Whole Genome Sequencing of Carbapenem-Resistant Enterobacter hormaechei Isolated from Thiruvananthapuram, Kerala (India)

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Indian Journal of Microbiology Pub Date : 2024-08-22 DOI:10.1007/s12088-024-01377-5

Parvathi Vaikkathillam, Praveen Kumar, S. Manjusree, Minsa Mini, Devi Jayakumar, Amjesh Revikumar

{"title":"Whole Genome Sequencing of Carbapenem-Resistant Enterobacter hormaechei Isolated from Thiruvananthapuram, Kerala (India)","authors":"Parvathi Vaikkathillam, Praveen Kumar, S. Manjusree, Minsa Mini, Devi Jayakumar, Amjesh Revikumar","doi":"10.1007/s12088-024-01377-5","DOIUrl":null,"url":null,"abstract":"The rapid emergence of carbapenem-resistant Enterobacteriaceae is a critical public health issue across the globe. Widespread antibiotic resistance to multiple drugs has been reported in clinical isolates of Enterobacter hormaechei. In the present investigation, we report the whole genome sequencing (WGS) of a multi-drug resistant (MDR), clinical strain of E. hormaechei, GC26 isolated from Thiruvananthapuram, Kerala (India). Using the Oxford Nanopore sequencing platform, we identified key drug-resistance genes (bla, tet, armA, qnrB, and fos) and virulence genes (fimD, csgD, csgE, csgF, and csgG) in GC26. Multi-Locus Sequence Typing (MLST) analysis of GC26 revealed a novel sequence type (ST*ba28). Phylogenetic analysis showed that the GC26 genome occupies a distinct branch within the Enterobacter genus, closely related to Indian strains of E. hormaechei rather than the global population. This study provides valuable insights into the genetic basis of multidrug resistance in E. hormaechei. These findings underscore the critical need for continuous monitoring and robust epidemiological surveillance of MDR E. hormaechei strains.","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"61 1","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12088-024-01377-5","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The rapid emergence of carbapenem-resistant Enterobacteriaceae is a critical public health issue across the globe. Widespread antibiotic resistance to multiple drugs has been reported in clinical isolates of Enterobacter hormaechei. In the present investigation, we report the whole genome sequencing (WGS) of a multi-drug resistant (MDR), clinical strain of E. hormaechei, GC26 isolated from Thiruvananthapuram, Kerala (India). Using the Oxford Nanopore sequencing platform, we identified key drug-resistance genes (bla, tet, armA, qnrB, and fos) and virulence genes (fimD, csgD, csgE, csgF, and csgG) in GC26. Multi-Locus Sequence Typing (MLST) analysis of GC26 revealed a novel sequence type (ST*ba28). Phylogenetic analysis showed that the GC26 genome occupies a distinct branch within the Enterobacter genus, closely related to Indian strains of E. hormaechei rather than the global population. This study provides valuable insights into the genetic basis of multidrug resistance in E. hormaechei. These findings underscore the critical need for continuous monitoring and robust epidemiological surveillance of MDR E. hormaechei strains.

Abstract Image

查看原文本刊更多论文

从印度喀拉拉邦 Thiruvananthapuram 分离的耐碳青霉烯类肠杆菌的全基因组测序

耐碳青霉烯类肠杆菌科细菌的迅速出现是全球范围内一个严重的公共卫生问题。据报道，在霍乱弧菌的临床分离株中广泛存在对多种药物的抗生素耐药性。在本研究中，我们报告了从喀拉拉邦 Thiruvananthapuram（印度）分离出的一株具有多重耐药性（MDR）的霍拉氏肠杆菌临床菌株 GC26 的全基因组测序（WGS）结果。利用牛津纳米孔测序平台，我们确定了 GC26 中的关键耐药基因（bla、tet、armA、qnrB 和 fos）和毒力基因（fimD、csgD、csgE、csgF 和 csgG）。对 GC26 的多焦点序列分型（MLST）分析发现了一种新的序列类型（ST*ba28）。系统进化分析表明，GC26 基因组在肠杆菌属中属于一个独特的分支，与印度的荷马菌株而非全球的荷马菌株关系密切。这项研究为了解霍拉氏肠杆菌耐多药的遗传基础提供了宝贵的见解。这些发现强调了对荷马菌耐多药菌株进行持续监测和强有力的流行病学监控的迫切需要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Indian Journal of Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY

CiteScore

6.00

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： Indian Journal of Microbiology is the official organ of the Association of Microbiologists of India (AMI). It publishes full-length papers, short communication reviews and mini reviews on all aspects of microbiological research, published quarterly (March, June, September and December). Areas of special interest include agricultural, food, environmental, industrial, medical, pharmaceutical, veterinary and molecular microbiology.