Horticulture Research最新文献

Domestication history and genetic changes for the newly evolved flower color in the ornamental plant Lobularia maritima (Brassiaceae)

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-19 DOI: 10.1093/hr/uhae355

Wenjie Yang, Meng Liu, Landi Feng, Pengfei Jiao, Jiebei Jiang, Li Huang, Jianquan Liu, Jordi Lopez-Pujol, Quanjun Hu

{"title":"Domestication history and genetic changes for the newly evolved flower color in the ornamental plant Lobularia maritima (Brassiaceae)","authors":"Wenjie Yang, Meng Liu, Landi Feng, Pengfei Jiao, Jiebei Jiang, Li Huang, Jianquan Liu, Jordi Lopez-Pujol, Quanjun Hu","doi":"10.1093/hr/uhae355","DOIUrl":"https://doi.org/10.1093/hr/uhae355","url":null,"abstract":"Lobularia maritima (sweet alyssum) is a popular ornamental plant that displays a range of flower colors, particularly white and purple. However, the genetic underpinning and evolutionary history of flower colors have remained unknown. To address this, we performed a de novo assembly of a chromosome-level genome for this species and conducted comparative population genomic analyses of both domestic and wild representatives. These analyses revealed distinct genetic clusters corresponding to wild and domestic groups, with further subdivisions based on geographic and phenotypic differences. Importantly, all cultivars originated from a single domestication event within the Tunisia group. One wild group did not contribute genetically to the current cultivars. The new mutations in key gene of the anthocyanin biosynthetic pathway, PAP1, that arose following domestication led to the origin of purple flower coloration in the cultivars. Moreover, the contrasting PAP1 haplotypes in white and purple varieties lead to differential expression of CHS and DFR, which in turn contributes to the observed flower color differences. These findings provide key insights into the domestication history and genetic regulation of flower color in L. maritima, laying the groundwork for future genetic breeding efforts focused on this plant, especially introducing genetic sources from other wild groups.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"14 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142858435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The AREB transcription factor SaAREB6 promotes drought stress-induced santalol biosynthesis in sandalwood

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-17 DOI: 10.1093/hr/uhae347

Sen Meng, Na Lian, Fangcuo Qin, Shuqi Yang, Dong Meng, Zhan Bian, Li Xiang, Junkun Lu

{"title":"The AREB transcription factor SaAREB6 promotes drought stress-induced santalol biosynthesis in sandalwood","authors":"Sen Meng, Na Lian, Fangcuo Qin, Shuqi Yang, Dong Meng, Zhan Bian, Li Xiang, Junkun Lu","doi":"10.1093/hr/uhae347","DOIUrl":"https://doi.org/10.1093/hr/uhae347","url":null,"abstract":"Sandalwood (Santalum album), a culturally significant and economically valuable horticultural species, is renowned for its heartwood and essential oils enriched with sesquiterpene compounds such as santalol. Despite progress in elucidating the biosynthetic pathway of these valuable metabolites, the transcriptional regulation of this process, particularly under abiotic stress conditions, remains largely unexplored. Under drought conditions, we observed a marked increase in SaAREB6 expression, paralleled by elevated levels of santalols. Moreover, we identified SaCYP736A167, a cytochrome P450 monooxygenase gene, as a direct target of SaAREB6. Using electrophoretic mobility shift assays (EMSAs), microscale thermophoresis assays (MSTs) and dual luciferase assays (DLAs), we validated the precise and specific interaction of SaAREB6 with the promoter region of SaCYP736A167. This interaction leads to the upregulation of SaCYP736A167, which in turn catalyzes the final steps in the conversion of sesquiterpene precursors to santalols, thereby reinforcing the connection between SaAREB6 activity and increased santalol production during drought. Collectively, our work illuminates the previously uncharacterized role of SaAREB6 in orchestrating a transcriptional regulation that facilitates drought-induced santalol biosynthesis in sandalwood, presenting opportunities for genetic engineering strategies to improve heartwood and essential oil yields in this economically vital species.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"256 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Resolution Genome Assembly and Population Genetic Study of the Endangered Maple Acer pentaphyllum (Sapindaceae): Implications for Conservation Strategies

