Immunological Investigations最新文献_第7页

Characteristics of T-Cell Receptor Repertoire for Differential Response to Methotrexate Treatment for Rheumatoid Arthritis. 类风湿关节炎患者对甲氨蝶呤治疗差异反应的 T 细胞受体汇聚特征

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-14 DOI: 10.1080/08820139.2024.2381078

Taowa Zhao, Qian Zhang, Qinwen Wen, Shuyin Liu, Zitong Niu, Yang Qu, Yiting Wang, Qiaojiao Ding, Pengyao Wei, Lin Li, Tong Kong, Pan Fu, Sihua Qian, Kaizhe Wang, Xiudi Wu, Jianping Zheng

{"title":"Characteristics of T-Cell Receptor Repertoire for Differential Response to Methotrexate Treatment for Rheumatoid Arthritis.","authors":"Taowa Zhao, Qian Zhang, Qinwen Wen, Shuyin Liu, Zitong Niu, Yang Qu, Yiting Wang, Qiaojiao Ding, Pengyao Wei, Lin Li, Tong Kong, Pan Fu, Sihua Qian, Kaizhe Wang, Xiudi Wu, Jianping Zheng","doi":"10.1080/08820139.2024.2381078","DOIUrl":"10.1080/08820139.2024.2381078","url":null,"abstract":"Background: Methotrexate (MTX) serves as the initial treatment for rheumatoid arthritis (RA). However, a substantial proportion of RA patients, estimated between 30% and 50%, do not respond positively to MTX. While the T-cell receptor (TCR) is crucial for the immune response during RA, its role in differentiating MTX responsiveness has not been thoroughly investigated.Methods: This study used next-generation sequencing to analyze the TCR β-chain complementary determining region sequences in peripheral blood mononuclear cells obtained from RA patients before MTX treatment. This study aimed to compare the characteristics of the TCR repertoire between the MTX responder and non-responder groups.Results: The study identified a significant difference in the TRBV6-6 gene (p = .003) concerning MTX treatment response. Additionally, a significant difference was found in the number of \"3\" nucleotide deletions at the 5'Jdels site (p = .023) in the VDJ rearrangement.Conclusion: These findings suggest distinct TCR repertoire characteristics between MTX responder and non-responder groups among RA patients. This discovery offers new insights into understanding the variable responses of RA patients to MTX therapy.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1113-1124"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM). 原代小鼠骨髓衍生巨噬细胞（BMDM）的收集、分化、冷冻保存和复苏综合方案。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1080/08820139.2024.2382805

Abby M Luu, Kelly M Shepardson, Agnieszka Rynda-Apple

{"title":"A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM).","authors":"Abby M Luu, Kelly M Shepardson, Agnieszka Rynda-Apple","doi":"10.1080/08820139.2024.2382805","DOIUrl":"10.1080/08820139.2024.2382805","url":null,"abstract":"Background: The field of immunology has undoubtedly benefited from the in vitro use of cell lines for immunological studies; however, due to the \"immortal\" nature of many cell lines, they are not always the best model. Thus, direct collection and culture of primary cells from model organisms is a solution that many researchers utilize. To the best of our knowledge, there is not a singular protocol which encompasses the entire process of bone marrow cell collection through cryopreservation and resuscitation of cells from a murine model.Methods: Bone marrow cells were collected from mice with a C57BL6 genetic background. Cells were differentiated using L929 conditioned media. Cells were assessed using a combination of microscopy, differential staining, immunocytochemistry, and trypan blue. Results: Primary murine BMDMs that underwent cryopreservation followed by resuscitation retained a high degree of viability. Furthermore, these BMDMs retained on overall ability to clear S. aureus.Results: Primary murine BMDMs that underwent cryopreservation followed by resuscitation retained a high degree of viability. Furthermore, these BMDMs retained on overall ability to clear S. aureus.Conclusion: Crypopreserved and resuscitated primary murine BMDMs were viable and retained their pverall S. aureus clearance ability.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1001-1012"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11451725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predominance of CD137⁺ And TNF-α Expressing CD8⁺ Central Memory T Cells in Mild COVID-19 Recovered Patients Upon SARS-CoV-2 Re-Exposure. 再次接触SARS-CoV-2病毒后，轻度COVID-19康复者体内CD137+和TNF-α表达的CD8+中央记忆T细胞占主导地位

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-07-12 DOI: 10.1080/08820139.2024.2376003

