Investigation of the mixed origins of the MGC-803 cell line reveals that it is a hybrid cell line derived from HeLa

IF 4.3 3区生物学

Human Cell Pub Date : 2023-12-11 DOI:10.1007/s13577-023-01011-4

Meimei Yang, Jing He, Sixuan Xia, Yudong Wang, Jun Xiong, Cong Liao, Nan Li, Sanfu Qu, Chao Shen

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Abstract

Human cancer cell lines have an essential role in cancer research, but only authentic cell lines should be used as biological models. Authentication testing using short tandem repeat (STR) loci has shown that MGC-803 cells, which were reported to come from gastric adenocarcinoma, are similar to HeLa. In this study, we confirmed that the MGC-803 cell line contains genetic material from HeLa, including genetic sequence from human papilloma virus 18 (HPV18). Additional alleles were present on STR analysis that remained stable after extensive passaging and generation of mono-clones. This behavior is consistent with a hybrid cell line arising from cell–cell fusion. Further genetic analysis revealed that MGC-803 originated from donors with different genetic ancestries, one African (HeLa) and the other Asian. Transcriptomic analysis demonstrated that MGC-803 closely resembles HeLa and another nasopharyngeal–HeLa hybrid cell line CNE-2. Based on these findings, we conclude that MGC-803 is a hybrid cell line derived from HeLa and other cells, the latter derived from a different patient with Asian genetic ancestry.

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对 MGC-803 细胞系的混合来源进行调查后发现，它是由 HeLa

人类癌症细胞系在癌症研究中发挥着重要作用，但只有真实的细胞系才能被用作生物模型。使用短串联重复（STR）位点进行的鉴定测试表明，据报道来自胃腺癌的 MGC-803 细胞与 HeLa 相似。在本研究中，我们证实 MGC-803 细胞系含有来自 HeLa 的遗传物质，包括人类乳头状瘤病毒 18（HPV18）的遗传序列。在 STR 分析中还发现了其他等位基因，这些等位基因在经过大量传代和单克隆产生后仍然保持稳定。这种行为与细胞-细胞融合产生的杂交细胞系一致。进一步的遗传分析表明，MGC-803 源自不同基因血统的供体，一个是非洲人（HeLa），另一个是亚洲人。转录组分析表明，MGC-803 与 HeLa 和另一种鼻咽-HeLa 杂交细胞株 CNE-2 非常相似。基于这些研究结果，我们得出结论：MGC-803 是一种杂交细胞系，它来源于 HeLa 和其他细胞，后者来源于另一位具有亚洲遗传血统的患者。

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来源期刊

Human Cell 生物-细胞生物学

CiteScore

6.60

自引率

2.30%

发文量

176

期刊介绍： Human Cell is the official English-language journal of the Japan Human Cell Society. The journal serves as a forum for international research on all aspects of the human cell, encompassing not only cell biology but also pathology, cytology, and oncology, including clinical oncology. Embryonic stem cells derived from animals, regenerative medicine using animal cells, and experimental animal models with implications for human diseases are covered as well. Submissions in any of the following categories will be considered: Research Articles, Cell Lines, Rapid Communications, Reviews, and Letters to the Editor. A brief clinical case report focusing on cellular responses to pathological insults in human studies may also be submitted as a Letter to the Editor in a concise and short format. Not only basic scientists but also gynecologists, oncologists, and other clinical scientists are welcome to submit work expressing new ideas or research using human cells.