Horticulture Research最新文献

{"title":"mRNA splicing links surface immunity to NLR activation: implications for horticultural disease resistance","authors":"Jian Meng,Shaoling Zhang,Xiaosan Huang","doi":"10.1093/hr/uhag431","DOIUrl":"https://doi.org/10.1093/hr/uhag431","url":null,"abstract":"","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"26 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reframing Citrus Iron Deficiency Chlorosis as a Whole-Tree Iron Homeostasis Disorder","authors":"Tianchi Jiang, Wan Zhou, Yiming Ma, Patrick Brown, Lingli Lu","doi":"10.1093/hr/uhag436","DOIUrl":"https://doi.org/10.1093/hr/uhag436","url":null,"abstract":"Iron deficiency chlorosis is a recurrent nutritional disorder that limits citrus growth, productivity and fruit quality, especially in calcareous, saline-alkaline and bicarbonate-rich orchards. Although it is often attributed to poor iron bioavailability in the rhizosphere, its persistence and recurrence cannot be explained by soil chemistry or root uptake alone. Citrus trees are perennial evergreen fruit crops with concurrent sinks, including roots, mature leaves, young flushes, flowers and developing fruits; therefore, iron nutrition depends on rootstock genotype, vascular transport, phloem remobilization, leaf-level iron bioavailability and seasonal source-sink allocation. Here, we reframe citrus iron deficiency chlorosis as a systemic disorder of whole-tree iron homeostasis. We synthesize citrus-specific evidence and broader plant iron-homeostasis concepts to connect rhizosphere iron mobilization, Strategy I root responses, rootstock-mediated acquisition, long-distance transport, the leaf iron paradox, reproductive sink allocation, management strategies and emerging multi-omics approaches. We emphasize that total iron concentration alone is insufficient for diagnosis because iron may be spatially misallocated, chemically immobilized or physiologically unavailable even when bulk levels appear adequate. We further propose a bottleneck-oriented diagnostic workflow linking symptom timing, soil and irrigation constraints, active iron status, root acquisition capacity, vascular transport, spatial localization and confounding stresses to targeted management. This framework provides a crop-specific roadmap for identifying dominant homeostasis bottlenecks, improving iron-efficient rootstock selection and designing precision strategies for recurrent citrus chlorosis.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"10 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grafting-associated chilling tolerance in grapevine involves VvERF113-regulated stilbene biosynthesis and changes in chromatin accessibility","authors":"Bin Han, Yanyan Zheng, Jingjing Li, Xuanyu Chen, Minmin Li, Zain Ali, Wenyuan Wang, Yonggang Yin, Shuli Han, Changjiang Liu, Nan Jia, Xinyu Wang, Pingyin Guan, Yan Sun, Yang Liu","doi":"10.1093/hr/uhag433","DOIUrl":"https://doi.org/10.1093/hr/uhag433","url":null,"abstract":"Grapevine (Vitis vinifera L.) is highly sensitive to low temperature, yet the molecular basis by which rootstocks improve scion chilling tolerance remains poorly understood. Here, we show that the cold-tolerant rootstock CR3 markedly enhances chilling tolerance in the grape cultivar ‘Miguang’. Integrated ATAC-seq and RNA-seq analyses revealed that grafted plants underwent broader chilling-induced changes in chromatin accessibility than self-rooted plants, particularly in genomic regions associated with stress responses and secondary metabolism. Among these pathways, stilbene biosynthesis was strongly activated in grafted plants, accompanied by increased expression of VvSTS genes and higher accumulation of stilbene compounds. Exogenous resveratrol treatment further alleviated chilling-induced damage, supporting a protective role for stilbenes in grapevine chilling adaptation. We further identified the ethylene-responsive transcription factor VvERF113 as a direct repressor of VvSTS expression. Overexpression of VvERF113 suppressed stilbene accumulation and reduced chilling tolerance, whereas RNA interference of VvERF113 promoted stilbene biosynthesis and enhanced chilling tolerance. Together, these findings indicate that repression of VvERF113 in grafted plants may contribute to the enhanced activation of the stilbene pathway associated with CR3 grafting, thereby improving grapevine chilling tolerance, and provide a mechanistic framework for enhancing cold resistance through scion-rootstock interactions.