The Relationship Between Serum and Tissue Levels of IL-13 and TYK2 in Colorectal Cancer Patients.

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2024-09-09 DOI:10.1080/08820139.2024.2399581

Zahra Mozooni,Alireza Shahmohammadi,Nafiseh Golestani,Leyla Bahadorizadeh

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Abstract

INTRODUCTION Colorectal cancer (CRC) is a third cause of death worldwide. The immune system plays a significant role in the tumor microenvironment and identifying its components involved in cancer development can aid in finding new biomarkers for prognosis, treatment monitoring, and immune-based therapies. Interleukin 13 (IL-13) is a cytokine produced by immune cells that has been implicated in tumor invasion, proliferation, and metastasis. Previous studies have shown that IL-13 causes the phosphorylation of Tyrosine kinase 2 (TYK2), which may contribute to the development and progression of cancer. This study investigated the levels expression of IL-13 and TYK2 in the tissue and serum of CRC patients and explored their possible association with pathological and clinical factors. METHODS 105 patients with CRC and 105 healthy individuals were involved in the study. Tissue and blood samples were collected. The quantitative Real-Time PCR (qRT-PCR) technique was used to assess the expression levels of the IL-13 and TYK2 CRC tissue samples in comparison with the adjacent control tissue. RESULT The expression levels of IL-13 were lower and TYK2 were found to be higher in CRC tissue compared to normal tissue. Additionally, serum levels of IL-13 were decreased in CRC patients while TYK2 levels were elevated. A significant negative correlation was found between the expression levels of IL-13 in both serum and tissue and the cancer stage. CONCLUSION These results suggest that IL-13 and TYKMay 2 play essential roles in CRC development and progression and may serve as potential biomarkers for early detection and treatment.

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结直肠癌患者血清和组织中 IL-13 与 TYK2 水平的关系

导言大肠癌（CRC）是全球第三大死因。免疫系统在肿瘤微环境中发挥着重要作用，确定其参与癌症发展的成分有助于找到新的生物标记物，用于预后判断、治疗监测和基于免疫的疗法。白细胞介素 13（IL-13）是一种由免疫细胞产生的细胞因子，与肿瘤的侵袭、增殖和转移有关。先前的研究表明，IL-13 会导致酪氨酸激酶 2（TYK2）磷酸化，这可能有助于癌症的发生和发展。本研究调查了 CRC 患者组织和血清中 IL-13 和 TYK2 的表达水平，并探讨了它们与病理和临床因素的可能关联。收集组织和血液样本。结果与正常组织相比，IL-13 在 CRC 组织中的表达水平较低，而 TYK2 在 CRC 组织中的表达水平较高。此外，CRC 患者血清中 IL-13 水平降低，而 TYK2 水平升高。这些结果表明，IL-13 和 TYKMay 2 在 CRC 的发生和发展过程中起着至关重要的作用，可作为早期检测和治疗的潜在生物标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.