{"title":"A rapid assay for immunoglobulin in hybridoma supernatants.","authors":"D Male, G Pryce","doi":"10.3109/08820138309051963","DOIUrl":null,"url":null,"abstract":"<p><p>A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"465-71"},"PeriodicalIF":0.0000,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051963","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08820138309051963","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.
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