杂交瘤上清液中免疫球蛋白的快速测定。

Immunological communications Pub Date : 1983-10-01 DOI:10.3109/08820138309051963

D Male, G Pryce

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引用次数: 1

摘要

描述了一种单步测定法，可以在浓度低于100 ng/ml的组织培养上清中检测IgM和IgG。该检测是基于葡萄球菌蛋白A包被羊红细胞(E-spA)在增强抗体存在下的凝集。该试验设计用于快速筛选杂交瘤培养产生的抗体。

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A rapid assay for immunoglobulin in hybridoma supernatants.

A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.

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Immunological communications

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