Horticulture Research最新文献

OfWRKY33 binds to the promoter of key linalool synthase gene OfTPS7 to stimulte linalool synthesis in Osmanthus fragrans flowers OfWRKY33结合关键的芳樟醇合成酶基因OfTPS7的启动子，刺激桂花中芳樟醇的合成

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-16 DOI: 10.1093/hr/uhaf155

Wan Xi, Meng-Yu Jiang, Lin-lin Zhu, Xu-Mei Zeng, Huan Ju, Qing-Lian Yang, Ting-Yu Zhang, Cai-Yun Wang, Ri-Ru Zheng

{"title":"OfWRKY33 binds to the promoter of key linalool synthase gene OfTPS7 to stimulte linalool synthesis in Osmanthus fragrans flowers","authors":"Wan Xi, Meng-Yu Jiang, Lin-lin Zhu, Xu-Mei Zeng, Huan Ju, Qing-Lian Yang, Ting-Yu Zhang, Cai-Yun Wang, Ri-Ru Zheng","doi":"10.1093/hr/uhaf155","DOIUrl":"https://doi.org/10.1093/hr/uhaf155","url":null,"abstract":"Volatile aroma compounds make significant contributions to human perception of flowers. Osmanthus fragrans is a famous aroma plant and linalool is proved to be the dominant aroma active compound. Although some terpene synthases (TPSs) have been characterized, a comprehensive study of the hub metabolic gene and its transcriptional regulation remain to be revealed. Here, we selected a specific cultivar Boyeyingui (BBYG) with the highest content of linalool among 20 wide-cultivated cultivars for genome and transcriptome sequencings. Among the 25 new putative OfTPSs, only OfTPS6, OfTPS7 could exclusively produce linalool in planta. Biochemical analysis demonstrated that OfTPS6, OfTPS7 were able to catalyze geranyl diphosphate (GPP) into linalool and a small proportion of other monoterpenes in vitro. Spatial and temporal correlation analysis further confirmed the expression level of OfTPS7 was strongly correlated with linalool content in a panel of 20 cultivars, suggesting OfTPS7 was the essential linalool synthase gene. Combined with yeast one-hybrid screen and weighted correlation network analysis (WGCNA), a nucleus-localized transcriptional factor OfWRKY33 was identified as a prospective modulator. Y1H, LUC and EMSA demonstrated that OfWRKY33 directly bound to the W-box of OfTPS7 promoter to stimulate its transcription. OfWRKY33 could coordinately induce the expressions of OfTPS7 and 1-deoxy-d-xylulose 1 (OfDXS1), thereby promoting the linalool formation. The results first identified the key linalool synthase gene OfTPS7 and a novel transcription factor playing a role in the complex regulatory network of linalool biosynthesis in O. fragrans flowers.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"143 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144296060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin in Plant Pathogen Defense: A Review of Its Role in Horticultural Crops 褪黑素在植物病原体防御中的作用：在园艺作物中的研究进展

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-11 DOI: 10.1093/hr/uhaf150

Xinyi Hao, Jinyu Ren, Mingyuan Xu, Binghui Sun, Rui Li, Shijin Yang, Weirong Xu

{"title":"Melatonin in Plant Pathogen Defense: A Review of Its Role in Horticultural Crops","authors":"Xinyi Hao, Jinyu Ren, Mingyuan Xu, Binghui Sun, Rui Li, Shijin Yang, Weirong Xu","doi":"10.1093/hr/uhaf150","DOIUrl":"https://doi.org/10.1093/hr/uhaf150","url":null,"abstract":"Horticultural crops have important economic value in the world. Biotic stress has serious impacts on horticultural crops’ growth and development as well as yield. Melatonin, a multifunctional signaling molecule, has been increasingly documented to play a pivotal role in mediating plant defense responses against diverse biotic stressors, including bacterial, fungal, and viral pathogens in horticultural crop species. Previous studies showed that exogenous melatonin treatment significantly improved horticultural crops growth and increased their tolerance to biotic stress. Although there are numerous studies to show that exogenous melatonin treatment can markedly improve the tolerance for horticultural crops in response to biotic stress, the role of melatonin in biotic stress responses remains unclear and requires clarification. In the review, we summarize the effects of melatonin on horticultural crops’ disease resistance. Moreover, we assess future perspectives in melatonin research and its applications to improve horticultural crop production and tolerance for biotic stress. This review explores future research directions and potential applications to enhance the productivity and biotic stress tolerance of horticultural crops, and also provides a theoretical basis for enhancing the scientific understanding of the role of melatonin in response to biotic stress in horticultural crops.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"47 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144260138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Free fatty acid biosynthesis precursors are involved in pollen-stigma interactions in Brassica 游离脂肪酸生物合成前体参与了芸苔属花粉-柱头相互作用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-11 DOI: 10.1093/hr/uhaf147

