Immunological communications最新文献_第9页

Luminescence energy transfer studies of C-reactive protein. Binding of terbium (III) ions in C-reactive protein. c反应蛋白的发光能量转移研究。c反应蛋白中铽(III)离子的结合。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050751

M T Short, A P Osmand

{"title":"Luminescence energy transfer studies of C-reactive protein. Binding of terbium (III) ions in C-reactive protein.","authors":"M T Short, A P Osmand","doi":"10.3109/08820138309050751","DOIUrl":"https://doi.org/10.3109/08820138309050751","url":null,"abstract":"The acute phase of inflammation is characterized by numerous changes in blood composition, perhaps the most dramatic of these being the elevation of C-reactive protein levels. C-reactive protein (CRP) is known to bind to molecules containing phosphocholine-substituents following reaction with Ca2+ ions. Luminescence energy transfer (LET) has been used effectively to study the Ca2+ and Mg2+ binding properties of many proteins by employing appropriate lanthanides (III). We have used Tb3+ as an isomorphous analogue to study Ca2+ binding to CRP. Energy transfer occurs effectively and demonstrates the importance of aromatic residues (viz., tyrosine and tryptophan) in the binding of Tb3+. The binding of Tb3+ is remarkably dependent on the pH and indicates the requirement of a deprotonated residue in the pH range 6.4 +/- 0.2 for effective Tb3+ binding. A 50-fold molar excess of Ca2+ is sufficient to displace the Tb3+ suggesting that Tb3+ is bound with greater affinity to CRP than the natural analogue Ca2+. We propose that Tb3+ (by inference Ca2+) binding takes place near the CRP subunit disulfide bond, where two histidine residues are present. The pH dependency of Tb3+ binding is best explained by the deprotonation of a histidine residue(s) in CRP.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 3","pages":"291-300"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309050751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17936112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Antibodies with preselected specificities to protein regions evoked by immunization with free synthetic peptides: dose response to myoglobin antigenic sites reveal an optimum dose for each antigenic site. 抗体预先选择特异性的蛋白质区域诱发免疫与自由合成肽:剂量反应肌红蛋白抗原位点揭示了每个抗原位点的最佳剂量。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050762

C R Young, H E Schmitz, M Z Atassi

{"title":"Antibodies with preselected specificities to protein regions evoked by immunization with free synthetic peptides: dose response to myoglobin antigenic sites reveal an optimum dose for each antigenic site.","authors":"C R Young, H E Schmitz, M Z Atassi","doi":"10.3109/08820138309050762","DOIUrl":"https://doi.org/10.3109/08820138309050762","url":null,"abstract":"Previously, we have shown by radioimmune antibody binding studies that immunization with each of the five synthetic antigenic sites of sperm-whale myoglobin (Mb), in their free form (i.e. not coupled to a carrier), resulted in the formation of antibodies that bound specifically to Mb and exclusively to the peptide that was used in immunization. In the present series of studies we have immunized separate groups of Balb/cByJ mice with different amounts of each of the free synthetic antigenic sites so as to determine the optimum immunizing dose for eliciting serum antibodies that bind specifically to Mb. The synthetic peptides corresponded to: Site 1 (peptide 15-22), Site 2 (peptide 56-62), Site 3 (peptide 94-100), Site 4 (peptide 113-120, and Site 5 (peptide 145-151). The dose range of each of the antigenic sites used in immunization was from 6 micrograms to 100 micrograms. Radioimmune antibody binding studies indicated that there is an optimal immunizing dose for each of the five antigenic sites which was smaller than anticipated. The significance of these findings is discussed.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 4","pages":"419-28"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309050762","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17260219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Identification of cytosolic antigens from GW-39 adenocarcinoma cells by crossed immunoelectrophoresis and immunofluorescence. 交叉免疫电泳和免疫荧光技术鉴定GW-39腺癌细胞细胞质抗原。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050753