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-17 DOI: 10.1093/hr/uhae357

Xiong Li, Li-Sha Jiang, Heng-Ning Deng, Qi Yu, Wen-Bin Ju, Xiao-Juan Chen, Yu Feng, Bo Xu

{"title":"High-Resolution Genome Assembly and Population Genetic Study of the Endangered Maple Acer pentaphyllum (Sapindaceae): Implications for Conservation Strategies","authors":"Xiong Li, Li-Sha Jiang, Heng-Ning Deng, Qi Yu, Wen-Bin Ju, Xiao-Juan Chen, Yu Feng, Bo Xu","doi":"10.1093/hr/uhae357","DOIUrl":"https://doi.org/10.1093/hr/uhae357","url":null,"abstract":"Acer pentaphyllum Diels (Sapindaceae), a highly threatened maple endemic to the dry-hot valleys of the Yalong River in western Sichuan, China, represents a valuable resource for horticulture and conservation. This study presents the first chromosomal-scale genome assembly of A. pentaphyllum (~626 Mb, 2n = 26), constructed using PacBio HiFi and Hi-C sequencing technologies. Comparative genomic analyses revealed significant recent genomic changes through rapid amplification of transposable elements, particularly long terminal repeat retrotransposons, coinciding with the dramatic climate change during recent uplift of the Hengduan Mountains. Genes involved in photosynthesis, plant hormone signal transduction, and plant-pathogen interaction showed expansion and/or positive selection, potentially reflecting adaptation to the species’ unique dry-hot valley habitat. Population genomic analysis of 227 individuals from 28 populations revealed low genetic diversity (1.04 ± 0.97 × 10-3) compared to other woody species. Phylogeographic patterns suggest an unexpected upstream colonization along the Yalong River, while Quaternary climate fluctuations drove its continuous lineage diversification and population contraction. Assessment of genetic diversity, inbreeding, and genetic load across populations revealed concerning levels of inbreeding and accumulation of deleterious mutations in small, isolated populations, particularly those at range edges (TKX, CDG, TES). Based on these results, we propose conservation strategies, including the identification of management units and recommendations for genetic rescue. These findings not only facilitate the conservation of A. pentaphyllum but also serve as a valuable resource for future horticultural development and as a model for similar studies on other endangered plant species adapted to extreme environments.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"697 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Key genes in a “Galloylation-Degalloylation cycle” controlling the synthesis of hydrolyzable tannins in strawberry plants 控制草莓植物可水解单宁合成的 "加洛酰化-去加洛酰化循环 "中的关键基因

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-16 DOI: 10.1093/hr/uhae350

Lingjie Zhang, Rui Li, Maohao Wang, Qiaomei Zhao, Yifan Chen, Yipeng Huang, Yajun Liu, Xiaolan Jiang, Nana Wang, Tao Xia, Liping Gao

