Indian Journal of Microbiology最新文献

{"title":"The Underlying Molecular Mechanisms of Agriculturally Important Microbes: Role in Global Sustainable Development Through Agricultural and Environmental Interventions.","authors":"Basundhara Lenka, Pratikhya Mohanty, Puneet Kumar Singh, Tapan Kumar Adhya, Ranjan K Mohapatra, Snehasish Mishra","doi":"10.1007/s12088-026-01565-5","DOIUrl":"10.1007/s12088-026-01565-5","url":null,"abstract":"<p><p>The present agricultural scenario with soil infertility, low productivity, climate change, environmental pollution and local economic instability challenges calls for coordinated efforts towards global sustainable development. The role of crop-friendly microbes, thus, becomes indispensable. Standard global regulations to devise and implement sustainable cropping and yield improvement measures seem emergent in such context. Microbes that are actively involved in soil physiology and crop growth could increase crop yield and reduce the dependency on agrochemicals, while promoting sustainable agriculture. To advance the sustainable development goals, it was essential to understand molecular mechanisms of microbial activities like N₂-fixation (<i>nif</i> genes), phosphate solubilisation (<i>phoD</i>, <i>appA</i> and <i>phnX</i>), phytohormone production (<i>ipdC</i> for indole-3-acetic acid synthesis), ACC (1-aminocyclopropane-1-carboxylate) deamination (<i>acdS</i>), thermotolerance (<i>hsp</i>) and reactive oxygen species (ROS) detoxification (<i>katE</i> and <i>sodA</i>) that underlie. Formulated crop-associated microbial consortia as synthetic microbial community (SynCom) could extend such benefits. In line with green (sustainable) farming, SynCom approach enhances the stability of a formulated allochthonous microbial community through the synergistic ecological interactions between the participating members and would promote crop productivity with lesser use of agrochemicals. Omics approach, either stand-alone or combined with advanced bioinformatics, is another strategy that employs non-culture techniques to identify microbial genome in an ecological niche and their functional traits. Such insights on the plant-microbe associations and the underlying molecular mechanisms could be useful in enhancing agricultural productivity and crop wellbeing on sustainable bases. This article is an attempt to dissect the recently reported literature, understand the underlying aspects in greater details, and present the current knowledge in a lucid and interlinked manner for a better and insightful understanding of the readers, with the future research directions.</p><p><strong>Graphical abstract: </strong>Plant-microbe interaction supports sustainability in agriculture through biofertilisation and biocontrol activity of PGPR by inducing nitrogen fixation, phosphorus solubilisation, ROS neutralisation, phytostimulation and provides protection from biotic/abiotic stress. This approach helps achieve sustainable development goals, promotes sustainable agriculture, enhances climatic resilience and supports resource efficient farming.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"925-947"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effect of <i>Potentilla nepalensis</i> with Antibiotics: Targeting Bacterial Efflux Pumps Through Pharmacological, and Computational Approaches.","authors":"Subhash Sharma, Vikas Kumar, Chirag N Patel, Anuradha Sourirajan, Kamal Dev","doi":"10.1007/s12088-025-01530-8","DOIUrl":"10.1007/s12088-025-01530-8","url":null,"abstract":"<p><p><i>Potentilla nepalensis</i> (Family: Rosaceae) has been traditionally used to treat burns, gastrointestinal disorders, headaches, dysentery, colds, and skin diseases. This study investigates the synergistic antibacterial interactions between methanolic extracts of <i>P. nepalensis</i> roots and shoots with selected antibiotics, supported by molecular docking and molecular dynamics (MD) simulations to elucidate the underlying mechanisms. Phytochemical profiling revealed comparable constituents in root and shoot extracts, with high-performance liquid chromatography (HPLC) quantification highlighting significant variations in key bioactive compounds. Synergistic antibacterial interactions were evaluated using combinations of roots and shoot extracts with ampicillin and chloramphenicol against <i>S. aureus and E. coli.