Plant resistance inducer AMHA enhances antioxidant capacities to promote cold tolerance by regulating the upgrade of glutathione S-transferase in tea plant

IF 8.7 1区农林科学 Q1 Agricultural and Biological Sciences

Horticulture Research Pub Date : 2025-03-05 DOI:10.1093/hr/uhaf073

Xuejin Chen, Ning Zhou, Lisha Yu, Zhaolan Han, Yanjing Guo, Salome Njeri Ndombi, Huan Zhang, Jie Jiang, Yu Duan, Zhongwei Zou, Yuanchun Ma, Xujun Zhu, Shiguo Chen, Wanping Fang

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引用次数: 0

Abstract

Plant resistance inducers represent an alternative strategy that mitigate stress-induced damage in plants. Previously, 2-Amino-3-methylhexanoic Acid (AMHA), a novel natural plant resistance inducer, was shown to significantly bolster cold tolerance, thermotolerance and pathogen resistance in plants. However, the intricate mechanisms underlying AMHA’s response to cold stress remain elusive. Thus, we investigated the physiological and transcriptomic analyses of AMHA pretreatment on tea plant to determine the substantial role of AMHA under cold stress. The results showed that pretreatment with 100 nM AMHA effectively mitigated the detrimental effects of cold stress on photosynthesis and growth. Furthermore, differentially expressed genes were identified through RNA-seq during pretreatment, cold stress, and 2 days (2 d) of recovery. These genes were mainly enriched in pathways related to flavonoid/anthocyanin, carotenoid, and ascorbic acid-glutathione (AsA-GSH) cycle, including GST (encoding glutathione S-transferase). Potential regulatory relationships between the identified genes and transcription factors were also established. Antisense oligodeoxynucleotide-silencing and overexpression experiments revealed that CsGSTU7 enhances cold resistance by maintaining redox homeostasis. In conclusion, our study suggests that antioxidant-related signal molecules play a critical role in the signal cascades and transcriptional regulation mediating AMHA-induced cold-stress resistance in tea plant.

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植物抗性诱导剂AMHA通过调节茶树谷胱甘肽s -转移酶的升级来增强抗氧化能力，促进茶树的耐寒性

植物抗性诱导剂是缓解植物应力诱导损伤的另一种策略。2-氨基-3-甲基己酸（AMHA）是一种新型的天然植物抗性诱导剂，具有显著的植物抗寒性、抗热性和抗病性。然而，AMHA对冷应激反应的复杂机制仍然难以捉摸。因此，我们研究了AMHA预处理对茶树的生理和转录组学分析，以确定AMHA在冷胁迫下的实质性作用。结果表明，100 nM AMHA预处理能有效缓解冷胁迫对水稻光合和生长的不利影响。此外，在预处理、冷胁迫和恢复2天（2 d）时，通过RNA-seq鉴定差异表达基因。这些基因主要富集于与类黄酮/花青素、类胡萝卜素和抗坏血酸-谷胱甘肽（asas - gsh）循环相关的途径，包括编码谷胱甘肽s -转移酶的GST。鉴定的基因与转录因子之间的潜在调控关系也被确立。反义寡脱氧核苷酸沉默和过表达实验表明，CsGSTU7通过维持氧化还原稳态来增强抗寒能力。综上所述，我们的研究表明，抗氧化相关信号分子在介导amha诱导的茶树抗冷胁迫的信号级联和转录调控中发挥了关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

11.20

自引率

6.90%

发文量

367

审稿时长

20 weeks

期刊介绍： Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.