Immunological communications最新文献_第4页

Polymeric microspheres for immunoresearch. 免疫研究用高分子微球。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409025455

M Kumakura, I Kaetsu

引用次数: 4

Interleukin 3 activity in tumor-bearing hosts: decreased splenocyte production of and responsiveness to IL 3. 白细胞介素3在荷瘤宿主中的活性:降低脾细胞产生和对白细胞介素3的反应。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409025466

C J Burger, K D Elgert

{"title":"Interleukin 3 activity in tumor-bearing hosts: decreased splenocyte production of and responsiveness to IL 3.","authors":"C J Burger, K D Elgert","doi":"10.3109/08820138409025466","DOIUrl":"https://doi.org/10.3109/08820138409025466","url":null,"abstract":"A kinetic study assessing the relationship between tumor growth and the ability of BALB/c mouse splenocytes to produce Interleukin 3 (IL 3) indicated a concomitant decrease in IL 3 activity with tumor growth. Tumor-bearing host (TBH) splenocytes produced 600 pmoles/hr/10(8) cells of IL 3 activity at Day 0 but only 62 pmoles/hr/10(8) cells by Day 28 post tumor cell inoculation. Nylon wool fractionation (to remove adherent suppressor cells) did not restore IL 3 activity. Addition of purified IL 3 to mitogen proliferation assays showed that IL 3 alone was mitogenic for normal host but not TBH splenocytes. In concert with concanavalin A (Con A) and phytohemagglutinin, IL 3 augmented in vitro normal host splenocyte responsiveness but significantly further suppressed it in the TBH. An absorption assay indicated that fresh cells had acceptors to remove IL 3 from supernatants. Con A-induced normal or TBH blast cells lost their ability to absorb IL 3. Intravenous inoculation of purified IL 3 into normal and TBH resulted in further suppression of TBH splenocyte mitogen-induced blastogenesis. The exacerbation of TBH spleen cell reactivity by IL 3 may be due to a tumor-induced feedback inhibition mechanism further suppressing cellular differentiation critical to cytotoxic T lymphocyte maturation.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"13 3","pages":"255-68"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138409025466","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17489685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Distribution of P2 antigen in neural and non-neural bovine tissues. P2抗原在牛神经和非神经组织中的分布。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409048668

R B Eaton, W Campbell, F Milgrom

引用次数: 1

Preservation of Fc gamma receptor-mediated particle phagocytosis and binding with rat alveolar macrophages adhered to a plastic substrate in a serum free system. 在无血清系统中，Fc γ受体介导的颗粒吞噬作用的保存及其与粘附于塑料底物的大鼠肺泡巨噬细胞的结合。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409048666

B E Lehnert, P E Morrow

引用次数: 9

Antigen-antibody reactions in the eye involving complement binding IgG antibodies and their non-complement binding F(ab')2 fragments. 眼睛中的抗原抗体反应涉及补体结合IgG抗体及其非补体结合F(ab')2片段。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409048667

S Aron, A Hofeldt, W Manski

引用次数: 1

Tetanus toxoid reactive T lymphocytes in the cerebrospinal fluid of multiple sclerosis patients. 多发性硬化症患者脑脊液破伤风类毒素反应性T淋巴细胞的研究。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409048670

J Burns, B Zweiman, R Lisak

引用次数: 10

Protein immunochemistry for the sophisticate. 高级蛋白质免疫化学。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409061310

R P Pelley

引用次数: 0

Dissociation of stimulatory capacity in mixed lymphocyte reaction and proliferative responses to pokeweed mitogen among peripheral blood non-T cells. 外周血非t细胞对美洲商陆丝裂原的混合淋巴细胞反应和增殖反应中刺激能力的分离。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409061307

H S Ko, D Ohene-Fianko

引用次数: 0

New avenues in the use of cyclosporine for transplantation. Graft and donor pretreatment. 环孢素用于移植的新途径。移植物和供体预处理。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409061304

