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Measurement of binding constants for divalent antibody-immobilized antigen interaction by antigen-sepharose 4B chromatography. 用抗原-sepharose 4B色谱法测定二价抗体-固定化抗原相互作用的结合常数。
Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051970
M R Bruera, D Sevlever, C A Gatti
{"title":"Measurement of binding constants for divalent antibody-immobilized antigen interaction by antigen-sepharose 4B chromatography.","authors":"M R Bruera,&nbsp;D Sevlever,&nbsp;C A Gatti","doi":"10.3109/08820138309051970","DOIUrl":"https://doi.org/10.3109/08820138309051970","url":null,"abstract":"<p><p>Binding constants for the human IgG-anti human IgG heavy chain were determined from elution profiles obtained by loading human IgG-Sepharose 4B columns with the antibody and eluting it at different NaI concentrations. The agreement between the value obtained at zero NaI concentration and the value determined in solution by equilibrium molecular sieving was good. An application of this column method should be useful for comparison of avidity values of antibody populations of the same specificity.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"529-38"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051970","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17475308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Human lymphocyte agglutinins in Tursiops truncatus (bottlenose dolphin). 宽吻海豚的人淋巴细胞凝集素。
Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051965
D Hohn, E Cohen, R Cunningham, J Fitzpatrick
{"title":"Human lymphocyte agglutinins in Tursiops truncatus (bottlenose dolphin).","authors":"D Hohn,&nbsp;E Cohen,&nbsp;R Cunningham,&nbsp;J Fitzpatrick","doi":"10.3109/08820138309051965","DOIUrl":"https://doi.org/10.3109/08820138309051965","url":null,"abstract":"<p><p>A naturally occurring heterophile agglutinin directed against human erythrocytes and lymphocytes is present in the serum of the marine mammal, Tursiops truncatus (Bottlenose dolphin). Differential specificity was demonstrated with the use of absorption techniques that showed at least 3 separate specificities directed against erythrocytes, T cells, and B cells, of which the B cell agglutinin was in the highest titer. Isolation techniques employing ion exchange and affinity chromatography have shown these agglutinins to be of the IgM class. Agglutinin activity is lost when lymphocytes are treated with pronase, suggesting that the surface receptor is protein or protein associated.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"481-90"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051965","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17661585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Bacteria-immune system interactions. II. Adherence of Acinetobacter calcoaceticus strains to human lymphocytes. 细菌与免疫系统的相互作用。2钙酸不动杆菌在人淋巴细胞上的粘附。
Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051964
R Kleinman, A Piha
{"title":"Bacteria-immune system interactions. II. Adherence of Acinetobacter calcoaceticus strains to human lymphocytes.","authors":"R Kleinman,&nbsp;A Piha","doi":"10.3109/08820138309051964","DOIUrl":"https://doi.org/10.3109/08820138309051964","url":null,"abstract":"<p><p>Out of twenty-nine strains of Acinetobacter calcoaceticus (A. calcoaceticus) only eight adhered to lymphocytes. The percentage of lymphocytes labeled with bacteria varied from 5 to 70%. Most of the labeled lymphocytes had large numbers of bacteria attached to them. The variation in the percentages of lymphocytes labeled in various individuals was minimal.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"473-80"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051964","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17697340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A rapid assay for immunoglobulin in hybridoma supernatants. 杂交瘤上清液中免疫球蛋白的快速测定。
Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051963
D Male, G Pryce
{"title":"A rapid assay for immunoglobulin in hybridoma supernatants.","authors":"D Male,&nbsp;G Pryce","doi":"10.3109/08820138309051963","DOIUrl":"https://doi.org/10.3109/08820138309051963","url":null,"abstract":"<p><p>A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"465-71"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051963","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17418729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Preparation of microspheres of poly(glycidyl methacrylate) and its derivatives as carriers for immobilized proteins. 聚甲基丙烯酸缩水甘油酯微球及其衍生物作为固定化蛋白质载体的制备。
Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051968
S Hosaka, Y Murao, S Masuko, K Miura
