IET nanobiotechnology最新文献

{"title":"Poly lactic-co-glycolic acid-alginate nanocarrier for efficient drug delivery to liver cancer cells","authors":"Mahsa Hoseinzadeh,&nbsp;Mohammad Javad Mokhtari,&nbsp;Farshid Kafilzadeh,&nbsp;Javad Mohammadnejad,&nbsp;Yaghoob Taheri","doi":"10.1049/nbt2.12143","DOIUrl":"10.1049/nbt2.12143","url":null,"abstract":"<p>Efficient drug delivery systems (DDSs) can potentially replace with conventional modalities in cancer therapy, like liver cancer. In this study, a novel folic acid (FA)-functionalised and alginate (Alg)-modified poly lactic-co-glycolic acid (PLGA) nanocomposite was developed for delivery of doxorubicin (Dox) to HepG2 and Huh7 liver cancer cells. After synthesising the nanocarrier, several analytical devices, including FT-IR, DLS, TGA, and TEM, were employed for its characterisation. Nano-metric size (55 and 85 nm in diameter), close to neutral surface charge, semi-spherical morphology, and successful synthesis were approved. Dox entrapment efficiency was determined near 1%, and sustained and pH-sensitive drug release behaviours of nanocarrier were ascertained for DDS. Afterwards, the cell viability test was carried out to study the HepG2 and Huh7 cells suppression capability of FA-PLGA-Dox-Alg. About 12% and 10% cell viabilities were observed in HepG2 and Huh7 cancer cells after 24 h treatment with 400 nM concentration of FA-PLGA-Dox-Alg nanocarrier respectively. The IC₅₀ value was observed for 100 nM after 24 h of treatment in cancer cells. These data have indicated that fabricated nanocarrier could be promising DDS against liver cancer and replace with conventional approaches in cancer treatment, like chemotherapy.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 5","pages":"450-464"},"PeriodicalIF":2.3,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3e/f9/NBT2-17-450.PMC10374548.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9891813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and characterisation of a novel poly(2-hydroxyethylmethacrylate)-chitosan hydrogels loaded cerium oxide nanocomposites dressing on cutaneous wound healing on nursing care of chronic wound","authors":"Jingna Luo,&nbsp;Weijun Liu,&nbsp;Qiaoling Xie,&nbsp;Jianshu He,&nbsp;Liyan Jiang","doi":"10.1049/nbt2.12118","DOIUrl":"10.1049/nbt2.12118","url":null,"abstract":"<p>This study was designed to establish the composition of wound dressing based on poly(2-hydroxyethylmethacrylate)-chitosan (PHEM-CS) hydrogels-loaded cerium oxide nanoparticle (CeONPs) composites for cutaneous wound healing on nursing care of the chronic wound. The as-synthesised PHEM-CS/CeONPs hydrogels nanocomposites were characterised by using UV–visible spectroscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and thermo gravimetric analysis. The influence of PHEM-CS/CeONPs hydrogels nanocomposites on the gelation time, swelling ratio, in vitro degradation, and mechanical properties was investigated. The as-prepared PHEM-CS/CeONPs hydrogels nanocomposites dressing shows high antimicrobial activity against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. Similar trends were observed for the treatment of biofilms where PHEM-CS/CeONPs hydrogels nanocomposites displayed better efficiency. Furthermore, the biological properties of PHEM-CS/CeONPs hydrogels nanocomposites had non-toxic in cell viability and excellent cell adhesion behaviour. After 2 weeks, the wounds treated with the PHEM-CS/CeONPs hydrogels nanocomposite wound dressing achieved a significant closure to 98.5 ± 4.95% compared with the PHEM-CS hydrogels with nearly 71 ± 3.55% of wound closure. Hence, this study strongly supports the possibility of using this novel PHEM-CS/CeONPs hydrogels nanocomposites wound dressing for efficient cutaneous wound healing on chronic wound infection and nursing care.