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"11 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generating popcorn-like fragrant Chinese cabbage via CRISPR/Cas9-mediated\u0000 BraBADH2\u0000 gene editing","authors":"Yunxiao Wei,Xiaojing Zeng,Lingyan Xu,Fei Li,Hui Zhang,Qiannan Zhang,Shifan Zhang,Rifei Sun,Rui Zhang,Jianjun Zhao,Shujiang Zhang,Guoliang Li","doi":"10.1093/hr/uhag437","DOIUrl":"https://doi.org/10.1093/hr/uhag437","url":null,"abstract":"","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"14 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population structure and genetic mapping of important agronomic traits in\u0000 Perilla frutescens","authors":"Xu Li,Duan Wu,Wei Zhang,He Wen,Zhiwei Shang,Qi Shen","doi":"10.1093/hr/uhag413","DOIUrl":"https://doi.org/10.1093/hr/uhag413","url":null,"abstract":"","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"11 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potassium sulfate modulates FabHLH18-like expression by a dual mechanism to regulate sugar and anthocyanin accumulation in strawberries","authors":"Zihui Zhang, Shanna Yi, Wenjie Yu, Yan Wang, Zhihong Zhang, He Li","doi":"10.1093/hr/uhag439","DOIUrl":"https://doi.org/10.1093/hr/uhag439","url":null,"abstract":"Sugar and anthocyanin contents are key indicators of both the intrinsic and extrinsic qualities of fruits. Application of potassium fertilizers can significantly improve fruit quality in crops; however, the specific molecular regulatory mechanisms underlying this effect remain unclear. In this study, treatment with potassium sulfate significantly improved strawberry fruit quality, while FabHLH18-like was significantly upregulated. Stable and transient transformation of FabHLH18-like in cultivated strawberry ‘Yanli’ both demonstrated that it can increase the sugar and anthocyanin contents. Meanwhile, transient transformation of FaINV and FaANS in cultivated strawberry ‘Yanli’ demonstrated that FaINV can convert sucrose into glucose and fructose, while FaANS can increase anthocyanin synthesis in fruits. Furthermore, the FabHLH18-like protein binds to the promoters of FaINV and FaANS, thereby promoting their expression. FaMYB6-like induced FaHLH18-like-mediated activation of FaINV to increase sugar contents in strawberry fruits; FaWRKY57 and FabHLH18-like synergistically regulated FaANS expression to jointly promote anthocyanin accumulation in strawberry fruits. Furthermore, under potassium sulfate treatment, FaChip expression decreased, weakening its effect in ubiquitinating and degrading FabHLH18-like; simultaneously, the potassium transporter FaPOT3 was continuously transported into fruits following potassium sulfate treatment, thereby enhancing the promotional effect of FabHLH18-like on sugar and anthocyanin contents in strawberry fruits. Our findings are the first to reveal the regulatory pathway through which potassium fertilizers improve strawberry fruit quality by inducing the upregulation of FabHLH18-like, providing molecular targets for enhancing crop fruit quality while offering a theoretical and scientific basis for using potassium fertilizers.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"48 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a maternal haploid induction system in petunia via CRISPR/Cas9-mediated editing of\u0000 PhDMP8/9","authors":"Guoqi Xu,Yu Ma,Lanyu Cao,Jiaojiao Wang,Chenxin Li,Yuxin Yan,Yingjia Zeng,Jiafa Wang,Shujuan Tian,Li Yuan,Man Liu","doi":"10.1093/hr/uhag414","DOIUrl":"https://doi.org/10.1093/hr/uhag414","url":null,"abstract":"","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"236 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Origin and evolution of steroidal saponin biosynthesis and its regulation by the DcWRKY33-DcWRKY11 module in Dioscorea composita","authors":"Shangjie Yu, Peifan Lin, Yinxing Cao, Jiani Zhang, Jun Xie, Lingfei Li, Chunmei Zhong","doi":"10.1093/hr/uhag429","DOIUrl":"https://doi.org/10.1093/hr/uhag429","url":null,"abstract":"Although steroidal saponins from Dioscorea species have substantial economic value and versatile utilization potential, the evolutionary origin and regulatory mechanisms underlying their biosynthesis remain poorly understood. Here, we conducted a comparative genomic analysis across representative plant lineages ranging from algae to angiosperms to examine the copy number variation and syntenic organization of steroidal saponin biosynthetic genes. Our results reveal that ancestral sterol biosynthetic components were already present in bryophytes. However, synteny analysis indicates that stable genomic integration of these genes was established no earlier than in pteridophytes. By integrating transcriptome–metabolome association analyses with branch-site selection tests, we identified strong positive selection acting on DcWRKY33 within the Dioscorea lineage, suggesting adaptive evolution and highlighting the importance of regulatory innovation in steroidal saponin diversification. Functional analyses demonstrated that DcWRKY33 functions as a positive regulator of steroidal saponin biosynthesis: transient overexpression upregulates the expression of structural genes and increases saponin accumulation, whereas gene silencing produced opposite effects. Mechanistically, dual-luciferase and yeast one-hybrid assays indicated that DcWRKY33 activates DcHDR and DcCYP51, while indirectly regulating DcHMGR through modulation of DcWRKY11, thereby orchestrating biosynthesis across both the MEP and MVA pathways. Promoter analysis revealed W-box motifs partially absence in non-saponin-producing Dioscorea, and heterologous expression in tobacco confirmed lineage-specific regulatory capacity despite conserved cis-elements. Collectively, our study links pathway evolution with regulatory innovation and establishes a mechanistic framework for understanding the evolution and transcriptional control of steroidal saponin biosynthesis in D. composita, providing insights for metabolic improvement of medicinal and horticultural yam species.