Rephany Fonseca Peixoto, Pedro Henrique de Sousa Palmeira, Bárbara Guimarães Csordas, Luiz Henrique Agra Cavalcante-Silva, Arthur Gomes de Andrade, Isac Almeida de Medeiros, Fátima de Lourdes Assunção Araújo de Azevedo, Robson Cavalcante Veras, Daniele Janebro, Ian P G Do Amaral, Tatjana Souza Lima Keesen

{"title":"Predominance of CD137+ And TNF-α Expressing CD8+ Central Memory T Cells in Mild COVID-19 Recovered Patients Upon SARS-CoV-2 Re-Exposure.","authors":"Rephany Fonseca Peixoto, Pedro Henrique de Sousa Palmeira, Bárbara Guimarães Csordas, Luiz Henrique Agra Cavalcante-Silva, Arthur Gomes de Andrade, Isac Almeida de Medeiros, Fátima de Lourdes Assunção Araújo de Azevedo, Robson Cavalcante Veras, Daniele Janebro, Ian P G Do Amaral, Tatjana Souza Lima Keesen","doi":"10.1080/08820139.2024.2376003","DOIUrl":"10.1080/08820139.2024.2376003","url":null,"abstract":"Introduction: Memory CD8+ T cells are essential for long-term immune protection in viral infections, including COVID-19.Methods: This study examined the responses of CD8+ TEM, TEMRA, and TCM subsets from unvaccinated individuals who had recovered from mild and severe COVID-19 by flow cytometry.Results and discussion: The peptides triggered a higher frequency of CD8+ TCM cells in the recovered mild group. CD8+ TCM and TEM cells showed heterogeneity in CD137 expression between evaluated groups. In addition, a predominance of CD137 expression in naïve CD8+ T cells, TCM, and TEM was observed in the mild recovered group when stimulated with peptides. Furthermore, CD8+ TCM and TEM cell subsets from mild recovered volunteers had higher TNF-α expression. In contrast, the expression partner of IFN-γ, IL-10, and IL-17 indicated an antiviral signature by CD8+ TEMRA cells. These findings underscore the distinct functional capabilities of each memory T cell subset in individuals who have recovered from COVID-19 upon re-exposure to SARS-CoV-2 antigens.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1092-1101"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Neuro-Immune Interactions in the Pathology and Pathogenesis of Allergic Rhinitis. 神经免疫相互作用在过敏性鼻炎病理和发病机制中的作用。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-07-23 DOI: 10.1080/08820139.2024.2382792

Ya-An Lan, Jia-Xi Guo, Min-Hua Yao, Yi-Ting Kang, Zi-Rui Liao, Yu-Hong Jing

引用次数: 0

Targeting Macrophage Polarization in Infectious Diseases: M1/M2 Functional Profiles, Immune Signaling and Microbial Virulence Factors. 针对传染病中的巨噬细胞极化：M1/M2 功能图谱、免疫信号转导和微生物毒力因子。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-06-24 DOI: 10.1080/08820139.2024.2367682

Cláudio Daniel Cerdeira, Maísa R P L Brigagão

{"title":"Targeting Macrophage Polarization in Infectious Diseases: M1/M2 Functional Profiles, Immune Signaling and Microbial Virulence Factors.","authors":"Cláudio Daniel Cerdeira, Maísa R P L Brigagão","doi":"10.1080/08820139.2024.2367682","DOIUrl":"10.1080/08820139.2024.2367682","url":null,"abstract":"Introduction: An event of increasing interest during host-pathogen interactions is the polarization of patrolling/naive monocytes (MOs) into macrophage subsets (MФs). Therapeutic strategies aimed at modulating this event are under investigation.Methods: This review focuses on the mechanisms of induction/development and profile of MФs polarized toward classically proinflammatory (M1) or alternatively anti-inflammatory (M2) phenotypes in response to bacteria, fungi, parasites, and viruses.Results and discussion: It highlights nuclear, cytoplasmic, and cell surface receptors (pattern recognition receptors/PPRs), microenvironmental mediators, and immune signaling. MФs polarize into phenotypes: M1 MФs, activated by IFN-γ, pathogen-associated molecular patterns (PAMPs, e.g. lipopolysaccharide) and membrane-bound PPRs ligands (TLRs/CLRs ligands); or M2 MФs, induced by interleukins (ILs-4, -10 and -13), antigen-antibody complexes, and helminth PAMPs. Polarization toward M1 and M2 profiles evolve in a pathogen-specific manner, with or without canonicity, and can vary widely. Ultimately, this can result in varying degrees of host protection or more severe disease outcome. On the one hand, the host is driving effective MФs polarization (M1 or M2); but on the other hand, microorganisms may skew the polarization through virulence factors to increase pathogenicity. Cellular/genomic reprogramming also ensures plasticity of M1/M2 phenotypes. Because modulation of polarization can occur at multiple points, new insights and emerging perspectives may have clinical implications during the inflammation-to-resolution transition; translated into practical applications as for therapeutic/vaccine design target to boost microbicidal response (M1, e.g. triggering oxidative burst) with specifics PAMPs/IFN-γ or promote tissue repair (M2, increasing arginase activity) via immunotherapy.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1030-1091"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141446054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma Extracellular Vesicles Derived from Pediatric COVID-19 Patients Modulate Monocyte and T Cell Immune Responses Based on Disease Severity. 从小儿 COVID-19 患者体内提取的血浆细胞外小泡可根据疾病严重程度调节单核细胞和 T 细胞免疫反应。

