Indian Journal of Medical Microbiology最新文献

{"title":"An in-silico study exploring the differences between the G protein of Chandipura virus isolates with respect to their antigenic sites and their interaction with vimentin","authors":"Deepak Jena ,&nbsp;Prabhudutta Mamidi ,&nbsp;Satya Ranjan Singh ,&nbsp;Manisha Tripathy ,&nbsp;Amrita Ray ,&nbsp;Diksha Mohapatra ,&nbsp;Sailendra Panda ,&nbsp;Sunil Kumar Raghav ,&nbsp;Bhagirathi Dwibedi ,&nbsp;Soma Chattopadhyay ,&nbsp;Baijayantimala Mishra","doi":"10.1016/j.ijmmb.2026.101083","DOIUrl":"10.1016/j.ijmmb.2026.101083","url":null,"abstract":"<div><h3>Purpose</h3><div>Chandipura virus (CHPV), belonging to the <em>Rhabdoviridae</em> family, is an emerging pathogen linked to encephalitis outbreaks, especially in children under 15 years of age. CHPV is primarily spread by sandflies and has led to several outbreaks in India, but CHPV strains from African nations namely Senegal, Kenya and Nigeria are not associated with human infections. This study involved an <em>in-silico</em> analysis aimed at exploring the differences in the G protein between the Indian and African CHPV isolates which would explain the above anomaly.</div></div><div><h3>Methods</h3><div>Multiple Sequence Alignment was carried out to identify the amino acid substitution sites between all the Indian and the African CHPV isolates. Additionally, Profile Sequence Alignment (PSA) analysis was also conducted to localize the identified conserved amino acid substitutions between the Indian and the African CHPV G protein, focusing on potential antigenic sites and critical viral domains. Furthermore, an <em>in-silico</em> analysis has been carried out to assess the binding affinity of the CHPV G protein of different countries with the host co-receptor vimentin that is known to be involved in virus entry.</div></div><div><h3>Results</h3><div>Our results indicated that the Indian and the African CHPV G protein sequences exhibited amino acid variations at 19 conserved sites, which were localized within the antigenic sites and other critical viral domains, including the fusogenic site of the CHPV G protein. Furthermore, our analysis predicted significant alterations in either solvent accessibility or torsional angle rotation stemming from the amino acid variations at the aforementioned sites. Additional in-silico analysis also revealed that the Indian CHPV G protein exhibited a markedly superior binding affinity with vimentin compared to its African counterparts.</div></div><div><h3>Conclusion</h3><div>Therefore, the current study provides important findings regarding the variations of the CHPV G protein and highlighting essential amino acid residues that may be targeted for development of future antiviral therapies.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"60 ","pages":"Article 101083"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breakthrough invasive fungal infections during posaconazole prophylaxis in patients with hematologic malignancies at a tertiary care center - a 5-year audit from south India","authors":"P.K. Sowndharya ,&nbsp;Anjima Madhu ,&nbsp;B. Gayathri Devi ,&nbsp;Farhana Basheer ,&nbsp;Selin C. Joy ,&nbsp;Mazhuvancherry Kesavan Unnikrishnan ,&nbsp;Rema Ganapathi","doi":"10.1016/j.ijmmb.2026.101079","DOIUrl":"10.1016/j.ijmmb.2026.101079","url":null,"abstract":"<div><h3>Purpose</h3><div>Breakthrough invasive fungal infections (BtIFIs) are an emerging concern in patients with hematological malignancies (HM), despite the use of primary antifungal prophylaxis. Posaconazole (PCZ), a broad-spectrum triazole antifungal, is FDA-approved for primary prophylaxis in high-risk settings. However, data on the real-world burden and characteristics of BtIFIs from low- and middle-income countries (LMICs) are limited. This study aimed to evaluate the incidence, predictors, and microbiological and radiological features of BtIFIs in HM patients receiving PCZ prophylaxis at a tertiary care centre in India.</div></div><div><h3>Methods</h3><div>Following, Institutional Ethics Committee approval, we enrolled 176 patients on PCZ prophylaxis (adults: 600 mg on day 1, then 300 mg/day; pediatrics: 14 mg/kg on day 1, then 7 mg/kg/day) for IFI prevention during treatment for HM (n = 176) at a tertiary care hospital. We retrospectively reviewed medical records from 2019 to 2023 and prospectively collected data in 2024. Independent sample <em>t</em>-test and a chi-square test were used for statistical analysis.