{"title":"Key genes in a “Galloylation-Degalloylation cycle” controlling the synthesis of hydrolyzable tannins in strawberry plants","authors":"Lingjie Zhang, Rui Li, Maohao Wang, Qiaomei Zhao, Yifan Chen, Yipeng Huang, Yajun Liu, Xiaolan Jiang, Nana Wang, Tao Xia, Liping Gao","doi":"10.1093/hr/uhae350","DOIUrl":"https://doi.org/10.1093/hr/uhae350","url":null,"abstract":"Strawberry fruits, known for their excellent taste and potential health benefits, are particularly valued for their rich content of hydrolyzable tannins (HTs). These compounds play key roles in regulating growth and development. However, the molecular mechanisms underlying HT synthesis in plants remains poorly elucidated. In this study, based on a correlation analysis between the transcriptome and metabolome of HTs, galloyl glucosyltransferase (UGT84A22), serine carboxypeptidase-like acyltransferases (SCPL-ATs), and carboxylesterases (CXEs) were screened. Furthermore, in vitro enzymatic assays confirmed that FaSCPL3–1 acted as a hydrolyzable tannins synthase (HTS), catalyzing the continuous galloylation of glucose to form simple gallotannins (GTs). Additionally, FaCXE1/FaCXE3/FaCXE7 catalyzed the degalloylation of simple GTs and ellagitannins (ETs), and FaUGT84A22 catalyzed the glycosylation of gallic acid (GA) to produce 1-O-β-glucogallin (βG), a galloyl donor. Moreover, in FvSCPL3–1-RNAi transgenic strawberry plants, the contents of simple GT and some ET compounds were reduced, whereas, in FaCXE7 overexpressing strawberry plants, these compounds were increased. These enzymes constituted a biosynthetic pathway of galloyl derivatives, termed the “galloylation-degalloylation cycle” (G-DG cycle). Notably, the overexpression of FaCXE7 in strawberry plants not only promoted HT synthesis but also interfered with plant growth and development by reducing lignin biosynthesis. These findings offer new insights into the mechanisms of HT accumulation in plants, contributing to improving the quality of berry fruits quality and enhancing plant resistance.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"37 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MaEIL4-MaMADS36-MaACS7 module transcriptionally regulates ethylene biosynthesis during banana fruit ripening MaEIL4-MaMADS36-MaACS7 模块转录调控香蕉果实成熟过程中的乙烯生物合成

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-16 DOI: 10.1093/hr/uhae345

Maoni Fu, Yunke Zheng, Jing Zhang, Chengju Deng, Jianbin Zhang, Caihong Jia, HongXia Miao, Jingyi Wang, Sijun Zheng, Zhiqiang Jin, Xinguo Li, Jianghui Xie, Juhua Liu

{"title":"The MaEIL4-MaMADS36-MaACS7 module transcriptionally regulates ethylene biosynthesis during banana fruit ripening","authors":"Maoni Fu, Yunke Zheng, Jing Zhang, Chengju Deng, Jianbin Zhang, Caihong Jia, HongXia Miao, Jingyi Wang, Sijun Zheng, Zhiqiang Jin, Xinguo Li, Jianghui Xie, Juhua Liu","doi":"10.1093/hr/uhae345","DOIUrl":"https://doi.org/10.1093/hr/uhae345","url":null,"abstract":"The present research examined the regulatory function of MaEIL4 in the ripening process of banana. The findings demonstrated that MaEIL4 is a transcription factor with activity in the nucleus. The transient modulation of MaEIL4 expression in banana fruit slices has been found to exert a significant impact on maturation, either enhancing or inhibiting its progression, as shown by phenotype and endogenous gene expression. MaEIL4, MaMADS36, and MaACS7 were co-expressed in bananas. MaEIL4 interacted with both the MaMADS36 protein and the TGAA box of the MaMADS36 promoter to activate its expression. Moreover, MaMADS36 bound to the C(A/T)rG box of the MaACS7 promoter to regulate fruit ripening. The results have characterized the mechanism of MaMADS36’s response to upstream ethylene signals and established a new module, MaEIL4-MaMADS36-MaACS7, that transcriptionally regulates banana fruit ripening. This research has enhanced our comprehension of the pivotal function of MaMADS36 in controlling fruit maturation and thus suggests new strategies for fruit shelf-life improvement and post-harvest loss reduction.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"253 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Telomere-to-telomere, gap-free genome of mung bean (Vigna radiata) provides insights into domestication under structural variation

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-14 DOI: 10.1093/hr/uhae337

Kai-Hua Jia, Guan Li, Longxin Wang, Min Liu, Zhi-Wei Wang, Ru-Zhi Li, Lei-Lei Li, Kun Xie, Yong-Yi Yang, Ru-Mei Tian, Xue Chen, Yu-Jun Si, Xiao-Yan Zhang, Feng-Jing Song, Lianzheng Li, Na-Na Li

