Horticulture Research最新文献_第2页

Aluminum-activated malate transporter family member CsALMT6 mediates fluoride resistance in tea plants (Camellia sinensis) 铝激活的苹果酸转运体家族成员CsALMT6介导茶树（Camellia sinensis）的氟抗性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-12 DOI: 10.1093/hr/uhae353

Qinghui Li, Ruiming Zhang, Xinlong Hu, Dejiang Ni, Yuqiong Chen, Mingle Wang

{"title":"Aluminum-activated malate transporter family member CsALMT6 mediates fluoride resistance in tea plants (Camellia sinensis)","authors":"Qinghui Li, Ruiming Zhang, Xinlong Hu, Dejiang Ni, Yuqiong Chen, Mingle Wang","doi":"10.1093/hr/uhae353","DOIUrl":"https://doi.org/10.1093/hr/uhae353","url":null,"abstract":"Tea plant is a fluoride (F)-hyperaccumulator, which poses a potential threat to human health via tea consumption. Reducing F accumulation in fresh tea leaves is crucial for enhancing the safety of tea production at its source. This study aims to isolate novel genes responsible for F accumulation or transport in tea plants. We identified an aluminum (Al)-activated malate transporter gene, CsALMT6, which was hypothesized to be a candidate for differential F accumulation in Camellia sinensis, by employing a combination of transcriptome-wide association study (TWAS) and genome-wide identification of the CsALMT gene family. CsALMT6 exhibited high expression levels in old leaves, and its expression was significantly upregulated in tea plants subjected to F-stress conditions. Furthermore, heterologous expression of CsALMT6 in yeast, Arabidopsis, and Populus conferred F tolerance. However, the expression of F-tolerant hub genes, CsFEX1 and CsFEX2, remained unaffected in CsALMT6-silenced tea plants. Additionally, under F toxicity conditions, the transcription of CsALMT6 was negatively associated with F accumulation in tea plants. In conclusion, CsALMT6 plays a vital role in reducing F accumulation in C. sinensis, thus conferring F tolerance to plant cells.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"1 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Strategies for Paclitaxel Production: Insights from Plant Cell Culture, Endophytic Microorganisms, and Synthetic Biology

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-12 DOI: 10.1093/hr/uhae346

Jia-Yuan Yin, Meng Lai, Xiao-Ying Yu, Ding-Ding Su, Xing-Yao Xiong, Yan-Lin Li

引用次数: 0

Molecular Insights into TT2-Type MYB Regulators Illuminate the Complexity of Floral Flavonoids Biosynthesis in Freesia hybrida

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-12 DOI: 10.1093/hr/uhae352

Xiaotong Shan, Deyu Zhuang, Ruifang Gao, Meng Qiu, Liudi Zhou, Jia Zhang, Yanan Wang, Qi Zhang, Niu Zhai, Guoyun Xu, Li Wang, Yueqing Li, Xiang Gao

{"title":"Molecular Insights into TT2-Type MYB Regulators Illuminate the Complexity of Floral Flavonoids Biosynthesis in Freesia hybrida","authors":"Xiaotong Shan, Deyu Zhuang, Ruifang Gao, Meng Qiu, Liudi Zhou, Jia Zhang, Yanan Wang, Qi Zhang, Niu Zhai, Guoyun Xu, Li Wang, Yueqing Li, Xiang Gao","doi":"10.1093/hr/uhae352","DOIUrl":"https://doi.org/10.1093/hr/uhae352","url":null,"abstract":"Proanthocyanidins (PAs), anthocyanins, and flavonols are key flavonoids that play diverse roles in plant physiology and human health. Despite originating from a shared biosynthetic pathway, the regulatory mechanisms of PA biosynthesis and the cooperative regulation of different kinds of flavonoids remain elusive, particularly in flower tissues or organs. Here, we elucidated the regulatory network governing PA biosynthesis in Freesia hybrida ‘Red River®’ by characterizing four TT2-type MYB transcription factors, designated FhMYBPAs. Phylogenetic analysis, subcellular localization, and transactivation assays predicted their roles as PA-related activators. Pearson correlation analysis revealed significant correlations between FhMYBPAs and PA accumulation in various floral tissues and development stages. Functional studies demonstrated that FhMYBPAs activated PA biosynthesis by directly binding to the promoters of target genes, which can be enhanced by FhTT8L. Additionally, a hierarchical and feedback regulatory model involving FhTTG1, FhMYB27, and FhMYBx was proposed for PA biosynthesis. Furthermore, comparative analysis of flavonoid-related MYB factors involving FhPAP1, FhMYB5, FhMYBF1 and FhMYB21L2 highlighted their roles in regulating PA, anthocyanin, and flavonol biosynthesis, with some exhibiting versatile regulations. Overall, our findings provide insights into the spatio-temporal regulation of flavonoids in flowers and expand our understanding of MYB-mediated transcriptional regulation of specialized metabolites in plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"30 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor ClWRKY61 interacts with ClLEA55 to enhance salt tolerance in watermelon

