Immunological Investigations最新文献_第8页

Targeting Macrophage Polarization in Infectious Diseases: M1/M2 Functional Profiles, Immune Signaling and Microbial Virulence Factors. 针对传染病中的巨噬细胞极化：M1/M2 功能图谱、免疫信号转导和微生物毒力因子。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-06-24 DOI: 10.1080/08820139.2024.2367682

Cláudio Daniel Cerdeira, Maísa R P L Brigagão

{"title":"Targeting Macrophage Polarization in Infectious Diseases: M1/M2 Functional Profiles, Immune Signaling and Microbial Virulence Factors.","authors":"Cláudio Daniel Cerdeira, Maísa R P L Brigagão","doi":"10.1080/08820139.2024.2367682","DOIUrl":"10.1080/08820139.2024.2367682","url":null,"abstract":"Introduction: An event of increasing interest during host-pathogen interactions is the polarization of patrolling/naive monocytes (MOs) into macrophage subsets (MФs). Therapeutic strategies aimed at modulating this event are under investigation.Methods: This review focuses on the mechanisms of induction/development and profile of MФs polarized toward classically proinflammatory (M1) or alternatively anti-inflammatory (M2) phenotypes in response to bacteria, fungi, parasites, and viruses.Results and discussion: It highlights nuclear, cytoplasmic, and cell surface receptors (pattern recognition receptors/PPRs), microenvironmental mediators, and immune signaling. MФs polarize into phenotypes: M1 MФs, activated by IFN-γ, pathogen-associated molecular patterns (PAMPs, e.g. lipopolysaccharide) and membrane-bound PPRs ligands (TLRs/CLRs ligands); or M2 MФs, induced by interleukins (ILs-4, -10 and -13), antigen-antibody complexes, and helminth PAMPs. Polarization toward M1 and M2 profiles evolve in a pathogen-specific manner, with or without canonicity, and can vary widely. Ultimately, this can result in varying degrees of host protection or more severe disease outcome. On the one hand, the host is driving effective MФs polarization (M1 or M2); but on the other hand, microorganisms may skew the polarization through virulence factors to increase pathogenicity. Cellular/genomic reprogramming also ensures plasticity of M1/M2 phenotypes. Because modulation of polarization can occur at multiple points, new insights and emerging perspectives may have clinical implications during the inflammation-to-resolution transition; translated into practical applications as for therapeutic/vaccine design target to boost microbicidal response (M1, e.g. triggering oxidative burst) with specifics PAMPs/IFN-γ or promote tissue repair (M2, increasing arginase activity) via immunotherapy.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1030-1091"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141446054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma Extracellular Vesicles Derived from Pediatric COVID-19 Patients Modulate Monocyte and T Cell Immune Responses Based on Disease Severity. 从小儿 COVID-19 患者体内提取的血浆细胞外小泡可根据疾病严重程度调节单核细胞和 T 细胞免疫反应。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1080/08820139.2024.2385992

Pınar Gur Cetinkaya, Irem Fatma Abras, Irem Evcili, Tugçe Yildirim, Yasemin Ceylan, Fehime Kara Eroglu, Başak Kayaoglu, Emre Mert İpekoglu, Aysegul Akarsu, Muzaffer Yıldırım, Tamer Kahraman, Ali Bülent Cengiz, Umit Murat Sahiner, Bulent Enis Sekerel, Yasemin Ozsurekci, Ozge Soyer, Ihsan Gursel

