Horticulture Research最新文献_第3页

The ubiquitin ligase VviPUB19 negatively regulates grape cold tolerance by affecting the stability of ICEs and CBFs 泛素连接酶 VviPUB19 通过影响 ICEs 和 CBFs 的稳定性来负向调节葡萄的耐寒性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-23 DOI: 10.1093/hr/uhae297

Ling Wang, Mengyu Zhao, Xue Zhang, Ting Zhao, Congbo Huang, Yujin Tang, Yan Li, Chaohong Zhang

引用次数: 0

High-quality genome of black wolfberry (Lycium ruthenicum Murr.) provides insights into the genetics of anthocyanin biosynthesis regulation 黑枸杞（Lycium ruthenicum Murr.）的高质量基因组为花青素生物合成的遗传调控提供了启示

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-23 DOI: 10.1093/hr/uhae298

Yuhui Xu, Haoxia Li, Tongwei Shi, Qing Luo, Yuchao Chen, Shenghu Guo, Weiwei Tian, Wei An, Jian Zhao, Yue Yin, Jun He, Rui Zheng, Xiaojie Liang, Yajun Wang, Xiyan Zhang, Zhigang Shi, Linyuan Duan, Xiaoya Qin, Ting Huang, Bo Zhang, Ru Wan, Yanlong Li, Youlong Cao, Hui Liu, Sheng Shu, Aisheng Xiong, Jianhua Zhao

{"title":"High-quality genome of black wolfberry (Lycium ruthenicum Murr.) provides insights into the genetics of anthocyanin biosynthesis regulation","authors":"Yuhui Xu, Haoxia Li, Tongwei Shi, Qing Luo, Yuchao Chen, Shenghu Guo, Weiwei Tian, Wei An, Jian Zhao, Yue Yin, Jun He, Rui Zheng, Xiaojie Liang, Yajun Wang, Xiyan Zhang, Zhigang Shi, Linyuan Duan, Xiaoya Qin, Ting Huang, Bo Zhang, Ru Wan, Yanlong Li, Youlong Cao, Hui Liu, Sheng Shu, Aisheng Xiong, Jianhua Zhao","doi":"10.1093/hr/uhae298","DOIUrl":"https://doi.org/10.1093/hr/uhae298","url":null,"abstract":"Black wolfberry (Lycium ruthenicum Murr.) is an important plant for ecological preservation. In addition, its fruits are rich in anthocyanins and have important edible and medicinal value. However, a high quality chromosome-level genome for this species is not yet available, and the regulatory mechanisms involved in the biosynthesis of anthocyanins are unclear. In this study, haploid material was used to assemble a high-quality chromosome-level reference genome of Lycium ruthenicum, resulting in a genome size of 2,272 Mb with contig N50 of 92.64 Mb, and 38,993 annotated gene models. In addition, the evolution of this genome and large-scale variations compared with the Ningxia wolfberry Lycium barbarum were determined. Importantly, homology annotation identified 86 genes involved in the regulatory pathway of anthocyanin biosynthesis, five of which [LrCHS1 (evm.TU.Chr05.295), LrCHS2 (evm.TU.Chr09.488), LrAOMT (evm.TU.Chr09.809), LrF3'5'H (evm.TU.Chr06.177) and LrAN2.1 (evm.TU.Chr05.2618)] were screened by differential expression analysis and correlation analysis using a combination of transcriptome and metabolome testing. Overexpression of these genes could significantly up- or downregulate anthocyanin-related metabolites. These results will help accelerate the functional genomic research of L. ruthenicum, and the elucidation of the genes involved in anthocyanin synthesis will be beneficial for breeding new varieties and further exploring its ecological conservation potential.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"25 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142488745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The GRAS transcription factor PtrPAT1 of Poncirus trifoliata functions in cold tolerance and modulates glycine betaine content by regulating the BADH-like gene 三叶椿的 GRAS 转录因子 PtrPAT1 具有耐寒功能，可通过调节 BADH 样基因调节甘氨酸甜菜碱的含量

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-23 DOI: 10.1093/hr/uhae296

Ruhong Ming, Tian Fang, Wei Ling, Jingjing Geng, Jing Qu, Yu Zhang, Jianhua Chen, Shaochang Yao, Liangbo Li, Ding Huang, Ji-Hong Liu