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1080/08820139.2024.2385992

Pınar Gur Cetinkaya, Irem Fatma Abras, Irem Evcili, Tugçe Yildirim, Yasemin Ceylan, Fehime Kara Eroglu, Başak Kayaoglu, Emre Mert İpekoglu, Aysegul Akarsu, Muzaffer Yıldırım, Tamer Kahraman, Ali Bülent Cengiz, Umit Murat Sahiner, Bulent Enis Sekerel, Yasemin Ozsurekci, Ozge Soyer, Ihsan Gursel

{"title":"Plasma Extracellular Vesicles Derived from Pediatric COVID-19 Patients Modulate Monocyte and T Cell Immune Responses Based on Disease Severity.","authors":"Pınar Gur Cetinkaya, Irem Fatma Abras, Irem Evcili, Tugçe Yildirim, Yasemin Ceylan, Fehime Kara Eroglu, Başak Kayaoglu, Emre Mert İpekoglu, Aysegul Akarsu, Muzaffer Yıldırım, Tamer Kahraman, Ali Bülent Cengiz, Umit Murat Sahiner, Bulent Enis Sekerel, Yasemin Ozsurekci, Ozge Soyer, Ihsan Gursel","doi":"10.1080/08820139.2024.2385992","DOIUrl":"10.1080/08820139.2024.2385992","url":null,"abstract":"Background: The COVID-19 pandemic has caused significant morbidity and mortality globally. The role of plasma-derived extracellular vesicles (EVs) in pediatric COVID-19 patients remains unclear.Methods: We isolated EVs from healthy controls (n = 13) and pediatric COVID-19 patients (n = 104) with varying severity during acute and convalescent phases using serial ultracentrifugation. EV effects on healthy PBMCs, naïve CD4+ T cells, and monocytes were assessed through in vitro assays, flow cytometry, and ELISA.Results: Our findings indicate that COVID-19 severity correlates with diverse immune responses. Severe acute cases exhibited increased cytokine levels, decreased IFNγ levels, and lower CD4+ T cell and monocyte counts, suggesting immunosuppression. EVs from severe acute patients stimulated healthy cells to express higher PDL1, increased Th2 and Treg cells, reduced IFNγ secretion, and altered Th1/Th17 ratios. Patient-derived EVs significantly reduced proinflammatory cytokine production by monocytes (p < .001 for mild, p = .0025 for severe cases) and decreased CD4+ T cell (p = .043) and monocyte (p = .033) populations in stimulated healthy PBMCs.Conclusion: This study reveals the complex relationship between immunological responses and EV-mediated effects, emphasizing the impact of COVID-19 severity. We highlight the potential role of plasma-derived EVs in early-stage immunosuppression in severe COVID-19 patients.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1141-1175"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protection of Astragalus Polysaccharide in BALB/C Mice during Brucella melitensis M5 Infection. 黄芪多糖对BALB/C小鼠M5布鲁氏菌感染的保护作用

IF 2.9 4区医学

Immunological Investigations Pub Date : 2024-10-01 Epub Date: 2024-08-29 DOI: 10.1080/08820139.2024.2380718

Yuanqiang Zheng, Yajing Chen, Jianlong Zhao, Meihua Wu, Ligao Bao, Dantong Zhao, Shuang Bai, Dongdong Di, Yanchun Shi