</div></div><div><h3>Results</h3><div>Of 176 patients, 59 (33.5%) developed BtIFIs [culture-proven BtIFI = 20 (33.8%); probable BtIFI = 39 (66.1%)], with an incidence of 336/1000 patients. Aspergillus (46.66%) and Candida (28.88%) spp. were the most commonly isolated pathogens. Severe neutropenia (lasting 5-15 days) was the most significant risk factor for BtIFI (54%, p &lt; 0.001). Comorbidities, Proton Pump Inhibitors (PPIs) and the chemotherapy regimen were not significant risk factors.</div></div><div><h3>Conclusion</h3><div>The High incidence of BtIFI observed may be attributed to the unavailability of therapeutic dose monitoring (TDM), variations in pathogenic strains, and environmental factors conducive to fungal infections (warmth and high humidity), implying that strengthened surveillance and prophylaxis strategies are essential.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"60 ","pages":"Article 101079"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146219398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of native Lactobacillus cell-free supernatant against diarrheagenic Escherichia coli isolated from hospitalized patients in northern Iran","authors":"Farzaneh Rafie Sedaghat ,&nbsp;Ali Mojtahedi ,&nbsp;Meysam Hasannejad-Bibalan ,&nbsp;Tofigh Yaghubi Kalurazi ,&nbsp;Tohid Samadpour Zahmat-dar","doi":"10.1016/j.ijmmb.2026.101063","DOIUrl":"10.1016/j.ijmmb.2026.101063","url":null,"abstract":"<div><h3>Background and aims</h3><div>Antibiotic resistance is a growing concern in the fight against <em>Escherichia coli</em>, a pathogen that causes various intestinal diseases. The present study explores the antibacterial effect of <em>lactobacillus</em> on <em>E. coli</em> pathotypes.</div></div><div><h3>Methods</h3><div>Inpatients stool samples were collected and <em>E. coli</em> pathotypes were identified. The antibacterial effect of five <em>Lactobacillus</em> on the pathotypes was investigated by broth microdilution and well diffusion methods.</div></div><div><h3>Results</h3><div>The CFS of <em>L. plantarum, L. rueteri</em> and <em>L. acidophilus</em> had the greatest antibacterial effect.</div></div><div><h3>Conclusion</h3><div>Postbiotics have shown significant bactericidal effects against <em>E. coli</em> pathotypes. Their bactericidal effect can prevent the colonization of these pathotypes in hospitalized patients.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"60 ","pages":"Article 101063"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146029397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the microbiological experience in exploring spinal TB cases from a tertiary care center","authors":"Jaya Biswas ,&nbsp;Kiran Bala ,&nbsp;Neha Nityadarshini ,&nbsp;Leander Jose ,&nbsp;Vivek Shankar ,&nbsp;Urvashi B. Singh","doi":"10.1016/j.ijmmb.2026.101050","DOIUrl":"10.1016/j.ijmmb.2026.101050","url":null,"abstract":"<div><h3>Purpose</h3><div>Spinal tuberculosis, a severe form of extrapulmonary tuberculosis, poses significant diagnostic and therapeutic challenges, often leading to neurological complications and deformity. Microbiological confirmation is crucial for diagnosis and effective treatment. We aim to evaluate the microbiological profile and diagnostic yield of various methods in spinal TB cases at a tertiary care centre and to correlate with clinical features and treatment outcomes.</div></div><div><h3>Methods</h3><div>A prospective study was conducted on samples from patients with suspected spinal TB (n = 58) from July 2023 to September 2024. All samples were processed using Ziehl-Neelsen (ZN) staining for acid-fast bacilli (AFB), culture on Lowenstein-Jensen (LJ) medium, BACTEC MGIT 960 liquid culture system, and GeneXpert MTB/RIF assay for rapid detection of MTB and rifampicin sensitivity. Relevant demographic, clinical, and radiological data were collected and analyzed. Based on susceptibility to drugs of culture isolates, treatment was started to see the outcome, and follow-ups were done with the patients.</div></div><div><h3>Results</h3><div>A total of 19 spinal TB cases were microbiologically confirmed, out of 58 clinically suspected spinal TB cases, with a male predominance (57.9 %) and age range of 13–72 years. The most common symptom was lower back pain (89.5 %). GeneXpert was positive in all cases, detecting rifampicin resistance in 7(36.8 %). Culture was positive in 11 cases. ZN staining was positive in 15.8 % of direct samples. Histopathology showed granulomatous inflammation in 9 (47.3 %) of cases. MRI confirmed infective spinal involvement in 17(89.5 %) patients. MDR-TB regimen was initiated in 7 patients. Overall recovery was good, except one case of neuropathy and one mortality.