Horticulture Research最新文献

The MabZIP5–MaMYB69 module cooperates with MaERF55 to modulate banana fruit ripening via cell wall degradation MabZIP5-MaMYB69模块与MaERF55合作，通过细胞壁降解调节香蕉果实成熟

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-17 DOI: 10.1093/hr/uhaf275

Fan Liu, Xueli Sun, Jianzhu Cao, Ou Sheng, Tongxin Dou, Qiaosong Yang, Chunhua Hu, Guiming Deng, Weidi He, Huijun Gao, Tao Dong, Chunyu Li, Yaoyao Li, Cancan Liu, Ganjun Yi, Fangcheng Bi

{"title":"The MabZIP5–MaMYB69 module cooperates with MaERF55 to modulate banana fruit ripening via cell wall degradation","authors":"Fan Liu, Xueli Sun, Jianzhu Cao, Ou Sheng, Tongxin Dou, Qiaosong Yang, Chunhua Hu, Guiming Deng, Weidi He, Huijun Gao, Tao Dong, Chunyu Li, Yaoyao Li, Cancan Liu, Ganjun Yi, Fangcheng Bi","doi":"10.1093/hr/uhaf275","DOIUrl":"https://doi.org/10.1093/hr/uhaf275","url":null,"abstract":"The MYB protein family comprises numerous transcription factors with important functions in various biological processes in plants; however, their role in modulating banana fruit ripening has been rarely investigated. In this study, we identified an R2R3-type MYB gene, MaMYB69, which promotes fruit ripening by directly modulating cell wall degradation. The expression of MaMYB69, which encodes a nucleus-localized transcription activator, was induced by ethylene and upregulated during banana ripening. MaMYB69 activated the expression of MaPE, MaPL1, MaGAL, and MaPG3 by directly targeting their promoters. MaMYB69 overexpression in tomato and banana accelerated fruit ripening and stimulated cell wall-modifying gene expression. Moreover, MaMYB69 interacted with MaERF55 and strengthened its transactivation activity through downstream target genes. MabZIP5, an aroma biosynthesis regulator, acted directly upstream of MaMYB69, enhancing its transcription. MaMYB69 also formed a homodimer with itself and activated its expression. Collectively, our results show that MaMYB69 is pivotal in controlling banana ripening and softening and improve our understanding of the regulatory cascades involved in fruit ripening. MaMYB69 may serve as a potential target for improving fruit quality and extending the shelf life.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"14 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Odor to Order: Unveiling the Crucial Role of Hydrogen Sulfide in Plant Life 从气味到秩序：揭示硫化氢在植物生命中的关键作用

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-17 DOI: 10.1093/hr/uhaf273

Zhuping Jin

引用次数: 0

The vacuolar transporters MaMATE11 and MaMATE14 affect blue flower coloration in grape hyacinth (Muscari) 空泡转运蛋白MaMATE11和MaMATE14对葡萄风信子蓝花颜色的影响

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-17 DOI: 10.1093/hr/uhaf270

Xiaoyun Cao, Jingwen Xie, Xuelan Gao, Wanqi Pan, Jiaxin Gong, Lingjuan Du

{"title":"The vacuolar transporters MaMATE11 and MaMATE14 affect blue flower coloration in grape hyacinth (Muscari)","authors":"Xiaoyun Cao, Jingwen Xie, Xuelan Gao, Wanqi Pan, Jiaxin Gong, Lingjuan Du","doi":"10.1093/hr/uhaf270","DOIUrl":"https://doi.org/10.1093/hr/uhaf270","url":null,"abstract":"The development of blue flower coloration involves the biosynthesis, transport, and accumulation of flavonoids in petal epidermal cells. Although the mechanisms of flavonoid biosynthesis and regulation are well understood, much less is known about the molecular basis of vacuolar anthocyanin/flavonoid sequestration. Here, we identified two tonoplast-localized MATE transporters, MaMATE11 and MaMATE14, that participate in flavonoid transport and influence the blue color of grape hyacinth petals. In vitro transport experiments revealed that both proteins transported a range of flavonoid substrates, with a preference for malonylated anthocyanins, but differed in their substrate specificity and kinetic parameters. Both MaMATE11 and MaMATE14 could complement the anthocyanin-deficient phenotype of the Arabidopsis AtDTX35 mutant, and silencing of either gene by RNA interference significantly reduced anthocyanin accumulation in petals of grape hyacinth. Expression of MaMATE11 and MaMATE14 was directly activated by the anthocyanin-biosynthesis-related transcription factors MaMybA and MaAN2, respectively, establishing a coordinated anthocyanin synthesis–transport module. These findings provide insight into mechanisms of floral coloration and flavonoid translocation in blue-pigmented species and identify valuable target genes for molecular breeding of ornamental flower colors.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"48 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whitening Fruit by CRISPR/Cas9-Mediated Homoeolog-Specific Gene Editing of MYB10-1B in Strawberry (F. ×ananassa) CRISPR/ cas9介导的草莓MYB10-1B同源特异性基因编辑美白果实（F. ×ananassa）

