Indian Journal of Microbiology最新文献

{"title":"A Comprehensive Review of Molecular Mechanisms Leading to the Emergence of Multidrug Resistance in Bacteria","authors":"Vandana Jhalora, Renu Bist","doi":"10.1007/s12088-024-01384-6","DOIUrl":"https://doi.org/10.1007/s12088-024-01384-6","url":null,"abstract":"<p>Multidrug resistance (MDR) in bacteria poses a serious global health threat, compromising the effectiveness of antibiotics. MDR causes approximately 700,000 deaths annually, with MDR tuberculosis alone claiming 230,000 lives. While bacteria inherently possess intrinsic resistance, acquired resistance stands out as the primary culprit in MDR development. Acquired resistance mechanisms mediated by the bacterial cell wall, nucleic acids, and proteins play a pivotal role in the genesis of MDR. Bacteria can modify their cell wall structure, produce resistant enzymes, exhibit mutations in antibiotic-targeted genes, and acquire resistant genes through horizontal gene transfer. Bacteria can produce proteins that act as enzymes, chemically modifying or directly degrading the antibiotic molecules, leading to the loss of their functionality. Apart from these mechanisms, biofilms also play a pivotal role in MDR expansion. Despite the development of several antibiotics since the discovery of penicillin, continuous structural and molecular modifications in bacteria render these antibiotics ineffective against MDR. The most recent approaches such as clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (CRISPR-Cas), nanotechnology, a combination of CRISPR-Cas, and nanoparticles, show promise in treating MDR. Thus, this review delves deep into the molecular mechanisms of MDR, emphasizing the limitations of current antibiotics due to bacterial evolution and highlighting current strategies in the fight against MDR bacteria. This will drive comprehensive research to uncover additional resistance mechanisms and develop innovative strategies to combat resistant bacteria effectively.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"31 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142227469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Remediation of Polycyclic Aromatic Hydrocarbons in Soil Through Fungal Delignification Strategy and Organic Waste Amendment: A Review","authors":"Victor Taghoghor Omoni, Paul Olusegun Bankole, Kirk Taylor Semple, Adesola Samson Ojo, Cynthia Ibeto, Stephen Efe Okekporo, Idorenyin Ambrose Harrison","doi":"10.1007/s12088-024-01383-7","DOIUrl":"https://doi.org/10.1007/s12088-024-01383-7","url":null,"abstract":"<p>Nutrient-limited soils from growing global contamination with polycyclic aromatic hydrocarbons (PAHs) and the massive organic waste generation from agro-based and food industries have raised more demand for exploring and recycling the latter as sustainable, cost-effective, and green nutrient-rich sources for soil amendment. To further enhanced the potentials of these substrates in soil, immobilisation or biological pre-treatment techniques using fungi are employed. The white-rot fungi- basidiomycetes, are the most widely researched and efficient organisms to perform these functions because of their high lignin-degrading ability for organic materials, such as corn cob, straws, spent brewery grains, sugarcane bagasse, etc. This review addresses the importance of organic amendment to enhance the biodegradation efficiency of PAH from contaminated soils and it also highlights various biological techniques for improving PAH biodegradation using organic waste materials and white-rot basidiomycetes. This review will also show a better understanding of the concepts of fungal immobilisation and pre-treatment for PAH degradation in soil and show their insights as feasible and optimise techniques for developing remedial strategies for contaminated soils.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"5 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gliadin Encapsulated Procyanidolic Oligomers Nanoformulation: Aggrandized Antimicrobial, Antioxidant as Well as Anticancer Potential Makes it an Effective Therapeutic Modality for Lung Carcinoma","authors":"Sant Lal, Monika Kataria, Ruma Rani, Neeraj Dilbaghi","doi":"10.1007/s12088-024-01380-w","DOIUrl":"https://doi.org/10.1007/s12088-024-01380-w","url":null,"abstract":"<p>Lung cancer is responsible for highest mortality rates among both men and women globally. There is a need of effective and safe treatment modality that specifically targets cancer cells and doesn’t not harm healthy cells. Procyanidolic oligomers (PCOs) is a natural antioxidant found in grape seed, acts as medicament for the treatment of various disease which are linked with oxidative stress specifically cancer. The aim of the current study was to synthesize highly stable gliadin encapsulated PCOs nanoformulation (PCO-NF). The PCOs nanoformulation (PCO-NF) showed spherical morphology (17.18–76.56 nm) with an average particle size 49.37 ± 22.17 nm diameter in electron