Human Cell最新文献_第3页

Long noncoding RNA small nucleolar RNA host gene 5 facilitates neuropathic pain in spinal nerve injury by promoting SCN9A expression via CDK9 长非编码 RNA 小核 RNA 宿主基因 5 通过 CDK9 促进 SCN9A 的表达，从而促进脊神经损伤中的神经病理性疼痛

IF 4.3 3区生物学

Human Cell Pub Date : 2024-01-02 DOI: 10.1007/s13577-023-01019-w

Changsheng Wang, Rongsheng Chen, Xitian Zhu, Xiaobo Zhang, Nancheng Lian

{"title":"Long noncoding RNA small nucleolar RNA host gene 5 facilitates neuropathic pain in spinal nerve injury by promoting SCN9A expression via CDK9","authors":"Changsheng Wang, Rongsheng Chen, Xitian Zhu, Xiaobo Zhang, Nancheng Lian","doi":"10.1007/s13577-023-01019-w","DOIUrl":"https://doi.org/10.1007/s13577-023-01019-w","url":null,"abstract":"This study aims to explore the functions and mechanisms of long noncoding RNA small nucleolar RNA host gene 5 (SNHG5) in chronic constriction injury (CCI)-induced neuropathic pain (NP). An NP rat model was established using the CCI method and the NP severity was evaluated by paw withdrawal threshold (PWT) and paw withdrawal latency (PWL). The expression of SNHG5, CDK9, and SCN9A was quantified in rat dorsal root ganglion, in addition to the detections of apoptosis, pathological changes, neuron number, and the co-localization of Nav1.7 and cleaved caspase-3 with NeuN. In ND7/23 cells, the apoptosis and lactate dehydrogenase concentration were assessed, as well as the relationship between SNHG5, CDK9, and SCN9A. In the dorsal root ganglion of CCI-treated rats, SNHG5 and SCN9A were upregulated and downregulation of SNHG5 suppressed SCN9A expression, increased the PWT and PWL, blocked neuroinflammation and neuronal apoptosis, and alleviated NP. Mechanistically, SNHG5 recruited CDK9 to enhance SCN9A-encoded Nav1.7 expression and promoted peripheral neuronal apoptosis and injury. In addition, SCN9A overexpression nullified the alleviative effects of SNHG5 deficiency on NP and neuron loss in CCI rats. In conclusion, SNHG5 promotes SCN9A-encoded Nav1.7 expression by recruiting CDK9, thereby facilitating neuron loss and NP after spinal nerve injury, which may offer a promising target for the management of NP.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139083540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research hotspots and emerging trends in mesenchymal stem/stromal cells in bronchopulmonary dysplasia 支气管肺发育不良间充质干细胞/基质细胞的研究热点和新趋势

IF 4.3 3区生物学

Human Cell Pub Date : 2023-12-30 DOI: 10.1007/s13577-023-01018-x

Meng Ao, Heqian Ma, Meizhen Guo, Xuelin Dai, Xiaoying Zhang

{"title":"Research hotspots and emerging trends in mesenchymal stem/stromal cells in bronchopulmonary dysplasia","authors":"Meng Ao, Heqian Ma, Meizhen Guo, Xuelin Dai, Xiaoying Zhang","doi":"10.1007/s13577-023-01018-x","DOIUrl":"https://doi.org/10.1007/s13577-023-01018-x","url":null,"abstract":"Bronchopulmonary dysplasia (BPD) is a prevalent lung disease in neonates that is associated with numerous complications and high mortality. The promising approach to treat BPD is the use of mesenchymal stem cells (MSCs), However, the current treatment of MSCs presents safety concerns, including occlusion of blood vessels and tumorigenicity. In this study, relevant publications from the Web of Science Core Collection were downloaded in January 2023. The acquired data were analyzed and predicted for trends and hotspots in this field using CiteSpace software. Results revealed that in recent years, the focus of co-cited references has been primarily on the clinical studies of MSCs and the application of MSCs derivatives for treating BPD models. The keywords that have gained attention are extracellular vesicles and exosomes. The United States has emerged as the most influential co-authoring country in this field. Among the co-cited journals, the American Journal of Respiratory and Critical Care Medicine holds the highest influence. Thus, this study provides trends in publications, collaboration, research interests, and hotspots, and provides clues for novel ideas and strategies in to further MSCs treatments for BPD.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":"121 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139070471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of iPSC lines (KAIMRCi003A, KAIMRCi003B) from a Saudi patient with Dravet syndrome carrying homozygous mutation in the CPLX1 gene and heterozygous mutation in SCN9A 从一名携带 CPLX1 基因同源突变和 SCN9A 基因杂合突变的沙特籍德雷维特综合征患者身上获得 iPSC 株系（KAIMRCi003A、KAIMRCi003B）。

