Horticulture Research最新文献

Harnessing apomixis: natural mechanisms and synthetic innovations for advancing crop and forage breeding 利用无融合：促进作物和饲料育种的自然机制和合成创新

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-16 DOI: 10.1093/hr/uhaf186

Shuyi Hu, Xiaoyun Han, Lei Tian, Kejian Wang, Shuangyan Chen

引用次数: 0

Volatile organic compounds in Solanum lycopersicum leaves and their roles in plant protection 茄叶挥发性有机物及其在植物保护中的作用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-16 DOI: 10.1093/hr/uhaf181

Gargiulo Simona, Ruocco Michelina, Loreto Francesco, Faino Luigi, Monti Maurilia Maria

引用次数: 0

The CsMYB36-CsSWEET17 module mediates the calcium-induced sucrose accumulation in citrus CsMYB36-CsSWEET17模块介导钙诱导的柑橘蔗糖积累

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-16 DOI: 10.1093/hr/uhaf175

Xiawei Sheng, Mengdi Li, Yanrou Luo, Zuolin Mao, Xiawan Zhai, Ji-Hong Liu, Chunlong Li

{"title":"The CsMYB36-CsSWEET17 module mediates the calcium-induced sucrose accumulation in citrus","authors":"Xiawei Sheng, Mengdi Li, Yanrou Luo, Zuolin Mao, Xiawan Zhai, Ji-Hong Liu, Chunlong Li","doi":"10.1093/hr/uhaf175","DOIUrl":"https://doi.org/10.1093/hr/uhaf175","url":null,"abstract":"Sugar content serves as a crucial determinant of fruit flavor quality and nutritional value. Calcium plays extensive regulatory roles in fruit development and quality formation, yet the molecular mechanisms underlying calcium-mediated sugar accumulation remain poorly understood. In this study, we demonstrate that calcium treatment enhances sugar accumulation in both citrus fruits and calli, concomitant with upregulated expression of the sucrose transporter gene CsSWEET17. Functional characterization revealed that the membrane-localized CsSWEET17 protein exhibits sucrose transport activity. Transgenic overexpression of CsSWEET17 in citrus juice sacs, calli, and heterologous tomato systems consistently elevated sucrose levels. Conversely, suppression of CsSWEET17 expression through either virus-induced gene silencing (VIGS) or RNA interference (RNAi) significantly reduced sucrose content in citrus. Further investigation identified CsMYB36 as a calcium-responsive transcription factor that directly activates CsSWEET17 expression. Transgenic validation demonstrated that both calcium signaling and CsMYB36-mediated sucrose accumulation strictly depend on CsSWEET17 transcriptional regulation. Our findings elucidate a novel calcium-MYB36-SWEET17 regulatory module controlling sucrose accumulation, providing molecular insights for calcium-based strategies in citrus quality improvement and informing fundamental mechanisms of sugar transporter regulation in fruit crops.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"9 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Slow and steady wins the race: the negative regulators of ethylene biosynthesis in horticultural plants 缓慢而稳定的赢得比赛：园艺植物中乙烯生物合成的负调节因子

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-16 DOI: 10.1093/hr/uhaf108

Dongdong Li, Shuang Zeng, Ruyi Dai, Kunsong Chen

{"title":"Slow and steady wins the race: the negative regulators of ethylene biosynthesis in horticultural plants","authors":"Dongdong Li, Shuang Zeng, Ruyi Dai, Kunsong Chen","doi":"10.1093/hr/uhaf108","DOIUrl":"https://doi.org/10.1093/hr/uhaf108","url":null,"abstract":"The gaseous hormone ethylene controls a variety of physiological processes in horticultural plants, including fruit ripening and elongation, flower development and senescence, and responses to stresses. The functions of ethylene in these processes are intimately linked to its precise biosynthesis, which is finely tuned by a complex network of positive and negative regulators. While significant progress has been made in understanding the roles of positive regulators in ethylene biosynthesis, the negative regulators of ethylene biosynthesis has only recently begun to receive more focus. Ethylene biosynthesis is a simple two-step reaction in land plants, committed by two dedicated enzymes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO). Over the past decade, a growing number of research has identified a wide range of transcriptional, posttranscriptional and epigenetic negative regulators for ACS and/or ACO in horticultural plants, greatly enhancing our understanding of the intricate network that modulates ethylene production. In this review, we provide a comprehensive overview of the negative regulators that mediate ethylene biosynthesis in horticultural plants, with respect to their functions and molecular mechanisms, and their responses to external environmental stimuli or internal growth signals.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"9 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H2S promotes flowering in Brassica rapa ssp. pekinensis by persulfidation of the splicing factor BraATO2 H2S促进油菜开花。剪接因子BraATO2的过硫化作用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-16 DOI: 10.1093/hr/uhaf190