{"title":"The Protection of Astragalus Polysaccharide in BALB/C Mice during Brucella melitensis M5 Infection.","authors":"Yuanqiang Zheng, Yajing Chen, Jianlong Zhao, Meihua Wu, Ligao Bao, Dantong Zhao, Shuang Bai, Dongdong Di, Yanchun Shi","doi":"10.1080/08820139.2024.2380718","DOIUrl":"10.1080/08820139.2024.2380718","url":null,"abstract":"Introduction: Brucellosis is an important zoonosis worldwide, affecting humans and animals. There are no specific medicines available to treat brucellosis. Astragalus polysaccharide (APS) is derived from Astragalus membranaceus and exhibits impressive bioactivity, including anti-aging, anti-tumor, and immunomodulatory functions.Methods: Mice were intraperitoneally inoculated with Brucella melitensis M5 and then treated with APS intraperitoneally injection daily for 7 d.Results: Compared to the M5-infected group, the lower bacteria loads in the APS-treated groups were proved, especially at the acute stage of infection. APS treatment relieved splenomegaly, excess expressions of several pro-inflammatory cytokines (including CXCL1, IFN-γ, IL-1β, IL-2, IL-12p70, and TNF-α). The raised level of IL-4 was observed in APS-treated mice. APS contributed to raising the ratio of M1 macrophage and reducing the ratio of M2 macrophage in the blood.Discussion: The present study provides some evidence on the potential application of APS in controlling and treating brucellosis and should be further explored.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"1102-1112"},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increase in Mitochondrial Mass of Lymphocyte Subsets in Anti-MDA5 and TIF1-γ-Positive Dermatomyositis Patients. 抗 MDA5 和 TIF1-γ 阳性皮肌炎患者淋巴细胞亚群线粒体质量的增加

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-18 DOI: 10.1080/08820139.2024.2402824

Xiaomeng Li,Qingqing Ma,Yuan Huang,Linlin Cheng,Yongmei Liu,Haolong Li,Haoting Zhan,Fengchun Zhang,Yudong Liu,Yongzhe Li

{"title":"Increase in Mitochondrial Mass of Lymphocyte Subsets in Anti-MDA5 and TIF1-γ-Positive Dermatomyositis Patients.","authors":"Xiaomeng Li,Qingqing Ma,Yuan Huang,Linlin Cheng,Yongmei Liu,Haolong Li,Haoting Zhan,Fengchun Zhang,Yudong Liu,Yongzhe Li","doi":"10.1080/08820139.2024.2402824","DOIUrl":"https://doi.org/10.1080/08820139.2024.2402824","url":null,"abstract":"OBJECTIVESThe mitochondrial function in anti-MDA5 and TIF1-γ-positive dermatomyositis (DM) is relatively unknown. This study attempted to explore mitochondrial mass within the peripheral lymphocyte subsets of anti-MDA5 and TIF1-γ-positive DM.METHODSThis cross-sectional study enrolled 109 DM patients and 32 healthy controls (HCs). The mitochondrial mass of peripheral lymphocyte subsets was analyzed via flow cytometry using median fluorescence intensity assessment.RESULTSCompared with HCs, there was an abnormal change in peripheral lymphocyte subsets in anti-MDA5 and anti-TIF1-γ-positive DM patients. Anti-MDA5 and anti-TIF1-γ-positive DM patients also exhibited a significantly elevated mitochondrial mass in peripheral lymphocyte subsets. Furthermore, anti-MDA5 antibody levels were positively associated with the mitochondrial mass of most lymphocyte subsets in anti-MDA5-positive DM patients. Univariate logistic regression analysis indicated that the increased mitochondrial mass in some peripheral lymphocyte subsets was related to the occurrence of anti-MDA5-positive DM and presence of anti-MDA5 antibodies. Similar results were obtained in anti-TIF1-γ-positive DM patients.CONCLUSIONSAbnormal lymphocyte subset counts and percentages as well as altered mitochondrial mass in anti-MDA5 and TIF1-γ-positive DM patients were associated with anti-MDA5 and TIF1-γ antibodies. We believe that these results may provide novel mitochondria-based insights into DM pathogenesis.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"4 1","pages":"1-16"},"PeriodicalIF":2.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BMSCs-Derived Extracellular VesiclemiR-29a-3p Improved the Stability of Rat Myasthenia Gravis by Regulating Treg/Th17 Cells. BMSCs衍生的细胞外载体miR-29a-3p通过调节Treg/Th17细胞改善大鼠肌无力的稳定性

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-18 DOI: 10.1080/08820139.2024.2404629

