Horticulture Research最新文献_第2页

Tea polyphenol mediated CsMYB77 regulation of CsPOD44 to promote tea plant (Camellia sinensis) root drought resistance

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-18 DOI: 10.1093/hr/uhaf048

Rong Xu, Chenyu Shao, Yuqi Luo, Biao Zhou, Qian Zhu, Shuqi Qiu, Zhonghua Liu, Shuoqian Liu, Chengwen Shen

{"title":"Tea polyphenol mediated CsMYB77 regulation of CsPOD44 to promote tea plant (Camellia sinensis) root drought resistance","authors":"Rong Xu, Chenyu Shao, Yuqi Luo, Biao Zhou, Qian Zhu, Shuqi Qiu, Zhonghua Liu, Shuoqian Liu, Chengwen Shen","doi":"10.1093/hr/uhaf048","DOIUrl":"https://doi.org/10.1093/hr/uhaf048","url":null,"abstract":"Drought stress significantly alters the metabolic homeostasis of tea plants; however, few studies have examined the role of specific metabolites, particularly tea polyphenols, in drought resistance. This study reveals that the tea polyphenol content in drought-tolerant tea cultivars tends to increase under drought conditions. Notably, in environments characterized by staged and repeated drought, changes in tea polyphenol are significantly positively correlated with drought resistance. To investigate this further, we irrigated the roots with exogenous tea polyphenols before subjecting the plants to drought. Our findings indicated that the absorptive roots of the experimental group exhibited enhanced development, improved cellular integrity, and a significant increase in peroxidase activity. A comprehensive analysis of the transcriptome and metabolome revealed that tea polyphenols are closely associated with the phenylpropanoid metabolism pathway. Notably, CsMYB77 and CsPOD44 genes were identified as highly correlated with this pathway. Overexpression experiments in Arabidopsis thaliana demonstrated that CsMYB77 promotes the expression of phenylpropanoid pathway genes, thereby enhancing drought resistance. Conversely, antisense oligonucleotide silencing of CsMYB77 decreased drought resistance in tea plants. Additional experiments, including yeast one-hybrid assays, luciferase complementation imaging, dual-luciferase assays, and electrophoretic mobility shift assays, confirmed that CsMYB77 positively regulates the expression of CsPOD44. In summary, our findings indicate that the differences in drought tolerance among tea cultivars are closely linked to phenylpropanoid metabolism. Specifically, tea polyphenols may mediate the regulatory network involving CsMYB77 and CsPOD44, thereby enhancing stress resistance by promoting root development. This study offers new insights into the breeding of drought-resistant tea cultivars.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"13 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The UDP-glycosyltransferase PpUGT74F2 is involved in fruit immunity via modulating salicylic acid metabolism

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-18 DOI: 10.1093/hr/uhaf049

Dan Jiang, Siyin Lin, Linfeng Xie, Miaojing Chen, Yanna Shi, Kunsong Chen, Xian Li, Boping Wu, Bo Zhang

{"title":"The UDP-glycosyltransferase PpUGT74F2 is involved in fruit immunity via modulating salicylic acid metabolism","authors":"Dan Jiang, Siyin Lin, Linfeng Xie, Miaojing Chen, Yanna Shi, Kunsong Chen, Xian Li, Boping Wu, Bo Zhang","doi":"10.1093/hr/uhaf049","DOIUrl":"https://doi.org/10.1093/hr/uhaf049","url":null,"abstract":"Flesh fruits are essential for human health, but pathogen infection poses a threat to fruit production and postharvest storage. The hormone salicylic acid (SA) and its metabolites, such as sugar conjugates and methyl salicylate MeSA, play a crucial role in regulating plant immune responses. However, the UDP-glycosyltransferases (UGTs) responsible for modulating SA metabolism in fruit have yet to be identified, and further investigation is needed to elucidate its involvement in fruit immune response. Here, we identified PpUGT74F2 as an enzyme with the highest transcription level in peach fruit, responsible for catalyzing the biosynthesis of SA glucoside (SAG), but not for MeSAG formation in fruit. Furthermore, infection of peach fruit with Monilinia fructicola, which causes brown rot disease, led to reduced expression of PpUGT74F2 resulting in significant decrease in SAG content and an increase in MeSA levels. Transgenic tomatoes expressing heterologous PpUGT74F2 increased susceptibility to gray mold. Interestingly, overexpressing PpUGT74F2 did not affect SA levels but dramatically reduced MeSA levels in response to pathogen infection, accompanied by significantly reduced expression of pathogen related (PR) genes in transgenic tomatoes. This study highlights that PpUGT74F2 acts as a negative regulatory factor for fruit immunity through a distinct mechanism not previously reported in plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"14 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into the domestication and genetic basis of yield in papaya

