Horticulture Research最新文献_第3页

Plant resistance inducer AMHA enhances antioxidant capacities to promote cold tolerance by regulating the upgrade of glutathione S-transferase in tea plant

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-05 DOI: 10.1093/hr/uhaf073

Xuejin Chen, Ning Zhou, Lisha Yu, Zhaolan Han, Yanjing Guo, Salome Njeri Ndombi, Huan Zhang, Jie Jiang, Yu Duan, Zhongwei Zou, Yuanchun Ma, Xujun Zhu, Shiguo Chen, Wanping Fang

{"title":"Plant resistance inducer AMHA enhances antioxidant capacities to promote cold tolerance by regulating the upgrade of glutathione S-transferase in tea plant","authors":"Xuejin Chen, Ning Zhou, Lisha Yu, Zhaolan Han, Yanjing Guo, Salome Njeri Ndombi, Huan Zhang, Jie Jiang, Yu Duan, Zhongwei Zou, Yuanchun Ma, Xujun Zhu, Shiguo Chen, Wanping Fang","doi":"10.1093/hr/uhaf073","DOIUrl":"https://doi.org/10.1093/hr/uhaf073","url":null,"abstract":"Plant resistance inducers represent an alternative strategy that mitigate stress-induced damage in plants. Previously, 2-Amino-3-methylhexanoic Acid (AMHA), a novel natural plant resistance inducer, was shown to significantly bolster cold tolerance, thermotolerance and pathogen resistance in plants. However, the intricate mechanisms underlying AMHA’s response to cold stress remain elusive. Thus, we investigated the physiological and transcriptomic analyses of AMHA pretreatment on tea plant to determine the substantial role of AMHA under cold stress. The results showed that pretreatment with 100 nM AMHA effectively mitigated the detrimental effects of cold stress on photosynthesis and growth. Furthermore, differentially expressed genes were identified through RNA-seq during pretreatment, cold stress, and 2 days (2 d) of recovery. These genes were mainly enriched in pathways related to flavonoid/anthocyanin, carotenoid, and ascorbic acid-glutathione (AsA-GSH) cycle, including GST (encoding glutathione S-transferase). Potential regulatory relationships between the identified genes and transcription factors were also established. Antisense oligodeoxynucleotide-silencing and overexpression experiments revealed that CsGSTU7 enhances cold resistance by maintaining redox homeostasis. In conclusion, our study suggests that antioxidant-related signal molecules play a critical role in the signal cascades and transcriptional regulation mediating AMHA-induced cold-stress resistance in tea plant.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"28 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of the super pan-genome for the genus Actinidia reveals structural variations linked to phenotypic diversity

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-04 DOI: 10.1093/hr/uhaf067

Haolin Wu, Wenjie Yang, Guanyong Dong, Quanjun Hu, Dawei Li, Jianquan Liu

{"title":"Construction of the super pan-genome for the genus Actinidia reveals structural variations linked to phenotypic diversity","authors":"Haolin Wu, Wenjie Yang, Guanyong Dong, Quanjun Hu, Dawei Li, Jianquan Liu","doi":"10.1093/hr/uhaf067","DOIUrl":"https://doi.org/10.1093/hr/uhaf067","url":null,"abstract":"Kiwifruits, belonging to the genus Actinidia, are acknowledged as one of the most successfully domesticated fruits in the 20th century. Despite the rich wild resources and diverse phenotypes within this genus, insights into the genomic changes are still limited. Here, we conducted whole-genome sequencing on seven representative materials from highly diversified sections of Actinidia, leading to the assembly and annotation of 14 haplotype genomes with sizes spanning from 602.0 to 699.6 Mb. By compiling these haplotype genomes, we constructed a super pan-genome for the genus. We identified numerous structural variations (SVs, including variations in gene copy number) and highly diverged regions in these genomes. Notably, significant SV variability was observed within the intronic regions of the MED25 and TTG1 genes across different materials, suggesting their potential roles in influencing fruit size and trichome formation. Intriguingly, our findings indicated a high genetic divergence between two haplotype genomes, with one individual, tentatively named Actinidia × leiocacarpae, from sect. Leiocacarpae. This likely hybrid with a heterozygous genome exhibited notable genetic adaptations related to resistance against bacterial canker, particularly through the upregulation of the RPM1 gene, which contains a specific SV, after infection by Pseudomonas syringae pv. actinidiae. In addition, we also discussed the inter-lineage hybridizations and taxonomic treatments of the genus Actinidia. Overall, the comprehensive pan-genome constructed here, along with our findings, lays a foundation for examining genetic compositions and markers, particularly those related to SVs, to facilitate hybrid breeding aimed at developing desired phenotypes in kiwifruits.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"35 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanism of SmMYB53 activates the expression of SmCYP71D375 thereby modulating the tanshinones accumulation in salvia miltiorrhiza

