IET nanobiotechnology最新文献

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148087458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic Comparison of Droplet-Based and Microwell-Based Platforms for Comprehensive Single-Cell Transcriptomic Analysis in Clinical Samples","authors":"Shuai Wang,&nbsp;Yuxian Feng,&nbsp;Qiongdan Zhang,&nbsp;Yue Cui,&nbsp;Yi Qiao,&nbsp;Hao Huang,&nbsp;Xuan Pan,&nbsp;Jing Tu","doi":"10.1049/nbt2/9314222","DOIUrl":"10.1049/nbt2/9314222","url":null,"abstract":"<p>Single-cell RNA sequencing (scRNA-seq) is widely utilized in tumor research. However, platform-specific technical biases may impact data interpretation. This study compared the performance of droplet- and microwell-based scRNA-seq platforms in the analysis of clinical samples. Despite the similarities after batch effect correction, significant differences were observed in multiple aspects, including mRNA preference, cell type restoration, and gene expression patterns. The droplet-based platform captured a higher proportion of immune cells, whereas the microwell-based platform provided a more accurate immune cell representation. Differential gene expression, pseudotime, and cell–cell communication analyses further revealed platform-dependent variations across multiple aspects. Overall, this study provides valuable insights into platform selection and optimization for cross-platform data integration in single-cell transcriptomics.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12933413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147305395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148094300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148081349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic Insights Into Protein Aggregation Inhibition by Green-Synthesized Silver Nanoparticles: A Study on Human Lysozyme","authors":"Md. Tauqir Alam,&nbsp;Mohd. Ahmar Rauf,&nbsp;Arman Khan,&nbsp;Rizwan Hussain","doi":"10.1049/nbt2/2694374","DOIUrl":"10.1049/nbt2/2694374","url":null,"abstract":"<p>A characteristic of many neurodegenerative disorders, such as Parkinson’s and Alzheimer’s, is amyloidogenic protein aggregation, for which there are currently no proven cures. Aging, mutation, and physiological stress can cause proteins to deviate from their natural folding patterns, potentially leading to the formation of hazardous protein aggregates. Noble metal nanoparticles (NPs), due to their unique physicochemical properties, have emerged as promising tools in biomedicine, with applications ranging from tissue engineering to drug delivery and diagnostics. Although concerns regarding cytotoxicity exist, small-sized silver (Ag) NPs (AgNPs) have demonstrated potential in antiviral, anticancer, and antibacterial therapies. This study investigated the development of biocompatible AgNPs using a green synthesis approach and examined their chaperone-like activity against protein aggregation, emphasizing the role of meticulous in vitro design. Human lysozyme (HLZ) served as a model protein for aggregation inhibition assays. Biogenic AgNPs exhibited a concentration-dependent effect on HLZ aggregation, demonstrating an optimal inhibitory concentration, followed by a decrease in efficacy at higher concentrations. Furthermore, astrocytes treated with AgNPs displayed reduced protein aggregation, suggesting a chaperone-like behavior. The initial phase focused on the detailed characterization of AgNPs synthesized using orange juice extract. Subsequently, this study explored the mechanistic understanding of AgNP-mediated inhibition of protein aggregation under controlled conditions. A battery of biophysical techniques, including circular dichroism (CD), 8-anilino-1-naphthalene-sulfonic acid (ANS) fluorescence, thioflavin T (ThT) fluorescence, Congo red (CR) assay, and turbidity measurements, was employed to meticulously assess the inhibitory effect on HLZ aggregation in vitro.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12809263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148068319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Antiviral Potential of Tungsten Oxide Nanoparticles Against Herpes Simplex Virus Type 1: A Promising Alternative to Acyclovir","authors":"Abdulhussain Kadhim Jwaziri,&nbsp;Pegah Khales,&nbsp;Seyed Jalal Kiani,&nbsp;Homayoun Yaghouti,&nbsp;Roghayeh Babaei,&nbsp;Zahra Salavatiha,&nbsp;Ahmad Tavakoli","doi":"10.1049/nbt2/6697780","DOIUrl":"10.1049/nbt2/6697780","url":null,"abstract":"<p>Herpes simplex virus type 1 (HSV-1) is responsible for the majority of cold sores, herpetic keratitis-induced blindness, profound skin lesions, and encephalitis