{"title":"The GRAS transcription factor PtrPAT1 of Poncirus trifoliata functions in cold tolerance and modulates glycine betaine content by regulating the BADH-like gene","authors":"Ruhong Ming, Tian Fang, Wei Ling, Jingjing Geng, Jing Qu, Yu Zhang, Jianhua Chen, Shaochang Yao, Liangbo Li, Ding Huang, Ji-Hong Liu","doi":"10.1093/hr/uhae296","DOIUrl":"https://doi.org/10.1093/hr/uhae296","url":null,"abstract":"GRAS, termed after GAI (gibberellic acid insensitive), RGA (repressor of GA1), and SCR (scarecrow), is a plant-specific transcription factor crucial for plant development and stress response. However, understanding of the functions played by the GRAS members and their target genes in citrus is limited. In this study, we identified a cold stress-responsive GRAS gene from Poncirus trifoliate, designated as PtrPAT1, by yeast one-hybrid library screening using the promoter of PtrBADH-l, a betaine aldehyde dehydrogenase (BADH)-like gene. PtrPAT1, belonging to the PAT1 subfamily, was localized in the nucleus and plasma membrane, exhibited transactivation activity and showed a remarkable upregulation under cold stress. Overexpression of PtrPAT1 elevated BADH activity, increased glycine betaine (GB) accumulation and conferred enhanced cold tolerance in transgenic tobacco plants compared with wild type, while downregulation in trifoliate orange by virus-induced gene silencing (VIGS) resulted in opposite trends. Furthermore, the activities of two antioxidant enzymes, including peroxidase (POD) and superoxide dismutase (SOD), were significantly increased in the overexpression plants, but remarkably decreased in the VIGS line, consistent with accumulation patterns of the reactive oxygen species (ROS). PtrPAT1 was demonstrated to interact with and activate the PtrBADH-l promoter through the putative PAT1-binding motif with the core sequence of TTTCATGT, indicating that PtrBADH-l is a target gene of PtrPAT1. Taken together, these results demonstrate that PtrPAT1 positively affects cold tolerance through the regulation of GB biosynthesis by modulating PtrBADH-l expression.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"12 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142488746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic selection and genetic architecture of agronomic traits during modern flowering Chinese cabbage breeding 现代开花大白菜育种过程中的基因组选择和农艺性状的遗传结构

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-18 DOI: 10.1093/hr/uhae299

Yahui Zhao, Guangguang Li, Zhangsheng Zhu, Ming Hu, Ding Jiang, Muxi Chen, Juantao Wang, Kexin Zhang, Yansong Zheng, Yi Liao, Changming Chen

{"title":"Genomic selection and genetic architecture of agronomic traits during modern flowering Chinese cabbage breeding","authors":"Yahui Zhao, Guangguang Li, Zhangsheng Zhu, Ming Hu, Ding Jiang, Muxi Chen, Juantao Wang, Kexin Zhang, Yansong Zheng, Yi Liao, Changming Chen","doi":"10.1093/hr/uhae299","DOIUrl":"https://doi.org/10.1093/hr/uhae299","url":null,"abstract":"Flowering Chinese cabbage is a type of leafy vegetable that belongs to the Brassica genus. Originally native to South China, it is now widely cultivated and consumed across the globe, particularly in Asian countries. The recent cultivation and regional expansion of flowering Chinese cabbage provides a valuable opportunity to elucidate the genomic basis underlying environmental adaptation and desired traits during a short-term artificial selection process. Here, we investigate the genetic variation, population structure, and diversity of a diverse germplasm collection of 403 flowering Chinese cabbage accessions. Our investigation seeks to elucidate the genomic basis that guide the selection of adaptability, yield, and pivotal agronomic traits. We further investigated breeding improvement associated with stem development by integrating transcriptome data. Genome-wide association analysis identified 642 loci and corresponding candidate genes associated with 11 essential agronomic traits, including plant architecture and yield. Furthermore, we uncovered a significant disparity in the allele frequency distribution of non-synonymous mutations in these candidate genes throughout the improvement stages. Our results shed light on the genetic basis of improvement and crucial agronomic traits in flowering Chinese cabbage, offering invaluable resources for upcoming genomics-assisted breeding endeavors.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"55 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A transcription factor, PbWRKY24, contributes to russet skin formation in pear fruits by modulating lignin accumulation 转录因子 PbWRKY24 通过调节木质素积累促进梨果赤褐色果皮的形成