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-11 DOI: 10.1093/hr/uhae320

Guangpu Lan, Changqing Xuan, Yidong Guo, Xin Huang, Mengjiao Feng, Li Yuan, Hao Li, Jianxiang Ma, Yong Zhang, Zhongyuan Wang, Jianqiang Yang, Rong Yu, Feishi Luan, Xian Zhang, Chunhua Wei

{"title":"The transcription factor ClWRKY61 interacts with ClLEA55 to enhance salt tolerance in watermelon","authors":"Guangpu Lan, Changqing Xuan, Yidong Guo, Xin Huang, Mengjiao Feng, Li Yuan, Hao Li, Jianxiang Ma, Yong Zhang, Zhongyuan Wang, Jianqiang Yang, Rong Yu, Feishi Luan, Xian Zhang, Chunhua Wei","doi":"10.1093/hr/uhae320","DOIUrl":"https://doi.org/10.1093/hr/uhae320","url":null,"abstract":"High salinity can severely inhibit the growth and development of watermelon (Citrullus lanatus L.). WRKY proteins are believed to mediate the adaptation of plants to abiotic stresses. Here, we identified the ClWRKY61 gene, which positively regulates the tolerance of watermelon to salt stress. Knockout of the ClWRKY61 reduced salt tolerance, while overexpression of the ClWRKY61 enhanced salt tolerance in watermelon according to phenotypic and physiological analyses. Yeast two-hybrid assays revealed that ClWRKY61 interacts with the ClLEA55 protein, and this interaction was further confirmed by luciferase complementation imaging, transient bimolecular fluorescence complementation, and GST pull-down assays. Knockout of the ClLEA55 resulted in lower salt tolerance compared to the wild-type plants. RNA-seq analysis indicated 421 up-regulated and 133 down-regulated genes in the ClWRKY61 knockout line under salt stress, containing 293 differentially expressed genes with W-box in their promoters. After salt treatment of watermelon seedlings, qRT-PCR assays evidenced that the expression of genes encoding phytoene synthase, MYB transcription factor, sucrose synthase, alpha/beta-hydrolases superfamily protein, glutathione reductase, and sugar transporter were significantly increased; while the expression of genes encoding LEA protein, WRKY transcription factor, ERF transcription factor, alpha-glucan water dikinase, and calcium-dependent protein kinase were significantly decreased in ClWRKY61 knockout lines. These results provide an opportunity to mediate the regulation of salt stress in watermelon with WRKY proteins.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"28 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the Study of Senescence Mechanisms in the Genus Paeonia

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-10 DOI: 10.1093/hr/uhae344

Yuxuan Wang, Miao Sun, Wei Zhu, Le Chen, Shaocai Zhu, Jiageng Zhao, Jaime A Teixeira da Silva, Xiaonan Yu