{"title":"Telomere-to-telomere, gap-free genome of mung bean (Vigna radiata) provides insights into domestication under structural variation","authors":"Kai-Hua Jia, Guan Li, Longxin Wang, Min Liu, Zhi-Wei Wang, Ru-Zhi Li, Lei-Lei Li, Kun Xie, Yong-Yi Yang, Ru-Mei Tian, Xue Chen, Yu-Jun Si, Xiao-Yan Zhang, Feng-Jing Song, Lianzheng Li, Na-Na Li","doi":"10.1093/hr/uhae337","DOIUrl":"https://doi.org/10.1093/hr/uhae337","url":null,"abstract":"Mung bean (Vigna radiata), an essential annual legume, holds substantial value in global agriculture due to its short growth cycle, low input requirements, and nutritional benefits. Despite extensive domestication, the genetic mechanisms underlying its morphological and physiological evolution remain incompletely understood. In this study, we present a gap-free, telomere-to-telomere genome assembly of the mung bean cultivar 'Weilv-9', achieved through the integration of PacBio HiFi, Oxford Nanopore, and Hi-C sequencing technologies. The 500 Mb assembly, encompassing 11 chromosomes and containing 28,740 protein-coding genes, reveals that 49.17% of the genome comprises repetitive sequences. Within the genome, we found the recent amplification of transposable elements significantly impacts the expression of nearby genes. Furthermore, integrating structural variation and SNP data from resequencing, we identified that the fatty acid synthesis, suberin biosynthetic, and phenylpropanoid metabolic processes have undergone strong selection during domestication. These findings provide valuable insights into the genetic mechanisms driving domestication and offer a foundation for future genetic enhancement and breeding programs in mung beans and related species.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"45 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements and strategies of genetic improvement in cassava (Manihot esculenta Crantz): from conventional to genomic approaches 木薯（Manihot esculenta Crantz）遗传改良的进展和战略：从传统方法到基因组方法

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-14 DOI: 10.1093/hr/uhae341

Liang Xiao, Dong Cheng, Wenjun Ou, Xin Chen, Ismail Yusuf Rabbi, Wenquan Wang, Kaimian Li, Huabing Yan

{"title":"Advancements and strategies of genetic improvement in cassava (Manihot esculenta Crantz): from conventional to genomic approaches","authors":"Liang Xiao, Dong Cheng, Wenjun Ou, Xin Chen, Ismail Yusuf Rabbi, Wenquan Wang, Kaimian Li, Huabing Yan","doi":"10.1093/hr/uhae341","DOIUrl":"https://doi.org/10.1093/hr/uhae341","url":null,"abstract":"Cassava (Manihot esculenta Crantz) is a staple food of 800 million people in the tropical and subtropical regions of the world. Its industrial utilization for bioethanol, animal feed, and starch are still continuously expanding. It was not until the 1970s that significant scientific efforts were undertaken to improve cassava, despite its considerable economic and social significance. Shorten the breeding cycle and improve the breeding efficiency are always the focus of the cassava breeding study. In this review, we provide a global perspective on the current status of cassava germplasm resources and explore the diverse applications of cassava breeding methods from hybridization, polyploidy and inbreeding to genomic selection and gene editing. Additionally, we overview at least six nearly complete cassava genome sequences established based on modern genomic techniques. These achievements have substantially supported the advancing of gene discovery and breeding of new cassava varieties. Furthermore, we provide a summary of the advancements in cassava’s functional genomics, concentrating on important traits such as starch quality and content, dry matter content, tolerance to post-harvest physiological deterioration, nutritional quality, and stress resistance. We also provide a comprehensive summary of the milestone events and key advancements in cassava genetic improvement over the past 50 years. Finally, we put forward the perspective of developing genomic selection breeding model and super-hybrids of cassava through building inbreeding population and emphasize the generation of triploid cassavas, allowing cassava to be a tropical model plant serve for basic biological research and molecular breeding.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"83 6 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative Analysis of Genome-wide Association Studies of Polyphenols in Apple Fruits Identifies the MdDof2.4-MdPAT10 Module That Promotes Procyanidin Accumulation