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 4","pages":"312-325"},"PeriodicalIF":2.3,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2.12118","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9706212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of solvent evaporation method in liposomal nanocarriers loaded-garlic essential oil (Allium sativum): Based on the encapsulation efficiency, antioxidant capacity, and instability","authors":"Salar Ali Ahmed,&nbsp;Mahmood Fadhil Saleem,&nbsp;Hamed Hassanzadeh","doi":"10.1049/nbt2.12142","DOIUrl":"10.1049/nbt2.12142","url":null,"abstract":"<p>This study is aimed to optimise the preparation factors, such as sonication time (5–20 min), cholesterol to lecetin ratio (CHLR) (0.2–0.8), and essential oil content (0.1–0.3 g/100 g) in solvent evaporation method for formulation of liposomal nanocarriers containing garlic essential oil (GEO) in order to find the highest encapsulation efficiency and stability with strongest antioxidant and antimicrobial activity. The droplet size, zeta potential, encapsulation efficiency, turbidity, changes in turbidity after storage (as a measure of instability), antioxidant capacity, and antimicrobial activity were measured for all prepared samples of nanoliposome. The sonication time is recognised as the most effective factor on the droplet size, zeta potential, encapsulation efficiency, turbidity, and instability while CHLR was the most effective factor on zeta potential and instability. The content of GEO significantly affected the antioxidant and antimicrobial activity in particular against gram-negative bacteria (<i>Escherichia coli</i>). The results of FTIR based on the identification of functional groups confirmed the presence of GEO in the spectra of the prepared nanoliposome and also it was not observed the interaction between the components of the nanoliposome. The overall optimum conditions were determined by response surface methodology (RSM) as the predicted values of the studied factors (sonication time: 18.99 min, CHLR: 0.59 and content of GEO: 0.3 g/100 g) based on obtaining the highest stability and efficiency as well as strongest antioxidant and antimicrobial activity.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 5","pages":"438-449"},"PeriodicalIF":2.3,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4e/44/NBT2-17-438.PMC10374552.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10268439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics and its applications in assisted reproductive technology","authors":"Jingying Gao,&nbsp;Yan Xiao","doi":"10.1049/nbt2.12141","DOIUrl":"10.1049/nbt2.12141","url":null,"abstract":"<p>Metabolomics, an emerging omics technology developed in the post-gene age, is an important part of systems biology. It interprets the pathophysiological state of the subject by quantitatively describing the dynamic changes of metabolites through analytical methods, mainly mass spectrometry (MS) and nuclear magnetic resonance (NMR). Assisted reproductive technology (ART) is a method used to manipulate sperm, oocytes, and embryos to achieve conception. Recently, several studies have reported that metabolomics methods can be used to measure metabolites in ART samples; these metabolites can be used to evaluate the quality of gametes and embryos. This article reviews the progress of research on metabolomics and the application of this technology in the field of ART, thus providing a reference for research and development directions in the future.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 5","pages":"399-405"},"PeriodicalIF":2.3,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c4/24/NBT2-17-399.PMC10374554.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10267952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guest Editorial: Selected extended papers from the 12th international conference on post-genomic technologies","authors":"Jing Tu,&nbsp;Lingzhi Wu,&nbsp;Qinyu Ge","doi":"10.1049/nbt2.12140","DOIUrl":"10.1049/nbt2.12140","url":null,"abstract":"&lt;p&gt;With the completion of the Human Genome Project in the 21st century, we have officially entered the era of post-genome technology. The rapid development of genomic technology is one of the fastest-growing and most influential cutting-edge technologies in the field of biomedical science. From high-throughput to single-molecule, from single-cell to multi-omics, from precision medicine to systems medicine, technologies in the post-genome era have triggered major changes in life science and medical research models, as well as medical clinical practice and related industries, providing a strong guarantee for human life and health.