Horticulture Research最新文献_第5页

Sonic-induced cellular vibrations unzip intertwined anther cone trichomes to trigger floral self-pollination and boost tomato fruit size

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-14 DOI: 10.1093/hr/uhaf053

Sidra Anwar, Happy Happy, Angus Dingley, Thailammai Vinoth, Weiguang Liang, Brian M Sindel, Laurel George, Chun H Wang, Christopher I Cazzonelli

{"title":"Sonic-induced cellular vibrations unzip intertwined anther cone trichomes to trigger floral self-pollination and boost tomato fruit size","authors":"Sidra Anwar, Happy Happy, Angus Dingley, Thailammai Vinoth, Weiguang Liang, Brian M Sindel, Laurel George, Chun H Wang, Christopher I Cazzonelli","doi":"10.1093/hr/uhaf053","DOIUrl":"https://doi.org/10.1093/hr/uhaf053","url":null,"abstract":"Summary Artificial tomato pollination methods rely on cellular vibrations from air displacement, electric vibration wands and trellis tapping, which have potential to spread pathogens. Bioacoustic frequencies emitted from buzzing bees to ultrasonication can vibrate plant cells without physical contact. The effects of frequency-dependent sonication on the poricidal anther cone sheath, self-pollination, seed set, and fruit size remain unclear. We engineered devices to investigate the frequency-dependent power-law behaviour of floral living cells from greenhouse-grown tomato varieties - contrasting contact-induced oscillations from a vibrating wand and mechanical shaker arm with precision non-contact sonication emitted by a subwoofer speaker. The velocity and acceleration of vibrating flowers and impact on poricidal anther cone sheath cellular structures, self-pollination, and fruit development were assessed. Sonic frequencies ranging from 50 to 10 000 Hz increased pollination, fruit size, weight, and seed set in Sweetelle, Endeavour, Paulanca and Managua commercial varieties. Scanning electron microscopy revealed sonication separated the intertwined trichomes and unzipped their meshed network that locks the lobes of the anther cone sheath together thereby releasing pollen grains. Near ultra-sonic frequencies boosted fruit size, whereas seed set remained constant thereby challenging the floral cell power-law rheological characteristics under different frequency scales. Tomato flowers displayed a low power-law cell behaviour to frequency-dependent sonication enabling its effectiveness as a precision non-contact technology to boost pollination and tomato fruit size without a substrate-borne component.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"13 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal dynamics and tissue-specific variations of the blueberry phyllosphere mycobiome

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-12 DOI: 10.1093/hr/uhaf042

Shay Lychen Szymanski, Timothy David Miles

{"title":"Temporal dynamics and tissue-specific variations of the blueberry phyllosphere mycobiome","authors":"Shay Lychen Szymanski, Timothy David Miles","doi":"10.1093/hr/uhaf042","DOIUrl":"https://doi.org/10.1093/hr/uhaf042","url":null,"abstract":"Highbush blueberry (Vaccinium corymbosum) is an economically important fruit-bearing woody perennial. Despite the importance of microbial communities to plant health, the above-ground (phyllosphere) microbiome of blueberry is understudied. The phyllosphere is exposed to varying conditions throughout a growing season. The fruit undergoes extensive physiological change across a season from bud to fruit. This study aimed to provide a temporal characterization of the blueberry phyllosphere across a growing season and a characterization of specific tissues and phenological stages. Blueberry branches were harvested every other week across two years and two locations during the development process of the blueberry fruits. The internal transcribed spacer regions were amplified from DNA extracts and sequenced to perform amplicon-based characterization of the fungal microbiome across time and plant tissue. Fungal communities showed changes in α-diversity depending on the week of harvest and tissue type. Early in the season, α-diversity was high, but it decreased in midseason when flowers developed into fruit. Later in the season, as the fruit ripened, α-diversity increased again. The β-diversity of the community changed across time and tissue types during plant development. Notable members of the identified core microbiome were members of the genus Alternaria, Peltaster, and Taphrina, as well as the pathogenic taxa Aureobasidium pullulans and Botrytis cinerea. This research provides background for future experimentation of understanding the microbial composition in the blueberry phyllosphere in relation to the infection court of pathogens (e.g., Colletotrichum fioriniae and Botrytis cinerea) and the temporal components of blueberry plant health and management.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"15 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaping the future of bananas: advancing genetic trait regulation and breeding in the post-genomics era