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-12 DOI: 10.1093/hr/uhae349

Zhongxing Li, Cai Gao, Tianle Fan, Yilin Cui, Zeyuan Liu, Lei Li, Qian Qian, Mengjie Cheng, Xiangqiang Zhan, Chundong Niu, Fengwang Ma, Peizhi Yang, Qingmei Guan

{"title":"Integrative Analysis of Genome-wide Association Studies of Polyphenols in Apple Fruits Identifies the MdDof2.4-MdPAT10 Module That Promotes Procyanidin Accumulation","authors":"Zhongxing Li, Cai Gao, Tianle Fan, Yilin Cui, Zeyuan Liu, Lei Li, Qian Qian, Mengjie Cheng, Xiangqiang Zhan, Chundong Niu, Fengwang Ma, Peizhi Yang, Qingmei Guan","doi":"10.1093/hr/uhae349","DOIUrl":"https://doi.org/10.1093/hr/uhae349","url":null,"abstract":"Polyphenols represent a significant class of nutrients in apples, contributing to human health and well-being. Among these, procyanidins stand out as the most prevalent polyphenolic compounds in apple fruits. These compounds are abundant in wild apples and generally low in modern apple cultivars. Therefore, it is crucial to identify and recover genetically lost genes that regulate polyphenol accumulation in order to improve the apple quality. To achieve this, we conducted a genome-wide association study (GWAS) on 15 traits related to polyphenol content, utilizing 134 Malus accessions. We identified 1,204 marker-trait associations (MTAs) and 840 candidate genes, including known polyphenol biosynthetic and regulatory genes, such as MYB7, TT4, and HCT/HQT. Notably, we pinpointed a protein S-acyl transferase 10 (PAT10) which is significantly associated with procyanidin content. Through experiments with transgenic calli, we determined that apple (Malus domestica) MdPAT10 positively regulated procyanidin accumulation. Furthermore, we identified a 51-bp insertion variant (In-868) on the promoter of the PAT10, which influences its expression. Both a yeast one-hybrid (Y1H) assay and an electrophoretic mobility shift assay (EMSA) revealed that MdDof2.4 was able to bind to the promoter of MdPAT10 containing In-868 (MdPAT10proIn-868), but not to the promoter of MdPAT10 without In-868 (MdPAT10pro). Moreover, MdDof2.4 promoted MdPAT10 (with MdPAT10proIn-868) expression and increased procyanidin accumulation in fruits. Overall, our results enhance the understanding of the biosynthetic regulation of apple polyphenols and provide a theoretical foundation and genetic resources for breeding apple varieties with optimal polyphenol content.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"44 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic and transcriptomic analyses provide insight into the variation of floral scent and molecular regulation in different cultivars and flower development of Curcuma alismatifolia

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-12 DOI: 10.1093/hr/uhae348

Chao Song, Jingpu Tian, Dejin Xie, Shengnan Lin, Yingxue Yang, Xiaoni Zhang, Xuezhu Liao, Zhiqiang Wu

