Exosomal microRNA-551b-3p from bone marrow-derived mesenchymal stromal cells inhibits breast cancer progression via regulating TRIM31/Akt signaling.

IF 4.3 3区生物学

Human Cell Pub Date : 2022-11-01 Epub Date: 2022-08-09 DOI:10.1007/s13577-022-00753-x

Ziang Yang, Bei Xu, Sheng Wu, Weige Yang, Rongkui Luo, Shengkai Geng, Zhaochen Xin, Wen Jin, Xiong Shen, Xixi Gu, Hongwei Zhang, Hong Wang

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引用次数: 4

Abstract

Mesenchymal stromal cells (MSCs) play an important role in the development of human cancer. Meanwhile, exosomes released by MSCs can mediate cell-cell communication by delivering microRNAs (miRNAs/miRs). Hence, this study aimed to explore the role of bone marrow mesenchymal stromal cell (BMSC)-derived exosomal miR-551b-3p in breast cancer. In this study, we found that upregulation of miR-551b-5p suppressed the proliferation and migration and induced the apoptosis of breast cancer cells via downregulating tripartite motif-containing protein 31 (TRIM31). In addition, miR-551b-5p could be transferred from BMSCs to breast cancer cells via exosomes; BMSC-derived exosomal miR-551b-3p suppressed the proliferation and migration and promoted the apoptosis and oxidative stress of MDA-MB-231 cells via inhibiting TRIM31. Furthermore, a xenograft mouse model was used to explore the role of BMSC-derived exosomal miR-551b-3p in vivo. We found that BMSC-derived exosomal miR-551b-3p inhibited tumor growth in a mouse xenograft model of breast cancer in vivo. Collectively, these findings indicated that BMSC-derived exosomal miR-551b-3p could suppress the development of breast cancer via downregulating TRIM31. Thus, miR-551b-3p could serve as a potential target for the treatment of breast cancer.

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骨髓源间充质基质细胞外泌体microRNA-551b-3p通过调节TRIM31/Akt信号传导抑制乳腺癌进展。

间充质间质细胞(MSCs)在人类癌症的发生发展中起着重要作用。同时，MSCs释放的外泌体可以通过传递microRNAs (miRNAs/miRs)介导细胞间通讯。因此，本研究旨在探讨骨髓间充质间质细胞(BMSC)来源的外泌体miR-551b-3p在乳腺癌中的作用。在本研究中，我们发现上调miR-551b-5p通过下调TRIM31 (tripartite motif-containing protein 31)抑制乳腺癌细胞的增殖和迁移，诱导乳腺癌细胞凋亡。此外，miR-551b-5p可以通过外泌体从骨髓间充质干细胞转移到乳腺癌细胞;bmsc来源的外泌体miR-551b-3p通过抑制TRIM31抑制MDA-MB-231细胞的增殖和迁移，促进细胞凋亡和氧化应激。此外，采用异种移植小鼠模型来探索bmsc来源的外泌体miR-551b-3p在体内的作用。我们发现骨髓间充质干细胞来源的外泌体miR-551b-3p在小鼠乳腺癌异种移植模型中抑制肿瘤生长。总之，这些发现表明bmsc来源的外泌体miR-551b-3p可以通过下调TRIM31来抑制乳腺癌的发展。因此，miR-551b-3p可以作为治疗乳腺癌的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Cell 生物-细胞生物学

CiteScore

6.60

自引率

2.30%

发文量

176

期刊介绍： Human Cell is the official English-language journal of the Japan Human Cell Society. The journal serves as a forum for international research on all aspects of the human cell, encompassing not only cell biology but also pathology, cytology, and oncology, including clinical oncology. Embryonic stem cells derived from animals, regenerative medicine using animal cells, and experimental animal models with implications for human diseases are covered as well. Submissions in any of the following categories will be considered: Research Articles, Cell Lines, Rapid Communications, Reviews, and Letters to the Editor. A brief clinical case report focusing on cellular responses to pathological insults in human studies may also be submitted as a Letter to the Editor in a concise and short format. Not only basic scientists but also gynecologists, oncologists, and other clinical scientists are welcome to submit work expressing new ideas or research using human cells.