may be potentially useful for detecting IC in pathologic sera which may or may not contain in vivo-bound complement components.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"11 5","pages":"401-19"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138209050738","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18032619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A new approach for the immunogenic presentation of membrane-bound human colon tumor antigens. 膜结合人结肠肿瘤抗原免疫原性呈递的新方法。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050731

B H Tom, T J Goodwin, J Sengupta, B D Kahan, L P Rutzky

引用次数: 7

Selective excretion of IgA in rat bronchial secretions: lack of significant contribution from plasma IgA. 大鼠支气管分泌物中IgA的选择性排泄:血浆IgA缺乏显著贡献。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050741

I Lemaître-Coelho, M Yamakido, P C Montgomery, A E Langendries, J P Vaerman

{"title":"Selective excretion of IgA in rat bronchial secretions: lack of significant contribution from plasma IgA.","authors":"I Lemaître-Coelho, M Yamakido, P C Montgomery, A E Langendries, J P Vaerman","doi":"10.3109/08820138209050741","DOIUrl":"https://doi.org/10.3109/08820138209050741","url":null,"abstract":"Concentrated rat bronchial washings (BW) were analyzed by gel-filtration and immunochemical methods. BW contained mainly albumin, transferrin and IgG. Free secretory component and secretory IgA were identified in BW; the BW-IgA had the same three sedimentation coefficients, i.e. +/- 11 S, 13 S, 15 S by sucrose density gradient ultracentrifugation, as rat milk and rat bile IgA; the three peaks were secretory IgA. Compared to serum, and relatively to albumin, BW were significantly enriched in IgA, although much less than rat bile. Purified polyclonal rat polymeric 125I-IgA was injected intravenously into normal rats, and into rats with bile duct ligation or partial hepatectomy, which decrease the liver plasma-to-bile transfer of IgA. BW were then collected, one or four hours later, to assess the recovery of the 125I-IgA in BW and to estimate the contribution of serum IgA to BW-IgA. Very little 125I-IgA (less than 0.2%) was recovered in all BW. The specific activity, measured only in the rat with the highest recovery in BW, was 20 times lower in BW than in serum. The data demonstrate that rat serum IgA does not contribute significantly to IgA in BW.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"11 6","pages":"441-53"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138209050741","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18184650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Helix pomatia receptors on rat T lymphocytes and bone marrow cells. 大鼠T淋巴细胞和骨髓细胞上的螺旋鱼受体。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050729

J A Killen, J H Holda, R H Swanborg

引用次数: 1