IF 4.3 3区生物学

Human Cell Pub Date : 2024-07-27 DOI: 10.1007/s13577-024-01109-3

Kouhei Sakurai, Tatsuya Ando, Yasuhiro Sakai, Yuichiro Mori, Satoru Nakamura, Taku Kato, Hiroyasu Ito

{"title":"PROX1 is a regulator of neuroendocrine-related gene expression in lung carcinoid","authors":"Kouhei Sakurai, Tatsuya Ando, Yasuhiro Sakai, Yuichiro Mori, Satoru Nakamura, Taku Kato, Hiroyasu Ito","doi":"10.1007/s13577-024-01109-3","DOIUrl":"https://doi.org/10.1007/s13577-024-01109-3","url":null,"abstract":"Lung neuroendocrine neoplasms (NENs) are a diverse group of tumors characterized by neuroendocrine (NE) differentiation. Among lung NENs, lung carcinoid (LC) is a rare tumor with unique characteristics. Recent research has highlighted the importance of transcription factors (TFs) in establishing gene expression programs in lung NENs such as small cell lung carcinoma. However, the TFs that control the gene expression of LC are largely unknown. In this study, we report the expression and potential function of a TF called Prospero homeobox protein1 (PROX1) in LC. Publicly available transcriptome data suggested that PROX1 was highly expressed in LC tissues, which was confirmed by immunohistochemical analysis on a tissue microarray. Knockdown of PROX1 did not impact the cellular viability of an LC-derived cell line, NCI-H727. Meanwhile, transcriptome analysis revealed that PROX1 knockdown altered the expression of genes involved in NE differentiation. ASCL1, CHGA, CALCA, and LINC00261 were suggested as downstream genes of PROX1. These findings indicate that PROX1 may play an important role in the NE identity of LC by regulating the expression of key target genes.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141777832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of TK-ALCL1: a novel NPM-ALK-positive anaplastic large-cell lymphoma cell line. TK-ALCL1：一种新型NPM-ALK阳性无性大细胞淋巴瘤细胞系的建立和特征描述。

IF 4.3 3区生物学

Human Cell Pub Date : 2024-05-16 DOI: 10.1007/s13577-024-01077-8

Prin Sungwan, Jutatip Panaampon, R. Kariya, Satoshi Kamio, Rumi Nakagawa, Toru Hirozane, Yukiko Ogura, Makoto Abe, Kaoru Hirabayashi, Yukio Fujiwara, Kazutaka Kikuta, Seiji Okada

引用次数: 0

Overexpressing Bcl-2 enhances murine chimeric antigen receptor T cell therapy against solid tumor 过表达 Bcl-2 可增强小鼠嵌合抗原受体 T 细胞治疗实体瘤的效果

IF 4.3 3区生物学

Human Cell Pub Date : 2024-05-01 DOI: 10.1007/s13577-024-01066-x

Xiaoyan Wang, Guodong Liu, Xianggang Shi, Yuxing Wang, Bo Jiang, Wei Liu, Anran Dai, Xiangzhi Zhang, Feng Yu

{"title":"Overexpressing Bcl-2 enhances murine chimeric antigen receptor T cell therapy against solid tumor","authors":"Xiaoyan Wang, Guodong Liu, Xianggang Shi, Yuxing Wang, Bo Jiang, Wei Liu, Anran Dai, Xiangzhi Zhang, Feng Yu","doi":"10.1007/s13577-024-01066-x","DOIUrl":"https://doi.org/10.1007/s13577-024-01066-x","url":null,"abstract":"Chimeric antigen receptor T (CART) cell therapy has demonstrated promising potential in the treatment of hematologic malignancies. However, its application to solid tumors is limited due to the restrictive nature of the tumor microenvironment, resulting in functional failure and poor persistence of CART cells. Overexpression of Bcl-2 in human CART cells (hCART) has been found to significantly enhance their anti-apoptotic effects both in vitro and in vivo. Nevertheless, the evaluation of hCART cells in preclinical studies has predominantly relied on immunodeficient mice xenograft tumor models, making it challenging to assess the impact of hCART cells on normal tissues and the immune system. We established a murine CART (mCART) that overexpresses Bcl-2 and targets the epidermal growth factor receptor variant III (EGFRvIII), named EGFRvIII·mCART-Bcl2. It demonstrated superior proliferation, cytotoxicity, and anti-apoptotic capabilities in vitro. In an immunocompetent mouse model of abdominal metastasis of colorectal cancer, EGFRvIII·mCART-Bcl2 exhibited improved survival of CART in the abdomen, increased tumor clearance, and significantly prolonged overall mouse survival. In summary, our study provides evidence that the introduction of Bcl-2 into mCART cells can enhance their therapeutic efficacy against solid tumors while ensuring safety.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140828662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-322-5p targeting Smurf2 regulates the TGF-β/Smad pathway to protect cardiac function and inhibit myocardial infarction. 靶向 Smurf2 的 MicroRNA-322-5p 可调节 TGF-β/Smad 通路，从而保护心脏功能并抑制心肌梗死。

IF 4.3 3区生物学

Human Cell Pub Date : 2024-04-24 DOI: 10.1007/s13577-024-01062-1

Liping Guo, Ke Li, Yan Ma, Huaimin Niu, Jun Li, Xin-hua Shao, Na Li, Yuehui Sun, Haixiong Wang

