T cell recognition of lysozyme. II. Shift in specificity during long-term culture determined by synthetic overlapping peptides comprising the entire protein chain.
{"title":"T cell recognition of lysozyme. II. Shift in specificity during long-term culture determined by synthetic overlapping peptides comprising the entire protein chain.","authors":"G S Bixler, T Yoshida, M Z Atassi","doi":"10.3109/08820138409025459","DOIUrl":null,"url":null,"abstract":"<p><p>Recently, by using a comprehensive synthetic strategy developed in this laboratory, we localized four sites (T sites) within the polypeptide chain of lysozyme recognized by T cells from two high responder mouse strains, DBA/1 and B10.BR. However, to detect minor specificities, the selective enrichment of lysozyme reactive cells, would be required. T cells from long-term cultures maintained by repeated stimulation with antigen are selectively enriched for that antigen. It is not known whether maintaining T cells for extended periods of time in vitro has any consequences on the profile of T cell recognition. In the present study, T cells from long-term cultures, derived from these two high responder lysozyme-primed mouse strains, were examined for their responsiveness to a series of synthetic overlapping peptides encompassing the entire polypeptide chain of the lysozyme molecule. We have found that the profile of T cell recognition of the long-term cultures may not reflect that of the lysozyme primed lymph node cells, but that it is subject to a shift in specificity towards submolecular features of the molecule. In addition, we have identified in B10.BR mice another region within the polypeptide chain of lysozyme (residues 72-84) which may potentially harbor a previously undetected (in lymph node cells) minor T site.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"13 2","pages":"161-72"},"PeriodicalIF":0.0000,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138409025459","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08820138409025459","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
Abstract
Recently, by using a comprehensive synthetic strategy developed in this laboratory, we localized four sites (T sites) within the polypeptide chain of lysozyme recognized by T cells from two high responder mouse strains, DBA/1 and B10.BR. However, to detect minor specificities, the selective enrichment of lysozyme reactive cells, would be required. T cells from long-term cultures maintained by repeated stimulation with antigen are selectively enriched for that antigen. It is not known whether maintaining T cells for extended periods of time in vitro has any consequences on the profile of T cell recognition. In the present study, T cells from long-term cultures, derived from these two high responder lysozyme-primed mouse strains, were examined for their responsiveness to a series of synthetic overlapping peptides encompassing the entire polypeptide chain of the lysozyme molecule. We have found that the profile of T cell recognition of the long-term cultures may not reflect that of the lysozyme primed lymph node cells, but that it is subject to a shift in specificity towards submolecular features of the molecule. In addition, we have identified in B10.BR mice another region within the polypeptide chain of lysozyme (residues 72-84) which may potentially harbor a previously undetected (in lymph node cells) minor T site.