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-04 DOI: 10.1093/hr/uhaf058

Xinyu Wang, Yifei Shi, Qichao Wang, Xinjia Xie, Siqi Gui, Jiening Wu, Limei Zhao, Xiaowei Zou, Guoyin Kai, Wei Zhou

{"title":"Molecular mechanism of SmMYB53 activates the expression of SmCYP71D375 thereby modulating the tanshinones accumulation in salvia miltiorrhiza","authors":"Xinyu Wang, Yifei Shi, Qichao Wang, Xinjia Xie, Siqi Gui, Jiening Wu, Limei Zhao, Xiaowei Zou, Guoyin Kai, Wei Zhou","doi":"10.1093/hr/uhaf058","DOIUrl":"https://doi.org/10.1093/hr/uhaf058","url":null,"abstract":"Tanshinones are the bioactive diterpenoid chemicals of the herb Salvia miltiorrhiza with the characteristic furan D-ring. As a newly identified downstream enzyme, SmCYP71D375 catalyzes hydroxylation at 14,16-ether (hetero) cyclization to form the furan D-ring from the precursor of phenolic abietane-type diterpenoids widely exists in the Lamiaceae plants. But its transcriptional regulatory network with SmCYP71D375 as the direct target gene, remains unclear. In present study, the promoter of SmCYP71D375 is employed to be the bait to mine the upstream regulatory protein using the cDNA yeast library of S. miltiorrhiza. An R2R3-MYB transcription factor, SmMYB53, was identified. Overexpressing SmMYB53 in transgenic hairy roots upregulate the SmCYP71D375 expression thereby accelerating the tanshinones accumulation. Whereas, the tanshinones accumulation is inhibited in SmMYB53-RNAi transgenic hairy root lines. To dissect its regulatory network of SmMYB53, SmbZIP51 was captured using SmMYB53 as the bait to prey its potential interacting proteins in the cDNA yeast library. Yeast two-hybrid, GST pull-down and bimolecular fluorescence complementation assays were independently introduced to verify their interaction between the proteins of SmMYB53 and SmbZIP51. We further verified that the upregulation of SmCYP71D375 activated by SmMYB53 would be inhibited by their interaction of SmMYB53 and SmbZIP51. The present findings uncover the molecular regulatory network underlying SmCYP71D375 as the direct target to regulate tanshinones biosynthesis and offer the basis for the genetic improvement of medicinal substances biosynthesis in S. miltiorrhiza.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"131 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root-specific expression of CsNPF2.3 is involved in modulating fluoride accumulation in tea plant (Camellia sinensis)

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-04 DOI: 10.1093/hr/uhaf072

Huiliang Niu, Junjie Wang, Zhiwei Liao, Yangjuan Deng, Qi Chen, Chuanyi Peng, Guijie Chen, Ruyan Hou, Xiaochun Wan, Zhaoliang Zhang, Huimei Cai