{"title":"Advances in the Study of Senescence Mechanisms in the Genus Paeonia","authors":"Yuxuan Wang, Miao Sun, Wei Zhu, Le Chen, Shaocai Zhu, Jiageng Zhao, Jaime A Teixeira da Silva, Xiaonan Yu","doi":"10.1093/hr/uhae344","DOIUrl":"https://doi.org/10.1093/hr/uhae344","url":null,"abstract":"Tree and herbaceous peony are considerably important ornamental plants within the genus Paeonia, and hold substantial horticultural value. This review summarizes the progress in research on the senescence mechanisms of tree and herbaceous peony flowers, focusing on the regulation of gene expression, hormonal interactions, and the influence of environmental factors on senescence. Using high-throughput sequencing technologies, key genes displaying differential expression during senescence have been identified, and these play central roles in hormone signaling and cellular senescence. The interactions among plant hormones, including ethylene, abscisic acid, gibberellins, cytokinins, and auxins, also play key roles in the regulation of senescence. Adjustments in antioxidant levels, as well as water and energy metabolism, are critical factors in the delay of senescence. Environmental factors, including light, temperature, drought, and salt stress, also significantly affect senescence. Additionally, this review proposes future research directions, including the expansion of the molecular regulatory network of senescence in Paeonia, the use of gene editing technologies like CRISPR/Cas9, multi-omics studies, and exploratory comparative research on spatial biology senescence mechanisms. These studies aim to deepen our understanding of the molecular mechanisms that underlie senescence in Paeonia, and provide a scientific basis for cultivar improvement and postharvest management of these ornamental commodities in the horticultural industry.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"141 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mining the cucumber core collection: Phenotypic and genetic characterization of morphological diversity for fruit quality characteristics

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-10 DOI: 10.1093/hr/uhae340

Ying-Chen Lin, Yiqun Weng, Zhangjun Fei, Rebecca Grumet

{"title":"Mining the cucumber core collection: Phenotypic and genetic characterization of morphological diversity for fruit quality characteristics","authors":"Ying-Chen Lin, Yiqun Weng, Zhangjun Fei, Rebecca Grumet","doi":"10.1093/hr/uhae340","DOIUrl":"https://doi.org/10.1093/hr/uhae340","url":null,"abstract":"Commercial value of cucumber is primarily driven by fruit quality. However, breeding goals frequently focus on production constraints caused by biotic and abiotic stresses. As sources of resistances are often present in unadapted germplasm, we sought to provide morphological and genetic information characterizing the diversity of fruit quality traits present in the CucCAP cucumber core collection. These 388 accessions representing &gt;96% of the genetic diversity for cucumber present in the U.S. National Plant Germplasm System harbor important sources of resistances and extensive morphological diversity. Data were collected for skin color, length/diameter ratio (L/D), tapering, curvature, and spine density for young fruits [5-7 days post-pollination (dpp)], and length, diameter, L/D, skin color, netting, seed cavity size, flesh thickness, hollowness, and flesh color for mature fruits (30-40 dpp. Significant associations of SNPs with each trait were identified from genome-wide association studies (GWAS). In several cases, QTL for highly correlated traits were closely clustered. Principal component analysis, driven primarily by the highly correlated traits of fruit length, young and mature L/D ratios, and curvature showed a clear divergence of East Asian accessions. Significant SNPs contributing to the longest fruits, including development-stage specific QTL, were distributed across multiple chromosomes, indicating broad genomic effects of selection. Many of the SNPs identified for the various morphological traits were in close vicinity to previously identified fruit trait QTL and candidate genes, while several novel genes potentially important for these traits were also identified.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"19 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chromosome-level reference genome facilitates the discovery of clubroot resistant gene Crr5 in Chinese cabbage

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-10 DOI: 10.1093/hr/uhae338

Shuangjuan Yang, Xiangfeng Wang, Zhaojun Wang, Wenjing Zhang, Henan Su, Xiaochun Wei, Yanyan Zhao, Zhiyong Wang, Xiaowei Zhang, Li Guo, Yuxiang Yuan