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-15 DOI: 10.1093/hr/uhaf272

Man Bo Lee, Yoon Jeong Jang, Hyeondae Han, Kanika Saxena, Youngjae Oh, Jae Yoon Kim, Seonghee Lee

{"title":"Whitening Fruit by CRISPR/Cas9-Mediated Homoeolog-Specific Gene Editing of MYB10-1B in Strawberry (F. ×ananassa)","authors":"Man Bo Lee, Yoon Jeong Jang, Hyeondae Han, Kanika Saxena, Youngjae Oh, Jae Yoon Kim, Seonghee Lee","doi":"10.1093/hr/uhaf272","DOIUrl":"https://doi.org/10.1093/hr/uhaf272","url":null,"abstract":"Fruit color is a key quality trait in strawberry breeding and cultivar development, as it directly influences consumer preference and marketability. Anthocyanins are the pigments responsible for the red coloration in strawberries, and the transcription factor MYB10 gene plays a crucial role in regulating the anthocyanin biosynthetic pathway. Our previous study identified a homoeolog-specific copy, MYB10-1B, located on chromosome 1B, as a key regulator of fruit color. The natural mutation in MYB10-1B, such as in the variety ‘Florida Pearl’ leads to the development of white fruit. Building on this discovery, we applied CRISPR/Cas9-mediated homoeolog-specific editing to target the functional dominant allele, MYB10-1B, in the cultivated octoploid strawberry ‘Florida Brilliance’, successfully altering the fruit color from red to white. Gene expression analysis in the edited lines revealed down-regulation of MYB10-1B and key anthocyanin biosynthesis genes (CHS, DFR, and ANS). Furthermore, whole genome re-sequencing results showed precise on-target mutations in MYB10-1B with minimal off-target effects. This study highlights the successful application of homoeolog-specific CRISPR/Cas9-mediated gene editing in polyploid species and provides a foundation for functional genomics and advanced breeding strategies in strawberries. Importantly, our findings demonstrate that specific targeting the dominantly expressed homoeologous copy is essential for inducing phenotypic changes in polyploids. This underscores the importance of precise gene editing in octoploid strawberry to drive trait improvement.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"110 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145295601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aphid Adaptation to Plant Secondary Metabolites: Adaptive Mechanism of Resistance Evolution and Future Prospects 蚜虫对植物次生代谢物的适应：适应性机制、抗性进化及未来展望

