Horticulture Research最新文献

Graft incompatibility between pepper and tomato elicits an immune response and triggers localized cell death 辣椒和番茄之间的嫁接不亲和会引起免疫反应，并引发局部细胞死亡

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-11 DOI: 10.1093/hr/uhae255

Hannah Rae Thomas, Alice Gevorgyan, Alexandra Hermanson, Samantha Yanders, Lindsay Erndwein, Matthew Norman-Ariztía, Erin E Sparks, Margaret H Frank

{"title":"Graft incompatibility between pepper and tomato elicits an immune response and triggers localized cell death","authors":"Hannah Rae Thomas, Alice Gevorgyan, Alexandra Hermanson, Samantha Yanders, Lindsay Erndwein, Matthew Norman-Ariztía, Erin E Sparks, Margaret H Frank","doi":"10.1093/hr/uhae255","DOIUrl":"https://doi.org/10.1093/hr/uhae255","url":null,"abstract":"Graft compatibility is the capacity of two plants to form cohesive vascular connections. Tomato and pepper are incompatible graft partners; however, the underlying cause of graft rejection between these two species remains unknown. We diagnosed graft incompatibility between tomato and diverse pepper varieties based on weakened biophysical stability, decreased growth, and persistent cell death using viability stains. Transcriptomic analysis of the junction was performed using RNA-sequencing, and molecular signatures for incompatible graft response were characterized based on meta-transcriptomic comparisons with other biotic processes. We show that tomato is broadly incompatible with diverse pepper cultivars. These incompatible graft partners activate prolonged transcriptional changes that are highly enriched for defense processes. Amongst these processes was broad nucleotide-binding and leucine-rich repeat receptors (NLR) upregulation and genetic signatures indicative of an immune response. Using transcriptomic datasets for a variety of biotic stress treatments, we identified a significant overlap in the genetic profile of incompatible grafting and plant parasitism. In addition, we found over 1000 genes that are uniquely upregulated in incompatible grafts. Based on NLR overactivity, DNA damage, and prolonged cell death we hypothesize that tomato and pepper graft incompatibility is characterized by an immune response that triggers cell death which interferes with junction formation.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Converging functional phenotyping with systems mapping to illuminate the genotype–phenotype associations 将功能表型与系统图谱相结合，阐明基因型与表型之间的联系

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-09 DOI: 10.1093/hr/uhae256

Ting Sun, Zheng Shi, Rujia Jiang, Menachem Moshelion, Pei Xu

{"title":"Converging functional phenotyping with systems mapping to illuminate the genotype–phenotype associations","authors":"Ting Sun, Zheng Shi, Rujia Jiang, Menachem Moshelion, Pei Xu","doi":"10.1093/hr/uhae256","DOIUrl":"https://doi.org/10.1093/hr/uhae256","url":null,"abstract":"Illuminating the phenotype–genotype black box under complex traits is an ambitious goal for researchers. The generation of temporally or spatially phenotypic data today has far outpaced its interpretation, due to their highly dynamic nature depending on the environment and developmental stages. Here we propose an integrated enviro-pheno-geno functional approach to pinpoint the major challenges of decomposing physiological traits. The strategy first features high-throughput functional physiological phenotyping (FPP) to efficiently acquire phenotypic and environmental data. It then features functional mapping (FM) and the extended systems mapping (SM) to tackle trait dynamics. FM, by modeling traits as continuous functions, can increase the power and efficiency in dissecting the spatiotemporal effects of QTLs. SM could enable reconstruction of a genotype–phenotype map from developmental pathways. We present a recent case study that combines FPP and SM to dissect complex physiological traits. This integrated approach will be an important engine to drive the translation of phenomic big data into genetic gain.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142160466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potassium stimulates fruit sugar accumulation by increasing carbon flow in Citrus sinensis 钾通过增加碳流刺激柑橘果实糖分积累

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-09 DOI: 10.1093/hr/uhae240

Kongjie Wu, Chengxiao Hu, Peiyu Liao, Yinlong Hu, Xuecheng Sun, Qiling Tan, Zhiyong Pan, Shoujun Xu, Zhihao Dong, Songwei Wu