{"title":"Plasma Extracellular Vesicles Derived from Pediatric COVID-19 Patients Modulate Monocyte and T Cell Immune Responses Based on Disease Severity.","authors":"Pınar Gur Cetinkaya, Irem Fatma Abras, Irem Evcili, Tugçe Yildirim, Yasemin Ceylan, Fehime Kara Eroglu, Başak Kayaoglu, Emre Mert İpekoglu, Aysegul Akarsu, Muzaffer Yıldırım, Tamer Kahraman, Ali Bülent Cengiz, Umit Murat Sahiner, Bulent Enis Sekerel, Yasemin Ozsurekci, Ozge Soyer, Ihsan Gursel","doi":"10.1080/08820139.2024.2385992","DOIUrl":"10.1080/08820139.2024.2385992","url":null,"abstract":"Background: The COVID-19 pandemic has caused significant morbidity and mortality globally. The role of plasma-derived extracellular vesicles (EVs) in pediatric COVID-19 patients remains unclear.Methods: We isolated EVs from healthy controls (n = 13) and pediatric COVID-19 patients (n = 104) with varying severity during acute and convalescent phases using serial ultracentrifugation. EV effects on healthy PBMCs, naïve CD4+ T cells, and monocytes were assessed through in vitro assays, flow cytometry, and ELISA.Results: Our findings indicate that COVID-19 severity correlates with diverse immune responses. Severe acute cases exhibited increased cytokine levels, decreased IFNγ levels, and lower CD4+ T cell and monocyte counts, suggesting immunosuppression. EVs from severe acute patients stimulated healthy cells to express higher PDL1, increased Th2 and Treg cells, reduced IFNγ secretion, and altered Th1/Th17 ratios. Patient-derived EVs significantly reduced proinflammatory cytokine production by monocytes (p < .001 for mild, p = .0025 for severe cases) and decreased CD4+ T cell (p = .043) and monocyte (p = .033) populations in stimulated healthy PBMCs.Conclusion: This study reveals the complex relationship between immunological responses and EV-mediated effects, emphasizing the impact of COVID-19 severity. We highlight the potential role of plasma-derived EVs in early-stage immunosuppression in severe COVID-19 patients.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1141-1175"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protection of Astragalus Polysaccharide in BALB/C Mice during Brucella melitensis M5 Infection. 黄芪多糖对BALB/C小鼠M5布鲁氏菌感染的保护作用

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-29 DOI: 10.1080/08820139.2024.2380718

Yuanqiang Zheng, Yajing Chen, Jianlong Zhao, Meihua Wu, Ligao Bao, Dantong Zhao, Shuang Bai, Dongdong Di, Yanchun Shi

{"title":"The Protection of Astragalus Polysaccharide in BALB/C Mice during Brucella melitensis M5 Infection.","authors":"Yuanqiang Zheng, Yajing Chen, Jianlong Zhao, Meihua Wu, Ligao Bao, Dantong Zhao, Shuang Bai, Dongdong Di, Yanchun Shi","doi":"10.1080/08820139.2024.2380718","DOIUrl":"10.1080/08820139.2024.2380718","url":null,"abstract":"Introduction: Brucellosis is an important zoonosis worldwide, affecting humans and animals. There are no specific medicines available to treat brucellosis. Astragalus polysaccharide (APS) is derived from Astragalus membranaceus and exhibits impressive bioactivity, including anti-aging, anti-tumor, and immunomodulatory functions.Methods: Mice were intraperitoneally inoculated with Brucella melitensis M5 and then treated with APS intraperitoneally injection daily for 7 d.Results: Compared to the M5-infected group, the lower bacteria loads in the APS-treated groups were proved, especially at the acute stage of infection. APS treatment relieved splenomegaly, excess expressions of several pro-inflammatory cytokines (including CXCL1, IFN-γ, IL-1β, IL-2, IL-12p70, and TNF-α). The raised level of IL-4 was observed in APS-treated mice. APS contributed to raising the ratio of M1 macrophage and reducing the ratio of M2 macrophage in the blood.Discussion: The present study provides some evidence on the potential application of APS in controlling and treating brucellosis and should be further explored.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1102-1112"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increase in Mitochondrial Mass of Lymphocyte Subsets in Anti-MDA5 and TIF1-γ-Positive Dermatomyositis Patients. 抗 MDA5 和 TIF1-γ 阳性皮肌炎患者淋巴细胞亚群线粒体质量的增加

