BMSCs衍生的细胞外载体miR-29a-3p通过调节Treg/Th17细胞改善大鼠肌无力的稳定性

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2024-09-18 DOI:10.1080/08820139.2024.2404629

Zhongben Tang,Meiqiu Chen,Chen Chen,Chao Fan,Jiaxian Huang

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引用次数: 0

摘要

简介重症肌无力（MG）是一种自身免疫性疾病。微囊衍生的 miRNA 与自身免疫性疾病有关。然而，微囊衍生的miR-29a-3p在MG中的作用仍鲜为人知。本研究旨在探讨干细胞微囊（MVs）衍生的miR-29a-3p对实验性自身免疫性肌无力（EAMG）大鼠的治疗效果和机制。结果我们发现骨髓间充质干细胞-MV促进了EAMG大鼠腓肠肌中miR-29a-3p的表达。骨髓间充质干细胞（BMSCs）衍生的微囊miR-29a-3p改善了EMGA大鼠的悬垂能力和游泳时间，并减弱了肌纤维萎缩的程度。此外，miR-29a-3p过表达的BMSCs微囊还能降低EAMG大鼠血清中AchR-Ab的含量。BMSCs衍生的微囊miR-29a-3p通过恢复Th17/Treg细胞的平衡，进一步抑制了IFN-γ的表达，并增强了EAMG大鼠血清中IL-4和IL-10的表达。它可能是治疗肌萎缩症的一种有效方法。

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BMSCs-Derived Extracellular VesiclemiR-29a-3p Improved the Stability of Rat Myasthenia Gravis by Regulating Treg/Th17 Cells.

INTRODUCTION Myasthenia gravis (MG) is an autoimmune disorder. Microvesicle-derived miRNAs have been implicated in autoimmune diseases. However, the role of microvesicle-derived miR-29a-3p in MG remains poorly understood. This study aimed to investigate the therapeutic effect and mechanism of miR-29a-3p derived from stem cell microvesicles (MVs) on experimental autoimmune myasthenia gravis (EAMG) rats. METHODS EAMG was induced in rats by injection of the subunit of the rat nicotinic anti-acetylcholine receptor (AChR) R97-116 peptide.Besides the control group, EAMG rats were randomly allocated into the EAMG model group, MV group, MV-NC-agomir group, and MV- miR-29a-3p-agomir group. RESULTS Our results found that BMSCs-MV promoted miR-29a-3p expression in gastrocnemius of EAMG rats. Bone marrow mesenchymal stem cells (BMSCs) derived microvesicle miR-29a-3p improved the hanging ability and swimming time of EMGA rats and weakened the degree of muscle fiber atrophy. Furthermore, microvesicles from miR-29a-3p overexpressing BMSCs reduced the content of AchR-Ab in the serum of EAMG rats. BMSC-derived microvesicle miR-29a-3p further suppressed the expression of IFN-γ and enhanced the IL-4 and IL-10 in the serum of EAMG rats by restoring the Th17/Treg cells balance. DISCUSSION BMSCs-derived microvesicle miR-29a-3p improved the stability of rat myasthenia gravis by regulating Treg/Th17 cells. It may be an effective treatment for MG.

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.