Hongtao Qin, Kumar Abhinandan, Min Wang, Huan Chen, Xue Zhang, Lanlan Li, Zhengwenyang Zhou, Suhui Wang, Chaoning Zhao, Wendi Mu, Yongxue Yuan, Ying Wu, Yuhua Li, Marcus A Samuel, Xingguo Lan

{"title":"Free fatty acid biosynthesis precursors are involved in pollen-stigma interactions in Brassica","authors":"Hongtao Qin, Kumar Abhinandan, Min Wang, Huan Chen, Xue Zhang, Lanlan Li, Zhengwenyang Zhou, Suhui Wang, Chaoning Zhao, Wendi Mu, Yongxue Yuan, Ying Wu, Yuhua Li, Marcus A Samuel, Xingguo Lan","doi":"10.1093/hr/uhaf147","DOIUrl":"https://doi.org/10.1093/hr/uhaf147","url":null,"abstract":"Self-incompatibility (SI) is a complex molecular mechanism in flowering plants that prevents self-fertilization and promotes outcrossing. We conducted metabolome analysis of ornamental kale (Brassica oleracea var. acephala) pistils following SI and compatible pollination (CP), revealing significant alterations in lipid metabolism, particularly the accumulation of free fatty acid (FFA) metabolites during CP. Treatment of stigmas with acetyl-CoA and malonyl-CoA, key precursors in fatty acid synthesis, broke down SI and enhanced CP. Conversely, inhibiting acetyl-CoA carboxylase (ACCase), the rate-limiting enzyme in de novo fatty acid synthesis, significantly reduced compatible pollen attachment and tube growth, highlighting the critical role of fatty acid metabolism in mediating pollination success. We identified a novel interaction between the FERONIA (BoFER) receptor kinase and the biotin carboxyl carrier protein 1 (BoBCCP1), a subunit of the ACCase complex. Suppressing the expression of BoBCCP1 in the stigma reduced CP response, suggesting that the FER-BCCP1 module may play a crucial role in regulating fatty acid biosynthesis and determining the outcome of pollen-stigma interactions. Our findings provide new insights into the identification of key metabolic pathways and signaling modules controlling pollen-stigma interactions, and offer a valuable resource for the targeted improvement of Brassica crop breeding.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"6 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome-Level Reference Genome of Vitis piasezkii var. pagnucii provides insights into a new locus of resistance to grapevine powdery mildew 白葡萄（Vitis piasezkii var. pagnucii）染色体水平参考基因组为葡萄白粉病抗性的新位点提供了新的见解

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-10 DOI: 10.1093/hr/uhaf146

Liang Zhao, Yang Hu, Qian-Yu Ji, Li-Xue Gong, Meng-Jiao Lu, Xue-Na Yu, Zhuo-Shuai Jin, Min Zhou, Xue-Lei Dai, Shun-Yuan Xiao, Yu Jiang, Ying-Qiang Wen