</div></div><div><h3>Conclusions</h3><div>A combination of smear microscopy, culture, and molecular diagnostics significantly improves the microbiological diagnosis of spinal TB. GeneXpert offers rapid, reliable results, especially in rifampicin resistance detection. Early and accurate microbiological confirmation, coupled with clinical-radiological correlation, is essential for effective management and improved patient outcomes.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"60 ","pages":"Article 101050"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of practical in-vitro methods for testing synergy between ceftazidime-avibactam and aztreonam against metallo-β-lactamase and serine carbapenemase-producing Enterobacterales species","authors":"Neha Sunil Bawankar ,&nbsp;Samrin Ejaz Hussain Sayed ,&nbsp;Swati M. Bhise ,&nbsp;Rajni Gaind","doi":"10.1016/j.ijmmb.2026.101073","DOIUrl":"10.1016/j.ijmmb.2026.101073","url":null,"abstract":"<div><h3>Background</h3><div>The emergence of multidrug-resistant (MDR) strains, including those producing metallo-β-lactamases (MBL), necessitates the development of effective therapeutic strategies. The combination of ceftazidime-avibactam (CZA) and aztreonam (ATM) has shown promise as a potential treatment option. This study aimed to evaluate practical and accessible antimicrobial susceptibility testing (AST) methods for assessing the synergy of CZA and ATM in carbapenemase-producing <em>Enterobacterales</em> species, particularly in resource-limited settings with high patient volumes.</div></div><div><h3>Methods</h3><div>This cross-sectional study was conducted at a tertiary care hospital in Central India from October 2023 to July 2024. It compared different synergy testing methods to the gold-standard broth microdilution (BMD) method in genotypically confirmed carbapenemase-producing isolates. The study evaluated various synergy methods, such as the E-strip, disk replacement, inverse D-zone, and disk approximation.</div></div><div><h3>Results</h3><div>Of the 60 <em>Enterobacterales</em> isolates, most carried the <em>bla</em>_NDM gene (88.33%, 53/60). Using the BMD method, 73.33% (44/60) of the isolates showed synergy between CZA and ATM. The E-strip method was found to have almost perfect agreement (κ = 1) with BMD, making it highly reliable. However, it may be impractical for high-volume laboratories due to cost and complexity. On the other hand, the disk replacement and inverse D-zone methods demonstrated high sensitivity and specificity, with perfect (κ = 0.874) and substantial (κ = 0.803) agreement with BMD, respectively, providing more practical and cost-effective alternatives. However, the disk approximation method showed lower sensitivity and moderate agreement with BMD (κ = 0.510), making it less reliable.</div></div><div><h3>Conclusion</h3><div>The combination of CZA and ATM demonstrated significant synergistic results in MBL and serine carbapenemase-producing <em>Enterobacterales</em> species. The disk replacement and inverse D-zone methods are feasible and cost-effective for CZA and ATM synergy testing in routine AST within resource-limited settings, guiding treatment decisions.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"60 ","pages":"Article 101073"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an enhanced LAMP-based method for early identification of Streptococcus pneumoniae through LytA gene amplification","authors":"Jignisha S. Patel ,&nbsp;Jigna P. Naik ,&nbsp;Dhruv N. Desai","doi":"10.1016/j.ijmmb.2026.101047","DOIUrl":"10.1016/j.ijmmb.2026.101047","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Streptococcus pneumoniae</em> is a leading cause of invasive bacterial disease in developed and developing countries. The <em>Lyt</em>A gene acts as a key pneumococcal virulence factor. No simple, cost-effective technology exists to detect this gene. In this study, we developed and validated a quick and visible LAMP assay targeting the <em>Lyt</em>A gene for pneumococci detection.</div></div><div><h3>Methodology</h3><div>Visual LAMP assay was performed to amplify the <em>S. pneumoniae Lyt</em>A gene using five primers. <em>S. pneumoniae</em> ATCC® 49619™ was employed as a positive control, and 19 pathogenic bacterial strains without the <em>Lyt</em>A gene and 20 clinical isolates with the gene were utilized to test LAMP assay specificity. To assess sensitivity, the visual LAMP assay was compared to PCR using ten-fold serial dilutions of Pneumococcal ATCC DNA. The clinical sensitivity and specificity of the enhanced LAMP assay were tested with 25 pneumonia-suspected clinical samples. In addition, standard microbial culture and PCR analysis were also performed for comparison purposes.