</i> These combinations demonstrated significant bioactivity enhancements ranging from 5-32.26 folds with ampicillin and 8-62.5 folds with chloramphenicol. Molecular docking revealed strong binding of ellagic acid to the NorA efflux pump (PDB ID: 7LO8) and multidrug transporter protein, AcrB (PDB ID: 4DX5). MD simulations further confirmed the stability of these protein-ligand complexes, supported by MM-GBSA analysis. Pharmacokinetic evaluations indicated poor solubility and low permeability of ellagic acid leading to primary fecal elimination. However, its favorable safety profile and minimal drug-drug interaction suggest its potential as a safe therapeutic agent. The findings underscore the potential of <i>P. nepalensis</i> as an adjuvant in antibiotic therapy, offering a promising strategy to combating drug-resistant bacterial infections. Further in vivo studies and clinical evaluations are warranted to confirm its therapeutic efficacy.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"1033-1048"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic and Metatranscriptomic Insights into the Structure and Function of the Gut Microbial Community of <i>Antheraea assamensis</i> Helfer.","authors":"Dharitri Saikia, Pulakeswar Basumatary, Adhiraj Nath, Jon J Kalita, Kartik Neog, Mihir K Purkait, Utpal Bora","doi":"10.1007/s12088-025-01525-5","DOIUrl":"10.1007/s12088-025-01525-5","url":null,"abstract":"<p><p><i>Antheraea assamensis</i> Helfer is an economically important, endemic, lepidopteran insect native to Northeast India that produces a lustrous golden-coloured silk of distinct quality and durability. To date, the gut microbiota of <i>A. assamensis</i> has remained largely unexplored. The present work aimed to comprehensively identify and characterize the gut microbial community of <i>A. assamensis</i> through culture-independent approach. The gene expression analysis of the gut microbial community was studied through metatranscriptomic analysis. The influence of the host leaf-associated microbiota on larval gut microbial composition and its variation to changes in host plant was also investigated. The results have identified over 30 bacterial and archaeal phyla indicating a highly diverse gut microbial community of <i>A. assamensis</i> dominated by Proteobacteria (25.78%), Patescibacteria (12.77%), Planctomycetota (12.66%), Chloroflexi (8.63%), Acidobacteria (6.85%) and Actinobacteria (3.39%). Functional analysis of <i>A. assamensis</i> gut microbiota through shotgun metagenomic and metatranscriptomic investigation revealed key associations between the insect and its gut microbial community including host leaf digestion, metabolite detoxification, chitinase production and fat body metabolism. The host leaf-associated microbiota was found to occupy a major portion of the total larval gut microbiota. However, the diversity of the larval gut microbiota was greater than the host leaf-associated microbiota. The findings of this study will illustrate the structure of the gut microbial community of <i>A. assamensis</i>, their key interactions with the host organism and the role of host leaf-associated microbiota on the holobiont.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12088-025-01525-5.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"982-1001"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-Friendly Biosynthesis of Silver Nanoparticles using <i>Citrus limon</i> Extract for Mosquito-Repellent and Antimicrobial Fabric Applications.","authors":"U G S Wijayapala, S D Samarasinghe, Chandrika U Gamage","doi":"10.1007/s12088-025-01505-9","DOIUrl":"10.1007/s12088-025-01505-9","url":null,"abstract":"<p><p>Developing eco-friendly mosquito-repellent fabrics using biosynthesized silver nanoparticles (AgNPs) from natural ingredients has attracted significant interest. Mosquito attraction is influenced by carbon dioxide exhalation and body odors caused by bacterial activity on sweat. This study presents a novel approach to synthesizing AgNPs using <i>Citrus