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-18 DOI: 10.1093/hr/uhae300

Jialong Wang, Dong Wang, Mingrui Zhao, Mengyuan Yu, Xiaodong Zheng, Yike Tian, Zhijuan Sun, Xiaoli Liu, Caihong Wang, Changqing Ma

{"title":"A transcription factor, PbWRKY24, contributes to russet skin formation in pear fruits by modulating lignin accumulation","authors":"Jialong Wang, Dong Wang, Mingrui Zhao, Mengyuan Yu, Xiaodong Zheng, Yike Tian, Zhijuan Sun, Xiaoli Liu, Caihong Wang, Changqing Ma","doi":"10.1093/hr/uhae300","DOIUrl":"https://doi.org/10.1093/hr/uhae300","url":null,"abstract":"Skin color is one of the major traits of fruit appearance quality in pear (Pyrus), which affects the fruit commodity value. Russet skin protects pear fruits from environmental stresses and its formation process is closely linked to lignin accumulation. However, the molecular regulatory networks underlying russet skin formation in pear fruits involve complex secondary metabolic pathways and remain elusive. Here, we explored the regulatory mechanisms underlying lignin accumulation in pear skin based on transcriptome sequencing, co-expression network analysis, and gene expression profiling. We identified a WRKY transcription factor gene, PbWRKY24, that regulates russet skin formation in pear fruits. The relative expression of PbWRKY24 in russet pear skin was significantly correlated with lignin content. We then verified the function of PbWRKY24 in lignin accumulation via genetic transformation. DNA affinity purification sequencing revealed that PbWRKY24 directly binds to the promoter of a lignin biosynthesis gene, PbPRX4. This binding was confirmed by yeast one-hybrid, dual-luciferase, and electrophoretic mobility shift assays. Overexpression of PbPRX4 in pear skin stimulated lignin accumulation and consequently promoted russet skin formation. This study provides a glimpse into the intricate lignin biosynthesis mechanisms during russet skin formation in pear fruits, which is of practical significance to pear breeding for fruit quality.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"12 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fertility restorer gene CaRf and PepperSNP50K provide a promising breeding system for hybrid pepper 生育力恢复基因 CaRf 和 PepperSNP50K 为杂交辣椒提供了一个前景广阔的育种系统

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-16 DOI: 10.1093/hr/uhae223

Bingqian Tang, Huiping Yang, Qinbiao Yin, Wu Miao, Yuting Lei, Qingzhi Cui, Jiawen Cheng, Xinhao Zhang, Ying Chen, Juan Du, Lingling Xie, Shunxue Tang, Meiqi Wang, Jiayue Li, Mingyue Cao, Li Chen, Fangling Xie, Xiumin Li, Fan Zhu, Zhongyi Wang, Cheng Xiong, Xiongze Dai, Xuexiao Zou, Feng Liu

{"title":"Fertility restorer gene CaRf and PepperSNP50K provide a promising breeding system for hybrid pepper","authors":"Bingqian Tang, Huiping Yang, Qinbiao Yin, Wu Miao, Yuting Lei, Qingzhi Cui, Jiawen Cheng, Xinhao Zhang, Ying Chen, Juan Du, Lingling Xie, Shunxue Tang, Meiqi Wang, Jiayue Li, Mingyue Cao, Li Chen, Fangling Xie, Xiumin Li, Fan Zhu, Zhongyi Wang, Cheng Xiong, Xiongze Dai, Xuexiao Zou, Feng Liu","doi":"10.1093/hr/uhae223","DOIUrl":"https://doi.org/10.1093/hr/uhae223","url":null,"abstract":"Cytoplasmic male sterility (CMS) is pivotal in plant breeding and widely employed in various crop hybrids, including pepper. However, the functional validation of the restorer of fertility (Rf) gene in pepper has been lacking until now. This study identifies and characterizes CaRf, a single dominant locus crucial for restoring CMS in the pepper strong recovery inbred line Zhangshugang. The CaRf gene encodes a mitochondria-targeted pentatricopeptide repeat protein, validated through the induction of male sterility upon its silencing in hybrid F1 plants. To enhance pepper breeding efficiency, 176 important pepper breeding parent materials were resequenced, and a PepperSNP50K liquid-phase breeding chip was developed, comprising 51 172 markers. Integration of CaRf functional characterization and PepperSNP50K facilitated the development of a high-quality red pepper hybrid. These findings provide significant insights and practical strategies for advancing molecular-designed breeding in peppers.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"193 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and interactions of rhizobacteria determine multinutrient traits in tomato host plants under nitrogen and water disturbances 根瘤菌的多样性和相互作用决定了氮和水干扰下番茄寄主植物的多营养性状

