Immunological communications最新文献_第5页

Use of human IgM derived fragments to study structures responsible for protein A-reactivity. 利用人类IgM衍生片段研究负责蛋白a反应性的结构。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409033888

M A Vidal, F P Conde

引用次数: 3

The effect of alpha 2HS glycoprotein on lymphocyte reactivity to phytohemagglutinin. α 2HS糖蛋白对淋巴细胞对植物血凝素反应性的影响。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409025448

J G Lewis, C M André

引用次数: 3

Presence of a specific immune complex in the cell-free ascites of a spontaneously metastasizing rat mammary adenocarcinoma, TMT-081. 自发性转移性大鼠乳腺腺癌TMT-081无细胞腹水中特异性免疫复合物的存在

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409025450

S K Ghosh, O A Roholt

引用次数: 0

Serological interactions among sera of human renal graft recipients. 人肾移植受者血清的血清学相互作用。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409025460

T Morito, K Kano, F Milgrom

引用次数: 1

T cell recognition of lysozyme. II. Shift in specificity during long-term culture determined by synthetic overlapping peptides comprising the entire protein chain. T细胞识别溶菌酶。2在长期培养过程中，特异性的转变由合成重叠肽组成的整个蛋白质链决定。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409025459

G S Bixler, T Yoshida, M Z Atassi

{"title":"T cell recognition of lysozyme. II. Shift in specificity during long-term culture determined by synthetic overlapping peptides comprising the entire protein chain.","authors":"G S Bixler, T Yoshida, M Z Atassi","doi":"10.3109/08820138409025459","DOIUrl":"https://doi.org/10.3109/08820138409025459","url":null,"abstract":"Recently, by using a comprehensive synthetic strategy developed in this laboratory, we localized four sites (T sites) within the polypeptide chain of lysozyme recognized by T cells from two high responder mouse strains, DBA/1 and B10.BR. However, to detect minor specificities, the selective enrichment of lysozyme reactive cells, would be required. T cells from long-term cultures maintained by repeated stimulation with antigen are selectively enriched for that antigen. It is not known whether maintaining T cells for extended periods of time in vitro has any consequences on the profile of T cell recognition. In the present study, T cells from long-term cultures, derived from these two high responder lysozyme-primed mouse strains, were examined for their responsiveness to a series of synthetic overlapping peptides encompassing the entire polypeptide chain of the lysozyme molecule. We have found that the profile of T cell recognition of the long-term cultures may not reflect that of the lysozyme primed lymph node cells, but that it is subject to a shift in specificity towards submolecular features of the molecule. In addition, we have identified in B10.BR mice another region within the polypeptide chain of lysozyme (residues 72-84) which may potentially harbor a previously undetected (in lymph node cells) minor T site.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"13 2","pages":"161-72"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138409025459","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17268843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Comparison of various preparations of nuclear antigens by hemagglutination inhibition (HAI). 不同核抗原制备方法的血凝抑制(HAI)比较。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409025456

A M Boak, L A Kincaid, E L Treadwell, P McDonald, K R Ellis, G C Sharp, P F Agris

{"title":"Comparison of various preparations of nuclear antigens by hemagglutination inhibition (HAI).","authors":"A M Boak, L A Kincaid, E L Treadwell, P McDonald, K R Ellis, G C Sharp, P F Agris","doi":"10.3109/08820138409025456","DOIUrl":"https://doi.org/10.3109/08820138409025456","url":null,"abstract":"A clinical laboratory carrying out tests for antinuclear antibodies requires an efficient, reliable preparation method to produce a high yield of nuclear antigens at low cost and a very sensitive, specific assay method for antigen activity. Various tissues were employed for preparation of small nuclear ribonucleoprotein (snRNP) and Sm antigens for these purposes. Fresh calf thymus cells and nuclei, commercially available calf and rabbit thymus acetone powders, fresh rat kidney and liver cells were used as sources of antigens prepared similarly by methods published previously. Preparations of antigens from whole calf thymus cell extracts were prepared with and without inhibitors to protease and RNase. snRNP and Sm antigens were assayed at each preparation step by hemagglutination inhibition (HAI). Using HAI it was possible to routinely assay snRNP and Sm at nanogram/ml quantities which was 10(6) fold more sensitive than Ouchterlony immunodiffusion. Results were expressed as relative specific activity as compared with calf thymus nuclear extract prepared by conventional methods. Protease and RNase inhibitors did not significantly increase yields. Thymus was the best source of snRNP and Sm. Fresh calf thymus extract produced a good, stable, reliable quantity of antigens, whereas calf and rabbit thymus acetone powders provided antigen at higher specific activity with less labor but slightly lower yields. Thus, considering the total cost of preparations, commercial sources may be superior to fresh sources in the clinical laboratory setting. These studies also revealed the utility of the sensitive HAI test not only in the clinical laboratory but also for further research endeavors.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"13 2","pages":"127-36"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138409025456","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17298369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Antigen-antibody-complement reaction studies on micro bilayer lipid membranes. 微双分子层脂质膜的抗原-抗体-补体反应研究。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409025453

K P O'Boyle, F A Siddiqi, H T Tien

引用次数: 16

Inhibition of rat mammary tumor growth by purified protein A--a potential anti-tumor agent. 纯化蛋白A-一种潜在的抗肿瘤剂对大鼠乳腺肿瘤生长的抑制作用。

Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051962

P K Ray, S K Bandyopadhyay

{"title":"Inhibition of rat mammary tumor growth by purified protein A--a potential anti-tumor agent.","authors":"P K Ray, S K Bandyopadhyay","doi":"10.3109/08820138309051962","DOIUrl":"https://doi.org/10.3109/08820138309051962","url":null,"abstract":"Intravenous inoculations of purified Protein A of Staphylococcus aureus Cowan I cause significant (p less than 0.01) regression of di-methyl-benz-anthracene (DMBA)-induced mammary adenocarcinomas in Sprague-Dawley rats. Direct tumor cell counts of treated tumors showed fewer (p less than 0.005) viable tumor cells than did tumors from untreated controls. Plasma immunoglobulin G concentration showed a significant (p less than 0.05) increase compared to that of controls. However, the concentration of immune complexes and percentages of T-rosettes did not change. Peripheral blood mononuclear cells (PBMNC) from treated animals showed increased cytotoxicity (p less than 0.005) compared to that of controls. Plasma of treated animals potentiated PBMNC cytotoxicity (p less than 0.05) and showed increased antibody and complement-mediated cytotoxicity (p less than 0.025). The exact mechanism of protein A-induced potentiation of anti-tumor immune reactivities leading to tumoricidal response is not known. However, our data are suggestive of the involvement of both cellular and humoral immunity of the host in the tumor regressive phenomenon.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"453-64"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051962","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17475307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Cleavage of protein A-binding IgA1 with IgA1 protease from Streptococcus sanguis. 血链球菌中蛋白a结合IgA1与IgA1蛋白酶的裂解。

Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051966

J Biewenga, F Daus

引用次数: 4

Glutaurine enhances the depressed NK cell activity of tumor patients. 谷胱甘肽增强肿瘤患者抑制NK细胞活性。

Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051969

I Láng, L Feuer, K Nékám, A Szigeti, P Gergely, G Petrányi

引用次数: 2