microscopy and stable zeta potential i.e. − 23.1 mV and low polydispersity index i.e. 0.246 in dynamic light scattering (DLS). The therapeutic efficacy of PCO-NF has been evaluated through antioxidative, anticancer as well as antimicrobial studies. Antioxidant potency was assessed using DPPH scavenging assay and it was observed that IC₅₀ of PCO-NF (9.02 µg/ml) was even lower than that of Vitamin C (10.22 µg/ml) as well as pure PCOs. The MTT assay was performed for the comparative anticancer activity of pure PCOs and PCO-NF against SK-MES-1 lung cancer cell line. It was found that PCO-NF significantly inhibited the lung cancer cells at all tested concentration. Moreover at 125 µg/ml concentration the PCO-NF showed 31.54% cell viability which was even lesser than the standard anticancer drug. The antimicrobial efficacy of PCO-NF was assessed against gram-positive and gram-negative bacteria. An augmented antimicrobial, antioxidant and anticancer potential was observed due to nanoencapsulation of PCOs.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"4 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphasic Characterization of Ca. Methylomicrobium oryzae: A Methanotroph Isolated from Rice Fields","authors":"Monali C. Rahalkar, Kumal Khatri, Pranitha Pandit, Jyoti A. Mohite","doi":"10.1007/s12088-024-01381-9","DOIUrl":"https://doi.org/10.1007/s12088-024-01381-9","url":null,"abstract":"<p>Global warming is the current threat to mankind and methane is the second most important greenhouse gas. Methanotrophs represent a group of bacteria that oxidize methane and are, therefore, environmentally very important. The cultivation of methanotrophs poses a challenge due to their specific cultivation conditions. Rice fields are important habitats where methanotrophs play a vital role in methane mitigation. We recently cultured <i>Methylomicrobium</i> strain RS1 from a rice field in India and briefly suggested it to be a member of putative novel species (<i>Ca.</i> Methylomicrobium oryzae) using draft genome-based ANI and DDH parameters (Rahalkar et al. in Front Microbiol 12:1–15, 2021). This culture presented challenges in terms of weak growth and inability to get cryopreserved. In the present work, we carried out the polyphasic characterization of this novel species which encompasses its chemotaxonomic, metabolic properties and salient genome features. <i>Ca.</i> Methylomicrobium oryzae is the first cultured methanotroph to be described from rice field habitats and could be an essential player in these ecosystems. It awaits further studies. <i>Methylomicrobium</i> sequences have been reported from rice fields but there were no reports of cultivation of members from this group, and this is the first report. Due to its growth challenges and fastidious nature of growth, inability to be cryo-preserved, and additionally due to legislations imposed on countries including India, the culture cannot be deposited in two international culture collections in other foreign countries. Within India, <i>Ca</i>. Methylomicrobium oryzae RS1 is maintained in our laboratory as a live culture at 4 °C and at room temperature (25–30 °C) by subculturing after intervals. Our laboratory is part of the WDCM approved culture collection MCM, and the culture is registered as MCMB-1473. It is also deposited in another internationally recognized culture collection: NCMR, Pune with the accession number MCC-4197. The draft genome of <i>Ca</i>. Methylomicrobium oryzae RS1 is available as JAERVK01.1.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"72 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Genome Sequencing of Carbapenem-Resistant Enterobacter hormaechei Isolated from Thiruvananthapuram, Kerala (India)","authors":"Parvathi Vaikkathillam, Praveen Kumar, S. Manjusree, Minsa Mini, Devi Jayakumar, Amjesh Revikumar","doi":"10.1007/s12088-024-01377-5","DOIUrl":"https://doi.org/10.1007/s12088-024-01377-5","url":null,"abstract":"<p>The rapid emergence of carbapenem-resistant Enterobacteriaceae is a critical public health issue across the globe. Widespread antibiotic resistance to multiple drugs has been reported in clinical isolates of <i>Enterobacter hormaechei</i>. In the present investigation, we report the whole genome sequencing (WGS) of a multi-drug resistant (MDR), clinical strain of <i>E. hormaechei</i>, GC26 isolated from Thiruvananthapuram, Kerala (India). Using the Oxford Nanopore sequencing platform, we identified key drug-resistance genes (<i>bla</i>, <i>tet</i>, <i>armA</i>, <i>qnrB</i>, and <i>fos</i>) and virulence genes (<i>fimD</i>, <i>csgD</i>, <i>csgE</i>, <i>csgF</i>, and <i>csgG</i>) in GC26. Multi-Locus Sequence Typing (MLST) analysis of GC26 revealed a novel sequence type (ST*ba28). Phylogenetic analysis showed that the GC26 genome occupies a distinct branch within the <i>Enterobacter</i> genus, closely related to Indian strains of <i>E. hormaechei</i> rather than the global population. This study provides valuable insights into the genetic basis of multidrug resistance in <i>E. hormaechei</i>. These findings underscore the critical need for continuous monitoring and robust epidemiological surveillance of MDR <i>E. hormaechei</i> strains.