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-15 DOI: 10.1093/hr/uhae290

Wenxuan Shi, Junjie Guo, Xinxuan Yu, Zhixing Li, Boyang Weng, Danxia Wang, Shihao Su, Yufei Sun, Jinfang Tan, Ruohan Xie

{"title":"Diversity and interactions of rhizobacteria determine multinutrient traits in tomato host plants under nitrogen and water disturbances","authors":"Wenxuan Shi, Junjie Guo, Xinxuan Yu, Zhixing Li, Boyang Weng, Danxia Wang, Shihao Su, Yufei Sun, Jinfang Tan, Ruohan Xie","doi":"10.1093/hr/uhae290","DOIUrl":"https://doi.org/10.1093/hr/uhae290","url":null,"abstract":"Coevolution within the plant holobiont extends the capacity of host plants for nutrient acquisition and stress resistance. However, the role of the rhizospheric microbiota in maintaining multinutrient utilization (i.e., multinutrient traits) in the host remains to be elucidated. Multinutrient cycling index (MNC), analogous to the widely used multifunctionality index, provides a straightforward and interpretable measure of the multinutrient traits in host plants. Using tomato as a model plant, we characterized MNC (based on multiple aboveground nutrient contents) in host plants under different nitrogen and water supply regimes and explored the associations between rhizospheric bacterial community assemblages and host-plant multinutrient profiles. Rhizosphere bacterial community diversity, quantitative abundance, predicted function, and key topological features of the co-occurrence network were more sensitive to water supply than to nitrogen supply. A core bacteriome comprising 61 genera, such as Candidatus Koribacter and Streptomyces, persisted across different habitats and served as a key predictor of host-plant nutrient uptake. The MNC index increased with greater diversity and higher core taxon abundance in the rhizobacterial community, while decreasing with higher average degree and graph density of rhizobacterial co-occurrence network. Multinutrient absorption by host plants was primarily regulated by community diversity and rhizobacterial network complexity under the interaction of nitrogen and water. The high biodiversity and complex species interactions of the rhizospheric bacteriome play crucial roles in host-plant performance. This study supports the development of rhizosphere microbiome engineering, facilitating effective manipulation of the microbiome for enhanced plant benefits, which supports sustainable agricultural practices and plant health.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"7 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Editing for Grass Improvement and Future Agriculture 基因组编辑促进草地改良和未来农业

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-15 DOI: 10.1093/hr/uhae293

Bilal Muhammad, Jie Geng, Lin Chen, Pedro García-Caparros, Tao Hu

{"title":"Genome Editing for Grass Improvement and Future Agriculture","authors":"Bilal Muhammad, Jie Geng, Lin Chen, Pedro García-Caparros, Tao Hu","doi":"10.1093/hr/uhae293","DOIUrl":"https://doi.org/10.1093/hr/uhae293","url":null,"abstract":"Grasses, including turf and forage, cover most of the earth’s surface; predominantly important for land, water, livestock feed, soil and water conservation, as well as carbon sequestration. Improved production and quality of grasses by modern molecular breeding is gaining more research attention. Recent advances in genome-editing technologies are helping to revolutionize plant breeding and also offering smart and efficient acceleration on grass improvement. Here, we reviewed all recent researches using (CRISPR)/CRISPR-associated protein (Cas)-mediated genome editing tools to enhance the growth and quality of forage and turf grasses. Furthermore, we highlighted emerging approaches aimed at advancing grass breeding program. We assessed the CRISPR-Cas effectiveness, discussed the challenges associated with its application, and explored future perspectives primarily focusing on turf and forage grasses. Despite the promising potential of genome editing in grasses, its current efficiency remains limited due to several bottlenecks, such as the absence of comprehensive reference genomes, the lack of efficient gene delivery tools, unavailability of suitable vector and delivery for grass species, high polyploidization, multiple homoeoalleles, etc. Despite these challenges, the CRISPR-Cas system holds great potential to fully harness its benefits in grass breeding and genetics, aiming to improve and sustain the quantity and quality of turf and forage grasses.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"208 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis CsNAC17 通过与山茶中的 CsbHLH62 相互作用，增强山茶对球孢子菌的抗性