</div></div><div><h3>Results</h3><div>After 60 min of incubation at 65 °C, the <em>S. pneumoniae</em> ATCC strain and all 20 clinical pneumococci with defined primers amplified <em>Lyt</em>A target DNA and gave a color change from pink to yellow. DNA amplification was absent in 19 pathogenic bacterial strains without <em>Lyt</em>A genes under the same circumstances. The detection limit for the improved LAMP assay was 6 CFU/mL (0.425 fg/μL). Given conventional microbiological culture as the gold standard, the PCR assay provided two false positives, whereas the LAMP assay gave no false positives with 25 clinical samples. The LAMP assay exhibited 100 % sensitivity, specificity, PPV and NPV (95 % CI) utilizing the conventional culture method as a gold standard.</div></div><div><h3>Conclusion</h3><div>The enhanced LAMP assay visually detects the pneumococcal <em>Lyt</em>A gene faster, simpler, more sensitively, and more specifically than PCR and microbiological methods. This enhanced LAMP method is suited for low-resource population screening and diagnosis.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"60 ","pages":"Article 101047"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145959240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic accuracy of urinary lipoarabinomannan antigen detection for the diagnosis of pulmonary and extra pulmonary tuberculosis (EPTB) in HIV negative population","authors":"Kiran Bala ,&nbsp;Rekha Rathee ,&nbsp;Prayas Sethi ,&nbsp;Praveen Bharti ,&nbsp;Bhavuk Garg ,&nbsp;Anant Mohan ,&nbsp;Urvashi B. Singh","doi":"10.1016/j.ijmmb.2026.101053","DOIUrl":"10.1016/j.ijmmb.2026.101053","url":null,"abstract":"<div><h3>Background</h3><div>Urine antigen testing seems promising tool to detect tuberculosis but data is scarce for pulmonary and extrapulmonary tuberculosis in HIV negative population.</div></div><div><h3>Aims</h3><div>This prospective cross-sectional study was designed to assess the urinary lipoarabinomannan antigen (LAM) diagnostic accuracy of pulmonary and extrapulmonary tuberculosis in HIV negative population.</div></div><div><h3>Materials &amp; methods</h3><div>Suspected pulmonary (PTB) and extrapulmonary tuberculosis (EPTB) patients were enrolled and samples were subjected to routine diagnostic modalities. Urine samples were subjected to LAM test using lateral flow assay of Abbott TB LAM &amp; results were statistically evaluated to gold standard culture, microbiological reference standards (MRS).</div></div><div><h3>Results</h3><div>Total 224 patients of suspected tuberculosis were enrolled in the study (23.21 % PTB and 76.79 % EPTB). Microbiologically confirmed tuberculosis was detected in 44 (19.6 %), PTB (48.0 %) &amp; EPTB (11.0 %) cases. <em>Mycobacterium tuberculosis</em> was detected in ZN stain (16.5 %), MGIT liquid culture (14.7 %), Gene-Xpert MTB/Rif (17.4 %), urinary LAM (22.7 %). Sensitivity, specificity, PPV and NPV of urine LAM against the gold standard test (MGIT) were 60.61 %, 83.77 %, 39.22 %, 92.49 %; and as per the MRS criteria these values were 48.0 %, 84.5 %, 47.1 %, 85.0 % respectively. Among smear positive samples sensitivity, specificity, PPV, NPV of LAM were 65.5 %, 50.0 %, 82.6 %, 28.6 % respectively.</div></div><div><h3>Conclusion</h3><div>Urine LAM performed better than previously reported in HIV-negative populations but remains suboptimal as a point-of-care test. This study showed higher sensitivity and PPV for pulmonary TB in MRS smear-positive cases, better specificity and NPV for extrapulmonary TB, good performance in smear-negative extrapulmonary TB, and promising utility in unconfirmed TB.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"60 ","pages":"Article 101053"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the drug prescribing pattern in a tertiary care trauma centre outpatient department in North India: A cross-sectional study","authors":"Madhumitha Balasubramanian ,&nbsp;Projoyita Samanta ,&nbsp;Parul Singh ,&nbsp;Sushma Sagar ,&nbsp;Subodh Kumar ,&nbsp;Deepak Gupta ,&nbsp;Kamran Farooque ,&nbsp;Vijay Sharma ,&nbsp;Vivek Trikha ,&nbsp;Purva Mathur","doi":"10.1016/j.ijmmb.2026.101080","DOIUrl":"10.1016/j.ijmmb.2026.101080","url":null,"abstract":"<div><h3>Purpose</h3><div>There is a need for rational prescribing of drugs to achieve healthcare outcomes. One of the major consequences of irrational drug use is the emergence of antibiotic resistance. Therefore, drug prescribing practices must be scrutinized at regular intervals to ensure patient safety. In this study, we aimed to evaluate the prescribing pattern of physicians using the ‘WHO-recommended core prescribing indicators’ in the OPD of a tertiary care trauma centre in North India.