{"title":"Potassium stimulates fruit sugar accumulation by increasing carbon flow in Citrus sinensis","authors":"Kongjie Wu, Chengxiao Hu, Peiyu Liao, Yinlong Hu, Xuecheng Sun, Qiling Tan, Zhiyong Pan, Shoujun Xu, Zhihao Dong, Songwei Wu","doi":"10.1093/hr/uhae240","DOIUrl":"https://doi.org/10.1093/hr/uhae240","url":null,"abstract":"Soluble sugars contribute to the taste and flavor of citrus fruit. Potassium (K), known as a quality element, plays key roles in improving sugar accumulation and fruit quality, but the mechanism is largely unknown. This study aims to elucidate how K improves sugar accumulation by regulating carbon flow from source leaves to fruit in Newhall navel orange. We found that optimal fruit K concentrations around 1.5% improved sugar accumulation and fruit quality in citrus. K application increased the strength of both sink and source, as indicated by the increased fruit growth rate, enzymes activities and expression levels of key genes involved in sucrose (Suc) metabolism in fruit and leaf. K application also facilitated Suc transport from source leaves to fruit, as confirmed by the enhanced 13C-Suc level in fruit. Furthermore, we found that navel orange used symplastic pathway for transporting Suc from source leaves to fruits, and K application enhanced symplastic loading, as demonstrated by the intensified CF signal and increased plasmodesmata density in leaves. The findings reveal that K stimulates fruit sugar accumulation by increasing carbon flow from source leaves to fruit in Newhall navel orange.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142160802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-resolution genetic map and SNP chip for molecular breeding in Panax ginseng, a tetraploid medicinal plant 用于四倍体药用植物人参分子育种的高分辨率遗传图谱和 SNP 芯片

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-09 DOI: 10.1093/hr/uhae257

Woohyeon Cho, Woojong Jang, Hyeonah Shim, Jiseok Kim, Youngju Oh, Jee Young Park, Young Chang Kim, Jung-Woo Lee, Ick-Hyun Jo, Misun Lee, Jinsu Gil, Martin Mascher, Murukarthick Jayakodi, Xuejiao Liao, Jiang Xu, Deqiang Dou, Yi Lee, Tae-Jin Yang

{"title":"High-resolution genetic map and SNP chip for molecular breeding in Panax ginseng, a tetraploid medicinal plant","authors":"Woohyeon Cho, Woojong Jang, Hyeonah Shim, Jiseok Kim, Youngju Oh, Jee Young Park, Young Chang Kim, Jung-Woo Lee, Ick-Hyun Jo, Misun Lee, Jinsu Gil, Martin Mascher, Murukarthick Jayakodi, Xuejiao Liao, Jiang Xu, Deqiang Dou, Yi Lee, Tae-Jin Yang","doi":"10.1093/hr/uhae257","DOIUrl":"https://doi.org/10.1093/hr/uhae257","url":null,"abstract":"Ginseng (Panax ginseng) renowned as the king of medicinal plants. Ginseng grows slowly under shade conditions, requiring at least four years to produce a limited number of seeds. Molecular breeding of ginseng faces challenges due to its the tetraploid genome and the absence of an efficient molecular marker system. To overcome these obstacles, we adopted genotyping-by-sequencing to delve into genetic mapping and survey genetic diversity. We constructed a comprehensive genetic map comprising 24 linkage groups, each corresponding to one of the 24 chromosomes in the ginseng genome, based on 1216 non-redundant SNPs obtained from an F2 mapping population. Additionally, 431 103 SNPs were identified from 119 diverse ginseng genotypes. From these, 192 informative subgenome-specific single copy SNPs were selected to develop a SNP chip. The SNP chip was used to genotype a large ginseng collection, encompassing registered cultivars, breeding lines, wild-simulated ginseng, and wild ginseng from various countries and regions. We evaluated the utility of the assay for molecular breeding with 919 ginseng genotypes. This breeder-friendly SNP chip promises versatility, enabling purity assessments of seeds and products, the authentication of species and cultivars, and the determination of homozygosity and homogeneity rates for breeding lines. Genotype data for 1200 ginseng genotypes are now stored in our database. This SNP chip lays the foundation for a molecular breeding in ginseng and will facilitate the breeding process in this medicinal crop.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142160468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring genetic diversity, population structure, and subgenome differences in the allopolyploid Camelina sativa: implications for future breeding and research studies 探索全多倍体荠菜的遗传多样性、种群结构和亚基因组差异：对未来育种和研究的影响

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-09 DOI: 10.1093/hr/uhae247

Jordan R Brock, Kevin A Bird, Adrian E Platts, Fabio Gomez-Cano, Suresh Kumar Gupta, Kyle Palos, Caylyn E Railey, Scott J Teresi, Yun Sun Lee, Maria Magallanes-Lundback, Emily G Pawlowski, Andrew D L Nelson, Erich Grotewold, Patrick P Edger