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-14 DOI: 10.1093/hr/uhae295

Rui Han, Huiling Mei, Qiwei Huang, Cunqiang Ma, Yuxin Zhao, Anbuaj Jeyaraj, Jing Zhuang, Yuhua Wang, Xuan Chen, Shujing Liu, Xinghui Li

{"title":"CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis","authors":"Rui Han, Huiling Mei, Qiwei Huang, Cunqiang Ma, Yuxin Zhao, Anbuaj Jeyaraj, Jing Zhuang, Yuhua Wang, Xuan Chen, Shujing Liu, Xinghui Li","doi":"10.1093/hr/uhae295","DOIUrl":"https://doi.org/10.1093/hr/uhae295","url":null,"abstract":"The pathogen Colletotrichum gloeosporioides causes anthracnose, a serious threat to tea trees around the world, particularly in warm and humid regions. RNA-Seq data have previously indicated NAC transcription factors are involved in anthracnose resistance, but underlying mechanisms remain unclear. The BiFC, Split-LUC, and Co-IP assays validated the interaction between CsbHLH62 and CsNAC17 identified through yeast two-hybrid (Y2H) screening. CsNAC17 or CsbHLH62 overexpression enhanced anthracnose resistance, as well as enhanced levels of H2O2, hypersensitivity, and cell death in Nicotiana benthamiana. The NBS-LRR gene CsRPM1 is regulated by CsNAC17 by binding directly to its promoter (i.e., CACG, CATGTG), while CsbHLH62 facilitates CsNAC17’s binding and increases trascriptional activity of CsRPM1. Additionally, transient silencing of CsNAC17 and CsbHLH62 in tea plant leaves using the virus-induced gene silencing (VIGS) system resulted in decreased resistance to anthracnose. Conversely, transient overexpression of CsNAC17 and CsbHLH62 in tea leaves significantly enhanced the resistance against anthracnose. Based on these results, it appears that CsbHLH62 facilitates the activity of CsNAC17 on CsRPM1, contributing to increased anthracnose resistance.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"19 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue Culture-Independent Approaches to Revolutionizing Plant Transformation and Gene Editing 彻底改变植物转化和基因编辑的组织培养独立方法

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2024-10-14 DOI: 10.1093/hr/uhae292

Luis Felipe Quiroz, Moman Khan, Nikita Gondalia, Linyi Lai, Peter C McKeown, Galina Brychkova, Charles Spillane

{"title":"Tissue Culture-Independent Approaches to Revolutionizing Plant Transformation and Gene Editing","authors":"Luis Felipe Quiroz, Moman Khan, Nikita Gondalia, Linyi Lai, Peter C McKeown, Galina Brychkova, Charles Spillane","doi":"10.1093/hr/uhae292","DOIUrl":"https://doi.org/10.1093/hr/uhae292","url":null,"abstract":"Despite the transformative power of gene editing for crop improvement, its widespread application across species and varieties is limited by the transformation bottleneck that exists for many crops. The genetic transformation of plants is hindered by a general reliance on in vitro regeneration through plant tissue culture. Tissue culture requires empirically-determined conditions and aseptic techniques, and cannot easily be translated to recalcitrant species and genotypes. Both Agrobacterium-mediated and alternative transformation protocols are limited by a dependency on in vitro regeneration, which also limits their use by non-experts and hinders research into non-model species such as those of possible novel biopharmaceutical or nutraceutical use, as well as novel ornamental varieties. Hence, there is significant interest in developing tissue culture-independent plant transformation and gene editing approaches which can circumvent the bottlenecks associated with in vitro plant regeneration recalcitrance. Compared to tissue culture-based transformations, tissue culture-independent approaches offer advantages such as avoidance of somaclonal variation effects, with more streamlined and expeditious methodological processes. The ease of use, dependability, and accessibility of tissue culture-independent procedures can make them attractive to non-experts, outperforming classic tissue culture-dependent systems. This review explores the diversity of tissue culture-independent transformation approaches and compares them to traditional tissue culture-dependent transformation strategies. We highlight their simplicity and provide examples of recent successful transformations accomplished using these systems. Our review also addresses current limitations and explores future perspectives, highlighting the significance of these techniques for advancing plant research and crop improvement.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"19 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0