limon</i> (lemon leaves) extract through a bio-reduction process with an aqueous solution of silver nitrate (AgNO₃). The nanoparticles were characterized by UV-visible spectroscopy (420-460 nm), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDAX). AgNP-treated fabrics demonstrated 70% retention of mosquito-repellent and antimicrobial properties after 20 wash cycles, outperforming directly treated fabrics. The fabrics also exhibited strong antibacterial activity against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, MRSA, and <i>Pseudomonas aeruginosa</i>. This work highlights the dual functionality of AgNP-treated textiles and offers a sustainable, cost-effective solution for antimicrobial and insect-repellent applications, particularly in public health and healthcare sectors.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"957-965"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Xanthine Oxidase by 1-O-Methyl Chrysophanol, A Hydroxyanthraquinone Isolated from <i>Amycolatopsis Thermoflava</i> ICTA 103.","authors":"Uma Rajeswari Batchu, Bharati Reddi, Joshna Rani Surapaneni, Aruna Jangam, Sunil Misra, Anthony Addlagatta, Prakasham Reddy Shetty","doi":"10.1007/s12088-025-01534-4","DOIUrl":"10.1007/s12088-025-01534-4","url":null,"abstract":"<p><p>The aim of this study was to isolate a potent xanthine oxidase (XO) inhibitor from actinobacteria and to investigate its inhibitory mechanism through in vitro and in vivo models. Culture filtrates from various actinobacterial strains i.e. <i>Amycolatopsis thermoflava</i> ICTA103, <i>Streptomyces luteireticuli</i> ICTA16, <i>Streptomyces kurssanovii</i> ICTA165, and <i>Amycolatopsis lurida</i> ICTA194 were screened for XO inhibitory activity. Among these, <i>A. thermoflava</i> ICTA103 exhibited the strongest inhibition, and 1-O-methyl chrysophanol (OMC) was identified as the lead compound. The cytotoxicity, and XO inhibitory potential of OMC were evaluated using in silico modeling, biochemical assays, in vitro cell lines, and in vivo animal models. Lineweaver-Burk plot analysis determined the IC50 and K_i values of OMC to be 24.8 ± 0.072 μM and 2.218 ± 0.3068 μM, respectively. Molecular docking studies confirmed that OMC effectively binds to the active site of XO. Cell line experiments indicated that OMC is non-toxic and significantly downregulates pro-inflammatory cytokines (IL-8, TNF-α) in a gouty arthritis model. Similarly, in vivo studies in mice showed no toxicity up to a dose of 2000 mg/kg body weight and demonstrated a significant reduction in serum uric acid levels in a hyperuricemic mouse model.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12088-025-01534-4.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"1049-1068"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advancements in Synthetic DNA Based Biosensor Construction Towards Bacterial Diarrhoea Detection: a Review.","authors":"Soumyadeb Bhattacharyya, Shubhajit Bhar, Subhankar Mukherjee, Pradeep Kumar Das Mohapatra, Souvik Pal","doi":"10.1007/s12088-025-01518-4","DOIUrl":"10.1007/s12088-025-01518-4","url":null,"abstract":"<p><p>A major cause of diarrhoea is water because it contains numerous bacteria, viruses, or parasites that irritate the digestive tract. The World Health Organization reported that the death of children below 5 years is a major loss due to a lack of early prediction of the diarrhoea-causing agent. Though conventional microbiological diagnostics are crucial for lab-based prognosis, recent approaches of rapid bacterial diarrhoea detection though biosensor application are widely explored. DNA based biosensors such as Aptamers, DNAzymes, synthetic DNA probes are proven to be excellent bioreceptors towards biosensor development due to their ease of use, improved stability, reusability and cost effectiveness. This article provides a detailed review on the available techniques of bacterial diarrhoea detection using synthetic DNA bioreceptor and signal transduction through optical or electrochemical process. DNA biosensor-based detection of bacteria such as <i>E. coli</i>, <i>Salmonella</i> spp., <i>Shigella</i> spp., <i>Vibrio</i> spp., <i>Klebsiella</i> spp., etc. emerged in