IF 4.3 3区生物学

Human Cell Pub Date : 2023-12-19 DOI: 10.1007/s13577-023-01016-z

Maryam Alowaysi, Mohammad Al-Shehri, Amani Badkok, Hanouf Attas, Doaa Aboalola, Moayad Baadhaim, Hajar Alzahrani, Mustafa Daghestani, Asima Zia, Khalid Al-Ghamdi, Asayil Al-Ghamdi, Samer Zakri, Sihem Aouabdi, Jesper Tegner, Khaled Alsayegh

引用次数: 0

Tissue-specific populations from amniotic fluid-derived mesenchymal stem cells manifest variant in vitro and in vivo properties 羊水间充质干细胞的组织特异性群体表现出不同的体外和体内特性

IF 4.3 3区生物学

Human Cell Pub Date : 2023-12-12 DOI: 10.1007/s13577-023-01008-z

Nengqing Liu, Yi Cheng, Ding Wang, Hongmei Guan, Diyu Chen, Juan Zeng, Dian Lu, Yuanshuai Li, Yinghong Yang, Qian Luo, Lifen Zhu, Bin Jiang, Xiaofang Sun, Bing Song

{"title":"Tissue-specific populations from amniotic fluid-derived mesenchymal stem cells manifest variant in vitro and in vivo properties","authors":"Nengqing Liu, Yi Cheng, Ding Wang, Hongmei Guan, Diyu Chen, Juan Zeng, Dian Lu, Yuanshuai Li, Yinghong Yang, Qian Luo, Lifen Zhu, Bin Jiang, Xiaofang Sun, Bing Song","doi":"10.1007/s13577-023-01008-z","DOIUrl":"https://doi.org/10.1007/s13577-023-01008-z","url":null,"abstract":"Amniotic fluid derived mesenchymal stem cells (AFMSCs), shed along the fetal development, exhibit superior multipotency and immunomodulatory properties compared to MSCs derived from other somatic tissues (e.g., bone marrow and fat). However, AFMSCs display heterogeneity due to source ambiguity, making them an underutilized stem cells source for translational clinical trials. Consequently, there is an urgent need to identify a method to purify the AFMSCs for clinical use. We found that the AFMSCs can be categorized into three distinct groups: kidney-specific AFMSCs (AFMSCs-K), lung-specific AFMSCs (AFMSCs-L), and AFMSCs with an undefined tissue source (AFMSCs-X). This classification was based on tissue-specific gene expression pattern of single cell colony. Additionally, we observed that AFMSCs-X, a minority population within the AFMSCs, exhibited the highest multipotency, proliferation, resistance to senescence and immuno-modulation. Our results showed that AFMSCs-X significantly improved survival rates and reduced bacterial colony forming units (CFU) in cecal ligation and puncture (CLP)-induced septic mice. Therefore, our study introduces a novel classification method to enhance the consistency and efficacy of AFMSCs. These subpopulations, originating from different tissue source, may offer a valuable and innovative resource of cells for regenerative medicine purposes.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":"9 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138579928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the mixed origins of the MGC-803 cell line reveals that it is a hybrid cell line derived from HeLa 对 MGC-803 细胞系的混合来源进行调查后发现，它是由 HeLa

IF 4.3 3区生物学

Human Cell Pub Date : 2023-12-11 DOI: 10.1007/s13577-023-01011-4

Meimei Yang, Jing He, Sixuan Xia, Yudong Wang, Jun Xiong, Cong Liao, Nan Li, Sanfu Qu, Chao Shen

{"title":"Investigation of the mixed origins of the MGC-803 cell line reveals that it is a hybrid cell line derived from HeLa","authors":"Meimei Yang, Jing He, Sixuan Xia, Yudong Wang, Jun Xiong, Cong Liao, Nan Li, Sanfu Qu, Chao Shen","doi":"10.1007/s13577-023-01011-4","DOIUrl":"https://doi.org/10.1007/s13577-023-01011-4","url":null,"abstract":"Human cancer cell lines have an essential role in cancer research, but only authentic cell lines should be used as biological models. Authentication testing using short tandem repeat (STR) loci has shown that MGC-803 cells, which were reported to come from gastric adenocarcinoma, are similar to HeLa. In this study, we confirmed that the MGC-803 cell line contains genetic material from HeLa, including genetic sequence from human papilloma virus 18 (HPV18). Additional alleles were present on STR analysis that remained stable after extensive passaging and generation of mono-clones. This behavior is consistent with a hybrid cell line arising from cell–cell fusion. Further genetic analysis revealed that MGC-803 originated from donors with different genetic ancestries, one African (HeLa) and the other Asian. Transcriptomic analysis demonstrated that MGC-803 closely resembles HeLa and another nasopharyngeal–HeLa hybrid cell line CNE-2. Based on these findings, we conclude that MGC-803 is a hybrid cell line derived from HeLa and other cells, the latter derived from a different patient with Asian genetic ancestry.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":"60 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138566954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of a patient-derived solitary fibrous tumor/hemangiopericytoma cell line model 建立患者来源的单发纤维瘤/血管组织细胞瘤细胞系模型并确定其特征