&lt;/p&gt;&lt;p&gt;Since the beginning of this century, in order to enhance academic exchanges in the field of advanced genomic technologies in the international academic community and cultivate innovative young talents, the series of International Conference on Post-Genomic Technologies have been organised. The 12&lt;sup&gt;th&lt;/sup&gt; International Conference on Post-Genomic Technologies was held online in October 2022. The current Special Issue is a collection of selected extended Papers from the 12th International Conference on Post-Genomic Technologies, which covers researches in the field of genome technology and related disciplines.&lt;/p&gt;&lt;p&gt;In this Special Issue, we have received six papers, all of which underwent peer review. Of these originally submitted papers, five have been accepted thus the overall submissions were of high quality, which marks the success of this Special Issue.&lt;/p&gt;&lt;p&gt;Han et al. present a strategy to prepare anionic liposomes without organic solvents for effective siRNA delivery. Organic solvents are necessary for the preparation of anionic liposomes for siRNA delivery. The remove of organic solvent is time-consuming and the residual organic solvent is not only a hidden danger, but also affects the stability of anionic liposomes. In this work, glycerol is successfully used to promote the dispersion of lipids and the formation of anionic liposomes with a spherical particle size of 188.9 nm. And with the help of Ca&lt;sup&gt;2+&lt;/sup&gt;, siRNA has been encapsulated in anionic liposomes. Therefore, anionic liposomes prepared with glycerol will be a safe and effective delivery platform for siRNA and even other nucleic acid drugs.&lt;/p&gt;&lt;p&gt;Lu et al. summarise the scRNA-seq data analysis method to improve analysis performance of noisy sequencing data. Some steps of the single-cell transcriptome analysis process have been highlighted, starting with the currently available single-cell transcriptome sequencing technologies, and the single-cell transcriptome sequencing data processing process, which describes the evaluation methods for single-cell transcriptome sequencing data processing methods. Overall, the manuscript provides some assistance to users when selecting methods and tools to process single-cell transcriptome data to take full advantage of scRNA-seq.&lt;/p&gt;&lt;p&gt;Gao et al. review the research progress of metabolomics and its applic","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 5","pages":"397-398"},"PeriodicalIF":2.3,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/51/NBT2-17-397.PMC10374553.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10221332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of pulmonary artery smooth muscle cells via the delivery of curcuminoid WZ35 by Cu-based metal organic frameworks","authors":"Zhidan Hua,&nbsp;Mingming Han,&nbsp;Lanlan Song,&nbsp;Yongle Yan,&nbsp;Honglang Chen,&nbsp;Jilong Wang,&nbsp;Chao Li,&nbsp;Yanfan Chen,&nbsp;Hanhan Yan,&nbsp;Mayun Chen","doi":"10.1049/nbt2.12138","DOIUrl":"10.1049/nbt2.12138","url":null,"abstract":"<p>Hypoxic pulmonary hypertension (HPH) is a life-threatening disease that occurs due to a lack of oxygen in the lungs, leading to an increase in pulmonary vascular resistance, right ventricular failure, and ultimately death. HPH is a multifactorial disorder that involves multiple molecular pathways, making it a challenge for clinicians to identify effective therapies. Pulmonary artery smooth muscle cells (PASMCs) play a crucial role in HPH pathogenesis by proliferating, resisting apoptosis, and promoting vascular remodelling. Curcumin, a natural polyphenolic compound, has shown potential as a therapeutic agent for HPH by reducing pulmonary vascular resistance, inhibiting vascular remodelling, and promoting apoptosis of PASMCs. Regulation of PASMCs could significantly inhibits HPH. However, curcumin has the disadvantages of poor solubility and low bioavailability, and its derivative WZ35 has better biosafety. Here, Cu-based metal organic frameworks (MOF_Cu) was fabricated to encapsulate the curcumin analogue WZ35 (MOF_Cu@WZ35) for the inhibition of PASMCs proliferation. The authors found that the MOF_Cu@WZ35 could promote the death of PASMCs. Furthermore, the authors believed that this drug delivery system will effectively alleviate the HPH.