{"title":"A chromosome-level reference genome facilitates the discovery of clubroot resistant gene Crr5 in Chinese cabbage","authors":"Shuangjuan Yang, Xiangfeng Wang, Zhaojun Wang, Wenjing Zhang, Henan Su, Xiaochun Wei, Yanyan Zhao, Zhiyong Wang, Xiaowei Zhang, Li Guo, Yuxiang Yuan","doi":"10.1093/hr/uhae338","DOIUrl":"https://doi.org/10.1093/hr/uhae338","url":null,"abstract":"Brassica rapa includes a variety of important vegetable and oilseed crops, yet it is significantly challenged by clubroot disease. Notably, the majority of genotypes of B. rapa with published genomes exhibit high susceptibility to clubroot disease. The present study presents a high-quality chromosome-level sequence of the genome of the DH40 clubroot-resistant (CR) line, a doubled haploid line derived from the hybrid progeny of a European turnip (ECD01) and two lines of Chinese cabbage. The assembled genome spans 420.92 Mb, with a contig N50 size of 11.97 Mb. Comparative genomics studies revealed that the DH40 line is more closely related to the Chinese cabbage Chiifu than to the turnip ECD04. The DH40 genome provided direct reference and greatly facilitate the map-based cloning of the clubroot resistance gene Crr5, encoding a nucleotide-binding leucine-rich repeat (NLR) protein. Further functional analysis demonstrated that Crr5 confers clubroot resistance in both Chinese cabbage and transgenic Arabidopsis. It responds to inoculation with Plasmodiophora brassicae and is expressed in both roots and leaves. Subcellular localization shows that Crr5 is present in the nucleus. Notably, the TIR domain of Crr5 can autoactivate and trigger cell death. In addition, we developed two Crr5-specific KASP markers and showcased their successful application in breeding CR Chinese cabbage through marker-assisted selection. Overall, our research offers valuable resources for genetic and genomic studies in B. rapa and deepens our understanding of the molecular mechanisms underlying clubroot resistance against Plasmodiophora brassicae.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"40 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LoBLH6 interacts with LoMYB65 to regulate anther development through feedback regulation of gibberellin synthesis in lily

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-10 DOI: 10.1093/hr/uhae339

Junpeng Yu, Ze Wu, Xinyue Liu, Qianqian Fang, Xue Pan, Sujuan Xu, Man He, Jinxing Lin, Nianjun Teng

{"title":"LoBLH6 interacts with LoMYB65 to regulate anther development through feedback regulation of gibberellin synthesis in lily","authors":"Junpeng Yu, Ze Wu, Xinyue Liu, Qianqian Fang, Xue Pan, Sujuan Xu, Man He, Jinxing Lin, Nianjun Teng","doi":"10.1093/hr/uhae339","DOIUrl":"https://doi.org/10.1093/hr/uhae339","url":null,"abstract":"The homeostasis of gibberellin (GA) is crucial for the normal development of anthers, but its underlying regulatory mechanisms are not clear. The GA induced v-Myb myeloblastosis viral oncogene homolog (MYB) transcription factor LoMYB65 is involved in anther development. In this study, we screened and identified an interacting protein of LoMYB65, Lilium Oriental Hybrids BEL1-Like Homeodomain6 (LoBLH6). LoBLH6 was localized in both the nucleus and cytoplasm, and it interacted with LoMYB65 through its BELL domain, exhibiting transcriptional repression activity. LoBLH6 was continuously expressed during anther development, with particularly high expression in the mid and late stages. In situ hybridization revealed high expression of LoBLH6 in the tapetum and microspores, with the same tissue specificity as LoMYB65. Silencing of LoBLH6 in lilies resulted in abnormal anther development, reduced pollen, and increased GA content. The application of GA induced phenotypes in the anthers and pollen of lily that were similar to the silencing of LoBLH6. Further research showed that LoBLH6 directly binds to the promoter of Lilium Oriental Hybrids GA 20-oxidase1 (LoGA20ox1) to suppress its expression, and co-expression with LoMYB65 enhances this repression. Additionally, GA treatment enhanced the interaction between LoBLH6 and LoMYB65 and their complex's inhibitory effect on downstream target genes. During the transition from microspores to mature pollen grains in lily anthers, GA levels maintain a steady state, which is disrupted by silencing LoBLH6, leading to abnormal pollen development. Overall, our results reveal that the interaction between LoBLH6 and LoMYB65 regulates anther development through feedback regulation of GA synthesis.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"154 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deactivating mutations in the catalytic site of a companion serine carboxypeptidase-like acyltransferase enhance catechin galloylation in Camellia plants

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-12-10 DOI: 10.1093/hr/uhae343

Xiangxiang Chen, Xue Zhang, Yue Zhao, Liping Gao, Zhihui Wang, Yanlei Su, Lingyun Zhang, Tao Xia, Yajun Liu