IF 4.3 3区生物学

Human Cell Pub Date : 2023-12-09 DOI: 10.1007/s13577-023-01013-2

Jing Yi Lee, Peiyong Guan, Abner Herbert Lim, Zexi Guo, Zhimei Li, Jessica Sook Ting Kok, Elizabeth Chun Yong Lee, Boon Yee Lim, Bavani Kannan, Jui Wan Loh, Cedric Chuan-Young Ng, Kah Suan Lim, Bin Tean Teh, Tun Kiat Ko, Jason Yongsheng Chan

{"title":"Establishment and characterization of a patient-derived solitary fibrous tumor/hemangiopericytoma cell line model","authors":"Jing Yi Lee, Peiyong Guan, Abner Herbert Lim, Zexi Guo, Zhimei Li, Jessica Sook Ting Kok, Elizabeth Chun Yong Lee, Boon Yee Lim, Bavani Kannan, Jui Wan Loh, Cedric Chuan-Young Ng, Kah Suan Lim, Bin Tean Teh, Tun Kiat Ko, Jason Yongsheng Chan","doi":"10.1007/s13577-023-01013-2","DOIUrl":"https://doi.org/10.1007/s13577-023-01013-2","url":null,"abstract":"Solitary fibrous tumor/Hemangiopericytoma (SFT/HPC) is a rare subtype of soft tissue sarcoma harboring NAB2–STAT6 gene fusions. Mechanistic studies and therapeutic development on SFT/HPC are impeded by scarcity and lack of system models. In this study, we established and characterized a novel SFT/HPC patient-derived cell line (PDC), SFT-S1, and screened for potential drug candidates that could be repurposed for the treatment of SFT/HPC. Immunohistochemistry profiles of the PDC was consistent with the patient’s tumor sample (CD99+/CD34+/desmin−). RNA sequencing, followed by Sanger sequencing confirmed the pathognomonic NAB2exon3–STAT6exon18 fusion in both the PDC and the original tumor. Transcriptomic data showed strong enrichment for oncogenic pathways (epithelial–mesenchymal transition, FGF, EGR1 and TGFβ signaling pathways) in the tumor. Whole genome sequencing identified potentially pathogenic somatic variants such as MAGEA10 and ABCA2. Among a panel of 14 targeted agents screened, dasatinib was identified to be the most potent small molecule inhibitor against the PDC (IC50, 473 nM), followed by osimertinib (IC50, 730 nM) and sunitinib (IC50, 1765 nM). Methylation profiling of the tumor suggests that this specific variant of SFT/HPC could lead to genome-wide hypomethylation. In conclusion, we established a novel PDC model of SFT/HPC with comprehensive characterization of its genomic, epigenomic and transcriptomic landscape, which can facilitate future preclinical studies of SFT/HPC, such as in vitro drug screening and in vivo drug testing.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":"36 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138560219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1. 修正:MicroRNA-124上调通过靶向鞘氨酸激酶1抑制骨肉瘤细胞的增殖和侵袭。

IF 4.3 3区生物学

Human Cell Pub Date : 2022-11-01 DOI: 10.1007/s13577-022-00788-0

Yan Zhou, Yanzhen Han, Zhitao Zhang, Zhe Shi, Liyuan Zhou, Xiaohong Liu, Xiaoyan Jia

引用次数: 5

Exosomal microRNA-551b-3p from bone marrow-derived mesenchymal stromal cells inhibits breast cancer progression via regulating TRIM31/Akt signaling. 骨髓源间充质基质细胞外泌体microRNA-551b-3p通过调节TRIM31/Akt信号传导抑制乳腺癌进展。

IF 4.3 3区生物学

Human Cell Pub Date : 2022-11-01 Epub Date: 2022-08-09 DOI: 10.1007/s13577-022-00753-x

Ziang Yang, Bei Xu, Sheng Wu, Weige Yang, Rongkui Luo, Shengkai Geng, Zhaochen Xin, Wen Jin, Xiong Shen, Xixi Gu, Hongwei Zhang, Hong Wang