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"61 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Growth-Promoting Endophytic Bacteria on Ginger Plant Growth","authors":"Dilfuza Jabborova, Kakhramon Davranov, Zafarjon Jabbarov, Yuriy Enakiev, Tokhtasin Abdrakhmanov, Rahul Datta, Sachidanand Singh, Mohammad Shah Jahan, Sezai Ercisli, Namita Singh","doi":"10.1007/s12088-024-01379-3","DOIUrl":"https://doi.org/10.1007/s12088-024-01379-3","url":null,"abstract":"<p>Phytohormones has produced by endophytic bacteria promote the development and yield in plants. To investigate the role of endophytic bacteria on the soil properties and the growth of ginger, a net house experiment was carried. The experiment was used four treatments (Control, <i>Bacillus subtilis</i> IGPEB 34, <i>Bacillus pumilis</i> IGPEB 37, and <i>Bacillus altitudinis</i> IGPEB 20). <i>Bacillus subtilis</i> IGPEB 34 and<i> B. pumilis</i> IGPEB 37 significantly increased plant growth (the plant height, leaf number, leaf length, and leaf width) as compared to the control treatment. Moreover, inoculation of <i>B. subtilis</i> IGPEB 34 treatment significantly enhanced the total chlorophyll, chlorophyll a, chlorophyll b and carotenoid contents. In comparison to the control, inoculation <i>B. subtilis</i> IGPEB 34 treatment significantly enhanced the catalase, invertase and phosphatase activities in soil, the total phosphorus (P), potassium (K), carbon (C), and humus contents in the soil. The results concluded that <i>B. subtilis</i> IGPEB 34 strain positively influence the soil nutrients, along with morphological and physiological characteristics of ginger.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"21 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rising Threats of Viral Infections: Exploring Probiotics as Antiviral Agents","authors":"Haneef Mohammad Bhat, Ruqeya Nazir, Zahid Amin Kashoo","doi":"10.1007/s12088-024-01378-4","DOIUrl":"https://doi.org/10.1007/s12088-024-01378-4","url":null,"abstract":"<p>Viral infections are the most common etiological agents behind a wide range of human illnesses, with significant and widespread effects on human health. Vaccines have been developed to combat viral infectious diseases in various ways. However, the high rate of mutation in viruses, specifically RNA viruses, makes vaccines and medications for viral infectious diseases ineffective. Meanwhile, published research continues to offer insight into the efficacy of probiotics as antiviral agents. Various clinical findings reveal those specific probiotic strains aid in the prevention of viral and bacterial infections. Probiotics have been used to prevent and treat common viral infections such as rotavirus, coronavirus, hepatitis, human papillomavirus, human immunodeficiency virus, and herpes simplex viruses etc. The studies compiled in this review demonstrate the value of probiotics in the treatment and prevention of several viral infections using in vitro and in vivo trials in both experimental animals and humans and also provide perspectives on probiotics' probable antiviral mechanisms. Although the initial findings are promising, the current research is limited by small sample sizes, short study durations, and a lack of diversity in population groups. Consequently, further research with larger, more diverse cohorts and extended follow-up periods is necessary to thoroughly assess and confirm the effectiveness of this probiotic treatment against these severe infectious diseases.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"19 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pueraria thomsonii Benth-Associated Endophytes: A Multifaceted Exploration of Its Bioactive Molecules","authors":"Yu Li, Yu Liu, Yalan Ran, Shiqin Peng, Xiaoman Yuan, Lingyun Zhao, Huiru Sun, Fulian Su, Donghai Huang, Saravana Kumar Pachaiyappan, Meijun He","doi":"10.1007/s12088-024-01368-6","DOIUrl":"https://doi.org/10.1007/s12088-024-01368-6","url":null,"abstract":"<p>Endophytes associated with medicinal plants hold substantial promise as a novel source for therapeutics development. In this study, 11 endophytic fungi and 9 endophytic bacteria were isolated from the roots of <i>Pueraria thomsonii</i>. In the preliminary screening, the ethyl acetate extract of the <i>Apiospora marii</i> FG-Z21 displayed strong inhibitory effects against drug-resistant bacteria <i>Enterococcus faecalis</i> and Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) with MIC values of 1.563 and 0.098 mg/mL, respectively. Furthermore, this extract exhibited significant inhibitory effects on A549 cells with a GI₅₀ value of 0.036 mg/mL and demonstrated a good antioxidant property with an EC₅₀ value of 1.869 mg/mL. Further investigation of the active ethyl acetate