{"title":"Root-specific expression of CsNPF2.3 is involved in modulating fluoride accumulation in tea plant (Camellia sinensis)","authors":"Huiliang Niu, Junjie Wang, Zhiwei Liao, Yangjuan Deng, Qi Chen, Chuanyi Peng, Guijie Chen, Ruyan Hou, Xiaochun Wan, Zhaoliang Zhang, Huimei Cai","doi":"10.1093/hr/uhaf072","DOIUrl":"https://doi.org/10.1093/hr/uhaf072","url":null,"abstract":"F is a non-essential but potentially harmful element for plants, especially when present in excess. The tea plant is known for its ability to hyperaccumulate F from the soil and eventually accumulates in the leaves; however, how tea plant transport F to the leaves remains unclear. Here, we found that Se can significantly decrease the transport efficiency of F from root to leaf. Therefore, RNA-Sequencing was performed on tea roots co-treated with selenite and fluoride, and then we isolated a plasma membrane-localized F transporter CsNPF2.3 from tea plant roots and examined its role in transport of F in tea plant. The results showed that CsNPF2.3 exhibited F transport activity when heterologously expressed in yeast. Expression pattern analysis revealed that CsNPF2.3 is expressed in epidermal cells, cortex cells and xylem parenchyma cells in roots. Overexpression of CsNPF2.3 in tea roots significantly increased F content in the root, stem and leaf, and enhanced the transport efficiency of F from root to leaf. Furthermore, in 9 tea cultivars, CsNPF2.3 expression in the root was significantly positively correlated with F content in the leaf and root, and the transport efficiency of F from root to leaf. Altogether, these findings suggest that CsNPF2.3 was involved in uptake and transport of F in tea plant.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"30 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Glycolysis: Multifunctional Roles of Glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) in Plants

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-04 DOI: 10.1093/hr/uhaf070

Yunpeng Cao, Jiayi Hong, Han Wang, Mengfei Lin, Yongping Cai, Liao Liao, Xiaoxu Li, Yuepeng Han

引用次数: 0

Characterization of a KANADI-like transcription factor that suppresses pear anthocyanin biosynthesis

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-03-04 DOI: 10.1093/hr/uhaf071

Weilin Wei, Kui Lin-Wang, Guosong Chen, Richard V Espley, Andrew C Allan, Beibei Cao, Mengfan Qin, Shoufeng Sha, Juncai Li, Runze Wang, Jiaming Li, Jun Wu

{"title":"Characterization of a KANADI-like transcription factor that suppresses pear anthocyanin biosynthesis","authors":"Weilin Wei, Kui Lin-Wang, Guosong Chen, Richard V Espley, Andrew C Allan, Beibei Cao, Mengfan Qin, Shoufeng Sha, Juncai Li, Runze Wang, Jiaming Li, Jun Wu","doi":"10.1093/hr/uhaf071","DOIUrl":"https://doi.org/10.1093/hr/uhaf071","url":null,"abstract":"Anthocyanins are important specialized fruit metabolites and major pigments, whose abundance depends on co-regulation of activators and repressors, primarily transcription factors (TFs) of the MYB family. Herein, a KANADI-like TF PuKAN4 was characterized in pear. This TF could be transcriptionally up-regulated by the anthocyanin-related R2R3-MYBs PuMYB10/PuMYB114 and exhibited high expression within red-skinned pears. Interestingly, PuKAN4 repressed anthocyanin biosynthesis in transiently overexpressed pear fruit, and stable transformation in pear calli and tobacco plants. The PuKAN4 had a conserved EAR repression domain in C-terminal, while the repression function of PuKAN4 could be offset by a transcription activation domain VP64. The dual luciferase analysis proved that PuMYB114/PuMYB10 up-regulated expression of PuKAN4. Furthermore, the PuKAN4 could physically interact with PuMYB10/PuMYB114 and did not affect the combination of MYB10/MYB114-bHLH3, as demonstrated by Y2H, pull-down and firefly luciferase complementation. Thus, the PuKAN4 should play the role of active repressor, the formation of PuKAN4-PuMYB10/PuMYB114-PubHLH3 complex inhibited pear anthocyanin biosynthesis. Our findings unveiled an activator-and-repressor feedback loop between PuMYB114/PuMYB10 and PuKAN4, which possibly balance biosynthesis activity and prevent over-accumulation of phenylpropanoids.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"13 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal fruit microbiome and immunity dynamics in post-harvest apple (Malus x domestica)