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-17 DOI: 10.1093/hr/uhaf045

Min Yang, Xiangdong Kong, Chenping Zhou, Ruibin Kuang, Xiaming Wu, Chuanhe Liu, Han He, Ze Xu, Yuerong Wei

{"title":"Genomic insights into the domestication and genetic basis of yield in papaya","authors":"Min Yang, Xiangdong Kong, Chenping Zhou, Ruibin Kuang, Xiaming Wu, Chuanhe Liu, Han He, Ze Xu, Yuerong Wei","doi":"10.1093/hr/uhaf045","DOIUrl":"https://doi.org/10.1093/hr/uhaf045","url":null,"abstract":"Papaya (Carica papaya L.) is an important tropical and subtropical fruit crop, and understanding its genome is essential for breeding. In this study, we assembled a high-quality genome of 344.17 Mb for the newly cultivated papaya ‘Zihui,’ which contains 22 250 protein-coding genes. By integrating 201 resequenced papaya genomes, we identified four distinct papaya groups and a 34 Mb genomic region with strong domestication selection signals. Within these regions, two key genes associated with papaya yield were discovered: Cp_zihui06549, encoding a leucine-rich receptor-like protein kinase, and Cp_zihui06768, encoding the accumulation of photosystem one 1 (APO1) protein. Heterologous expression of Cp_zihui06549 in tomato confirmed that the total number of fruits in transgenic lines more than doubled compared to wild-type plants, resulting in a significant yield increase. Furthermore, we constructed a pan-genome of papaya and obtained a 77.41 Mb non-reference sequence containing 1543 genes. Within this pan-genome, 2483 variable genes we detected, including four genes annotated as the ‘terpene synthase activity’ Gene Ontology term, which were lost in cultivars during domestication. Finally, gene retention analyses were performed using gene presence and absence variation (PAV) data and differentially expressed genes across various tissues and organs. This study provides valuable insights into the genes and loci associated with phenotypes and domestication processes, laying a solid foundation for future papaya breeding efforts.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"13 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143435153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chromosome-scale genome assembly and Epigenomic profiling reveal temperature-dependent histone methylation in iridoid biosynthesis regulation in Scrophularia ningpoensis

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-17 DOI: 10.1093/hr/uhae328

Qing Xu, Chang Liu, Bin Li, Kewei Tian, Lei You, Li Xie, Huang Wang, Meide Zhang, Wuxian Zhou, Yonghong Zhang, Chao Zhou

{"title":"A chromosome-scale genome assembly and Epigenomic profiling reveal temperature-dependent histone methylation in iridoid biosynthesis regulation in Scrophularia ningpoensis","authors":"Qing Xu, Chang Liu, Bin Li, Kewei Tian, Lei You, Li Xie, Huang Wang, Meide Zhang, Wuxian Zhou, Yonghong Zhang, Chao Zhou","doi":"10.1093/hr/uhae328","DOIUrl":"https://doi.org/10.1093/hr/uhae328","url":null,"abstract":"Understanding how medicinal plants adapt to global warming, particularly through epigenetic mechanisms that modify phenotypes without changing DNA sequences is crucial. Scrophularia ningpoensis Hemsl., a traditional Chinese Medicine (TCM), produces bioactive compounds that are influenced by environmental temperatures, making it an ideal model for studying the biological basis of TCM geoherbalism. However, the adaptive potential of epigenetic marks in S. ningpoensis under varying temperatures remains understudied, partly due to the absence of a reference genome. Here, it was demonstrated that mild warm temperatures contribute to the metabolic accumulation and the cultivated migration of S. ningpoensis using a global dataset. A high-quality chromosome-level genome was assembled, and an atlas of epigenetic, metabolic, and transcriptomic profiles across different tissues. Transcriptome analysis identified 3,401 allele-specific expressed genes (ASEGs) across nine tissues by comparing two haplotypes. ChIP-seq and BS-seq data from leaf and root tissues revealed that ASEGs are associated with distinct epigenetic patterns, particularly the active mark H3K36me3, which functions differently in these tissues. Notably, genes marked with H3K36me3 in iridoid synthesis pathway predominantly expressed in roots. Additionally, the histone methyltransferase SnSDG8 was identified to regulate ectopic H3K36me3 in iridoid biosynthesis in response to warming temperatures. Our results highlight the epigenetic mechanisms of global warming on herb-derived products, significant for medicinal plant breeding under temperature stress.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"88 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143435208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sonic-induced cellular vibrations unzip intertwined anther cone trichomes to trigger floral self-pollination and boost tomato fruit size