{"title":"Preparation of microspheres of poly(glycidyl methacrylate) and its derivatives as carriers for immobilized proteins.","authors":"S Hosaka,&nbsp;Y Murao,&nbsp;S Masuko,&nbsp;K Miura","doi":"10.3109/08820138309051968","DOIUrl":"https://doi.org/10.3109/08820138309051968","url":null,"abstract":"<p><p>Microspheres of poly(glycidyl methacrylate) and its derivatives have been prepared. The diameter can be varied in the range from 0.5 to 5 micron. Proteins were immobilized on the microspheres by various processes. The maximum amount of covalently immobilized gamma-globulin on the microspheres was found to be about 6 mg/m2. It was shown that dyed microspheres with a diameter of 2-4 micron are applicable to immunological agglutination tests on a microplate as carrier particles substituting for red blood cells.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"509-17"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051968","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17697202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
New Thy-1- and H-2-congenic strains of mice and their application in studies on the mechanism of anti-Thy-1.1 response. 新的小鼠Thy-1和h -2基因菌株及其在抗thy -1.1反应机制研究中的应用。
Immunological communications Pub Date : 1983-10-01 DOI: 10.3109/08820138309051967
J S Reichner, T J Gorzynski, M B Zaleski
{"title":"New Thy-1- and H-2-congenic strains of mice and their application in studies on the mechanism of anti-Thy-1.1 response.","authors":"J S Reichner,&nbsp;T J Gorzynski,&nbsp;M B Zaleski","doi":"10.3109/08820138309051967","DOIUrl":"https://doi.org/10.3109/08820138309051967","url":null,"abstract":"<p><p>The magnitude of the primary humoral anti-Thy-1.1 responses was measured by the number of PFC in spleens of B10.S(7R) mice and some of their H-2 heterozygous F1 hybrids immunized with thymocytes of either AKR.TH or B10.S(7R)-Thy-1a strain. These two of the five newly developed strains are non-H-2 incompatible and non-H-2 compatible with B10.S(7R) mice, respectively. The low anti-Thy-1.1 responses of B10.S(7R) mice and their F1 hybrids after immunization with thymocytes from non-H-2 compatible B10.S(7R)-Thy-1a donors indicated that the I-As \"allele\" of B10.S(7R) mice and the I-A \"alleles\" of other parental strains tested did not produce a complementary effect that is required for non-H-2 compatible thymocytes to elicit a good anti-Thy-1 response.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"501-8"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051967","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17206035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Adaptation of a protein a binding assay for measurement of immune complexes in sera of rats. 大鼠血清中免疫复合物测定的蛋白质适应性结合试验。
Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309066870
W M Siag, J M Jones
{"title":"Adaptation of a protein a binding assay for measurement of immune complexes in sera of rats.","authors":"W M Siag,&nbsp;J M Jones","doi":"10.3109/08820138309066870","DOIUrl":"https://doi.org/10.3109/08820138309066870","url":null,"abstract":"<p><p>We describe a simple and sensitive method for the detection of circulating immune complexes (CIC) in rats and have applied the method to test sera from tumor-bearing rats. CIC were precipitated preferentially with 6% polyethylene glycol, dissolved, and then bound to Staphylococcus aureus that are Protein A (PA) positive. Rabbit-anti-rat IgG (RARG) antibodies were then added and followed by 125I-PA. The RARG sandwich antibody enhanced the sensitivity of detection of CIC in rats at least 5 fold compared to that observed using 125I-PA without RARG.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 2","pages":"215-21"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309066870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17660010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetically-controlled severity of autoimmune thyroiditis in Obese strains (OS) chickens is expressed at both the humoral and cellular effector mechanism levels. 肥胖株(OS)鸡自身免疫性甲状腺炎的遗传控制严重程度在体液和细胞效应机制水平上表达。
Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050748
R L Boyd, R K Cole, G Wick
{"title":"Genetically-controlled severity of autoimmune thyroiditis in Obese strains (OS) chickens is expressed at both the humoral and cellular effector mechanism levels.","authors":"R L Boyd,&nbsp;R K Cole,&nbsp;G Wick","doi":"10.3109/08820138309050748","DOIUrl":"https://doi.org/10.3109/08820138309050748","url":null,"abstract":"<p><p>Two lines of Obese strain (OS) chickens of identical MHC (B) genotype, B5B5, bred over 10 years with different selection parameters, differ in their severity of spontaneous autoimmune thyroiditis. To determine whether alterations in immune responsiveness underly this discrepancy, the two lines were compared for their thyroiditis effector mechanisms. The OS B5B5 chickens, selected for high levels of serum thyroglobulin autoantibody, had correspondingly higher levels of thyroid-specific cytotoxic cells and also antibody dependent cellular cytotoxicity (ADCC) than the equivalent B5B5 line selected solely for the phenotypic trait of hypothyroidism. These results thus emphasize the importance of the non-MHC locus controlling immune responsiveness, in the 3 locus-model for this autoimmune disorder.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 3","pages":"263-72"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309050748","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17934671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Level of macrophage induction during tumor growth: primed or activated? 巨噬细胞在肿瘤生长过程中的诱导水平:启动还是激活?
Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309050750
C J Burger, K D Elgert
{"title":"Level of macrophage induction during tumor growth: primed or activated?","authors":"C J Burger,&nbsp;K D Elgert","doi":"10.3109/08820138309050750","DOIUrl":"https://doi.org/10.3109/08820138309050750","url":null,"abstract":"<p><p>The activation level of thioglycolate (TG)-elicited peritoneal macrophages (Mphi) from normal and fibrosarcoma-bearing BALB/c mice was investigated. Lipopolysaccharide was used to distinguish in vitro levels of Mphi activation. The results of cytotoxicity assays, which measured nonspecific killing of P815 tumor cells by activated Mphi, indicated that tumor-bearing host Mphi were primed in situ while normal host Mphi remained in a resting state. This level of Mphi induction may contribute to the resulting tumor-bearing host T cell immune hyporesponsiveness.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 3","pages":"285-90"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309050750","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17934672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Production of monoclonal antibodies to surface regions that are non-immunogenic in a protein using free synthetic peptide as immunogens: demonstration with sperm-whale myoglobin. 利用自由合成肽作为免疫原生产针对蛋白质表面非免疫原性区域的单克隆抗体:用抹香鲸肌红蛋白进行演示。
Immunological communications Pub Date : 1983-01-01 DOI: 10.3109/08820138309066866
H E Schmitz, H Atassi, M Z Atassi
{"title":"Production of monoclonal antibodies to surface regions that are non-immunogenic in a protein using free synthetic peptide as immunogens: demonstration with sperm-whale myoglobin.","authors":"H E Schmitz,&nbsp;H Atassi,&nbsp;M Z Atassi","doi":"10.3109/08820138309066866","DOIUrl":"https://doi.org/10.3109/08820138309066866","url":null,"abstract":"<p><p>Two synthetic peptides corresponding to surface regions of sperm whale myoglobin that are not antigenic in the native molecule were used in their free form (i.e. not coupled to a carrier) to immunize separate groups of Balb/cByJ mice. The synthetic peptides corresponded to regions 1-6 and 121-127. Serum samples obtained from each group of mice contained antibodies that bound specifically to myoglobin and exclusively to the immunizing peptide. Monoclonal antibodies to each of the two surface regions were subsequently obtained by hybridizing Fa/O mouse myeloma cells with spleen cells derived from each group of mice. These monoclonal antibodies were IgM (kappa). They expressed the same isotype as the antigen specific serum antibodies produced by the mice whose spleen cells were used for hybridization. Solid phase radioimmunoassay studies also indicated that each monoclonal antibody, like the immune serum of the parent animals, bound specifically to myoglobin and exclusively to the synthetic peptide used as an immunogen. These results suggested that the hybridoma antibodies expressed submolecular binding specificities that were the result of peptide immunization rather than hybrid selection and that monoclonal antibodies with preselected submolecular binding specificities to non-antigenic surface regions in a protein molecule can be produced by the techniques of somatic cell hybridization when the corresponding free synthetic peptides are used as immunogens.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 2","pages":"161-75"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309066866","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17256323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 35
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