{"title":"Exploring genetic diversity, population structure, and subgenome differences in the allopolyploid Camelina sativa: implications for future breeding and research studies","authors":"Jordan R Brock, Kevin A Bird, Adrian E Platts, Fabio Gomez-Cano, Suresh Kumar Gupta, Kyle Palos, Caylyn E Railey, Scott J Teresi, Yun Sun Lee, Maria Magallanes-Lundback, Emily G Pawlowski, Andrew D L Nelson, Erich Grotewold, Patrick P Edger","doi":"10.1093/hr/uhae247","DOIUrl":"https://doi.org/10.1093/hr/uhae247","url":null,"abstract":"Camelina (Camelina sativa), an allohexaploid species, is an emerging aviation biofuel crop that has been the focus of resurgent interest in recent decades. To guide future breeding and crop improvement efforts, the community requires a deeper comprehension of subgenome dominance, often noted in allopolyploid species, alongside and understanding of the genetic diversity and population structure of material present within breeding programs. We conducted population genetic analyses of a C. sativa diversity panel, leveraging a new genome, to estimate nucleotide diversity and population structure, and analyzed for patterns of subgenome expression dominance among different organs. Our analyses confirm that C. sativa has relatively low genetic diversity, and show that the SG3 subgenome has substantially lower genetic diversity compared to the other two subgenomes. Despite the low genetic diversity, our analyses identified thirteen distinct subpopulations including two distinct wild populations and others putatively representing founders in existing breeding populations. When analyzing for subgenome composition of long non-coding RNAs, which are known to play important roles in (a)biotic stress tolerance, we found that the SG3 subgenome contained significantly more lincRNAs compared to other subgenomes. Similarly, transcriptome analyses revealed that expression dominance of SG3 is not as strong as previously reported, and may not be universal across all organ-types. From a global analysis, SG3 was only significant higher expressed in flower, flower bud, and fruit organs, which is an important discovery given that the crop yield is associated with these organs. Collectively, these results will be valuable for guiding future breeding efforts in camelina.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142160482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haplotype-resolved nonaploid genome provides insights into in vitro flowering in bamboo 单倍型解析的非单倍体基因组为了解竹子离体开花提供了线索

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-05 DOI: 10.1093/hr/uhae250

Yu-Jiao Wang, Cen Guo, Zhao Lei, Ling Mao, Xiang-Zhou Hu, Yi-Zhou Yang, Ke-Cheng Qian, Peng-Fei Ma, Zhen-Hua Guo, De-Zhu Li

{"title":"Haplotype-resolved nonaploid genome provides insights into in vitro flowering in bamboo","authors":"Yu-Jiao Wang, Cen Guo, Zhao Lei, Ling Mao, Xiang-Zhou Hu, Yi-Zhou Yang, Ke-Cheng Qian, Peng-Fei Ma, Zhen-Hua Guo, De-Zhu Li","doi":"10.1093/hr/uhae250","DOIUrl":"https://doi.org/10.1093/hr/uhae250","url":null,"abstract":"Woody bamboo (Bambusoideae) is renowned for its polyploidy and rare flowering. Bambusa odashimae is one of the bamboo species with the highest chromosome count (104) in the subfamily and has the highest heterozygosity of all sequenced bamboo genomes so far. Compared with other bamboo species, it can efficiently utilize exogenous hormones to regulate in vitro flowering, providing valuable insights into the hormonal regulation of bamboo flowering. Here, we generated the haplotype-resolved genome assembly of B. odashimae, despite the complexity and high chromosome number, supplemented by thirty-three transcriptomes from eleven developmental periods using a tissue culture system. The assembled genome can be divided into Haplotype I, Haplotype II, and Haplotype III, each containing A, B, and C subgenomes. Haplotype I may be derived from Dendrocalamus whereas Haplotypes II and III are closely related to Bambusa, indicating that B. odashimae has an origin involving both intergeneric and interspecific hybridizations. The high heterozygosity renders the possibility to detect abundant allele-specific expression (ASE), with ASE genes enriched in cytokinin-related pathways, likely associated with efficient cytokinin-promoted flowering. Notably, we found that the CONSTANS (CO) genes were potentially key regulators of in vitro flowering in B. odashimae. Overall, our study, based on the in vitro system combined with a high-quality reference genome, provides critical insights into the origin of this nonaploid bamboo and links hybridization and in vitro flowering in bamboo.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A low-cost dpMIG-seq method for elucidating complex inheritance in polysomic crops: A case study in tetraploid blueberry 用于阐明多倍体作物复杂遗传的低成本 dpMIG-seq 方法：四倍体蓝莓的案例研究