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-13 DOI: 10.1093/hr/uhaf267

Muhammad Farhan, Jilong Pan, Jun Zhao, Hanjing Yang, Shuai Zhang

{"title":"Aphid Adaptation to Plant Secondary Metabolites: Adaptive Mechanism of Resistance Evolution and Future Prospects","authors":"Muhammad Farhan, Jilong Pan, Jun Zhao, Hanjing Yang, Shuai Zhang","doi":"10.1093/hr/uhaf267","DOIUrl":"https://doi.org/10.1093/hr/uhaf267","url":null,"abstract":"Aphids are demonstrated to be voracious phloem feeders, among the most damaging insect pests, due to their capacity to decrease crop production and vector plant viruses. Plant secondary metabolites (PSMs) comprise an essential element of plant protection, which in most cases deters and affects aphid performance. Nonetheless, aphids have developed various resistance mechanisms to counteract these chemicals. This review provides an extensive overview of the biological and molecular adaptations that aphids employ to counteract PSMs, including enzymatic detoxification, antioxidant defense, sequestration, behavioral response shifts, suppression of plant defense mechanisms by symbionts, and manipulation of host signaling pathways by effector proteins. We also described the suppression of the defense pathways by aphid-associated viruses, which further complicates plant-aphid interactions. Although significant insights have been gained about each of the individual mechanisms, research gaps remain, particularly in the functional confirmation of detox genes, the communication interactions of the symbionts, and whether sequestration could play an ecological role across species. Intensive efforts involving molecular-based breeding of horticultural crops, as well as traditional breeding with wild relatives highly endowed with aphid-resistant PSM traits, should be employed in the future to provide sustainable crop protection. New technologies in crop genomics, the identification of effectors, and microbiome research promise the development of resistant cultivars that are not only resistant to aphids but also prevent the spread of disease by their vectors. Together, all this knowledge has the potential to produce high-yielding crops that are resistant to aphids and to implement sustainable farming practices.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"86 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aphid Adaptation to Plant Secondary Metabolites: Adaptive Mechanism of Resistance Evolution and Future Prospects 蚜虫对植物次生代谢物的适应：适应性机制、抗性进化及未来展望

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-09 DOI: 10.1093/hr/uhaf269

Muhammad Farhan, Jilong Pan, Jun Zhao, Hanjing Yang, Shuai Zhang

{"title":"Aphid Adaptation to Plant Secondary Metabolites: Adaptive Mechanism of Resistance Evolution and Future Prospects","authors":"Muhammad Farhan, Jilong Pan, Jun Zhao, Hanjing Yang, Shuai Zhang","doi":"10.1093/hr/uhaf269","DOIUrl":"https://doi.org/10.1093/hr/uhaf269","url":null,"abstract":"Aphids are demonstrated to be voracious phloem feeders, among the most damaging insect pests, due to their capacity to decrease crop production and vector plant viruses. Plant secondary metabolites (PSMs) comprise an essential element of plant protection, which in most cases deters and affects aphid performance. Nonetheless, aphids have developed various resistance mechanisms to counteract these chemicals. This review provides an extensive overview of the biological and molecular adaptations that aphids employ to counteract PSMs, including enzymatic detoxification, antioxidant defense, sequestration, behavioral response shifts, suppression of plant defense mechanisms by symbionts, and manipulation of host signaling pathways by effector proteins. We also described the suppression of the defense pathways by aphid-associated viruses, which further complicates plant-aphid interactions. Although significant insights have been gained about each of the individual mechanisms, research gaps remain, particularly in the functional confirmation of detox genes, the communication interactions of the symbionts, and whether sequestration could play an ecological role across species. Intensive efforts involving molecular-based breeding of horticultural crops, as well as traditional breeding with wild relatives highly endowed with aphid-resistant PSM traits, should be employed in the future to provide sustainable crop protection. New technologies in crop genomics, the identification of effectors, and microbiome research promise the development of resistant cultivars that are not only resistant to aphids but also prevent the spread of disease by their vectors. Together, all this knowledge has the potential to produce high-yielding crops that are resistant to aphids and to implement sustainable farming practices.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"38 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inducible MdAGG lectins in apple immunity toward Fire Blight: CRISPR/Cas9 validation and their potential for intragenesis approaches 诱导型magg凝集素在苹果对火疫病免疫中的作用：CRISPR/Cas9验证及其内遗传方法的潜力

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-03 DOI: 10.1093/hr/uhaf262

Antoine Bodelot, Nicolas Dousset, Elisa Ravon, Christelle Heintz, Marie-Noelle Brisset, Alexandre Degrave, Emilie Vergne