{"title":"Chromosome-Level Reference Genome of Vitis piasezkii var. pagnucii provides insights into a new locus of resistance to grapevine powdery mildew","authors":"Liang Zhao, Yang Hu, Qian-Yu Ji, Li-Xue Gong, Meng-Jiao Lu, Xue-Na Yu, Zhuo-Shuai Jin, Min Zhou, Xue-Lei Dai, Shun-Yuan Xiao, Yu Jiang, Ying-Qiang Wen","doi":"10.1093/hr/uhaf146","DOIUrl":"https://doi.org/10.1093/hr/uhaf146","url":null,"abstract":"Grapevine powdery mildew (GPM), caused by Erysiphe necator, poses a significant threat to all green grapevine tissues, leading to substantial economic losses in viticulture. Traditional grapevine cultivars derived from Vitis vinifera are highly susceptible to GPM, whereas the wild Chinese accession Baishui-40 (BS-40) of V. piasezkii var. pagnucii exhibits robust resistance. To illuminate the genetic basis of resistance, we sequenced and assembled the chromosome-level genome of ‘BS-40’, achieving a total mapped length of 578.6 Mb distributed across nineteen chromosomes. A comprehensive annotation identified 897 nucleotide-binding leucine-rich repeat (NLR) genes in the ‘BS-40’ genome, which exhibited high sequence similarity across Vitis genomes. 284 of these NLR genes were differentially expressed upon GPM infection. A hybrid population of ‘BS-40’ and V. vinifera was constructed and 195 progenies were whole-genome re-sequenced. A new GPM-resistant locus, designated Ren17, located within the 0.74-1.23 Mb region on chromosome 1 was identified using genome-wide association study, population selection, and QTL analysis. Recombinant events indicated that an NLR gene cluster between 1,045,489 and 1,089,719 bp on chromosome 1 is possibly the key contributor to GPM resistance in ‘BS-40’. Based on an SNP within this region, a dCAPS marker was developed that can predict the GPM resistance in ‘BS-40’-derived materials with 99.4% accuracy in the progenies of ‘BS-40’ and V. vinifera. This chromosome-level genome assembly of V. piasezkii var. pagnucii provides a valuable resource not only for grapevine evolution, genetic analysis, and pan-genome studies but also a new locus Ren17 as a promising target for GPM-resistant breeding in grapevine.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"36 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144252307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The StUBC18-StPUB40 pair negatively regulate drought stress tolerance and influences tuber yield in potato StUBC18-StPUB40对负调控马铃薯抗旱性，影响马铃薯块茎产量

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-10 DOI: 10.1093/hr/uhaf145

Weigang Liu, Xun Tang, Rui Ma, Jiangwei Yang, Xue Fu, Huanhuan Zhang, Shigui Li, Ning Zhang, Huaijun Si

{"title":"The StUBC18-StPUB40 pair negatively regulate drought stress tolerance and influences tuber yield in potato","authors":"Weigang Liu, Xun Tang, Rui Ma, Jiangwei Yang, Xue Fu, Huanhuan Zhang, Shigui Li, Ning Zhang, Huaijun Si","doi":"10.1093/hr/uhaf145","DOIUrl":"https://doi.org/10.1093/hr/uhaf145","url":null,"abstract":"The ubiquitin-proteasome system (UPS) is important for protein post-translational modification in plants. E2 (Ubiquitin-conjugating enzyme) and E3 (Ubiquitin ligases enzyme), key enzymes of UPS, play crucial roles in all aspects of plant development, growth, and environmental stresses. Despite extensive knowledge of UPS roles in crop growth and development, E2-E3 pair functions in potato tuber development and stress responses remain understudied. Here, we describe the role of StUBC18 (a potato E2) in drought stress tolerance. It is determined that StUBC18 (E2)-StPUB40 (E3) pair plays important roles in drought stress tolerance and potato tuber yield. StUBC18 and StPUB40 expression was down-regulated under various stresses (drought, salt, polyethylene glycol, and H2O2). Over-expression of StUBC18 and StPUB40 in potatoes decreased drought stress tolerance, while interfering with the expression of StUBC18 and StPUB40 increased drought stress tolerance, respectively. The protein interaction test demonstrated that StUBC18 interacts with StPUB40 in the plant cell. Co-overexpression of StUBC18-StPUB40 in potato enhanced ROS accumulation and induced pleiotropic changes, reducing drought tolerance. Our findings revealed how the StUBC18-StPUB40 pair regulates potato drought stress tolerance by altering leaf anatomy (palisade and spongy tissue thickness) and influences tuber yield.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"2 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144252306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The BnSEP-BnTFL1s module regulates inflorescence architecture based on light duration in Brassica napus L. BnSEP-BnTFL1s模块根据光照时数调控甘蓝型油菜的花序结构。