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-05 DOI: 10.1093/hr/uhae248

Kyoka Nagasaka, Kazusa Nishimura, Ko Motoki, Keigo Yamagata, Soichiro Nishiyama, Hisayo Yamane, Ryutaro Tao, Ryohei Nakano, Tetsuya Nakazaki

{"title":"A low-cost dpMIG-seq method for elucidating complex inheritance in polysomic crops: A case study in tetraploid blueberry","authors":"Kyoka Nagasaka, Kazusa Nishimura, Ko Motoki, Keigo Yamagata, Soichiro Nishiyama, Hisayo Yamane, Ryutaro Tao, Ryohei Nakano, Tetsuya Nakazaki","doi":"10.1093/hr/uhae248","DOIUrl":"https://doi.org/10.1093/hr/uhae248","url":null,"abstract":"Next-generation sequencing (NGS) library construction often requires high-quality DNA extraction, precise adjustment of DNA concentration, and restriction enzyme digestion to reduce genome complexity, which results in increased time and cost in sample preparation and processing. To address these challenges, a PCR-based method for rapid NGS library preparation, named dpMIG-seq, has been developed and proven effective for high-throughput genotyping. However, the application of dpMIG-seq has been limited to diploid and polyploid species with disomic inheritance. In this study, we obtained genome-wide single nucleotide polymorphism (SNP) markers for tetraploid blueberry to evaluate genotyping and downstream analysis outcomes. Comparison of genotyping qualities inferred across samples with different DNA concentrations and multiple bioinformatics approaches revealed high accuracy and reproducibility of dpMIG-seq-based genotyping, with Pearson’s correlation coefficients between replicates in the range of 0.91 to 0.98. Furthermore, we demonstrated that dpMIG-seq enables accurate genotyping of samples with low DNA concentrations. Subsequently, we applied dpMIG-seq to a tetraploid F1 population to examine the inheritance probability of parental alleles. Pairing configuration analysis supported the random meiotic pairing of homologous chromosomes on a genome-wide level. On the other hand, preferential pairing was observed on chr-11, suggesting that there may be an exception to the random pairing. Genotypic data suggested quadrivalent formation within the population, although the frequency of quadrivalent formation varied by chromosome and cultivar. Collectively, the results confirmed applicability of dpMIG-seq for allele dosage genotyping and are expected to catalyze the adoption of this cost-effective and rapid genotyping technology in polyploid studies.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De novo gene integration into regulation networks via interaction with conserved genes in peach 通过与桃中保守基因的相互作用将新基因整合到调控网络中

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-05 DOI: 10.1093/hr/uhae252

Yunpeng Cao, Jiayi Hong, Yun Zhao, Xiaoxu Li, Xiaofeng Feng, Han Wang, Lin Zhang, Mengfei Lin, Yongping Cai, Yuepeng Han

{"title":"De novo gene integration into regulation networks via interaction with conserved genes in peach","authors":"Yunpeng Cao, Jiayi Hong, Yun Zhao, Xiaoxu Li, Xiaofeng Feng, Han Wang, Lin Zhang, Mengfei Lin, Yongping Cai, Yuepeng Han","doi":"10.1093/hr/uhae252","DOIUrl":"https://doi.org/10.1093/hr/uhae252","url":null,"abstract":"De novo genes can evolve 'from scratch' from non-coding sequences, acquiring novel functions in organisms and integrating into regulatory networks during evolution to drive innovations in important phenotypes and traits. However, identifying de novo genes is challenging, as it requires high-quality genomes from closely related species. According to the comparison with nine closely related Prunus genomes, we determined at least 178 de novo genes in P. persica ‘baifeng’. The distinct differences were observed between de novo and conserved genes in gene characteristics and expression patterns. Gene ontology (GO) enrichment analysis suggested that Type I de novo genes originated from sequences related to plastid modification functions, while Type II genes were inferred to have derived from sequences related to reproductive functions. Finally, transcriptome sequencing across different tissues and developmental stages suggested that de novo genes have been evolutionarily recruited into existing regulatory networks, playing important roles in plant growth and development, which was also supported by WGCNA analysis and quantitative trait loci data. This study lays the groundwork for future research on the origins and functions of genes in Prunus and related taxa.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TOR balances plant growth and cold tolerance by orchestrating amino acid-derived metabolism in tomato TOR 通过协调番茄的氨基酸代谢平衡植物生长和耐寒性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-03 DOI: 10.1093/hr/uhae253