{"title":"Deactivating mutations in the catalytic site of a companion serine carboxypeptidase-like acyltransferase enhance catechin galloylation in Camellia plants","authors":"Xiangxiang Chen, Xue Zhang, Yue Zhao, Liping Gao, Zhihui Wang, Yanlei Su, Lingyun Zhang, Tao Xia, Yajun Liu","doi":"10.1093/hr/uhae343","DOIUrl":"https://doi.org/10.1093/hr/uhae343","url":null,"abstract":"Galloylated flavan-3-ols are key quality and health-related compounds in tea plants of Camellia section Thea. Camellia ptilophylla and Camellia sinensis are two representative species known for their high levels of galloylated flavan-3-ols. Building on our knowledge of galloyl catechin biosynthesis in C. sinensis, we now focus on the biosynthesis of galloylated phenolics in C. ptilophylla, aiming to elucidate the mechanisms underlying the high accumulation of these compounds in Camellia species. The phenolic compounds in C. ptilophylla were identified and quantified using chromatographic and colorimetric methods. Genes involved in polyphenol galloylation were identified by correlating gene expression with the accumulation of galloylated phenolics across 18 additional Camellia species and 1 related species using Weighted Gene Co-expression Network Analysis. Key findings include the co-expression of SCPL4/2 and SCPL5 subgroup enzymes as crucial for galloylation of catechins, while SCPL3 and SCPL8 showed enzymatic activity related to hydrolyzable tannin synthesis. Variations in the amino acid sequences of SCPL5, particularly in the catalytic triad (T-D-Y vs. S-D-H) observed in C. ptilophylla and C. sinensis, were found to significantly affect enzymatic activity and EGCG production. In conclusion, this research provides important insights into the metabolic pathways of C. ptilophylla, emphasizing the critical role of SCPL enzymes in shaping the phenolic profile within the section Thea. The findings have significant implications for the cultivation and breeding of tea plants with optimized phenolic characteristics.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"47 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined genomic, transcriptomic, and metabolomic analyses provide insights into the fruit development of bottle gourd (Lagenaria siceraria)

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-11-27 DOI: 10.1093/hr/uhae335

Xuelian He, Yanyan Zheng, Songguang Yang, Ying Wang, Yu’e Lin, Biao Jiang, Dasen Xie, Wenrui Liu, Qingwu Peng, Jinhua Zuo, Min Wang

{"title":"Combined genomic, transcriptomic, and metabolomic analyses provide insights into the fruit development of bottle gourd (Lagenaria siceraria)","authors":"Xuelian He, Yanyan Zheng, Songguang Yang, Ying Wang, Yu’e Lin, Biao Jiang, Dasen Xie, Wenrui Liu, Qingwu Peng, Jinhua Zuo, Min Wang","doi":"10.1093/hr/uhae335","DOIUrl":"https://doi.org/10.1093/hr/uhae335","url":null,"abstract":"Bottle gourd (Lagenaria siceraria (Molina) Standl) is a widely distributed Cucurbitaceae species, but gaps and low-quality assemblies have limited its genomic study. To address this, we assembled a nearly complete, high-quality genome of the bottle gourd (Pugua) using PacBio HiFi sequencing and Hi-C correction. The genome, being 298.67 Mb long with a ContigN50 of 28.55 Mb, was identified to possess 11 chromosomes, 11 centromeres, 18 telomeres, and 24,439 predicted protein-coding genes; notably, gap-free telomere-to-telomere assembly was accomplished for seven chromosomes. Based on the Pugua genome, the transcriptomic and metabolomic combined analyses revealed that amino acids and lipids accumulate during the expansion stage, while sugars and terpenoids increase during ripening. GA4 and genes of the Aux/IAA family mediate fruit expansion and maturation, while cell wall remodeling is regulated by factors such as XTHs, EXPs, polyphenols, and alkaloids, contributing to environmental adaptation. GGAT2 was positively correlated with glutamate, a source of umami, and SUS5 and SPS4 expression aligned with sucrose accumulation. This study provides a valuable genetic resource for bottle gourd research, enhancing the understanding of Cucurbitaceae evolution and supporting further studies on bottle gourd development, quality, and genetic improvement.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"26 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142753704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0