C W Taylor, S Chakrabarty, K S Schauder, L C Yeoman

{"title":"Identification of cytosolic antigens from GW-39 adenocarcinoma cells by crossed immunoelectrophoresis and immunofluorescence.","authors":"C W Taylor, S Chakrabarty, K S Schauder, L C Yeoman","doi":"10.3109/08820138309050753","DOIUrl":"https://doi.org/10.3109/08820138309050753","url":null,"abstract":"Rabbits were immunized with a cytosolic fraction prepared from human adenocarcinoma cells of the colon (GW-39). The antibodies obtained were analyzed using crossed immunoelectrophoresis and were found to precipitate 23 distinct antigens in the cytosolic fraction from colon tumor cells. After preabsorption with human serum and plasma as well as acetone powders prepared from 2 normal human tissues and 4 normal hamster tissues, 1 major immunodominant cytosol antigen (CA-3) and two less intense immunoprecipitin peaks (CA-1 and CA-5) remained detectable by the crossed immunoelectrophoretic method. The preabsorptions with normal tissues were sufficiently complete to remove anti-carcinoembryonic antigen antibodies and showed that antigens CA-1, CA-3 and CA-5 are immunologically distinct from carcinoembryonic antigen. Indirect immunofluorescence localization studies with preabsorbed anti-cytosol antibodies and FITC-conjugated second antibody showed that these antigens were expressed in the cytoplasm and at the cell surface in several human colon tumor cell lines, their subclones and in primary colon tumor specimens.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 3","pages":"315-29"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309050753","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17409895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Modulation by zinc of the in vitro antibody response to T-dependent and T-independent antigens. 锌对体外抗体对t依赖性和t非依赖性抗原反应的调节。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050760

I Malavé, S Claverie-Benureau, I R Benaím

引用次数: 16

Defective mononuclear phagocytic function in mice homozygous for the cribriform degeneration autosomic recessive mutation. 小鼠单核吞噬功能缺陷纯合子为筛状变性常染色体隐性突变。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050758

M C Cerquetti, D O Sordelli, O H Pivetta

引用次数: 0

Hypothyroidism and antibody production in immature male chickens. 未成熟雄鸡甲状腺功能减退和抗体产生。

Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309025437

M M Mashaly, S L Youtz, R F Wideman

{"title":"Hypothyroidism and antibody production in immature male chickens.","authors":"M M Mashaly, S L Youtz, R F Wideman","doi":"10.3109/08820138309025437","DOIUrl":"https://doi.org/10.3109/08820138309025437","url":null,"abstract":"This study was conducted to determine if hypothyroidism has an effect on humoral immunity in immature male chickens. Two week old Single Comb White Leghorn male chicks were used as experimental animals. Two experiments were conducted using different methods to induce hypothyroidism. In Experiment 1, birds were surgically thyroidectomized (Tx group) and in Experiment 2, hypothyroidism was induced by supplementing the feed throughout the experiment with 0.1% propylthiouracil (PTU group). Antibody production against sheep red blood cells (SRBC) (thymus-dependent antigen) and Brucella abortus (BA) (thymus-independent antigen) was tested at 4 weeks of age. Serum concentrations of T4 and T3 were measured in birds from each treatment group at 5 and 9 weeks of age. Body weights were recorded and birds were then autopsied and thyroid gland weights were measured. Hypothyroidism was successfully induced in both Tx and PTU birds, as reflected by significant reduction in body weights in both groups, enlargement of thyroid glands in PTU birds and absence of thyroid glands in Tx birds. Though T4 and T3 were reduced in sera of treated birds, considerable amounts of these hormones were detected. Hypothyroidism did not seem to have profound or consistent effects on antibody production against SRBC or BA. The possibility that thyroid hormones play a role in antibody production was not ruled out. However, it was suggested that within the physiological range of thyroid gland activity, thyroid hormones may not significantly regulate antibody production.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 6","pages":"551-63"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309025437","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17728911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Suppression of experimental allergic encephalomyelitis by interferon. 干扰素抑制实验性变应性脑脊髓炎的作用。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050718

S L Abreu

引用次数: 79

Reactivity of antiserum to human brain with peripheral blood lymphocytes. 抗血清对人外周血淋巴细胞的反应性。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050730

V Wicher, C Amesty

引用次数: 1

Cyclic AMP-dependent protein kinase content of murine splenic T and B lymphocytes and non-lymphoid tissues. 小鼠脾T淋巴细胞和B淋巴细胞及非淋巴组织中环amp依赖性蛋白激酶的含量。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209094133

R A Ortez

引用次数: 1

Alloantigen-mediated adherence of rat hepatocytes to antibody-coated polystyrene dishes. 同种异体抗原介导的大鼠肝细胞粘附在抗体包被的聚苯乙烯培养皿上。

Immunological communications Pub Date : 1982-01-01 DOI: 10.3109/08820138209050736

J M Hunt, M T Buckley, B A Laishes

引用次数: 4