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-18 DOI: 10.1080/08820139.2024.2402824

Xiaomeng Li,Qingqing Ma,Yuan Huang,Linlin Cheng,Yongmei Liu,Haolong Li,Haoting Zhan,Fengchun Zhang,Yudong Liu,Yongzhe Li

{"title":"Increase in Mitochondrial Mass of Lymphocyte Subsets in Anti-MDA5 and TIF1-γ-Positive Dermatomyositis Patients.","authors":"Xiaomeng Li,Qingqing Ma,Yuan Huang,Linlin Cheng,Yongmei Liu,Haolong Li,Haoting Zhan,Fengchun Zhang,Yudong Liu,Yongzhe Li","doi":"10.1080/08820139.2024.2402824","DOIUrl":"https://doi.org/10.1080/08820139.2024.2402824","url":null,"abstract":"OBJECTIVESThe mitochondrial function in anti-MDA5 and TIF1-γ-positive dermatomyositis (DM) is relatively unknown. This study attempted to explore mitochondrial mass within the peripheral lymphocyte subsets of anti-MDA5 and TIF1-γ-positive DM.METHODSThis cross-sectional study enrolled 109 DM patients and 32 healthy controls (HCs). The mitochondrial mass of peripheral lymphocyte subsets was analyzed via flow cytometry using median fluorescence intensity assessment.RESULTSCompared with HCs, there was an abnormal change in peripheral lymphocyte subsets in anti-MDA5 and anti-TIF1-γ-positive DM patients. Anti-MDA5 and anti-TIF1-γ-positive DM patients also exhibited a significantly elevated mitochondrial mass in peripheral lymphocyte subsets. Furthermore, anti-MDA5 antibody levels were positively associated with the mitochondrial mass of most lymphocyte subsets in anti-MDA5-positive DM patients. Univariate logistic regression analysis indicated that the increased mitochondrial mass in some peripheral lymphocyte subsets was related to the occurrence of anti-MDA5-positive DM and presence of anti-MDA5 antibodies. Similar results were obtained in anti-TIF1-γ-positive DM patients.CONCLUSIONSAbnormal lymphocyte subset counts and percentages as well as altered mitochondrial mass in anti-MDA5 and TIF1-γ-positive DM patients were associated with anti-MDA5 and TIF1-γ antibodies. We believe that these results may provide novel mitochondria-based insights into DM pathogenesis.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"4 1","pages":"1-16"},"PeriodicalIF":2.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BMSCs-Derived Extracellular VesiclemiR-29a-3p Improved the Stability of Rat Myasthenia Gravis by Regulating Treg/Th17 Cells. BMSCs衍生的细胞外载体miR-29a-3p通过调节Treg/Th17细胞改善大鼠肌无力的稳定性

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-18 DOI: 10.1080/08820139.2024.2404629