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-12 DOI: 10.1093/hr/uhaf044

Hongxia Miao, Jianbin Zhang, Yunke Zheng, Caihong Jia, Yulin Hu, Jingyi Wang, Jing Zhang, Peiguang Sun, Zhiqiang Jin, Yongfeng Zhou, Sijun Zheng, Wei Wang, Mathieu Rouard, Jianghui Xie, Juhua Liu

{"title":"Shaping the future of bananas: advancing genetic trait regulation and breeding in the post-genomics era","authors":"Hongxia Miao, Jianbin Zhang, Yunke Zheng, Caihong Jia, Yulin Hu, Jingyi Wang, Jing Zhang, Peiguang Sun, Zhiqiang Jin, Yongfeng Zhou, Sijun Zheng, Wei Wang, Mathieu Rouard, Jianghui Xie, Juhua Liu","doi":"10.1093/hr/uhaf044","DOIUrl":"https://doi.org/10.1093/hr/uhaf044","url":null,"abstract":"(Musa spp.) are among the top-produced food crops, serving as a primary source of food for millions of people. Cultivated bananas originated primarily from the wild diploid species Musa acuminata (A genome) and Musa balbisiana (B genome) through intra- and inter-specific hybridization and selections via somatic variation. Following the publication of complete A- and B-genome sequences, prospects for complementary studies on S- and T-genome traits, key gene identification for yield, ripening, quality, and stress resistance, and advances in molecular breeding have significantly expanded. In this review, latest research progress on banana A, B, S, and T genomes is briefly summarized, highlighting key advances in banana cytoplasmic inheritance, flower and fruit development, sterility, and parthenocarpy, postharvest ripening and quality regulation, and biotic and abiotic stress resistance associated with desirable economic traits. We provide updates on transgenic, gene editing, and molecular breeding. We also explore future directions for banana breeding and genetic improvement.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"50 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporally transcriptomic analyses of floral buds reveal the high-resolution landscape of flower development and dormancy regulation in peach 花蕾时空转录组分析揭示了桃花发育和休眠调控的高分辨率图谱

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-11 DOI: 10.1093/hr/uhaf029

Ya-lin Zhao, Yong Li, Dan-dan Guo, Xue-jia Chen, Ke Cao, Jin-long Wu, Wei-chao Fang, Chang-wen Chen, Xin-wei Wang, Li-rong Wang

{"title":"Spatiotemporally transcriptomic analyses of floral buds reveal the high-resolution landscape of flower development and dormancy regulation in peach","authors":"Ya-lin Zhao, Yong Li, Dan-dan Guo, Xue-jia Chen, Ke Cao, Jin-long Wu, Wei-chao Fang, Chang-wen Chen, Xin-wei Wang, Li-rong Wang","doi":"10.1093/hr/uhaf029","DOIUrl":"https://doi.org/10.1093/hr/uhaf029","url":null,"abstract":"The spatiotemporal transcriptome dataset reported here provides the peach flower bud’s gene expression atlas at spatiotemporal resolution level by 10x genomics Visium platform. This dataset can be used to define the transcript accumulation for any interesting genes across several flower bud cells by three peach flower bud samples during the activity-dormancy period, providing valuable insight into gene expression profiling and developmental stages under different environmental contexts or conditions. Importantly, we found that different cell types are related to specific regulatory programs, including signal transduction, environment and stress responses, and flower development. Our research provides insight into the transcriptomic landscape of the key cell types for flower buds and opens new avenues to study cell-type specification, function, and differentiation in Rosaceae fruit trees. A series of pivotal genes (e.g., AMS, MS188, MS1) for flower bud development were identified, these works provided a valuable reference for activity-dormancy transition in perennial deciduous fruit trees.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"14 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-silencing of PhENO1 and PhPPT alters anthocyanin production by reducing phosphoenolpyruvate supply in petunia flower

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-11 DOI: 10.1093/hr/uhaf040

Xin Li, Jiahao Cao, Guiyun Jiang, Wenqi Deng, Huimin Deng, Weiyuan Yang, Yixun Yu, Juanxu Liu