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-25 DOI: 10.1093/hr/uhaf063

Roselane Kithan-Lundquist, Hannah M McMillan, Sheng-Yang He, George W Sundin

{"title":"Temporal fruit microbiome and immunity dynamics in post-harvest apple (Malus x domestica)","authors":"Roselane Kithan-Lundquist, Hannah M McMillan, Sheng-Yang He, George W Sundin","doi":"10.1093/hr/uhaf063","DOIUrl":"https://doi.org/10.1093/hr/uhaf063","url":null,"abstract":"The plant immune response plays a central role in maintaining a well-balanced and healthy microbiome for plant health. However, insights on how the fruit immune response and the fruit microbiome influence fruit health after harvest are limited. We investigated the temporal dynamics of the fruit microbiota and host defense gene expression patterns during post-harvest storage of apple fruits at room temperature. Our results demonstrate a temporal dynamic shift in both bacterial and fungal community composition during post-harvest storage that coincides with a steep-decline in host defense response gene expression associated with pattern-triggered immunity. We observed the gradual appearance of putative pathogenic/spoilage microbes belonging to genera Alternaria (fungi) and Gluconobacter and Acetobacter (bacteria) at the expense of Sporobolomyces and other genera, which have been suggested to be beneficial for plant hosts. Moreover, artificial induction of pattern-triggered immunity in apple fruit with the flg22 peptide delayed the onset of fruit rot caused by the fungal pathogen Penicillium expansum. Our results suggest that the fruit immune response helps to orchestrate a microbiome and that the collapse of the immunity results in the proliferation of spoilage microbes and fruit rot. These findings hold implications for the development of strategies to increase fruit quality and prolong shelf life in fruits and vegetables.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"27 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143495163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental landscape and asymmetric gene expression in the leaf vasculature of Brassica rapa revealed by single-cell transcriptome

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-25 DOI: 10.1093/hr/uhaf060

Xinlei Guo, Jingping Yuan, Yuanyuan Zhang, Jian Wu, Xiaowu Wang

{"title":"Developmental landscape and asymmetric gene expression in the leaf vasculature of Brassica rapa revealed by single-cell transcriptome","authors":"Xinlei Guo, Jingping Yuan, Yuanyuan Zhang, Jian Wu, Xiaowu Wang","doi":"10.1093/hr/uhaf060","DOIUrl":"https://doi.org/10.1093/hr/uhaf060","url":null,"abstract":"Leaf vasculature not only acts as a channel for nutrients and signaling information but also influences leaf morphology. It consists of several distinct cell types with specialized functions. Cell type-specific characterizations based on single-cell RNA sequencing technology could aid in understanding the identities of vascular tissues and their roles in leaf morphogenesis in Brassica rapa. Here, we generated a single-cell transcriptome landscape of the Chinese cabbage leaf vasculature. A total of 12 cell clusters covering 7 known cell types were identified. Different vascular cell types were characterized by distinct identities. The xylem parenchyma and companion cells exhibited an active expression pattern of amino acid metabolism genes. Tracheary elements and sieve elements were enriched in many genes related to cell wall biosynthesis, and the phloem parenchyma was enriched in many sugar transporter-encoding genes. Pseudo-time analyses revealed the developmental trajectories of the xylem and phloem and the potential roles of auxin and ethylene in xylem development. Furthermore, we identified key candidate regulators along the differentiation trajectory of the sieve elements and companion cells. Most of the homoeologous genes in the syntenic triads from the three subgenomes showed asymmetric gene expression patterns in different vascular cell types. Collectively, our study revealed that Chinese cabbage leaf vasculature cells had highly heterogeneous transcriptomes, providing new insights into the complex processes of leaf vasculature development in B. rapa leafy vegetables and other Brasscia crops.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"66 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143495171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stable isotope labelling and gene expression analysis reveal dynamic nitrogen-supply mechanisms for rapid growth of Moso bamboo

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-25 DOI: 10.1093/hr/uhaf062

Junbo Zhang, Man Shi, Chenglei Zhu, Kebin Yang, Quan Li, Xiaoming Song, Zhimin Gao, Tingting Cao, Dezheng Zhu, Xinzhang Song