{"title":"Inducible MdAGG lectins in apple immunity toward Fire Blight: CRISPR/Cas9 validation and their potential for intragenesis approaches","authors":"Antoine Bodelot, Nicolas Dousset, Elisa Ravon, Christelle Heintz, Marie-Noelle Brisset, Alexandre Degrave, Emilie Vergne","doi":"10.1093/hr/uhaf262","DOIUrl":"https://doi.org/10.1093/hr/uhaf262","url":null,"abstract":"Fire blight, caused by the bacterium Erwinia amylovora, represents a significant threat to apple (Malus domestica) production. Currently, only a limited number of genes effectively involved in resistance to E. amylovora have been identified. Seeking new resistance candidates, we focused on a multigene family encoding amaranthin-like lectins, which are highly upregulated following chemical elicitation by acibenzolar-S-methyl (ASM). These lectins are believed to contribute to downstream defense by promoting bacterial aggregation, which led to their designation as Malus domestica agglutinins (MdAGG). When loss-of-function editions were introduced into MdAGG genes, the plant's ability to mount a fully effective defense response against fire blight upon ASM treatment was compromised, confirming the role of MdAGGs in fire blight resistance. Next, we coupled the pPPO16 promoter, endogenous to apple and known to be rapidly induced during E. amylovora infection, with the coding sequence of MdAGG10 to create apple lines with fire blight-inducible MdAGG10 expression. Early MdAGG10 expression in these lines significantly improved resistance to fire blight, and an additional ASM treatment further enhanced this resistance. In summary, we conclude that MdAGGs act as defense genes whose timely expression can provide effective resistance against E. amylovora.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"37 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and Epigenomic coordination maintains subgenome transcriptional balance in Allotetraploid Brassica napus 基因组和表观基因组协调维持异源四倍体甘蓝型油菜亚基因组转录平衡

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-03 DOI: 10.1093/hr/uhaf266

Jie Zhou, Meng Ma, Qing Zhang, Shangyan Ni, Hu Zhao, Jing Wen, Jinxiong Shen, Tingdong Fu, Lun Zhao

{"title":"Genomic and Epigenomic coordination maintains subgenome transcriptional balance in Allotetraploid Brassica napus","authors":"Jie Zhou, Meng Ma, Qing Zhang, Shangyan Ni, Hu Zhao, Jing Wen, Jinxiong Shen, Tingdong Fu, Lun Zhao","doi":"10.1093/hr/uhaf266","DOIUrl":"https://doi.org/10.1093/hr/uhaf266","url":null,"abstract":"Allopolyploids have successfully overcome ‘genome shock’, yet how their subgenomes adapt to coexistence remains largely unclear. Here, we constructed high-resolution epigenomic maps for the diploids Brassica rapa (ArAr) and B. oleracea (CoCo), and examined epigenomic variation in the allotetraploid B. napus (AnAnCnCn) relative to its putative progenitors. We discovered that coordinated genomic and epigenomic reprogramming in B. napus drove convergence of sequence and epigenomic features between An and Cn, significantly reducing expression divergence in homoeologs. Convergent homoeologs were functionally enriched in pathways related to genome stability and abiotic stress responses. Notably, Cn in B. napus exhibited greater sequence conservation and epigenetic homeostasis. Furthermore, transcription factor binding sites (TFBSs) affected by genomic variation in An showed convergent regulatory changes toward Cn, indicating that allopolyploids mitigate subgenomic conflicts through multi-layered regulatory coordination. In conclusion, coordinated genomic and epigenomic convergence provides critical insights into the stability and adaptive evolution of allopolyploids.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"7 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amino Acid Transporter CsBAT Links GABA Accumulation to Flavonoid Metabolism in Camellia sinensis 氨基酸转运蛋白CsBAT与山茶中GABA积累与类黄酮代谢有关

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-01 DOI: 10.1093/hr/uhaf261

Lin Feng, Panpan Liu, Yuanyuan He, Shengpeng Wang, Rui Luo, Anhui Gui, Jinjin Xue, Shiwei Gao, Pengcheng Zheng