{"title":"Co-silencing of PhENO1 and PhPPT alters anthocyanin production by reducing phosphoenolpyruvate supply in petunia flower","authors":"Xin Li, Jiahao Cao, Guiyun Jiang, Wenqi Deng, Huimin Deng, Weiyuan Yang, Yixun Yu, Juanxu Liu","doi":"10.1093/hr/uhaf040","DOIUrl":"https://doi.org/10.1093/hr/uhaf040","url":null,"abstract":"The shikimate pathway is crucial for the production of aromatic amino acids and various secondary plant products, including anthocyanins. Phosphoenolpyruvate (PEP) is an important source for shikimate production. The pre-chorismate part of the shikimate pathway is confined to plastids. There are three sources of PEP in plastids. PEP can be imported into the plastids from cytoplasm via the PEP/phosphate translocator (PPT), and it can also be generated in plastids via enolase (ENO) and pyruvate orthophosphate dikinase (PPDK) catalysis. A large number of anthocyanins are synthesized in the flowers of most ornamental plants in the coloring stage. However, the source of PEP, the precursor of anthocyanin synthesis, is still unknown. Herein, Petunia hybrida PhENO1, PhPPT and PhPPDK genes were identified and their subcellular localization and expression patterns were analyzed. Silencing PhENO1, PhPPT and PhPPDK alone and co-silencing of PhENO1 and PhPPDK or PhPPT and PhPPDK did not exhibit any visible phenotypic change compared with the control, while co-silencing of PhENO1 and PhPPT resulted in the flower color change from purple to light purple. The content of PEP, shikimate, flavonoids, anthocyanins and aromatic amino acids were all significantly decreased in PhENO1 and PhPPT co-silenced plants. Co-silencing PhENO1 and PhPPT did not affect the expression level of key genes in anthocyanin synthesis and shikimate pathways. Furthermore, co-silencing of PhENO1, PhPPT and PhPPDK resulted in a phenotype similar to the co-silencing of PhENO1 and PhPPT. Altogether, our study suggested that PEP used for anthocyanin synthesis is mainly provided by PhENO1 and PhPPT, rather than PhPPDK.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"6 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Floral scent emission of Epiphyllum oxypetalum: discovery of its cytosol-localized geraniol biosynthesis

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-11 DOI: 10.1093/hr/uhaf039

Yiyang Zhang, Yuhan Zhang, Andong Zhang, Qiurui Tian, Bin Yang, Likun Wei, Wei Wu, Ting Zhu, Zhiwei Zhou, Jiaqi Wang, Zhibin Liu, Wei Tang, Haijun Xiao, Mingchun Liu, Tao Li, Qun Sun

{"title":"Floral scent emission of Epiphyllum oxypetalum: discovery of its cytosol-localized geraniol biosynthesis","authors":"Yiyang Zhang, Yuhan Zhang, Andong Zhang, Qiurui Tian, Bin Yang, Likun Wei, Wei Wu, Ting Zhu, Zhiwei Zhou, Jiaqi Wang, Zhibin Liu, Wei Tang, Haijun Xiao, Mingchun Liu, Tao Li, Qun Sun","doi":"10.1093/hr/uhaf039","DOIUrl":"https://doi.org/10.1093/hr/uhaf039","url":null,"abstract":"Epiphyllum oxypetalum, a renowned ornamental species in Cactaceae, releases attractive fragrance during its infrequent, transient and nocturnal blooms. However, the floral fragrance composition and biosynthesis remain largely unexplored. Employing volatilomics, transcriptomics and biochemistry, we systematically characterized the composition, emission dynamics and biosynthesis of the floral scent of E. oxypetalum. The floral scent composition of E. oxypetalum was highly dynamic. Starting after 20:00 local time, volatile emission increased 200-fold within six hours. At full bloom, geraniol accounted for 72.54% of the total emission, followed by benzyl alcohol (12.96%) and methyl salicylate (3.75%). These scents predominantly originated from petals and sepals. Transcriptomic analysis and inhibition assays using pathway-specific inhibitors revealed that the mevalonate pathway was the precursor source for geraniol biosynthesis. Functionally characterized cytosol localizing geraniol synthase EoTPSa1 was the key enzyme responsible for geraniol biosynthesis. Together, these findings pinpointed a cytosolic biosynthetic route for the major scent volatile geraniol in E. oxypetalum. Our study provides new insights into the emission dynamics and biosynthesis of E. oxypetalum floral scents. In particular, we demonstrated a distinctive mevalonate pathway-based geraniol biosynthetic pathway, which may hold potential for the development of novel perfume products.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"129 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Litchi40K v1.0: a cost-effective, flexible and versatile liquid SNP chip for genetic analysis and digitalization of germplasm resources in litchi

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-10 DOI: 10.1093/hr/uhaf038

Lei Zhang, Pengfei Wang, Fang Li, Li Xu, Jietang Zhao, Jingxiao Fu, Jiabin Wang, Hui Zhang, Songang Li, Jiwang Hong, Jian Zheng, Xinping Luo, Huanling Li, Jiabao Wang