that can be fatal. Currently, acyclovir and its derivatives are the first-line therapy for the treatment of HSV-1 infection. But there are drawbacks to these treatments: limited efficacy against drug-resistant strains of the virus. Hence, it is of critical importance to explore and develop new antiviral drugs for HSV-1. In the present study, we explored whether tungsten oxide nanoparticles (WO₃NPs) were potent inhibitors of HSV-1 infection as a new class of agent. WO₃NPs were characterized by X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FTIR) spectroscopy, and zeta potential analysis. Cytotoxicity of Vero cells caused by WO₃NPs was determined by methyl thiazolyl tetrazolium (MTT) assay. The quantitative real-time polymerase chain reaction (qRT-PCR) assay was utilized for further verification of the action of the WO₃NPs on HSV-1. The cytotoxicity test showed low toxicity (&lt;20%) of the rod-shaped WO₃NPs when they were assayed on Vero cells at concentrations of up to 700 μg/mL. When HSV-1 was treated with WO₃NPs at 700 µg/mL [20% cytotoxicity concentration (CC₂₀); the concentration causing 20% cytotoxicity, ~80% cell viability] and 1000 µg/mL [50% cytotoxicity concentration (CC₅₀); the concentration causing 50% cytotoxicity, ~50% cell viability] for 3 h, the viral load was significantly reduced, achieving inhibition rates of 99.4% and 99.9%, respectively. Additionally, experiments conducted after HSV-1 infection of Vero cells (post-treatment assays) indicated that WO₃NPs at concentrations of 250, 500, and 750 µg/mL significantly suppressed viral replication, with inhibition rates of 82%, 87.5%, and 96.5%, respectively. WO₃NPs have potent inhibitory effects on HSV-1. Therefore, they can be considered potential candidates for therapeutic development against infections caused by this virus.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148061569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication, Characterization, and Antifungal Activity of Chitosan–Cyproconazole Nanocomposite for Simultaneous Wheat Stem Rust Control and Growth Enhancement","authors":"Jafar Fathi-Qarachal,&nbsp;Seyed Ali Moosawi-Jorf,&nbsp;Maryam Nikkhah,&nbsp;Mansoor Karimi-Jashni","doi":"10.1049/nbt2/6628425","DOIUrl":"10.1049/nbt2/6628425","url":null,"abstract":"<p>The stem rust disease caused by obligate biotrophic fungus <i>Puccinia graminis</i> f. sp. <i>tritici</i> is a worldwide threat to the global wheat production with frequent epidemics leading to widespread reliance on chemical fungicides such as cyproconazole. To reduce fungicide risks on human health and environmental integrity, chitosan nanoparticles (CNPs) and novel chitosan–cyproconazole nanocomposite (Chi-Cyp) were synthesized. Dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy confirmed the size of 80–90 nm and surface charge and uniformity. To evaluate their efficacy against the disease, various concentrations of CNP and Chi-Cyp were applied via irrigation, foliar spray, and a combination of both methods. Wheat seedlings were treated 24 h prior to inoculation, as well as at 48- and 96-h post-inoculation with Pgt urediniospores. Phenotypic assessments conducted 2 weeks post-inoculation revealed that CNPs (100 μg/mL) and Chi-Cyp (1 μg/mL), along with the positive control cyproconazole (10 μg/mL), significantly suppressed stem rust infection. Quantitative polymerase chain reaction (qPCR) analysis corroborated these findings, demonstrating a substantial reduction in fungal biomass in treated plants. Additionally, the impact of the nanomaterials on plant growth parameters was examined. Notably, Chi-Cyp treatment at 50 μg/mL significantly enhanced seedling growth, as evidenced by increased shoot and root lengths, and elevated fresh and dry biomass accumulation. This study highlights the potential of the Chi-Cyp nanocomposite, which contains a 10-fold lower concentration of cyproconazole, to effectively control stem rust with comparable efficacy to the fungicide alone. These findings underscore the promise of nanotechnology-based strategies in sustainable plant disease management.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2/6628425","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Dioscin-loaded zein nanoparticles alleviate lipopolysaccharide-induced acute kidney injury via the microRNA-let 7i signalling pathways.","authors":"Iet Nanobiotechnology","doi":"10.1049/nbt2/9767341","DOIUrl":"10.1049/nbt2/9767341","url":null,"abstract":"","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"2026 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13347625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}