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-09 DOI: 10.1093/hr/uhaf151

Lingxiong Zan, Xutao Zhao, Shiying Lv, Haidong Liu, Jingxiu Ye, Yanmei Yao, Kaixiang Li, Dezhi Du

{"title":"The BnSEP-BnTFL1s module regulates inflorescence architecture based on light duration in Brassica napus L.","authors":"Lingxiong Zan, Xutao Zhao, Shiying Lv, Haidong Liu, Jingxiu Ye, Yanmei Yao, Kaixiang Li, Dezhi Du","doi":"10.1093/hr/uhaf151","DOIUrl":"https://doi.org/10.1093/hr/uhaf151","url":null,"abstract":"Rapeseed (Brassica napus L.) with determinate inflorescence (DTI) exhibit desirable traits, including reduced plant height, enhanced lodging resistance, and consistent maturity, making them valuable breeding resources. DTI is modulated by BnaA10.TFL1 and BnaC09.TFL1 (BnaA10/C09.TFL1) encode the TERMINAL FLOWER 1 protein, a key regulator of flowering time and meristem identity. However, the underlying functional and regulatory mechanisms remain unclear. In this study, we demonstrated that variations in the promoter region of BnaA10/C09.TFL1, rather than the coding region, contributed to the transition from indeterminate inflorescence (IDTI) to DTI in B. napus. Specifically, BnaA10.SEP inhibited BnaA10/C09.TFL1 expression by binding to the GT1-motif in the promoter region of BnaA10/C09.TFL1DTI, contributing to the IDTI phenotype under short-day conditions. Meanwhile, two novel DTI mutants were successfully generated through the simultaneous knockout of BnaA10/C09.TFL1 using the CRISPR/Cas9 system. Furthermore, BnaA10/C09.TFL1 and its homolog BnaA02.FT interacted with BnaA07.14-3-3 instead of directly binding to BnaA08.FD to regulate the development of different inflorescence architectures. Overall, the BnaA10.SEP–BnaA10/C09.TFL1–BnaA07.14-3-3–BnaA08.FD module revealed a new mechanism for DTI formation and a promising strategy for modifying inflorescence architecture traits in B. napus.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"7 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Analysis of a UDP-Glucosyltransferase Gene Contributing to Biosynthesis of the Flavonol Tri-glycoside in Tea Plants 参与茶树黄酮醇三糖苷生物合成的udp -葡萄糖基转移酶基因的功能分析

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-09 DOI: 10.1093/hr/uhaf149

Wen-Wen Zhang, Feng-Yi Xiao, Cun-Yu Li, Hong-Zhiyuan Yang, Dong Zhao, Jian-Hui Ye, Xin-Qiang Zheng, Yue-Rong Liang, Zhou-Tao Fang, Jian-Liang Lu