J Cederna, L H Toledo-Pereyra, S Gof, A Rios, G H MacKenzie, D A Gordon

{"title":"New avenues in the use of cyclosporine for transplantation. Graft and donor pretreatment.","authors":"J Cederna, L H Toledo-Pereyra, S Gof, A Rios, G H MacKenzie, D A Gordon","doi":"10.3109/08820138409061304","DOIUrl":"https://doi.org/10.3109/08820138409061304","url":null,"abstract":"Modification of graft immunogenicity using graft (GPTX) and donor pretreatment (DPTX) has been pursued in an attempt to modify allograft immunogenicity using various immunosuppressive agents. The murine skin allograft and canine renal allograft models were used to study the efficacy of Cyclosporine (Cy A) as a DPTX and GPTX prior to transplantation. Tail skin allografts from C3HHeN male mice were grafted to Balb/c female mouse recipients. Minimal immunosuppression was given to all skin graft recipients. Skin allograft were either GPTX with Cy A, DPTX with either Cy A, methylprednisolone (MP), or cyclophosphamide (CP), or Cy A GPTX and DPTX with the three drugs alone or in combination. Cy A GPTX alone of skin allografts did not significantly prolong survival. DPTX with Cy A significantly prolonged skin graft survival, however, CP or MP alone did not. The various combinations of MP, Cy A, and CP as DPTX and MP, Cy A, and CP DPTX used together with Cy A GPTX also significantly prolonged murine skin allograft survival. Kidney allografts used unrelated mongrel dogs as donors or recipients. Renal transplant experimental groups were either: Non-pretreated and immediately transplanted, nonpretreated and hypothermically stored (HS) for 24 hours in Collins (C-2) solution, GPTX with 12.5 mg Cy A during 24 hr. HS in C-2, DPTX with Cy A (25 mg/Kg), or Cy A DPTX (15 mg/kg) and GPTX during 24 hrs. HS in C-2. Cy A GPTX during HS was sometimes effective in prolonging kidney allograft survival greater than 30 days using only minimal immunosuppression with azathioprine. Cy A DPTX prolonged survival somewhat, but not significantly. Improved results were seen, however, when Cy A DPTX was used together with Cy A graft pretreatment. These results indicate the potential for the successful use of Cy A as a donor and/or graft pretreatment, however, further studies will be necessary to optimize the use of Cy A in these modalities.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"13 6","pages":"523-38"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138409061304","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17458375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Coexpression of multiple immunoglobulin isotypes on human B-lymphocytes. 多种免疫球蛋白同型在人b淋巴细胞上的共表达。

Immunological communications Pub Date : 1984-01-01 DOI: 10.3109/08820138409033887

C C Hsu

{"title":"Coexpression of multiple immunoglobulin isotypes on human B-lymphocytes.","authors":"C C Hsu","doi":"10.3109/08820138409033887","DOIUrl":"https://doi.org/10.3109/08820138409033887","url":null,"abstract":"Endogenous immunoglobulin (Ig) determinants on blood B-lymphocytes (B-cells) were investigated in 13 healthy individuals, 9 patients with thyrotoxic Graves disease, 5 patients with chronic sarcoidosis, and 4 patients with IgA deposition in renal glomeruli. Specificities of goat antisera to Ig determinants were confirmed by studying Ig isotypes on leukemic B-cells. Absence of nonspecific attachment of the goat antisera was ascertained by reacting cells with goat IgG. Lymphocytes were distinguished from monocytes by morphology and by reacting monocytes with rhodamine-conjugated immune complexes. The endogenous nature of the cell surface Ig was established by an antibody-prelabeling technique as follows: after the surface Ig had been labelled with fluorescent antibody, the cells were cultured for 3 days. Antibody-prelabelled surface Ig diminished by the third day of incubation because of shedding. Thus restaining of the cells at the end of the culture identified the membrane Ig determinants expressed during the incubation. Our results indicated that endogenous gamma and alpha chains were present on B-cells of all donors. In Graves disease, epsilon chain was also found. In all cases of Graves disease, 2 cases of sarcoidosis and 2 normal individuals, gamma, alpha, mu and delta chains were present on the majority of B-cells suggesting coexpression of these heavy chains on a single cell. I conclude that all 5 Ig isotypes may be coexpressed on B-cells under certain clinical conditions.","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"13 5","pages":"403-18"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138409033887","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17500638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5