extract of <i>A. marii</i> FG-21 led to the isolation of 4-hydroxybenzoic acid (<b>1</b>), 2-acetamidobenzoic acid (<b>2</b>), benzoic acid (<b>3</b>), dibutyl phthalate (<b>4</b>), 3,4-dimethoxybenzoic acid (<b>5</b>), schizostatin (<b>6</b>), ethyl pyroglutamate (<b>7</b>), and anthranilic acid (<b>8</b>). Notably, compounds <b>3</b>, <b>1</b> and <b>2</b> exhibited significant cytotoxicity against A549 cells with GI₅₀ values ranging from 4.97 to 83.08 µg/mL. On the other hand, compounds <b>4</b>, <b>5</b> and <b>6</b> demonstrated potent inhibition of <i>E. faecalis</i> growth with low MIC values ranging from 2 to 4 µg/mL. Our study provides a comprehensive exploration of <i>P. thomsonii</i>-derived endophytes and their compounds highlighting their significant bacteriostatic, antioxidative, and cytotoxic activities that may potentially contribute to drug discovery paradigms.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"5 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesized Ag Nanoparticles as Promising Antibacterial and Antitumor Agents: In Vitro Studies","authors":"Shadi Mansour Hosseini, Atena Soleimani, Sepideh Khaleghi, Shadi Hajrasouliha","doi":"10.1007/s12088-024-01374-8","DOIUrl":"https://doi.org/10.1007/s12088-024-01374-8","url":null,"abstract":"<p>In this work, we used a biocompatible and safe approach for the treatment of health-threatening diseases using the <i>Spinacia oleracea</i> plant. Practically, Ag nanoparticles were green synthesized applying <i>Spinacia oleracea</i> to use against breast cancer cells (MCF-7 and MDA-MB-231 cell lines) and bacteria (<i>S. aureus</i>, <i>S. epidermidis</i>, and <i>P. aeruginosa</i>). Next, analytical techniques (FT-IR, XRD, DLS, TEM, and SEM) were employed for the characterization of nanoparticles. Nanometric size (10 nm to 25 nm in diameter), crystallinity, and spherical and semi-spherical morphology were determined for the biosynthesized Ag nanoparticles. Then, several biomedical tests (MIC, MBC, cell viability (MTT), quantitative gene expression (qRT-PCR), cell cycle arrest, and apoptosis) were performed for investigating the cell suppression capability of Ag nanoparticles and extracted plant precursor. These results indicated MIC of 20 nM, 35 nM, and 35 nM for <i>S. epidermidis</i>, <i>P. aeruginosa</i>, and <i>S. aureus</i> after treatment with Ag nanoparticles, respectively. Cell viabilities of 35% to 90% (for plant precursor) and 55% to 85% (for Ag nanoparticles) were observed for both cancer cells. Expression levels of BRCA1 (3 to 6 folds decrease), BRCA2 (4 to 15 folds decrease), Caspase9 (3.5 folds increase), Bcl2 (3 folds decease), Beclin1 (no considerable shift), and ATG (1.3 folds increase) genes were ascertained in cancer cells after treatment with Ag nanoparticles. Also, the cell cycle arrest (18- and 19-fold increase for MCF-7 and MDA-MB-231, respectively) and apoptosis (22% necrosis for MCF-7 and 17.5% apoptosis for MDA-MB-231) assays have remarkably confirmed the potency of biosynthesized Ag nanoparticles in the treatment of diseases.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"38 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Fluconazole Exposure Promotes Switch Phenotypes With Altered Virulence Traits in the Pathogenic Yeast Candida Tropicalis","authors":"Murilo Moreira dos Santos, Cássia Milena de Souza, Luciana Furlaneto-Maia, Marcia Cristina Furlaneto","doi":"10.1007/s12088-024-01376-6","DOIUrl":"https://doi.org/10.1007/s12088-024-01376-6","url":null,"abstract":"<p><i>Candida tropicalis</i> is an opportunistic yeast pathogen that has the ability to develop resistance to fluconazole. This study evaluated the effect of fluconazole on the rise of phenotypic switching in <i>C. tropicalis</i>, an event that affects virulence in this species<i>. C. tropicalis</i> clinical isolate 49.07 switched reversibly at high frequency (10⁻¹ to 10⁻²) after growth in presence of subinhibitory concentration of fluconazole (0.0625 µg/mL). Phenotypic switching resulted in colonies exhibiting Star and Micro morphologies. A decrease in sensitivity to fluconazole for the Star morphotype was observed. Colonies of both switched morphotypes were associated with higher percentages of filamentous growth relative to the Smooth morphotype (clinical isolate pattern). Under filament-inducing conditions, filamentation was increased for the Star morphotype. The Star and Micro phenotypes exhibited reduced biofilm compared to the Smooth morphotype. The expression of transcription factors (<i>EFG</i>1 and <i>WOR</i>1) was increased in the Micro morphotype compared to the Smooth colonies. Our findings indicate that in vitro exposure of <i>C. tropicalis</i> to fluconazole may promote phenotypic switching and the occurrence of switched morphotypes with altered virulence traits.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"4 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142220313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}