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-14 DOI: 10.1093/hr/uhaf053

Sidra Anwar, Happy Happy, Angus Dingley, Thailammai Vinoth, Weiguang Liang, Brian M Sindel, Laurel George, Chun H Wang, Christopher I Cazzonelli

{"title":"Sonic-induced cellular vibrations unzip intertwined anther cone trichomes to trigger floral self-pollination and boost tomato fruit size","authors":"Sidra Anwar, Happy Happy, Angus Dingley, Thailammai Vinoth, Weiguang Liang, Brian M Sindel, Laurel George, Chun H Wang, Christopher I Cazzonelli","doi":"10.1093/hr/uhaf053","DOIUrl":"https://doi.org/10.1093/hr/uhaf053","url":null,"abstract":"Summary Artificial tomato pollination methods rely on cellular vibrations from air displacement, electric vibration wands and trellis tapping, which have potential to spread pathogens. Bioacoustic frequencies emitted from buzzing bees to ultrasonication can vibrate plant cells without physical contact. The effects of frequency-dependent sonication on the poricidal anther cone sheath, self-pollination, seed set, and fruit size remain unclear. We engineered devices to investigate the frequency-dependent power-law behaviour of floral living cells from greenhouse-grown tomato varieties - contrasting contact-induced oscillations from a vibrating wand and mechanical shaker arm with precision non-contact sonication emitted by a subwoofer speaker. The velocity and acceleration of vibrating flowers and impact on poricidal anther cone sheath cellular structures, self-pollination, and fruit development were assessed. Sonic frequencies ranging from 50 to 10 000 Hz increased pollination, fruit size, weight, and seed set in Sweetelle, Endeavour, Paulanca and Managua commercial varieties. Scanning electron microscopy revealed sonication separated the intertwined trichomes and unzipped their meshed network that locks the lobes of the anther cone sheath together thereby releasing pollen grains. Near ultra-sonic frequencies boosted fruit size, whereas seed set remained constant thereby challenging the floral cell power-law rheological characteristics under different frequency scales. Tomato flowers displayed a low power-law cell behaviour to frequency-dependent sonication enabling its effectiveness as a precision non-contact technology to boost pollination and tomato fruit size without a substrate-borne component.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"13 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal dynamics and tissue-specific variations of the blueberry phyllosphere mycobiome

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-12 DOI: 10.1093/hr/uhaf042

Shay Lychen Szymanski, Timothy David Miles

{"title":"Temporal dynamics and tissue-specific variations of the blueberry phyllosphere mycobiome","authors":"Shay Lychen Szymanski, Timothy David Miles","doi":"10.1093/hr/uhaf042","DOIUrl":"https://doi.org/10.1093/hr/uhaf042","url":null,"abstract":"Highbush blueberry (Vaccinium corymbosum) is an economically important fruit-bearing woody perennial. Despite the importance of microbial communities to plant health, the above-ground (phyllosphere) microbiome of blueberry is understudied. The phyllosphere is exposed to varying conditions throughout a growing season. The fruit undergoes extensive physiological change across a season from bud to fruit. This study aimed to provide a temporal characterization of the blueberry phyllosphere across a growing season and a characterization of specific tissues and phenological stages. Blueberry branches were harvested every other week across two years and two locations during the development process of the blueberry fruits. The internal transcribed spacer regions were amplified from DNA extracts and sequenced to perform amplicon-based characterization of the fungal microbiome across time and plant tissue. Fungal communities showed changes in α-diversity depending on the week of harvest and tissue type. Early in the season, α-diversity was high, but it decreased in midseason when flowers developed into fruit. Later in the season, as the fruit ripened, α-diversity increased again. The β-diversity of the community changed across time and tissue types during plant development. Notable members of the identified core microbiome were members of the genus Alternaria, Peltaster, and Taphrina, as well as the pathogenic taxa Aureobasidium pullulans and Botrytis cinerea. This research provides background for future experimentation of understanding the microbial composition in the blueberry phyllosphere in relation to the infection court of pathogens (e.g., Colletotrichum fioriniae and Botrytis cinerea) and the temporal components of blueberry plant health and management.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"15 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaping the future of bananas: advancing genetic trait regulation and breeding in the post-genomics era