Xuefeng Hao, Weier Li, Haiyan Cao, Fulin Tang, Tian Ma, Jiao Zhang, Liping Zhang, Limei Chen, Zhuping Jin, Yanxi Pei

{"title":"H2S promotes flowering in Brassica rapa ssp. pekinensis by persulfidation of the splicing factor BraATO2","authors":"Xuefeng Hao, Weier Li, Haiyan Cao, Fulin Tang, Tian Ma, Jiao Zhang, Liping Zhang, Limei Chen, Zhuping Jin, Yanxi Pei","doi":"10.1093/hr/uhaf190","DOIUrl":"https://doi.org/10.1093/hr/uhaf190","url":null,"abstract":"Hydrogen sulfide (H2S) is a newly identified gasotransmitter that plays an irreplaceable physiological role in plant growth, development, and environmental responses through persulfidation of cysteine (Cys) residues (RSSH). However, reports on the direct RSSH targets of H2S in plants remain limited. The flowering regulation mechanisms of Brassica rapa ssp. pekinensis are a significant scientific issue in the crop production industry, however they remain poorly understood. BraATO2 is an important splicing factor in genetic alternative splicing (AS). Our study demonstrated that H2S regulated BraATO2 function by persulfidating the Cys residue at position 416. In turn, this influenced the AS patterns of multiple genes in B. rapa, specifically the flowering regulator BraAGL31/MAF2 within the FLOWERING LOCUS C-like (FLC-like) gene family, causing accelerated flowering. This study identified a new direct target of H2S and uncovered a novel pathway influencing flowering in B. rapa. Furthermore, the study findings provide fresh insights into the development of innovative flowering regulators for plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"23 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First interspecific multi-parent advanced generation inter-cross (MAGIC) population in Capsicum peppers: development, phenotypic evaluation, genomic analysis, and prospects 辣椒的首个种间多亲本杂交（MAGIC）群体：发育、表型评价、基因组分析与展望

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-15 DOI: 10.1093/hr/uhaf182

Neus Ortega-Albero, Miguel Díaz-Riquelme, Luciana Gaccione, Lorenzo Barchi, Ana Fita, Adrián Rodríguez-Burruezo

{"title":"First interspecific multi-parent advanced generation inter-cross (MAGIC) population in Capsicum peppers: development, phenotypic evaluation, genomic analysis, and prospects","authors":"Neus Ortega-Albero, Miguel Díaz-Riquelme, Luciana Gaccione, Lorenzo Barchi, Ana Fita, Adrián Rodríguez-Burruezo","doi":"10.1093/hr/uhaf182","DOIUrl":"https://doi.org/10.1093/hr/uhaf182","url":null,"abstract":"This work presents the first eight-way multi-parental advanced generation inter-cross (MAGIC) population in pepper. This interspecific MAGIC population was built with six Capsicum annuum accessions and two C. chinense accessions, selected for encompassing a representative and wide genetic diversity, and being complementary for morphological, agronomic, and fruit quality traits. The population in its third selfing generation has been phenotyped with reliable descriptors and genotyped using genotyping-by-sequencing (GBS) to assess its overall diversity, homozygosity, parental contributions, and genetic structure. A great variability was found in the phenotyping study, showing many forms of recombination of all the founder lines. Even more, new phenotypic combinations were found, as well as transgressive inheritance in quantitative traits. The S3-generation contained a balanced distribution of the parental genomes and each S3-individual seemed to contain a unique genomic combination of the founder lines reaching a high homozygosity. In this regard, a preliminary genome-wide association study (GWAS) was performed for highly heritable traits to evaluate the potential of this population for future breeding prospects. Strong associations were found for most traits analysed, like stem pubescence and fruit colour at maturity stage, with associated genes related to response to stress and defence functions; or fruit wall consistency, with associated genes related to lipid metabolism. Our results show that this first Capsicum MAGIC population is a valuable genetic resource for research and breeding purposes in peppers, by identifying genomic regions associated to traits of interest and its potential for future GWAS in more complex agronomical and fruit quality traits.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"48 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the genetic basis for enhanced mushroom flavor in Quercus fabri through genome sequencing and metabolic profiling 通过基因组测序和代谢谱分析揭示了栎香菇风味增强的遗传基础