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 5","pages":"420-424"},"PeriodicalIF":2.3,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2.12138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9892051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lawsone encapsulated polylactic-co-glycolic acid nanoparticles modified with chitosan-folic acid successfully inhibited cell growth and triggered apoptosis in Panc-1 cancer cells","authors":"Helia Ghafaripour,&nbsp;Masoud Homayouni Tabrizi,&nbsp;Ehsan Karimi,&nbsp;Niloofar Barati Naeeni","doi":"10.1049/nbt2.12139","DOIUrl":"10.1049/nbt2.12139","url":null,"abstract":"<p>The present research aims to encapsulate lawsone in polylactic-co-glycolic acid (PLGA) nanoparticles modified with folic acid (FA) and chitosan (CS) to study its anticancer effects against Panc-1 cells. The nanoparticles were analysed in means of shape/size and zeta potential index using scanning electron microscope and dynamic light scattering. High-performance liquid chromatography was applied to evaluate the lawsone entrapment efficacy. The authors performed acridine orange/propidium iodide staining and flow cytometry to monitor apoptosis induction and cell cycle arrest. The expressions of apoptosis-related genes (BAX and BCL-2) were assessed by real time PCR. Nanoparticle antioxidative and antibacterial activities were examined by DPPH/ABTS scavenging assay, disk diffusion method, and minimum inhibitory concentration and minimum bactericidal concentration evaluation. The NPs were 229.65 nm, the encapsulation efficiency was 81%. The concentration of lawsone that exerts 50% cell growth inhibition (IC₅₀) against Panc-1 cells was calculated 118.4 μL. Apoptosis induction was evidenced by the increased number of orange cells and increased proportion of cells in G1-Sub phase respectively. Moreover, lawsone-loaded nanoparticle upregulated BAX gene expression, while downregulated BCL2expression, suggesting the activation of apoptotic pathway. The observed cytotoxic/apoptotic properties suggest that Lawson-loaded PLGA-FA-CS-NPs hold a great potential in pancreatic cancer treatment.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 5","pages":"425-437"},"PeriodicalIF":2.3,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/98/63/NBT2-17-425.PMC10374556.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9945593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteogenesis promotion by injectable methacryloylated gelatin containing psoralen and its bacteriostatic properties","authors":"Qi Zhang,&nbsp;Fuhang Chu,&nbsp;Yingjie Xu,&nbsp;Xiaonan Wu,&nbsp;Jie Yu,&nbsp;Beibei Cong,&nbsp;Yingtao Wu","doi":"10.1049/nbt2.12136","DOIUrl":"10.1049/nbt2.12136","url":null,"abstract":"<p>The treatment of periodontitis focuses on controlling the progression of inflammation, reducing plaque accumulation, and promoting bone tissue reconstruction. Among them, the reconstruction of irregular bone resorption caused by periodontitis is a long-standing challenge. At present, the local drug treatment of periodontitis is mainly anti-inflammatory and antibacterial drugs. In this study, psoralen (Pso), a Chinese herbal medicine with anti-inflammatory, antibacterial, and osteogenic effects, was selected for the local treatment of periodontitis. Meanwhile, an injectable methacrylate gelatin (GelMA) platform loading with Pso was constructed. Pso-GelMA had the properties of fluidity, light cohesion, self-healing, and slow release, which could be better used in the deep and narrow structure of the periodontal pocket, and greatly increased the effectiveness of local drug delivery. The pore size of Gelma hydrogel did not change after loading Pso by SEM. In vitro, Pso-GelMA effectively upregulated the expression of osteogenic genes and proteins, increased alkaline phosphatase activity, promoted the mineralisation of rat bone marrow mesenchymal stem cells (BMSCs) extracellular matrix, and had significant antibacterial effects on <i>Staphylococcus aureus</i> and <i>Fusobacterium nucle</i>atum. Therefore, Pso-GelMA has immense promise in the adjuvant treatment of periodontitis.