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-12 DOI: 10.1093/hr/uhaf044

Hongxia Miao, Jianbin Zhang, Yunke Zheng, Caihong Jia, Yulin Hu, Jingyi Wang, Jing Zhang, Peiguang Sun, Zhiqiang Jin, Yongfeng Zhou, Sijun Zheng, Wei Wang, Mathieu Rouard, Jianghui Xie, Juhua Liu

{"title":"Shaping the future of bananas: advancing genetic trait regulation and breeding in the post-genomics era","authors":"Hongxia Miao, Jianbin Zhang, Yunke Zheng, Caihong Jia, Yulin Hu, Jingyi Wang, Jing Zhang, Peiguang Sun, Zhiqiang Jin, Yongfeng Zhou, Sijun Zheng, Wei Wang, Mathieu Rouard, Jianghui Xie, Juhua Liu","doi":"10.1093/hr/uhaf044","DOIUrl":"https://doi.org/10.1093/hr/uhaf044","url":null,"abstract":"(Musa spp.) are among the top-produced food crops, serving as a primary source of food for millions of people. Cultivated bananas originated primarily from the wild diploid species Musa acuminata (A genome) and Musa balbisiana (B genome) through intra- and inter-specific hybridization and selections via somatic variation. Following the publication of complete A- and B-genome sequences, prospects for complementary studies on S- and T-genome traits, key gene identification for yield, ripening, quality, and stress resistance, and advances in molecular breeding have significantly expanded. In this review, latest research progress on banana A, B, S, and T genomes is briefly summarized, highlighting key advances in banana cytoplasmic inheritance, flower and fruit development, sterility, and parthenocarpy, postharvest ripening and quality regulation, and biotic and abiotic stress resistance associated with desirable economic traits. We provide updates on transgenic, gene editing, and molecular breeding. We also explore future directions for banana breeding and genetic improvement.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"50 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporally transcriptomic analyses of floral buds reveal the high-resolution landscape of flower development and dormancy regulation in peach 花蕾时空转录组分析揭示了桃花发育和休眠调控的高分辨率图谱

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-11 DOI: 10.1093/hr/uhaf029

Ya-lin Zhao, Yong Li, Dan-dan Guo, Xue-jia Chen, Ke Cao, Jin-long Wu, Wei-chao Fang, Chang-wen Chen, Xin-wei Wang, Li-rong Wang

{"title":"Spatiotemporally transcriptomic analyses of floral buds reveal the high-resolution landscape of flower development and dormancy regulation in peach","authors":"Ya-lin Zhao, Yong Li, Dan-dan Guo, Xue-jia Chen, Ke Cao, Jin-long Wu, Wei-chao Fang, Chang-wen Chen, Xin-wei Wang, Li-rong Wang","doi":"10.1093/hr/uhaf029","DOIUrl":"https://doi.org/10.1093/hr/uhaf029","url":null,"abstract":"The spatiotemporal transcriptome dataset reported here provides the peach flower bud’s gene expression atlas at spatiotemporal resolution level by 10x genomics Visium platform. This dataset can be used to define the transcript accumulation for any interesting genes across several flower bud cells by three peach flower bud samples during the activity-dormancy period, providing valuable insight into gene expression profiling and developmental stages under different environmental contexts or conditions. Importantly, we found that different cell types are related to specific regulatory programs, including signal transduction, environment and stress responses, and flower development. Our research provides insight into the transcriptomic landscape of the key cell types for flower buds and opens new avenues to study cell-type specification, function, and differentiation in Rosaceae fruit trees. A series of pivotal genes (e.g., AMS, MS188, MS1) for flower bud development were identified, these works provided a valuable reference for activity-dormancy transition in perennial deciduous fruit trees.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"14 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-silencing of PhENO1 and PhPPT alters anthocyanin production by reducing phosphoenolpyruvate supply in petunia flower

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-11 DOI: 10.1093/hr/uhaf040

Xin Li, Jiahao Cao, Guiyun Jiang, Wenqi Deng, Huimin Deng, Weiyuan Yang, Yixun Yu, Juanxu Liu