{"title":"Functional Analysis of a UDP-Glucosyltransferase Gene Contributing to Biosynthesis of the Flavonol Tri-glycoside in Tea Plants","authors":"Wen-Wen Zhang, Feng-Yi Xiao, Cun-Yu Li, Hong-Zhiyuan Yang, Dong Zhao, Jian-Hui Ye, Xin-Qiang Zheng, Yue-Rong Liang, Zhou-Tao Fang, Jian-Liang Lu","doi":"10.1093/hr/uhaf149","DOIUrl":"https://doi.org/10.1093/hr/uhaf149","url":null,"abstract":"Flavonol glycosides have many prominent benefits to human health and significant contributions to the growth and development of tea plant as well as the color and taste of tea infusion. In this study, a gene has been isolated from tea plant and encodes a 52.2 kDa protein located on the plasma membrane and in cytoplasm with an activity of flavonol glycosyltransferase(CsFGT). The prokaryotically expressed recombinant CsFGT (rCsFGT) exhibits its main glucosyl-transfer activity towards rutin to produce quercetin 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosy-(1→6)-β-D-glucopyranoside(Q-g-r-g), and shows a minor galactosyl-transfer activity towards delphinidin to produce delphinidin 3-O galactoside. The maximum activity of the rCsFGT has been observed at 30 °C and pH 8.0. Main function of the rCsFGT seems to catalyze the biosynthesis of Q-g-r-g rather than delphinidin 3-O galactoside since its affinity and catalytic efficiency are much higher towards rutin than towards delphinidin. The molecular docking and site-directed mutation reveal amino acid residues G290, E292, R319 and Q352 play important roles in catalytic specificity of the CsFGT. The Q-g-r-g content in leaves of different tea cultivars is significantly correlated with the CsFGT expression level. Injection of antisense oligodeoxyribonucleotides remarkably downregulates the endogenous CsFGT expression and consequently reduces the Q-g-r-g content significantly. These findings will help elucidate the differential accumulation mechanism of flavonol glycosides in different tea germplasms.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"1 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptomic analyses reveal cellular and molecular patterns of rose petal responses to gray mold infection 单细胞转录组学分析揭示了玫瑰花瓣对灰霉菌感染反应的细胞和分子模式

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-09 DOI: 10.1093/hr/uhaf152

Xuejiao Li, Yinqi Siman, Yan Zhao, Lvchun Peng, Hongzhi Wu, Wenling Guan, Jingli Zhang, Yanfei Cai, Zhengan Yang, Gengyun Li, Jing Meng, Shuilian He

{"title":"Single-cell transcriptomic analyses reveal cellular and molecular patterns of rose petal responses to gray mold infection","authors":"Xuejiao Li, Yinqi Siman, Yan Zhao, Lvchun Peng, Hongzhi Wu, Wenling Guan, Jingli Zhang, Yanfei Cai, Zhengan Yang, Gengyun Li, Jing Meng, Shuilian He","doi":"10.1093/hr/uhaf152","DOIUrl":"https://doi.org/10.1093/hr/uhaf152","url":null,"abstract":"Roses (Rosa hybrida) are the most popular cut flower plants worldwide, accounting for over a third of the global cut flower industry. Gray mold, caused by Botrytis cinerea, is often referred to as the postharvest \"cancer\" of cut roses and represents the most significant disease impacting the postharvest preservation of these flowers in China. Currently, research progress in this area has been limited. Our study utilized single-cell RNA sequencing (scRNA-seq) technology to elucidate the mechanisms underlying B. cinerea resistance in R. hybrida 'Jumilia'. We identified seven distinct cell groups within rose petals. The rose epidermis acts as the physical barrier of defense against B. cinerea, while the infection rate may be accelerated through vascular tissues. Furthermore, we identified several key genes, including pectin methylesterases (PMEs), pathogenesis-related proteins (PRPs), glutathione S-transferase, and endochitinase EP3, which may play crucial roles in the stress response. The biosynthesis of secondary metabolites temporarily mitigates the infection process, and pathogenesis-related proteins (FRAs) have been recognized as key regulatory genes. This preliminary study elucidates the cellular changes and molecular mechanisms involved in B. cinerea infection in rose petals at the single-cell level. Our findings provide new insights into the defense mechanisms of roses against fungal diseases.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"7 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144252314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GWAS identifies a molecular marker cluster associated with monoterpenoids in grapes GWAS鉴定了葡萄中与单萜类化合物相关的分子标记簇

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-06-06 DOI: 10.1093/hr/uhaf144

Hui-Min Zhang, Xin-Jie Lyu, Zheng-Yang Sun, Qi Sun, Ya-Chen Wang, Lei Sun, Hai-Ying Xu, Lei He, Chang-Qing Duan, Qiu-Hong Pan