{"title":"Amino Acid Transporter CsBAT Links GABA Accumulation to Flavonoid Metabolism in Camellia sinensis","authors":"Lin Feng, Panpan Liu, Yuanyuan He, Shengpeng Wang, Rui Luo, Anhui Gui, Jinjin Xue, Shiwei Gao, Pengcheng Zheng","doi":"10.1093/hr/uhaf261","DOIUrl":"https://doi.org/10.1093/hr/uhaf261","url":null,"abstract":"γ-Aminobutyric acid (GABA), a signature bioactive compound in tea, plays a crucial role in determining both flavor profile and health-promoting properties. Despite its importance, the molecular regulation of GABA accumulation in tea plants - especially its metabolic crosstalk with key quality determinants like flavonoids - remains elusive. While amino acid transporters are known to mediate source-sink allocation in plants, the functional characterization of GABA transporters in Camellia sinensis has been lacking. In this study, we identified and functionally characterized the bidirectional amino acid transporter CsBAT in tea plants. Through a comprehensive multi-platform validation system encompassing yeast heterologous expression, Arabidopsis genetic transformation, and tea transgenic system, we revealed that CsBAT shows vascular-specific expression patterns and facilitates directional amino acid transport from source (mature leaves) to sink (young shoots), thereby significantly boosting GABA accumulation in buds and young leaves. Importantly, we discovered that CsBAT functionally interacts with key flavonoid biosynthetic enzymes (LAR, 4CL, C4H) within secondary metabolic networks. Our findings provide the first mechanistic link between CsBAT-mediated amino acid transport and tea quality formation, establishing both theoretical frameworks and practical tools for molecular breeding of premium tea cultivars.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"28 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative chromosome-level genomics and metabolomics uncover regulatory networks linking monoterpenoid biosynthesis and glandular trichome formation in Mosla chinensis 染色体水平的整合基因组学和代谢组学揭示了中华藓单萜类生物合成和腺毛形成之间的调控网络

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-10-01 DOI: 10.1093/hr/uhaf263

Muyao Yu, Chenyi Li, Xiaoqing Wang, Dan Jiang, Xueqing Fu, Chao Chen, Guangxi Ren, Xuewei Li, Yaojie Zhang, Qi Liu, Shuyi Qian, Yang Han, Xiaoqun He, Zhenfang Bai, Badalahu Tai, Luqi Huang, Jinbao Yu, Han Zheng, Chunsheng Liu

{"title":"Integrative chromosome-level genomics and metabolomics uncover regulatory networks linking monoterpenoid biosynthesis and glandular trichome formation in Mosla chinensis","authors":"Muyao Yu, Chenyi Li, Xiaoqing Wang, Dan Jiang, Xueqing Fu, Chao Chen, Guangxi Ren, Xuewei Li, Yaojie Zhang, Qi Liu, Shuyi Qian, Yang Han, Xiaoqun He, Zhenfang Bai, Badalahu Tai, Luqi Huang, Jinbao Yu, Han Zheng, Chunsheng Liu","doi":"10.1093/hr/uhaf263","DOIUrl":"https://doi.org/10.1093/hr/uhaf263","url":null,"abstract":"Xiangru, with Mosla chinensis (Mc, 2n=18) and its considered cultivar M. chinensis ‘Jiangxiangru’ (McJ, 2n=18) as original plants, are annual herbs of the Lamiaceae family, and is widely used as medicinal and edible plant due to its spleen strengthening function. However, absence of genomic resource impedes in-depth research towards Xiangru. In this study, the morphological characteristics and volatile organic compounds (VOC) contents of Mc and McJ were analyzed, showing higher trichome density and monoterpenoids accumulation obtained in Mc, whereas McJ possessed higher biomass. We assembled high-quality Mc, McJ and their adulterant M. soochowensis (2n=18) genomes of 426.1, 408.8 and 412.8 Mb, respectively, containing the repeat sequences of 57.17%, 56.33%, and 55.83%. Comparative genomics indicating Mosla radiating ~13.3 Mya, supporting McJ initially as a natural naturally formed resource. Five monoterpene synthase genes were identified through comparative transcriptome, and were responsible for catalyzing production of diversified monoterpene skeleton, in which TPS1 mediate formation of γ-terpinene, accompanied by CYP71D179 and SDR2 leading to the final production of carvacrol and thymol. We further explore correlation between monoterpenoids biosynthesis and trichome development, indicating MIXTA and WIN1 jointly regulate both trichome formation and VOC accumulation by directly binding promotors of TPS1 and CYP71D179, respectively. Our study fills vacancy of genus Mosla genomes, improving the biosynthetic and regulatory mechanism of volatile compounds in aromatic Traditional Chinese Medicine, also offering novel targets for quality-directed breeding in Xiangru.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"114 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0