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-09 DOI: 10.1093/hr/uhaf156

Liwen Wu, Yuqing Cai, Chenggang Jiang, Xiang Shi, Shifa Xiong, Yicun Chen, Yangdong Wang

{"title":"Uncovering the genetic basis for enhanced mushroom flavor in Quercus fabri through genome sequencing and metabolic profiling","authors":"Liwen Wu, Yuqing Cai, Chenggang Jiang, Xiang Shi, Shifa Xiong, Yicun Chen, Yangdong Wang","doi":"10.1093/hr/uhaf156","DOIUrl":"https://doi.org/10.1093/hr/uhaf156","url":null,"abstract":"Quercus fabri is a common timber oak tree species widely distributed in subtropical areas of China. In this study, we presented a chromosome-scale reference genome assembly of Q. fabri achieved by integrating PacBio Sequel II, DNBseqTM and Hi-C sequencing platforms, and the results indicated the Q. fabri genome has a size of 836.74 Mb. Through the analysis of significantly expanded gene families, we identified that many of the top-ranked KEGG pathways are associated with amino acid metabolism. Subsequently, we performed an amino acid metabolic profile analysis on Q. fabri and related species, including Q. aliena, Q. acutissima, and Q. variabilis. The findings revealed that the content of amino acids in Q. fabri was significantly higher than that in the other three oak species. Additionally, we found a significantly higher content of flavor amino acids, such as glutamic acid (Glu), aspartic acid (Asp), and glycine (Gly), in Q. fabri. Considering these results, we designed experiments to assess the nutrient content in mushrooms cultivated from the four oak trees. The results indicated that the total amino acid and protein content of mushrooms cultivated using Q. fabri as a substrate was significantly greater than that of mushrooms grown on the other three oak species. This characteristic may explain why Q. fabri wood is particularly effective as a substrate for cultivating more flavorful mushrooms. This study presents the complete genome and evolutionary information of Q. fabri, and integrates metabolic profiling to explore the underlying reasons for the enhanced flavor of mushrooms cultivated from it.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"4 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144594126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SlALKBH9B is involved in drought-induced flower drop by regulating ethylene production SlALKBH9B通过调节乙烯的产生参与干旱诱导的花落

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-07 DOI: 10.1093/hr/uhaf173

Yue Cai, Lina Cheng, Xianfeng Liu, Ruizhen Li, Yang Liu, Siqi Ge, Sai Wang, Jing Liu, Changhua Tan, Sida Meng, Mingfang Qi, Cai-Zhong Jiang, Tianlai Li, Tao Xu

{"title":"SlALKBH9B is involved in drought-induced flower drop by regulating ethylene production","authors":"Yue Cai, Lina Cheng, Xianfeng Liu, Ruizhen Li, Yang Liu, Siqi Ge, Sai Wang, Jing Liu, Changhua Tan, Sida Meng, Mingfang Qi, Cai-Zhong Jiang, Tianlai Li, Tao Xu","doi":"10.1093/hr/uhaf173","DOIUrl":"https://doi.org/10.1093/hr/uhaf173","url":null,"abstract":"Drought induces tomato (Solanum lycopersicum) flowers and fruits drop, which causes serious yield and economic losses in agriculture. However, the mechanism of action remains unclear. N6-methyladenosine (m6A) methylation is a prevalent epigenetic change integral to the growth, development, and adaptation of plants to abiotic stress factors. However, whether it participates in drought-induced abscission remains to be further studied. Here, we report that tomato demethylase alpha-ketoglutarate-dependent dioxygenase B (AlkB) homolog 9B (SlALKBH9B) exerts a detrimental influence on the regulation of drought-induced flower drop by mediating ethylene production. We found that drought markedly reduced the expression of SlALKBH9B, and knockout of SlALKBH9B enhanced flower drop, while overexpression of SlALKBH9B delayed the flower drop. Under drought conditions, the ethylene production of Slalkbh9b exhibited a considerably greater yield than that of the wild type (WT), while SlALKBH9B overexpression plants had lower ethylene production. Application of ethylene could abolish the delayed abscission effect of overexpression of SlALKBH9B. Further studies showed that drought downregulated SlALKBH9B expression, which specifically enhanced the methylation level of the 3′ untranslated region (UTR) of tomato ethylene excess producer 1 (SlETO1), leading to a decrease in the stability of SlETO1 mRNA and its protein translation efficiency. The loss of SlETO1 resulted in the accumulation of tomato 1-aminocyclopropane-1-carboxylic acid synthase 3 (SlACS3) and SlACS8 in the abscission zone (AZ) and then boosted ethylene production to accelerate abscission. Our results show that SlALKBH9B is an important inhibitor for drought-induced abscission and reveal a new mechanism through which drought-enhanced ethylene production leads to flower drop.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"92 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CsCOI1 regulates plant growth and defense in citrus CsCOI1调控柑橘植物的生长和防御