Zhongben Tang,Meiqiu Chen,Chen Chen,Chao Fan,Jiaxian Huang

{"title":"BMSCs-Derived Extracellular VesiclemiR-29a-3p Improved the Stability of Rat Myasthenia Gravis by Regulating Treg/Th17 Cells.","authors":"Zhongben Tang,Meiqiu Chen,Chen Chen,Chao Fan,Jiaxian Huang","doi":"10.1080/08820139.2024.2404629","DOIUrl":"https://doi.org/10.1080/08820139.2024.2404629","url":null,"abstract":"INTRODUCTIONMyasthenia gravis (MG) is an autoimmune disorder. Microvesicle-derived miRNAs have been implicated in autoimmune diseases. However, the role of microvesicle-derived miR-29a-3p in MG remains poorly understood. This study aimed to investigate the therapeutic effect and mechanism of miR-29a-3p derived from stem cell microvesicles (MVs) on experimental autoimmune myasthenia gravis (EAMG) rats.METHODSEAMG was induced in rats by injection of the subunit of the rat nicotinic anti-acetylcholine receptor (AChR) R97-116 peptide.Besides the control group, EAMG rats were randomly allocated into the EAMG model group, MV group, MV-NC-agomir group, and MV- miR-29a-3p-agomir group.RESULTSOur results found that BMSCs-MV promoted miR-29a-3p expression in gastrocnemius of EAMG rats. Bone marrow mesenchymal stem cells (BMSCs) derived microvesicle miR-29a-3p improved the hanging ability and swimming time of EMGA rats and weakened the degree of muscle fiber atrophy. Furthermore, microvesicles from miR-29a-3p overexpressing BMSCs reduced the content of AchR-Ab in the serum of EAMG rats. BMSC-derived microvesicle miR-29a-3p further suppressed the expression of IFN-γ and enhanced the IL-4 and IL-10 in the serum of EAMG rats by restoring the Th17/Treg cells balance.DISCUSSIONBMSCs-derived microvesicle miR-29a-3p improved the stability of rat myasthenia gravis by regulating Treg/Th17 cells. It may be an effective treatment for MG.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"10 1","pages":"1-17"},"PeriodicalIF":2.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Inflammatory and Regulatory Cytokines in the Milk of Dairy Cows with Mastitis: A Comparative Study with Healthy Animals. 分析患有乳腺炎的奶牛乳汁中的炎性和调节性细胞因子：与健康动物的比较研究

IF 2.8 4区医学

Immunological Investigations Pub Date : 2024-09-17 DOI: 10.1080/08820139.2024.2404623

Elizabeta Lohova,Mara Pilmane,Ksenija Šerstņova,Ivars Melderis,Łukasz Gontar,Maksymilian Kochański,Andzelika Drutowska,Gergely Maróti,Beatriz Prieto-Simón

{"title":"Analysis of Inflammatory and Regulatory Cytokines in the Milk of Dairy Cows with Mastitis: A Comparative Study with Healthy Animals.","authors":"Elizabeta Lohova,Mara Pilmane,Ksenija Šerstņova,Ivars Melderis,Łukasz Gontar,Maksymilian Kochański,Andzelika Drutowska,Gergely Maróti,Beatriz Prieto-Simón","doi":"10.1080/08820139.2024.2404623","DOIUrl":"https://doi.org/10.1080/08820139.2024.2404623","url":null,"abstract":"Bovine mastitis remains a major problem in the global dairy cattle industry. The acute invasion of udder by pathogens induces innate immune response as the first defence mechanism in subclinical and clinical mastitis. The aim of the study was to determine inflammatory and regulatory cytokines IL-2, IL-4, TGF-β1, IL-17A, beta-defensin 3 and IL-10 and their potential changes in milk of dairy cows with subclinical and clinical mastitis, and to compare the findings with healthy animals. Milk samples from 15 holstein Friesian breed cows were used in the study. Cows were divided into three groups based on their health status (5 healthy, 5 subclinical and 5 clinical animals). All samples were tested using immunohistochemistry to evaluate IL-2, IL-4, IL-10, IL17A, TGF-β1 and β-Def 3 proteins. Expression of all proteins was detected in all milk samples. High expression of IL-2, IL-4, IL17A, TGF-β1 was detected in healthy cows' milk and in milk of cows with subclinical and clinical mastitis. However, expression of IL-10 and β-Def 3 in milk samples of healthy cows was significantly higher compared to the milk of cows with subclinical and clinical mastitis (p < .001). IL-10 and β-Def 3 can be considered as informative biomarkers in diagnosis of subclinical and clinical mastitis.","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":"210 1","pages":"1-25"},"PeriodicalIF":2.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0