{"title":"GWAS identifies a molecular marker cluster associated with monoterpenoids in grapes","authors":"Hui-Min Zhang, Xin-Jie Lyu, Zheng-Yang Sun, Qi Sun, Ya-Chen Wang, Lei Sun, Hai-Ying Xu, Lei He, Chang-Qing Duan, Qiu-Hong Pan","doi":"10.1093/hr/uhaf144","DOIUrl":"https://doi.org/10.1093/hr/uhaf144","url":null,"abstract":"Monoterpenoids are vital compounds that impart a distinctive floral flavor. They exist in both glycosidic and free forms in grapes. The breeding of improved monoterpenoid varieties has consistently been a topic of interest, yet only a limited number of molecular markers have been documented. This study employed a genome-wide association study (GWAS) on an F1 population crossed between a typical muscat variety (‘Muscat of Alexandria’) and a non-aromatic variety (‘Christmas Rose’), conducted over two consecutive years. A total of 4,089 significant single nucleotide polymorphism sites (sigSNPs) and 892 candidate genes associated with monoterpenoids were identified. The sigSNPs corresponding to the glycosidic and total (glycosidic plus free) concentrations of various monoterpenoid compounds exhibited a high similarity. The majority of sigSNPs were located on chromosome 5, indicating the existence of a monoterpenoid-related marker cluster. Sixty-one lead SNPs located within the gene region and stably appearing in two years were selected and verified using a germplasm population. The alleles of the 25 lead SNPs were confirmed to be highly associated with monoterpenoid levels. The genes containing these lead SNPs were mainly glycoside hydrolase, ABC transporter, as well as the previously reported 1-deoxy-D-xylulose-5-phosphate synthase (VvDXS1) and geranylgeranyl pyrophosphate synthase large subunit (VvGGPPS-LSU). The function of VvGGPPS-LSU in regulating monoterpenoid levels was elucidated through in vivo overexpression, demonstrating the reliability of the marker cluster. The present study proposes a molecular marker set for the breeding with the objective of improving aroma, and a candidate gene network for the regulation of monoterpenoid synthesis in grapevine.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"33 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHROMOMETHYLASE3 governs male fertility to affect seed production in tomato CHROMOMETHYLASE3控制番茄雄性育性，影响种子产量

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-05-30 DOI: 10.1093/hr/uhaf143

Huihui Zhu, Weiwei Chen, Zheng’an Yang, Liang Chen, Li Huang, Yiguo Hong, Jianli Yang

{"title":"CHROMOMETHYLASE3 governs male fertility to affect seed production in tomato","authors":"Huihui Zhu, Weiwei Chen, Zheng’an Yang, Liang Chen, Li Huang, Yiguo Hong, Jianli Yang","doi":"10.1093/hr/uhaf143","DOIUrl":"https://doi.org/10.1093/hr/uhaf143","url":null,"abstract":"To produce mature seed, flowering plants must undergo successful male and female gametogenesis and pollination followed by fruit set, growth, and ripening. This sequential process involves complex genetic programming and less understood epigenetic re-programming. Here we report a previously unidentified CHROMOMETHYLASE3-directed epi-control in pollen mother cell (PMC)-to-microspore transition that determines male fertility to affect seed formation. We generated and characterized hairpin RNA-mediated RNAi and CRISPR/Cas9 transgenic tomato lines in which CHROMOMETHYLASE3 (CMT3) was either knocked-down (KD) or knocked-out (KO). CHROMOMETHYLASE3 has pleiotropic effects on vegetative and reproductive growth including leaf, flower and seed development, besides its influence on tomato ripening and fruit size. However, CMT3 KD plants exhibited stronger effects than KO plants in terms of these vegetative and reproductive processes. RT-qPCR analysis suggested that genetic compensation might contribute to the less impacts of KO plants on pollen and seed development. Integrated RNA-seq and whole-genome bisulfite sequencing (WGBS) reveal that CMT3 functions as an epi-switch via a self-feedback mechanism to modulate gene expression and governs early development of microspores from PMCs prior to the tetrad stage during microsporogenesis-to-microgametogenesis, possibly by pectin catabolic process to establish pollen fertility that affects seed production in tomato.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"35 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144176638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0