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 4","pages":"376-386"},"PeriodicalIF":2.3,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f1/d8/NBT2-17-376.PMC10288355.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9698391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil membrane biomimetic delivery system (Ptdser-NM-Lipo/Fer-1) designed for targeting atherosclerosis therapy","authors":"Wei Li,&nbsp;Chang Liu,&nbsp;Sichuan Wang,&nbsp;Naifeng Liu","doi":"10.1049/nbt2.12137","DOIUrl":"10.1049/nbt2.12137","url":null,"abstract":"<p>Atherosclerosis is a progressive inflammatory disease characterised by excessive lipid accumulation and inflammatory cell infiltration and is the basis of most cardiovascular diseases and peripheral arterial diseases. Therefore, an effectively targeted delivery system is urgently needed to deliver ferroptosis-specific inhibitors to the site of arterial plaque and the inflammatory microenvironment. Inspired by the fact that neutrophils can be recruited to arterial plaques under the action of adhesion molecules and chemokines, the authors developed a neutrophil membrane hybrid liposome nano-mimetic system (Ptdser-NM-Lipo/Fer-1) that delivers Ferrostatin-1 (Fer-1) to the atherosclerotic plaque effectively, which is composed of Fer-1-loaded Ptdser-modified liposomes core and neutrophils shell. Fer-1 was released at the AS plaque site to remove reactive oxygen species (ROS) and improve the inflammatory microenvironment. In vitro ROS clearance experiments have shown that 50 μmol/ml Fer-1 can significantly remove ROS produced by H₂O₂-induced MOVAS cells and Ptdser-NM-Lipo/Fer-1 revealed a 3-fold increase in the inhibition rate of ROS than free Fer-1 in induced-RAW264.7, demonstrating its superior ROS-cleaning effect. Based on the interaction of adhesion molecules, such as vascular cell adhesion molecule 1, ICAM-1, P-selectin, E-selectin, and chemokines released in the inflamed site, the aorta in NM-Lipo-treated mice displayed 1.3-fold greater radiant efficiency than platelet membrane-Lipo-treated mice. Meanwhile, due to the modification of the Ptdser, the aorta in Ptdser-NM-Lipo/Fer-1-treated mice exhibited the highest fluorescence intensity, demonstrating its excellent targeting ability for atherosclerosis. Therefore, we present a specific formulation for the treatment of atherosclerosis with the potential for novel therapeutic uses.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 4","pages":"387-395"},"PeriodicalIF":2.3,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2.12137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9706115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthine oxidase inhibitory kinetics and mechanism of ellagic acid: In vitro, in silico and in vivo studies","authors":"Jianmin Chen,&nbsp;Zemin He,&nbsp;Sijin Yu,&nbsp;Xiaozhen Cai,&nbsp;Danhong Zhu,&nbsp;Yanhua Lin","doi":"10.1049/nbt2.12135","DOIUrl":"10.1049/nbt2.12135","url":null,"abstract":"<p>Ellagic acid (EA), which is widely distributed in many foods, has been found to possess inhibitory activity against xanthine oxidase (XO). However, there is ongoing debate about the difference in XO inhibitory activity between EA and allopurinol. Additionally, the inhibitory kinetics and mechanism of EA on XO are still unclear. Herein, the authors systematically studied the inhibitory effects of EA on XO. The authors’ findings showed that EA is a reversible inhibitor with mixed-type inhibition, and its inhibitory activity is weaker than allopurinol. Fluorescence quenching experiments suggested that the generation of EA-XO complex was exothermic and spontaneous. In silico analysis further confirmed that EA entered the XO catalytic centre. Furthermore, the authors verified the anti-hyperuricemia effect of EA in vivo. This study elucidates the inhibition kinetics and mechanism of EA on XO, and lays a theoretical foundation for the further development of drugs and functional foods containing EA for the treatment of hyperuricemia.</p>","PeriodicalId":13393,"journal":{"name":"IET nanobiotechnology","volume":"17 4","pages":"368-375"},"PeriodicalIF":2.3,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/nbt2.12135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9709414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}