{"title":"3h -尿苷和3h -亮氨酸对大鼠和小鼠T细胞和B细胞的标记效果。","authors":"N van Rooijen, L Hofland, C D Dijkstra","doi":"10.3109/08820138309050754","DOIUrl":null,"url":null,"abstract":"<p><p>Using a technique for combined autoradiography and immunoperoxidase staining, we studied the incorporation of 3H-Uridine and 3H-Leucine into T and B lymphocytes of rats and mice. While in mouse both T and B cells were clearly labeled by incorporation of 3H-Uridine, a portion of rat T cells remained unlabeled. Furthermore, the majority of rat B cells was not labeled. In contrast, mouse T and B cells as well as rat T and B cells could be labeled with 3H-Leucine to a comparable degree.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 3","pages":"331-5"},"PeriodicalIF":0.0000,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309050754","citationCount":"0","resultStr":"{\"title\":\"The effectiveness of 3H-uridine and 3H-leucine for labeling of T and B cells of rats and mice.\",\"authors\":\"N van Rooijen, L Hofland, C D Dijkstra\",\"doi\":\"10.3109/08820138309050754\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Using a technique for combined autoradiography and immunoperoxidase staining, we studied the incorporation of 3H-Uridine and 3H-Leucine into T and B lymphocytes of rats and mice. While in mouse both T and B cells were clearly labeled by incorporation of 3H-Uridine, a portion of rat T cells remained unlabeled. Furthermore, the majority of rat B cells was not labeled. In contrast, mouse T and B cells as well as rat T and B cells could be labeled with 3H-Leucine to a comparable degree.</p>\",\"PeriodicalId\":13417,\"journal\":{\"name\":\"Immunological communications\",\"volume\":\"12 3\",\"pages\":\"331-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/08820138309050754\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunological communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/08820138309050754\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08820138309050754","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The effectiveness of 3H-uridine and 3H-leucine for labeling of T and B cells of rats and mice.
Using a technique for combined autoradiography and immunoperoxidase staining, we studied the incorporation of 3H-Uridine and 3H-Leucine into T and B lymphocytes of rats and mice. While in mouse both T and B cells were clearly labeled by incorporation of 3H-Uridine, a portion of rat T cells remained unlabeled. Furthermore, the majority of rat B cells was not labeled. In contrast, mouse T and B cells as well as rat T and B cells could be labeled with 3H-Leucine to a comparable degree.
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