引用次数: 0

Genetic diversity among the present Japanese population: evidence from genotyping of human cell lines established in Japan. 目前日本人口的遗传多样性：从日本建立的人类细胞系基因分型中获得的证据。

IF 4.3 3区生物学

Human Cell Pub Date : 2024-04-19 DOI: 10.1007/s13577-024-01055-0

Fumio Kasai, Makoto Fukushima, Yohei Miyagi, Yukio Nakamura

引用次数: 0

Establishment and characterization of novel high mucus-producing lung tumoroids derived from a patient with pulmonary solid adenocarcinoma. 从一名肺实体腺癌患者身上提取的新型高粘液分泌肺肿瘤细胞的建立和特征描述。

IF 4.3 3区生物学

Human Cell Pub Date : 2024-04-17 DOI: 10.1007/s13577-024-01060-3

Miki Iwai, Etsuko Yokota, Yuta Ishida, T. Yukawa, Yoshio Naomoto, Y. Monobe, M. Haisa, N. Takigawa, T. Fukazawa, T. Yamatsuji

引用次数: 0

Cancer-associated fibroblasts-derived exosomal METTL3 promotes the proliferation, invasion, stemness and glutaminolysis in non-small cell lung cancer cells by eliciting SLC7A5 m6A modification. 癌症相关成纤维细胞衍生的外泌体 METTL3 通过诱导 SLC7A5 m6A 修饰，促进非小细胞肺癌细胞的增殖、侵袭、干茎和谷氨酰胺溶解。

IF 4.3 3区生物学

Human Cell Pub Date : 2024-04-16 DOI: 10.1007/s13577-024-01056-z

Yafeng Fan, Yanling Yu

引用次数: 0

Characterization of immortalized ovarian epithelial cells with BRCA1/2 mutation. 带有 BRCA1/2 基因突变的永生化卵巢上皮细胞的特征。

IF 4.3 3区生物学

Human Cell Pub Date : 2024-04-14 DOI: 10.1007/s13577-024-01064-z

H. Komatsu, Masayo Okawa, Yasuhiro Kazuki, K. Kazuki, Genki Hichiwa, Kazuto Shimoya, S. Sato, Fuminori Taniguchi, Mitsuo Oshimura, T. Harada

引用次数: 0

Polyvinylpyrrolidone can prevent oolemma lysis caused by abnormal rupture of the plasma membrane in Piezo-ICSI. 聚乙烯吡咯烷酮可防止压吸-ICSI 中因质膜异常破裂导致的卵泡裂解。

IF 4.3 3区生物学

Human Cell Pub Date : 2024-04-14 DOI: 10.1007/s13577-024-01061-2

Kohei Mizuno, Osamu Okitsu, Mako Goto, Atsuko Kusuhara, Koji Kusuhara

引用次数: 0

Pioglitazone ameliorates ischemia/reperfusion-induced acute kidney injury via oxidative stress attenuation and NLRP3 inflammasome 吡格列酮通过抑制氧化应激和NLRP3炎症小体改善缺血/再灌注诱导的急性肾损伤

IF 4.3 3区生物学

Human Cell Pub Date : 2024-04-12 DOI: 10.1007/s13577-024-01059-w

Zhenfeng Ye, Jing Zhang, Zhou Xu, Zhangwang Li, Gaomin Huang, Bin Tong, Panpan Xia, Yunfeng Shen, Honglin Hu, Peng Yu, Xiaoqing Xi

{"title":"Pioglitazone ameliorates ischemia/reperfusion-induced acute kidney injury via oxidative stress attenuation and NLRP3 inflammasome","authors":"Zhenfeng Ye, Jing Zhang, Zhou Xu, Zhangwang Li, Gaomin Huang, Bin Tong, Panpan Xia, Yunfeng Shen, Honglin Hu, Peng Yu, Xiaoqing Xi","doi":"10.1007/s13577-024-01059-w","DOIUrl":"https://doi.org/10.1007/s13577-024-01059-w","url":null,"abstract":"Acute kidney injury (AKI) induced by renal ischemia/reperfusion injury (IRI) is a severe clinical condition. ROS accumulation, antioxidant pathways deficiency, and inflammation are involved in IRI. Pioglitazone (Pio) exerts anti-inflammatory and antioxidant effects. The aim of this study was to explore the protective effects of pioglitazone against IRI-induced AKI. Pathogen-free Sprague–Dawley (SD) rats were arbitrarily divided into four groups: Sham operation group Control (CON) group, CON + Pio group, I/R + Saline group, and I/R + Pio group. In addition, HK-2 cells were subjected to hypoxia and reoxygenation to develop an H/R model for investigation of the protective mechanism of Pio. Pretreatment with pioglitazone in the model rats reduced urea nitrogen and creatinine levels, histopathological scores, and cytotoxicity after IRI. Pioglitazone treatment significantly attenuated renal cell apoptosis, decreased cytotoxicity, increased Bcl-2 expression, and downregulated Bax expression. Besides, the levels of ROS and inflammatory factors, including NLRP3, ASC, pro-IL-1β, pro-caspase-1, cleaved-caspase-1, TNF-α, IL-6, and IL-1β, in I/R rats and H/R cells were normalized by the pioglitazone treatment. Pioglitazone improved IRI-induced AKI by attenuating oxidative stress and NLRP3 inflammasome activation. Therefore, pioglitazone has the potential to serve as a novel agent for renal IRI treatment and prevention.","PeriodicalId":13228,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140560896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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