{"title":"Exosomal microRNA-551b-3p from bone marrow-derived mesenchymal stromal cells inhibits breast cancer progression via regulating TRIM31/Akt signaling.","authors":"Ziang Yang, Bei Xu, Sheng Wu, Weige Yang, Rongkui Luo, Shengkai Geng, Zhaochen Xin, Wen Jin, Xiong Shen, Xixi Gu, Hongwei Zhang, Hong Wang","doi":"10.1007/s13577-022-00753-x","DOIUrl":"https://doi.org/10.1007/s13577-022-00753-x","url":null,"abstract":"Mesenchymal stromal cells (MSCs) play an important role in the development of human cancer. Meanwhile, exosomes released by MSCs can mediate cell-cell communication by delivering microRNAs (miRNAs/miRs). Hence, this study aimed to explore the role of bone marrow mesenchymal stromal cell (BMSC)-derived exosomal miR-551b-3p in breast cancer. In this study, we found that upregulation of miR-551b-5p suppressed the proliferation and migration and induced the apoptosis of breast cancer cells via downregulating tripartite motif-containing protein 31 (TRIM31). In addition, miR-551b-5p could be transferred from BMSCs to breast cancer cells via exosomes; BMSC-derived exosomal miR-551b-3p suppressed the proliferation and migration and promoted the apoptosis and oxidative stress of MDA-MB-231 cells via inhibiting TRIM31. Furthermore, a xenograft mouse model was used to explore the role of BMSC-derived exosomal miR-551b-3p in vivo. We found that BMSC-derived exosomal miR-551b-3p inhibited tumor growth in a mouse xenograft model of breast cancer in vivo. Collectively, these findings indicated that BMSC-derived exosomal miR-551b-3p could suppress the development of breast cancer via downregulating TRIM31. Thus, miR-551b-3p could serve as a potential target for the treatment of breast cancer.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":"35 6","pages":"1797-1812"},"PeriodicalIF":4.3,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40679711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Pirfenidone promotes the levels of exosomal miR-200 to down-regulate ZEB1 and represses the epithelial-mesenchymal transition of non-small cell lung cancer cells. 吡非尼酮促进外泌体miR-200水平下调ZEB1，抑制非小细胞肺癌细胞的上皮-间质转化。

IF 4.3 3区生物学

Human Cell Pub Date : 2022-11-01 Epub Date: 2022-08-25 DOI: 10.1007/s13577-022-00766-6

Jingjing Liu, Liming Cao, Yuanyuan Li, Pengbo Deng, Pinhua Pan, Chengping Hu, Huaping Yang

{"title":"Pirfenidone promotes the levels of exosomal miR-200 to down-regulate ZEB1 and represses the epithelial-mesenchymal transition of non-small cell lung cancer cells.","authors":"Jingjing Liu, Liming Cao, Yuanyuan Li, Pengbo Deng, Pinhua Pan, Chengping Hu, Huaping Yang","doi":"10.1007/s13577-022-00766-6","DOIUrl":"https://doi.org/10.1007/s13577-022-00766-6","url":null,"abstract":"Non-small cell lung cancer (NSCLC) is the malignancy with highest mortality and morbidity. Cancer-associated fibroblasts (CAFs) are the most abundant stromal cells in the tumor microenvironment of NSCLC. This research is performed to explore the biological functions of pirfenidone (PFD) to repress the malignant phenotypes of NSCLC cells, and its regulatory effects on exosomal microRNA-200 (exo-miR-200) derived from CAFs. In the present work, we report that, exo-miR-200 secreted by CAFs restrains the migration, invasion and epithelial-mesenchymal transition (EMT) of NSCLC cells; PFD treatment promotes the secretion of exo-miR-200 from CAFs and enhances the tumor-suppressive properties of exo-miR-200 on NSCLC cells; zinc finger E-box binding homeobox 1 (ZEB1) is identified as a target of miR-200, and PFD treatment repressed the expression of ZEB1 in NSCLC cells via inducing the expression and secretion of miR-200 in CAFs. In conclusion, PFD-induced miR-200 overexpression in CAFs inhibits ZEB1 expression in NSCLC cells, and thus decelerates the migration, invasion and EMT process. Our study may provide clues for the treatment of NSCLC.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":"35 6","pages":"1813-1823"},"PeriodicalIF":4.3,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40636217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Detection of ctDNA SNPs by nested real-time Taqman qPCR: a simulation study. 巢式实时Taqman qPCR检测ctDNA snp的模拟研究。

IF 4.3 3区生物学

Human Cell Pub Date : 2022-11-01 Epub Date: 2022-09-05 DOI: 10.1007/s13577-022-00779-1

Junbei Xiang, Qian Wan, Linfeng Xie

引用次数: 0