{"title":"Co-silencing of PhENO1 and PhPPT alters anthocyanin production by reducing phosphoenolpyruvate supply in petunia flower","authors":"Xin Li, Jiahao Cao, Guiyun Jiang, Wenqi Deng, Huimin Deng, Weiyuan Yang, Yixun Yu, Juanxu Liu","doi":"10.1093/hr/uhaf040","DOIUrl":"https://doi.org/10.1093/hr/uhaf040","url":null,"abstract":"The shikimate pathway is crucial for the production of aromatic amino acids and various secondary plant products, including anthocyanins. Phosphoenolpyruvate (PEP) is an important source for shikimate production. The pre-chorismate part of the shikimate pathway is confined to plastids. There are three sources of PEP in plastids. PEP can be imported into the plastids from cytoplasm via the PEP/phosphate translocator (PPT), and it can also be generated in plastids via enolase (ENO) and pyruvate orthophosphate dikinase (PPDK) catalysis. A large number of anthocyanins are synthesized in the flowers of most ornamental plants in the coloring stage. However, the source of PEP, the precursor of anthocyanin synthesis, is still unknown. Herein, Petunia hybrida PhENO1, PhPPT and PhPPDK genes were identified and their subcellular localization and expression patterns were analyzed. Silencing PhENO1, PhPPT and PhPPDK alone and co-silencing of PhENO1 and PhPPDK or PhPPT and PhPPDK did not exhibit any visible phenotypic change compared with the control, while co-silencing of PhENO1 and PhPPT resulted in the flower color change from purple to light purple. The content of PEP, shikimate, flavonoids, anthocyanins and aromatic amino acids were all significantly decreased in PhENO1 and PhPPT co-silenced plants. Co-silencing PhENO1 and PhPPT did not affect the expression level of key genes in anthocyanin synthesis and shikimate pathways. Furthermore, co-silencing of PhENO1, PhPPT and PhPPDK resulted in a phenotype similar to the co-silencing of PhENO1 and PhPPT. Altogether, our study suggested that PEP used for anthocyanin synthesis is mainly provided by PhENO1 and PhPPT, rather than PhPPDK.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"6 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Floral scent emission of Epiphyllum oxypetalum: discovery of its cytosol-localized geraniol biosynthesis

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-11 DOI: 10.1093/hr/uhaf039

Yiyang Zhang, Yuhan Zhang, Andong Zhang, Qiurui Tian, Bin Yang, Likun Wei, Wei Wu, Ting Zhu, Zhiwei Zhou, Jiaqi Wang, Zhibin Liu, Wei Tang, Haijun Xiao, Mingchun Liu, Tao Li, Qun Sun

{"title":"Floral scent emission of Epiphyllum oxypetalum: discovery of its cytosol-localized geraniol biosynthesis","authors":"Yiyang Zhang, Yuhan Zhang, Andong Zhang, Qiurui Tian, Bin Yang, Likun Wei, Wei Wu, Ting Zhu, Zhiwei Zhou, Jiaqi Wang, Zhibin Liu, Wei Tang, Haijun Xiao, Mingchun Liu, Tao Li, Qun Sun","doi":"10.1093/hr/uhaf039","DOIUrl":"https://doi.org/10.1093/hr/uhaf039","url":null,"abstract":"Epiphyllum oxypetalum, a renowned ornamental species in Cactaceae, releases attractive fragrance during its infrequent, transient and nocturnal blooms. However, the floral fragrance composition and biosynthesis remain largely unexplored. Employing volatilomics, transcriptomics and biochemistry, we systematically characterized the composition, emission dynamics and biosynthesis of the floral scent of E. oxypetalum. The floral scent composition of E. oxypetalum was highly dynamic. Starting after 20:00 local time, volatile emission increased 200-fold within six hours. At full bloom, geraniol accounted for 72.54% of the total emission, followed by benzyl alcohol (12.96%) and methyl salicylate (3.75%). These scents predominantly originated from petals and sepals. Transcriptomic analysis and inhibition assays using pathway-specific inhibitors revealed that the mevalonate pathway was the precursor source for geraniol biosynthesis. Functionally characterized cytosol localizing geraniol synthase EoTPSa1 was the key enzyme responsible for geraniol biosynthesis. Together, these findings pinpointed a cytosolic biosynthetic route for the major scent volatile geraniol in E. oxypetalum. Our study provides new insights into the emission dynamics and biosynthesis of E. oxypetalum floral scents. In particular, we demonstrated a distinctive mevalonate pathway-based geraniol biosynthetic pathway, which may hold potential for the development of novel perfume products.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"129 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0