Zhongben Tang,Meiqiu Chen,Chen Chen,Chao Fan,Jiaxian Huang

{"title":"BMSCs-Derived Extracellular VesiclemiR-29a-3p Improved the Stability of Rat Myasthenia Gravis by Regulating Treg/Th17 Cells.","authors":"Zhongben Tang,Meiqiu Chen,Chen Chen,Chao Fan,Jiaxian Huang","doi":"10.1080/08820139.2024.2404629","DOIUrl":"https://doi.org/10.1080/08820139.2024.2404629","url":null,"abstract":"INTRODUCTIONMyasthenia gravis (MG) is an autoimmune disorder. Microvesicle-derived miRNAs have been implicated in autoimmune diseases. However, the role of microvesicle-derived miR-29a-3p in MG remains poorly understood. This study aimed to investigate the therapeutic effect and mechanism of miR-29a-3p derived from stem cell microvesicles (MVs) on experimental autoimmune myasthenia gravis (EAMG) rats.METHODSEAMG was induced in rats by injection of the subunit of the rat nicotinic anti-acetylcholine receptor (AChR) R97-116 peptide.Besides the control group, EAMG rats were randomly allocated into the EAMG model group, MV group, MV-NC-agomir group, and MV- miR-29a-3p-agomir group.RESULTSOur results found that BMSCs-MV promoted miR-29a-3p expression in gastrocnemius of EAMG rats. Bone marrow mesenchymal stem cells (BMSCs) derived microvesicle miR-29a-3p improved the hanging ability and swimming time of EMGA rats and weakened the degree of muscle fiber atrophy. Furthermore, microvesicles from miR-29a-3p overexpressing BMSCs reduced the content of AchR-Ab in the serum of EAMG rats. BMSC-derived microvesicle miR-29a-3p further suppressed the expression of IFN-γ and enhanced the IL-4 and IL-10 in the serum of EAMG rats by restoring the Th17/Treg cells balance.DISCUSSIONBMSCs-derived microvesicle miR-29a-3p improved the stability of rat myasthenia gravis by regulating Treg/Th17 cells. It may be an effective treatment for MG.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"10 1","pages":"1-17"},"PeriodicalIF":2.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Inflammatory and Regulatory Cytokines in the Milk of Dairy Cows with Mastitis: A Comparative Study with Healthy Animals. 分析患有乳腺炎的奶牛乳汁中的炎性和调节性细胞因子：与健康动物的比较研究

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-17 DOI: 10.1080/08820139.2024.2404623

Elizabeta Lohova,Mara Pilmane,Ksenija Šerstņova,Ivars Melderis,Łukasz Gontar,Maksymilian Kochański,Andzelika Drutowska,Gergely Maróti,Beatriz Prieto-Simón

{"title":"Analysis of Inflammatory and Regulatory Cytokines in the Milk of Dairy Cows with Mastitis: A Comparative Study with Healthy Animals.","authors":"Elizabeta Lohova,Mara Pilmane,Ksenija Šerstņova,Ivars Melderis,Łukasz Gontar,Maksymilian Kochański,Andzelika Drutowska,Gergely Maróti,Beatriz Prieto-Simón","doi":"10.1080/08820139.2024.2404623","DOIUrl":"https://doi.org/10.1080/08820139.2024.2404623","url":null,"abstract":"Bovine mastitis remains a major problem in the global dairy cattle industry. The acute invasion of udder by pathogens induces innate immune response as the first defence mechanism in subclinical and clinical mastitis. The aim of the study was to determine inflammatory and regulatory cytokines IL-2, IL-4, TGF-β1, IL-17A, beta-defensin 3 and IL-10 and their potential changes in milk of dairy cows with subclinical and clinical mastitis, and to compare the findings with healthy animals. Milk samples from 15 holstein Friesian breed cows were used in the study. Cows were divided into three groups based on their health status (5 healthy, 5 subclinical and 5 clinical animals). All samples were tested using immunohistochemistry to evaluate IL-2, IL-4, IL-10, IL17A, TGF-β1 and β-Def 3 proteins. Expression of all proteins was detected in all milk samples. High expression of IL-2, IL-4, IL17A, TGF-β1 was detected in healthy cows' milk and in milk of cows with subclinical and clinical mastitis. However, expression of IL-10 and β-Def 3 in milk samples of healthy cows was significantly higher compared to the milk of cows with subclinical and clinical mastitis (p < .001). IL-10 and β-Def 3 can be considered as informative biomarkers in diagnosis of subclinical and clinical mastitis.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"210 1","pages":"1-25"},"PeriodicalIF":2.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invariant VD and DJ Motifs Define a Novel Class of Human Antibodies and TCRs Prototyped by antigen receptors of Dual-Expresser Lymphocytes. 由双表达淋巴细胞抗原受体原型定义的一类新型人类抗体和 TCR。

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-13 DOI: 10.1080/08820139.2024.2383736

Neha Majety,Rizwan Ahmed,Rafid Al-Hallaf,Prajita Paul,Adebola Giwa,Joseph Heinemann,Zainab Agha,Cherry Choong,Thomas Donner,Chunfa Jie,Abdel Rahim A Hamad