Zihao Li, Lin Yang, Yanni Wu, Ran Zhang, Sen Yu, Liwen Fu

{"title":"TOR balances plant growth and cold tolerance by orchestrating amino acid-derived metabolism in tomato","authors":"Zihao Li, Lin Yang, Yanni Wu, Ran Zhang, Sen Yu, Liwen Fu","doi":"10.1093/hr/uhae253","DOIUrl":"https://doi.org/10.1093/hr/uhae253","url":null,"abstract":"The target of rapamycin (TOR) kinase is a central signaling hub that plays a crucial role in precisely orchestrating plant growth, development, and stress responses. This suggests that TOR is intricately involved in maintaining the balance between plant growth and stress responses. Nevertheless, despite the observed effects, the specific mechanisms through which TOR operates in these processes remain obscure. In this study, we investigated how the tomato (Solanum lycopersicum) TOR (SlTOR) affects plant growth and cold responses. We demonstrated that SlTOR inhibition transcriptionally primes cold stress responses, consequently enhancing tomato cold tolerance. A widely targeted metabolomics analysis revealed the disruption of amino acid metabolism homeostasis under cold stress upon SlTOR inhibition, which led to the accumulation of two important cryoprotective metabolites: salicylic acid (SA) and putrescine (Put). Next, we discovered SlPGH1 (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) as a direct substrate of SlTOR. Inhibiting SlTOR led to increased SlCBF1 (C-REPEAT-BINDING FACTOR 1) expression via SlPGH1, potentially triggering the activation of cold-responsive genes and subsequent metabolic alterations. Our study provides a mechanistic framework that elucidates how SlTOR modulates amino acid-related metabolism to enhance tomato cold tolerance, which sheds light on the complex interplay between growth and stress responses orchestrated by TOR.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apple vacuolar sugar transporters regulated by MdDREB2A enhance drought resistance by promoting accumulation of soluble sugars and activating ABA signaling 受 MdDREB2A 调控的苹果液泡糖转运体通过促进可溶性糖积累和激活 ABA 信号转导增强抗旱性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-09-03 DOI: 10.1093/hr/uhae251

Lingcheng Zhu, Chunxia Zhang, Nanxiang Yang, Wenjing Cao, Yanzhen Li, Yunjing Peng, Xiaoyu Wei, Baiquan Ma, Fengwang Ma, Yong-Ling Ruan, Mingjun Li

{"title":"Apple vacuolar sugar transporters regulated by MdDREB2A enhance drought resistance by promoting accumulation of soluble sugars and activating ABA signaling","authors":"Lingcheng Zhu, Chunxia Zhang, Nanxiang Yang, Wenjing Cao, Yanzhen Li, Yunjing Peng, Xiaoyu Wei, Baiquan Ma, Fengwang Ma, Yong-Ling Ruan, Mingjun Li","doi":"10.1093/hr/uhae251","DOIUrl":"https://doi.org/10.1093/hr/uhae251","url":null,"abstract":"Soluble sugars are not only an important contributor to fruit quality, but also serve as the osmotic regulators in response to abiotic stresses. Early drought stress promotes sugar accumulation, while specific sugar transporters govern the cellular distribution of the sugars. Here, we show that apple plantlets accumulate soluble sugars in leaf tissues under drought stress. Transcriptional profiling of stressed and control plantlets revealed differential expression of several plasma membrane- or vacuolar membrane-localized sugar transporter genes. Among these, four previously identified vacuolar sugar transporter (VST) genes (MdERDL6–1, MdERDL6–2, MdTST1 and MdTST2) showed higher expression under drought, suggesting their roles in response to drought stress. Promoter cis-elements analyses, yeast one-hybrid and dual-luciferase tests confirmed that the drought-induced transcription factor MdDREB2A could promote the expression of MdERDL6–1/−2 and MdTST1/2 by binding to their promoter regions. Moreover, overexpressing of each of these four MdVSTs alone in transgenic apple or Arabidopsis plants accumulated more soluble sugars and abscisic acid, and enhanced drought resistance. Furthermore, apple plants overexpressing MdERDL6–1 also showed reduced water potential, facilitated stomatal closure and reactive oxygen species scavenging under drought condition compared to control plants. Overall, our results suggest a potential strategy to enhance drought resistance and sugar accumulation in fruits through manipulating the genes involved in vacuolar sugar transport.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":null,"pages":null},"PeriodicalIF":8.7,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0