Long non-coding RNA IGFBP7-AS1 accelerates the odontogenic differentiation of stem cells from human exfoliated deciduous teeth by regulating IGFBP7 expression.

IF 4.3 3区生物学

Human Cell Pub Date : 2022-11-01 Epub Date: 2022-08-29 DOI:10.1007/s13577-022-00763-9

Dan Wang, Ningxin Zhu, Fei Xie, Man Qin, Yuanyuan Wang

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引用次数: 1

Abstract

Stem cells from human exfoliated deciduous teeth (SHED) are attractive seed cells for dental tissue engineering. We identified the effect of the long noncoding RNA insulin-like growth factor-binding protein 7 antisense RNA 1 (lncRNA IGFBP7-AS1) in vivo and its underlying mechanism during SHED odontogenic differentiation. IGFBP7-AS1 and insulin-like growth factor-binding protein 7 (IGFBP7) were overexpressed using lentiviruses. IGFBP7 expression was knocked down with small interfering RNA. The effect of IGFBP7-AS1 in vivo was confirmed by animal experiments. The effect of IGFBP7 on SHED odontogenic differentiation was assessed with alkaline phosphatase staining, alizarin red S staining, quantitative reverse transcription-PCR, and western blotting. The relationship between IGFBP7-AS1 and IGFBP7 was confirmed by quantitative reverse transcription-PCR and western blotting. IGFBP7-AS1 promoted SHED odontogenesis in vivo, and regulated the expression of the coding gene IGFBP7 positively. Inhibiting IGFBP7 led to suppress SHED odontogenic differentiation while IGFBP7 overexpression had the opposite effect. IGFBP7-AS1 enhanced the stability of IGFBP7. IGFBP7-AS1 promoted SHED odontogenic differentiation in vivo. The underlying mechanism may involve the enhancement of IGFBP7 stability. This may provide novel potential targets for dental tissue engineering.

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长链非编码RNA IGFBP7- as1通过调节IGFBP7的表达加速人脱落乳牙干细胞的成牙性分化。

人脱落乳牙干细胞是一种极具应用前景的牙组织工程种子细胞。我们确定了长链非编码RNA胰岛素样生长因子结合蛋白7反义RNA 1 (lncRNA IGFBP7-AS1)在体内的作用及其在SHED牙源性分化中的潜在机制。IGFBP7- as1和胰岛素样生长因子结合蛋白7 (IGFBP7)通过慢病毒过表达。小干扰RNA敲低IGFBP7的表达。动物实验证实了IGFBP7-AS1在体内的作用。采用碱性磷酸酶染色、茜素红S染色、定量逆转录pcr和western blotting检测IGFBP7对SHED成牙分化的影响。IGFBP7- as1与IGFBP7之间的关系通过定量逆转录pcr和western blotting证实。IGFBP7- as1在体内促进SHED牙形成，并积极调节编码基因IGFBP7的表达。抑制IGFBP7可抑制SHED牙源性分化，而过表达IGFBP7则相反。IGFBP7- as1增强了IGFBP7的稳定性。IGFBP7-AS1在体内促进SHED牙源性分化。其潜在机制可能与IGFBP7稳定性的增强有关。这可能为口腔组织工程提供新的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Cell 生物-细胞生物学

CiteScore

6.60

自引率

2.30%

发文量

176

期刊介绍： Human Cell is the official English-language journal of the Japan Human Cell Society. The journal serves as a forum for international research on all aspects of the human cell, encompassing not only cell biology but also pathology, cytology, and oncology, including clinical oncology. Embryonic stem cells derived from animals, regenerative medicine using animal cells, and experimental animal models with implications for human diseases are covered as well. Submissions in any of the following categories will be considered: Research Articles, Cell Lines, Rapid Communications, Reviews, and Letters to the Editor. A brief clinical case report focusing on cellular responses to pathological insults in human studies may also be submitted as a Letter to the Editor in a concise and short format. Not only basic scientists but also gynecologists, oncologists, and other clinical scientists are welcome to submit work expressing new ideas or research using human cells.