</div></div><div><h3>Materials and methods</h3><div>This was a prospective, cross-sectional study conducted at all the outpatient departments of a tertiary care trauma centre hospital for a period of 3 months from February to May 2024. A total of 822 prescriptions were assessed based on the World Health Organization (WHO) core prescribing indicators according to guidelines and methods for data consistency. Descriptive statistical analyses (frequencies, mean, percentages, and standard deviation) were used to report the data.</div></div><div><h3>Results</h3><div>The WHO core prescribing indicators analysis showed that the average number of drugs per encounter was 1.25, 47.47% of the drugs were prescribed by generic name, and duration was mentioned only for 55.04% drugs. The percentage of antibiotics prescribed per encounter was 4.86% which was much lower than the ideal WHO recommendations. The most frequently prescribed antibiotic was Cefuroxime, which belongs to WATCH group according to AWaRe classification list, and only 53.68% (n = 553) of the drugs were from the National List of Essential Medicines.</div></div><div><h3>Conclusion</h3><div>The present study on audit of prescription practices was performed using ‘WHO core prescribing indicators, highlights adherence to rationality and completeness. Improvement is needed in generic prescribing and completeness of prescriptions. Regular audits and AWaRe-based practices could enhance rational, cost-effective healthcare delivery.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"60 ","pages":"Article 101080"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146219429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent MALDI-TOF MS identification failure for Sphingomonas paucimobilis from blood cultures: A single-center observation","authors":"Shikhir Malhotra,&nbsp;Vibhor Tak","doi":"10.1016/j.ijmmb.2025.101009","DOIUrl":"10.1016/j.ijmmb.2025.101009","url":null,"abstract":"","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"59 ","pages":"Article 101009"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145512646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of the diagnostic value of polymerase chain reaction-based nucleic acid detection methods for pertussis","authors":"Fengyuan He ,&nbsp;Limin Mei ,&nbsp;Linzi Zeng","doi":"10.1016/j.ijmmb.2025.101031","DOIUrl":"10.1016/j.ijmmb.2025.101031","url":null,"abstract":"<div><h3>Introduction</h3><div>Pertussis is an acute respiratory disease primarily caused by <em>Bordetella pertussis</em>, and its incidence has shown a resurgence. Polymerase chain reaction (PCR) is a recognized molecular detection method, and various related detection methods have emerged.</div></div><div><h3>Methods</h3><div>This study is performed according to the PRISMA checklist. Relevant literature is adopted from the online databases. The quality of the included studies is assessed based on the REVMAN software (version 5.4). Statistical analysis is performed by STATA 14.0 and Meta-DiSc 1.4 software.</div></div><div><h3>Results</h3><div>The results of this meta-analysis show that four different PCR techniques—conventional PCR, real-time quantitative PCR (Q-PCR), multiplex PCR, and nested PCR—have a pooled sensitivity of 0.95 (95 % CI: 0.93–0.97), pooled specificity of 0.93 (95 % CI: 0.89–0.95), pooled positive likelihood ratio (PLR) of 12.22 (95 % CI: 8.62–17.33), pooled negative likelihood ratio (NLR) of 0.05 (95 % CI: 0.03–0.08), and pooled diagnostic odds ratio (DOR) of 244.88 (95 % CI: 133.34–449.72). Multiplex PCR has the highest pooled specificity, PLR, and DOR. Nested PCR has the highest sensitivity and the lowest NLR. PCR testing holds significant value in the early diagnosis of pertussis. Multiplex PCR, nested PCR, and Q-PCR outperform conventional PCR in diagnostic performance.</div></div><div><h3>Conclusions</h3><div>Each type of PCR test has its advantages and disadvantages, and clinical selection should consider factors such as testing purpose, personnel, and conditions.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"59 ","pages":"Article 101031"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}