{"title":"Litchi40K v1.0: a cost-effective, flexible and versatile liquid SNP chip for genetic analysis and digitalization of germplasm resources in litchi","authors":"Lei Zhang, Pengfei Wang, Fang Li, Li Xu, Jietang Zhao, Jingxiao Fu, Jiabin Wang, Hui Zhang, Songang Li, Jiwang Hong, Jian Zheng, Xinping Luo, Huanling Li, Jiabao Wang","doi":"10.1093/hr/uhaf038","DOIUrl":"https://doi.org/10.1093/hr/uhaf038","url":null,"abstract":"Genetic breeding and molecular identification in varieties depend on high-performance genotyping tools. The high heterozygosity of the litchi genome contributes to increased resequencing costs and elevated error rates in hybridization-based genotyping methods. In this study, a liquid chip named Litchi4K v1.0 was developed with high-depth resequencing data from 875 litchi samples, and its efficacy was validated across three different populations. In the L. chinensis var. fulvosus population, three subpopulations characterized by spatial distribution, and a total of 1,110 genes were identified in the genomic regions with subpopulation differentiation. Additionally, a total of 30 significant signals associated with diverse agronomic traits were identified. The H002 haplotype of LITCHI02696, dominant in the Sub2 subgroup, significantly increased the soluble solid content in the L. chinensis var. fulvosus population. In a hybrid F1 population, a high-density genetic map was constructed and 79 dwarfing-related QTLs were identified with the liquid chip. A NAC transcription factor was identified as a candidate gene with a heterozygous frameshift variant in the male parent. To facilitate the digitization of germplasm resources, 384 SNPs were selected, and the DNA fingerprint map revealed clear genetic relationships and a total of 10 potential synonym groups or instances of bud mutations were identified in 164 main cultivated litchi varieties. This study provides cost-effective, flexible and versatile liquid chip for genetic analysis and digitalization of germplasm resources in litchi.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"63 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional characterization of genes associated with anthocyanin acylation in blueberry

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-10 DOI: 10.1093/hr/uhaf041

Molla F Mengist, Muhammad Ali Abid, Mary H Grace, Romit Seth, Nahla Bassil, Colin D Kay, Andrew P Dare, David Chagné, Richard V Espley, Andrew Neilson, Mary Ann Lila, Mario Ferruzzi, Massimo Iorizzo

{"title":"Identification and functional characterization of genes associated with anthocyanin acylation in blueberry","authors":"Molla F Mengist, Muhammad Ali Abid, Mary H Grace, Romit Seth, Nahla Bassil, Colin D Kay, Andrew P Dare, David Chagné, Richard V Espley, Andrew Neilson, Mary Ann Lila, Mario Ferruzzi, Massimo Iorizzo","doi":"10.1093/hr/uhaf041","DOIUrl":"https://doi.org/10.1093/hr/uhaf041","url":null,"abstract":"Blueberry is promoted as a super food with several health properties derived from chlorogenic acid and anthocyanin. Previous studies indicated that anthocyanin acylation and the content of chlorogenic acid could affect their level of absorption and biological activity. In this study, a genome-wide association study was performed to identify loci associated with anthocyanin and chlorogenic acid and characterize the candidate genes controlling anthocyanin acylation. Two stable loci controlling anthocyanin acylation and glucose specific glycosylation were confirmed on chromosomes 2 and 4, respectively, while no stable loci associated with chlorogenic acid were identified. Two acyl-CoA acyltransferases named VcBAHD-AT1 and VcBAHD-AT4, were identified as best candidate genes controlling anthocyanin acylation. Interestingly, the two genes clustered in acyl-CoA acyltransferases clade III, a clade that is not commonly associated with anthocyanin acylation. A Virus-induced gene silencing approach optimized for silencing VcBAHD-AT1 and VcBAHD-AT4 in the whole blueberry fruits, confirmed the role of these two genes in anthocyanin acylation. Overall, this study establishes the foundation to develop a molecular marker to select for higher acylated anthocyanin and delivered a method for rapid functional characterization of genes associated with other fruit related traits in blueberry. Also, the study adds evidence that during the evolution of acyl-CoA acyltransferases multiple routes led to the emergence and/or fixation of the anthocyanin acyltransferase activity. These outcomes advance knowledge about the genes controlling anthocyanin acylation in blueberries and that extend to other plants. Selecting new blueberry cultivars with higher acylated anthocyanin levels could potentially increase absorption of this health related bioactive.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"56 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MADS-domain transcription factor AGAMOUS LIKE-9 participates in the gibberellin pathway to promote bud dormancy release of tree peony