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"98 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CCCH zinc finger protein PtrTZF10 enhances cold tolerance in association with β-amylase-mediated starch degradation and sugar accumulation in Poncirus trifoliata","authors":"Min Wang, Huan Zhong, Zhuoxiang Zhao, Lei Ji, Yue Wu, Yue Ma, Shuqi Ye, Ji-Hong Liu, Wen-Shan Dai","doi":"10.1093/hr/uhag434","DOIUrl":"https://doi.org/10.1093/hr/uhag434","url":null,"abstract":"Cold stress is a major environmental constraint limiting the geographical distribution and productivity of citrus. Poncirus trifoliata, a close cold-hardy relative of citrus, serves as an invaluable reservoir of stress-resistance genes. Here, we functionally characterized PtrTZF10, a cold-responsive CCCH tandem zinc finger gene from P. trifoliata, and demonstrated its positive role in regulating cold tolerance and its association with sugar metabolism. In contrast to the transient induction observed in cold-sensitive lemon, PtrTZF10 was rapidly and persistently induced by cold in P. trifoliata. PtrTZF10 localizes to both the nucleus and plasma membrane, binds the tested DNA and RNA substrates in vitro, and possesses transcriptional activation activity. Ectopic expression of PtrTZF10 in tobacco and lemon significantly enhanced cold tolerance, as reflected by improved photosynthetic efficiency, reduced membrane damage, and lower reactive oxygen species (ROS) accumulation. Conversely, silencing PtrTZF10 in P. trifoliata compromised its freezing tolerance. Transcriptomic profiling under normal growth conditions revealed basal differences in the expression of carbohydrate metabolism-related genes in PtrTZF10-overexpressing lines. Molecular assays further showed that PtrTZF10 associates with and activates the PtrBMY1 promoter, while PtrTZF10 overexpression and silencing were accompanied by corresponding changes in β-amylase activity, starch degradation, and soluble sugar accumulation. Collectively, these findings identify PtrTZF10 as a positive regulator of cold tolerance and support a PtrTZF10-PtrBMY1 regulatory link associated with starch-to-sugar mobilization during the cold response.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"188 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3β-hydroxysteroid-dehydrogenase/decarboxylase 2 ( HSD2 ) is essential for fruit cuticle formation in tomato","authors":"Fangyan Zheng, Long Cui, Feng Hu, Miao Liu, Lesong Jia, Tao Li, Qingmin Xie, Changxian Yang","doi":"10.1093/hr/uhag438","DOIUrl":"https://doi.org/10.1093/hr/uhag438","url":null,"abstract":"The plant cuticle is a multilayered structure composed of cutin, cutin-linked polysaccharides and diverse waxes. It covers aerial plant tissues to prevent water loss and pathogen invasion, and modulates key crop quality traits. Despite substantial progress in research on cuticle formation, its molecular regulatory mechanisms remain largely elusive. In this study, we functionally characterized a tomato mutant exhibiting severe fruit suberization, named CM (cuticle mutation). Genetic analysis revealed that the mutant phenotype is governed by a single recessive gene. Using bulked segregant analysis (BSA), we mapped the causal locus to SNP45564049 in the fourth exon of SlHSD2, a G-to-A substitution producing a glycine-to-serine change. CRISPR/Cas9-mediated knockout of SlHSD2 successfully reproduced the CM mutant phenotype and altered the profile of pericarp cutin monomers. Integrated transcriptomic and widely targeted metabolomic analyses showed that SlHSD2 mutation reshapes the expression of cutin, suberin and wax biosynthetic genes, perturbs fatty acid metabolism and the accumulation of core cutin monomers, and alters numerous metabolites including alkaloids, flavonoids, lipids and phenolic acids, ultimately impairing cuticle development. We further found that the SBP-box transcription factor SlCNR acts as a negative regulator of fruit cutin deposition, directly binding the GTAC motifs in the SlHSD2 promoter to represses its expression. In summary, SlHSD2 is a newly identified downstream target of SlCNR that is essential for tomato fruit cuticle development via the regulation of lipid metabolism. This study expands the regulatory network of fruit cuticle formation and provides valuable gene resources for crop genetic improvement and stress-resistant breeding.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"13 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}