{"title":"Metabolomic and transcriptomic analyses provide insight into the variation of floral scent and molecular regulation in different cultivars and flower development of Curcuma alismatifolia","authors":"Chao Song, Jingpu Tian, Dejin Xie, Shengnan Lin, Yingxue Yang, Xiaoni Zhang, Xuezhu Liao, Zhiqiang Wu","doi":"10.1093/hr/uhae348","DOIUrl":"https://doi.org/10.1093/hr/uhae348","url":null,"abstract":"Curcuma alismatifolia is an important ornamental plant of significant economic value, while the floral fragrance has been rarely investigated, leading to a lack of knowledge about the floral scent. By performing metabolomic and transcriptomic analyses, we investigated the variation of 906 volatile organic compounds (VOCs) in florets of eight C. alismatifolia cultivars and four different developmental stages of ‘Chiang Mai Pink’ (CMP). The metabolite profiling revealed that the terpenoid group (213 out of 906) was the predominant VOC, accounting for 33.5% and 43.4% of total VOC contents in the florets of different cultivars and developmental stages, respectively. Sweet and woody were the predominant odors not only in different cultivars but also during developmental stages. The varied intensities of other odors contributed to forming odor diversities in C. alismatifolia floret. We uncovered seven terpenoid synthetase (TPS) genes and four MYB genes of significant association with the biosynthesis of terpenoids in eight cultivars and floret development, respectively. We performed an activity assay on four selected TPS genes and identified that Chr15HA1352 and Chr15HA2528 are responsible for the biosynthesis of α-farnesene. The significant association between the MYB gene (Chr03HA28) and seven terpenoids can be observed among different cultivars and during different developmental stages. These findings highlight the varying floral scents in different cultivars and floret development and suggest the potential roles of identified TPS and MYB genes in the biosynthesis of terpenoids in C. alismatifolia.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"99 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-channel CRISPR-based method for rapid, sensitive detection of four diseases of Brassica rapa in the field

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-12 DOI: 10.1093/hr/uhae351

Xiaojing Liu, Tongbing Su, Xiaoyun Xin, Peirong Li, Weihong Wang, Cancan Song, Xiuyun Zhao, Deshuang Zhang, Yangjun Yu, Jiao Wang, Ning Li, Miao Wang, Fenglan Zhang, Shuancang Yu

{"title":"A multi-channel CRISPR-based method for rapid, sensitive detection of four diseases of Brassica rapa in the field","authors":"Xiaojing Liu, Tongbing Su, Xiaoyun Xin, Peirong Li, Weihong Wang, Cancan Song, Xiuyun Zhao, Deshuang Zhang, Yangjun Yu, Jiao Wang, Ning Li, Miao Wang, Fenglan Zhang, Shuancang Yu","doi":"10.1093/hr/uhae351","DOIUrl":"https://doi.org/10.1093/hr/uhae351","url":null,"abstract":"Pathogens significantly restrict the production of Brassica rapa (Brassica rapa L. ssp. Pekinensis), with climate change and evolving planting patterns exacerbating disease prevalence. Multi-channel rapid diagnostic methods in the field can facilitate the early detection and control of diseases in B. rapa. Here, we established a multi-channel lateral flow biosensor (LFB) combined with a CRISPR/Cas12a cleavage assay for the simultaneous detection of four B. rapa diseases. Key innovations of this study include: (1) High specificity and sensitivity, down to pathogen concentrations of 1.5 pg/μL—due to the optimization of crRNA secondary structure: the more stable the crRNA, the higher its detection sensitivity. (2) Optimized visual detection parameters. We identified ideal concentration ratios for the visual fluorescence detection system: 50 nM Cas12a, 50 nM crRNA, and 500 nM ssDNA fluorescent probe. Furthermore, the optimal concentrations of components on the LFB detection system were 3 μL SA-GNPs, 500 nM ssDNA test strip probe, 0.5 mg/mL biotin-BSA as the test line, and 1 mg/mL anti-FITC as the control line. (3) Field-Ready Cas-AIRPA Platform. We developed the on-site Cas-AIRPA platform for the simultaneous detection of B. rapa pathogens by combining rapid nucleic acid extraction and a four-channel lateral flow biosensor (4-LFB), which quickly provides disease-related information through a specific two-dimensional barcode. Analysis of B. rapa samples in the field confirmed the suitability of the Cas-AIRPA platform for rapid (~25 min) and simultaneous on-site detection of four diseases of B. rapa. This platform can also be adapted to detect other plant diseases in the field.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"11 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0