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-10 DOI: 10.1093/hr/uhaf043

Niu Demei, Liu Fang, Gao Linqiang, Zhang Huailong, Liu Naibin, Zhang Lu, Yuan Yanchao, Liu Chunying, Gai Shupeng, Zhang Yuxi

{"title":"MADS-domain transcription factor AGAMOUS LIKE-9 participates in the gibberellin pathway to promote bud dormancy release of tree peony","authors":"Niu Demei, Liu Fang, Gao Linqiang, Zhang Huailong, Liu Naibin, Zhang Lu, Yuan Yanchao, Liu Chunying, Gai Shupeng, Zhang Yuxi","doi":"10.1093/hr/uhaf043","DOIUrl":"https://doi.org/10.1093/hr/uhaf043","url":null,"abstract":"Bud dormancy, which serves as a survival mechanism during winter, is crucial for determining the timing and quality of flowering in many perennial woody plants, including tree peony. The gibberellin (GA) signalling pathway participates in breaking bud dormancy in tree peony. Specifically, PsRGL1, a key DELLA protein, is a negative regulator in this process. MADS-box family members participate in plant growth and development regulation. In this study, a MADS-domain transcription factor, AGAMOUS-LIKE 9 (PsAGL9), was identified as a candidate interaction protein of PsRGL1 using a pull-down assay coupled with liquid chromatography-tandem mass spectrometry. PsAGL9 expression was induced by chilling and exogenous GA3. Yeast two-hybrid (Y2H), pull-down, and luciferase complementation assays (LCAs) confirmed that PsAGL9 interacted with PsRGL1. PsAGL9 overexpression significantly promoted dormancy break and upregulated the expression of marker genes such as PsBG6, PsBG9, PsEBB1, PsEBB3, and PsCYCD, suggesting a potential regulatory function of PsAGL9. Classical and non-classical CArG motifs were identified in the promoter regions of PsCYCD and PsEBB3, respectively. Yeast one-hybrid, electrophoretic mobility shift, and dual luciferase assays confirmed that PsAGL9 directly bound to and activated PsCYCD and PsEBB3 expression, and PsRGL1 abolished the DNA-binding activity of PsAGL9. Furthermore, interaction proteins of PsAGL9 were screened, and MADS-box members PsAGL9, PsAGL6, and PsPI were identified. Y2H, LCA, and pull-down assays confirmed that PsAGL9 formed both homodimers and heterodimers, and heterodimers further promoted target gene expression. This study provides an in-depth exploration of the GA pathway and elucidates a novel pathway, PsRGL1-PsAGL9-PsCYCD, involved in regulating dormancy break in tree peony.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"167 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wo interacts with SlTCP25 to regulate type I trichome branching in tomato

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-05 DOI: 10.1093/hr/uhaf032

Junqiang Wang, Shoujuan Yuan, Yihao Zhao, Xin Shu, Zhiling Liu, Taotao Wang, Zhibiao Ye, Changxian Yang

{"title":"Wo interacts with SlTCP25 to regulate type I trichome branching in tomato","authors":"Junqiang Wang, Shoujuan Yuan, Yihao Zhao, Xin Shu, Zhiling Liu, Taotao Wang, Zhibiao Ye, Changxian Yang","doi":"10.1093/hr/uhaf032","DOIUrl":"https://doi.org/10.1093/hr/uhaf032","url":null,"abstract":"Plant trichomes serve as a protective barrier against various stresses. Although the molecular mechanisms governing the initiation of trichomes have been extensively studied, the regulatory pathways underlying the trichome branching in tomato remain elusive. Here, we found that Woolly (Wo) mutant and its overexpression transgenic plants displayed branched type I trichomes. The expression level of SlTCP25, a transcription factor of type TB1 of the TCP subfamily, was obviously decreased in Wo mutant and Wo overexpressing lines. Knockout of SlTCP25 resulted in the formation of type I trichome branches on the hypocotyls. Genetic evidence showed that SlTCP25 is epistatic to Wo in the branched trichome formation. Biochemical data further indicated that Wo can directly bind to the L1-box cis-element in the SlTCP25 promoter and repress its transcription. We further determined that SlTCP25 interacts with Wo to weaken Wo-regulated the expression of SlCycB2, a trichome branching inhibitor. In addition, the number of trichome branches was significantly increased in Sltcp25Slcycb2 double mutant, suggesting that SlTCP25 and SlCycB2 coordinately repress trichome branching in wild type. In conclusion, we elucidate a molecular network governing the morphogenesis of multicellular trichomes in tomato.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"31 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143125112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0