{"title":"Stable isotope labelling and gene expression analysis reveal dynamic nitrogen-supply mechanisms for rapid growth of Moso bamboo","authors":"Junbo Zhang, Man Shi, Chenglei Zhu, Kebin Yang, Quan Li, Xiaoming Song, Zhimin Gao, Tingting Cao, Dezheng Zhu, Xinzhang Song","doi":"10.1093/hr/uhaf062","DOIUrl":"https://doi.org/10.1093/hr/uhaf062","url":null,"abstract":"Rapid growth of Moso bamboo (Phyllostachys edulis) shoots (offspring ramet) is primarily fuelled by nitrogen (N) derived from parent ramet and absorbed by rhizome roots. However, the extent to which each N source supports the growth of offspring ramet and the underlying molecular mechanisms of N transport remain unclear. Here, clonal fragments consisting of a parent ramet, an offspring ramet, and an interconnected rhizome were established in a Moso bamboo forest. Additionally, 15N isotope tracing and transcriptome profiling were conducted concurrently to quantify the N contribution from the parent ramet and rhizome roots to the offspring ramet, and to reveal the molecular mechanisms underlying N transport during rapid growth (i.e., early, peak, branching, and leafing stages). The N acquisition strategy of offspring ramet shifted from being primarily provided by the parent ramet (72.53%) during early stage to being predominantly absorbed by rhizome roots (69.85%) during the leafing stage. Approximately equal N contributions (45.82–54.18%) from the parent ramet and rhizome roots were observed during peak and branching stages. PeAAP29123 was identified as a key gene for N transport, being most closely correlated with 15N content. Biomolecular assays demonstrated that PeHDZ23987 could activate the expression of PeAAP29123 via two types of HD-motifs. Overexpression of PeHDZ23987 and PeAAP29123 significantly enhanced N starvation tolerance in transgenic rice with significantly improved N uptake efficiency. Our findings clarify the pattern and mechanisms of N supply for the rapid growth of Moso bamboo offspring ramet and provide transcriptomic evidence for long-distance N transport between clonal ramets.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"23 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143495164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethylene Modulates the Phenylpropanoid Pathway by Enhancing VvMYB14 Expression via the ERF5-Melatonin-ERF104 Pathway in Grape Seeds

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-02-25 DOI: 10.1093/hr/uhaf061

Shiwei Gao, Fei Wang, Shengnan Wang, Jiapeng Diao, Shuxia Lan, Yujiao Xu, Xinning Lyu, Hui Kang, Yuxin Yao

{"title":"Ethylene Modulates the Phenylpropanoid Pathway by Enhancing VvMYB14 Expression via the ERF5-Melatonin-ERF104 Pathway in Grape Seeds","authors":"Shiwei Gao, Fei Wang, Shengnan Wang, Jiapeng Diao, Shuxia Lan, Yujiao Xu, Xinning Lyu, Hui Kang, Yuxin Yao","doi":"10.1093/hr/uhaf061","DOIUrl":"https://doi.org/10.1093/hr/uhaf061","url":null,"abstract":"The interaction between ethylene and melatonin in the regulation of polyphenol metabolism and the underlying mechanism remain largely unclear. This work demonstrated that ethylene treatment increased melatonin biosynthesis by inducing the VvASMT expression in grape seeds. Ethylene-induced VvERF5 transactivated VvASMT via binding to the ERE element in its promoter. VvERF5 overexpression led to an increase in melatonin biosynthesis while its suppression generated the opposite results in grape seeds, calli and/or Arabidopsis seeds. A melatonin responsive element (MTRE) was identified, and melatonin-induced VvERF104 was found to bind to the MTRE of the VvMYB14 promoter and activate its expression. VvMYB14 overexpression widely modified the expression of genes in phenylpropanoid pathway and phenolic compound content in grape seeds. DAP-seq revealed that the MEME-1 motif was the most likely binding sites of VvMYB14. VvPAL, VvC4H and VvCHS were verified to be the target genes of VvMYB14. Additionally, the overexpression of VvERF5 or VvERF104 increased the expression of VvPAL, VvC4H and VvCHS, as well as the levels of the corresponding metabolites. Moreover, the roles of VvERF5, VvASMT and VvERF104 in mediating ethylene-induced changes in phenylpropanoid pathway were elucidated using their suppressing seeds. Collectively, ethylene increased the VvMYB14 expression via the pathway of ERF5-melatonin-ERF104 and thereby modified phenylpropanoid pathway.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"27 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143495168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0