last 5 years are covered in detail, in this review. Additionally, a brief discussion is also made on the use of nanomaterials, which provides new possibilities for quick, inexpensive, and selective detection. Moreover, the pros-cons of using synthetic DNA biosensors are also been elaborated in this review. Finally, the future potential of DNA biosensors for disease monitoring and prevention, rationalising medical therapy, and enhancing quality of life are also been discussed. This literature aims to provide comprehensive information on the global status of DNA biosensors and encourage researchers to identify suitable bioreceptors for their experimental pursuits and future discoveries.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"879-896"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural Features of <i>Cryptococcus neoformans</i> Histone Deacetylases Null Mutant Strains.","authors":"Daniel Oliveira da Mata, Lucas da Costa Campos, Martha Viviana Roa-Cordero, Ariane Pandolfo Silveira, Ingrid Gracielle Martins da Silva, Victor Carlos Mello, Luís Alexandre Muehlmann, Bergmann Morais Ribeiro, Sônia Nair Báo, Marcio José Poças-Fonseca","doi":"10.1007/s12088-025-01533-5","DOIUrl":"10.1007/s12088-025-01533-5","url":null,"abstract":"<p><p><i>Cryptococcus neoformans</i> is a pathogen affecting predominantly immunocompromised individuals. Epigenetic regulation is pivotal for controlling <i>C. neoformans</i> virulence traits. Null mutants of histone deacetylase (HDAC) genes demonstrate reduced virulence. Here, we employed scanning (SEM) and transmission electron microscopy (TEM) to examine the ultrastructure of <i>C. neoformans HDA1</i> and <i>HOS2</i> null mutant strains<i>.</i> SEM revealed unipolar budding in the H99, <i>hda1</i>∆ and <i>hos2</i>∆ strains, whereas the <i>hda1∆hos2</i>∆ double mutant displayed evident multipolar budding. The <i>hos2</i>∆ strain exhibited defective capsule. TEM analysis showed a thicker cell wall for <i>hda1</i>∆ in comparison to H99, while the <i>hos2</i>∆ and <i>hda1∆hos2</i>∆ mutants displayed thinner cell walls. The <i>hos2</i>∆ and <i>hda1∆/hos2</i>∆ strains exhibited mitochondrial swelling. The <i>hda1∆hos2</i>∆ mutant also presented morphological features suggestive of cell degeneration. These findings highlight the critical role of HDACs in <i>C. neoformans</i> morphogenesis, which is essential to host cell-yeast interaction.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12088-025-01533-5.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"1098-1102"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Engineering in Microbial System for Riboflavin Production: A Comprehensive Review.","authors":"Ananya Rana, Neetu Kumra Taneja, Namita Singh, Anil Kumar Puniya","doi":"10.1007/s12088-025-01531-7","DOIUrl":"10.1007/s12088-025-01531-7","url":null,"abstract":"<p><p>Vitamin B2 (riboflavin), a precursor of the essential coenzymes flavin mononucleotide and flavin adenine dinucleotide, plays a critical role in numerous cellular metabolic pathways, including redox reactions, energy production, and biosynthetic processes. Due to its fundamental importance in human and animal nutrition, riboflavin has found wide application across the food, pharmaceutical, nutraceutical, and cosmetic industries. In recent decades, the development of metabolic engineering techniques has revolutionized riboflavin production, transitioning from traditional chemical synthesis to environmentally sustainable microbial fermentation. Microorganisms such as <i>A. gossypii</i>, <i>Candida famata</i>, and <i>Bacillus subtilis</i> have been extensively engineered to enhance riboflavin yield at an industrial scale. Key metabolic engineering strategies include reprogramming the purine metabolic network, mutating ribulose-5-phosphate 3-epimerase, and relieving regulatory constraints on purine biosynthesis. These advances have significantly improved the efficiency, cost-effectiveness, and sustainability of industrial riboflavin production. This review article presents an in-depth analysis of biotechnological methods aimed at increasing riboflavin production, ensuring a consistent supply of this crucial micronutrient for both human and animal nutrition. It stands as a significant reference for researchers and industry stakeholders.