{"title":"Invariant VD and DJ Motifs Define a Novel Class of Human Antibodies and TCRs Prototyped by antigen receptors of Dual-Expresser Lymphocytes.","authors":"Neha Majety,Rizwan Ahmed,Rafid Al-Hallaf,Prajita Paul,Adebola Giwa,Joseph Heinemann,Zainab Agha,Cherry Choong,Thomas Donner,Chunfa Jie,Abdel Rahim A Hamad","doi":"10.1080/08820139.2024.2383736","DOIUrl":"https://doi.org/10.1080/08820139.2024.2383736","url":null,"abstract":"INTRODUCTIONDual-expressing lymphocytes (DEs) are unique immune cells that express both B cell receptors (BCRs, surface antibody) and T cell receptors (TCRs). In type 1 diabetes, DE antibodies are predominated by one antibody (x-mAb), an IgM monoclonal antibody with a germline-encoded CDR3 that recognizes self-reactive TCRs. We explored if x-mAb and its interacting TCRs have distinct structural features.METHODSUsing bioinformatics, we compared x-mAb and its most common interacting TCRαβ to billions of antigen receptor sequences to determine if they were unique or randomly generated.RESULTSX-mAb represents a unique class of human antibodies with a conserved CDR3 sequence (CARx1-4DTAMVYYFYDW), consisting of a fixed DJH motif (DTAMVYYFDYW) paired with various VH genes. A public TCRβ clonotype (CASSPGTEAFF) associated with x-mAb on DEs features two invariant segments, VβD (CASSPGT) and DJβ (PGTEAFF), key to two large families of public TCRβ clonotypes-CASSPGT-Jβx and CASSPGT-Jβx-formed by recombining the VβD motif with Jβ genes and the DJβ motif with Vβ genes. B cells also use CASSPGT as a VHD motif for public IGH clonotypes (CASSPGT-Jβx).DISCUSSIONDEs, unlike conventional T and B cells, use invariant motifs to create public antibodies and TCRs, a trait previously seen only in cartilaginous fish.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"24 1","pages":"1-16"},"PeriodicalIF":2.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Antiallergic Activity of Two Chemically/Enzymatically-Modified Natural Products Against Active Atopic and Systemic Anaphylaxes in CD1 Mice Models 两种经化学/酶解修饰的天然产品对 CD1 小鼠模型中活动性特应性和系统性过敏反应的潜在抗过敏活性

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-11 DOI: 10.1080/08820139.2024.2401551

Gamal Ramadan, Gehan Waheed, Hend A. Mohammed

引用次数: 0

Camel Whey Protein and Baicalein Suppressed Mast Cell Degranulation in Mice Models of IgE- and Non-IgE-Mediated Anaphylaxes: Potential Mechanisms on Downstream Cell Signaling of Mast Cells. 骆驼乳清蛋白和黄芩素抑制 IgE 和非 IgE 引起的过敏性休克小鼠模型中肥大细胞的脱颗粒作用：肥大细胞下游细胞信号传导的潜在机制。

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-11 DOI: 10.1080/08820139.2024.2400538