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-07 DOI: 10.1093/hr/uhaf174

Gang Hu, Bing Liu, Kun Yang, Wei-Kang Zheng, Yi Zhang, Cai-Xia Teng, Duo-Yi Huang, Ruo-Hao Yan, Michitaka Notaguchi, Munenori Kitagawa, Zong-Cheng Lin, Qiang Xu

{"title":"CsCOI1 regulates plant growth and defense in citrus","authors":"Gang Hu, Bing Liu, Kun Yang, Wei-Kang Zheng, Yi Zhang, Cai-Xia Teng, Duo-Yi Huang, Ruo-Hao Yan, Michitaka Notaguchi, Munenori Kitagawa, Zong-Cheng Lin, Qiang Xu","doi":"10.1093/hr/uhaf174","DOIUrl":"https://doi.org/10.1093/hr/uhaf174","url":null,"abstract":"Jasmonates (JAs) play essential roles in plant development and defense. JA perception and responses remain elusive in citrus. Here, we identified core components for JA perception in citrus and elucidated transcriptional changes associated with JA signaling in growth and defense. We showed the F-box protein CORONATINE INSENSITIVE1 (COI1) in citrus is a JA receptor, as CsCOI1 mutants are insensitive to methyl jasmonate and CsCOI1 interacts with CsJAZs in the presence of JA-Ile. CsCOI1-mediated JA signaling represses shoot growth while enhancing resistance to insects. Consistently, CsCOI1 represses the expression of growth promoting genes such as PIF7, while upregulating genes related to defense metabolites in terpene and flavonoid pathways. Additionally, JA signaling antagonizes salicylic acid (SA) signaling at the transcriptional level and promotes susceptibility to citrus canker disease. This study highlights the role of JA signaling in balancing growth and resistance to biotic stress in citrus, revealing critical trade-offs for consideration in precision citrus breeding.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"20 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MaASR3-MaHDT1 module modulates high temperature-inhibited chlorophyll breakdown in banana fruit by suppressing the E3 ligase MaNIP1 MaASR3-MaHDT1模块通过抑制E3连接酶MaNIP1调节香蕉果实中高温抑制的叶绿素分解

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-07-07 DOI: 10.1093/hr/uhaf172

Qi Luo, Wei Wei, Yu-mei Zhang, Jian-fei Kuang, Jian-ye Chen, Wang-jin Lu, Zhi-jun Cai, Wei Shan

{"title":"The MaASR3-MaHDT1 module modulates high temperature-inhibited chlorophyll breakdown in banana fruit by suppressing the E3 ligase MaNIP1","authors":"Qi Luo, Wei Wei, Yu-mei Zhang, Jian-fei Kuang, Jian-ye Chen, Wang-jin Lu, Zhi-jun Cai, Wei Shan","doi":"10.1093/hr/uhaf172","DOIUrl":"https://doi.org/10.1093/hr/uhaf172","url":null,"abstract":"The ripening of banana fruit at high temperature (HT) exceeding 24 °C impedes developing yellow peels, causing green ripening, which considerably lowers its marketability. Our recent study found that HT induces E3 ubiquitin ligase MaNIP1 (NYC1 interacting protein 1)-mediated degradation of MaNYC1 (NON-YELLOW COLORING 1) to inhibit chlorophyll breakdown during banana fruit ripening, but MaNIP1's upstream regulatory mechanism is still unclear. Herein, the ASR transcription factor (TF) MaASR3, which is repressed in green-ripened fruit compared to yellow-ripened fruit, was identified as the potential binding protein for the MaNIP1 promoter. MaASR3 promoted chlorophyll degradation in banana fruit by repressing MaNIP1 expression. More importantly, the histone deacetylase MaHDT1 interacted with MaASR3 and enhanced MaASR3-mediated repression of MaNIP1. Overexpression of MaASR3 in banana fruit reduced the histone acetylation levels in the MaNIP1 promoter and repressed MaNIP1 expression, thereby weakening the HT-inhibited de-greening of banana fruit. Our study reveals an innovative regulatory cascade comprising the MaASR3-MaHDT1-MaNIP1 complex, which modulates HT-inhibited chlorophyll degradation. This explains the green ripening in bananas exposed to such conditions and enhances the comprehension of transcriptional and epigenetic regulations of fruit quality deterioration due to temperature stresses.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"20 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144568568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0