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"897-911"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and Characterisation of Low-Cost Amylase from Fermented Cassava Mash Using <i>Saccharomyces cerevisiae</i> and <i>Lactobacillus</i> sp. for Industrial Applications.","authors":"David M Sanni, Emmanuel Awojulu, Olusola T Lawal, Ganiyu Oboh, Segun V Omotoyinbo, Olufunke F Ajeigbe, Adeniyi A Adebayo, Oladipo O Olaniyi, Damilola Fasan, Olugbenga O Awolu, Aderonke I Olagunju","doi":"10.1007/s12088-025-01527-3","DOIUrl":"10.1007/s12088-025-01527-3","url":null,"abstract":"<p><p>Cassava mash wastewater is a largely underutilised agro-industrial byproduct with potential as a sustainable substrate for enzyme production and waste valorisation. This study investigated the biochemical properties and potential applications of amylase produced via solid-state fermentation of cassava root wastewater using <i>Saccharomyces cerevisiae</i> and <i>Lactobacillus</i> sp. (SLL). The crude enzyme was extracted and purified through ammonium sulfate precipitation, ion-exchange, and gel-filtration chromatography. Purification increased enzyme purity 12.9-fold with a recovery yield of 17%. The partially purified amylase exhibited broad catalytic activity across a wide pH and temperature spectrum, with an optimum at 30 °C and pH 2. It retained 90-70% activity between pH 2-12 and 86-76% activity at 30-90 °C after 3 h, demonstrating exceptional stability. Activity was largely unaffected by Na⁺, K⁺, Ca²⁺, Mg²⁺, Cu²⁺, and Zn²⁺, but inhibited by Hg²⁺ at 10 mM. Strong inhibition was observed with SDS (10%) and mercaptoethanol (5-10%), while no significant inhibition was recorded with L-cysteine, sodium azide, Tween-20, or urea. Remarkably, polar organic solvents enhanced enzyme activity. Furthermore, the amylase displayed high compatibility with commercial detergents and effective stain removal, underlining its industrial relevance. Collectively, these findings highlight the enzyme's robustness, solvent tolerance, and detergent compatibility, positioning it as a promising candidate for detergent formulations and broader biotechnological applications.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12088-025-01527-3.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"1002-1016"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward Actionable and Equitable AMR Forecasting: A Standards-First Blueprint Linking Clinical and Environmental Resistomes.","authors":"M Vijayasimha, S Sreedevi","doi":"10.1007/s12088-025-01537-1","DOIUrl":"10.1007/s12088-025-01537-1","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) forecasting-here defined as the use of statistical or machine-learning models to anticipate future resistance trajectories, patterns, and emerging hotspots-has progressed rapidly but remains fragmented by heterogeneous standards, limited cross-sector integration, and insufficient ethical and equity safeguards. Building upon recent contributions to the <i>Indian Journal of Microbiology</i>, we present a standards-first blueprint structured around four pillars: (i) a minimal common data element (mCDE) backbone for harmonized AMR data architecture; (ii) systematic integration of environmental and food-chain resistomes to support One Health prediction models; (iii) a five-level Forecast-to-Action Readiness Level (FRL) framework to link forecasts with real-world stewardship and public-health interventions; and (iv) explicit ethics, transparency, equity, and capacity-building requirements to ensure fair participation of low- and middle-income countries (LMICs). The blueprint emphasizes reproducibility, environmental complementarity, and decision-aware forecasting, with alignment to WHO GLASS and regional AMR programs. This structured protocol offers a scalable and implementation-ready pathway toward equitable, cross-sector AMR forecasting networks.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"66 4","pages":"1103-1105"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13447615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}