Hend Abbas,Gamal Badr,Gamal Ramadan,Sahar Sobhy Abd-Elhalem

{"title":"Camel Whey Protein and Baicalein Suppressed Mast Cell Degranulation in Mice Models of IgE- and Non-IgE-Mediated Anaphylaxes: Potential Mechanisms on Downstream Cell Signaling of Mast Cells.","authors":"Hend Abbas,Gamal Badr,Gamal Ramadan,Sahar Sobhy Abd-Elhalem","doi":"10.1080/08820139.2024.2400538","DOIUrl":"https://doi.org/10.1080/08820139.2024.2400538","url":null,"abstract":"INTRODUCTIONNovel treatments are being researched to develop more safe and effective protective medications for anaphylaxis. Camel whey protein (CWP) and baicalein (BAC, one of the major flavones) have multiple beneficial properties including anti-inflammatory and antioxidant activities.METHODSThe current study investigated/compared the therapeutic protection of repeated intragastric administration of CWP (100 mg/kg body weight, as an animal extract) and BAC (10 mg/kg body weight, as a plant extract), before the challenge with ovalbumin (OVA) or receiving the compound 48/80 (C48/80), against mice models for IgE-independent and dependent anaphylaxes. Besides, their effects on mast cells (MCs) downstream cell signaling were explored.RESULTSThe results revealed that CWP and BAC reduced the mortality rate, as compared with a MCs stabilizer \"sulfasalazine (SSZ, 100 mg/kg body weight, intraperitoneally),\" in both mice models. Furthermore, they prevented the MCs degranulation and significantly reduced (p < .05) lung tissue levels of cell signaling (p-AKT, p-ERK, and p-IκBα). Additionally, they decreased histamine, tryptase, leukotriene C4, prostaglandin D2, interleukin (IL)-4, and IL-10 levels in broncho-alveolar and peritoneal lavages in systemic anaphylaxis mice models. They also restored the stabilization of peritoneal MCs membrane in inverted light microscopy results accompanied by amelioration of the lung histology.DISCUSSIONThe present study provided evidence for the protective therapeutic effect of CWP and BAC against anaphylaxis. As a result, CWP and BAC may be used as preventative supplemented regimens for both non-vegetarian and vegetarian consumers to treat allergy through downregulation of MCs signal transduction pathways, and hence controlling the production of inflammatory mediators.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"36 1","pages":"1-18"},"PeriodicalIF":2.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142216581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Relationship Between Serum and Tissue Levels of IL-13 and TYK2 in Colorectal Cancer Patients. 结直肠癌患者血清和组织中 IL-13 与 TYK2 水平的关系

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-09 DOI: 10.1080/08820139.2024.2399581

Zahra Mozooni,Alireza Shahmohammadi,Nafiseh Golestani,Leyla Bahadorizadeh

{"title":"The Relationship Between Serum and Tissue Levels of IL-13 and TYK2 in Colorectal Cancer Patients.","authors":"Zahra Mozooni,Alireza Shahmohammadi,Nafiseh Golestani,Leyla Bahadorizadeh","doi":"10.1080/08820139.2024.2399581","DOIUrl":"https://doi.org/10.1080/08820139.2024.2399581","url":null,"abstract":"INTRODUCTIONColorectal cancer (CRC) is a third cause of death worldwide. The immune system plays a significant role in the tumor microenvironment and identifying its components involved in cancer development can aid in finding new biomarkers for prognosis, treatment monitoring, and immune-based therapies. Interleukin 13 (IL-13) is a cytokine produced by immune cells that has been implicated in tumor invasion, proliferation, and metastasis. Previous studies have shown that IL-13 causes the phosphorylation of Tyrosine kinase 2 (TYK2), which may contribute to the development and progression of cancer. This study investigated the levels expression of IL-13 and TYK2 in the tissue and serum of CRC patients and explored their possible association with pathological and clinical factors.METHODS105 patients with CRC and 105 healthy individuals were involved in the study. Tissue and blood samples were collected. The quantitative Real-Time PCR (qRT-PCR) technique was used to assess the expression levels of the IL-13 and TYK2 CRC tissue samples in comparison with the adjacent control tissue.RESULTThe expression levels of IL-13 were lower and TYK2 were found to be higher in CRC tissue compared to normal tissue. Additionally, serum levels of IL-13 were decreased in CRC patients while TYK2 levels were elevated. A significant negative correlation was found between the expression levels of IL-13 in both serum and tissue and the cancer stage.CONCLUSIONThese results suggest that IL-13 and TYKMay 2 play essential roles in CRC development and progression and may serve as potential biomarkers for early detection and treatment.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"220 1","pages":"1-14"},"PeriodicalIF":2.8,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142216586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0